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WO2006033769A3 - Assays and compositions for identification of cancer associated liver stem cells - Google Patents

Assays and compositions for identification of cancer associated liver stem cells Download PDF

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Publication number
WO2006033769A3
WO2006033769A3 PCT/US2005/030335 US2005030335W WO2006033769A3 WO 2006033769 A3 WO2006033769 A3 WO 2006033769A3 US 2005030335 W US2005030335 W US 2005030335W WO 2006033769 A3 WO2006033769 A3 WO 2006033769A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cells
liver stem
assays
compositions
identification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2005/030335
Other languages
French (fr)
Other versions
WO2006033769A2 (en
Inventor
Catherine Shachaf
Dean W Felsher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leland Stanford Junior University
Original Assignee
Leland Stanford Junior University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leland Stanford Junior University filed Critical Leland Stanford Junior University
Publication of WO2006033769A2 publication Critical patent/WO2006033769A2/en
Publication of WO2006033769A3 publication Critical patent/WO2006033769A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A transgenic animal model for liver cancer and liver stem cells derived therefrom are provided. Animals are genetically modified to comprise an oncogene that is conditionally expressed in liver cells. After induction of expression, and tumor formation, expression of the oncogene can be inactivated, resulting in regression of the tumor, and generation of a population of liver stem cells with latent tumorigenic properties.
PCT/US2005/030335 2004-08-26 2005-08-25 Assays and compositions for identification of cancer associated liver stem cells Ceased WO2006033769A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60509604P 2004-08-26 2004-08-26
US60/605,096 2004-08-26

Publications (2)

Publication Number Publication Date
WO2006033769A2 WO2006033769A2 (en) 2006-03-30
WO2006033769A3 true WO2006033769A3 (en) 2006-11-30

Family

ID=36090444

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/030335 Ceased WO2006033769A2 (en) 2004-08-26 2005-08-25 Assays and compositions for identification of cancer associated liver stem cells

Country Status (1)

Country Link
WO (1) WO2006033769A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109097335B (en) * 2018-08-29 2022-08-19 刘卫辉 Method for inducing malignant transformation from normal liver stem cell to liver cancer stem cell

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0589851A1 (en) * 1992-09-07 1994-03-30 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Transgenic non-human mammals with an oncogene under the control of an inducible liver-specific promoter

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0589851A1 (en) * 1992-09-07 1994-03-30 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Transgenic non-human mammals with an oncogene under the control of an inducible liver-specific promoter

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CARTIER N. ET AL.: "Diet-Dependent Carcinogenesis of Pancreatic Islets and Liver in Transgenic Mice Expressing Oncogenes Under the Control of the L-Type Pyruvate Kinase Gene Promoter", ONCOGENE, vol. 7, no. 7, July 1992 (1992-07-01), pages 1413 - 1422, XP008073343 *
FACTOR V.M. ET AL.: "Coexpression of C-myc and Transforming Growth Factor Alpha in the Liver Promotes Early Replicative Senescence and Dimnishes Regenerative Capacity After Partial Hepatectomy in Transgenic Mice", HEPATOLOGY, vol. 26, 1997, pages 1434 - 1443, XP003004402 *
GALLAGHER A. ET AL.: "Use of the Tetracycline System for Inducible Protein Synthesis in the Kidney", J. AM. SOC. NEPHROL., vol. 14, 2003, pages 2042 - 2051, XP003004404 *
OHGAKI H. ET AL.: "Molecular Analyses of Liver Tumors in c-myc Transgenic Mice and c-myc and TGF-a Double Transgenic Mice", CANCER LETTERS, vol. 106, 1996, pages 43 - 49, XP003004403 *

Also Published As

Publication number Publication date
WO2006033769A2 (en) 2006-03-30

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