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WO2006032504A3 - Method for introducing genetic mutations into plant cells - Google Patents

Method for introducing genetic mutations into plant cells Download PDF

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Publication number
WO2006032504A3
WO2006032504A3 PCT/EP2005/010270 EP2005010270W WO2006032504A3 WO 2006032504 A3 WO2006032504 A3 WO 2006032504A3 EP 2005010270 W EP2005010270 W EP 2005010270W WO 2006032504 A3 WO2006032504 A3 WO 2006032504A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
plant
genetic mutations
plant cell
plant cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2005/010270
Other languages
French (fr)
Other versions
WO2006032504A2 (en
Inventor
Gabriela Ispas
Ivan Famelaer
Geert Angenon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vrije Universiteit Brussel VUB
Universite Libre de Bruxelles ULB
Original Assignee
Vrije Universiteit Brussel VUB
Universite Libre de Bruxelles ULB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vrije Universiteit Brussel VUB, Universite Libre de Bruxelles ULB filed Critical Vrije Universiteit Brussel VUB
Publication of WO2006032504A2 publication Critical patent/WO2006032504A2/en
Publication of WO2006032504A3 publication Critical patent/WO2006032504A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides a method for introducing genetic mutations into the genome of plant cells, comprising the steps of: (i.) suppressing the activity of at least one endogenous system of DNA repair in said at least one plant cell; (ii.) exposing said at least one plant cell to at least one mutagenic agent. In particular embodiments of the present invention the mismatch repair system is suppressed in said plant cell, and a DNA-alkylating agent is used for mutagenesis. The present invention aspires to enable the generation of libraries of mutants of various plant species, wherein a greater proportion of the genes will be containing a mutation, and wherein a greater number of different phenotypes will be obtained than achieved using current methods. This effect of the present invention will help close the 'phenotypic gap' present in the existing libraries of plant mutants.
PCT/EP2005/010270 2004-09-22 2005-09-22 Method for introducing genetic mutations into plant cells Ceased WO2006032504A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP2004010617 2004-09-22
EPPCT/EP2004/010617 2004-09-22

Publications (2)

Publication Number Publication Date
WO2006032504A2 WO2006032504A2 (en) 2006-03-30
WO2006032504A3 true WO2006032504A3 (en) 2006-07-13

Family

ID=36084413

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/010270 Ceased WO2006032504A2 (en) 2004-09-22 2005-09-22 Method for introducing genetic mutations into plant cells

Country Status (1)

Country Link
WO (1) WO2006032504A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101597328B (en) * 2008-06-06 2012-07-11 中国科学院遗传与发育生物学研究所 Transcription factors promoting plant somatic embryogenesis and fatty acid synthesis and their encoding genes and applications
WO2010074562A1 (en) * 2008-12-22 2010-07-01 Keygene N.V. Use of double stranded rna to increase the efficiency of targeted gene alteration in plant protoplasts
ES2583060T3 (en) * 2009-12-21 2016-09-16 Keygene N.V. Enhanced techniques for protoplast transfection
CN102812803B (en) * 2012-07-02 2016-02-24 青岛市农业科学研究院 A kind of method that pea EMS mutant library builds
US10595479B2 (en) * 2014-04-24 2020-03-24 Purdue Research Foundation Induced mutagenesis

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997005268A1 (en) * 1995-07-26 1997-02-13 Setratech Homologous recombination in mismatch repair inactivated eukaryotic cells
WO1999019492A2 (en) * 1997-10-10 1999-04-22 Aventis Cropscience S.A. Methods for obtaining plant varieties
WO2001059092A2 (en) * 2000-02-11 2001-08-16 The Johns Hopkins University Methods for generating hypermutable bacteria
WO2001061012A1 (en) * 2000-02-18 2001-08-23 Nicolaides Nicholas C A method for generating hypermutable plants
WO2001088192A2 (en) * 2000-05-12 2001-11-22 The Johns Hopkins University A method for generating hypermutable organisms
WO2002054856A1 (en) * 2001-01-15 2002-07-18 Morphotek Inc. Chemical inhibitors of mismatch repair
WO2003054217A2 (en) * 2001-12-20 2003-07-03 Boyce Thompson Institute For Plant Research Plant msh2 sequences and methods of use

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997005268A1 (en) * 1995-07-26 1997-02-13 Setratech Homologous recombination in mismatch repair inactivated eukaryotic cells
WO1999019492A2 (en) * 1997-10-10 1999-04-22 Aventis Cropscience S.A. Methods for obtaining plant varieties
WO2001059092A2 (en) * 2000-02-11 2001-08-16 The Johns Hopkins University Methods for generating hypermutable bacteria
WO2001061012A1 (en) * 2000-02-18 2001-08-23 Nicolaides Nicholas C A method for generating hypermutable plants
WO2001088192A2 (en) * 2000-05-12 2001-11-22 The Johns Hopkins University A method for generating hypermutable organisms
WO2002054856A1 (en) * 2001-01-15 2002-07-18 Morphotek Inc. Chemical inhibitors of mismatch repair
WO2003054217A2 (en) * 2001-12-20 2003-07-03 Boyce Thompson Institute For Plant Research Plant msh2 sequences and methods of use

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ADE JULES ET AL: "Functional analysis of the Arabidopsis thaliana mismatch repair gene MSH2", GENOME, vol. 44, no. 4, August 2001 (2001-08-01), pages 651 - 657, XP001208017, ISSN: 0831-2796 *
ANDREW SUSAN E ET AL: "Tissues of MSH2-deficient mice demonstrate hypermutability on exposure to a DNA methylating agent", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 95, no. 3, 3 February 1998 (1998-02-03), pages 1126 - 1130, XP002375421, ISSN: 0027-8424 *
HOFFMAN PETER D ET AL: "Rapid accumulation of mutations during seed-to-seed propagation of mismatch-repair-defective Arabidopsis", GENES & DEVELOPMENT, vol. 18, no. 21, 1 November 2004 (2004-11-01), pages 2676 - 2685, XP002375419, ISSN: 0890-9369 *
ISPAS GABRIELA ET AL: "Interferance of Mismatch Repair System on genetic recombination in plants.", MEDEDELINGEN FACULTEIT LANDBOUWKUNDIGE EN TOEGEPASTE BIOLOGISCHE WETENSCHAPPEN UNIVERSITEIT GENT, vol. 67, no. 4, 2002, pages 239 - 241, XP008062435, ISSN: 1373-7503 *
LEONARD JEFFREY M ET AL: "Reduction of stability of Arabidopsis genomic and transgenic dna-repeat sequences (Microsatellites) by inactivation of AtMSH2 mismatch-repair function.", PLANT PHYSIOLOGY (ROCKVILLE), vol. 133, no. 1, September 2003 (2003-09-01), pages 328 - 338, XP002375417, ISSN: 0032-0889 *
RAKOSY-TICAN LENUTA ET AL: "Transformation of wild Solanum species resistant to late blight by using reporter gene gfp and msh2 genes", PLANT BREEDING AND SEED SCIENCE, vol. 50, 2004, pages 119 - 127, XP008062469, ISSN: 1429-3862 *
STUDAMIRE BARBARA ET AL: "Separation-of-function mutations in Saccharomyces cerevisiae MSH2 that confer mismatch repair defects but do not affect nonhomologous-tail removal during recombination", MOLECULAR AND CELLULAR BIOLOGY, vol. 19, no. 11, November 1999 (1999-11-01), pages 7558 - 7567, XP002375528, ISSN: 0270-7306 *

Also Published As

Publication number Publication date
WO2006032504A2 (en) 2006-03-30

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