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WO2006031105A1 - Plaque de titration et dispositif de mesure pour analyse chimique et/ou biochimique - Google Patents

Plaque de titration et dispositif de mesure pour analyse chimique et/ou biochimique Download PDF

Info

Publication number
WO2006031105A1
WO2006031105A1 PCT/NL2005/000665 NL2005000665W WO2006031105A1 WO 2006031105 A1 WO2006031105 A1 WO 2006031105A1 NL 2005000665 W NL2005000665 W NL 2005000665W WO 2006031105 A1 WO2006031105 A1 WO 2006031105A1
Authority
WO
WIPO (PCT)
Prior art keywords
titre plate
metallic layer
reaction chambers
measuring device
plate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/NL2005/000665
Other languages
English (en)
Inventor
Adrianus Bossche
Vladimir Gueorguiev Kutchoukov
Yuval Garini
Ian Theodore Young
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Technische Universiteit Delft
Original Assignee
Technische Universiteit Delft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technische Universiteit Delft filed Critical Technische Universiteit Delft
Publication of WO2006031105A1 publication Critical patent/WO2006031105A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G01N21/553—Attenuated total reflection and using surface plasmons

Definitions

  • the invention relates to a titre plate for chemical and/or biochemical analysis.
  • Such a titre plate is known from practice, for example, in the form of a so-called nanolitre titre plate, which provides a possibility of carrying out analyses using approximately ten reaction chambers per mm 2 of plate surface.
  • One object of the invention is to realise a further improvement of the efficiency by significantly raising the number of reaction chambers per unit area and concurrently, to facilitate a simultaneous readout of these reaction cham ⁇ bers.
  • EP-A-I 353 179 relates to a titre plate for chemical and/or biochemical analysis based on an illumination measure ⁇ ment, comprising a transparent substrate, applied to the substrate there is a metallic layer, which is provided with an array of reaction chambers wherein the reaction chambers extend, from the top side of the metallic layer to, or into, the substrate thereunder.
  • the reaction chambers of the titre plate known from this publication are applied in an irregu ⁇ lar pattern and their diameter is at least equal to the wavelength of the applied illumination.
  • the illumi ⁇ nation measurement is based on a reflection measurement.
  • the titre plate according to the invention is characterized by a transparent cover plate at the side of the metallic layer facing away from the sub ⁇ strate, and by reaction chambers whose cross-sectional dimen ⁇ sion, viewed in the plane of the metallic layer, is smaller than the illumination wavelength. Due to both the substrate and the cover plate being transparent so as to allow readout of the reaction chambers to take place by simultaneous illumination through the reac ⁇ tion chambers, the titre plate according to the invention makes it simply and effectively possible to read out all the reaction chambers simultaneously.
  • reaction chambers in the metallic layer It is important for the reaction chambers in the metallic layer to be applied in a regular, periodical pattern and in a density of at least approximately one million per mm .
  • the volume of the reaction chambers to be applied is in the order of one or several attolitres.
  • the titre plate according to the invention is fur ⁇ ther preferably characterised in that the reaction chambers in the plane of the metallic layer have a cross-sectional dimension in the range from 50 to 150 nm.
  • the titre plate according to the invention makes extremely intensive testing possible, at reduced rea- gent consumption.
  • the invention is also embodied in a measuring device for chemical and/or biochemical analysis, comprising a titre plate according to the invention, a light source for illuminating said titre plate and a light measuring device ' .
  • the light sensor is a lens, positioned closely to the titre plate but outside the near field, and cooperating with an optical sensor.
  • the preferred optical sensor to use is a CCD camera, which makes it possible to simultaneously read out the light output per reaction chamber.
  • the distance at which the lens is positioned from the titre plate is preferably in the range from 1 to 1000 ⁇ m.
  • the cover plate and the metallic layer be located on either side of a channel that conducts a sample liquid or washing water.
  • the metallic layer is preferably provided with a hydrophobic coating bordering on the channel.
  • a suitable material for this purpose is, for example, silicon.
  • Such a hydrophobic coating makes a selective supply of reagent and/or sample liquid to the reaction chambers possible. To this end the coating may be applied in a predetermined pat ⁇ tern.
  • reaction chambers In order to be able to perform a simultaneous multi- pie assay of a sample of test liquid, it is desirable for at least one of the reaction chambers to be provided with a predetermined selection from the group comprising antibodies, reagents, DNA.
  • the drawing schematically shows a measuring device provided with a titre plate according to the invention.
  • the schematically illustrated measuring device 1 serves for chemical and/or biochemical analysis and comprises a titre plate 2, a light source 3 for illuminating the titre plate 2 and a lens 4 positioned outside the near field of the titre plate, which cooperates with an optical sensor 5 in the form of, for example, a CCD camera.
  • the lens 4 is positioned close to the titre plate 2, however, not in the near field where the distance would be less than 300 nanometre, but at a distance of 1 to 1000 ⁇ m.
  • the optical sensor cooperating with the lens 4 may be positioned at a greater distance from said lens 4, for example, in the range from 0.1 to 100 cm.
  • the titre plate 2 is characterized by a transparent substrate 6, applied to the substrate 6 there is a metallic layer 7, which is provided with an array of reaction chambers 8, and a transparent cover plate 9 provided at the side of the metallic layer 7 that faces away from the substrate 6.
  • the metallic layer is preferably a gold coating.
  • a further characteristic of the titre plate 2 is that the cover plate 9 and the metallic layer 7 are provided at both sides of a channel 10 for conducting a test liquid or washing water.
  • the metallic layer 7 is preferably also provided with a hydrophobic layer 11 bordering on the channel 10.
  • a suitable material that may be used for this purpose is sili ⁇ con.
  • the material to be used for the transparent sub ⁇ strate 6 may be, for example, glass. Glass may also be used for the cover plate 9.
  • the reaction chambers 8 Prior to bringing the titre plate 2 into the ready form shown in the figure, it is preferred for the reaction chambers 8 to be loaded with a substance previously selected from the group comprising antibodies, DNA, reagent. Further ⁇ more, the figure clearly shows that the reaction chambers 8 extend through the entire thickness of the metallic layer 7. It should be mentioned that occasionally a portion of one or several of the reaction chambers 8 may extend into the mate ⁇ rial of the substrate 6. Some of the material in its surface facing the metallic thin layer 7 may then become etched away.
  • the thin metal layer 7 has a thickness of approximately 100 to 200 nanome- tres, that the reaction chambers 8 in the plane of the metal ⁇ lic layer 7 have an mean size in the range from 50 to 150 nanometres and that the reaction chambers 8 are provided at a density of at least approximately one million per mm 2 of titre plate area.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optical Measuring Cells (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

Cette invention concerne une plaque de titration pour analyse chimique et/ou biochimique reposant sur une mesure d'éclairement comprenant un substrat transparent sur lequel une couche métallique est appliquée, laquelle couche métallique comprend un réseau de chambres de réaction, lesquelles chambres de réaction s'étendent depuis la face supérieure de la couche métallique sur ou dans le substrat situé en dessous. Cette invention se caractérise en ce que la couche métallique comprend, sur son côté orienté dos au substrat, une plaque de revêtement transparente et en ce que la dimension de la section transversale des chambres de réaction, observée dans le plan de la couche métallique, est inférieure à la longueur d'onde d'éclairement.
PCT/NL2005/000665 2004-09-13 2005-09-12 Plaque de titration et dispositif de mesure pour analyse chimique et/ou biochimique Ceased WO2006031105A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL1027031A NL1027031C2 (nl) 2004-09-13 2004-09-13 Titerplaat.
NL1027031 2004-09-13

Publications (1)

Publication Number Publication Date
WO2006031105A1 true WO2006031105A1 (fr) 2006-03-23

Family

ID=34974153

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2005/000665 Ceased WO2006031105A1 (fr) 2004-09-13 2005-09-12 Plaque de titration et dispositif de mesure pour analyse chimique et/ou biochimique

Country Status (2)

Country Link
NL (1) NL1027031C2 (fr)
WO (1) WO2006031105A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0950718A1 (fr) * 1997-07-09 1999-10-20 Dai Nippon Printing Co., Ltd. Procede de detection d'adn au moyen d'une sonde de pna
US20010040679A1 (en) * 1998-05-29 2001-11-15 Yuji Kawabata Surface plasmon resonance sensor apparatus using surface emitting laser
WO2003006948A2 (fr) * 2001-07-10 2003-01-23 Wisconsin Alumni Research Foundation Imagerie par resonance plasmon de surface de microreseaux
WO2003025547A1 (fr) * 2001-09-21 2003-03-27 Biomedlab Corporation Procede et dispositif permettant de balayer des analytes au moyen de resonance plasmonique de surface
EP1353179A1 (fr) * 2001-01-12 2003-10-15 Japan Science and Technology Corporation Element d'analyse par fluorescence au moyen d'un nanopuits metallique et procede de production de cet element
WO2003102559A1 (fr) * 2002-05-31 2003-12-11 Gyros Ab Agencement detecteur utilisant une resonance plasmonique de surface

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0950718A1 (fr) * 1997-07-09 1999-10-20 Dai Nippon Printing Co., Ltd. Procede de detection d'adn au moyen d'une sonde de pna
US20010040679A1 (en) * 1998-05-29 2001-11-15 Yuji Kawabata Surface plasmon resonance sensor apparatus using surface emitting laser
EP1353179A1 (fr) * 2001-01-12 2003-10-15 Japan Science and Technology Corporation Element d'analyse par fluorescence au moyen d'un nanopuits metallique et procede de production de cet element
WO2003006948A2 (fr) * 2001-07-10 2003-01-23 Wisconsin Alumni Research Foundation Imagerie par resonance plasmon de surface de microreseaux
WO2003025547A1 (fr) * 2001-09-21 2003-03-27 Biomedlab Corporation Procede et dispositif permettant de balayer des analytes au moyen de resonance plasmonique de surface
WO2003102559A1 (fr) * 2002-05-31 2003-12-11 Gyros Ab Agencement detecteur utilisant une resonance plasmonique de surface

Also Published As

Publication number Publication date
NL1027031C2 (nl) 2006-03-14

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