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WO2006024283A2 - Compounds and methods for the treatment, diagnosis and prognosis of pancreatic diseases - Google Patents

Compounds and methods for the treatment, diagnosis and prognosis of pancreatic diseases Download PDF

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Publication number
WO2006024283A2
WO2006024283A2 PCT/DE2005/001527 DE2005001527W WO2006024283A2 WO 2006024283 A2 WO2006024283 A2 WO 2006024283A2 DE 2005001527 W DE2005001527 W DE 2005001527W WO 2006024283 A2 WO2006024283 A2 WO 2006024283A2
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chr
protein
gene
gene product
pancreatic
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PCT/DE2005/001527
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WO2006024283A3 (en
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Günter KLÖPPEL
Jutta Lüttges
Holger Kalthoff
Ole Ammerpohl
Robert Grützmann
Christian Pilarsky
Hans-Detlev Saeger
Ingo Alldinger
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Technische Universität Dresden
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Publication of WO2006024283A3 publication Critical patent/WO2006024283A3/en

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    • C—CHEMISTRY; METALLURGY
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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Definitions

  • the invention relates to nucleic acid sequences and peptides or proteins encoded thereby, as well as the use of sequences derived therefrom for screening substances that bind thereto and the use of substances binding to the nucleic acids and peptides or proteins derived therefrom for diagnosis, prognosis, and for the therapeutic treatment of pancreatic tumors, in particular of the ductal adenocarcinoma of the pancreas.
  • Ductal adenocarcinoma of the pancreas is one of the most common causes of cancer death. In the US, it causes approximately 30,000 deaths each year, and pancreatic cancer ranks 5th among cancer-related causes of death.
  • the diagnosis of pancreatic carcinoma in a patient represents an infertile prognosis.
  • the survival time of all patients with ductal pancreatic carcinoma is on average only 1 year, and only about 4% of the patients survive a period of 5 years.
  • the late diagnosis of these tumors is one reason for the poor survival rate.
  • pancreatic tumors The histological differential diagnosis of pancreatic tumors is often difficult. There are various antibodies used in the pathological routine (eg against cytokeratin 18), but with which it is not always possible to predict the course of the disease. In the investigation of dislocations (metastases) of various carcinomas of the pancreas, biliary tract and gallbladder, as well as the papilla vateri histological assignment to the primary tumor is usually not possible. An assignment of these metastases to the primary tumor is important for the selection of an adapted palliative chemotherapy.
  • pancreatic tumor marker For the early detection, the laboratory differential diagnosis of pancreatic tumors to other pancreatic diseases and the follow-up after surgery, there is so far only one marker, the CA19-9.
  • this tumor marker to be determined in the blood is not very specific and sensitive, i. there are patients with chronic pancreatitis (pancreatitis) who have a high Ca19-9 value and patients with normal Ca19-9 who have a pancreatic tumor.
  • ductal pancreatic cancer The treatment of ductal pancreatic cancer is currently limited to surgery and chemotherapy.
  • the surgical removal of the tumor offers the only option a chance of recovery.
  • Chemotherapy with the most commonly used chemotherapy Only in a few cases does a life improvement, but no cure. At present, only less than 40% of patients can be treated with curative treatment, and a large proportion of them die on average after 2 years.
  • the typical symptoms of end-stage pancreatic cancer are body wasting and severe pain. Both factors significantly affect the quality of life of patients.
  • pancreatic carcinoma adenocarcinoma .
  • K-ras, DPC4, p53 and p16 seem to play an important role in the development of pancreatic carcinoma (1-4).
  • microarray techniques have also been used to study tumor-specific gene expression in various types of cancers, i.a. also used in pancreatic carcinoma (5-20).
  • Previous studies of tumor-specific gene expression in pancreatic carcinoma were performed with whole tissue samples or with cell lines. In cell lines, however, changes in gene expression can be induced by in vitro conditions that are not present in vivo.
  • Tissue samples from the ductal adenocarcinoma of the pancreas therefore contain various cell types, such as ductal cells, acinar cells, islet cells, inflammatory and nerve cells, as well as fibrocystic elements.
  • the expression profile obtained may represent both the tumor tissue and the adjacent non-neoplastic tissue, making the data obtained questionable.
  • the object of the invention is to provide new methods for the diagnosis and prognosis as well as novel compounds for the treatment of pancreatic carcinoma, in particular of the ductal adenocarcinoma of the pancreas.
  • the object is achieved by a method for in vitro diagnosis of a pancreatic carcinoma, in particular of the ductal adenocarcinoma of the pancreas, wherein the amount of gene product of at least one of the genes listed in Table 1 and / or Table 2 in a sample of biological origin of an individual is determined , The amount of gene product in the sample of biological origin of an individual is compared to the amount of the corresponding gene product commonly present in normal pancreatic epithelial tissue. If it is a gene listed in Table 1, an increased amount of the gene product is an indication of the presence of a gene Pankreaskarzioms. If it is a gene listed in Table 2, a decreased amount of the gene product is an indication of the presence of a pancreatic carcinoma.
  • the invention is based on a study in which it was determined which genes in pancreatic carcinoma undergo an altered expression level compared to the normal pancreatic epithelial tissue. For this purpose, a microdissection of human pancreatic tissue and normal ductal pancreatic epithelial tissue was performed and then a comparative study of gene expression using the U 133 set of Affymetrix (Affymetrix Human Genome U133 Set, Santa Clara, Calif. CA, USA, Order Number: 900444) performed 45,000 Fragments corresponding to 33,000 known genes and 6,000 expressed sequence tags (ESTs).
  • Affymetrix Affymetrix Human Genome U133 Set, Santa Clara, Calif. CA, USA, Order Number: 900444
  • nucleic acid sequences and proteins were identified which undergo an altered expression level in pancreatic carcinoma compared to normal pancreatic epithelial tissue.
  • the altered expression of these genes is causally linked to the pancreatic tumor.
  • pancreatic tumor tissue samples used were homogeneous and do not contain non-neoplastic tissue. Due to the reduced heterogeneity of the microdissected tissue, even the smallest changes in gene expression can be observed.
  • the identification number of the Affymetrix sample set For characterization, the identification number of the Affymetrix sample set, the gene symbol, the gene name, the chromosomal localization, the representative identification number (ID), the identification number of the transcript and that of the corresponding protein are specified (publication numbers in the Entrez database of the National Center for Biotechnology Information (NCBI), Bethesda, USA (http://www.ncbi.nih.gov/entrez/)
  • the q value (in percent) indicates the statistical error with which the evaluation program used predicts differential gene expression.
  • PAK1 interacting protein 1 NM 017906 g8923576 NP 060376 Chr: 6p24.1 4.23644 3.612633
  • Nr Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-valve
  • Proteaseome (prosome, NMJ3Q2818 g4506236 NPJ302809 Chr: 14q11.2 2.31359 0.095261 'macropain) activator with 2 (PA28 beta) /// proteasome (prosome, macropain ⁇ activator subunit 2 (PA28 beta) ⁇ >
  • triosephosphate isomerase 1 NM 000365 g4507644 NP 000356 Chr: 12p13 2.12957 1.578435
  • conjuggase folylpoiygammaglutamyl hydrolase
  • gamma-glutamyl hydrolase conjuggase, folylpoiygammaglutamyl hydrolase
  • Table 2 lists genes whose expression in the ductal adenocarcinoma of the pancreas is on average at most half as high as the expression in normal pancreatic epithelial tissue. Ie. the fold change from the expression levels in the microdissected ductal adenocarcinoma of the pancreas (mean over 14 patients) and the expression values in the healthy microdissected ductal pancreas epithelium (mean over 11 persons) is ⁇ 0.5 for these genes the quotient ("fold change") indicated 1000, this means that the gene could be detected only in normal tissue, but not in tumor tissue. The genes are consecutively numbered from the number 541.
  • the identification number of the Affymetrix sample set For characterization, the identification number of the Affymetrix sample set, the gene symbol, the gene name, the chromosomal localization, the representative identification number (ID), the identification number of the transcript and that of the corresponding protein are specified (publication numbers in the Entrez database of the National Center for Biotechnology Information (NCBI), Bethesda, USA (http://www.ncbi.nih.gov/entrez/)
  • the q value (in percent) indicates the statistical error with which the evaluation program used predicts differential gene expression.
  • Oxidase, copper containing 3 vascular adhesion protein 1
  • beta A2 /// NM_005209 g7019356 NP_005200 Chr: 2q34-q36 0.41707 0.095261 crystailin, beta A2

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Abstract

The invention relates to methods for the diagnosis and prognosis of pancreatic carcinoma, particularly of ductal adenoma carcinoma of the pancreas, to methods for screening for active substances for treating pancreatic carcinoma, and to the use of antisense construction or siRNA molecules for producing a pharmaceutical composition for treating pancreatic carcinoma. By performing comparative examination of the gene expression in the pancreatic carcinoma and in the normal pancreatic epithelial tissue, all in all, 1419 differentially expressed genes (650 up-regulated and 769 down-regulated genes) were found. Until now, it was unknown that 1267 of the found 1419 genes were correlated with pancreatic tumors. It can be established whether a pancreatic carcinoma exists by determining the amount of gene product of a differentially expressed gene of this type in a sample of biological origin of an individual and by comparing it with the amount of gene product present in the pancreatic tissue.

Description

Verbindungen und Methoden zur Behandlung, Diagnose und Prognose bei PankreaserkrankungenCompounds and methods of treatment, diagnosis and prognosis in pancreatic diseases

Die Erfindung betrifft Nukleinsäuresequenzen sowie hierdurch kodierte Peptide bzw. Proteine, als auch die Verwendung von hieraus abgeleitenden Sequenzen zum Screenen nach daran bindenden Substanzen als auch die Verwendung von an den Nukleinsäuren und der daraus abgeleiteten Peptide bzw. Proteine bindenden Substanzen zur Diagnose, Prognose, sowie zur therapeutischen Behandlung bei Pankreastumoren, insbesondere beim duktalen Adenokarzinom des Pankreas.The invention relates to nucleic acid sequences and peptides or proteins encoded thereby, as well as the use of sequences derived therefrom for screening substances that bind thereto and the use of substances binding to the nucleic acids and peptides or proteins derived therefrom for diagnosis, prognosis, and for the therapeutic treatment of pancreatic tumors, in particular of the ductal adenocarcinoma of the pancreas.

Das duktale Adenokarzinom des Pankreas ist eine der häufigsten krebsbedingten Todesursachen. In den USA werden jährlich ca. 30 000 Todesfälle dadurch verursacht und das Pankreaskarzinom rangiert auf Platz 5 unter den krebsbedingten Todesursachen. Die Diagnose eines Pankreaskarzinoms bei einem Patienten stellt eine infauste Prognose dar. Die Überlebenszeit aller Patienten mit einem duktalen Pankreaskarzinom beträgt im Mittel nur 1 Jahr, und nur etwa 4% der Patienten überleben einen Zeitraum von 5 Jahren. Neben der speziellen Biologie des Pankreaskarzinoms ist auch die späte Diagnose dieser Tumore ein Grund für die schlechte Überlebensrate.Ductal adenocarcinoma of the pancreas is one of the most common causes of cancer death. In the US, it causes approximately 30,000 deaths each year, and pancreatic cancer ranks 5th among cancer-related causes of death. The diagnosis of pancreatic carcinoma in a patient represents an infertile prognosis. The survival time of all patients with ductal pancreatic carcinoma is on average only 1 year, and only about 4% of the patients survive a period of 5 years. In addition to the special biology of pancreatic carcinoma, the late diagnosis of these tumors is one reason for the poor survival rate.

Die histologische Differentialdiagnose von Pankreastumoren ist oft schwierig. Es existieren verschiedene in der pathologischen Routine angewendete Antikörper (z. B. gegen Zytokeratin 18), mit welchen aber nicht immer eine Prognose zum Krankheitsverlauf möglich ist. Bei der Untersuchung von Absiedelungen (Metastasen) von verschiedenen Karzinomen des Pankreas, der Gallenwege und der Gallenblase, wie auch der Papilla vateri ist meist die histologische Zuordnung zum Primärtumor nicht möglich. Eine Zuordnung dieser Metastasen zum Primärtumor ist zur Wahl einer angepassten palliativen Chemotherapie wichtig.The histological differential diagnosis of pancreatic tumors is often difficult. There are various antibodies used in the pathological routine (eg against cytokeratin 18), but with which it is not always possible to predict the course of the disease. In the investigation of dislocations (metastases) of various carcinomas of the pancreas, biliary tract and gallbladder, as well as the papilla vateri histological assignment to the primary tumor is usually not possible. An assignment of these metastases to the primary tumor is important for the selection of an adapted palliative chemotherapy.

Für die Früherkennung, die laborchemische Differentialdiagnose von Pankreastumoren zu anderen Bauchspeicheldrüsenerkrankungen und der Verlaufskontrolle nach Operation gibt es bisher nur einen Marker, das CA19-9. Dieser im Blut zu bestimmende Tumormarker ist allerdings nicht sehr spezifisch und sensitiv, d.h. es gibt Patienten mit chronischer Pankreatitis (Bauchspeicheldrüsenentzündung), welche einen stark erhöhten Ca19-9 Wert haben und Patienten mit normalem Ca19-9, welche einen Pankreastumor haben.For the early detection, the laboratory differential diagnosis of pancreatic tumors to other pancreatic diseases and the follow-up after surgery, there is so far only one marker, the CA19-9. However, this tumor marker to be determined in the blood is not very specific and sensitive, i. there are patients with chronic pancreatitis (pancreatitis) who have a high Ca19-9 value and patients with normal Ca19-9 who have a pancreatic tumor.

Die Behandlung des duktalen Pankreaskarzinoms ist derzeit auf die Operation und Chemotherapie begrenzt. Die operative Entferung des Tumors bietet als einzige Option eine Heilungschance. Die Chemotherapie mit dem meist eingesetzten Chemotherapeutikum bietet nur in wenigen Fällen eine Lebensverbesserung, aber keine Heilung. Es können zur Zeit nur weniger als 40% der Patienten kurativ behandelt werden und von diesen stirbt ein großer Teil im Durchschnitt nach 2 Jahren. Die typischen Symptome im Endstadium des Pankreaskarzinoms sind eine Auszehrung des Körpers und starke Schmerzen. Beide Faktoren beeinträchtigen die Lebensqualtität der Patienten erheblich.The treatment of ductal pancreatic cancer is currently limited to surgery and chemotherapy. The surgical removal of the tumor offers the only option a chance of recovery. Chemotherapy with the most commonly used chemotherapy Only in a few cases does a life improvement, but no cure. At present, only less than 40% of patients can be treated with curative treatment, and a large proportion of them die on average after 2 years. The typical symptoms of end-stage pancreatic cancer are body wasting and severe pain. Both factors significantly affect the quality of life of patients.

In den letzten Jahren wurden einige mit der Krebsentstehung in Verbindung stehende Gene im duktalen Adenokarzinom des Pankreas identifiziert. K-ras, DPC4, p53 und p16 scheinen dabei eine wichtige Rolle bei der Entstehung des Pankreaskarzinoms zu spielen (1-4). Seit kurzem werden auch Microarray-Techniken zur Untersuchung der tumorspezifischen Genexpression bei verschiedenen Krebsarten, u.a. auch beim Pankreaskarzinom, angewendet (5-20). Bisherige Untersuchungen der tumorspezifischen Genexpression beim Pankreaskarzinom wurden mit ganzen Gewebeproben oder mit Zelllinien durchgeführt. In Zelllinien können aber durch die Bedingungen in vitro Veränderungen in der Genexpression induziert werden, die so in vivo nicht vorliegen.In recent years, several genes associated with carcinogenesis have been identified in pancreatic ductal adenocarcinoma. K-ras, DPC4, p53 and p16 seem to play an important role in the development of pancreatic carcinoma (1-4). Recently, microarray techniques have also been used to study tumor-specific gene expression in various types of cancers, i.a. also used in pancreatic carcinoma (5-20). Previous studies of tumor-specific gene expression in pancreatic carcinoma were performed with whole tissue samples or with cell lines. In cell lines, however, changes in gene expression can be induced by in vitro conditions that are not present in vivo.

Sowohl der Tumor als auch das Pankreasnormalgewebe verfügen über eine heterogene Struktur. Gewebeproben aus dem duktalen Adenokarzinom des Pankreas enthalten daher verschiedene Zelltypen, wie duktale Zellen, azinöse Zellen, Inselzellen, Entzündungs- und Nervenzellen, sowie fibrozystische Elemente. Wenn ganze Gewebeproben in Microarray- Untersuchungen eingesetzt werden, kann das erhaltene Expressionsprofil sowohl das Tumorgewebe als auch das benachbarte nicht-neoplastische Gewebe repräsentieren, was die erhaltenden Daten fragwürdig erscheinen läßt.Both the tumor and normal pancreatic tissue have a heterogeneous structure. Tissue samples from the ductal adenocarcinoma of the pancreas therefore contain various cell types, such as ductal cells, acinar cells, islet cells, inflammatory and nerve cells, as well as fibrocystic elements. When entire tissue samples are used in microarray assays, the expression profile obtained may represent both the tumor tissue and the adjacent non-neoplastic tissue, making the data obtained questionable.

Aufgabe der Erfindung ist es, neue Verfahren für die Diagnose und Prognose sowie neue Verbindungen für die Behandlung des Pankreaskarzinoms, insbesondere des duktalen Adenokarzinoms des Pankreas, bereitzustellen.The object of the invention is to provide new methods for the diagnosis and prognosis as well as novel compounds for the treatment of pancreatic carcinoma, in particular of the ductal adenocarcinoma of the pancreas.

Erfindungsgemäß wird die Aufgabe gelöst durch ein Verfahren zur in vitro Diagnose eines Pankreaskarzinoms, insbesondere des duktalen Adenokarzinoms des Pankreas, wobei die Menge an Genprodukt von mindestens einem der in Tabelle 1 und/oder Tabelle 2 aufgeführten Gene in einer Probe biologischen Ursprungs eines Individuums bestimmt wird. Die Menge an Genprodukt in der Probe biologischen Ursprungs eines Individuums wird mit der Menge des entsprechenden Genprodukts, die in normalem Pankreasepithelgewebe üblicherweise vorliegt, verglichen. Falls es sich um ein in der Tabelle 1 aufgeführtes Gen handelt, ist eine erhöhte Menge des Genprodukts ein Indiz für das Vorliegen eines Pankreaskarzioms. Falls es sich um ein in der Tabelle 2 aufgeführtes Gen handelt, ist eine erniedrigte Menge des Genprodukts ein Indiz für das Vorliegen eines Pankreaskarzinoms.According to the invention, the object is achieved by a method for in vitro diagnosis of a pancreatic carcinoma, in particular of the ductal adenocarcinoma of the pancreas, wherein the amount of gene product of at least one of the genes listed in Table 1 and / or Table 2 in a sample of biological origin of an individual is determined , The amount of gene product in the sample of biological origin of an individual is compared to the amount of the corresponding gene product commonly present in normal pancreatic epithelial tissue. If it is a gene listed in Table 1, an increased amount of the gene product is an indication of the presence of a gene Pankreaskarzioms. If it is a gene listed in Table 2, a decreased amount of the gene product is an indication of the presence of a pancreatic carcinoma.

Der Erfindung liegt eine Untersuchung zugrunde, in der ermittelt wurde, welche Gene im Pankreaskarzinom eine veränderte Expressionshöhe gegenüber dem normalen Pankreasephithelgewebe erfahren. Dazu wurde eine Mikrodissektion von humanem Pankreastumorgewebe und von normalem duktalem Pankreasepithelgewebe durchgeführt und anschließend eine vergleichende Untersuchung der Genexpression mittels des U 133 Sets von Affymetrix (Affymetrix Human Genome U133 Set, Santa Clara, CA, USA, Bestellnummer: 900444) durchgeführt, das 45 000 Fragmente, die zu 33 000 bekannten Genen und 6 000 expressed sequence tags (ESTs) korrespondieren, enthält.The invention is based on a study in which it was determined which genes in pancreatic carcinoma undergo an altered expression level compared to the normal pancreatic epithelial tissue. For this purpose, a microdissection of human pancreatic tissue and normal ductal pancreatic epithelial tissue was performed and then a comparative study of gene expression using the U 133 set of Affymetrix (Affymetrix Human Genome U133 Set, Santa Clara, Calif. CA, USA, Order Number: 900444) performed 45,000 Fragments corresponding to 33,000 known genes and 6,000 expressed sequence tags (ESTs).

Auf diese Weise wurden Nukleinsäuresequenzen und Proteine identifiziert, die im Pankreaskarzinom eine veränderte Expressionshöhe gegenüber dem normalen Pankreasepithelgewebe erfahren. Die veränderte Expression dieser Gene ist ursächlich mit dem Pankreastumor verbunden.In this way, nucleic acid sequences and proteins were identified which undergo an altered expression level in pancreatic carcinoma compared to normal pancreatic epithelial tissue. The altered expression of these genes is causally linked to the pancreatic tumor.

Bei der der Erfindung zugrunde liegenden wissenschaftlichen Untersuchung wurde das Verfahren der Mikrodissektion zur Probengewinnung angewandt, um sicherzustellen, dass die verwendeten Pankreastumorgewebeproben homogen sind und kein nicht-neoplastisches Gewebe enthalten. Aufgrund der reduzierten Heterogenität des mikrodissektierten Gewebes können auch kleinste Veränderungen in der Genexpression beobachtet werden.In the scientific study underlying the invention, the method of microdissection for sample collection was used to ensure that the pancreatic tumor tissue samples used are homogeneous and do not contain non-neoplastic tissue. Due to the reduced heterogeneity of the microdissected tissue, even the smallest changes in gene expression can be observed.

Zudem wurde in den bisherigen Untersuchungen nur ein kleiner Teil des menschlichen Transkriptoms untersucht. Damit war es bisher nicht möglich, alle Gene, deren Expression beim duktalen Adenokarzinom des Pankreas verändert ist, zu identifizieren. Bei der zugrundelegenden Untersuchung mit dem Affymetrix U 133 Chip wurde hingegen das komplette Genom untersucht.In addition, only a small part of the human transcriptome has been investigated in the previous investigations. Thus, it has not been possible to identify all genes whose expression is altered in the ductal adenocarcinoma of the pancreas. In the underlying investigation with the Affymetrix U 133 chip, however, the complete genome was examined.

Beim Vergleich der erhaltenen Genexpressionsprofile wurden insgesamt 1419 differentiell exprimierte Gene gefunden (650 hochregulierte und 769 herunterregulierte Gene). Überraschenderweise war nur ein Bruchteil dieser Gene bereits zuvor von anderen Gruppen als mit Pankreastumor in Verbindung stehend beschrieben worden. Von 1267 der gefundenen 1419 Gene war hingegen nicht bekannt, dass sie im Zusammenhang mit Pankreastumor stehen. In Tabelle 1 sind Gene aufgeführt, deren Expression im duktalen Adenokarzinom des Pankreas im Mittel mindestens doppelt so hoch ist wie die Expression in normalem Pankreasepithelgewebe. D. h. der Quotient („Fold Change") aus den Expressionswerten im mikrodissektierten duktalen Adenomakarzinom des Pankreas (Mittel über 14 Patienten) und den Expressionswerten im gesunden mikrodissektierten duktalen Pankreasepithelgewebe (Mittel über 11 Personen) ist für diese Gene > 2. Wird für ein Gen der Quotient („Fold Change") 1000 angegeben, bedeutet dies, dass das Gen nur in dem Tumor-, aber nicht in dem Normalgewebe nachgewiesen werden konnte. Die Gene sind fortlaufend nummeriert. Zur Charakterisierung werden jeweils die Identifikationsnummer des Affymetrix-Probensets, das Gensymbol, der Gen-Name, die chromosomale Lokalisation, die repräsentative Identifikationsnummer (ID), die Identifikationsnummer des Transkriptes und die des zugehörigen Proteins angegeben (Veröffentlichungsnummern in der Entrez-Datenbank des National Center for Biotechnology Information (NCBI), Bethesda, USA (http://www.ncbi.nih.gov/entrez/). Der q-Wert (in Prozent) gibt an, mit welchem statistischen Fehler das verwendete Auswerteprogramm eine differentielle Genexpression voraussagt. When comparing the gene expression profiles obtained, a total of 1419 differentially expressed genes were found (650 upregulated and 769 downregulated genes). Surprisingly, only a fraction of these genes had previously been described by groups other than pancreatic tumors. Of 1267 of the 1419 genes found, however, it was not known that they are related to pancreatic tumors. Table 1 lists genes whose expression in the ductal adenocarcinoma of the pancreas is on average at least twice as high as the expression in normal pancreatic epithelial tissue. Ie. the quotient (fold change) from the expression values in the microdissected ductal adenoma carcinoma of the pancreas (mean over 14 patients) and the expression values in the healthy microdissected ductal pancreas epithelium (mean over 11 persons) is> 2 for these genes ("Fold change") indicated 1000, this means that the gene could be detected only in the tumor, but not in the normal tissue. The genes are numbered consecutively. For characterization, the identification number of the Affymetrix sample set, the gene symbol, the gene name, the chromosomal localization, the representative identification number (ID), the identification number of the transcript and that of the corresponding protein are specified (publication numbers in the Entrez database of the National Center for Biotechnology Information (NCBI), Bethesda, USA (http://www.ncbi.nih.gov/entrez/) The q value (in percent) indicates the statistical error with which the evaluation program used predicts differential gene expression.

Tabelle 1:Table 1:

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID ProteinJD Lokalisierung Change /0/-Sample Number ID ProteinJD Localization Change / 0 / -

1 243329 at Clone 1MAGE:121662 mRNA A1074450 Hs.18106.0 1000 9.266777 sequence1 243329 at Clone 1MAGE: 121662 mRNA A1074450 Hs.18106.0 1000 9.266777 sequence

2 236621 at Transcribed sequence with AV683684 Hs.40838.0 — 1000 strong similarity to protein pir:A48045 (H.sapiens) A48045 ribosomal protein S27, cytosolic - humanHs40838.0 - 1000 strong similarity to protein: A48045 (H.sapiens) A48045 ribosomal protein S27, cytosolic - human

3 230619_at ARNT aryl hydrocarbon receptor AI768497 Hs.166172.2 NP_001659 Chr:1q21 1000 13.5285 nuciear translocator3 230619_at ARNT aryl hydrocarbon receptor AI768497 Hs.166172.2 NP_001659 Chr: 1q21 1000 13.5285 nuclear translocator

4 228505 s at LOC124491 LOC124491 N49836 Hs.236456.0 NP 660297 Chr:16q22.3 1000 9.2667774 228505 s at LOC124491 LOC124491 N49836 Hs.236456.0 NP 660297 Chr: 16q22.3 1000 9.266777

5 227227 at AI344332 Hs.190488.4 1000 9.2667775 227227 at AI344332 Hs.190488.4 1000 9.266777

6 222191_s_at B4GALT7 xylosylprotein beta 1,4- AK022566 Hs.54702.1 NP_009186 Chr:5q35.2- 1000 galactosyltransferase, q35.3 Polypeptide 7 (galactosyltransferase I)6 222191_s_at B4GALT7 xylosyl protein beta 1,4-AK022566 Hs.54702.1 NP_009186 Chr: 5q35.2-1000 galactosyltransferase, q35.3 polypeptides 7 (galactosyltransferase I)

7 221352_at HUMBINDC DNA binding protein for NM_013288 g7019420 1000 12.53374 °" Surfactant protein B /// DNA binding protein for Surfactant protein B7 221352_at HUMBINDC DNA binding protein for NM_013288 g7019420 1000 12.53374 ° "Surfactant protein B /// DNA binding protein for surfactant protein B

8 219510_at POLQ Polymerase (DNA directed), NM_006596 g5729983 NP_006587 Chr:3q13.33 1000 theta /// Polymerase (DNA directed), theta8 219510_at POLQ Polymerase (DNA directed), NM_006596 g5729983 NP_006587 Chr: 3q13.33 1000 theta /// Polymerase (DNA directed), theta

9 219182_at CHST5 carbohydrate (N- NM_024533 gl 3375685 NP_036258 Chr:16q22.3 1000 1.578435 acetylglucosamine 6-O) sulfotransferase 59 219182_at CHST5 carbohydrates (N-NM_024533 gl 3375685 NP_036258 Chr: 16q22.3 1000 1.578435 acetylglucosamine 6-O) sulfotransferase 5

10 218663_at HCAP-G chromosome condensation NM_022346 gl 1641252 NP_071741 Chr:4p16-p15 1000 4.149813 protein G10 218663_at HCAP-G chromosome condensation NM_022346 gl 1641252 NP_071741 Chr: 4p16-p15 1000 4.149813 protein G

11 218355_at KIF4A kinesin family member 4A /// NM_012310 g7305204 NPJD36442 Chr:xq13.1 1000 3.612633 kinesin family member 4A11 218355_at KIF4A kinesin family member 4A /// NM_012310 g7305204 NPJD36442 Chr: xq13.1 1000 3.612633 kinesin family member 4A

12 217770_at PlGT phosphatidylinositol glycan, NM_015937 g7706257 NPJD57021 Chr:20q12- 1000 class T /// phosphatidylinositol q13.12 giycan, class T12 217770_at PlGT phosphatidylinositol glycan, NM_015937 g7706257 NPJD57021 Chr: 20q12- 1000 class T /// phosphatidylinositol q13.12 giycan, class T

13 217466_x_at RPS2 ribosomal protein S2 L48784 Hs.182426.4 NP_002943 Chr:16p13.3 1000 13 217466_x_at RPS2 ribosomal protein S2 L48784 Hs.182426.4 NP_002943 Chr: 16p13.3 1000

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

14 213599 at OIP5 Opa-interacting protein 5 BE045993 Hs.116206.0 NP 009211 Chr:15q14 1000 12.5337414 213599 at OIP5 Opa-interacting protein 5 BE045993 Hs.116206.0 NP 009211 Chr: 15q14 1000 12.53374

15 212680 x at LIMK2 LIM domain kinase 2 BE305165 Hs.100623.2 NP 005560 Chr:22q12.2 1000 6.99135815 212680 x at LIMK2 LIM domain kinase 2 BE305165 Hs.100623.2 NP 005560 Chr: 22q12.2 1000 6.991358

16 212376 s at EP400 E1A binding protein p400 BE880591 Hs.306094.0 NP_056224 Chr:12q24.33 1000 13.4032916 212376 s at EP400 E1A binding protein p400 BE880591 Hs.306094.0 NP_056224 Chr: 12q24.33 1000 13.40329

17 211585_at NPAT nuclear protein, ataxia- U58852 gl 381666 NP_002510 Chr:11q22-q23 1000 13.40329 telangiectasia locus17 211585_at NPAT nuclear protein, ataxia U58852 gl 381666 NP_002510 Chr: 11q22-q23 1000 13.40329 telangiectasia locus

18 208803_s_at SRP72 Signal recognition particle AF069765 g3243032 NP_008878 Chr:4q11 1000 6.991358 72kDa18 208803_s_at SRP72 Signal recognition particle AF069765 g3243032 NP_008878 Chr: 4q11 1000 6.991358 72kDa

19 208369_s_at GCDH glutaryl-Coenzyme A NM_013976 g7669493 NP_000150 Chr:19p13.2 1000 dehydrogenase /// glutaryl- Coenzyme A dehydrogenase19 208369_s_at GCDH glutaryl coenzymes A NM_013976 g7669493 NP_000150 Chr: 19p13.2 1000 dehydrogenase /// glutaryl-coenzyme A dehydrogenase

20 206558_at SIM2 single-minded homolog 2 NM_005069 g7108363 NP_005060 Chr:21q22.13 1000 (Drosophila) /// single-minded homolog 2 (Drosophila)20 206558_at SIM2 single-minded homologue 2 NM_005069 g7108363 NP_005060 Chr: 21q22.13 1000 (Drosophila) /// single-minded homolog 2 (Drosophila)

21 206043 s at KIAA0703 KIAA0703 gene product NM 014861 g7662255 — Chr:16q24.1 100021 206043 s at KIAA0703 KIAA0703 gene product NM 014861 g7662255 - Chr: 16q24.1 1000

22 205653 at CTSG cathepsin G /// cathepsin G NM 001911 g4503148 NP 001902 Chr:14q11.2 1000 1.57843522 205653 at CTSG cathepsin G /// cathepsin G NM 001911 g4503148 NP 001902 Chr: 14q11.2 1000 1.578435

23 204948 s at FST follistatin NM 013409 g7242221 NP 006341 Chr:5q11.2 1000 3.61263323 204948 s at FST follistatin NM 013409 g7242221 NP 006341 Chr: 5q11.2 1000 3.612633

24 203870 at USP46 ubiquitin specific protease 46 BE856374 Hs.109268.0 NP 073743 Chr:4q12 1000 4.436744 c

Figure imgf000007_0001
\24 203870 at USP46 ubiquitin specific protease 46 BE856374 Hs.109268.0 NP 073743 Chr: 4q12 1000 4.436744 c
Figure imgf000007_0001
\

25 203596_s_at IFIT5 interferon-induced protein with NM_012420 g6912629 NP_036552 Chr:10q23.32 1000 4.830969 tetratricopeptide repeats 525 203596_s_at IFIT5 interferon-induced protein with NM_012420 g6912629 NP_036552 Chr: 10q23.32 1000 4.830969 tetratricopeptide repeats 5

26 203580_s_at SLC7A6 solute carrier family 7 (cationic NM_003983 g4507052 Chr:16q22.1 1000 8.779357 amino acid transporter, y+ System), member 626 203580_s_at SLC7A6 solute carrier family 7 (cationic NM_003983 g4507052 Chr: 16q22.1 1000 8.779357 amino acid transporter, y + system), member 6

27 203233_at IL4R interleukin 4 receptor /// NM_000418 g4557668 NP_000409 Chr:16p11.2- 1000 interleukin 4 receptor 12.127 203233_at IL4R interleukin 4 receptor /// NM_000418 g4557668 NP_000409 Chr: 16p11.2- 1000 interleukin 4 receptor 12.1

28 203089 s at PRSS25 Protease, serine, 25 NM_013247 g7019476 NP_037379 Chr:2p12 100028 203089 s at PRSS25 Protease, serine, 25 NM_013247 g7019476 NP_037379 Chr: 2p12 1000

29 203035_s_at PIAS3 protein inhibitor of activated NM_006099 g5174628 NP_006090 Chr:1q21 1000 8.153992 STAT3 /// protein inhibitor of activated STAT329 203035_s_at PIAS3 protein inhibitor of activated NM_006099 g5174628 NP_006090 Chr: 1q21 1000 8.153992 STAT3 /// protein inhibitor of activated STAT3

30 91826 at EPS8L1 EPS8-like 1 AI219073 4919761 RC NP 060199 Chr:19q13.42 1000 1.70287730 91826 at EPS8L1 EPS8-like 1 AI219073 4919761 RC NP 060199 Chr: 19q13.42 1000 1.702877

31 34063_at RECQL5 RecQ protein-like 5 AB006533 4822898 NPJD04250 Chr:17q25.2- 1000 2.08989 q25.331 34063_at RECQL5 RecQ protein-like 5 AB006533 4822898 NPJD04250 Chr: 17q25.2- 1000 2.08989 q25.3

32 243670_at — Transcribed sequences BE670161 Hs.253652.0 — — 1000 1.901012 32 243670_at - Transcribed sequences BE670161 Hs.253652.0 - - 1000 1.901012

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change ( o7/o.Sample ID ID Protein ID Localization Change (o7 / o.

33 243075 at Transcribed sequence with AW292940 Hs.290115.0 1000 1.051298 moderate similarity to protein sp:P39194 (H.sapiens) ALU7_HUMAN AIu subfamily SQ sequence contamination warning entryHs.290115.0 1000 1.051298 moderate similarity to protein sp: P39194 (H.sapiens) ALU7_HUMAN AIu subfamily SQ sequence contamination warning entry

34 239733_at MGC16186 hypothetical protein MGC16186 AI185136 Hs.48650.0 NP 115748 Chr:10q23.1 1000 1.70287734 239733_at MGC16186 hypothetical protein MGC16186 AI185136 Hs.48650.0 NP 115748 Chr: 10q23.1 1000 1.702877

35 236174_at — Transcribed sequences A1808830 Hs.43938.0 — — 1000 0.51816735 236174_at - Transcribed sequences A1808830 Hs.43938.0 - - 1000 0.518167

36 235548_at FLJ90166 hypothetical protein FLJ90166 BG326592 Hs.119286.0 NP 699191 Chr:20q13.32 1000 0.149911FLU90166 BG326592 Hs.119286.0 NP 699191 Chr: 20q13.32 1000 0.149911

37 232897 at FLJ20444 hypothetical protein FLJ20444 AK000451 Hs.285336.0 NP 982274 Chr:9q21 1000 0.14991137 232897 at FLJ20444 hypothetical protein FLJ20444 AK000451 Hs.285336.0 NP 982274 Chr: 9q21 1000 0.149911

38 231286_at EDG3 endothelial differentiation, AW452080 Hs.160623.0 NP_005217 Chr:9q22.1- 1000 1.380569 sphingolipid G-protein-coupled q22.2 receptor, 338 231286_at EDG3 endothelial differentiation, AW452080 Hs.160623.0 NP_005217 Chr: 9q22.1-1000 1.380569 sphingolipid G protein-coupled q22.2 receptor, 3

39 229608_at LOC284465 hypothetical protein BF246946 Hs.26000.0 — Chr:1 p13.2 1000 1.051298 LOC28446539 229608_at LOC284465 hypothetical protein BF246946 Hs.26000.0 - Chr: 1 p13.2 1000 1.051298 LOC284465

40 229389 at — Transcribed sequences AA741058 Hs.20065.0 — — 1000 2.0898940 229389 at - Transcribed sequences AA741058 Hs.20065.0 - - 1000 2.08989

41 228558_at C14orf80 chromosome 14 open reading AL518291 Hs.72363.0 NP_775879 Chr:14q32.33 1000 3.62324 frame 8041 228558_at C14orf80 chromosome 14 open reading AL518291 Hs.72363.0 NP_775879 Chr: 14q32.33 1000 3.62324 frame 80

42 223719 s at RTBDN retbindin BC005063 gl 3477198 NP 113617 Chr:19p12 1000 0.09526142 223719 s at RTBDN retbindin BC005063 gl 3477198 NP 113617 Chr: 19p12 1000 0.095261

43 223642_at ZIC2 Zic family member 2 (odd- AF193855 gl 1065969 NP_009060 Chr:13q32 1000 3.306454 paired homolog, Drosophila)43 223642_at ZIC2 Zic family member 2 (odd AF193855 gl 1065969 NP_009060 Chr: 13q32 1000 3.306454 paired homolog, Drosophila)

44 223389 s at ZNF581 zinc finger protein 581 AF151023 g7239106 NP 057619 Chr:19q 13.43 1000 2.48911744 223389 s at ZNF581 zinc finger protein 581 AF151023 g7239106 NP 057619 Chr: 19q 13.43 1000 2.489117

45 222083_at GLYAT glycine-N-acyltransferase AW024233 Hs.18508.1 NP 005829 Chr:11q12.2 1000 0.35366945 222083_at GLYAT glycine N-acyltransferase AW024233 Hs.18508.1 NP 005829 Chr: 11q12.2 1000 0.353669

46 218695_at EXOSC4 exosome component 4 NM 019037 g9506688 NP 061910 Chr:8q24.3 1000 4.17418946 218695_at EXOSC4 exosome component 4 NM 019037 g9506688 NP 061910 Chr: 8q24.3 1000 4.174189

47 214478_at SPP2 secreted phosphoprotein 2, NM_006944 Hs.12230.0 NP_008875 Chr:2q37-qter 1000 1.901012 24kDa /// secreted phosphoprotein 2, 24kDa47 214478_at SPP2 secreted phosphoprotein 2, NM_006944 Hs.12230.0 NP_008875 Chr: 2q37-qter 1000 1.901012 24kDa /// secreted phosphoprotein 2, 24kDa

48 213559_s_at EZI likely ortholog of mouse zinc BF223401 Hs.112158.0 NP_997219 Chr:7q36.1 1000 1.051298 finger protein EZI48 213559_s_at EZI likely orthologue of mouse zinc BF223401 Hs.112158.0 NP_997219 Chr: 7q36.1 1000 1.051298 finger protein EZI

49 213201_s_at TNNT1 troponin T1, skeletal, slow AJ011712 Hs.73980.1 NP 003274 Chr:19q13.4 1000 2.48911749 213201_s_at TNNT1 troponin T1, skeletal, slow AJ011712 Hs.73980.1 NP 003274 Chr: 19q13.4 1000 2.489117

50 213013_at MAPK8IP1 mitogen-activated protein BG164295 Hs.234249.0 NPJD05447 Chr:11p12- 1000 0.095261 kinase 8 interacting protein 1 p11.250 213013_at MAPK8IP1 mitogen-activated protein BG164295 Hs.234249.0 NPJD05447 Chr: 11p12- 1000 0.095261 kinase 8 interacting protein 1 p11.2

51 212318 at TNPO3 transportin 3 /// transportin 3 NM 012470 Hs.69235.0 NP_036602 Chr:7q32.3 1000 0.625663 51 212318 at TNPO3 transportin 3 /// transportin 3 NM 012470 Hs.69235.0 NP_036602 Chr: 7q32.3 1000 0.625663

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change ( /o)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (/ o)

52 208097_s_at TXNDC thioredoxin domain containing NM_030755 gl 3559515 NPJ 10382 Chr:14q22.1 1000 0.095261 /// thioredoxin domain containing /// thioredoxin domain containing52 208097_s_at TXNDC thioredoxin domain containing NM_030755 gl 3559515 NPJ 10382 Chr: 14q22.1 1000 0.095261 /// thioredoxin domain containing /// thioredoxin domain containing

53 207980_s_at CITED2 Cbp/p300-interacting NM_006079 g5174416 NP_006070 Chr.6q23.3 1000 0.095261 transactivator, with Glu/Asp-rich carboxy-terminal domain, 253 207980_s_at CITED2 Cbp / p300-interacting NM_006079 g5174416 NP_006070 Chr.6q23.3 1000 0.095261 transactivator, with Glu / Asp-rich carboxy-terminal domain, 2

54 207322_at ITSN1 intersectin 1 (SH3 domain NM_003024 g4504796 NP_001001132 Chr:21q22.1- 1000 2.489117 protein) q22.254 207322_at ITSN1 intersectin 1 (SH3 domain NM_003024 g4504796 NP_001001132 Chr: 21q22.1-1000 2.489117 protein) q22.2

55 206899_at NTSR2 neurotensin receptor 2 /// NM_012344 g6912537 NP_036476 Chr:2p25.1 1000 0.518167 neurotensin receptor 255 206899_at NTSR2 neurotensin receptor 2 /// NM_012344 g6912537 NP_036476 Chr: 2p25.1 1000 0.518167 neurotensin receptor 2

56 206696_at GPR143 G protein-coupled receptor 143 NM_000273 g4557806 NPJD00264 Chr:xp22.3 1000 0.095261 /// G protein-coupled receptor 14356 206696_at GPR143 G protein-coupled receptor 143 NM_000273 g4557806 NPJD00264 Chr: xp22.3 1000 0.095261 /// G protein-coupled receptor 143

57 204832_s_at BMPR1A bone morphogenetic protein NM_004329 g4757853 NP_004320 Chr:10q22.3 1000 0.095261 receptor, type IA57 204832_s_at BMPR1A bone morphogenetic protein NM_004329 g4757853 NP_004320 Chr: 10q22.3 1000 0.095261 receptor, type IA

58 204656_at MCART1 mitochondrial carrier triple AL138752 Hs.244542.0 NP_219480 Chr:9p13.3-p12 1000 1.702877 repeat 158 204656_at MCART1 mitochondrial carrier triple AL138752 Hs.244542.0 NP_219480 Chr: 9p13.3-p12 1000 1.702877 repeat 1

59 202937_x_at CGI-96 CGI-96 protein AL022316 Hs.239934.0 NP_056518 Chr:22q13.2- 1000 0.625663 q13.3159 202937_x_at CGI-96 CGI-96 protein AL022316 Hs.239934.0 NP_056518 Chr: 22q13.2- 1000 0.625663 q13.31

60 201459_at RUVBL2 RuvB-like 2 (E. coli) /// RuvB- NM_006666 g5730022 NPJ)06657 Chr:19q13.3 1000 1.901012 like 2 (E. coli)60 201459_at RUVBL2 RuvB-like 2 (E. coli) /// RuvB- NM_006666 g5730022 NPJ) 06657 Chr: 19q13.3 1000 1.901012 like 2 (E. coli)

61 207301 at EFNA5 ephrin-A5 /// ephrin-A5 NM_001962 g4503486 NP 001953 Chr:5q21 44.02956 0.35366961 207301 at EFNA5 ephrin-A5 /// ephrin-A5 NM_001962 g4503486 NP 001953 Chr: 5q21 44.02956 0.353669

62 221579_s_at NUDT3 nudix (nucleoside diphosphate AF062530 g3978225 NP_006694 Chr:6p21.2 . 27.3708 0.353669 linked moiety X)-type motif 3 /// nudix (nucleoside diphosphate linked moiety X)-type motif 362 221579_s_at NUDT3 nudix (nucleoside diphosphate AF062530 g3978225 NP_006694 Chr: 6p21.2. 27.3708 0.353669 linked moiety X) -type motif 3 /// nudix (nucleoside diphosphate linked moiety X) -type motif 3

63 237923 at — Transcribed sequences AI733801 Hs.146186.0 — — 26.281 2.48911763 237923 at - Transcribed sequences AI733801 Hs.146186.0 - - 26.281 2.489117

64 237411 at LOC345667 similar to ADAMTS-10 N71063 Hs.38173.0 NP_922932 Chr:5q12.3 25.15901 0.518167 precursor (A disintegrin and metalloproteinase with thrombospondin motifs 10) (ADAM-TS 10) (ADAM-TS10)ADAMTS-10 N71063 Hs.38173.0 NP_922932 Chr: 5q12.3 25.15901 0.518167 precursor (A disintegrin and metalloproteinase with thrombospondin motifs 10) (ADAM-TS 10) (ADAM-TS10)

65 222854_s_at FLJ20514 hypothetical protein FLJ20514 AA044921 Hs.44423.0 NP_060326 Chr:xp22.31 20.84851 3.6232465 222854_s_at FLJ20514 hypothetical protein FLJ20514 AA044921 Hs.44423.0 NP_060326 Chr: xp22.31 20.84851 3.62324

66 223229 at HSPC150 HSPC150 protein similar to AB032931 g7416119 NP_054895 Chr.iq32.1 18.17979 0.201325 ubiquitin-conjugating eπzyme 66 223229 at HSPC150 HSPC150 protein similar to AB032931 g7416119 NP_054895 Chr.iq32.1 18.17979 0.201325 ubiquitin-conjugating eπzyme

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung ChangeNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change

67 218741_at C22orfl8 chromosome 22 open reading NM_024053 gl 3129021 NP_076958 Chr:22q13.31 18.14555 1.578435 frame 1867 218741_at C22orfl8 chromosomes 22 open reading NM_024053 gl 3129021 NP_076958 Chr: 22q13.31 18.14555 1.578435 frame 18

68 213561_at ASF1A ASF1 anti-silencing function 1 BF062335 Hs.108110.1 NP_054753 Chr:6q22.31 12.20258 2.08989 homolog A (S. cerevisiae)68 213561_at ASF1A ASF1 anti-silencing function 1 BF062335 Hs.108110.1 NP_054753 Chr: 6q22.31 12.20258 2.08989 homolog A (S. cerevisiae)

69 205278_at GAD1 glutamate decarboxylase 1 NM_0QQ817 g4503872 NP_000808 Chr:2q31 10.53315 4.640066 (brain, 67kDa) /// glutamate decarboxylase 1 {brain, 67kDa)69 205278_at GAD1 glutamate decarboxylase 1 NM_0QQ817 g4503872 NP_000808 Chr: 2q31 10.53315 4.640066 (brain, 67kDa) /// glutamate decarboxylase 1 {brain, 67kDa)

70 223274_at TCF19 traπscription factor 19 (SC1) BC002493 gl 2803348 NP 009040 Chr:6p21.3 10.3174 1.38056970 223274_at TCF19 trapping factor 19 (SC1) BC002493 gl 2803348 NP 009040 Chr: 6p21.3 10.3174 1.380569

71 214035_x_at LOC339047 hypothetica! protein AA308853 Hs.251928.2 — Chr:16p13.12 9.98884 1.901012 LOC33904771 214035_x_at LOC339047 hypothetica! protein AA308853 Hs.251928.2 - Chr: 16p13.12 9.98884 1.901012 LOC339047

72 219650 at FLJ20105 hypothetical protein FLJ20105 NM 017669 g8923111 — Chr:xq13.1 9.23987 2.81491972 219650 at FLJ20105 hypothetical protein FLJ20105 NM 017669 g8923111 - Chr: xq13.1 9.23987 2.814919

73 204032_at BCAR3 breast cancer anti-estrogen NM_003567 g4502370 NPJD03558 Chr:1p22.1 9.08488 6.225758 resistance 3 /// breast cancer anti-estrogen resistance 373 204032_at BCAR3 breast cancer anti-estrogen NM_003567 g4502370 NPJD03558 Chr: 1p22.1 9.08488 6.225758 resistance 3 /// breast cancer anti-estrogen resistance 3

74 202833_s_at SERPINA1 serine (or cysteine) Proteinase NMJ300295 g4505792 NP_000286 Chr:14q32.1 9.07322 10.20301 inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 174 202833_s_at SERPINA1 serine (or cysteine) proteinase NMJ300295 g4505792 NP_000286 Chr: 14q32.1 9.07322 10.20301 inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1

75 219257_s_at SPHK1 sphingosine kinase 1 /// NMJ)21972 gl 1464966 NP_068807 Chr:17q25.2 9.02888 0.095261 sphingosine kinase 175 219257_s_at SPHK1 sphingosine kinase 1 /// NMJ) 21972 gl 1464966 NP_068807 Chr: 17q25.2 9.02888 0.095261 sphingosine kinase 1

76 205403_at IL1R2 interleukin 1 receptor, type Il /// NM_004633 g4758597 NP_004624 Chr:2q12-q22 8.48697 4.174189 interleukin 1 receptor, type Il76 205403_at IL1R2 interleukin 1 receptor, type II /// NM_004633 g4758597 NP_004624 Chr: 2q12-q22 8.48697 4.174189 interleukin 1 receptor, type II

77 208511_at PTTG2 pituitary tυmor-transforming 2 /// NMJ521OOO gl 0337606 NP_006598 Chr:4p12 8.39545 4.149813 pituitary tumor-transforming 277 208511_at PTTG2 pituitary thoracic transformation 2 /// NMJ521OOO gl 0337606 NP_006598 Chr: 4p12 8.39545 4.149813 pituitary tumor-transforming 2

78 229479 at — Transcribed sequences AI739132 Hs.152812.0 — — 8.30385 2.0898978 229479 at - Transcribed sequences AI739132 Hs.152812.0 - - 8.30385 2.08989

79 203454_s_at ATOX1 ATX1 antioxidant protein 1 NM_004045 g4757803 NPJ0O4O36 Chr:5q32 8.13105 2.009521 homolog (yeast) /// ATX1 antioxidant protein 1 homolog (yeast)79 203454_s_at ATOX1 ATX1 antioxidant protein 1 NM_004045 g4757803 NPJ0O4O36 Chr: 5q32 8.13105 2.009521 homologue (yeast) /// ATX1 antioxidant protein 1 homologue (yeast)

80 204709_s_at KIF23 kinesin family member 23 /// NM_004856 gl 3699831 NP_004847 Chr:15q22.31 8.06008 2.814919 kinesin family member 2380 204709_s_at KIF23 kinesin family member 23 /// NM_004856 gl 3699831 NP_004847 Chr: 15q22.31 8.06008 2.814919 kinesin family member 23

81 222155_s_at FLJ 11856 putative G-protein coupled AK021918 Hs.6459.1 NP_078807 Chr:8q24.3 8.01605 9.625715 receptor GPCR4181 222155_s_at FLJ 11856 putative G-protein coupled AK021918 Hs.6459.1 NP_078807 Chr: 8q24.3 8.01605 9.625715 receptor GPCR41

82 225091_at C20orf99 chromosome 20 open reading AL034548 Hs.28608.0 NPJ 49080 Chr:20p13- 7.92124 0.518167 frame 99 p12.282 225091_at C20orf99 chromosome 20 open reading AL034548 Hs.28608.0 NPJ 49080 Chr: 20p13- 7.92124 0.518167 frame 99 p12.2

83 202478_at TRIB2 tribbles homolog 2 (Drosophila) NM_021643 gl 1056053 NP_067675 Chr:2p25.1 7.86235 3.612633 83 202478_at TRIB2 tribbles homolog 2 (Drosophila) NM_021643 gl 1056053 NP_067675 Chr: 2p25.1 7.86235 3.612633

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

84 223381 at CDCA1 cell division cycle associated 1 AF326731 gl 2667400 NP 113611 Chr:1q23.2 7.18643 12.8021984 223381 at CDCA1 cell division cycle associated with 1 AF326731 gl 2667400 NP 113611 Chr: 1q23.2 7.18643 12.80219

85 221521_s_at Pfs2 DNA replication complex GINS BC003186 gl 3112024 NP_057179 Chr:16q24.1 6.96637 1.578435 protein PSF2 /// DNA replication complex GINS protein PSF285 221521_s_at Pfs2 DNA replication complex GINS BC003186 gl 3112024 NP_057179 Chr: 16q24.1 6.96637 1.578435 protein PSF2 /// DNA replication complex GINS protein PSF2

86 200634 at PFN 1 profilin 1 /// profilin 1 NM 005022 g4826897 NP 005013 Chr:17p13.3 6.76391 13.4032986 200634 at PFN 1 profilin 1 /// profilin 1 NM 005022 g4826897 NP 005013 Chr: 17p13.3 6.76391 13.40329

87 223452 s at Hs.27099.0 6.63353 2.48911787 223452 s at Hs.27099.0 6.63353 2.489117

88 205909_at POLE2 Polymerase (DNA directed), NMJD02692 g4505934 NP_002683 Chr:14q21-q22 6.58509 1.578435 epsilon 2 (p59 subunit) /// Polymerase (DNA directed), epsilon 2 (p59 subunit)88 205909_at POLE2 polymerase (DNA directed), NMJD02692 g4505934 NP_002683 Chr: 14q21-q22 6.58509 1.578435 epsilon 2 (p59 subunit) /// polymerase (DNA directed), epsilon 2 (p59 subunit)

89 235147_at — CDNA FLJ43167 fis, clone R56118 Hs.177788.0 — — 6.58167 3.306454 FCBBF300404189 235147_at - CDNA FLJ43167 fis, clone R56118 Hs.177788.0 - - 6.58167 3.306454 FCBBF3004041

90 232578 at Hs.16762.1 6.51591 4.17418990 232578 at Hs.16762.1 6.51591 4.174189

91 216228_s_at WDHD1 WD repeat and HMG-box DNA AK001538 Hs.72160.1 NP_009017 Chr:14q22.2 6.46661 8.779357 binding protein 191 216228_s_at WDHD1 WD repeat and HMG-box DNA AK001538 Hs.72160.1 NP_009017 Chr: 14q22.2 6.46661 8.779357 binding protein 1

92 206023 at NMU neuromedin U /// neuromedin U NM 006681 g5729946 NP 006672 Chr:4q12 5.87566 0.62566392 206023 at NMU neuromedin U /// neuromedin U NM 006681 g5729946 NP 006672 Chr: 4q12 5.87566 0.625663

93 231280 at — Transcribed sequences AW139091 Hs.161158.0 — — 5.81714 2.0898993 231280 at - Transcribed sequences AW139091 Hs.161158.0 - - 5.81714 2.08989

94 229070_at C6orf105 chromosome 6 open reading AA470369 Hs.72115.0 NPJ 16133 Chr:6p24.1 5.8163 1.223189 frame 10594 229070_at C6orf105 chromosomes 6 open reading AA470369 Hs.72115.0 NPJ 16133 Chr: 6p24.1 5.8163 1.223189 frame 105

95 238808_at HOXA13 homeo box A13 R33750 Hs.68826.0 NP 000513 Chr:7p15-p14 5.77611 2.48911795 238808_at HOXA13 homeo box A13 R33750 Hs.68826.0 NP 000513 Chr: 7p15-p14 5.77611 2.489117

96 236044_at PPAPDC1 phosphatidic acid Phosphatase BF130943 Hs.40479.0 — Chr:10q26.13 5.73324 2.814919 type 2 domain containing 196 236044_at PPAPDC1 phosphatidic acid phosphatase BF130943 Hs.40479.0 - Chr: 10q26.13 5.73324 2.814919 type 2 domain containing 1

97 235178 x at AL120674 Hs.265592.0 5.61632 2.48911797 235178 x at AL120674 Hs.265592.0 5.61632 2.489117

98 219364_at LGP2 likely ortholog of mouse NM_024119 g13129141 NP_077024 Chr:17q21.31 5.56982 2.489117 D11lgp298 219364_at LGP2 likely orthologue of mouse NM_024119 g13129141 NP_077024 Chr: 17q21.31 5.56982 2.489117 D11lgp2

99 200755 s at CALU calumenin BF939365 Hs.7753.0 NP 001210 Chr:7q32 5.52713 4.43674499 200755 s at CALU calumenin BF939365 Hs.7753.0 NP 001210 Chr: 7q32 5.52713 4.436744

100 206452_x_at PPP2R4 protein Phosphatase 2A, NM_021131 gl 0880986 NP_066954 Chr:9q34 5.36719 3.62324 regulatory subunit B' (PR 53)100 206452_x_at PPP2R4 protein phosphatase 2A, NM_021131 gl 0880986 NP_066954 Chr: 9q34 5.36719 3.62324 regulatory subunit B '(PR 53)

101 228033 at E2F7 E2F transcription factor 7 AI341146 Hs.144687.0 NP 976328 Chr:12q21.1 5.34918 1.702877101 228033 at E2F7 E2F transcription factor 7 AI341146 Hs.144687.0 NP 976328 Chr: 12q21.1 5.34918 1.702877

102 212398 at Hs.263671.0 5.24146 4.830969102 212398 at Hs.263671.0 5.24146 4.830969

103 204822_at TTK TTK protein kinase /// TTK NM_003318 g4507718 NP_003309 Chr:6q13-q21 5.18259 3.612633 protein kinase103 204822_at TTK TTK protein kinase /// TTK NM_003318 g4507718 NP_003309 Chr: 6q13-q21 5.18259 3.612633 protein kinase

104 239108 at H16791 Hs.31445.0 4.92619 1.901012104 239108 at H16791 Hs.31445.0 4.92619 1.901012

105 219555_s_at BM039 uncharacterized bone marrow NM_018455 g8922096 NP_060925 Chr:16q23.2 4.92008 1.223189 protein BM039 105 219555_s_at BM039 uncharacterized bone marrow NM_018455 g8922096 NP_060925 Chr: 16q23.2 4.92008 1.223189 protein BM039

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

106 210052 s at TPX2 TPX2, microtubule-associated AF098158 g6073830 NP_036244 Chr:20q11.2 4.89931 3.612633 protein homolog (Xenopus laevis) /// TPX2, microtubule- associated protein homolog (Xenopus laevis)106 210052 s at TPX2 TPX2, microtubule-associated AF098158 g6073830 NP_036244 Chr: 20q11.2 4.89931 3.612633 protein homologue (Xenopus laevis) /// TPX2, microtubule-associated protein homologue (Xenopus laevis)

107 202107 s at MCM2 MCM2 minichromosome NM_004526 g4758705 NP_004517 Chr:3q21 4.83182 1.578435 maintenance deficient 2, mitotin (S. cerevisiae) /// MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)107 202107 s at MCM2 MCM2 minichromosomes NM_004526 g4758705 NP_004517 Chr: 3q21 4.83182 1.578435 maintenance deficient 2, mitotine (S. cerevisiae) /// MCM2 minichromosomes maintenance deficient 2, mitotine (S. cerevisiae)

108 209172_s_at CENPF centromere protein F, U30872 gl 000093 NP_057427 Chr:1q32-q41 4.77099 4.436744 350/400ka (mitosin) /// centromere protein F1 350/400ka (mitosin)108 209172_s_at CENPF centromere protein F, U30872 gl 000093 NP_057427 Chr: 1q32-q41 4.77099 4.436744 350 / 400ka (mitosine) /// centromeric protein F 1 350 / 400ka (mitosin)

109 221922_at GPSM2 G-protein signalling modulator 2 AW195581 Hs.93121.1 NP_037428 Chr.ip13.3 4.73028 13.40329 (AGS3-Iike, C. elegans)109 221922_at GPSM2 G-protein signaling modulator 2 AW195581 Hs.93121.1 NP_037428 Chr.ip13.3 4.73028 13.40329 (AGS3-Iike, C. elegans)

110 203256_at CDH3 Cadherin 3, type 1 , P-cadherin NM_001793 g4502722 NP_001784 Chr:16q22.1 4.6955 0.625663 (placental) /// Cadherin 3, type 1 , P-cadherin (placental)110 203256_at CDH3 Cadherin 3, type 1, P-cadherin NM_001793 g4502722 NP_001784 Chr: 16q22.1 4.6955 0.625663 (placental) /// Cadherin 3, type 1, P-cadherin (placental)

111 218960_at TMPRSS4 transmembrane protease, NM_016425 g7705976 NP_063947 Chr:11q23.3 4.64396 4.830969 serine 4 /// transmembrane protease, serine 4111 218960_at TMPRSS4 transmembrane protease, NM_016425 g7705976 NP_063947 Chr: 11q23.3 4.64396 4.830969 serine 4 /// transmembrane protease, serine 4

112 222958 s at DEPDC1 DEP domain containing 1 AK000490 Hs.133260.0 NP 060249 Chr:1p31.2 4.63101 4.640066112 222958 s at DEPDC1 DEP domain containing 1 AK000490 Hs.133260.0 NP 060249 Chr: 1p31.2 4.63101 4.640066

113 204891_s_at LCK iymphocyte-specific protein NM_005356 g4885448 NP_005347 Chr:1p34.3 4.55951 14.22309 tyrosine kinase /// Iymphocyte- specific protein tyrosine kinase113 204891_s_at LCK iymphocyte-specific protein NM_005356 g4885448 NP_005347 Chr: 1p34.3 4.55951 14.22309 tyrosine kinase /// Iymphocyte-specific protein tyrosine kinase

114 205044_at GABRP gamma-aminobutyric acid NM_014211 g7657105 NP_055026 Chr:5q33-q34 4.54226 1.901012 (GABA) A receptor, pi /// gamma-aminobutyric acid (GABA) A receptor, pi114 205044_at GABRP gamma-aminobutyric acid NM_014211 g7657105 NP_055026 Chr: 5q33-q34 4.54226 1.901012 (GABA) A receptor, pi /// gamma-aminobutyric acid (GABA) A receptor, pi

115 208932_at PPP4C protein Phosphatase 4 (formerly BC001416 gl 2655126 NP_002711 Chr:16p12- 4.53699 3.612633 X), catalytic subunit /// protein 16p11 Phosphatase 4 (formerly X), catalytic subunit115 208932_at PPP4C protein phosphatase 4 (formerly BC001416 gl 2655126 NP_002711 Chr: 16p12- 4.53699 3.612633 X), catalytic subunit /// protein 16p11 phosphatase 4 (formerly X), catalytic subunit

116 221122_at HRASLS2 HRAS-like suppressor 2 NM_017878 g8923525 NP_060348 Chr:11q13.1 4.45192 1.380569 116 221122_at HRASLS2 HRAS-like suppressor 2 NM_017878 g8923525 NP_060348 Chr: 11q13.1 4.45192 1.380569

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

117 212992_at C14orf78 chromosome 14 open reading AI935123 Hs.57548.0 — Chr:14q32.33 4.41878 4.149813 frame 78117 212992_at C14orf78 chromosomes 14 open reading AI935123 Hs.57548.0 - Chr: 14q32.33 4.41878 4.149813 frame 78

118 232434__at — CDNA FLJ 14199 fis, clone AA160945 Hs.14479.0 — — 4.4086 1.223189 NT2RP3002713118 232434__at - CDNA FLJ 14199 fis, clone AA160945 Hs.14479.0 - - 4.4086 1.223189 NT2RP3002713

119 243854_at — Transcribed sequences AI094222 Hs.166572.0 — — 4.35379 3.62324119 243854_at - Transcribed sequences AI094222 Hs.166572.0 - - 4.35379 3.62324

120 206560_s_at MlA melanoma inhibitory activity /// NM_006533 g5729924 NP_006524 Chr:19q13.32- 4.34766 3.62324 melanoma inhibitory activity q13.33MAL melanoma inhibitory activity /// NM_006533 g5729924 NP_006524 Chr: 19q13.32- 4.34766 3.62324 melanoma inhibitory activity q13.33

121 214135 at Hs.16762.0 4.30049 1.702877121 214135 at Hs.16762.0 4.30049 1.702877

122 208002_s_at BACH brain acyl-CoA hydrolase /// NM_007274 g6857796 NP_009205 Chr:1p36.31- 4.26931 3.612633 brain acyl-CoA hydrolase p36.11122 208002_s_at BACH brain acyl-CoA hydrolase /// NM_007274 g6857796 NP_009205 Chr: 1p36.31- 4.26931 3.612633 brain acyl-CoA hydrolase p36.11

123 222848_at FKSG14 leucine zipper protein FKSG14 BC005400 gl 3529292 NPJD71428 Chr:5p15.2- 4.25637 0.779487 q12.3123 222848_at FKSG14 leucine zipper protein FKSG14 BC005400 gl 3529292 NPJD71428 Chr: 5p15.2- 4.25637 0.779487 q12.3

124 201890_at RRM2 ribonucleotide reductase M2 BE966236 Hs.75319.0 NPJ)OI 025 Chr:2p25-p24 4.23756 1.578435 Polypeptide124 201890_at RRM2 ribonucleotide reductase M2 BE966236 Hs.75319.0 NPJ) OI 025 Chr: 2p25-p24 4.23756 1.578435 Polypeptides

125 218886_at PAK1 IP1 PAK1 interacting protein 1 NM 017906 g8923576 NP 060376 Chr:6p24.1 4.23644 3.612633125 218886_at PAK1 IP1 PAK1 interacting protein 1 NM 017906 g8923576 NP 060376 Chr: 6p24.1 4.23644 3.612633

126 217777 s at HSPC121 butyrate-induced transcript 1 NM 016395 g7705458 NP 057479 Chr:15q22.2 4.21403 6.991358126 217777 s at HSPC121 butyrate-induced transcript 1 NM 016395 g7705458 NP 057479 Chr: 15q22.2 4.21403 6.991358

127 204653_at TFAP2A transcription factor AP-2 alpha BF343007 Hs.18387.0 NP_003211 Chr:6p24 4.20146 4.640066 (activating enhancer binding protein 2 alpha)127 204653_at TFAP2A transcription factor AP-2 alpha BF343007 Hs.18387.0 NP_003211 Chr: 6p24 4.20146 4.640066 (activating enhancer binding protein 2 alpha)

128 218925_s_at C11orf1 chromosome 11 open reading NM_022761 gl 2232430 NP_073598 Chr:11q13-q22 4.04932 7.646445 frame 1128 218925_s_at C11orf1 chromosomes 11 open reading NM_022761 gl 2232430 NP_073598 Chr: 11q13-q22 4.04932 7.646445 frame 1

129 203764_at DLG7 discs, large homolog 7 NM_014750 g7661851 NP_055565 Chr:14q22.2 4.00966 2.571322 (Drosophila)129 203764_at DLG7 discs, large homologue 7 NM_014750 g7661851 NP_055565 Chr: 14q22.2 4.00966 2.571322 (Drosophila)

130 206482_at PTK6 PTK6 protein tyrosine kinase 6 NM_005975 g5174646 NP_005966 Chr:20q13.3 3.99446 2.08989 /// PTK6 protein tyrosine kinase 6130 206482_at PTK6 PTK6 protein tyrosine kinase 6 NM_005975 g5174646 NP_005966 Chr: 20q13.3 3.99446 2.08989 /// PTK6 protein tyrosine kinase 6

131 216397 s at BOP1 block of proliferation 1 AK024840 Hs.30736.1 NP 056016 Chr:8q24.3 3.95948 13.40329131 216397 s at BOP1 block of proliferation 1 AK024840 Hs30736.1 NP 056016 Chr: 8q24.3 3.95948 13.40329

132 23071 O_at — CDNA FLJ41489 fis, clone W05495 Hs.218710.0 — — 3.9591 0.518167 BRTHA2004582132 23071 O_at - CDNA FLJ41489 fis, clone W05495 Hs.218710.0 - - 3.9591 0.518167 BRTHA2004582

133 218308_at TACC3 transforming, acidic coiled-coil NM_006342 g5454101 NP_006333 Chr:4p16.3 3.94462 1.702877 containing protein 3 /// transforming, acidic coiled-coil containing protein 3133 218308_at TACC3 transforming, acidic coiled-coil NM_006342 g5454101 NP_006333 Chr: 4p16.3 3.94462 1.702877 containing protein 3 /// transforming, acidic coiled-coil containing protein 3

134 234412_at Hs.274565.0 3.93128 1.702877134 234412_at Hs.274565.0 3.93128 1.702877

135 238022_at — LOC388279 (LOC388279), AA954994 Hs.237396.1 — Chr:16q12.2 3.91553 2.08989 mRNA 135 238022_at - LOC388279 (LOC388279), AA954994 Hs.237396.1 - Chr: 16q12.2 3.91553 2.08989 mRNA

Nr. Affymetrϊx Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrϊx Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

136 219836_at ZBED2 zinc finger, BED domain NM_024508 gl 3375643 NP_078784 Chr:3q13.13 3.89015 13.40329 containing 2136 219836_at ZBED2 zinc finger, BED domain NM_024508 gl 3375643 NP_078784 Chr: 3q13.13 3.89015 13.40329 containing 2

137 204507_s_at PPP3R1 protein Phosphatase 3 (formerly NM_000945 g4506024 NP_000936 Chr:2p15 3.86121 3.612633 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I)137 204507_s_at PPP3R1 protein phosphatase 3 (formerly NM_000945 g4506024 NP_000936 Chr: 2p15 3.86121 3.612633 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I)

138 218585_s_at RAMP RA-regulated nuclear matrix- NM_016448 g7705575 NP_057532 Chr:1q32.1- 3.78703 2.571322 associated protein q32.2138 218585_s_at RAMP RA-regulated nuclear matrix NM_016448 g7705575 NP_057532 Chr: 1q32.1- 3.78703 2.571322 associated protein q32.2

139 205449_at HSU79266 protein predicted by cione NMJD13299 g9558738 NPJ337431 Chr:11q13.1 3.78561 8.779357 23627139 205449_at HSU79266 protein predicted by cione NMJD13299 g9558738 NPJ337431 Chr: 11q13.1 3.78561 8.779357 23627

140 201564 s at FSCN1 fascin homolog 1 , actin- NM 003088 g4507114 NP__003079 Chr:7p22 3.78146 2.08989 bundling protein (Strongylocentrotus purpuratus) /// fascin homolog 1 , actin- bundling protein (Strongylocentrotus purpuratus)140 201564 s at FSCN1 fascin homologue 1, actin-NM 003088 g4507114 NP__003079 Chr: 7p22 3.78146 2.08989 bundling protein (Strongylocentrotus purpuratus) /// fascin homolog 1, actin bundling protein (Strongylocentrotus purpuratus)

141 218795_at ACP6 lysophosphatidic acid NM_016361 g7705954 NP. _057445 Chr:1q21 3.76905 0.149911 Phosphatase /// W lysophosphatidic acid Phosphatase141 218795_at ACP6 lysophosphatidic acid NM_016361 g7705954 NP . _057445 Chr: 1q21 3.76905 0.149911 Phosphatase /// W lysophosphatidic acid phosphatase

142 209132 s at COMMD4 COMM domain containing 4 BE313890 Hs.18490.1 NP 060298 Chr:15q23 3.76573 0.353669142 209132 s at COMMD4 COMM domain containing 4 BE313890 Hs.18490.1 NP 060298 Chr: 15q23 3.76573 0.353669

143 213906_at MYBL1 v-myb myeloblastosis viral AW592266 Hs.300592.0 Chr:8q22 3.75595 12.53374 oncogene homolog (avian)-like 1143 213906_at MYBL1 v-myb myeloblastosis viral AW592266 Hs.300592.0 Chr: 8q22 3.75595 12.53374 oncogene homologue (avian) -like 1

144 224753 at CDCA5 cell division cycle associated 5 BE614410 Hs.23044.2 NP 542399 Chr:11q12.1 3.7321 1.223189144 224753 at CDCA5 cell division cycle associated 5 BE614410 Hs.23044.2 NP 542399 Chr: 11q12.1 3.7321 1.223189

145 208209_s_at C4BPB complement component 4 NM_000716 g4502504 NP. _000707 Chr:1q32 3.72496 3.62324 binding protein, beta /// complement component 4 binding protein, beta145 208209_s_at C4BPB complement component 4 NM_000716 g4502504 NP . _000707 Chr: 1q32 3.72496 3.62324 binding protein, beta /// complement component 4 binding protein, beta

146 219148 at g8923876 3.67803 2.571322146 219148 at g8923876 3.67803 2.571322

147 225362_at LOC159090 similar to hypothetical protein AI341165 Hs.288550.0 NP. _660327 Chr:xq26.3 3.66805 13.5285 MGC17347Similar to hypothetical protein AI341165 Hs.288550.0 NP . _660327 Chr: xq26.3 3.66805 13.5285 MGC17347

148 202589_at TYMS thymidylate synthetase /// NM_001071 g4507750 NP. _001062 Chr:18p11.32 3.6482 1.578435 thymidylate synthetase 148 202589_at TYMS thymidylate synthetase /// NM_001071 g4507750 NP . _001062 Chr: 18p11.32 3.6482 1.578435 thymidylate synthetase

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

149 209715_at CBX5 chromobox homolog 5 (HP1 L07515 gl 84310 NP_036249 Chr:12q13.13 3.64483 8.153992 alpha homolog, Drosophila) /// chromobox homolog 5 (HP1 alpha homolog, Drosophila)149 209715_at CBX5 chromobox homologue 5 (HP1 L07515 gl 84310 NP_036249 Chr: 12q13.13 3.64483 8.153992 alpha homolog, Drosophila) /// chromobox homolog 5 (HP1 alpha homolog, Drosophila)

150 211430_s_at IGHG1 Immunoglobulin heavy constant M87789 gl 85361 — Chr:14q32.33 3.64005 12.53374 gamma 1 (G 1m marker)150 211430_s_at IGHG1 immunoglobulin heavy constant M87789 gl 85361 - Chr: 14q32.33 3.64005 12.53374 gamma 1 (G 1m marker)

151 238017_at RDH-E2 retinal Short chain AI440266 Hs.170673.0 NP_620419 Chr:8q12.1 3.63987 9.266777 dehydrogenase reductase151 238017_at RDH-E2 retinal short chain AI440266 Hs.170673.0 NP_620419 Chr: 8q12.1 3.63987 9.266777 dehydrogenase reductase

152 209405_s_at FAM3A family with sequence similarity BC002934 gl 2804156 NP_068578 Chr:xq28 3.60742 4.149813 3, member A152 209405_s_at FAM3A family with sequence similarity BC002934 gl 2804156 NP_068578 Chr: xq28 3.60742 4.149813 3, member A

153 226545_at Hs.94030.0 3.58626 4.174189153 226545_at Hs.94030.0 3.58626 4.174189

154 243106_at MICL myeloid inhibitory C-type lectin- AA916861 Hs.190519.0 NP_612210 Chr:12p13.31 3.57836 2.08989 like receptor154 243106_at MICL myeloid inhibitory C-type lectin AA916861 Hs.190519.0 NP_612210 Chr: 12p13.31 3.57836 2.08989 like receptor

155 229555_at GALNT5 UDP-N-acetyl-alpha-D- AI633503 Hs.55968.0 NP_055383 Chr:2q24.2 3.57656 2.08989 galactosamine:polypeptide N- acetylgalactosaminyltransferase 5 (GalNAc-T5)155 229555_at GALNT5 UDP-N-acetyl-alpha-D-Al633503 Hs.55968.0 NP_055383 Chr: 2q24.2 3.57656 2.08989 galactosamine: polypeptide N-acetylgalactosaminyltransferase 5 (GalNAc-T5)

156 227342_s_at AA621983 Hs.116051.0 3.56448 1.901012156 227342_s_at AA621983 Hs.116051.0 3.56448 1.901012

157 230795_at HIST2H4 histone 2, H4 AI828075 Hs.55468.0 NP 003539 Chr:1q21 3.55684 1.702877157 230795_at HIST2H4 histone 2, H4 AI828075 Hs.55468.0 NP 003539 Chr: 1q21 3.55684 1.702877

158 206858_s_at HOXC6 homeo box C6 /// homeo box NM_004503 g4758553 NP_004494 Chr:12q13.3 3.55228 3.612633 C6158 206858_s_at HOXC6 homeo box C6 /// homeo box NM_004503 g4758553 NP_004494 Chr: 12q13.3 3.55228 3.612633 C6

159 205372_at PLAG1 pleiomorphic adenoma gene 1 NM_002655 g4505854 NP_002646 Chr:8q12 3.55106 10.20301 /// pleiomorphic adenoma gene 1159 205372_at PLAG1 pleiomorphic adenoma gene 1 NM_002655 g4505854 NP_002646 Chr: 8q12 3.55106 10.20301 /// pleiomorphic adenoma gene 1

160 222740_at ATAD2 ATPase family, AAA domain AI925583 Hs.46677.0 NP_054828 Chr:8q24.13 3.53755 13.5285 containing 2160 222740_at ATAD2 ATPase family, AAA domain AI925583 Hs.46677.0 NP_054828 Chr: 8q24.13 3.53755 13.5285 containing 2

161 205180_s_at ADAM8 a disintegrin and NM_001109 g4557252 NP_001100 Chr:10q26.3 3.50386 1.702877 metalloproteinase domain 8 /// a disintegrin and metalloproteinase domain 8161 205180_s_at ADAM8 a disintegrin and NM_001109 g4557252 NP_001100 Chr: 10q26.3 3.50386 1.702877 metalloproteinase domain 8 /// a disintegrin and metalloproteinase domain 8

162 201933_at PCOLN3 procollagen (type III) N- NM_002768 g4506138 NP_002759 Chr:16q24.3 3.49136 7.646445 endopeptidase /// procollagen (type III) N-endopeptidase162 201933_at PCOLN3 procollagen (type III) N- NM_002768 g4506138 NP_002759 Chr: 16q24.3 3.49136 7.646445 endopeptidase /// procollagen (type III) N-endopeptidase

163 212147_at EST1B Est1p-Iike protein B AB029012 Hs.4990.0 NP 056142 Chr:1q21.2 3.48783 3.62324163 212147_at EST1B Est1p-Iike protein B AB029012 Hs.4990.0 NP 056142 Chr: 1q21.2 3.48783 3.62324

164 220060_s_at FLJ20641 hypothetical protein FLJ20641 NM_017915 g8923595 NP 060385 Chr:12q23.3 3.47902 1.578435164 220060_s_at FLJ20641 hypothetical protein FLJ20641 NM_017915 g8923595 NP 060385 Chr: 12q23.3 3.47902 1.578435

165 243409_at — Transcribed sequences AI005407 Hs.130645.0 — — 3.47284 13.64212 165 243409_at - Transcribed sequences AI005407 Hs.130645.0 - - 3.47284 13.64212

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

166 202784_s_at NNT nicotinamide nucieotide NM_012343 g6912535 NP_036475 Chr:5p13.1- 3.43463 1.578435 transhydrogenase 5cen166 202784_s_at NNT nicotinamide nucieotide NM_012343 g6912535 NP_036475 Chr: 5p13.1- 3.43463 1.578435 transhydrogenase 5cene

167 242881_x_at BG285837 Hs.317584.1 3.43265 4.640066167 242881_x_at BG285837 Hs.317584.1 3.43265 4.640066

168 221690_s_at NALP2 NACHT, leucine rieh repeat and AF298547 gl 0198206 NP_060322 Chr:19q13.42 3.38239 11.06357 PYD containing 2168 221690_s_at NALP2 NIGHT, leucine repeat repeat and AF298547 gl 0198206 NP_060322 Chr: 19q13.42 3.38239 11.06357 PYD containing 2

169 241726_at HLCS holocarboxylase synthetase AI682088 Hs.223368.0 NP_000402 Chr:21q22.1 3.36768 2.814919 (biotin-[proprionyl-Coenzyme A- carboxylase (ATP-hydrolysing)] ligase)169 241726_at HLCS holocarboxylase synthetase AI682088 Hs.223368.0 NP_000402 Chr: 21q22.1 3.36768 2.814919 (biotin [proprionyl-coenzyme A carboxylase (ATP-hydrolysing)] ligase)

170 205167_s_at CDC25C cell division eyele 25C /// cell NMJ)01790 gl 2408659 NP_001781 Chr:5q31 3.36325 4.149813 division eyele 25C170 205167_s_at CDC25C cell division eyele 25C /// cell NMJ) 01790 gl 2408659 NP_001781 Chr: 5q31 3.36325 4.149813 division eyele 25C

171 231964_at Hs.137206.0 3.35534 9.266777171 231964_at Hs.137206.0 3.35534 9.266777

172 204026_s_at ZWlNT ZW10 interactor /// ZW10 NM_007057 g6857828 NP_008988 Chr:10q21-q22 3.34393 1.578435 interactor172 204026_s_at ZWlNT ZW10 interactor /// ZW10 NM_007057 g6857828 NP_008988 Chr: 10q21-q22 3.34393 1.578435 interactor

173 206364_at KIF14 kinesin family member 14 NM 014875 g7661877 — Chr:1pter-q31.3 3.34244 6.991358173 206364_at KIF14 kinesin family member 14 NM 014875 g7661877 - Chr: 1pter-q31.3 3.34244 6.991358

174 237690 at GPR115 G protein-coupled receptor 115 W67511 Hs.57887.0 NP_722580 Chr:6p12.3 3.33512 2.08989174 237690 at GPR115 G protein-coupled receptor 115 W67511 Hs.57887.0 NP_722580 Chr: 6p12.3 3.33512 2.08989

175 222847_s_at EGLN3 egl niπe homolog 3 (C. elegans) AI378406 Hs.18878.0 NP_071356 Chr:14q13.1 3.3307 1.051298175 222847_s_at EGLN3 egl niπe homologue 3 (C. elegans) AI378406 Hs.18878.0 NP_071356 Chr: 14q13.1 3.3307 1.051298

176 203234_at UPP1 uridine Phosphorylase 1 /// NM_003364 g4507838 NP_003355 Chr:7p12.3 3.3101 3.61384 °" uridine Phosphorylase 1176 203234_at UPP1 uridine Phosphorylase 1 /// NM_003364 g4507838 NP_003355 Chr: 7p12.3 3.3101 3.61384 ° "uridine Phosphorylase 1

177 223278_at GJB2 gap junction protein, beta 2, M86849 g4481752 NP_003995 Chr:13q11-q12 3.29647 0.625663 26kDa (connexin 26)177 223278_at GJB2 gap junction protein, beta 2, M86849 g4481752 NP_003995 Chr: 13q11-q12 3.29647 0.625663 26kDa (connexin 26)

178 204875_s_at GMDS GDP-mannose 4,6-dehydratase NM_001500 g4504030 NP_001491 Chr:6p25 3.29477 7.646445 /// GDP-mannose 4,6- dehydratase178 204875_s_at GMDS GDP-mannose 4,6-dehydratase NM_001500 g4504030 NP_001491 Chr: 6p25 3.29477 7.646445 /// GDP-mannose 4,6-dehydratase

179 239579_at ABHD7 abhydrolase domain containing AI807532 Hs.201555.0 NP_775838 Chr:1p22.1 3.29122 4.640066 7179 239579_at ABHD7 abhydrolase domain containing AI807532 Hs.201555.0 NP_775838 Chr: 1p22.1 3.29122 4.640066 7

180 40472_at LOC254531 PISC domain containing AF007155 4884817_RC NP_705841 Chr:15q13.2 3.29069 3.306454 hypothetical protein180 40472_at LOC254531 PISC domain containing AF007155 4884817_RC NP_705841 Chr: 15q13.2 3.29069 3.306454 hypothetical protein

181 207556_s_at DGKZ diaeylglycerol kinase, zeta NM_003646 g4503316 NP_003637 Chr:11p11.2 3.28637 4.174189 104kDa181 207556_s_at DGKZ diaeylglycerol kinase, zeta NM_003646 g4503316 NP_003637 Chr: 11p11.2 3.28637 4.174189 104kDa

182 229927 at LOC93273 hypothetical protein LOC93273 BE222220 Hs.104830.0 — Chr:1q32.1 3.28619 1.380569LOC93273 BE222220 Hs.104830.0 - Chr: 1q32.1 3.28619 1.380569

183 202311_s_at COL1A1 collagen, type I, alpha 1 AI743621 Hs.172928.0 NP_000079 Chr:17q21.3- 3.28338 14.22309 q22.1183 202311_s_at COL1A1 collagen, type I, alpha 1 AI743621 Hs.172928.0 NP_000079 Chr: 17q21.3- 3.28338 14.22309 q22.1

184 202998_s_at LOXL2 lysyl oxidase-like 2 /// lysyl NM_002318 g4505010 NP_002309 Chr.8p21.3- 3.28288 2.571322 oxidase-like 2 P21.2 184 202998_s_at LOXL2 lysyl oxidase-like 2 /// lysyl NM_002318 g4505010 NP_002309 Chr.8p21.3- 3.28288 2.571322 oxidase-like 2 P21.2

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

185 204962_s_at CENPA centromere protein A, 17kDa /// NM_001809 g4585861 NP_001800 Chr:2p24-p21 3.25273 2.571322 centromere protein A, 17kDa185 204962_s_at CENPA centromere protein A, 17kDa /// NM_001809 g4585861 NP_001800 Chr: 2p24-p21 3.25273 2.571322 centromeric protein A, 17kDa

186 202668_at EFNB2 ephrin-B2 BF001670 Hs.30942.0 NP_004084 Chr:13q33 3.24909 1.578435186 202668_at EFNB2 ephrin B2 BF001670 Hs.30942.0 NP_004084 Chr: 13q33 3.24909 1.578435

187 232164_s_at Hs.200412.0 3.22572 12.80219187 232164_s_at Hs.200412.0 3.22572 12.80219

188 212921_at SMYD2 SET and MYND domain AF070592 Hs.66170.1 NP_064582 Chr:1q32.3 3.21488 13.40329 containing 2188 212921_at SMYD2 SET and MYND domain AF070592 Hs.66170.1 NP_064582 Chr: 1q32.3 3.21488 13.40329 containing 2

189 219918_s_at ASPM asp (abnormal spindle)-like, NM_018123 g8922484 NP_060593 Chr:1q31 3.21184 11.70204 microcephaly associated (Drosophila)189 219918_s_at ASPM asp (abnormal spindle) -like, NM_018123 g8922484 NP_060593 Chr: 1q31 3.21184 11.70204 microcephaly associated (Drosophila)

190 218280_x_at HIST2H2AA histone 2, H2aa NM_003516 g4504250 NP_003507 Chr:1q21.3 3.21072 6.991358190 218280_x_at HIST2H2AA histone 2, H2aa NM_003516 g4504250 NP_003507 Chr: 1q21.3 3.21072 6.991358

191 214455_at HIST1H2BC histone 1 , H2bc /// histone 1 , NM_003526 Hs.239884.0 NP_003517 Chr:6p21.3 3.19049 13.40329 H2bc191 214455_at HIST1H2BC histone 1, H2bc /// histone 1, NM_003526 Hs.239884.0 NP_003517 Chr: 6p21.3 3.19049 13.40329 H2bc

192 204401 at KCNN4 potassium intermediate/small NM_002250 g4504858 NP_002241 Chr:19q13.2 3.16939 4.174189 conductance calcium-activated Channel, subfamily N, member 4 /// potassium intermediate/small conductance calcium-activated Channel, subfamily N, member 4192 204401 at KCNN4 potassium intermediate / small NM_002250 g4504858 NP_002241 Chr: 19q13.2 3.16939 4.174189 conductance calcium-activated channel, subfamily N, member 4 /// potassium intermediate / small conductance calcium-activated channel, subfamily N, member 4

193 226789_at CDNA FLJ46881 fis, clone W84421 Hs.83992.0 Chr:1q21.1 3.16803 0.353669 UTERU3015647, moderately similar to Embigin precursor193 226789_at CDNA FLJ46881 fis, clone W84421 Hs.83992.0 Chr: 1q21.1 3.16803 0.353669 UTERU3015647, moderately similar to Embigin precursor

194 218451_at CDCP1 CUB domain-containing protein NM_022842 gl 2383093 NP. _073753 Chr:3p21.32 3.16611 6.225758 1194 218451_at CDCP1 CUB domain-containing protein NM_022842 gl 2383093 NP . _073753 Chr: 3p21.32 3.16611 6.225758 1

195 228307 at Hs.25897.0 3.1578 1.051298195 228307 at Hs.25897.0 3.1578 1.051298

196 203145_at SPAG5 sperm associated antigen 5 /// NM_006461 g5453631 NP_ _006452 Chr:17q11.2 3.15023 5.685182 sperm associated antigen 5196 203145_at SPAG5 sperm associated antigen 5 /// NM_006461 g5453631 NP_ _006452 Chr: 17q11.2 3.15023 5.685182 sperm associated antigen 5

197 232165 at Hs.200412.0 3.1376 2.814919197 232165 at Hs.200412.0 3.1376 2.814919

198 238632_at — Füll length insert cDNA clone AI631657 Hs.103159.0 — — 3.11543 4.174189 ZD51F08198 238632_at - Fill length insert cDNA clone AI631657 Hs.103159.0 - - 3.11543 4.174189 ZD51F08

199 227449_at EPHA4 EphA4 AI799018 Hs.314784.0 NP 004429 Chr:2q36.1 3.1037 2.489117199 227449_at EPHA4 EphA4 AI799018 Hs.314784.0 NP 004429 Chr: 2q36.1 3.1037 2.489117

200 240943 at — Transcribed sequences AA678195 Hs.180037.0 — — 3.09992 3.62324200 240943 at - Transcribed sequences AA678195 Hs.180037.0 - - 3.09992 3.62324

201 218755_at KIF20A kinesin family member 2OA /// NM_005733 g5032012 NP_ _005724 Chr:5q31 3.07849 4.640066 kinesin family member 2OA 201 218755_at KIF20A kinesin family member 2OA /// NM_005733 g5032012 NP_ _005724 Chr: 5q31 3.07849 4.640066 kinesin family member 2OA

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer JD Protein ID Lokalisierung Change (%)Sample Number JD Protein ID Localization Change (%)

202 219061 s at DXS9879E DNA segment on chromosome NM_006014 g5174432 NP_006005 Chr:xq28 3.07829 6.991358 X (unique) 9879 expressed sequence /// DNA segment on chromosome X (unique) 9879 expressed sequence202 219061 s at DXS9879E DNA segment on chromosomes NM_006014 g5174432 NP_006005 Chr: xq28 3.07829 6.991358 X (unique) 9879 expressed sequence /// DNA segment on chromosomes X (unique) 9879 expressed sequence

203 202705 at CCNB2 cyclin B2 /// cyclin B2 NM 004701 gl 0938017 NP 004692 Chr:15q21.3 3.07247 3.612633203 202705 at CCNB2 cyclin B2 /// cyclin B2 NM 004701 gl 0938017 NP 004692 Chr: 15q21.3 3.07247 3.612633

204 229097. .at Similar to diaphanous homolog AI813331 Hs.283127.0 3.05738 1.702877 3 (Drosophila), clone IMAGE:5277415, mRNA204 229097. .at similar to diaphanous homologue AI813331 Hs.283127.0 3.05738 1.702877 3 (Drosophila), clone IMAGE: 5277415, mRNA

205 223307 at CDCA3 cell division cycle associated 3 BC002551 gl 2803452 NP 112589 Chr:12p13 3.04801 11.55252205 223307 at CDCA3 cell division cycle associated 3 BC002551 gl 2803452 NP 112589 Chr: 12p13 3.04801 11.55252

206 231167. at CDW92 CDW92 antigen AW444881 Hs.77829.0 NP 071392 Chr:9q31.2 3.04541 1.223189206 231167 . at CDW92 CDW92 antigen AW444881 Hs.77829.0 NP 071392 Chr: 9q31.2 3.04541 1.223189

207 205072 s at XRCC4 X-ray repair complementing NM_022406 gl 2408646 NPJ303392 Chr:5q13-q14 3.03445 2.814919 defective repair in Chinese hamster cells 4 /// X-ray repair complementing defective repair in Chinese hamster cells 42072050 s at XRCC4 X-ray repair complementing NM_022406 gl 2408646 NPJ303392 Chr: 5q13-q14 3.03445 2.814919 defective repair in Chinese hamster cells 4 /// X-ray repair complementing defective repair in Chinese hamster cells 4

208 222036 s at MCM4 MCM4 minichromosome AI859865 Hs.154443.1 NPJM35905 Chr:8q11.2 3.02816 3.62324 maintenance deficient 4 (S. cerevisiae)208 222036 s at MCM4 MCM4 minichromosomes AI859865 Hs.154443.1 NPJM35905 Chr: 8q11.2 3.02816 3.62324 maintenance deficient 4 (S. cerevisiae)

209 200085 s at TCEB2 transcription elongation factor B NM_007108 Hs.172772.2 NP_009039 Chr:16p12.3 3.01714 2.009521 (SlII), Polypeptide 2 (18kDa, elongin B) /// transcription elongation factor B (SIII), Polypeptide 2 (18kDa, elongin B) /// transcription elongation factor B (SIII), Polypeptide 2 (18kDa, elongin B)Transcriptional elongation factor B (SII), polypeptides 2 (18kDa, elongin B) /// transcription elongation factor B (SIII), polypeptides 2 (18 kDa, elongin B)

210 204825. _at MELK maternal embryonic leucine NM_014791 g7661973 NP. .055606 Chr:9p13.1 3.00383 4.436744 zipper kinase210 204825. _at MELK maternal embryonic leucine NM_014791 g7661973 NP. .055606 Chr: 9p13.1 3.00383 4.436744 zipper kinase

211 235609. _at BRIP1 BRCA1 interacting protein C- BF056791 Hs.87507.0 NP. .114432 Chr:17q22-q24 2.99293 1.702877 terminal helicase 1211 235609. _at BRIP1 BRCA1 interacting protein C-BF056791 Hs.87507.0 NP . .114432 Chr: 17q22-q24 2.99293 1.702877 terminal helicase 1

212 203755 at BUB1B BUB1 budding uninhibited by NM 001211 g5729749 NP_001202 Chr:15q15 2.97627 4.830969 benzimidazoles 1 homolog beta (yeast) /// BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast) 21219575 at BUB1B BUB1 budding uninhibited by NM 001211 g5729749 NP_001202 Chr: 15q15 2.97627 4.830969 benzimidazoles 1 homologue beta (yeast) /// BUB1 budding uninhibited by benzimidazoles 1 homologue beta (yeast)

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

213 223556 at HELLS helicase, lymphoid-specific AF155827 g8980659 NP 060533 Chr:10q24.2 2.96642 3.306454213 223556 at HELLS helicase, lymphoid-specific AF155827 g8980659 NP 060533 Chr: 10q24.2 2.96642 3.306454

214 219493_at SHCBP1 likely ortholog of mouse Shc NM_024745 gl 3376069 NP_079021 Chr:1βq11.2 2.94762 9.625715 SH2-domain binding protein 1214 219493_at SHCBP1 likely orthologous to mouse Shc NM_024745 gl 3376069 NP_079021 Chr: 1 β q11.2 2.94762 9.625715 SH2-domain binding protein 1

215 202503 s at KIAA0101 KIAA0101 gene product NM 014736 g7661905 NP 055551 Chr:15q22.1 2.94751 1.578435215 202503 s at KIAA0101 KIAA0101 product NM 014736 g7661905 NP 055551 Chr: 15q22.1 2.94751 1.578435

216 205730_s_at ABLIM3 actin binding LIM protein family, NM_014945 g7662329 NP_055760 Chr:5q33.1 2.94559 2.814919 member 3216 205730_s_at ABLIM3 actin binding LIM protein family, NM_014945 g7662329 NP_055760 Chr: 5q33.1 2.94559 2.814919 member 3

217 235670 at — Transcribed sequences AI916948 Hs.28788.0 — — 2.91213 0.779487217 235670 at - Transcribed sequences AI916948 Hs.28788.0 - - 2.91213 0.779487

218 218009_s_at PRC1 protein regulator of cytokinesis NM_003981 g4506038 NP_003972 Chr:15q26.1 2.89253 5.685182 1 /// protein regulator of cytokinesis 1218009_s_at PRC1 protein regulator of cytokinesis NM_003981 g4506038 NP_003972 Chr: 15q26.1 2.89253 5.685182 1 /// protein regulator of cytokinesis 1

219 218883_s_at KLIP1 KSHV latent nuclear antigen NM_024629 gl 3375855 NP_078905 Chr:4q35.1 2.8918 6.991358 interacting protein 1219 218883_s_at KLIP1 KSHV latent nuclear antigen NM_024629 gl 3375855 NP_078905 Chr: 4q35.1 2.8918 6.991358 interacting protein 1

220 208613_s_at FLNB filamin B, beta (actin binding AV712733 Hs.81008.0 NP_001448 Chr:3p14.3 2.88915 14.22309 protein 278)220 208613_s_at FLNB filamin B, beta (actin binding AV712733 Hs.81008.0 NP_001448 Chr: 3p14.3 2.88915 14.22309 protein 278)

221 232200_at HSPA9B heat shock 7OkDa protein 9B AK023317 Hs.254467.0 NP_004125 Chr:5q31.1 2.88381 2.814919 (mortalin-2)221 232200_at HSPA9B heat shock 7OkDa protein 9B AK023317 Hs.254467.0 NP_004125 Chr: 5q31.1 2.88381 2.814919 (mortalin-2)

222 204675 at SRD5A1 steroid-5-alpha-reductase, NM_001047 g4507200 NP_001038 Chr:5p15 2.87979 6.991358 alpha Polypeptide 1 (3-oxo-5 ∞ alpha-steroid delta A- dehydrogenase alpha 1 ) /// steroid-5-alpha-reductase, alpha Polypeptide 1 (3-oxo-5 alpha-steroid delta 4- dehydrogenase alpha 1 )222 204675 at SRD5A1 steroid 5-alpha-reductase, NM_001047 g4507200 NP_001038 Chr: 5p15 2.87979 6.991358 alpha polypeptides 1 (3-oxo-5 ∞ alpha-steroid delta A dehydrogenase alpha 1) /// steroid 5-alpha reductase alpha polypeptides 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)

223 223514, _at CARD11 caspase recruitment domain AF322641 gl 2382772 NP. _115791 Chr:7p22 2.87743 0.625663 family, member 11223 223514 , _at CARD11 caspase recruitment domain AF322641 gl 2382772 NP . _115791 Chr: 7p22 2.87743 0.625663 family, member 11

224 213523 at CCNE1 cyclin E1 AI671049 Hs.9700.0 NP 001229 Chr:19q12 2.87434 6.225758224 213523 at CCNE1 cyclin E1 AI671049 Hs.9700.0 NP 001229 Chr: 19q12 2.87434 6.225758

225 201014_ _s_at PAICS phosphoribosylaminoimidazole NM_006452 g5453538 NP. _006443 Chr:4pter-q21 2.86854 3.612633 carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase225 201014_ _s_at PAICS phosphoribosylaminoimidazole NM_006452 g5453538 NP. _006443 Chr: 4pter-q21 2.86854 3.612633 carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase

226 226777_ _at ADAM12 a disintegrin and AA147933 Hs.8895.0 NP. _003465 Chr:10q26.3 2.84381 4.174189 metalloproteinase domain 12 (meltrin alpha)226 226777_ _at ADAM12 a disintegrin and AA147933 Hs.8895.0 NP. _003465 Chr: 10q26.3 2.84381 4.174189 metalloproteinase domain 12 (meltrin alpha)

227 211926. _s_at MYH9 myosin, heavy Polypeptide 9, AI827941 Hs.146550.0 NP. „002464 Chr:22q13.1 2.80242 11.70204 non-muscle 227 211926. _s_at MYH9 myosin, heavy polypeptides 9, AI827941 Hs.146550.0 NP . " 002464 Chr: 22q13.1 2.80242 11.70204 non-muscle

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

228 210115_at RPL39L ribosomal protein L39-Iike L05096 g388035 NP 443201 Chr:3q27 2.80058 2.489117228 210115_at RPL39L ribosomal protein L39-Iike L05096 g388035 NP 443201 Chr: 3q27 2.80058 2.489117

229 203358_s_at EZH2 enhancer of zeste homolog 2 NM_004456 g4758323 NP_004447 Chr:7q35-q36 2.80041 2.08989 (Drosophila) /// enhancer of zeste homolog 2 (Drosophila)229 203358_s_at EZH2 enhancer of zeste homologue 2 NM_004456 g4758323 NP_004447 Chr: 7q35-q36 2.80041 2.08989 (Drosophila) /// enhancer of zeste homolog 2 (Drosophila)

230 229538_s_at IQGAP3 IQ motif containing GTPase AW271106 Hs.133294.1 NP_839943 Chr:1q23.1 2.77931 0.149911 activating protein 3230 229538_s_at IQGAP3 IQ containing GTPase AW271106 Hs.133294.1 NP_839943 Chr: 1q23.1 2.77931 0.149911 activating protein 3

231 217004_s_at MCF2 MCF.2 cell line derived X13230 Hs.89543.3 NP_005360 Chr:xq27 2.77539 1.578435 transforming sequence /// MCF.2 cell line derived transforming sequence231 217004_s_at MCF2 MCF.2 cell line derived X13230 Hs.89543.3 NP_005360 Chr: xq27 2.77539 1.578435 transforming sequence /// MCF.2 cell line derived transforming sequence

232 214875_x_at APLP2 amyloid beta (A4) precursor-like AW001847 Hs.279518.4 NP_001633 Chr:11q24 2.77101 8.779357 protein 2232 214875_x_at APLP2 amyloid beta (A4) precursor-like AW001847 Hs.279518.4 NP_001633 Chr: 11q24 2.77101 8.779357 protein 2

233 209714 s at CDKW3 cyclin-dependent kinase AF213033 g12734643 NP_005183 Chr:14q22 2.74067 7.646445 inhibitor 3 (CDK2-associated dual specificity Phosphatase) /// cyclin-dependent kinase inhibitor 3 (CDK2-associated dual specificity Phosphatase)233 209714 s at CDKW3 cyclin-dependent kinase AF213033 g12734643 NP_005183 Chr: 14q22 2.74067 7.646445 inhibitor 3 (CDK2-associated dual specificity phosphatase) /// cyclin-dependent kinase inhibitor 3 (CDK2-associated dual specificity phosphatase)

234 205174 s at QPCT glutaminyl-peptide NM_012413 g9257235 NP_036545 Chr:2p22.3 2.74008 4.436744 cyclotransferase (glutaminyl cyclase) /// glutaminyl-peptide cyclotransferase (glutaminyl cyclase)234 205174 s at QPCT glutaminyl peptide NM_012413 g9257235 NP_036545 Chr: 2p22.3 2.74008 4.436744 cyclotransferase (glutaminyl cyclase) /// glutaminyl-peptide cyclotransferase (glutaminyl cyclase)

235 221539 at EIF4EBP1 eukaryotic translation Initiation AB044548 gl 1527778 NPJ304086 Chr.8p12 2.73954 12.53374 factor 4E binding protein 1 /// eukaryotic translation initiation factor 4E binding protein 1235 221539 at EIF4EBP1 eukaryotic translation initiation AB044548 gl 1527778 NPJ304086 Chr.8p12 2.73954 12.53374 factor 4E binding protein 1 /// eukaryotic translation initiation factor 4E binding protein 1

236 211048 s at ERP70 protein disulfide isomerase BC006344 gl 3623480 NP_004902 Chr:7q35 2.73784 0.625663 related protein (calcium-binding protein, intestinal-related) /// protein disulfide isomerase related protein (calcium-binding protein, intestinal-related)236 211048 s at ERP70 protein disulfide isomerase BC006344 gl 3623480 NP_004902 Chr: 7q35 2.73784 0.625663 related protein (calcium-binding protein, intestinal-related) /// protein disulfide isomerase related protein (calcium-binding protein, intestinal-related)

237 209627_ s at OSBPL3 oxysterol binding protein-like 3 AY008372 gl 0880972 NP 056365 Chr:7p15 2 .73648 5.685182237 209627_ s at OSBPL3 oxysterol binding protein-like 3 AY008372 gl 0880972 NP 056365 Chr: 7p15 2 .73648 5.685182

238 225655. .at UHRF1 ubiquitin-like, containing PHD AK025578 Hs.108106.0 NP_ _037414 Chr:19p13 .3 2 .73224 2.08989 and RING finger domains, 1 238 225655.at UHRF1 ubiquitin-like, containing PHD AK025578 Hs.108106.0 NP_ _037414 Chr: 19p13 .3 2 .73224 2.08989 and RING finger domains, 1

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

239 203007_x_at LYPLA1 lysophospholipase I AF077198 g4679009 NP 006321 Chr:8q 11.23 2.73114 6.991358239 203007_x_at LYPLA1 lysophospholipase I AF077198 g4679009 NP 006321 Chr: 8q 11.23 2.73114 6.991358

240 230173_at Hs.65736.2 2.73028 4.174189240 230173_at Hs.65736.2 2.73028 4.174189

241 206102_at K1AA0186 KIAA0186 gene product NM_021067 gl 0800147 — Chr:20p11.21 2.72808 11.06357241 206102_at K1AA0186 KIAA0186 product NM_021067 gl 0800147 - Chr: 20p11.21 2.72808 11.06357

242 212624 s at CHN1 chimerin (chimaerin) 1 BF339445 Hs.169965.1 NP 001813 Chr:2q31-q32.1 2.7246 14.22309242 212624 s at CHN1 chimerin 1 BF339445 Hs.169965.1 NP 001813 Chr: 2q31-q32.1 2.7246 14.22309

243 205455 at MST1R macrophage stimulating 1 NMJD02447 g4505264 NP_002438 Chr:3p21.3 2.72337 1.702877 receptor (c-met-related tyrosine kinase) /// macrophage stimulating 1 receptor (c-met- related tyrosine kinase)243 205455 at MST1R macrophage stimulating 1 NMJD02447 g4505264 NP_002438 Chr: 3p21.3 2.72337 1.702877 receptor (c-met-related tyrosine kinase) /// macrophage stimulating 1 receptor (c-met-related tyrosine kinase)

244 225436_at LOC58489 hypothetical protein from AI339710 Hs.26765.0 — Chr:15q24.3 2.71839 2.08989 EUROIMAGE 588495244 225436_at LOC58489 hypothetical protein from AI339710 Hs.26765.0 - Chr: 15q24.3 2.71839 2.08989 EUROIMAGE 588495

245 205214_at STK17B serine/threonine kinase 17b NM_004226 g4758193 NP_004217 Chr:2q33.1 2.70435 8.153992 (apoptosis-inducing) /// serine/threonine kinase 17b (apoptosis-inducing)245 205214_at STK17B serine / threonine kinase 17b NM_004226 g4758193 NP_004217 Chr: 2q33.1 2.70435 8.153992 (apoptosis-inducing) /// serine / threonine kinase 17b (apoptosis-inducing)

246 219990_at FLJ23311 FLJ23311 protein NM_024680 gl 3375949 NP 078956 Chr:11p15.1 2.70342 4.149813246 219990_at FLJ23311 FLJ23311 protein NM_024680 gl 3375949 NP 078956 Chr: 11p15.1 2.70342 4.149813

247 209398 at HIST1H1C histone 1, H1c /// histone 1 , H1c BC002649 gl 2803628 NP 005310 Chr:6p21.3 2.70038 3.306454 g247 209398 at HIST1H1C histone 1, H1c /// histone 1, H1c BC002649 gl 2803628 NP 005310 Chr: 6p21.3 2.70038 3.306454 g

248 200747_s_at NUMA1 nuclear mitotic apparatus NM_006185 g5453819 NP_006176 Chr:11q13 2.69411 1.223189 protein 1 /// nuclear mitotic apparatus protein 1248 200747_s_a NUMA1 nuclear mitotic apparatus NM_006185 g5453819 NP_006176 Chr: 11q13 2.69411 1.223189 protein 1 /// nuclear mitotic apparatus protein 1

249 219014_at PLAC8 placenta-specific 8 NM_016619 g7706157 NP 057703 Chr:4q21.3 2.68216 3.612633249 219014_at PLAC8 placenta-specific 8 NM_016619 g7706157 NP 057703 Chr: 4q21.3 2.68216 3.612633

250 229645_at Similar to hypothetical protein H14197 Hs.227699.0 Chr:18q22.3 2.66618 0.149911 B230399E16 (LOC125704), mRNA250 229645_at Similar to hypothetical protein H14197 Hs.227699.0 Chr: 18q22.3 2.66618 0.149911 B230399E16 (LOC125704), mRNA

251 214590_s_at UBE2D1 ubiquitin-conjugating enzyme AL545760 Hs.318435.0 NP_003329 Chr:10q11.2- 2.66479 6.991358 E2D 1 (UBC4/5 homolog, q21 yeast)251 214590_s_at UBE2D1 ubiquitin-conjugating enzyme AL545760 Hs.318435.0 NP_003329 Chr: 10q11.2- 2.66479 6.991358 E2D 1 (UBC4 / 5 homologue, q21 yeast)

252 65438_at KIAA1609 KIAA1609 protein AA195124 4863696 RC NP_065998 Chr:16q24.1 2.66437 7.646445252 65438_at KIAA1609 KIAA1609 protein AA195124 4863696 RC NP_065998 Chr: 16q24.1 2.66437 7.646445

253 229500_at SLC30A9 solute carrierfamily 30 (zinc AI609256 Hs.270956.1 NP_006336 Chr:4p13-p12 2.65646 12.80219 transporter), member 9253 229500_at SLC30A9 solute carrierfamily 30 (zinc AI609256 Hs.270956.1 NP_006336 Chr: 4p13-p12 2.65646 12.80219 transporter), member 9

254 218468_s_at GREM1 gremiin 1 homolog, cysteine AF154054 gl 0863087 NP_037504 Chr:15q13-q15 2.63581 9.625715 knot superfamily (Xenopus laevis)254 218468_s_at GREM1 gremiin 1 homologue, cysteine AF154054 gl 0863087 NP_037504 Chr: 15q13-q15 2.63581 9.625715 knot superfamily (Xenopus laevis)

255 227350_at — CDNA FLJ11381 fis, clone AI889959 Hs.127797.0 — — 2.63429 12.80219 HEMBA1000501 255 227350_at - CDNA FLJ11381 fis, clone AI889959 Hs.127797.0 - - 2.63429 12.80219 HEMBA1000501

Nr. Affymetrix Gensymbol Genπame Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

256 204641_at NEK2 NIMA (never in mitosis gene a)- NM_002497 g4505372 NP_002488 Chr:1q32.2-q41 2.6329 13.40329 related kinase 2 /// NIMA (never in mitosis gene a)-related kinase 2256 204641_at NEK2 NIMA (never in mitosis gene a) - NM_002497 g4505372 NP_002488 Chr: 1q32.2-q41 2.6329 13.40329 related kinase 2 /// NIMA (never in mitosis gene a) -related kinase 2

257 221666_s_at ASC apoptosis-associated speck-like BC004470 gl 3325315 NP_037390 Chr:16p12- 2.62721 6.225758 protein containing a CARD /// p11.2 apoptosis-associated speck-like protein containing a CARD257 221666_s_at ASC apoptosis-associated speck-like BC004470 gl 3325315 NP_037390 Chr: 16p12- 2.62721 6.225758 protein containing a CARD /// p11.2 apoptosis-associated speck-like protein containing a CARD

258 202708_s_at HIST2H2BE histone 2, H2be /// histone 2, NM_003528 g4504276 NP_003519 Chr:1q21-q23 2.6179 11.06357 H2be258 202708_s_at HIST2H2BE histone 2, H2be /// histone 2, NM_003528 g4504276 NP_003519 Chr: 1q21-q23 2.6179 11.06357 H2be

259 209546_s_at APOL1 apolipoprotein L, 1 /// AF323540 g12408012 NP_003652 Chr:22q13.1 2.61762 4.174189 apolipoprotein L, 1259 209546_s_at APOL1 apolipoprotein L, 1 /// AF323540 g12408012 NP_003652 Chr: 22q13.1 2.61762 4.174189 apolipoprotein L, 1

260 218644 at PLEK2 pleckstrin 2 /// pleckstrin 2 NM 016445 g7706642 NP 057529 Chr:14q24.1 2.61324 3.612633260 218644 at PLEK2 pleckstrin 2 /// pleckstrin 2 NM 016445 g7706642 NP 057529 Chr: 14q24.1 2.61324 3.612633

261 214096_s_at SHMT2 serine AW190316 Hs.2186.4 NP_005403 Chr:12q12-q14 2.61188 8.779357 hydroxymethyltransferase 2 (mitochondrial)261 214096_s_at SHMT2 serine AW190316 Hs.2186.4 NP_005403 Chr: 12q12-q14 2.61188 8.779357 hydroxymethyltransferase 2 (mitochondrial)

262 221701_s_at FLJ 12541 stimulated by retinoic acid gene AF352728 gl 3560965 NP_071764 Chr:15q22.33 2.60779 2.571322 6 /// stimuiated by retinoic acid gene 6262 221701_s_at FLJ 12541 stimulated by retinoic acid gene AF352728 gl 3560965 NP_071764 Chr: 15q22.33 2.60779 2.571322 6 /// stimulated by retinoic acid gene 6

263 218706_s_at NS3TP2 HCV NS3-transactivated protein AW575493 Hs.235445.0 NP_076416 Chr:5q23.3 2.59546 10.20301 2263 218706_s_at NS3TP2 HCV NS3-transactivated protein AW575493 Hs.235445.0 NP_076416 Chr: 5q23.3 2.59546 10.20301 2

264 214463_x_at HIST1H4A histone 1 , H4a /// histone 1 , NM_003541 Hs.91031.0 NP_003529 Chr:6p21.3 2.59156 1.223189 H4a264 214463_x_at HIST1H4A histone 1, H4a /// histone 1, NM_003541 Hs.91031.0 NP_003529 Chr: 6p21.3 2.59156 1.223189 H4a

265 204033_at TRIP13 thyroid hormone receptor NM_004237 gl 1321606 NP_004228 Chr.5p15.33 2.59023 9.625715 interactor 13 /// thyroid hormone receptor interactor 13265 204033_at TRIP13 thyroid hormone receptor NM_004237 gl 1321606 NP_004228 Chr.5p15.33 2.59023 9.625715 interactor 13 /// thyroid hormone receptor interactor 13

266 200736_s_at GPX1 glutathione peroxidase 1 /// NM_000581 gl 0834975 NP_000572 Chr:3p21.3 2.58836 4.149813 glutathione peroxidase 1266 200736_s_at GPX1 glutathione peroxidase 1 /// NM_000581 gl 0834975 NP_000572 Chr: 3p21.3 2.58836 4.149813 glutathione peroxidase 1

267 225605 at LOC90313 hypothetical protein BC004507 AL540867 Hs.97871.0 NP 612358 Chr:17q11.2 2.58466 3.306454267 225605 at LOC90313 hypothetical protein BC004507 AL540867 Hs.97871.0 NP 612358 Chr: 17q11.2 2.58466 3.306454

268 204347 at AK3 adenylate kinase 3 AI653169 Hs.274691.0 NP 037542 Chr:1p31.3 2.58387 0.779487268 204347 at AK3 adenylate kinase 3 AI653169 Hs.274691.0 NP 037542 Chr: 1p31.3 2.58387 0.779487

269 214068_at BEAN brain expressed, associated AF070610 Hs.100543.0 — Chr:16q22.1 2.57926 11.06357 with Nedd4269 214068_at BEAN brain expressed, associated with AF070610 Hs.100543.0 - Chr: 16q22.1 2.57926 11.06357 with Nedd4

270 208583_x_at HIST1H2AJ histone 1 , H2aj /// histone 1 , NM_021066 gl 0800143 NP_066544 Chr:6p22-p21.3 2.57869 3.62324 H2aj270 208583_x_at HIST1H2AJ histone 1, H2aj /// histone 1, NM_021066 gl 0800143 NP_066544 Chr: 6p22-p21.3 2.57869 3.62324 H2aj

271 206307_s_at FOXD1 forkhead box D1 NMJ004472 g4758391 NP_004463 Chr:5q12-q13 2.56922 4.640066271 206307_s_at FOXD1 forkhead box D1 NMJ004472 g4758391 NP_004463 Chr: 5q12-q13 2.56922 4.640066

272 236535 at AW069285 Hs.159452.0 2.56884 4.640066 272 236535 at AW069285 Hs.159452.0 2.56884 4.640066

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

273 223631 _s_at C19orf33 chromosome 19 open reading AF213678 gl 2003430 NP_277055 Chr:19q13.13 2.56782 3.62324 frame 33273 223631 _s_at C19orf33 chromosome 19 open reading AF213678 gl 2003430 NP_277055 Chr: 19q13.13 2.56782 3.62324 frame 33

274 209581_at HRASLS3 HRAS-like suppressor 3 /// BC001387 gl 2655072 NP_009000 Chr:11q13.1 2.56741 8.779357 HRAS-like suppressor 3274 209581_at HRASLS3 HRAS-like suppressor 3 /// BC001387 gl 2655072 NP_009000 Chr: 11q13.1 2.56741 8.779357 HRAS-like suppressor 3

275 236669_at — CDNA FLJ31431 fis, clone AI038054 Hs.132890.0 — — 2.56492 2.08989 NT2NE2000548275 236669_at - CDNA FLJ31431 fis, clone AI038054 Hs.132890.0 - - 2.56492 2.08989 NT2NE2000548

276 209478_at STRA13 stimulated by retinoic acid 13 U95006 g2071992 NP 659435 Chr:17q25.3 2.54406 6.991358276 209478_at STRA13 stimulated by retinoic acid 13 U95006 g2071992 NP 659435 Chr: 17q25.3 2.54406 6.991358

277 201136_at PLP2 proteoüpid protein 2 (colonic NMJ302668 g4505892 NP_002659 Chr:xp11.23 2.5406 2.571322 epithelium-enriched) /// proteolipid protein 2 (colonic epithelium-enriched)277 201136_at PLP2 protein protein 2 (colonic NMJ302668 g4505892 NP_002659 Chr: xp11.23 2.5406 2.571322 epithelium-enriched) /// proteolipid protein 2 (colonic epithelium-enriched)

278 214522_x__at HIST1H3D histone 1 , H3d /// histone 1 , NM_021065 Hs.239458.0 NP_003521 Chr:6p21.3 2.53255 3.62324 H3d278 214522_x__at HIST1H3D histone 1, H3d /// histone 1, NM_021065 Hs.239458.0 NP_003521 Chr: 6p21.3 2.53255 3.62324 H3d

279 227998_at S100A16 S 100 calcium binding protein AA045184 Hs.738.3 NP_525127 — 2.52659 9.840216 A16279 227998_at S100A16 S 100 calcium binding protein AA045184 Hs.738.3 NP_525127 - 2.52659 9.840216 A16

280 215629_s__at DLEU2 deleted in lymphocytic AA905286 Hs.306636.0 NP_006012 Chr:13q14.3 2.51998 6.225758 leukemia, 2280 215629_s__at DLEU2 deleted in lymphocytic AA905286 Hs.306636.0 NP_006012 Chr: 13q14.3 2.51998 6.225758 leukemia, 2

281 204444_at KIF11 kinesin family member 11 /// NMJD04523 gl 3699823 NP_004514 Chr:10q24.1 2.51811 4.436744 kinesin family member 11281 204444_at KIF11 kinesin family member 11 /// NMJD04523 gl 3699823 NP_004514 Chr: 10q24.1 2.51811 4.436744 kinesin family member 11

282 204240_s_at SMC2L1 SMC2 structural maintenance NM_006444 g5453590 NP_006435 Chr:9q31.2 2.49867 8.153992 of chromosomes 2-like 1 (yeast)282 204240_s_at SMC2L1 SMC2 structural maintenance NM_006444 g5453590 NP_006435 Chr: 9q31.2 2.49867 8.153992 of chromosomes 2-like 1 (yeast)

283 206247_at MICB MHC class I polypeptide-related NM_005931 g5174564 NP_005922 Chr:6p21.3 2.49468 3.612633 sequence B /// MHC class I polypeptide-related sequence B283 206247_at MICB MHC class I polypeptide-related NM_005931 g5174564 NP_005922 Chr: 6p21.3 2.49468 3.612633 sequence B /// MHC class I polypeptide-related sequence B

284 209806_at HIST1H2BE histone 1 , H2be /// histone 1 , BC000893 gl 2654150 NP_003514 Chr:6p21.3 2.48301 0.779487 H2be284 209806_at HIST1H2BE histone 1, H2be /// histone 1, BC000893 gl 2654150 NP_003514 Chr: 6p21.3 2.48301 0.779487 H2be

285 219559_at C20orf59 chromosome 20 open reading NM_022082 gl 1545794 NP_071365 Chr:20q13.33 2.47821 3.306454 frame 59285 219559_at C20orf59 chromosomes 20 open reading NM_022082 gl 1545794 NP_071365 Chr: 20q13.33 2.47821 3.306454 frame 59

286 205339_at SIL TAL1 (SCL) interrupting locus NM_003035 g4506958 NP_003026 Chr:1p32 2.47667 1.223189 /// TAL1 (SCL) interrupting locus286 205339_at SIL TAL1 (SCL) interrupting locus NM_003035 g4506958 NP_003026 Chr: 1p32 2.47667 1.223189 /// TAL1 (SCL) interrupting locus

287 209507_at RPA3 replication protein A3, 14kDa /// BC005264 gl 3528944 NP_002938 Chr:7p22 2.4764 1.578435 replication protein A3, 14kDa287 209507_at RPA3 replication protein A3, 14kDa /// BC005264 gl 3528944 NP_002938 Chr: 7p22 2.4764 1.578435 replication protein A3, 14kDa

288 225799_at MGC4677 hypothetical protein MGC4677 BF209337 Hs.50724.0 NP 443103 Chr:2p11.2 2.47527 13.64212288 225799_at MGC4677 hypothetical protein MGC4677 BF209337 Hs.50724.0 NP 443103 Chr: 2p11.2 2.47527 13.64212

289 241872_at — Transcribed sequences AI149963 Hs.132121.0 — — 2.46984 3.306454 289 241872_at - Transcribed sequences AI149963 Hs.132121.0 - - 2.46984 3.306454

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valυe Probennummer ID Protein ID Lokalisierung Change {%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-valυe Sample Number ID Protein ID Localization Change {%)

290 207202_s_at NR1I2 nuclear receptor subfamiiy 1, NM_003889 gl 1863133 NP_003880 Chr:3q12-q13.3 2.46766 12.53374 group I, member 2 /// nuclear receptor subfamiiy 1, group I1 member 2290 207202_s_at NR1I2 nuclear receptor subfamiiy 1, NM_003889 gl 1863133 NP_003880 Chr: 3q12-q13.3 2.46766 12.53374 group I, member 2 /// nuclear receptor subfamiiy 1, group I 1 member 2

291 224428_s_at CDCA7 cell division cycle associated 7 AY029179 gl 3641303 NPJ 14148 Chr:2q31 2.46745 11.55252 /// cell division cycle associated 7291 224428_s_at CDCA7 cell division cycle associated 7 AY029179 gl 3641303 NPJ 14148 Chr: 2q31 2.46745 11.55252 /// cell division cycle associated 7

292 229860 x at — Clone IMAGE-.5200861, mRNA AI341602 Hs.152932.0 — Chr:4p16.3 2.46209 12.80219292 229860 x at - Clone IMAGE-.5200861, mRNA AI341602 Hs.152932.0 - Chr: 4p16.3 2.46209 12.80219

293 239049 at — — BF514509 Hs.262571.0 — — 2.46107 0.518167293 239049 at - - BF514509 Hs.262571.0 - - 2.46107 0.518167

294 209891 at Spc25 kinetochore protein Spc25 AF225416 g9963834 NP 065726 Chr:2q31.1 2.44983 3.612633294 209891 at Spc25 kinetochore protein Spc25 AF225416 g9963834 NP 065726 Chr: 2q31.1 2.44983 3.612633

295 222750 s at FU13352 hypothetica! protein FLJ13352 BC002480 gl 2803324 NP 078868 Chr:4q12 2.44845 12.80219295 222750 s at FU13352 hypothetica! protein FLJ13352 BC002480 gl 2803324 NP 078868 Chr: 4q12 2.44845 12.80219

296 2Q4702_s_at NFE2L3 nuclear factor (erythroid-derived NM_004289 g5731346 NP__004280 Chr:7p15-p14 2.44496 3.612633 2)-like 3 /// nuclear factor (erythroid-derived 2)-like 3296 2Q4702_s_at NFE2L3 nuclear factor (erythroid-derived NM_004289 g5731346 NP__004280 Chr: 7p15-p14 2.44496 3.612633 2) -like 3 /// nuclear factor (erythroid-derived 2) -like 3

297 208688_x_at EIF3S9 eukaryotic translation initiation U78525 g2558667 NP_003742 Chr:7p22.3 2.44332 4.149813 factor 3, subunit 9 eta, 116kDa297 208688_x_at EIF3S9 eukaryotic translation initiation U78525 g2558667 NP_003742 Chr: 7p22.3 2.44332 4.149813 factor 3, subunit 9 eta, 116kDa

298 202483_s_at RANBP1 RAN binding protein 1 /// RAN NM_002882 g6382077 NPJ302873 Chr:22q11.21 2.44314 1.223189 binding protein 1 UJ298 202483_s_at RANBP1 RAN binding protein 1 /// RAN NM_002882 g6382077 NPJ302873 Chr: 22q11.21 2.44314 1.223189 binding protein 1 UJ

299 204769 s at TAP2 transporter 2, ATP-binding M74447 gl 88505 NP_000535 Chr:6p21.3 2.44046 10.20301 cassette, sub-family B (MDR/TAP) /// transporter 2, ATP-binding cassette, sub- family B (MDR/TAP)299 204769 s at TAP2 transporter 2, ATP-binding M74447 gl 88505 NP_000535 Chr: 6p21.3 2.44046 10.20301 cassette, sub-family B (MDR / TAP) /// transporter 2, ATP-binding cassette, subfamily B (MDR / TAP)

300 226452. _at PDK1 pyruvate dehydrogenase AU146532 Hs.61712.1 NP. „002601 Chr.2q31.1 2.43867 1.702877 kinase, isoenzyme 1300 226452. _at PDK1 pyruvate dehydrogenase AU146532 Hs.61712.1 NP. " 002601 Chr.2q31.1 2.43867 1.702877 kinase, isoenzymes 1

301 221730. _at COL5A2 Collagen, type V, alpha 2 NM_000393 Hs.82985.0 NP. _000384 Chr:2q14-q32 2.43679 8.153992301 221730. _at COL5A2 Collagen, type V, alpha 2 NM_000393 Hs.82985.0 NP . _000384 Chr: 2q14-q32 2.43679 8.153992

302 200619. _at SF3B2 splicing factor 3b, subunit 2, NM_006842 g5803154 Chr:11q13.1 2.43447 3.612633 145kDa /// splicing factor 3b, subunit 2, 145kDa302 200619. _at SF3B2 splicing factor 3b, subunit 2, NM_006842 g5803154 Chr: 11q13.1 2.43447 3.612633 145kDa /// splicing factor 3b, subunit 2, 145kDa

303 203744. _at HMGB3 high-mobility group box 3 /// NM_005342 g4885420 NP. .005333 Chr:xq28 2.43432 12.53374 high-mobiüty group box 3303 203744. _at HMGB3 high-mobility group box 3 /// NM_005342 g4885420 NP. .005333 Chr: xq28 2.43432 12.53374 high-mobiüty group box 3

304 204971. _at CSTA cystatin A (stefin A) /// cystatin NMJD05213 g4885164 NP. .005204 Chr:3q21 2.43141 4.640066 A (stefin A) 304 204971. _at CSTA cystatin A (stefin A) /// cystatin NMJD05213 g4885164 NP. .005204 Chr: 3q21 2.43141 4.640066 A (stefin A)

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

305 205590_at RASGRP1 RAS guanyl releasing protein 1 NM_005739 g6382080 NPJ305730 Chr:15q15 2.42477 14.22309 (calcium and DAG-regulated) /// RAS guanyl releasing protein 1 (calcium and DAG-regulated)305 205590_at RASGRP1 RAS guanyl releasing protein 1 NM_005739 g6382080 NPJ305730 Chr: 15q15 2.42477 14.22309 (calcium and DAG-regulated) /// RAS guanyl releasing protein 1 (calcium and DAG-regulated)

306 218840 s at NADSYN 1 NAD synthetase 1 NM 018161 g8922560 NP 060631 Chr:11q13.2 2.42354 11.70204306 218840 s at NADSYN 1 NAD synthetase 1 NM 018161 g8922560 NP 060631 Chr: 11q13.2 2.42354 11.70204

307 221648 s at PNAS-4 CGI-146 protein AK025651 Hs.42409.1 NP 057160 Chr:1q44 2.42293 3.62324307 221648 s at PNAS-4 CGI-146 protein AK025651 Hs.42409.1 NP 057160 Chr: 1q44 2.42293 3.62324

308 216237_s_at MCM5 MCM5 minichromosome AA807529 Hs.77171.1 NP_006730 Chr:22q13.1 2.42147 2.571322 maintenance deficient 5, cell division cycle 46 (S. cerevisiae)308 216237_s_at MCM5 MCM5 minichromosomes AA807529 Hs.77171.1 NP_006730 Chr: 22q13.1 2.42147 2.571322 maintenance deficient 5, cell division cycle 46 (S. cerevisiae)

309 203892_at g5174454 2.41334 4.436744309 203892_at g5174454 2.41334 4.436744

310 214542_x_at HIST1H3H histone 1 , H3h /// histone 1 , NM_003509 Hs.137592.0 NPJ303527 Chr:6p22-p21.3 2.4095 2.814919 H3h310 214542_x_at HIST1H3H histone 1, H3h /// histone 1, NM_003509 Hs.137592.0 NPJ303527 Chr: 6p22-p21.3 2.4095 2.814919 H3h

311 218951 s at FLJ11323 hypothetical protein FLJ11323 NM 018390 g8922994 Chr:xp22.33 2.40548 13.40329311 218951 s at FLJ11323 hypothetical protein FLJ11323 NM 018390 g8922994 Chr: xp22.33 2.40548 13.40329

312 202307_s_at TAP1 transporter 1 , ATP-binding NM_000593 g9665247 NP_000584 Chr:6p21.3 2.40488 1.578435 cassette, sub-family B (MDR/TAP)312 202307_s_at TAP1 transporter 1, ATP-binding NM_000593 g9665247 NP_000584 Chr: 6p21.3 2.40488 1.578435 cassette, sub-family B (MDR / TAP)

313 202934 at HK2 hexokinase 2 AI761561 Hs.198427.0 NP 000180 Chr:2p13 2.40302 0.353669313 202934 at HK2 hexokinase 2 AI761561 Hs.198427.0 NP 000180 Chr: 2p13 2.40302 0.353669

314 238021_s_at — LOC388279 (LOC388279), AA954994 Hs.237396.1 — Chr:16q12.2 2.40174 0.779487 mRNA314 238021_s_at - LOC388279 (LOC388279), AA954994 Hs.237396.1 - Chr: 16q12.2 2.40174 0.779487 mRNA

315 214061 at MGC21654 unknown MGC21654 product AI017564 Hs .95631.0 ^NP_663622 Chr:8q24.13 2.39907 8.153992MGC21654 product AI017564 Hs .95631.0 ^ NP_663622 Chr: 8q24.13 2.39907 8.153992

316 219773_at NOX4 NADPH oxidase 4 /// NADPH NM_016931 g8393842 NPJD58627 Chr:11q14.2- 2.39385 11.70204 oxidase 4 q21316 219773_at NOX4 NADPH oxidase 4 /// NADPH NM_016931 g8393842 NPJD58627 Chr: 11q14.2- 2.39385 11.70204 oxidase 4 q21

317 207222_at PLA2G10 phospholipase A2, group X /// NM_003561 g4505844 NP_003552~ • Chr:16p13.1~ 2.39346 8.779357 phospholipase A2, group X p12317222_at PLA2G10 phospholipase A2, group X /// NM_003561 g4505844 NP_003552 ~ • Chr: 16p13.1 ~ 2.39346 8.779357 phospholipase A2, group X p12

318 218039_at NUSAP1 nucleolar and spindle NM_016359 g7705950 NP_057443 Chr:15q14 2.3908 8.153992 associated protein 1 /// nucleolar and spindle associated protein 1318 218039_at NUSAP1 nucleolar and spindle NM_016359 g7705950 NP_057443 Chr: 15q14 2.3908 8.153992 associated protein 1 /// nucleolar and spindle associated protein 1

319 230398_at CTEN C-terminal tensin-like AA158731 Hs.165544.0 NP 116254 Chr:17q21.2 2.3895 0.625663319 230398_at CTEN C-terminal tensin-like AA158731 Hs.165544.0 NP 116254 Chr: 17q21.2 2.3895 0.625663

320 37424_at C6orf18 chromosome 6 open reading AB029343 4850640 NPJ361925 Chr:6p21.3 2.38743 3.61384 frame 18320 37424_at C6orf18 chromosomes 6 open reading AB029343 4850640 NPJ361925 Chr: 6p21.3 2.38743 3.61384 frame 18

321 223779_at MGC10981 hypothetical protein MGC10981 BC004397 gl 3325159 — Chr:4p16.1 2.38604 1.380569321 223779_at MGC10981 hypothetical protein MGC10981 BC004397 gl 3325159 - Chr: 4p16.1 2.38604 1.380569

322 239384_at SFRS1 splicing factor, arginine/serine- R18746 Hs.36747.0 NP_008855 Chr:17q21.3- 2.38021 0.353669 rich 1 (splicing factor 2, q22 alternate splicing factor) 322 239384_at SFRS1 splicing factor, arginine / serine R18746 Hs.36747.0 NP_008855 Chr: 17q21.3- 2.38021 0.353669 rich 1 (splicing factor 2, q22 alternate splicing factor)

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

323 210830_s_at PON2 paraoxonase 2 AF001602 g2228776 NP 000296 Chr:7q21.3 2.37738 4.830969323 210830_s_at PON2 paraoxonase 2 AF001602 g2228776 NP 000296 Chr: 7q21.3 2.37738 4.830969

324 235113_at PPIL5 peptidylprolyl isomerase AA742244 Hs.179805.0 NP_689542 Chr:14q21.3 2.37718 1.380569 (cyclophilin) like 5324 235113_at PPIL5 peptidylprolyl isomerase AA742244 Hs.179805.0 NP_689542 Chr: 14q21.3 2.37718 1.380569 (cyclophilin) like 5

325 214806_at BICD1 Bicaudal D homolog 1 U90030 Hs.164975.2 NP_001705 Chr:12p11.2- 2.37514 4.436744 (Drosophila) /// Bicaudal D p11.1 homolog 1 (Drosophila)325 214806_at BICD1 Bicaudal D homologue 1 U90030 Hs.164975.2 NP_001705 Chr: 12p11.2- 2.37514 4.436744 (Drosophila) /// Bicaudal D p11.1 homolog 1 (Drosophila)

326 226630_at C14orf106 chromosome 14 open reading BF062175 Hs.89278.1 NP_060823 Chr:14q21.3 2.37019 4.640066 frame 106326 226630_at C14orf106 chromosome 14 open reading BF062175 Hs.89278.1 NP_060823 Chr: 14q21.3 2.37019 4.640066 frame 106

327 223484_at NMES1 normal mucosa of esophagus AF228422 gl 2656020 NP_115789 Chr:15q15.1 2.36662 2.814919 specific 1327 223484_at NMES1 normal mucosa of esophagus AF228422 gl 2656020 NP_115789 Chr: 15q15.1 2.36662 2.814919 specific 1

328 228143 at CP ceruloplasmin (ferroxidase) AI684991 Hs.282804.0 NP 000087 Chr:3q23-q25 2.36183 2.08989328 228143 at CP ceruloplasmin (ferroxidase) AI684991 Hs.282804.0 NP 000087 Chr: 3q23-q25 2.36183 2.08989

329 201671_x_at USP14 ubiquitin specific protease 14 BC003556 gl 3097695 NP_005142 Chr:18p11.32 2.35987 8.779357 (tRNA-guanine transglycosylase)329 201671_x_at USP14 ubiquitin specific protease 14 BC003556 gl 3097695 NP_005142 Chr: 18p11.32 2.35987 8.779357 (tRNA guanine transglycosylase)

330 201201_at CSTB cystatin B (stefin B) /// cystatin NM_000100 g4503116 NP_000091 Chr:21q22.3 2.35349 12.53374 B (stefin B)330 201201_at CSTB cystatin B (stefin B) /// cystatin NM_000100 g4503116 NP_000091 Chr: 21q22.3 2.35349 12.53374 B (stefin B)

331 230252 at GPR92 G protein-coupled receptor 92 AW183080 Hs.155538.0 NP 065133 Chr:12p13.31 2.35144 4.640066331 230252 at GPR92 G protein-coupled receptor 92 AW183080 Hs.155538.0 NP 065133 Chr: 12p13.31 2.35144 4.640066

332 217728_at S100A6 S100 calcium binding protein NM_014624 g9845517 NP_055439 Chr:1q21 2.34487 1.901012 w A6 (calcyclin) /// S100 calcium binding protein A6 (calcyclin)332 217728_at S100A6 S100 calcium binding protein NM_014624 g9845517 NP_055439 Chr: 1q21 2.34487 1.901012 w A6 (calcycline) /// S100 calcium binding protein A6 (calcycline)

333 219901_at FGD6 FYVE, RhoGEF and PH domain NM_018351 g8922920 — Chr:12q23.1 2.33964 3.306454 containing 6333 219901_at FGD6 FYVE, RhoGEF and PH domain NM_018351 g8922920 - Chr: 12q23.1 2.33964 3.306454 containing 6

334 202737_s_at LSM4 LSM4 homolog, U6 small NM_012321 g6912485 NP_036453 Chr:19p13.11 2.33857 3.612633 nuclear RNA associated (S. cerevisiae)334 202737_s_at LSM4 LSM4 homologue, U6 small NM_012321 g6912485 NP_036453 Chr: 19p13.11 2.33857 3.612633 nuclear RNA associated (S. cerevisiae)

335 222646_s_at ERO1L ERO1-Iike (S. cerevisiae) AW268365 Hs.25740.0 NP 055399 Chr:14q22.1 2.33385 1.380569335 222646_s_at ERO1L ERO1-Iike (S. cerevisiae) AW268365 Hs.25740.0 NP 055399 Chr: 14q22.1 2.33385 1.380569

336 203064_s_at FOXK2 forkhead box K2 /// forkhead NM_004514 g4758599 NP_004505 Chr:17q25 2.33331 3.61384 box K2336 203064_s_at FOXK2 forkhead box K2 /// forkhead NM_004514 g4758599 NP_004505 Chr: 17q25 2.33331 3.61384 box K2

337 213288_at LOC129642 hypothetical protein BC016005 AI761250 Hs.90797.0 NP 620154 Chr:2p25.2 2.33183 11.70204337 213288_at LOC129642 hypothetical protein BC016005 AI761250 Hs.90797.0 NP 620154 Chr: 2p25.2 2.33183 11.70204

338 47105_at FLJ20399 hypothetical protein FLJ20399 AA886893 4859973 RC NP 060273 Chr:16q22.1 2.33175 0.095261338 47105_at FLJ20399 hypothetical protein FLJ20399 AA886893 4859973 RC NP 060273 Chr: 16q22.1 2.33175 0.095261

339 210201_x_at BIN1 bridging integrator 1 AF001383 g2199534 NP 004296 Chr:2q14 2.33163 9.625715339 210201_x_at BIN1 bridging integrator 1 AF001383 g2199534 NP 004296 Chr: 2q14 2.33163 9.625715

340 230940_at TCEB3BP1 transcription elongation factor B AI653385 Hs.98190.0 NP_065746 Chr:19p13.3 2.3307 2.814919 Polypeptide 3 binding protein 1 340 230940_at TCEB3BP1 transcription elongation factor B AI653385 Hs.98190.0 NP_065746 Chr: 19p13.3 2.3307 2.814919 Polypeptides 3 binding protein 1

Nr, Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valυeNr, Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-valve

Probennummer ID Protein !D Lokalisierung Change (%)Sample Number ID Protein! D Localization Change (%)

341 207543 s at P4HA1 procollagen-proiine, 2- NM 000917 g4505564 NP 000908 Chr:10q21.3- 2.3304 4.830969 oxoglutarate 4-dioxygenase q23.1 (proline 4-hydroxylase), alpha Polypeptide I /// procoüagen- proline, 2-oxoglutarate 4- dioxygenase (proline 4- hydroxylase), alpha Polypeptide341 207543 s at P4HA1 procollagen-proiine, 2 -NM 000917 g4505564 NP 000908 Chr: 10q21.3- 2.3304 4.830969 oxoglutarate 4-dioxygenase q23.1 (proline 4-hydroxylase), alpha polypeptides I /// procoagulpoprolines, 2- oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptides

II

342 238807, _at LOC157567 hypothetical protein AW973964 Hs.291531.0 NP. _940683 Chr:8q22.3 2.31789 1.901012 LOC157567342 238807 , _at LOC157567 hypothetical protein AW973964 Hs.291531.0 NP. _940683 Chr: 8q22.3 2.31789 1.901012 LOC157567

343 209604. _s_at GATA3 GATA binding protein 3 /// BC003070 gl 3111765 NP. J3Q2042 Chr.10p15 2.31774 13.40329 GATA binding protein 3343 209604. _s_at GATA3 GATA binding protein 3 /// BC003070 gl 3111765 NP. J3Q2042 Chr.10p15 2.31774 13.40329 GATA binding protein 3

344 201762 s at PSME2 proteasome (prosome, NMJ3Q2818 g4506236 NPJ302809 Chr:14q11.2 2.31359 0.095261 ' macropain) activator sυbυnit 2 (PA28 beta) /// proteasome (prosome, macropain} activator subunit 2 (PA28 beta) κ>Proteaseome (prosome, NMJ3Q2818 g4506236 NPJ302809 Chr: 14q11.2 2.31359 0.095261 'macropain) activator with 2 (PA28 beta) /// proteasome (prosome, macropain} activator subunit 2 (PA28 beta) κ>

345 218986 s at NM 017631 g8923037 2.31258 1.702877345 218986 s at NM 017631 g8923037 2.31258 1.702877

346 210933_s_at MGC4655 hypothetical protein MGC4655 BC004908 g13436196 NPJ 71608 Chr:16q22.1 2.30895 2.814919346 210933_s_at MGC4655 hypothetical protein MGC4655 BC004908 g13436196 NPJ 71608 Chr: 16q22.1 2.30895 2.814919

347 208758 at ATIC 5-aminoimidazole-4- D89976 g2317691 NP_004035 Chr:2q35 2.30776 1.578435 carboxarnide riboπucleotide formyltransferase/IMP cyclohydrolase /// 5- aminoimidazoie-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase347 208758 ATIC 5-aminoimidazole-4- D89976 g2317691 NP_004035 Chr: 2q35 2.30776 1.578435 carboxamide ribotrenucleotide formyltransferase / IMP cyclohydrolase /// 5-aminoimidazoie-4-carboxamide ribonucleotide formyltransferase / IMP cyclohydrolase

348 205266 at LlF leukemia inhibitory factor NM_002309 g6006018 NP„002300 Chr:22q12.2 2.30602 14.22309 (cholinergic differentiation factor) /// leukemia inhibitory factor (cholinergic differentiation factor)348 205266 at LlF leukemia inhibitory factor NM_002309 g6006018 NP " 002300 Chr: 22q12.2 2.30602 14.22309 (cholinergic differentiation factor) /// leukemia inhibitory factor (cholinergic differentiation factor)

349 204162 at KNTC2 kinetochore associated 2 /// NM 006101 g5174456 NP_006092 Chr:18p11.31 2.30491 7.646445 kinetochore associated 2 349 204162 at KNTC2 kinetochore associated 2 /// NM 006101 g5174456 NP_006092 Chr: 18p11.31 2.30491 7.646445 kinetochore associated 2

Nr. Affymetrix Gensymbol Geπname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change '0/-Sample ID ID Protein ID Localization Change ' 0 / -

350 200640 at YWHAZ tyrosine 3- NM 003406 g4507952 NP_003397 Chr:8q23.1 2.30049 3.612633 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta Polypeptide /// tyrosine 3- monooxygenase/tryptophan 5 monooxygenase activation protein, zeta Polypeptide350 200640 at YWHAZ tyrosine 3-NM 003406 g4507952 NP_003397 Chr: 8q23.1 2.30049 3.612633 monooxygenase / tryptophan 5 monooxygenase activating protein, zeta polypeptides /// tyrosine 3-monooxygenase / tryptophan 5 monooxygenase activating protein, zeta polypeptides

351 218961_s_at PNKP polynucleotide kinase 3'- NM_007254 g6005835 NP_009185 Chr:19q13.3- 2.2928 12.53374 phosphatase /// polynucleotide q13.4 kinase 3'-phosphatase351 218961_s_at PNKP polynucleotide kinase 3'-NM_007254 g6005835 NP_009185 Chr: 19q13.3- 2.2928 12.53374 phosphatase /// polynucleotides q13.4 kinase 3'-phosphatase

352 210766_s_at CSE1L CSE1 chromosome segregation AF053640 g3560554 NP_001307 Chr:20q13 2.29189 3.612633 I-Iike (yeast)352 210766_s_at CSE1L CSE1 chromosomal segregation AF053640 g3560554 NP_001307 Chr: 20q13 2.29189 3.612633 I-Iike (yeast)

353 240531 _at — — AW268727 Hs.112805.0 — — 2.29057 2.814919353 240531 _at - - AW268727 Hs.112805.0 - - 2.29057 2.814919

354 229432_at NAGS N-acetylglutamate synthase AV696264 Hs.8876.0 NP 694551 Chr:17q21.31 2.28831 2.08989NAGS N-acetylglutamate synthase AV696264 Hs.8876.0 NP 694551 Chr: 17q21.31 2.28831 2.08989

355 221653_x_at APOL2 apolipoprotein L, 2 BC004395 gl 3325155 NP 112092 Chr:22q12 2.28719 4.640066355 221653_x_at APOL2 apolipoprotein L, 2 BC004395 gl 3325155 NP 112092 Chr: 22q12 2.28719 4.640066

356 224327_s_at DGAT2 diacylglycerol O-acyltransferase AB048286 gl 3537296 NPJ 15953 Chr:11q13.3 2.28711 1.051298 homolog 2 (mouse) /// diacylglycerol O-acyltransferase homolog 2 (mouse)356 224327_s_at DGAT2 diacylglycerol O-acyltransferase AB048286 gl 3537296 NPJ 15953 Chr: 11q13.3 2.28711 1.051298 homolog 2 (mouse) /// diacylglycerol O-acyltransferase homolog 2 (mouse)

357 202619_s_at PLOD2 procollagen-lysine, 2- AI754404 Hs.41270.0 NP_000926 Chr:3q23-q24 2.28553 3.61384 oxoglutarate 5-dioxygenase (lysine hydroxylase) 2357 202619_s_at PLOD2 procollagen lysines, 2- AI754404 Hs.41270.0 NP_000926 Chr: 3q23-q24 2.28553 3.61384 oxoglutarate 5-dioxygenase (lysine hydroxylase) 2

358 203789_s_at SEMA3C sema domain, Immunoglobulin NM_006379 g5454047 NP_006370 Chr:7q21-q31 2.28491 3.612633 domain (Ig), short basic domain, secreted, (semaphorin) 3C358 203789_s_at SEMA3C sema domain, immunoglobulin NM_006379 g 5454047 NP_006370 Chr: 7q21-q31 2.28491 3.612633 domain (Ig), short basic domain, secreted, (semaphorin) 3C

359 213007_at FLJ10719 hypothetical protein FLJ 10719 W74442 Hs.80961.1 NP_060663 Chr:15q25-q26 2.28448 1.578435359 213007_at FLJ10719 hypothetical protein FLJ 10719 W74442 Hs.80961.1 NP_060663 Chr: 15q25-q26 2.28448 1.578435

360 219546_at BMP2K BMP2 inducible kinase NM_017593 g9055205 NP 060063 Chr:4q21.23 2.27648 9.625715360 219546_at BMP2K BMP2 inducible kinase NM_017593 g9055205 NP 060063 Chr: 4q21.23 2.27648 9.625715

361 225777_at C9orf140 chromosome 9 open reading AW250904 Hs.19322.0 NP_848543 Chr:9q34.3 2.27646 1.901012 frame 140361 225777_at C9orf140 chromosomes 9 open reading AW250904 Hs.19322.0 NP_848543 Chr: 9q34.3 2.27646 1.901012 frame 140

362 244749_at — CDNA FLJ42484 fis, clone AA806349 Hs.44698.0 — -— 2.27526 11.55252 BRACE2032182 362 244749_at - CDNA FLJ42484 fis, clone AA806349 Hs.44698.0 - - 2.27526 11.55252 BRACE2032182

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

363 201577_at NME1 noπ-metastatic cells 1 , protein NM_000269 g4557796 NPJ300260 Chr:17q21.3 2.26917 1.578435 (NM23A) expressed in /// non- metastatic cells 1 , protein (NM23A) expressed in363 201577_at NME1 noπ-metastatic cells 1, protein NM_000269 g4557796 NPJ300260 Chr: 17q21.3 2.26917 1.578435 (NM23A) expressed in /// non-metastatic cells 1, protein (NM23A) expressed in

364 225944_at NLN neurolysin (metallopeptidase AW006938 Hs.22151.1 NP_065777 Chr:5q12.3 2.26733 11.55252 M3 family)364 225944_at NLN neurolysin (metallopeptidase AW006938 Hs.22151.1 NP_065777 Chr: 5q12.3 2.26733 11.55252 M3 family)

365 230165 at N31731 Hs.44269.0 2.26719 3.62324365 230165 at N31731 Hs.44269.0 2.26719 3.62324

366 204023_at RFC4 replicatioπ factor C (activator 1 ) NM_002916 g4506490 NPJ302907 Chr:3q27 2.26351 1.380569 4, 37kDa /// replication factor C (activator 1) 4, 37kDa366 204023_at RFC4 replicator factor C (activator 1) NM_002916 g4506490 NPJ302907 Chr: 3q27 2.26351 1.380569 4, 37kDa /// replication factor C (activator 1) 4, 37kDa

367 209173_at AGR2 anterior gradient 2 homolog AF088867 g6652811 NPJ306399 Chr:7p21.3 2.26177 11.06357 (Xenopus laevis) /// anterior gradient 2 homolog (Xenopus laevis)367 209173_at AGR2 anterior gradient 2 homologous AF088867 g6652811 NPJ306399 Chr: 7p21.3 2.26177 11.06357 (Xenopus laevis) /// anterior gradient 2 homologous (Xenopus laevis)

368 221903_s_at CYLD cyündromatosis (turban tumor BE046443 Hs.18827.0 NP_056062 Chr:16q12.1 2.26132 6.991358 Syndrome)368 221903_s_at CYLD cyutromatosis (turban tumor BE046443 Hs.18827.0 NP_056062 Chr: 16q12.1 2.26132 6.991358 Syndromes)

369 213355_at SIAT10 sialyltransferase 10 (alpha-2,3- AI989567 Hs.34578.2 NP_006091 Chr:3q12.2 2.26038 14.22309 sialyltransferase VI)369 213355_at SIAT10 sialyltransferase 10 (alpha-2,3-AI989567 Hs.34578.2 NP_006091 Chr: 3q12.2 2.26038 14.22309 sialyltransferase VI)

370 201587_s_at 1RAK1 interleukin-1 receptor- NM_001569 g4755143 NP_001560 Chr:xq28 2.2541 4.830969 » associated kinase 1 /// interleukin-1 receptor- associated kinase 1370 201587_s_at 1RAK1 interleukin-1 receptor NM_001569 g4755143 NP_001560 Chr: xq28 2.2541 4.830969 »associated kinase 1 /// interleukin-1 receptor-associated kinase 1

371 201417_at SOX4 SRY (sex determining region AL136179 Hs.83484.0 NP_003098 Chr:6p22.3 2.25311 3.612633 Y)-box 4371 201417_at SOX4 SRY (sex determining region AL136179 Hs.83484.0 NP_003098 BC: 6p22.3 2.25311 3.612633 Y) -box 4

372 238843 at NPHP1 nephroπophthisis 1 (juvenile) BF216535 Hs.211782.0 NP 000263 Chr:2q13 2.25157 1.380569372 238843 at NPHP1 nephroπophthisis 1 (juvenile) BF216535 Hs.211782.0 NP 000263 Chr: 2q13 2.25157 1.380569

373 207966 s at GLG1 golgi apparatus protein 1 NM 012201 g6912389 NP 036333 Chr:16q22-q23 2.24967 9.625715373 207966 s at GLG1 golgi apparatus protein 1 NM 012201 g6912389 NP 036333 Chr: 16q22-q23 2.24967 9.625715

374 215071 s at HIST1 H2AC histone 1 , H2ac AL353759 Hs.28777.3 NP 003503 Chr:6p21.3 2.24876 6.225758374 215071 s at HIST1 H2AC histone 1, H2ac AL353759 Hs.28777.3 NP 003503 Chr: 6p21.3 2.24876 6.225758

375 222843_at FIGNL1 fidgetin-like 1 AK023411 Hs.137516.0 NP_071399 Chr:7p12.2 2.24831 3.306454375 222843_at FIGNL1 fidgetin-like 1 AK023411 Hs.137516.0 NP_071399 B: 7p12.2 2.24831 3.306454

376 221582_at HIST3H2A histone 3, H2a BC001193 gl 2654706 NP 254280 Chr.iq42.13 2.24661 1.702877376 221582_at HIST3H2A histone 3, H2a BC001193 gl 2654706 NP 254280 Chr.iq42.13 2.24661 1.702877

377 217983_s_at RNASET2 ribonuclease T2 /// ribonuclease NM_003730 g5231227 NP_003721 Chr:6q27 2.24551 6.991358 T2377 217983_s_at RNASET2 ribonuclease T2 /// ribonuclease NM_003730 g5231227 NP_003721 Chr: 6q27 2.24551 6.991358 T2

378 212200_at KIAA0692 KIAA0692 protein AK025933 Hs.100729.0 — Chr:12q24.33 2.24452 10.20301378 212200_at KIAA0692 KIAA0692 protein AK025933 Hs.100729.0 - Chr: 12q24.33 2.24452 10.20301

379 218115_at ASF1B ASF1 aπti-silencing function 1 NM_018154 g8922548 NP_060624 Chr:19p13.13 2.24271 1.578435 homolog B (S. cerevisiae)379 218115_at ASF1B ASF1 aπti-silencing function 1 NM_018154 g8922548 NP_060624 Chr: 19p13.13 2.24271 1.578435 homolog B (S. cerevisiae)

380 228955_at — Clone IMAGE-.4753714, mRNA AL041761 Hs.27379.0 — — 2.23903 3.62324 380 228955_at - Clone IMAGE-.4753714, mRNA AL041761 Hs.27379.0 - - 2.23903 3.62324

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

381 213226_at PMSCL1 polymyositis/scleroderma AI346350 Hs.91728.1 NP_005024 Chr:4q27 2.23729 4.640066 autoantigeπ 1 , 75kDa381 213226_at PMSCL1 polymyositis / scleroderma AI346350 Hs.91728.1 NP_005024 Chr: 4q27 2.23729 4.640066 autoantigeπ 1, 75kDa

382 203409_at DDB2 damage-specific DNA binding NM_000107 g4557514 NP_000098 Chr:11 p12-p11 2.23442 4.640066 protein 2, 48kDa /// damage- specific DNA binding protein 2, 48kDa382 203409_at DDB2 damage-specific DNA binding NM_000107 g4557514 NP_000098 Chr: 11 p12-p11 2.23442 4.640066 protein 2, 48kDa /// damage-specific DNA binding protein 2, 48kDa

383 221558_s_at LEF1 lymphoid enhancer-binding AF288571 g9858157 NP_057353 Chr:4q23-q25 2.22916 1.578435 factor 1 /// lymphoid enhancer- binding factor 1383 221558_s_at LEF1 lymphoid enhancer-binding AF288571 g9858157 NP_057353 Chr: 4q23-q25 2.22916 1.578435 factor 1 /// lymphoid enhancer-binding factor 1

384 218543_s_at ZC3HDC1 zinc finger CCCH type domain NM_022750 gl 2232412 NP_073587 Chr:7q34 2.22804 0.518167 containing 1384 218543_s_at ZC3HDC1 zinc finger CCCH type domain NM_022750 gl 2232412 NP_073587 Chr: 7q34 2.22804 0.518167 containing 1

385 202023 at EFNA1 ephrin-A1 /// ephrin-A1 NM 004428 g4758245 NP 004419 Chr:1q21-q22 2.22355 4.640066385 202023 at EFNA1 ephrin-A1 /// ephrin-A1 NM 004428 g4758245 NP 004419 Chr: 1q21-q22 2.22355 4.640066

386 226961_at LOC222171 hypothetical protein AI347918 Hs.91109.0 NP_787083 Chr:7p15.1 2.22251 13.5285 LOC222171Hypothetical protein AI347918 Hs.91109.0 NP_787083 Chr: 7p15.1 2.22251 13.5285 LOC222171

387 217925_s_at C6orf106 chromosome 6 open reading NM_022758 gl 2232426 NP_073595 Chr:6p21.31 2.22005 13.40329 frame 106387 217925_s_at C6orf106 chromosomes 6 open reading NM_022758 gl 2232426 NP_073595 Chr: 6p21.31 2.22005 13.40329 frame 106

388 224577 at KIAA1181 KIAA1181 protein AB033007 Hs.180428.1 NP 065195 Chr:5q35.2 2.21917 0.625663388 224577 at KIAA1181 KIAA1181 protein AB033007 Hs.180428.1 NP 065195 Chr: 5q35.2 2.21917 0.625663

389 240336_at — CDNA clone IMAGE:30337302, AI242749 Hs.146994.0 — — 2.21749 1.901012 S with apparent retained intron389 240336_at - CDNA clone IMAGE: 30337302, AI242749 Hs.146994.0 - - 2.21749 1.901012 S with apparent retained intron

390 218875_s_at FBXO5 F-box only protein 5 /// F-box NM_012177 g6912365 NPJD36309 Chr:6q25-q26 2.21632 11.06357 only proteiri 5390 218875_s_at FBXO5 F-box only protein 5 /// F-box NM_012177 g6912365 NPJD36309 Chr: 6q25-q26 2.21632 11.06357 only proteiri 5

391 209165_at AATF apoptosis antagonizing AF083208 g5813798 NP_036270 Chr:17q11.2- 2.21548 9.625715 transcription factor /// apoptosis q12 antagonizing transcription factor391 209165_at AATF apoptosis antagonizing AF083208 g5813798 NP_036270 Chr: 17q11.2- 2.21548 9.625715 transcription factor /// apoptosis q12 antagonizing transcription factor

392 221884 at EVH ecotropic viral integration Site 1 BE466525 Hs.234773.0 NP 005232 Chr:3q24-q28 2.21326 10.20301392 221884 at EVH ecotropic viral integration Site 1 BE466525 Hs.234773.0 NP 005232 Chr: 3q24-q28 2.21326 10.20301

393 238462_at AI418293 Hs.163714.0 2.21087 4.174189393 238462_at AI418293 Hs.163714.0 2.21087 4.174189

394 216194_s_at CKAP1 cytoskeleton associated protein AD001527 Hs.31053.1 NP_001272 Chr.19q13.11- 2.21032 9.625715 1 q13.12394 216194_s_at CKAP1 cytoskeleton associated protein AD001527 Hs.31053.1 NP_001272 Chr.19q13.11- 2.21032 9.625715 1 q13.12

395 208795_s_at MCM7 MCM7 minichromosome D55716 gl 255616 NP_005907 Chr:7q21.3- 2.20844 3.61384 maintenance deficient 7 (S. q22.1 cerevisiae)395 208795_s_at MCM7 MCM7 minichromosomes D55716 gl 255616 NP_005907 Chr: 7q21.3- 2.20844 3.61384 maintenance deficient 7 (p. Q22.1 cerevisiae)

396 219911_s_at SLCO4A1 soiute carrier organic anion NM_016354 g7706516 NP_057438 Chr:20q13.33 2.20818 4.174189 transporter family, member 4A1 /// soiute carrier organic anion transporter family, member 4A1 396 219911_s_at SLCO4A1 carrier organic anion NM_016354 g7706516 NP_057438 Chr: 20q13.33 2.20818 4.174189 transporter family, member 4A1 /// carrier organic anion transporter family, member 4A1

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

397 225687_at C20orf129 chromosome 20 open reading BCOO 1068 Hs.70704.0 NP_112181 Chr:20q11.22- 2.2016 2.814919 frame 129 q12397 225687_at C20orf129 chromosome 20 open reading BCOO 1068 Hs.70704.0 NP_112181 Chr: 20q11.22- 2.2016 2.814919 frame 129 q12

398 216270_at 7h3 hypothetical protein FLJ13511 AF209931 Hs.9291.1 NPJ49014 Chr:19p13.13 2.20112 2.814919398 216270_at 7h3 hypothetical protein FLJ13511 AF209931 Hs.9291.1 NPJ49014 Chr: 19p13.13 2.20112 2.814919

399 217871 s at MIF macrophage migration inhibitory NM_002415 g4505184 NP_002406 Chr:22q11.23 2.19656 4.149813 factor (glycosylation-inhibiting factor) /// macrophage migration inhibitory factor (glycosylation- inhibiting factor)399 217871 s at MIF macrophage migration inhibitory NM_002415 g4505184 NP_002406 Chr: 22q11.23 2.19656 4.149813 factor (glycosylation-inhibiting factor) /// macrophage migration inhibitory factor (glycosylation-inhibiting factor)

400 200837_at BCAP31 B-cell receptor-associated NM_005745 gl 0047078 NP. ,005736 Chr:xq28 2.19625 2.009521 protein 31 /// B-cell receptor- associated protein 31400 200837_at BCAP31 B-cell receptor-associated NM_005745 gl 0047078 NP. , 005736 Chr: xq28 2.19625 2.009521 protein 31 /// B-cell receptor-associated protein 31

401 33304_at ISG20 interferon stimulated gene U88964 4866504 NP. J302192 Chr:15q26 2.19586 4.174189 2OkDa401 33304_at ISG20 interferon stimulated genes U88964 4866504 NP. J302192 Chr: 15q26 2.19586 4.174189 2OkDa

402 201126 s at MGAT1 mannosyl (alpha-1,3-)- NM_002406 g6031182 NP_002397 Chr:5q35 2.19508 8.779357 glycoprotein beta-1 ,2-N- acetylglucosaminyltransferase /// mannosyl (alpha-1 ,3-)- glycoprotein beta-1, 2-N- o acetylglucosaminyltransferase402 201126 s at MGAT1 mannosyl (alpha-1,3 -) - NM_002406 g6031182 NP_002397 Chr: 5q35 2.19508 8.779357 glycoprotein beta-1,2-N-acetylglucosaminyltransferase /// mannosyl (alpha-1,3-glycoprotein beta-1 , 2-N-O-acetylglucosaminyltransferase

403 204146_at PIR51 RAD51-interacting protein BE966146 Hs.24596.0 NP_006470 Chr:12p13.2- 2.1934 4.149813 p13.1403 204146_at PIR51 RAD51-interacting protein BE966146 Hs.24596.0 NP_006470 Chr: 12p13.2- 2.1934 4.149813 p13.1

404 229099_at — Clone DNA64903 DSLR655 AW051379 Hs.9061.2 — — 2.19282 1.702877 (UNQ655) mRNA, complete cds404 229099_at - Clone DNA64903 DSLR655 AW051379 Hs. 9061.2 - - 2.19282 1.702877 (UNQ655) mRNA, complete cds

405 200996_at ACTR3 ARP3 actin-related protein 3 NM_005721 g7262289 NP_005712 Chr:2q14.1 2.19252 3.61384 homolog (yeast) /// ARP3 actin- related protein 3 homolog (yeast)405 200996_at ACTR3 ARP3 actin-related protein 3 NM_005721 g7262289 NP_005712 Chr: 2q14.1 2.19252 3.61384 homologue (yeast) /// ARP3 actin-related protein 3 homologue (yeast)

406 213485_s_at ABCC10 ATP-binding cassette, sub- AK000002 Hs.55879.1 NP_258261 Chr:6p21.1 2.19212 3.62324 family C (CFTR/MRP), member 10406 213485_s_at ABCC10 ATP-binding cassette, sub-AK000002 Hs.55879.1 NP_258261 Chr: 6p21.1 2.19212 3.62324 family C (CFTR / MRP), member 10

407 202188_at NUP93 nucleoporin 93kDa NM_014669 g7661901 NP_055484 Chr:16q13 2.19137 4.174189407 202188_at NUP93 nucleoporin 93kDa NM_014669 g7661901 NP_055484 Chr: 16q13 2.19137 4.174189

408 222077_s_at RACGAP1 Rac GTPase activating protein AU 153848 Hs.23900.1 NP_037409 Chr:12q13.12 2.18831 12.53374 1408 222077_s_at RACGAP1 Rac GTPase activating protein AU 153848 Hs.23900.1 NP_037409 Chr: 12q13.12 2.18831 12.53374 1

409 200949_x_at RPS20 ribosomal protein S20 NM_001023 g4506696 NP 001014 Chr:8q12 2.18386 3.61384409 200949_x_at RPS20 ribosomal protein S20 NM_001023 g4506696 NP 001014 Chr: 8q12 2.18386 3.61384

410 201521_s_at NCBP2 nuclear cap binding protein NM_007362 g6679063 NP_031388 Chr:3q29 2.18076 6.991358 subunit 2, 2OkDa 410 201521_s_at NCBP2 nuclear cap binding protein NM_007362 g6679063 NP_031388 Chr: 3q29 2.18076 6.991358 subunit 2, 2OkDa

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein IO Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein IO Localization Change (%)

411 200650_s_at LDHA lactate dehydrogenase A /// NM_005566 g5031856 NP_005557 Chr:11p15.4 2.17641 1.578435 lactate dehydrogenase A411 200650_s_at LDHA lactate dehydrogenase A /// NM_005566 g5031856 NP_005557 Chr: 11p15.4 2.17641 1.578435 lactate dehydrogenase A

412 225084_at SEC10L1 SEC10-like 1 (S. cerevisiae) BG 170743 Hs.29494.2 NP 006535 Chr:14q22.3 2.17373 13.5285412 225084_at SEC10L1 SEC10-like 1 (S. cerevisiae) BG 170743 Hs.29494.2 NP 006535 Chr: 14q22.3 2.17373 13.5285

413 215474_at — CDNA FLJ 12032 fis, clone AK022094 Hs.151194.0 — — 2.17232 4.174189 HEMBB1001880413 215474_at - CDNA FLJ 12032 fis, clone AK022094 Hs.151194.0 - - 2.17232 4.174189 HEMBB1001880

414 225562 at RASA3 RAS p21 protein activator 3 AI684746 Hs.119274.1 NP 031394 Chr:13q34 2.17137 11.55252414 225562 at RASA3 RAS p21 protein activator 3 AI684746 Hs.119274.1 NP 031394 Chr: 13q34 2.17137 11.55252

415 222701_s_at CHCHD7 coiled-coii-helix-coiled-coil-helix AA570393 Hs.323164.0 NPJ377276 Chr:8q11.23 2.17097 1.702877 domain containing 7415 222701_s_at CHCHD7 coiled-coii-helix-coiled-coil-helix AA570393 Hs.323164.0 NPJ377276 Chr: 8q11.23 2.17097 1.702877 domain containing 7

416 218788_s_at SMYD3 SET and MYND domain NM_022743 gl 2232400 NP_073580 Chr:1q44 2.16846 0.353669 containing 3416 218788_s_at SMYD3 SET and MYND domain NM_022743 gl 2232400 NP_073580 Chr: 1q44 2.16846 0.353669 containing 3

417 226121 at MGC23280 hypothetical protein MGC23280 AI076793 Hs.5163.0 NP 653284 Chr:17q11.2 2.16657 4.174189417 226121 at MGC23280 hypothetical protein MGC23280 AI076793 Hs.5163.0 NP 653284 Chr: 17q11.2 2.16657 4.174189

418 232946 s at Hs.307073.0 2.16498 0.625663418 232946 s at Hs.307073.0 2.16498 0.625663

419 214772_at G2 G2 protein H08993 Hs.30660.0 — Chr:11p13 2.16277 3.306454419 214772_at G2 G2 protein H08993 Hs.30660.0 - Chr: 11p13 2.16277 3.306454

420 207414_s_at PACE4 paired basic amino acid NM_002570 g4505576 NP_002561 Chr:15q26 2.15958 2.571322 cieaving System 4 /// paired basic amino acid cieaving system 4 W420 207414_s_at PACE4 paired basic amino acid NM_002570 g4505576 NP_002561 Chr: 15q26 2.15958 2.571322 cieaving system 4 /// paired basic amino acid cieaving system 4 W

421 205394_at CHEK1 CHK1 Checkpoint homolog (S. NM_001274 g4502802 NP_001265 Chr:11q24-q24 2.15766 6.225758 pombe)421 205394_at CHEK1 CHK1 checkpoint homologue (S. NM_001274 g4502802 NP_001265 Chr: 11q24-q24 2.15766 6.225758 pombe)

422 213695 at PON3 paraoxonase 3 L48516 Hs.296259.0 NP 000931 Chr:7q21.3 2.15709 14.22309422 213695 at PON3 paraoxonase 3 L48516 Hs.296259.0 NP 000931 Chr: 7q21.3 2.15709 14.22309

423 209408_at KIF2C kinesin family member 2C U63743 gl 695881 NP_006836 Chr:1p34.1 2.15608 8.153992423 209408_at KIF2C kinesin family member 2C U63743 gl 695881 NP_006836 Chr: 1p34.1 2.15608 8.153992

424 204318_s_at GTSE1 G-2 and S-phase expressed 1 NM_016426 g7705291 NP_057510 Chr:22q13.2- 2.15525 3.306454 q13.3424 204318_s_at GTSE1 G-2 and S-phase expressed 1 NM_016426 g7705291 NP_057510 Chr: 22q13.2- 2.15525 3.306454 q13.3

425 226594_at COQ6 coenzyme Q6 homolog (yeast) AA528157 Hs.285290.1 NP_057024 Chr:14q24.2 2.15491 3.306454425 226594_at COQ6 coenzyme Q6 homologue (yeast) AA528157 Hs.285290.1 NP_057024 Chr: 14q24.2 2.15491 3.306454

426 228037_at RARA retinoic acid receptor, alpha AA404273 Hs.12974.0 NP_000955 Chr:17q21 2.1548 9.840216426 228037_at RARA retinoic acid receptor, alpha AA404273 Hs.12974.0 NP_000955 Chr: 17q21 2.1548 9.840216

427 203559 s at ABP1 amiloride binding protein 1 NM_001091 g4501850 NP_001082 Chr:7q34-q36 2.15469 11.70204427 203559 s at ABP1 amiloride binding protein 1 NM_001091 g4501850 NP_001082 Chr: 7q34-q36 2.15469 11.70204

(amine oxidase (copper- containing)) /// amiioride binding protein 1 (amine oxidase (copper-containiπg))(amine oxidase (copper containing)) /// amiioride binding protein 1 (amine oxidase (copper containment))

428 218586. .at C20orf20 chromosome 20 open reading NM_018270 g8922763 NP. _060740 Chr:20q13.33 2.15349 4.640066 frame 20428 218586 . .at C20orf20 chromosomes 20 open reading NM_018270 g8922763 NP. _060740 Chr: 20q13.33 2.15349 4.640066 frame 20

429 214359. _s_at HSPCB heat shock 9OkDa protein 1 , A1218219 Hs.74335.1 NP. _031381 Chr:6p12 2.15194 8.779357 beta 429 214359. _s_at HSPCB heat shock 9OkDa protein 1, A1218219 Hs.74335.1 NP. _031381 Chr: 6p12 2.15194 8.779357 beta

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

430 205376_at INPP4B inositol polyphosphate-4- NM_003866 g4504706 NP_003857 Chr:4q31.1 2.14919 5.685182 phosphatase, type II, 105kDa /// inositol polyphosphate-4- phosphatase, type II, 105kDa430 205376_at INPP4B inositol polyphosphate-4-NM_003866 g4504706 NP_003857 Chr: 4q31.1 2.14919 5.685182 phosphatase, type II, 105kDa /// inositol polyphosphate-4-phosphatase, type II, 105kDa

431 224025_s_at APG7L APG7 autophagy 7-like (S. BC000091 gl 2652684 NP_006386 Chr:3p25.3- 2.14635 1.702877 cerevisiae) P25.2431 224025_s_at APG7L APG7 autophagy 7-like (S. BC000091 gl 2652684 NP_006386 Chr: 3p25.3- 2.14635 1.702877 cerevisiae) P25.2

432 225154_at SYAP1 Synapse associated protein 1 , BG029566 Hs.47334.0 NPJ 16185 Chr:xp22.22 2.14033 12.80219 SAP47 homolog (Drosophila)432 225154_at SYAP1 Synapse associated protein 1, BG029566 Hs.47334.0 NPJ 16185 Chr: xp22.22 2.14033 12.80219 SAP47 homolog (Drosophila)

433 217755_at HN1 hematological and neurological NM_016185 g7705876 NP_057269 Chr:17q25.2 2.13979 7.646445 expressed 1 /// hematological and neurological expressed 1433 217755_at HN1 hematological and neurological NM_016185 g7705876 NP_057269 Chr: 17q25.2 2.13979 7.646445 expressed 1 /// hematological and neurological expressed 1

434 219959_at MOCOS molybdenum cofactor sulfurase NM 017947 g8923660 NP_060417 Chr:18q12 2.13654 3.612633434 219959_at MOCOS molybdenum cofactor sulfurase NM 017947 g8923660 NP_060417 Chr: 18q12 2.13654 3.612633

435 236291_at RDH5 retinol dehydrogenase 5 (11-cis AI887702 Hs.222853.0 NP_002896 Chr:12q13-q14 2.13563 0.095261 and 9-cis)435 236291_at RDH5 retinol dehydrogenase 5 (11-cis AI887702 Hs.222853.0 NP_002896 Chr: 12q13-q14 2.13563 0.095261 and 9-cis)

436 226997_at — CDNA FLJ 10196 fis, clone W74476 Hs.12680.0 — 2.13549 1.223189 HEMBA1004776436 226997_at - CDNA FLJ 10196 fis, clone W74476 Hs.12680.0 - 2.13549 1.223189 HEMBA1004776

437 218681_s_at SDF2L1 stromal cell-derived factor 2-like NM_022044 gl 1545742 NP_071327 Chr:22q11.21 2.13478 3.612633 1 /// stromal cell-derived factor 2-like 1437 218681_s_at SDF2L1 stromal cell-derived factor 2-like NM_022044 gl 1545742 NP_071327 Chr: 22q11.21 2.13478 3.612633 1 /// stromal cell-derived factor 2-like 1

438 208734_x_at RAB2 RAB2, member RAS oncogene M28213 g550061 NP_002856 Chr:8q12.1 2.13437 4.149813 family438 208734_x_at RAB2 RAB2, member RAS oncogene M28213 g550061 NP_002856 Chr: 8q12.1 2.13437 4.149813 family

439 204328_at EVER1 epidermodysplasia NM__007267 g7305226 NP_009198 Chr:17q25.3 2.13283 2.489117 verruciformis 1 /// epidermodysplasia verruciformis 1439 204328_at EVER1 epidermodysplasia NM__007267 g7305226 NP_009198 Chr: 17q25.3 2.13283 2.489117 verruciformis 1 /// epidermodysplasia verruciformis 1

440 200697_at HK1 hexokinase 1 /// hexokinase 1 NM 000188 g4504390 NP 000179 Chr:10q22 2.13279 6.225758440 200697_at HK1 hexokinase 1 /// hexokinase 1 NM 000188 g4504390 NP 000179 Chr: 10q22 2.13279 6.225758

441 200822 x at TPH triosephosphate isomerase 1 NM 000365 g4507644 NP 000356 Chr:12p13 2.12957 1.578435441 200822 x at TPH triosephosphate isomerase 1 NM 000365 g4507644 NP 000356 Chr: 12p13 2.12957 1.578435

442 209739 s at DXS1283E GS2 gene AI814551 Hs.264.0 NP 004641 Chr:xp22.3 2.12857 3.612633442 209739 s at DXS1283E GS2 gene AI814551 Hs.264.0 NP 004641 Chr: xp22.3 2.12857 3.612633

443 219306_at KNSL7 kinesin-like 7 /// kinesin-like 7 NM_020242 g9910265 NP 064627 Chr:3p21.32 2.12819 3.612633443 219306_at KNSL7 kinesin-like 7 /// kinesin-like 7 NM_020242 g9910265 NP 064627 Chr: 3p21.32 2.12819 3.612633

444 201263_at TARS threonyl-tRNA synthetase /// NM_003191 g4507366 NP_003182 Chr:5p13.2 2.12564 9.625715 threonyl-tRNA synthetase444 201263_at TARS threonyl-tRNA synthetase /// NM_003191 g4507366 NP_003182 Chr: 5p13.2 2.12564 9.625715 threonyl-tRNA synthetase

445 214352_s_at KRAS2 v-Ki-ras2 Kirsten rat sarcoma 2 BF673699 Hs.184050.1 NP_004976 Chr:12p12.1 2.1234 8.153992 viral oncogene homolog445 214352_s_at KRAS2 v-Ki-ras2 Kirsten rat sarcoma 2 BF673699 Hs.184050.1 NP_004976 Chr: 12p12.1 2.1234 8.153992 viral oncogene homolog

446 201731_s_at TPR translocated promoter region (to NM_003292 g4507658 NP_003283 Chr:1q25 2.12301 2.814919 activated MET oncogene) 446 201731_s_at TPR translocated promoter region (to NM_003292 g4507658 NP_003283 Chr: 1q25 2.12301 2.814919 activated MET oncogene)

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

447 212568_s_at DLAT dihydrolipoamide S- BF978872 Hs.115285.1 NP_001922 Chr:11q23.1 2.12192 2.009521 acetyltransferase (E2 component of pyruvate dehydrogeπase complex)447 212568_s_at DLAT dihydrolipoamide S-BF978872 Hs.115285.1 NP_001922 Chr: 11q23.1 2.12192 2.009521 acetyltransferase (E2 component of pyruvate dehydrogeπase complex)

448 218252_at CKAP2 cytoskeleton associated protein NM_018204 g8922641 NP_060674 Chr:13q14 2.12172 1.578435 2448 218252_at CKAP2 cytoskeleton associated protein NM_018204 g8922641 NP_060674 Chr: 13q14 2.12172 1.578435 2

449 201463_s_at TALDO1 transaldolase 1 /// transaldolase NM_006755 g5803186 NP_006746 Chr:11p15.5- 2.11804 1.578435 1 p15.4449 201463_s_at TALDO1 transaldolase 1 /// transaldolase NM_006755 g5803186 NP_006746 Chr: 11p15.5- 2.11804 1.578435 1 p15.4

450 208918_s_at FLJ 13052 NAD kinase AI334128 Hs.220324.2 NP_075394 Chr:1p36.33- 2.11496 12.53374 p36.21450 208918_s_at FLJ 13052 NAD kinase AI334128 Hs.220324.2 NP_075394 Chr: 1p36.33- 2.11496 12.53374 p36.21

451 210951_x_at RAB27A RAB27A, member RAS AF125393 g5410356 NP_004571 Chr:15q15- 2.11397 6.991358 oncogene family q21.1451 210951_x_at RAB27A RAB27A, member RAS AF125393 g5410356 NP_004571 Chr: 15q15- 2.11397 6.991358 oncogene family q21.1

452 219402 s at MGC3067 hypothetical protein MGC3067 NM_024295 gl 3236515 NP_061100 Chr:8q24.13 2.11338 3.61384452 219402 s at MGC3067 hypothetical protein MGC3067 NM_024295 gl 3236515 NP_061100 Chr: 8q24.13 2.11338 3.61384

453 205241 _at SCO2 SCO cytochrome oxidase NM_005138 g4826991 NP_005129 Chr:22q13.33 2.11333 0.201325 deficient homolog 2 (yeast) /// SCO cytochrome oxidase deficient homolog 2 (yeast)453 205241 _at SCO2 SCO cytochrome oxidase NM_005138 g4826991 NP_005129 Chr: 22q13.33 2.11333 0.201325 deficient homologue 2 (yeast) /// SCO cytochrome oxidase deficient homologue 2 (yeast)

454 207717 s at PKP2 plakophilin 2 /// plakophilin 2 NM 004572 g4758931 NP 004563 Chr:12p11 2.10945 11.70204 w454 207717 s at PKP2 placophilin 2 /// plakophilin 2 NM 004572 g4758931 NP 004563 Chr: 12p11 2.10945 11.70204 w

455 224594 x at DCTN4 dynactin 4 (p62) AK025873 Hs.180952.1 NP 057305 Chr:5q31-q32 2.10916 9.266777455 224594 x at DCTN4 dynactin 4 (p62) AK025873 Hs.180952.1 NP 057305 Chr: 5q31-q32 2.10916 9.266777

456 233266 at — — AU159087 Hs.301408.0 — — 2.10912 4.174189456 233266 at - - AU159087 Hs301408.0 - - 2.10912 4.174189

457 201618_x_at GPAA1 GPAA1P anchor attachment NM_003801 g6031166 NP_003792 Chr:8q24.3 2.1071 5.685182 protein 1 homolog (yeast)457 201618_x_at GPAA1 GPAA1P anchor attachment NM_003801 g6031166 NP_003792 Chr: 8q24.3 2.1071 5.685182 protein 1 homolog (yeast)

458 231154_x_at TINAG tubulointerstitial nephritis BF000045 Hs.127011.1 NP_055279 Chr:6p11.2-p12 2.1052 9.266777 antigen458 231154_x_at TINAG tubulointerstitial nephritis BF000045 Hs.127011.1 NP_055279 Chr: 6p11.2-p12 2.1052 9.266777 antigen

459 221133 s at CLDN18 claudin 18 /// claudin 18 NM 016369 g7705960 NP 057453 Chr:3q22.3 2.10455 4.640066459 221133 s at CLDN18 claudin 18 /// claudin 18 NM 016369 g7705960 NP 057453 Chr: 3q22.3 2.10455 4.640066

460 200615_s_at AP2B1 adaptor-related protein complex AL567295 Hs.74626.0 NP_001273 Chr.17q11.2- 2.10448 3.612633 2, beta 1 subunit q12460 200615_s_at AP2B1 adapter-related protein complex AL567295 Hs.74626.0 NP_001273 Chr.17q11.2- 2.10448 3.612633 2, beta 1 subunit q12

461 205542__at STEAP six transmembrane epithelial NMJD 12449 g9558758 NP_036581 Chr:7q21 2.104 0.779487 antigen of the prostate /// six transmembrane epithelial antigen of the prostate461 205542__at STEAP six transmembrane epithelial NMJD 12449 g9558758 NP_036581 Chr: 7q21 2.104 0.779487 antigen of the prostate /// six transmembrane epithelial antigen of the prostate

462 217962_at NOLA3 nucleolar protein family A1 NM_018648 g8923941 NP_061118 Chr:15q14-q15 2.09683 9.625715 member 3 (H/ACA small nucleolar RNPs) 462 217962_at NOLA3 nucleolar protein family A 1 NM_018648 g8923941 NP_061118 Chr: 15q14-q15 2.09683 9.625715 member 3 (H / ACA small nucleolar RNPs)

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

463 205084_at BCAP29 B-celi receptor-associated NM_018844 g9994198 NP_061332 Chr:7q22-q31 2.09674 4.830969 protein 29 /// B-cell receptor- associated protein 29463 205084_at BCAP29 B-celi receptor-associated NM_018844 g9994198 NP_061332 Chr: 7q22-q31 2.09674 4.830969 protein 29 /// B-cell receptor-associated protein 29

464 205260_s_at ACYP1 acylphosphatase 1 , erythrocyte NM_001107 g4557244 NPJD01098 Chr:14q24.3 2.09558 4.640066 (common) type /// acylphosphatase 1 , erythrocyte (common) type464 205260_s_at ACYP1 acylphosphatase 1, erythrocyte NM_001107 g4557244 NPJD01098 Chr: 14q24.3 2.09558 4.640066 (common) type /// acylphosphatase 1, erythrocyte (common) type

465 200916_at TAGLN2 transgelin 2 NM_003564 g4507356 NP 003555 Chr:1q21-q25 2.09474 0.518167465 200916_at TAGLN2 transgelin 2 NM_003564 g4507356 NP 003555 Chr: 1q21-q25 2.09474 0.518167

466 219233_s_at GSDML gasdermin-like NM 018530 g8924175 NP 061000 Chr:17q21.2 2.09432 3.62324466 219233_s_at GSDML gasdermin-like NM 018530 g8924175 NP 061000 Chr: 17q21.2 2.09432 3.62324

467 219646_at FLJ20186 hypothetical protein FLJ20186 NM_017702 g8923176 NP 060172 Chr:16q24.3 2.08819 0.353669467 219646_at FLJ20186 hypothetical protein FLJ20186 NM_017702 g8923176 NP 060172 Chr: 16q24.3 2.08819 0.353669

468 232260_at ZBTB11 zinc finger and BTB domain T56840 Hs.301956.1 NP_055230 Chr:3q12.3 2.08724 2.814919 containing 11468 232260_at ZBTB11 zinc finger and BTB domain T56840 Hs.301956.1 NP_055230 Chr: 3q12.3 2.08724 2.814919 containing 11

469 218193 s at CGM 41 CGI-141 protein NM 016072 g7705635 NP 057156 Chr:12p12.3 2.08617 9.625715469 218193 s at CGM 41 CGI-141 protein NM 016072 g7705635 NP 057156 Chr: 12p12.3 2.08617 9.625715

470 202404_s_at COL1A2 collagen, type I, alpha 2 NM 000089 g4502946 NP 000080 Chr:7q22.1 2.08575 0.625663470 202404_s_at COL1A2 collagen, type I, alpha 2 NM 000089 g4502946 NP 000080 Chr: 7q22.1 2.08575 0.625663

471 206026_s_at TNFAIP6 tumor necrosis factor, alpha- NM_007115 g6005905 NPJ309046 Chr:2q24.1 2.08232 12.53374 induced protein 6 /// tumor necrosis factor, alpha-induced protein 6471 206026_s_at TNFAIP6 tumor necrosis factor, alpha NM_007115 g6005905 NPJ309046 Chr: 2q24.1 2.08232 12.53374 induced protein 6 /// tumor necrosis factor, alpha-induced protein 6

472 200658_s_at ZNF607 zinc finger protein 607 AL560017 Hs.75323.0 NP 116078 Chr:19q13.1 2.08161 6.225758472 200658_s_at ZNF607 zinc finger protein 607 AL560017 Hs.75323.0 NP 116078 Chr: 19q13.1 2.08161 6.225758

473 228846_at AW071793 Hs.109012.1 2.08118 1.380569473 228846_at AW071793 Hs.109012.1 2.08118 1.380569

474 220240_s_at C13orf11 chromosome 13 open reading NM_017905 g8923574 NP_060375 Chr:13q34 2.07978 8.779357 frame 11474 220240_s_at C13orf11 chromosome 13 open reading NM_017905 g8923574 NP_060375 Chr: 13q34 2.07978 8.779357 frame 11

475 213647_at DNA2L DNA2 DNA replication helicase D42046 Hs.194665.0 — Chr:10q21.3- 2.07889 11.70204 2-like (yeast) q22.1475 213647_at DNA2L DNA2 DNA replication helicase D42046 Hs.194665.0 - Chr: 10q21.3- 2.07889 11.70204 2-like (yeast) q22.1

476 209950 s at VILL villin-like /// villin-Iike BC004300 gl 3279166 NP 056957 Chr:3p21.3 2.07747 4.640066476 209950 s at VILL villin-like /// villin-iike BC004300 gl 3279166 NP 056957 Chr: 3p21.3 2.07747 4.640066

477 219010_at FLJ 10901 hypothetical protein FLJ10901 NM 018265 g8922753 NP 060735 Chr:1q32.1 2.07709 8.779357477 219010_at FLJ 10901 hypothetical protein FLJ10901 NM 018265 g8922753 NP 060735 Chr: 1q32.1 2.07709 8.779357

478 204779 s at HOXB7 homeo box B7 NM 004502 g4758551 NP 004493 Chr:17q21.3 2.07398 1.223189478 204779 s at HOXB7 homeo box B7 NM 004502 g4758551 NP 004493 Chr: 17q21.3 2.07398 1.223189

479 218399_s_at CDCA4 cell division cycle associated 4 NM_017955 g8923673 NP 060425 Chr:14q32.33 2.07163 12.53374479 218399_s_at CDCA4 cell division cycle associated 4 NM_017955 g8923673 NP 060425 Chr: 14q32.33 2.07163 12.53374

480 223035_s_at FARSLB phenylalanine-tRNA AF161521 g6841565 NP_005678 Chr.2q36.1- 2.0715 11.55252 synthetase-like, beta subunit q36.2480 223035_s_at FARSLB phenylalanine-tRNA AF161521 g6841565 NP_005678 Chr.2q36.1- 2.0715 11.55252 synthetase-like, beta subunit q36.2

481 218130_at MGC4368 hypothetical protein MGC4368 NM_024510 gl 3375647 NP_078786 Chr:17q25.3 2.07116 4.436744 481 218130_at MGC4368 hypothetical protein MGC4368 NM_024510 gl 3375647 NP_078786 Chr: 17q25.3 2.07116 4.436744

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

482 203560 at GGH gamma-glutamyl hydrolase NM 003878 g4503986 NP 003869 Chr:8q12.2 2.06852 1.380569482 203560 at GGH gamma-glutamyl hydrolase NM 003878 g4503986 NP 003869 Chr: 8q12.2 2.06852 1.380569

(conjugase, folylpoiygammaglutamyl hydrolase) /// gamma-glutamyl hydrolase (conjugase, folylpoiygammaglutamyl hydrolase)(conjugase, folylpoiygammaglutamyl hydrolase) /// gamma-glutamyl hydrolase (conjugase, folylpoiygammaglutamyl hydrolase)

483 206550_ _s_at NUP155 nucleoporin 155kDa /// NM_004298 g4758843 NP_ _004289 Chr:5p13.1 2.0655 8.153992 nucleoporin 155kDa483 206550_ _s_at NUP155 nucleoporin 155kDa /// NM_004298 g4758843 NP_ _004289 Chr: 5p13.1 2.0655 8.153992 nucleoporin 155kDa

484 208394_ x_a\ ESM 1 endothelial cell-specific NM_007036 gl 3259505 NP. .008967 Chr:5q11.2 2.06522 6.991358 molecule 1 /// endothelial cell- specific molecule 1484 208394_ x_a \ ESM 1 endothelial cell-specific NM_007036 gl 3259505 NP . , 008967 Chr: 5q11.2 2.06522 6.991358 molecule 1 /// endothelial cell-specific molecule 1

485 216088_ A_at PSMA7 proteasome (prosome, AL078633 Hs.233952.3 NP_ _002783 Chr;20q 13.33 2.06269 4.174189 macropain) subunit, alpha type, 7485 216088_ A_at PSMA7 proteasome (prosome, AL078633 Hs.233952.3 NP_ _002783 Chr; 20q 13.33 2.06269 4.174189 macropain) subunit, alpha type, 7

486 223029_ ^s_at RFWD1 ring finger and WD repeat AI806564 Hs.181077.0 NP. J 15647 Chr:16p13.3 2.06115 3.306454 domain 1486 223029_ ^ s_at RFWD1 ring finger and WD repeat AI806564 Hs.181077.0 NP. J 15647 Chr: 16p13.3 2.06115 3.306454 domain 1

487 201930 at MCM6 MCM6 minichromosome NM_005915 g7427518 NP_005906 Chr:2q21 2.06009 0.518167 maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae) /// MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae)487 201930 at MCM6 MCM6 minichromosomes NM_005915 g7427518 NP_005906 Chr: 2q21 2.06009 0.518167 maintenance deficient 6 (MIS5 homologous, S. pombe) (S. cerevisiae) /// MCM6 minichromosomes maintenance deficient 6 (MIS5 homologous, S. pombe) (S. cerevisiae )

488 45633 at FLJ 13912 hypothetical protein FLJ13912 AI421812 4858291_RC NP_073607 Chr:16q13 2.05674 14.22309488 45633 at FLJ 13912 hypothetical protein FLJ13912 AI421812 4858291_RC NP_073607 Chr: 16q13 2.05674 14.22309

489 201634 s at CYB5-M cytochrome b5 outer NM_030579 gl 3385593 NP_085056 Chr:16q22.1 2.05625 10.20301 mitochondrial membrane precursor /// cytochrome b5 outer mitochondrial membrane precursor489 201634 s at CYB5-M cytochrome b5 outer NM_030579 gl 3385593 NP_085056 Chr: 16q22.1 2.05625 10.20301 mitochondrial membrane precursor /// cytochrome b5 outer mitochondrial membrane precursor

490 204117 .at PREP prolyl endopeptidase NM. 002726 g4506042 NP 002717 Chr:6q22 2.05588 4.436744490 204117.at PREP prolyl endopeptidase NM . 002726 g4506042 NP 002717 BC: 6q22 2.05588 4.436744

491 201675_ _at AKAP1 A kinase (PRKA) anchor protein NM. _003488 g4502014 NP. _003479 Chr:17q21-q23 2.05464 1.901012 1 /// A kinase (PRKA) anchor protein 1491 201675_ _at AKAP1 A kinase (PRKA) anchor protein NM. _003488 g4502014 NP. _003479 Chr: 17q21-q23 2.05464 1.901012 1 /// A kinase (PRKA) anchor protein 1

492 218414_ _s_at NDE1 πudE nuclear distribution gene NM. J)17668 g8923109 NP. _060138 Chr:16p13.11 2.05248 3.306454 E homolog 1 (A. nidulans) 492 218414_ _s_at NDE1 πudE nuclear distribution gene NM . J) 17668 g8923109 NP . _060138 Chr: 16p13.11 2.05248 3.306454 E homolog 1 (A. nidulans)

Nr. Aflymetrix Geπsymbol Gβnname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Aflymetrix Symbol Gβnname Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

493 223839_s_at SCD stearoyl-CoA desaturase (delta- AF132203 gl 1493551 NPJ305054 Chr:10q23-q24 2.05218 4.640066 9-desaturase)493 223839_s_at SCD stearoyl-CoA desaturase (delta-AF132203 gl 1493551 NPJ305054 Chr: 10q23-q24 2.05218 4.640066 9-desaturase)

494 200041 _s_at BAT1 HLA-B associated transcript 1 NM_004640 g4758111 NP_004631 Chr:6p21.3 2.0514 3.62324 /// HLA-B associated transcript 1 /// HLA-B associated transcript 1494 200041 _s_at BAT1 HLA-B associated transcript 1 NM_004640 g4758111 NP_004631 Chr: 6p21.3 2.0514 3.62324 /// HLA-B associated transcript 1 /// HLA-B associated transcript 1

495 219588_s_at MTB more than blood homolog NM 017760 g8923292 NP 060230 Chr:7q36.3 2.05122 8.153992495 219588_s_at MTB more than blood homolog NM 017760 g8923292 NP 060230 Chr: 7q36.3 2.05122 8.153992

496 208816_x_at ANXA2P1 annexin A2 pseudogene 1 M62898 g187147 — Chr:4q21-q31 2.05011 3.612633496 208816_x_at ANXA2P1 annexin A2 pseudogenes 1 M62898 g187147 - Chr: 4q21-q31 2.05011 3.612633

497 222992_s_at NDUFB9 NADH dehydrogenase AF261090 g9802311 NP_004996 Chr:8q13.3 2.04865 12.80219 (ubiquinone) 1 beta subcomplex, 9, 22kDa497 222992_s_at NDUFB9 NADH dehydrogenase AF261090 g9802311 NP_004996 Chr: 8q13.3 2.04865 12.80219 (ubiquinone) 1 beta subcomplex, 9, 22kDa

498 218424 s at TSAP6 dudulin 2 NM 018234 g8922696 NP 060704 Chr:2q14.2 2.048 8.153992498 218424 s at TSAP6 dudulin 2 NM 018234 g8922696 NP 060704 Chr: 2q14.2 2048 8.153992

499 204070_at RARRES3 retinoic acid receptor responder NM_004585 g8051633 NP_004576 Chr:11q23 2.04735 5.685182 (tazarotene induced) 3 /// retinoic acid receptor responder (tazarotene induced) 3499 204070_at RARRES3 retinoic acid receptor responder NM_004585 g8051633 NP_004576 Chr: 11q23 2.04735 5.685182 (tazarotene induced) 3 /// retinoic acid receptor responder (tazarotene induced) 3

500 200750_s_at RAN RAN, member RAS oncogene AF054183 g4092053 NP_006316 Chr:12q24.3 2.04589 4.149813 family /// RAN, member RAS oncogene family500 200750_s_at RAN RAN, member RAS oncogene AF054183 g4092053 NP_006316 Chr: 12q24.3 2.04589 4.149813 family /// RAN, member RAS oncogene family

501 219544 at FLJ22624 FLJ22624 protein NM 024808 gl 3376190 NP 079084 Chr:13q21.33 2.04568 13.40329501 219544 at FLJ22624 FLJ22624 protein NM 024808 gl 3376190 NP 079084 Chr: 13q21.33 2.04568 13.40329

502 238617_at — CDNA FLJ38181 fis, clone AW207243 Hs.143134.0 — — 2.0449 4.174189 FCBBF1000125502 238617_at - CDNA FLJ38181 fis, clone AW207243 Hs.143134.0 - - 2.0449 4.174189 FCBBF1000125

503 221664_s_at F11R F11 receptor /// F11 receptor AF154005 g5457118 NP_058642 Chr.1q21.2- 2.04432 12.53374 q21.3503 221664_s_at F11R F11 receptor /// F11 receptor AF154005 g5457118 NP_058642 Chr.1q21.2- 2.04432 12.53374 q21.3

504 204126__s_at CDC45L CDC45 cell division cycle 45- NM_003504 g4502712 NPJD03495 Chr:22q11.21 2.0441 2.814919 like (S. cerevisiae) /// CDC45 cell division cycle 45-like (S. cerevisiae)504 204126__s_at CDC45L CDC45 cell division cycle 45- NM_003504 g4502712 NPJD03495 Chr: 22q11.21 2.0441 2.814919 like (S. cerevisiae) /// CDC45 cell division cycle 45-like (S. cerevisiae)

505 212116 at RFP ret finger protein NM 006510 Hs.142653.1 NP 006501 Chr:6p22 2.0439 5.685182505 212116 at RFP ret finger protein NM 006510 Hs.142653.1 NP 006501 Chr: 6p22 2.0439 5.685182

506 219787_s_at ECT2 epithelial cell transforming NMJ318098 g8922431 NPJ360568 Chr:3q26.1- 2.043 4.436744 sequeπce 2 oncogene q26.2506 219787_s_at ECT2 epithelial cell transforming NMJ318098 g8922431 NPJ360568 Chr: 3q26.1- 2.043 4.436744 sequeπce 2 oncogene q26.2

507 233380_s_at RUFY1 RUN and FYVE domain AA429145 Hs.306769.0 NP_079434 Chr:5q35.3 2.04084 2.08989 containing 1507 233380_s_at RUFY1 RUN and FYVE domain AA429145 Hs.306769.0 NP_079434 Chr: 5q35.3 2.04084 2.08989 containing 1

508 218856_at TNFRSF21 tumor necrosis factor receptor NM_016629 g7706171 NP_055267 Chr:6p21.1-12.2 2.04038 5.685182 superfamily, member 21 508 218856_at TNFRSF21 tumor necrosis factor receptor NM_016629 g7706171 NP_055267 Chr: 6p21.1-12.2 2.04038 5.685182 superfamily, member 21

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

509 217428_s_at COL10A1 Collagen, type X, alpha X98568 Hs.179729. 1 NPJD00484 Chr:6q21-q22 2.03775 13.40329 1(Schmid metaphyseal chondrodysplasia)509 217428_s_at COL10A1 Collagen, type X, alpha X98568 Hs.179729. 1 NPJD00484 Chr: 6q21-q22 2.03775 13.40329 1 (Schmid metaphyseal chondrodysplasia)

510 205601_s_at HOXB5 homeo box B5 /// homeo box NM_002147 g4504468 NP_002138 Chr:17q21.3 2.0364 2.489117 B5510 205601_s_at HOXB5 homeo box B5 /// homeo box NM_002147 g4504468 NP_002138 B: 17q21.3 2.0364 2.489117 B5

511 203557 s at PCBD 6-pyruvoyl-tetrahydropterin NM_000281 g4557830 NP_000272 Chr:10q22 2.03304 1.051298 synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) /// 6-pyruvoyl- tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1)511 203557 s at PCBD 6-pyruvyl-tetrahydropterin NM_000281 g4557830 NP_000272 Chr: 10q22 2.03304 1.051298 synthase / dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) /// 6-pyruvyl-tetrahydropterin synthase / dimerization cofactor of hepatocyte nuclear factor 1 alpha ( TCF1)

512 213681 at CYHR1 cysteine and histidine rieh 1 AW512817 Hs.173884.1 — Chr:8 2.03199 5.685182512 213681 at CYHR1 cysteine and histidine cattle 1 AW512817 Hs.173884.1 - Chr: 8 2.03199 5.685182

513 218186_at RAB25 RAB25, member RAS NM_020387 g9966860 NP_065120 Chr:1q22 2.03064 9.625715 oncogene family513 218186_at RAB25 RAB25, member RAS NM_020387 g9966860 NP_065120 Chr: 1q22 2.03064 9.625715 oncogene family

514 214431_at GMPS guanine monphosphate NM_003875 Hs.5398.0 NP_003866 Chr:3q24 2.03056 1.901012 synthetase /// guanine monphosphate synthetase514 214431_at GMPS guanine monphosphate NM_003875 Hs.5398.0 NP_003866 Chr: 3q24 2.03056 1.901012 synthetase /// guanine monophosphate synthetase

515 223082_at SH3KBP1 SH3-domain kinase binding AF230904 g7188748 NP_114098 Chr:xp22.1- 2.02671 13.5285 protein 1 p21.3515 223082_at SH3KBP1 SH3-domain kinase binding AF230904 g7188748 NP_114098 Chr: xp22.1- 2.02671 13.5285 protein 1 p21.3

516 215227 x at ACP1 aeid Phosphatase 1 , soluble BG035989 Hs.75393.2 NP 004291 Chr:2p25 2.02577 3.61384516 215227 x at ACP1 aeid phosphatase 1, soluble BG035989 Hs.75393.2 NP 004291 Chr: 2p25 2.02577 3.61384

517 201584_s_at DDX39 DEAD (Asp-Glu-Ala-Asp) box NM_005804 g5031658 NP_005795 Chr:19p13.13 2.02563 3.612633 Polypeptide 39 /// DEAD (Asp- Glu-Ala-Asp) box Polypeptide 39517 201584_s_at DDX39 DEAD (Asp-Glu-Ala-Asp) box NM_005804 g5031658 NP_005795 Chr: 19p13.13 2.02563 3.612633 Polypeptides 39 /// DEAD (Asp-Glu-Ala-Asp) box polypeptides 39

518 232602_at WFDC3 WAP four-disulfide core domain AL050348 Hs.149018.0 NP_542181 Chr:20q13.12 2.02116 1.901012 3518 232602_at WFDC3 WAP four-disulfide core domain AL050348 Hs.149018.0 NP_542181 Chr: 20q13.12 2.02116 1.901012 3

519 213789_at EBP emopamil binding protein (sterol N58493 Hs.75105.1 NP_006570 Chr:Xp11.23- 2.02051 3.306454 isomerase) P11.22519 213789_at EBP emopamil binding protein (sterol N58493 Hs.75105.1 NP_006570 Chr: Xp11.23-2.02051 3.306454 isomerase) P11.22

520 234372 at — — AL034403 Hs.247900.0 — — 2.02045 11.55252520 234372 at - - AL034403 Hs.247900.0 - - 2.02045 11.55252

521 201613 s at RUVBL1 RuvB-like 1 (E. coli) BC000519 gl 2653494 NP 003698 Chr:3q21 2.01994 0.353669521 201613 s at RUVBL1 RuvB-like 1 (E. coli) BC000519 gl 2653494 NP 003698 Chr: 3q21 2.01994 0.353669

522 200744_s_at GNB1 guanine nucleotide binding AI741124 Hs.215595.0 NPJD02065 Chr:1p36.33 2.01985 11.06357 protein (G protein), beta Polypeptide 1 522 200744_s_at GNB1 guanine nucleotide binding AI741124 Hs.215595.0 NPJD02065 Chr: 1p36.33 2.01985 11.06357 protein (G protein), beta Polypeptides 1

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

523 213892_s_at APRT adeπine AA927724 Hs.28914.1 NP_000476 Chr:16q24 2.0181 3.612633 phosphoribosyltransferase523 213892_s_at APRT adeπine AA927724 Hs.28914.1 NP_000476 Chr: 16q24 2.0181 3.612633 phosphoribosyltransferase

524 208689_s_at RPN2 ribophorin Il /// ribophorin Il BC003560 gl 3097707 NP_002942 Chr:20q12- 2.01751 6.991358 q13.1524 208689_s_at RPN2 ribophorin II /// ribophorin IL BC003560 gl 3097707 NP_002942 Chr: 20q12- 2.01751 6.991358 q13.1

525 212401_s_at CDC2L1 cell division cycle 2-like 1 AI767436 Hs.183418.19 NP_001778 Chr:1p36 2.01552 2.489117 (PITSLRE proteins)525 212401_s_at CDC2L1 cell division cycle 2-like 1 AI767436 Hs.183418.19 NP_001778 Chr: 1p36 2.01552 2.489117 (PITSLRE proteins)

526 210563_x_at CFLAR CASP8 and FADD-like U97075 g2253680 NPJ)03870 Chr:2q33-q34 2.01547 6.991358 apoptosis regυlator526 210563_x_at CFLAR CASP8 and FADD-like U97075 g2253680 NPJ) 03870 Chr: 2q33-q34 2.01547 6.991358 apoptosis regu- lator

527 220248_x_at NSFL1C NSFL1 (p97) cofactor (p47) /// NM_018839 gl 0047113 NP_057227 Chr:20p13 2.01267 10.20301 NSFL1 (p97) cofactor (p47)527 220248_x_at NSFL1C NSFL1 (p97) cofactor (p47) /// NM_018839 gl 0047113 NP_057227 Chr: 20p13 2.01267 10.20301 NSFL1 (p97) cofactor (p47)

528 200998_s_at CKAP4 cytoskeleton-associated protein AW029619 Hs.74368.0 NPJ306816 Chr:12q24.11 2.01238 8.153992 4528 200998_s_at CKAP4 cytoskeleton-associated protein AW029619 Hs.74368.0 NPJ306816 Chr: 12q24.11 2.01238 8.153992 4

529 212647_at RRAS related RAS viral (r-ras) NM_006270 Hs.9651.0 NP_006261 Chr:19q13.3- 2.01183 12.53374 oncogene homolog /// related qter RAS viral (r-ras) oncogene homolog529 212647_at RRAS related RAS viral (r-ras) NM_006270 Hs.9651.0 NP_006261 Chr: 19q13.3- 2.01183 12.53374 oncogene homologue /// related qter RAS viral (r-ras) oncogenes homolog

530 214316 x at MNT MAX binding protein AI378706 Hs.16488.3 NP 064706 Chr:17p13.3 2.0106 0.625663530 214316 x at MNT MAX binding protein AI378706 Hs.16488.3 NP 064706 Chr: 17p13.3 2.0106 0.625663

531 205807 s at TUFT1 tuftelin 1 /// tuftelin 1 NM_020127 g9910595 NP 064512 Chr:1q21 2.00955 3.306454531 205807 s at TUFT1 tuftelin 1 /// tuftelin 1 NM_020127 g9910595 NP 064512 BC: 1q21 2.00955 3.306454

532 222245 s at AF218012 Hs.72222.1 2.00634 6.225758532 222245 s at AF218012 Hs.72222.1 2.00634 6.225758

533 201590 x at ANXA2 annexin A2 /// annexin A2 NM 004039 g4757755 NP 004030 Chr:15q21-q22 2.00539 3.612633533 201590 x at ANXA2 annexin A2 /// annexin A2 NM 004039 g4757755 NP 004030 Chr: 15q21-q22 2.00539 3.612633

534 225300_at FLJ 14502 TRAF4 associated factor 1 BF792864 Hs.181466.0 — Chr:15q14 2.00517 1.702877534 225300_at FLJ 14502 TRAF4 associated factor 1 BF792864 Hs.181466.0 - Chr: 15q14 2.00517 1.702877

535 216942_s_at CD58 CD58 antigen, (iymphocyte D28586 Hs.75626.2 NPJ3O177O Chr:1p13 2.00461 3.306454 function-associated antigen 3) /// CD58 antigen, (Iymphocyte function-associated antigen 3)535 216942_s_at CD58 CD58 antigen, (iymphocyte D28586 Hs.75626.2 NPJ3O177O Chr: 1p13 2.00461 3.306454 function-associated antigen 3) /// CD58 antigen, (lymphocyte function-associated antigen 3)

536 213571_s_at EIF4EL3 eukaryotic translation initiation BF516289 Hs.19122.1 NP_004837 Chr:2q37.1 2.00426 7.646445 factor 4E-like 3536 213571_s_at EIF4EL3 eukaryotic translation initiation BF516289 Hs.19122.1 NP_004837 Chr: 2q37.1 2.00426 7.646445 factor 4E-like 3

537 201157_s_at NMT1 N-myristoyltransferase 1 /// N- AF020500 Hs.111039.0 NP_066565 Chr:17q21.31 2.00319 6.991358 myristoyltransferase 1537 201157_s_at NMT1 N-myristoyltransferase 1 /// N- AF020500 Hs.111039.0 NP_066565 Chr: 17q21.31 2.00319 6.991358 myristoyltransferase 1

538 221058 s at CKLF chemokine-like factor NM 016326 gl 0092611 NP_057410 Chr:16q22.1 2.00273 5.685182 538 221058 s at CKLF chemokine-like factor NM 016326 gl 0092611 NP_057410 Chr: 16q22.1 2.00273 5.685182

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung ChangeSample ID ID Protein ID Localization Change

539 212378 at GART phosphoribosylglycinamide NM 000819 Hs.82285.1 NP_000810 Chr:21q22.11 2.00253 6.991358 formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase /// phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase539 212378 at GART phosphoribosylglycinamides NM 000819 Hs.82285.1 NP_000810 Chr: 21q22.11 2.00253 6.991358 formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase /// phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase

540 200910 at CCT3 chaperonin containing TCP1 , NM 005998 g5174726 NP_005989 Chr.ϊq23 2.00023 1.578435 sυbunit 3 (gamma) /// chaperonin containing TCP1 , subunit 3 (gamma) 540 200910 at CCT3 chaperonin containing TCP1, NM 005998 g5174726 NP_005989 Chr.ϊq23 2.00023 1.578435 susbunit 3 (gamma) /// chaperonin containing TCP1, subunit 3 (gamma)

In Tabelle 2 sind Gene aufgeführt, deren Expression im duktalen Adenokarzinom des Pankreas im Mittel maximal halb so hoch ist wie die Expression in normalem Pankreasepithelgewebe. D. h. der Quotient („Fold Change") aus den Expressionswerten im mikrodissektierten duktalen Adenokarzinom des Pankreas (Mittel über 14 Patienten) und den Expressionswerten im gesunden mikrodissektierten duktalen Pankreasepithelgewebe (Mittel über 11 Personen) ist für diese Gene < 0,5. Wird für ein Gen der Quotient („Fold Change") 1000 angegeben, bedeutet dies, dass das Gen nur im Normalgewebe, aber nicht im Tumorgewebe nachgewiesen werden konnte. Die Gene sind ab der Nummer 541 fortlaufend nummeriert. Zur Charakterisierung werden jeweils die Identifikationsnummer des Affymetrix- Probensets, das Gensymbol, der Gen-Name, die chromosomale Lokalisation, die repräsentative Identifikationsnummer (ID), die Identifikationsnummer des Transkriptes und die des zugehörigen Proteins angegeben (Veröffentlichungsnummern in der Entrez- Datenbank des National Center for Biotechnology Information (NCBI), Bethesda, USA (http://www.ncbi.nih.gov/entrez/). Der q-Wert (in Prozent) gibt an, mit welchem statistischen Fehler das verwendete Auswerteprogramm eine differentielle Genexpression voraussagt. Table 2 lists genes whose expression in the ductal adenocarcinoma of the pancreas is on average at most half as high as the expression in normal pancreatic epithelial tissue. Ie. the fold change from the expression levels in the microdissected ductal adenocarcinoma of the pancreas (mean over 14 patients) and the expression values in the healthy microdissected ductal pancreas epithelium (mean over 11 persons) is <0.5 for these genes the quotient ("fold change") indicated 1000, this means that the gene could be detected only in normal tissue, but not in tumor tissue. The genes are consecutively numbered from the number 541. For characterization, the identification number of the Affymetrix sample set, the gene symbol, the gene name, the chromosomal localization, the representative identification number (ID), the identification number of the transcript and that of the corresponding protein are specified (publication numbers in the Entrez database of the National Center for Biotechnology Information (NCBI), Bethesda, USA (http://www.ncbi.nih.gov/entrez/) The q value (in percent) indicates the statistical error with which the evaluation program used predicts differential gene expression.

Tabelle 2Table 2

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung ChangeSample ID ID Protein ID Localization Change

541 235872 at Transcribed sequence BE408975 Hs.63335.0 1000 12.80219 with moderate simüarity to protein pdb:1 LBG (E. coli) B Chain B, Lactose Operon Repressor Bound To 21 -Base Pair Symmetrie Operator Dna, Alpha Carbons OnIy541 235872 at Transcribed sequence BE408975 Hs.63335.0 1000 12.80219 with moderate simunity to protein pdb: 1 LBG (E. coli) B chain B, lactose operon repressor Bound To 21 -Base Pair Symmetry Operator Dna, Alpha Carbons OnIy

542 235132_at LOC254128 hypothetical protein BF939830 Hs.260848.0 — Chr:2q14.2 1000 4.75532 LOC254128Hypothetical protein BF939830 Hs.260848.0 - Chr: 2q14.2 1000 4.75532 LOC254128

543 232014_at ZNF30 zinc finger protein 30 AI700188 Hs.107444.0 NP. _919306 Chr:19q13.13 1000 9.266777 (KOX 28)543 232014_at ZNF30 zinc finger protein 30 AI700188 Hs.107444.0 NP. _919306 Chr: 19q13.13 1000 9.266777 (KOX 28)

544 228323 at AF15Q14 AF15q 14 protein BF248364 Hs.283099.1 NP 065113 Chr:15q14 1000 9.266777544 228323 at AF15Q14 AF15q 14 protein BF248364 Hs.283099.1 NP 065113 Chr: 15q14 1000 9.266777

545 228095 at PHF14 PHD finger protein 14 AA608749 Hs.156276.1 — Chr:7p21.3 1000 6.023405545 228095 PHF14 PHD finger protein 14 AA608749 Hs.156276.1 - Chr: 7p21.3 1000 6.023405

546 214373_at Clone AI582773 Hs.125682.1 1000 3.612633 IMAGE-.5171739, mRNA546 214373_at Clone AI582773 Hs.125682.1 1000 3.612633 IMAGE-.5171739, mRNA

547 212552 at HPCAL1 hippocalcin-like 1 BE617588 Hs.3618.1 NP. 002140 Chr:2p25.1 1000 6.991358547 212552 at HPCAL1 hippocalcin-like 1 BE617588 Hs.3618.1 NP. 002140 Chr: 2p25.1 1000 6.991358

548 209773 s at RRM2 ribonucleotide BC001886 gl 2804874 NPJ)OI 025 Chr:2p25-p24 1000 3.612633 reduetase M2 Polypeptide /// ribonucleotide reduetase M2 Polypeptide548 209773 s at RRM2 ribonucleotide BC001886 gl 2804874 NPJ) OI 025 Chr: 2p25-p24 1000 3.612633 reduetase M2 polypeptides /// ribonucleotide reduetase M2 polypeptides

549 208741 _at SAP18 sin3-associated AW274856 Hs.23964.0 NP. _005861 Chr:13q12.11 1000 3.612633 Polypeptide, 18kDa549 208741 _at SAP18 sin3-associated AW274856 Hs.23964.0 NP. _005861 Chr: 13q12.11 1000 3,612,633 polypeptides, 18kDa

550 203177 _x_at TFAM transcription factor A, NM_003201 g4507400 NP. _003192 Chr:10q21 1000 6.991358 mitochondrial550 203177 _x_at TFAM transcription factor A, NM_003201 g4507400 NP. _003192 Chr: 10q21 1000 6.991358 mitochondrial

551 63009_ at FLJ10539 hypothetical protein A1188402 4860453JRC NP. _060600 Chr:3p14.2 1000 0.095261 FLJ 10539 551 63009_ at FLJ10539 hypothetical protein A1188402 4860453JRC NP. _060600 Chr: 3p14.2 1000 0.095261 FLJ 10539

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probenπummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

552 244573_at MRNA füll length insert R05505 Hs.193000.0 1000 0.095261 cDNA clone EUROIMAGE 200247552 244573_at MRNA filling length insert R05505 Hs.193000.0 1000 0.095261 cDNA clone EUROIMAGE 200247

553 243374_x_at — Transcribed sequeπces AW452674 Hs.202083.0 — — 1000 0.095261553 243374_x_at - Transcribed sequets AW452674 Hs.202083.0 - - 1000 0.095261

554 242330_at — Transcribed sequences AW195774 Hs.253199.0 — — 1000 0.149911554 242330_at - Transcribed sequences AW195774 Hs.253199.0 - - 1000 0.149911

555 241196 at — Transcribed sequences BE857611 Hs.157791.0 — — 1000 0.149911555 241196 at - Transcribed sequences BE857611 Hs.157791.0 - - 1000 0.149911

556 241192_at — Transcribed sequences BE327666 Hs.279637.0 — — 1000 0.095261556 241192_at - Transcribed sequences BE327666 Hs.279637.0 - - 1000 0.095261

557 240474_x_at — — AI631676 Hs.194984.0 — — 1000 0.518167557 240474_x_at - - AI631676 Hs.194984.0 - - 1000 0.518167

558 239241 at — Transcribed sequences AI015709 Hs.270552.0 — — 1000 2.08989558 239241 at - Transcribed sequences AI015709 Hs.270552.0 - - 1000 2.08989

559 234499_at Hs.306311.0 1000 0.095261559 234499_at Hs.306311.0 1000 0.095261

560 234341_x_at LOC91548 hypothetical protein AK026812 Hs.210844.0 — Chr:1q32.3 1000 0.095261 LOC91548560 234341_x_at LOC91548 hypothetical protein AK026812 Hs.210844.0 - Chr: 1q32.3 1000 0.095261 LOC91548

561 230114_at HOXB8 homeo box B8 AI492162 Hs.183274.1 NP 076921 Chr:17q21.3 1000 0.095261561 230114_at HOXB8 homeo box B8 AI492162 Hs.183274.1 NP 076921 Chr: 17q21.3 1000 0.095261

562 227951_s_at LOC147965 hypothetical protein AW338561 Hs.283656.0 NP_777565 Chr:19q13.2 1000 0.625663 LOC147965562 227951_s_at LOC147965 hypothetical protein AW338561 Hs.283656.0 NP_777565 Chr: 19q13.2 1000 0.625663 LOC147965

563 220809_at FLJ 14327 hypothetical protein NMJ024912 gl 3376375 — Chr:16q23.2 1000 0.095261 FLJ 14327563 220809_at FLJ 14327 hypothetical protein NMJ024912 gl 3376375 - Chr: 16q23.2 1000 0.095261 FLJ 14327

564 216749_at — CDNA: FLJ21198 fis, AK024851 Hs.306707.0 — — 1000 0.095261 clone COL00220564 216749_at - CDNA: FLJ21198 fis, AK024851 H.3306707.0 - - 1000 0.095261 clone COL00220

565 214569_at IFNA5 interferon, alpha 5 /// NM_002169 Hs.37113.0 NP_002160 Chr:9p22 1000 0.095261 Interferon, alpha 5565 214569_at IFNA5 interferon, alpha 5 /// NM_002169 Hs.37113.0 NP_002160 Chr: 9p22 1000 0.095261 Interferon, alpha 5

566 209959_at NR4A3 nuclear receptor U 12767 g924281 NP_008912 Chr:9q22 1000 0.095261 subfamily 4, group A, member 3566 209959_at NR4A3 nuclear receptor U 12767 g924281 NP_008912 Chr: 9q22 1000 0.095261 subfamily 4, group A, member 3

567 206913 at BAAT bile acid Coenzyme A: NM_001701 g4502350 NP_001692 Chr:9q22.3 1000 0.095261 amino acid N- acyltransferase (glycine N-choloyltransferase) /// bile acid Coenzyme A: amino acid N- acyltransferase (glycine N-choloyltransferase)567 206913 at BAAT bile acid coenzyme A: NM_001701 g4502350 NP_001692 Chr: 9q22.3 1000 0.095261 amino acid N-acyltransferase (glycine N-choloyltransferase) /// bile acid coenzyme A: amino acid N-acyltransferase (glycine N-choloyltransferase)

568 205563 at KISS1 KiSS-1 metastasis- NM_002256 g4504872 NPJ302247 Chr:1q32 1000 0.353669 suppressor/// KiSS-1 metastasis-suppressor 568 205563 at KISS1 KiSS-1 metastasis NM_002256 g4504872 NPJ302247 Chr: 1q32 1000 0.353669 suppressor /// KiSS-1 metastasis suppressor

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung ChangeSample ID ID Protein ID Localization Change

569 205290 s at BMP2 bone morphogeπetic NM_001200 g4557368 NP_0Q1191 Chr:20p12 1000 1.051298 protein 2569 205290 s at BMP2 bone morphogenetic NM_001200 g4557368 NP_0Q1191 Chr: 20p12 1000 1.051298 protein 2

570 206702 at TEK TEK tyrosine kinase, NM_000459 g4557868 NP_000450 Chr:9p21 0.49991 0.779487 endothelial (venous malformations, multiple cutaneoυs and mucosal) /// TEK tyrosine kinase, endothelial (venous malformations, multiple cutaneous and mucosal)570 206702 at TEK TEK tyrosine kinase, NM_000459 g4557868 NP_000450 Chr: 9p21 0.49991 0.779487 endothelial (venous malformations, multiple cutaneous and mucosal) /// TEK tyrosine kinase, endothelial (venous malformations, multiple cutaneous and mucosal)

571 232122. _s_ at Hs.126485.2 0.49987 0.201325571 232122. _s_ at Hs.126485.2 0.49987 0.201325

572 219503. _s_ at FLJ 11036 hypothetical protein NM_018306 g8922834 NP. _060776 Chr:3p25.2 0.49963 6.991358 FLJ11036572 219503. _s_ at FLJ 11036 hypothetical protein NM_018306 g8922834 NP. _060776 Chr: 3p25.2 0.49963 6.991358 FLJ11036

573 204271. _s_ at EDNRB endothelin receptor M74921 gl 82275 NP. _000106 Chr:13q22 0.49946 10.20301 type B /// endothelin receptor type B573 204271. _s_ at EDNRB endothelin receptor M74921 gl 82275 NP. _000106 Chr: 13q22 0.49946 10.20301 type B /// endothelin receptor type B

574 232392 at BE927772 Hs.167460.2 0.49943 0.353669574 232392 at BE927772 Hs.167460.2 0.49943 0.353669

575 223217 s at MAIL molecule possessing BE646573 Hs.301183.0 NP_113607 Chr:3p12-q12 0.49943 0.779487 ankyrin repeats induced by iipopolysaccharide (MAIL), homolog of mouse575 223217 s at MAIL molecule possessing BE646573 Hs.301183.0 NP_113607 Chr: 3p12-q12 0.49943 0.779487 ankyrin repeats induced by iipopolysaccharide (MAIL), homologue of mouse

576 219871. _at FLJ13197 hypothetical protein NM_024614 gl 3375829 NP. .078890 Chr:4p14 0.4994 0.201325 FLJ13197576 219871. _at FLJ13197 hypothetical protein NM_024614 gl 3375829 NP. , 078890 Chr: 4p14 0.4994 0.201325 FLJ13197

577 212423. jat Hs.28264.0 0.49853 1.223189577 212423. jat Hs.28264.0 0.49853 1.223189

578 204660. GFER growth factor, NMJD05262 ' g4885264 NP. .005253 Chr:16p13.3- 0.49836 0.625663 augmenter of liver p13.12 regeneration (ERV1 homolog, S. cerevisiae)578 204660. GFER growth factor, NMJD05262 ' g4885264 NP. .005253 Chr: 16p13.3- 0.49836 0.625663 augmenter of liver p13.12 regeneration (ERV1 homologous, S. cerevisiae)

579 221974. _at MRNA AW770748 Hs.5022.0 0.49835 0.518167579 221974. _at MRNA AW770748 Hs.5022.0 0.49835 0.518167

580 213547 at TIP120B TBP-interacting protein AB014567 Hs.154740.0 — Chr:3p25.2 0.49811 0.149911580 213547 at TIP120B TBP-interacting protein AB014567 Hs.154740.0 - Chr: 3p25.2 0.49811 0.149911

581 236225_ _at FLJ90165 hypothetical protein AI697028 Hs.130749.0 NP. _699169 Chr:17p13.3 0.49764 1.223189 FLJ90165 581 236225_ _at FLJ90165 hypothetical protein AI697028 Hs.130749.0 NP. _699169 Chr: 17p13.3 0.49764 1.223189 FLJ90165

Nr. Affymetrix Gensymbσl Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gensymbσl Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung ChangeSample ID ID Protein ID Localization Change

582 224379 at FTHL17 ferritin, heavy AF285592 gl 3603866 NP_114100 Chr:xp21 0.49679 0.095261 polypeptide-like 17 /// ferritin, heavy polypeptide-like 17582 224379 at FTHL17 ferritin, heavy AF285592 gl 3603866 NP_114100 Chr: xp21 0.49679 0.095261 polypeptide-like 17 /// ferritin, heavy polypeptide-like 17

583 205513 at TCN1 transcobalamin I NM_001062 g4507406 NP_001053 Chr:11q11-q12 0.49659 2.08989 (vitamin B12 binding protein, R binder family) /// transcobalamin I (vitamin B12 binding protein, R binder family)583 205513 at TCN1 transcobalamin I NM_001062 g4507406 NP_001053 Chr: 11q11-q12 0.49659 2.08989 (vitamin B12 binding protein, R binder family) /// transcobalamin I (vitamin B12 binding protein, R binder family)

584 228214 at Transcribed sequences AW242286 Hs.34145.0 0.49641 0.095261584 228214 at Transcribed sequences AW242286 Hs.34145.0 0.49641 0.095261

585 207643 s at TNFRSF1A tumor necrosis factor NM 001065 g4507574 NP_001056 Chr:12p13.2 0.49617 0.201325 receptor superfamily, member 1 A /// tumor necrosis factor receptor superfamily, member 1A585 207643 s at TNFRSF1A tumor necrosis factor NM 001065 g4507574 NP_001056 Chr: 12p13.2 0.49617 0.201325 receptor superfamily, member 1 A /// tumor necrosis factor receptor superfamily, member 1A

586 238868 at Transcribed sequence AW264269 Hs.98312.0 0.49572 0.201325 with weak similarity to protein ref:NP_060312.1 (H.sapiens) hypothetical protein FLJ20489 [Homo sapiens]586 238868 at Transcribed sequence AW264269 Hs.98312.0 0.49572 0.201325 with weak similarity to protein ref: NP_060312.1 (H.sapiens) hypothetical protein FLJ20489 [Homo sapiens]

587 209309_at AZGP1 alpha-2-glycoprotein 1 , D90427 g220150 NP_001176 Chr:7q22.1 0.49566 3.306454 zinc /// aIpha-2- glycoprotein 1 , zinc587 209309_at AZGP1 alpha-2-glycoprotein 1, D90427 g220150 NP_001176 Chr: 7q22.1 0.49566 3.306454 zinc /// aIpha-2 glycoprotein 1, zinc

588 213283_s_at SALL2 sal-like 2 (Drosophila) BG285616 Hs.79971.0 — Chr:14q11.1- 0.49493 0.149911 q12588 213283_s_at SALL2 sal-like 2 (Drosophila) BG285616 Hs.79971.0 - Chr: 14q11.1- 0.49493 0.149911 q12

589 227839_at MBD5 methyl-CpG binding AI911379 Hs.94125.1 NP_060798 Chr:2q23.3 0.49476 1.380569 domain protein 5589 227839_at MBD5 methyl-CpG binding AI911379 Hs.94125.1 NP_060798 Chr: 2q23.3 0.49476 1.380569 domain protein 5

590 229201 _at — CDNA FLJ42712 fis, AW044658 Hs.13326.0 — — _ 0.49466 2.489117 clone BRAMY3008044 590 229201 _at - CDNA FLJ42712 fis, AW044658 Hs.13326.0 - - _ 0.49466 2.489117 clone BRAMY3008044

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

591 230258 at ZNF515 zinc finger protein 515 AI277316 Hs.107924.0 NP 689842 Chr:9p24.2 0.49454 0.095261591 230258 at ZNF515 zinc finger protein 515 AI277316 Hs.107924.0 NP 689842 Chr: 9p24.2 0.49454 0.095261

592 205280 at GLRB glycine receptor, beta NM 000824 g4504022 NP 000815 Chr:4q31.3 0.49437 1.702877592 205280 at GLRB glycine receptor, beta NM 000824 g4504022 NP 000815 Chr: 4q31.3 0.49437 1.702877

593 225118_at SET8 PR/SET domain AI813938 Hs.111988.2 NP_065115 Chr:12q24.31 0.49415 0.095261 containing protein 8593 225118_at SET8 PR / SET domain AI813938 Hs.111988.2 NP_065115 Chr: 12q24.31 0.49415 0.095261 containing protein 8

594 217604 at Transcribed sequence AI086530 Hs.255883.0 0.49404 0.095261 with moderate similarity to protein sp:P39195594 217604 at Transcribed sequence AI086530 Hs.255883.0 0.49404 0.095261 with moderate similarity to protein sp: P39195

(H.sapiens)(H.sapiens)

ALU8_HUMAN Alu subfamily SX sequence contamination warning entryALU8_HUMAN Alu subfamily SX sequence contamination warning entry

595 219250 s at FLRT3 fibronectin leucine rieh NM_013281 g7019382 NP_037413 Chr:20p11 0.49373 3.612633 transmembrane protein 3 /// fibronectin leucine rieh transmembrane protein 3595 219250 s at FLRT3 fibronectin leucine rieh NM_013281 g7019382 NP_037413 Chr: 20p11 0.49373 3.612633 transmembrane protein 3 /// fibronectin leucine transmembrane protein 3

596 221112. _at IL1RAPL2 interleukin 1 receptor NM_017416 gl 1225606 NPJ359112 Chr:xq22.2- 0.49364 0.095261 accessory protein-like 2 q22.3 /// interleukin 1 receptor accessory protein-like 2596 221112 . _at IL1RAPL2 interleukin 1 receptor NM_017416 gl 1225606 NPJ359112 Chr: xq22.2- 0.49364 0.095261 protein-like protein 2 q22.3 /// interleukin 1 receptor accessory protein-like 2

597 233749_ _at CDNA clone AW139915 Hs.302098.0 — 0.49277 2.08989 IMAGE:30330955, partial eds597 233749_ _at CDNA clone AW139915 Hs.302098.0 - 0.49277 2.08989 IMAGE: 30330955, partial eds

598 216446, .at — CDNA: FLJ20915 fis, AK024568 Hs.306691.0 — — 0.4926 0.095261 clone ADSE00692598 216446 , .at - CDNA: FLJ20915 fis, AK024568 Hs.306691.0 - - 0.4926 0.095261 clone ADSE00692

599 226022_ .at SASH1 SAM and SH3 domain AU 144882 Hs.10299.0 NP_056093 Chr:6q24.3 0.492 0.779487 containing 1 599 226022_ .at SASH1 SAM and SH3 domain AU 144882 Hs.10299.0 NP_056093 Chr: 6q24.3 0.492 0.779487 containing 1

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript (D Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript (D Representative Chromosomal FoId q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

600 209617 s at CTNND2 cateπin (cadherin- AF035302 g2661061 NPJKM 323 Chr:5p15.2 0.49185 1.380569 associated protein), delta 2 (neural plakophilin-reiated arm- repeat protein) /// catenin (cadherin- associated protein), delta 2 (neural plakophilin-reiated arm- repeat protein)600 209617 s at CTNND2 cateπin (cadherin AF035302 g2661061 NPJKM 323 Chr: 5p15.2 0.49185 1.380569 associated protein), delta 2 (neural placophilin-reiated arm repeat protein) /// catenin (cadherin-associated protein), delta 2 ( neural placophilin-reiated arm-repeat protein)

601 234389 x at — — AF017336 Hs.248064.0 — — 0.49172 6.023405601 234389 x at - - AF017336 Hs.248064.0 - - 0.49172 6.023405

602 207072_at IL18RAP interleukin 18 receptor NM_003853 g4504656 NP_003844 Chr:2p24.3- 0.49117 8.153992 accessory protein /// p24.1 interleukin 18 receptor accessory protein602 207072_at IL18RAP interleukin 18 receptor NM_003853 g4504656 NP_003844 Chr: 2p24.3- 0.49117 8.153992 accessory protein /// p24.1 interleukin 18 receptor accessory protein

603 207720 at LOR loricrin /// loricrin NM 000427 g4557724 NP 000418 Chr.iq21 0.49076 0.095261603 207720 at LOR Loricrin /// loricrin NM 000427 g4557724 NP 000418 Chr.iq21 0.49076 0.095261

604 236600_at SPG20 spastic paraplegia 20, AI651603 Hs.46988.0 NP_055902 Chr:13q13.2 0.49066 0.095261 spartin (Troyer Syndrome)604 236600_at SPG20 spastic paraplegia 20, AI651603 Hs.46988.0 NP_055902 Chr: 13q13.2 0.49066 0.095261 spartin (Troyer Syndrome)

605 204042_at WASF3 WAS protein family, AB020707 Hs.82318.0 NPJ306637 Chr:13q12 0.49011 0.149911 member 3 /// WAS protein family, member 3605 204042_at WASF3 WAS protein family, AB020707 Hs.82318.0 NPJ306637 Chr: 13q12 0.49011 0.149911 member 3 /// WHAT protein family, member 3

606 206541_at KLKB1 kallikrein B, plasma NMJ)00892 g9257226 NP_000883 Chr:4q34-q35 0.48986 4.830969 (Fletcher factor) 1 /// kallikrein B, plasma (Fletcher factor) 1606 206541_at KLKB1 kallikrein B, plasma NMJ ) 00892 g9257226 NP_000883 Chr: 4q34-q35 0.48986 4.830969 (Fletcher factor) 1 /// kallikrein B, plasma (Fletcher factor) 1

607 206167_s_at ARHGAP6 Rho GTPase activating NMJ)O1174 g7382476 NP_001165 Chr:xp22.3 0.48983 0.353669 protein 6 /// Rho GTPase activating protein 6607 206167_s_at ARHGAP6 Rho GTPase activating NMJ) O1174 g7382476 NP_001165 Chr: xp22.3 0.48983 0.353669 protein 6 /// Rho GTPase activating protein 6

608 213939_s_at RIPX rap2 interacting protein AI871641 Hs.306623.0 NPJD55776 Chr:4q21.1 0.4895 1.380569608 213939_s_at RIPX rap2 interacting protein AI871641 Hs.306623.0 NPJD55776 Chr: 4q21.1 0.4895 1.380569

XX

609 234223_at — CDNA: FLJ21341 fis, AK024994 Hs .306738.0 — — 0.48853 0.201325 clone COL02653 609 234223_at - CDNA: FLJ21341 fis, AK024994 Hs. 306738.0 - - 0.48853 0.201325 clone COL02653

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

610 220341_s_at LOC51149 truncated calcium NM_016175 g7705864 NP_057259 Chr:5q35.3 0.48802 0.095261 binding protein610 220341_s_at LOC51149 truncated calcium NM_016175 g7705864 NP_057259 Chr: 5q35.3 0.48802 0.095261 binding protein

611 244069 at — — BE220584 Hs.88372.0 — — 0.48783 0.353669611 244069 at - - BE220584 Hs.88372.0 - - 0.48783 0.353669

612 232113 at Hs.50150.0 0.48775 2.08989612 232113 at Hs. 50150.0 0.48775 2.08989

613 225728_at — CDNA FU37284 fis, AI659533 Hs.278626.2 — 0.48747 0.095261 c!one BRAMY2013590613 225728_at - CDNA FU37284 fis, AI659533 Hs.278626.2 - 0.48747 0.095261 c! One BRAMY2013590

614 217273_at LOC343071 hypothetical protein AL022101 Hs.251419.0 — Chr:1p36.21 0.48742 0.095261 LOC343071Hypothetical protein AL022101 Hs.251419.0 - Chr: 1p36.21 0.48742 0.095261 LOC343071

615 242434_at — CDNA FLJ31093 fis, AW771952 Hs.191581.0 — 0.4872 0.625663 elone IMR321000161615 242434_at - CDNA FLJ31093 fis, AW771952 Hs.191581.0 - 0.4872 0.625663 elone IMR321000161

616 203752„s_at JUND jun D proto-oncogene NM_005354 gl 0938013 NPJD05345 Chr:19p13.2 0.48718 0.095261 /// jun D proto- oncogene616 203752 " s_at JUND jun D proto-oncogene NM_005354 gl 0938013 NPJD05345 Chr: 19p13.2 0.48718 0.095261 /// jun D protoncogene

617 203574_at NFIL3 nuclear factor, NM_005384 g4885516 NP_005375 Chr:9q22 0.48583 0.095261 interleukin 3 regulated /// nuclear factor, interleukin 3 regulated617 203574_at NFIL3 nuclear factor, NM_005384 g4885516 NP_005375 Chr: 9q22 0.48583 0.095261 interleukin 3 regulated /// nuclear factor, interleukin 3 regulated

618 216863_s_at ZCWCC1 zinc finger, CW-type AC004542 Hs.35276.1 Chr:22q12.2 0.48582 3.612633 with coiled-coil domain 1618 216863_s_at ZCWCC1 zinc finger, CW-type AC004542 Hs.35276.1 Chr: 22q12.2 0.48582 3.612633 with coiled-coil domain 1

619 227892_at CDNA clone AA855042 Hs.127301.0 0.48522 2.489117 IMAGE:5288757, partiai cds619 227892_at CDNA clone AA855042 Hs.127301.0 0.48522 2.489117 IMAGE: 5288757, partiai cds

620 224940__s_at PAPPA pregnancy-associated BF107618 Hs.250655.4 NP_002572 Chr:9q33.2 0.48521 2.814919 plasma protein A620 224940__s_at PAPPA pregnancy-associated BF107618 Hs.250655.4 NP_002572 Chr: 9q33.2 0.48521 2.814919 plasma protein A

621 228905 at Transcribed sequence BE672700 Hs.155272.0 -- 0.48519 0.149911 with weak similarity to protein sp:P39195 (H. sapiens) ALU8_HUMAN AIu sυbfamiiy SX sequence contamination warning entry621 228905 at Transcribed sequence BE672700 Hs.155272.0 - 0.48519 0.149911 with weak similarity to protein sp: P39195 (H. sapiens) ALU8_HUMAN AIu sυbfamiiy SX sequence contamination warning entry

622 219448, _at FLJ20533 hypothetical protein BC002748 gl 2803814 NP. _060336 Chr:8q13.3 0.48501 0.095261 FLJ20533622 219448 , _at FLJ20533 hypothetical protein BC002748 gl 2803814 NP. _060336 Chr: 8q13.3 0.48501 0.095261 FLJ20533

623 233000, _x_at DESR1 DESR1 protein AL035669 Hs.313498.0 NP. _996662 Chr:3p25.1 0.48433 1.380569 623 233000 , _x_at DESR1 DESR1 protein AL035669 Hs.313498.0 NP. _996662 Chr: 3p25.1 0.48433 1.380569

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

624 206959_s_at UPF3A UPF3 regulator of NM_023011 gl 2711675 NP_075387 Chr:13q34 0.48405 0.095261 nonsense transcripts homolog A (yeast)624 206959_s_at UPF3A UPF3 regulator of NM_023011 gl 2711675 NP_075387 Chr: 13q34 0.48405 0.095261 nonsense transcripts homologue A (yeast)

625 227174_at FLJ38736 hypothetical protein Z98443 Hs.86366.0 NP_877435 Chr:15q21.1 0.48385 1.380569 FLJ38736625 227174_at FLJ38736 hypothetical protein Z98443 Hs.86366.0 NP_877435 Chr: 15q21.1 0.48385 1.380569 FLJ38736

626 43427_at LOC283445 hypothetical protein AI970898 4811882_RC — Chr:12q24.12 0.48353 3.612633 LOC283445629 43427_at LOC283445 hypothetical protein AI970898 4811882_RC - Chr: 12q24.12 0.48353 3.612633 LOC283445

627 216483_s_at C19orf10 chromosome 19 open AC005339 Hs.296443.0 NP_061980 Chr:19p13.3 0.48326 0.201325 reading frame 10627 216483_s_at C19orf10 chromosomes 19 open AC005339 Hs.296443.0 NP_061980 Chr: 19p13.3 0.48326 0.201325 reading frame 10

628 227030_at — CDNA FU46579 fis, BG231773 Hs.318893.0 — — 0.48301 0.095261 clone THYMU3042758628 227030_at - CDNA FU46579 fis, BG231773 Hs.318893.0 - - 0.48301 0.095261 clone THYMU3042758

629 208437 at CLCN1 Chloride Channel 1 , NM_000083 g4502866 NPJ300074 Chr:7q35 0.48278 0.095261 skeletal muscle (Thomsen disease, autosomal dominant) /// Chloride Channel 1 , skeletal muscle (Thomsen disease, autosomal dominant)629 208437 at CLCN1 Chloride Channel 1, NM_000083 g4502866 NPJ300074 Chr: 7q35 0.48278 0.095261 skeletal muscle (Thomsen disease, autosomal dominant) /// Chloride Channel 1, skeletal muscle (Thomsen disease, autosomal dominant)

630 226344 _at KIAA1789 KIAA1789 protein A1741051 Hs.296317.0 — Chr:xq21 0.4818 0.518167630 226344 _at KIAA1789 KIAA1789 protein A1741051 Hs.296317.0 - Chr: xq21 0.4818 0.518167

631 222663 at RIOK2 RIO kinase 2 (yeast) AK001697 Hs.27021.0 NP 060813 Chr:5q15 0.48119 0.353669631 222663 at RIOK2 RIO kinase 2 (yeast) AK001697 Hs.27021.0 NP 060813 Chr: 5q15 0.48119 0.353669

632 214319_ _at W58342 Hs.181304.1 0.48088 3.612633632 214319_ _at W58342 Hs.181304.1 0.48088 3.612633

633 205259 at NR3C2 nuclear receptor NM_000901 g4505198 NP_000892 Chr:4q31.1 0.48084 9.625715 subfamily 3, group C, member 2 /// nuclear receptor subfamily 3, group C, member 2633 205259 at NR3C2 nuclear receptor NM_000901 g4505198 NP_000892 Chr: 4q31.1 0.48084 9.625715 subfamily 3, group C, member 2 /// nuclear receptor subfamily 3, group C, member 2

634 203973 s at CEBPD CCAAT/enhancer NM_005195 g4885130 NP_005186 Chr:8p11.2- 0.48026 0.095261 binding protein p11.1 (C/EBP), delta /// CCAAT/enhancer binding protein

Figure imgf000049_0001
634 203973 s at CEBPD CCAAT / enhancer NM_005195 g4885130 NP_005186 Chr: 8p11.2- 0.48026 0.095261 binding protein p11.1 (C / EBP), delta /// CCAAT / enhancer binding protein
Figure imgf000049_0001

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer JD Protein ID Lokalisierung ChangeSample Number JD Protein ID Localization Change

635 241368 at Transcribed sequence AH 90693 Hs.131034.0 0.47977 9.266777 with weak similarity to protein ref:NP_060312.1635 241368 at Transcribed sequence AH 90693 Hs.131034.0 0.47977 9.266777 with weak similarity to protein ref: NP_060312.1

(H.sapieπs) hypothetical protein(H.sapieπs) hypothetical protein

FLJ20489 [Homo sapiens]FLJ20489 [Homo sapiens]

636 216894_x_at CDKN 1 C cyclin-dependent D64137 Hs.106070.3 NP_000067 Chr.i 1p15.5 0.47941 2.814919 kinase inhibitor 1C (p57, Kip2)636 216894_x_at CDKN 1 C cyclin-dependent D64137 Hs.106070.3 NP_000067 Chr.i 1p15.5 0.47941 2.814919 kinase inhibitor 1C (p57, Kip2)

637 235424_at PIP5K1B phosphatidylinositol-4- N66727 Hs.10957.0 NP_003549 Chr:9q13 0.47937 0.149911 phosphate 5-kinase, type I, beta637 235424_at PIP5K1B phosphatidylinositol-4-N66727 Hs.10957.0 NP_003549 Chr: 9q13 0.47937 0.149911 phosphates 5-kinase, type I, beta

638 200732_s_at PTP4A1 protein tyrosine AL578310 Hs.227777.0 NP_003454 Chr:6q12 0.4782 0.095261 Phosphatase type IVA, member 1638 200732_s_at PTP4A1 protein tyrosine AL578310 Hs.227777.0 NP_003454 Chr: 6q12 0.4782 0.095261 Phosphatase type IVA, member 1

639 234557_at Sirnilar to hypothetical AK000806 Hs.306408.0 Chr:15q24.1 0.47815 0.095261 protein FLJ20452 (LOC390611 ), mRNA639 234557_at Sirnilar to hypothetical AK000806 Hs.306408.0 Chr: 15q24.1 0.47815 0.095261 protein FLJ20452 (LOC390611), mRNA

640 233155_at UPP2 uridine Phosphorylase AC005539 Hs.128427.1 NP_775491 Chr:2q24.2 0.47762 0.095261 2640 233155_at UPP2 uridine Phosphorylase AC005539 Hs.128427.1 NP_775491 Chr: 2q24.2 0.47762 0.095261 2

641 201427_s_at SEPP1 selenoprotein P, NM_005410 g4885590 NP_005401 Chr:5q31 0.47747 0.095261 plasma, 1 /// selenoprotein P, plasma, 1641 201427_s_at SEPP1 selenoprotein P, NM_005410 g4885590 NP_005401 Chr: 5q31 0.47747 0.095261 plasma, 1 /// selenoprotein P, plasma, 1

642 228063_s_at NAP1L5 nucleosome assembiy AW025330 Hs.12554.1 NP_715638 Chr:4q22.1 0.47741 0.149911 protein 1-Iike 5 642 228063_s_at NAP1L5 nucleosome assembiy AW025330 Hs.12554.1 NP_715638 B: 4q22.1 0.47741 0.149911 protein 1-Iike 5

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ITJ Protein ID Lokalisierung Change (%)Sample Number ITJ Protein ID Localization Change (%)

643 206008 at TGM1 transglutaminase 1 (K NM_000359 g4507474 NP_000350 Chr:14q11.2 0.47734 0.095261 Polypeptide epidermal type I, protein- glutamine-gamma- glutamyltransferase) /// transglutaminase 1 (K Polypeptide epidermal type I, protein- glutamine-gamma- glutamyltransferase)643 206008 at TGM1 transglutaminase 1 (K NM_000359 g4507474 NP_000350 Chr: 14q11.2 0.47734 0.095261 polypeptides epidermal type I, protein glutamine gamma glutamyltransferase) /// transglutaminase 1 (K polypeptides epidermal type I, protein glutamine gamma glutamyltransferase )

644 228782_at SCGB3A2 secretoglobin, family BG540454 Hs.54795.0 NP_473364 Chr:5q32 0.4772 0.518167 3A, member 2644 228782_at SCGB3A2 secretoglobin, family BG540454 Hs.54795.0 NP_473364 Chr: 5q32 0.4772 0.518167 3A, member 2

645 206417_at CNGA1 cyclic nucleotide gated NM_000087 g4502914 NP_000078 Chr:4p12-cen 0.47717 1.380569 Channel alpha 1 /// cyclic nucleotide gated Channel alpha 1645 206417_at CNGA1 cyclic nucleotide gated NM_000087 g4502914 NP_000078 Chr: 4p12-cen 0.47717 1.380569 Channel alpha 1 /// cyclic nucleotide gated Channel alpha 1

646 221099_at C9orf63 chromosome 9 open NM_017960 g8923683 — Chr:9q21.2 0.47706 0.095261 reading frame 63646 221099_at C9orf63 chromosomes 9 open NM_017960 g8923683 - Chr: 9q21.2 0.47706 0.095261 reading frame 63

647 244584_at — Transcribed sequences AW904533 Hs.101657.0 — — 0.47704 0.353669647 244584_at - Transcribed sequences AW904533 Hs.101657.0 - - 0.47704 0.353669

648 234986_at GCLM glutamate-cysteine AA630626 Hs.315562.0 NP_002052 Chr:1p22.1 0.47685 0.625663 ligase, modifier subunit648 234986_at GCLM glutamate cysteines AA630626 Hs.315562.0 NP_002052 Chr: 1p22.1 0.47685 0.625663 ligase, modifier subunit

649 230561_s_at FLJ23861 hypothetical protein AA252512 Hs.125845.1 NP_689732 Chr:2q34 0.47672 0.518167 FLJ23861649 230561_s_at FLJ23861 hypothetical protein AA252512 Hs.125845.1 NP_689732 Chr: 2q34 0.47672 0.518167 FLJ23861

650 228496_s_at CRIM1 cysteine-rich motor AW243081 Hs.19280.1 NP_057525 Chr:2p21 0.47622 0.095261 neuron 1650 228496_s_at CRIM1 cysteine-rich engine AW243081 Hs.19280.1 NP_057525 Chr: 2p21 0.47622 0.095261 neuron 1

651 218656_s_at LHFP lipoma HMGIC fusion NM_005780 g5031864 NP_005771 Chr:13q12 0.4762 0.625663 partner /// lipoma HMGIC fusion partner651 218656_s_at LHFP lipoma HMGIC fusion NM_005780 g5031864 NP_005771 Chr: 13q12 0.4762 0.625663 partner /// lipoma HMGIC fusion partner

652 212713_at MFAP4 microfibrillar-associated R72286 Hs.296049.0 NP_002395 Chr:17p11.2 0.47601 0.518167 protein 4652 212713_at MFAP4 microfibrillar-associated R72286 Hs.296049.0 NP_002395 Chr: 17p11.2 0.47601 0.518167 protein 4

653 229145_at C10orf104 chromosome 10 open AA541762 Hs.122586.0 NP_775744 Chr:10q22.2 0.47577 0.353669 reading frame 104653 229145_at C10orf104 chromosomes 10 open AA541762 Hs.122586.0 NP_775744 Chr: 10q22.2 0.47577 0.353669 reading frame 104

654 223366_at Clone BC004287 gl 3279127 0.47573 0.095261 IMAGE:3618365, mRNA 654 223366_at Clone BC004287 gl 3279127 0.47573 0.095261 IMAGE: 3618365, mRNA

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

655 231993_at — CDNA: FLJ23131 fis, AK026784 Hs.301296.1 — — 0.47494 1.380569 clone LNG08502655 231993_at - CDNA: FLJ23131 fis, AK026784 H.3301296.1 - - 0.47494 1.380569 clone LNG08502

656 234265_at LENG3 leukocyte receptor AF211968 Hs.306229.0 Chr:19q13.4 0.47458 1.380569 Cluster (LRC) member 3656 234265_at LENG3 leukocyte receptor AF211968 Hs.306229.0 Chr: 19q13.4 0.47458 1.380569 Cluster (LRC) member 3

657 206278_at PTAFR platelet-activating D10202 g219975 NPJ300943 Chr:1p35-p34.3 0.47421 0.095261 factor receptor /// platelet-activating factor receptor657 206278_at PTAFR platelet-activating D10202 g219975 NPJ300943 Chr: 1p35-p34.3 0.47421 0.095261 factor receptor /// platelet-activating factor receptor

658 217338 at LOC160313 keratiπ 19 pseudogene AB041269 Hs.272263.0 — Chr:12q22 0.47419 0.095261658 217338 at LOC160313 keratiπ 19 pseudogenic AB041269 Hs.272263.0 - Chr: 12q22 0.47419 0.095261

659 231944_at TM7SF1 transmembrane 7 AL045717 Hs.188228.0 NP_003263 Chr:1q42-q43 0.47395 1.051298 superfamily member 1 (upregulated in kidney)659 231944_at TM7SF1 transmembrane 7 AL045717 Hs.188228.0 NP_003263 Chr: 1q42-q43 0.47395 1.051298 superfamily member 1 (upregulated in kidney)

660 228806 at AI218580 Hs.81295.0 0.47391 0.353669660 228806 at AI218580 Hs.81295.0 0.47391 0.353669

661 211653 x at M33376 gl 87444 0.47386 1.223189661 211653 x at M33376 gl 87444 0.47386 1.223189

662 218901_at PLSCR4 phospholipid NM_020353 g9966818 NP_065086 Chr:3q24 0.47382 0.095261 scramblase 4 /// phospholipid Ul scramblase 4662 218901_at PLSCR4 phospholipid NM_020353 g9966818 NP_065086 Chr: 3q24 0.47382 0.095261 scramblase 4 /// phospholipid Ul scramblase 4

663 232640 at Hs.238928.2 0.47367 0.518167663 232640 at Hs.238928.2 0.47367 0.518167

664 215880_at NAGLU N- AI364950 Hs.50727.1 NP_000254 Chr:17q21 0.47322 0.149911 acetylglucosaminidase, alpha- (Sanfilippo disease HIB)664 215880_at NAGLU N-AI364950 Hs.50727.1 NP_000254 Chr: 17q21 0.47322 0.149911 acetylglucosaminidase, alpha (Sanfilippo disease HIB)

665 204135_at DOC1 downregulated in NM_014890 g7657036 NP_055705 Chr:3q12.3 0.47293 0.095261 ovarian Cancer 1 /// downregulated in ovarian Cancer 1665 204135_at DOC1 downregulated in NM_014890 g7657036 NP_055705 Chr: 3q12.3 0.47293 0.095261 ovarian cancer 1 /// downregulated in ovarian cancer 1

666 224000_at gl 2053292 0.47274 13.64212666 224000_at gl 2053292 0.47274 13.64212

667 218573_at MAGEH1 melanoma antigen, NM_014061 g7661529 NP_054780 Chr:xp11.22 0.47271 0.095261 family H, 1667 218573_at MAGEH1 melanoma antigen, NM_014061 g7661529 NP_054780 Chr: xp11.22 0.47271 0.095261 family H, 1

668 229738_at DNAH10 dynein, axonemal, AI700446 Hs.136245.0 — Chr:12q24.31 0.47242 2.814919 heavy Polypeptide 10668 229738_at DNAH10 dynein, axonemal, AI700446 Hs.136245.0 - Chr: 12q24.31 0.47242 2.814919 heavy polypeptides 10

669 229435 at ZNF515 zinc finger protein 515 AW025602 Hs.293854.0 NP 689842 Chr:9p24.2 0.47241 13.5285669 229435 at ZNF515 zinc finger protein 515 AW025602 Hs.293854.0 NP 689842 Chr: 9p24.2 0.47241 13.5285

670 56829_at T1 Tularik gene 1 H61826 4850333 RC NP 113654 Chr:8q24.3 0.47169 0.095261 670 56829_at T1 Tularik gene 1 H61826 4850333 RC NP 113654 Chr: 8q24.3 0.47169 0.095261

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

671 240414 at — Transcribed sequences AI963959 Hs.223825.0 — — 0.47161 0.095261671 240414 at - Transcribed sequences AI963959 Hs.223825.0 - - 0.47161 0.095261

672 232183_at SERAC1 serine active Site AA128978 Hs.154706.0 NP_116250 Chr:6q25.3 0.47092 0.353669 containing 1672 232183_at SERAC1 serine active Site AA128978 Hs.154706.0 NP_116250 Chr: 6q25.3 0.47092 0.353669 containing 1

673 227235_at — CDNA FLJ42250 fis, AI758408 Hs.22247.0 — — 0.47047 0.095261 clone TKIDN2007828673 227235_at - CDNA FLJ42250 fis, AI758408 Hs.22247.0 - - 0.47047 0.095261 clone TKIDN2007828

674 212845_at SAMD4 sterile alpha motif AB028976 Hs.173571.0 NP_056404 Chr:14q22.2 0.4704 0.095261 domain containing 4674 212845_at SAMD4 sterile alpha motif AB028976 Hs.173571.0 NP_056404 Chr: 14q22.2 0.4704 0.095261 domain containing 4

675 239262_at — CDNA FLJ26242 fis, AI498395 Hs.25930.0 — — 0.47039 4.174189 clone DMC00770675 239262_at - CDNA FLJ26242 fis, AI498395 Hs.25930.0 - - 0.47039 4.174189 clone DMC00770

676 236335_at Clone AW298375 Hs.24477.0 0.47004 12.80219 IMAGE-.5274212, mRNA676 236335_at Clone AW298375 Hs.24477.0 0.47004 12.80219 IMAGE-.5274212, mRNA

677 209550_at NDN necdin homolog U35139 gl 754970 NP_002478 Chr:15q11.2- 0.46981 0.518167 (mouse) /// necdin q12 homolog (mouse)677 209550_at NDN necdin homologue U35139 gl 754970 NP_002478 Chr: 15q11.2- 0.46981 0.518167 (mouse) /// necdin q12 homologue (mouse)

678 234319 at Hs.159200.1 0.46926 0.518167678 234319 at Hs.159200.1 0.46926 0.518167

679 59999_at H1F1AN hypoxia-inducible factor W37897 4852641 _RC NPJ360372 Chr:10q24 0.46903 12.53374 1 , alpha subunit inhibitor679 59999_at H1F1AN hypoxia-inducible factor W37897 4852641 _RC NPJ360372 Chr: 10q24 0.46903 12.53374 1, alpha subunit inhibitor

680 242033 at BE672684 Hs.109843.0 0.46874 1.380569680 242033 at BE672684 Hs.109843.0 0.46874 1.380569

681 238983_at FLJ14001 hypothetical protein AI538394 Hs.293514.0 NP_078953 Chr:4p14 0.4687 2.814919 FLJ14001681 238983_at FLJ14001 hypothetical protein AI538394 Hs.293514.0 NP_078953 Chr: 4p14 0.4687 2.814919 FLJ14001

682 206844_at FBP2 fructose-1,6- NM_003837 g4503670 NP_003828 Chr:9q22.3 0.46836 0.518167 bisphosphatase 2 /// fructose-1 ,6- bisphosphatase 2682 206844_at FBP2 fructose-1,6-NM_003837 g4503670 NP_003828 Chr: 9q22.3 0.46836 0.518167 bisphosphatase 2 /// fructose-1,6-bisphosphatase 2

683 216818_s_at OR2J2 olfactory receptor, AJ302559 Hs.307110.0 NPJ 12167 Chr:6p21.31- 0.46832 0.353669 family 2, subfamily J, 22.2 member 2683 216818_s_at OR2J2 olfactory receptor, AJ302559 Hs.307110.0 NPJ 12167 Chr: 6p21.31- 0.46832 0.353669 family 2, subfamily J, 22.2 member 2

684 202350 s at MATN2 matrilin 2 /// matrilin 2 NM 002380 gl 3518036 NP 002371 Chr:8q22 0.46826 3.612633684 202350 s at MATN2 matrilin 2 /// matrilin 2 NM 002380 gl 3518036 NP 002371 Chr: 8q22 0.46826 3.612633

685 204557_s_at DZIP1 DAZ interacting protein NM_014934 g7662435 NP_055749 Chr:13q32.1 0.46823 2.489117 1DZIP1 DAZ interacting protein NM_014934 g7662435 NP_055749 Chr: 13q32.1 0.46823 2.489117 1

686 232536 at Hs.222909.0 0.46812 0.095261686 232536 at Hs.222909.0 0.46812 0.095261

687 213832_at — Clone 24405 mRNA AA530995 Hs.23729.0 — - — 0.46801 0.095261 sequence687 213832_at - Clone 24405 mRNA AA530995 Hs.23729.0 - - - 0.46801 0.095261 sequence

688 225816_at PHF17 PHD finger protein 17 AW138134 Hs.12420.0 NPJD79176 Chr:4q26-q27 0.4676 0.095261 688 225816_at PHF17 PHD finger protein 17 AW138134 Hs.12420.0 NPJD79176 Chr: 4q26-q27 0.4676 0.095261

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

689 210990 s at LAMA4 iaminin, alpha 4 U77706 gl 684836 NP 002281 Chr:6q21 0.46718 0.353669689 210990 s at LAMA4 iaminine, alpha 4 U77706 gl 684836 NP 002281 Chr: 6q21 0.46718 0.353669

690 213800_at HF1 H factor 1 X04697 Hs.250651.2 NPJ)O0177 Chr:1q32 0.46717 0.095261 (compiement) /// H factor 1 (compiement)690 213800_at HF1 H factor 1 X04697 Hs.250651.2 NPJ) O0177 Chr: 1q32 0.46717 0.095261 (compiement) /// H factor 1 (compiement)

691 209305_s_at GADD45B growth arrest and DNA- AF078077 g3978391 NP_056490 Chr:19p13.3 0.46631 11.06357 damage-inducible, beta691 209305_s_at GADD45B growth arrest and DNA AF078077 g3978391 NP_056490 Chr: 19p13.3 0.46631 11.06357 damage-inducible, beta

692 226841 _at MPEG1 macrophage expressed BF590697 Hs.288581.0 — Chr:11q12.2 0.46621 0.518167 gene 1692 226841 _at MPEG1 macrophage expressed BF590697 Hs.288581.0 - Chr: 11q12.2 0.46621 0.518167 gene 1

693 213771_at IRF2BP1 interferon regulatory A1401612 Hs.154680.0 NP_056464 Chr:19q13.32 0.46595 0.095261 factor 2 biπding protein 1693 213771_at IRF2BP1 interferon regulatory A1401612 Hs.154680.0 NP_056464 Chr: 19q13.32 0.46595 0.095261 factor 2 biπding protein 1

694 205074 at SLC22A5 solute carrier family 22 NM_003060 g4507004 NP_003051 Chr:5q31 0.46568 0.095261694 205074 at SLC22A5 solute carrier family 22 NM_003060 g4507004 NP_003051 Chr: 5q31 0.46568 0.095261

(organic cation transporter), member 5 /// solute carrier family 22 (organic cation 'Jl transporter), member 5(organic cation transporter), member 5 /// solute carrier family 22 (organic cation 'Jl transporter), member 5

695 205857_at SLC18A2 solute carrier family 18 AI269290 Hs.1813.0 NP_003045 Chr:10q25 0.46561 3.62324 (vesicular monoamine), member 2695 205857_at SLC18A2 solute carrier family 18 AI269290 Hs.1813.0 NP_003045 Chr: 10q25 0.46561 3.62324 (vesicular monoamine), member 2

696 216535 at Hs.274514.0 0.46554 0.095261696 216535 at Hs.274514.0 0.46554 0.095261

697 210328_at GNMT glycine N- AF101477 g8671583 NP_061833 Chr.βp12 0.46521 4.436744 methyltransferase /// glycine N- methyitransferase697 210328_at GNMT glycine N-AF101477 g8671583 NP_061833 Chr. Β p12 0.46521 4.436744 methyltransferase /// glycine N-methyltransferase

698 228229 at ZNF526 zinc finger protein 526 BF512165 Hs.137282.0 NP 597701 Chr:19q13.31 0.46518 0.095261Zinc finger protein 526 BF512165 Hs.137282.0 NP 597701 Chr: 19q13.31 0.46518 0.095261

699 240771_at MGC33370 hypothetical protein AH 39906 Hs.104926.0 NP_776168 Chr:1q44 0.46459 3.306454 MGC33370699 240771_at MGC33370 hypothetical protein AH 39906 Hs.104926.0 NP_776168 Chr: 1q44 0.46459 3.306454 MGC33370

700 201694_s_at EGR1 early growth response NM_001964 g4503492 NP_001955 Chr:5q31.1 0.46322 0.095261 1 /// early growth response 1700 201694_s_at EGR1 early growth response NM_001964 g4503492 NP_001955 BC: 5q31.1 0.46322 0.095261 1 /// early growth response 1

701 203419_at MLL4 myeloid/iymphoid or NMJD14727 g7662045 NP_055542 Chr:19q13.1 0.46311 0.149911 mtxed-lineage leukemia 4701 203419_at MLL4 myeloid / iymphoid or NMJD14727 g7662045 NP_055542 Chr: 19q13.1 0.46311 0.149911 mtxed-lineage leukemia 4

702 217154_s_at EDN3 endothelin 3 AL035250 Hs.1408.2 NP_000105 Chr:20q13.2- 0.46217 0.095261 q13.3 702 217154_s_at EDN3 endothelin 3 AL035250 Hs.1408.2 NP_000105 Chr: 20q13.2- 0.46217 0.095261 q13.3

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

703 210158 at ERCC4 excision repair cross- U64315 gl 524410 NP_005227 Chr:16p13.3- 0.46214 0.149911 complementing rodent p13.11 repair deficiency, complemeπtation group 4 /// excision repair cross-complementing rodent repair deficiency, complementation group 4703 210158 at ERCC4 excision repair cross- U64315 gl 524410 NP_005227 Chr: 16p13.3- 0.46214 0.149911 complementing rodent p13.11 repair deficiency, compliance group 4 /// excision repair cross-complementing rodent repair deficiency, complementation group 4

704 228943 at MAP6 microtubule-associated AW003666 Hs.239444.0 Chr:11q13.3 0.46211 0.095261 protein 6704 228943 at MAP6 microtubule-associated AW003666 Hs.239444.0 Chr: 11q13.3 0.46211 0.095261 protein 6

705 221366 at NKX6-1 NK6 transcription factor NM_006168 g5453787 NP_006159 Chr:4q21.2-q22 0.46195 0.201325 related, locus 1 (Drosophila) /// NK6 transcription factor related, locus 1 (Drosophila)705 221366 at NKX6-1 NK6 transcription factor NM_006168 g5453787 NP_006159 Chr: 4q21.2-q22 0.46195 0.201325 related, locus 1 (Drosophila) /// NK6 transcription factor related, locus 1 (Drosophila)

706 207281. _x_at VCX2 variable Charge, X- NM_ _016378 g7706758 NP_ _057462 Chr:xp22.32 0.46162 0.518167 linked 2 /// variable Charge, X-linked 2706 207281. _x_at VCX2 variable batch, X- NM_ _016378 g7706758 NP_ _057462 Chr: xp22.32 0.46162 0.518167 linked 2 /// variable batch, X-linked 2

707 225946 at Hs.44787.0 0.46111 0.095261707 225946 at Hs.44787.0 0.46111 0.095261

708 206197 at NME5 non-metastatic cells 5, NM_003551 g4505412 NP_003542 Chr:5q31 0.46104 0.095261 protein expressed in (nucleoside- diphosphate kinase) /// non-metastatic cells 5, protein expressed in (nucleoside- diphosphate kinase)708 206197 at NME5 non-metastatic cells 5, NM_003551 g4505412 NP_003542 Chr: 5q31 0.46104 0.095261 protein expressed in (nucleoside diphosphate kinase) /// non-metastatic cells 5, protein expressed in (nucleoside diphosphate kinase)

709 239799. _at — CDNA FLJ34826 fis, BF592774 Hs.179153.0 — — 0.46074 0.095261 clone NT2NE2008803709 239799. _at - CDNA FLJ34826 fis, BF592774 Hs.179153.0 - - 0.46074 0.095261 clone NT2NE2008803

710 203197. _s_at FLJ20580 hypothetical protein AW157077 Hs.146861.0 NP. ,060357 Chr:1p32.3 0.46023 12.53374 FLJ20580710 203197. _s_at FLJ20580 hypothetical protein AW157077 Hs.146861.0 NP. , 060357 Chr: 1p32.3 0.46023 12.53374 FLJ20580

711 212279. _at MAC30 hypothetical protein BE779865 Hs.199695.0 NP. ,055388 Chr:17q11.2 0.46015 0.625663 MAC30 711 212279. _at MAC30 hypothetical protein BE779865 Hs.199695.0 NP. , 055388 Chr: 17q11.2 0.46015 0.625663 MAC30

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

712 226682 at AW006185 Hs.293968.0 0.46006 0.095261712 226682 at AW006185 Hs.293968.0 0.46006 0.095261

713 202388_at RGS2 regulator of G-protein NM_002923 g4506516 NP_002914 Chr:1q31 0.45998 0.095261 signalling 2, 24kDa /// regulator of G-protein signalling 2, 24kDa713 202388_at RGS2 regulator of G-protein NM_002923 g4506516 NP_002914 Chr: 1q31 0.45998 0.095261 signaling 2, 24kDa /// regulatory of G-protein signaling 2, 24kDa

714 228469_at PPID peptidylprolyl BF431902 Hs.143482.1 NP_005029 Chr:4q31.3 0.45996 0.149911 isomerase D (cyclophilin D)714 228469_at PPID peptidylprolyl BF431902 Hs.143482.1 NP_005029 Chr: 4q31.3 0.45996 0.149911 isomerase D (cyclophilin D)

715 229449_at — CDNA FLJ36553 fis, BE348404 Hs.24740.0 — — 0.45986 0.149911 clone TRACH2008478715 229449_at - CDNA FLJ36553 fis, BE348404 Hs.24740.0 - - 0.45986 0.149911 clone TRACH2008478

716 231460 at Transcribed sequence AI702438 Hs.145533.0 — 0.45981 0.149911 with moderate similarity to protein pdb:1LBG (E. coli) B Chain B,716 231460 at Transcribed sequence AI702438 Hs.145533.0 - 0.45981 0.149911 with moderate similarity to protein pdb: 1LBG (E. coli) B chain B,

Lactose OperonLactose operon

Repressor Bound ToRepressor Bound To

21-Base Pair21-base pair

Symmetrie Operator Ul UlSymmetry operator Ul Ul

Dna, Alpha CarbonsDNA, Alpha Carbons

OnIyOnIy

717 233263_at — CDNA FLJ12236 fis, AU147635 Hs.296708.0 — — 0.4597 1.901012 clone MAMMA1001244717 233263_at - CDNA FLJ12236 fis, AU147635 Hs.296708.0 - - 0.4597 1.901012 clone MAMMA1001244

718 225662_at 2AK sterile alpha motif and H28667 Hs.16930.0 NP„057737 Chr:2q24.2 0.45955 1.380569 leucine zipper containing kinase AZK718 225662_at 2AK sterile alpha motif and H28667 Hs.16930.0 NP " 057737 Chr: 2q24.2 0.45955 1.380569 leucine zipper containing kinase AZK

719 225975 at PCDH18 protocadherin 18 AW189885 Hs.97266.0 NP 061908 Chr:4q31 0.4595 0.095261719 225975 at PCDH18 protocadherin 18 AW189885 Hs.97266.0 NP 061908 Chr: 4q31 0.4595 0.095261

720 204814_at CADPS Ca2+-dependent NM_003716 g4502540 NP_003707 Chr:3p21.1 0.45933 7.646445 secretion activator /// Ca2+-dependent secretion activator720 204814_at CADPS Ca2 + -dependents NM_003716 g4502540 NP_003707 Chr: 3p21.1 0.45933 7.646445 secretion activator /// Ca2 + -dependent secretion activator

721 229623_at CDNA clone BF508344 Hs.112742.0 — Chr:4q21.3 0.45925 1.702877 IMAGE:6301163, containing frame-shift errors 721 229623_at CDNA clone BF508344 Hs.112742.0 - Chr: 4q21.3 0.45925 1.702877 IMAGE: 6301163, containing frame-shift errors

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

722 205203_at PLD1 phospholipase DI, NM_002662 g4505872 NPJ302653 Chr:3q26 0.45919 4.149813 phophatidylcholine- specific722 205203_at PLD1 phospholipase DI, NM_002662 g4505872 NPJ302653 Chr: 3q26 0.45919 4.149813 phophatidylcholine-specific

723 207542_s_at AQP1 aquaporin 1 (channel- NM_000385 g4755121 NP_000376 Chr:7p14 0.45902 0.095261 forming integral protein, 28kDa)723 207542_s_at AQP1 aquaporin 1 (channel- NM_000385 g4755121 NP_000376 Chr: 7p14 0.45902 0.095261 forming integral protein, 28kDa)

724 240043 at — Transcribed sequences A1130988 Hs.130370.0 — — 0.45865 0.095261724 240043 at - Transcribed sequences A1130988 Hs.130370.0 - - 0.45865 0.095261

725 228974_at MGC48625 hypothetical protein AI816281 Hs.293690.0 NP_872415 Chr:19q 13.42 0.45843 0.353669 MGC48625725 228974_at MGC48625 hypothetical protein AI816281 Hs.293690.0 NP_872415 Chr: 19q 13.42 0.45843 0.353669 MGC48625

726 210294_at TAPBP TAP binding protein AF067286 gl 0803729 NP_003181 Chr:6p21.3 0.45809 0.095261 (tapasin)726 210294_at TAPBP TAP binding protein AF067286 gl 0803729 NP_003181 Chr: 6p21.3 0.45809 0.095261 (tapasin)

727 230403_at — CDNA FLJ45905 fis, AV723984 Hs.7154.0 — 0.45743 0.779487 clone OCBBF3026576727 230403_at - CDNA FLJ45905 fis, AV723984 Hs.7154.0 - 0.45743 0.779487 clone OCBBF3026576

728 227705_at MGC23947 hypothetical protein BF591534 Hs.21861.0 NP_689491 Chr:xq22.1 0.45633 1.051298 MGC23947728 227705_at MGC23947 hypothetical protein BF591534 Hs.21861.0 NP_689491 Chr: xq22.1 0.45633 1.051298 MGC23947

729 211735_x_at SFTPC Surfactant, pulmonary- BC005913 gl 3543508 NP„003009 Chr:8p21 0.4563 0.095261 associated protein C /// Surfactant, pulmoπary- associated protein C729 211735_x_at SFTPC surfactant, pulmonary BC005913 gl 3543508 NP " 003009 Chr: 8p21 0.4563 0.095261 associated protein C /// surfactant, pulmonary-associated protein C

730 228766_at CD36 CD36 antigen (collagen AW299226 Hs.325823.0 NP_000063 Chr:7q11.2 0.45628 0.625663 Ol type I receptor, thrombospondin receptor)730 228766_at CD36 CD36 antigen (collagen AW299226 Hs.325823.0 NP_000063 Chr: 7q11.2 0.45628 0.625663 Ol type I receptor, thrombospondin receptor)

731 220161 s at EPB41L4B erythrocyte membrane NM_019114 g9506568 NP_060894 Chr:9q31-q32 0.4562 1.901012 protein band 4.1 like 4B /// erythrocyte membrane protein band 4.1 like 4B731 220161 s at EPB41L4B erythrocyte membrane NM_019114 g9506568 NP_060894 Chr: 9q31-q32 0.4562 1.901012 protein band 4.1 like 4B /// erythrocyte membrane protein band 4.1 like 4B

732 227188. _at C21orf63 chromosome 21 open AI744591 Hs.30156.0 NP. _478067 Chr:21q22.11 0.45578 0.095261 reading frame 63732 227188. _at C21orf63 chromosomes 21 open AI744591 Hs.30156.0 NP. _478067 Chr: 21q22.11 0.45578 0.095261 reading frame 63

733 200962 at RPL31 ribosomal protein L31 AI348010 Hs.184014.0 NP 000984 Chr:2q12.1 0.45568 0.095261733 200962 at RPL31 ribosomal protein L31 AI348010 Hs.184014.0 NP 000984 Chr: 2q12.1 0.45568 0.095261

734 229084 at CNTN4 contactin 4 R42166 Hs.94000.0 NP 783200 Chr:3p26-p25 0.45549 12.80219734 229084 at CNTN4 contact in 4 R42166 Hs.94000.0 NP 783200 Chr: 3p26-p25 0.45549 12.80219

735 224822_ _at DLC1 deleted in liver Cancer AA524250 Hs.8700.1 NP. _006085 Chr:8p22 0.4553 0.095261 1 735 224822_ _at DLC1 deleted in liver cancer AA524250 Hs.8700.1 NP. _006085 Chr: 8p22 0.4553 0.095261 1

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

736 210390_s_at CCL15 chemokine (C-C motif) AF031587 g2739163 NP_004158 Chr:17q11.2 0.45525 2.489117 ligand 15 /// chemokine (C-C motif) ligand 15736 210390_s_at CCL15 chemokine (C-C motif) AF031587 g2739163 NP_004158 Chr: 17q11.2 0.45525 2.489117 ligand 15 /// chemokine (C-C motif) ligand 15

737 239297 at Transcribed sequence BE932011 Hs.121518.0 0.45414 9.266777 with strong similarity to protein sp:P00722 (E. coli) BGALJ≡COLI Beta-galactosidase737 239297 at Transcribed sequence BE932011 Hs.121518.0 0.45414 9.266777 with strong similarity to protein sp: P00722 (E. coli) BGALJ≡COLI Beta-galactosidase

738 209543_s_at CD34 CD34 antigen /// CD34 M81104 g180108 NP. _001764 Chr:1q32 0.45302 0.095261 antigen738 209543_s_at CD34 CD34 antigen /// CD34 M81104 g180108 NP . _001764 Chr: 1q32 0.45302 0.095261 antigen

739 202242_at TM4SF2 transmembrane 4 NM_004615 g4759235 NP. _004606 Chr:xp11.4 0.453 9.625715 superfamily member 2 /// transmembrane 4 superfamily member 2739 202242_at TM4SF2 transmembrane 4 NM_004615 g4759235 NP . _004606 Chr: xp11.4 0.453 9.625715 superfamily member 2 /// transmembrane 4 superfamily member 2

740 208514 at KCNE1 potassium voltage- NM 000219 g4557686 NP 000210 Chr:21q22.12 0.45256 0.095261 gated Channel, Isk- related family, member 1 /// potassium voltage- uι740 208514 at KCNE1 potassium voltage NM 000219 g4557686 NP 000210 Chr: 21q22.12 0.45256 0.095261 gated Channel, Isk-related family, member 1 /// potassium voltage- uι

-4 gated Channel, Isk- related family, member 1-4 gated Channel, Isk-related family, member 1

741 225232 at PIP3AP phosphatidylinositol-3- AA524700 Hs.93872.1 NP_061934 Chr:5p13.3 0.45251 0.095261 phosphate associated protein741 225232 at PIP3AP Phosphatidylinositol-3 AA524700 Hs.93872.1 NP_061934 Chr: 5p13.3 0.45251 0.095261 Phosphorylated protein

742 205422 s at ITGBL1 integrin, beta-like 1 NM 004791 g4758613 NP_004782 Chr:13q33 0.45231 1.380569 (with EGF-like repeat domains) /// integrin, beta-like 1 (with EGF- like repeat domains)742 205422 s at ITGBL1 integrin, beta-like 1 NM 004791 g4758613 NP_004782 Chr: 13q33 0.45231 1.380569 (with EGF-like repeat domains) /// integrin, beta-like 1 (with EGF-like repeat domains)

743 228224. _at PRELP proline arginine-rich AA573140 Hs.183860.1 NP. _002716 Chr:1q32 0.45228 1.380569 end leucine-rich repeat protein743 228224. _at PRELP proline arginine-rich AA573140 Hs.183860.1 NP . _002716 Chr: 1q32 0.45228 1.380569 end leucine-rich repeat protein

744 204175. _at ZNF593 zinc finger protein 593 NM_015871 g7705661 NP_ .056955 Chr:1p35.3 0.45227 0.095261 /// zinc finger protein 593 744 204175. ZnF593 zinc finger protein 593 NM_015871 g7705661 NP_ .056955 Chr: 1p35.3 0.45227 0.095261 /// zinc finger protein 593

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change ""-Sample Number ID Protein ID Localization Change "" -

145 203140 at BCL6 B-cell CLL/lymphoma 6 NM_001706 g4502382 NP_001697 Chr:3q27 0.45204 0.149911 (zinc finger protein 51 ) /// B-cell145 203140 at BCL6 B-cell CLL / lymphoma 6 NM_001706 g4502382 NP_001697 Chr: 3q27 0.45204 0.149911 (zinc finger protein 51) /// B-cell

CLL/lymphoma 6 (zinc finger protein 51)CLL / lymphoma 6 (zinc finger protein 51)

746 209834_ _at CHST3 carbohydrate AB017915 g4115403 NP_ _004264 Chr:10q22.2 0.45201 1.051298 (chondroitin 6) sulfotransferase 3746 209834_ _at CHST3 carbohydrate AB017915 g4115403 NP_ _004264 Chr: 10q22.2 0.45201 1.051298 (chondroitin 6) sulfotransferase 3

747 227662 at AA541622 Hs.47447.0 0.45173 3.62324747 227662 at AA541622 Hs.47447.0 0.45173 3.62324

748 228971 at Transcribed sequence AI357655 Hs.292931.0 — 0.45163 0.149911 with weak similarity to protein sp:P39192748 228971 at Transcribed sequence AI357655 Hs.292931.0 - 0.45163 0.149911 with weak similarity to protein sp: P39192

(H.sapiens)(H.sapiens)

ALU5_HUMAN AIu subfamily SC sequence contamination waming entryALU5_HUMAN AI subfamily SC sequence contamination waming entry

749 213309 at Hs.54886.0 0.45156 0.201325749 213309 at Hs.54886.0 0.45156 0.201325

750 209894_at LEPR leptin receptor /// leptin U50748 g3236285 NP_002294 Chr:1p31 0.45048 0.201325 00 receptor750 209894_at LEPR leptin receptor /// leptin U50748 g3236285 NP_002294 Chr: 1p31 0.45048 0.201325 00 receptor

751 239806_at ARFIP1 ADP-ribosylation factor BF508641 Hs.136017.0 NP_055262 Chr:4q31.3 0.45032 9.266777 interacting protein 1 (arfaptin 1 )751 239806_at ARFIP1 ADP-ribosylation factor BF508641 Hs.136017.0 NP_055262 Chr: 4q31.3 0.45032 9.266777 interacting protein 1 (arfaptin 1)

752 227197_at SGEF Src homology 3 A1989530 Hs.12891.0 NPJ)56410 Chr:3q25.2 0.44989 13.5285 domain-containing gυanine nucleotide exchange factor752 227197_at SGEF Src homology 3 A1989530 Hs.12891.0 NPJ) 56410 Chr: 3q25.2 0.44989 13.5285 domain-containing a nucleotide exchange factor

753 219115_s_at IL20RA interleukin 20 receptor, NMJ) 14432 g7657690 NP_055247 Chr:6q22.33- 0.44975 2.08989 alpha /// interleukin 20 q23.1 receptor, alpha753 219115_s_at IL20RA interleukin 20 receptor, NMJ) 14432 g7657690 NP_055247 Chr: 6q22.33- 0.44975 2.08989 alpha /// interleukin 20 q23.1 receptor, alpha

754 226322 at ARG99 ARG99 protein BF109231 Hs.10683.0 NP 114126 Chr:12p11.23 0.44945 0.353669754 226322 at ARG99 ARG99 protein BF109231 Hs.10683.0 NP 114126 Chr: 12p11.23 0.44945 0.353669

755 215054_at EPOR erythropoietin receptor H16758 Hs.89548.1 NP_000112 Chr:19p13.3- 0.44932 12.53374 P13.2755 215054_at EPOR erythropoietin receptor H16758 Hs.89548.1 NP_000112 Chr: 19p13.3- 0.44932 12.53374 P13.2

756 229659_s_at PIGR Polymerie BE501712 Hs.205126.1 NPJ302635 Chr.iq31-q41 0.44924 0.518167 immunoglobulin receptor 756 229659_s_at PIGR Polymer BE501712 Hs.205126.1 NPJ302635 Chr.iq31-q41 0.44924 0.518167 immunoglobulin receptor

Nr. Aflymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Aflymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

757 229797_at MCOLN3 mucolipin 3 AI636080 Hs.49344.1 NP 060768 Chr:1 p22.3 0.44923 4.75532757 229797_at MCOLN3 mucolipin 3 AI636080 Hs.49344.1 NP 060768 Chr: 1 p22.3 0.44923 4.75532

758 205638_at BAI3 brain-specific NM_001704 g4502358 NP_001695 Chr:6q12 0.44834 11.06357 angiogenesis inhibitor 3 /// brain-specific angiogenesis inhibitor 3758 205638_at BAI3 brain-specific NM_001704 g4502358 NP_001695 Chr: 6q12 0.44834 11.06357 angiogenesis inhibitor 3 /// brain-specific angiogenesis inhibitor 3

759 221507_at BG258639 Hs.278378.1 0.44821 0.095261759 221507_at BG258639 Hs.278378.1 0.44821 0.095261

760 223938_at gl 2052909 0.44816 0.095261760 223938_at gl 2052909 0.44816 0.095261

761 213415_at CLIC2 chloride intracellular AI768628 Hs.54570.0 NP_001280 Chr:xq28 0.44789 1.702877 Channel 2761 213415_at CLIC2 chloride intracellular AI768628 Hs.54570.0 NP_001280 Chr: xq28 0.44789 1.702877 Channel 2

762 236296_x_at PDLIM2 PDZ and LIM domain 2 AW295176 Hs.197445.0 NP_067643 Chr:8p21.2 0.44731 0.095261 (mystique)762 236296_x_at PDLIM2 PDZ and LIM domain 2 AW295176 Hs.197445.0 NP_067643 Chr: 8p21.2 0.44731 0.095261 (mystique)

763 213285 at Hs.85335.0 0.44703 0.095261763 213285 at Hs.85335.0 0.44703 0.095261

764 228528_at — CDNA FLJ41270 fis, AI927692 Hs.290825.0 — — 0.44694 4.75532 clone BRAMY2036387764 228528_at - CDNA FLJ41270 fis, AI927692 Hs.290825.0 - - 0.44694 4.75532 clone BRAMY2036387

765 241544_at — Transcribed sequences R43301 Hs.22930.0 — — 0.44693 0.095261765 241544_at - Transcribed sequences R43301 Hs.22930.0 - - 0.44693 0.095261

766 241866_at SLC16A7 solute carrier family 16 AW975728 Hs.170504.0 NP_004722 Chr:12q13 0.4469 0.625663 (monocarboxyüc acid transporters), member 7766 241866_at SLC16A7 solute carrier family 16 AW975728 Hs.170504.0 NP_004722 Chr: 12q13 0.4469 0.625663 (monocarboxy acid transporters), member 7

767 224461 s at AMID apoptosis-inducing BC006121 gl 3543963 NP_116186 Chr:10q22.2 0.44669 0.095261 factor (AIF)-Iike mitochondrion- associated inducer of death /// apoptosis- inducing factor (AIF)- like mitochondrion- associated inducer of

Figure imgf000060_0001
767 224461 s at AMID apoptosis-inducing BC006121 gl 3543963 NP_116186 Chr: 10q22.2 0.44669 0.095261 factor (AIF) -Iike mitochondrion-associated inducer of death /// apoptosis-inducing factor (AIF) - like mitochondrion-associated inducer of
Figure imgf000060_0001

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change <0/-Sample ID ID Protein ID Localization Change < 0 / -

768 239252 at Transcribed sequence AA911561 Hs.123679.0 0.44611 0.518167 with weak similarity to protein sp:P39194 (H.sapiens) ALU7_HUMAN AIu subfamily SQ sequence contamination warning entry739 239252 at Transcribed sequence AA911561 Hs.123679.0 0.44611 0.518167 with weak similarity to protein sp: P39194 (H.sapiens) ALU7_HUMAN AI subfamily SQ sequence contamination warning entry

769 219213_at JAM2 junctional adhesion NM_021219 gl 0864028 NP_067042 Chr:21q21.2 0.44592 1.702877 moiecule 2 /// junctional adhesion moiecule 2769 219213_at JAM2 junctional adhesion NM_021219 gl 0864028 NP_067042 Chr: 21q21.2 0.44592 1.702877 moiecule 2 /// junctional adhesion moiecule 2

770 216912_at ARHGEF4 Rho guanine nucleotide AB029035 Hs.6066.2 NP_056135 Chr:2q22 0.44541 0.095261 exchange factor (GEF) 4770 216912_at ARHGEF4 Rho guanine nucleotide AB029035 Hs.6066.2 NP_056135 Chr: 2q22 0.44541 0.095261 exchange factor (GEF) 4

771 220835 s at ZNF407 zinc finger protein 407 NM_017757 g8923288 NP 060227 Chr:18q23 0.4452 1.051298771 220835 s at ZNF407 zinc finger protein 407 NM_017757 g8923288 NP 060227 Chr: 18q23 0.4452 1.051298

772 201041_s_at DUSP1 dual specificity NM_004417 g7108342 NP_004408 Chr:5q34 0.44502 0.095261 Phosphatase 1 /// dual O specificity Phosphatase 1772 201041_s_at DUSP1 dual specificity NM_004417 g7108342 NP_004408 Chr: 5q34 0.44502 0.095261 Phosphatase 1 /// dual O specificity Phosphatase 1

773 227551_at C9orf77 chromosome 9 open BE856596 Hs.91052.0 NP_057098 Chr:9q21.2 0.445 0.095261 reading frame 77773 227551_at C9orf77 chromosome 9 open BE856596 Hs.91052.0 NP_057098 Chr: 9q21.2 0.445 0.095261 reading frame 77

774 214638_s_at CCNT2 cyclin T2 AV681875 Hs.155478.2 NP 001232 Chr:2q21.3 0.44431 8.153992774 214638_s_at CCNT2 cyclin T2 AV681875 Hs.155478.2 NP 001232 Chr: 2q21.3 0.44431 8.153992

775 222784_at SMOC1 SPARC related AJ249900 Hs.14144.0 NP_071420 Chr:14q24.1 0.44418 1.223189 modular calcium binding 1775 222784_at SMOC1 SPARC related AJ249900 Hs.14144.0 NP_071420 Chr: 14q24.1 0.44418 1.223189 modular calcium binding 1

776 227099_s_at LOC387763 hypothetical AW276078 Hs.127824.0 — Chr:11p11.2 0.44412 0.095261 LOC387763776 227099_s_at LOC387763 hypothetical AW276078 Hs.127824.0 - Chr: 11p11.2 0.44412 0.095261 LOC387763

777 217534_at BM-009 hypothetical protein AA845825 Hs.282721.0 NP_057707 Chr:8q24.21 0.44317 0.625663 BM-009777 217534_at BM-009 hypothetical protein AA845825 Hs.282721.0 NP_057707 Chr: 8q24.21 0.44317 0.625663 BM-009

778 221246_x_at TNS tensin /// tensin /// NM_018274 gl 3775159 NP_072174 Chr.2q35-q36 0.44309 0.353669 tensin778 221246_x_at TNS tensin /// tensin /// NM_018274 gl 3775159 NP_072174 Chr.2q35-q36 0.44309 0.353669 tensin

779 232606_at — CDNA FLJ11832 fis, AK021894 Hs.146014.0 — — 0.44308 0.353669 clone HEMBA1006566779 232606_at - CDNA FLJ11832 fis, AK021894 Hs.146014.0 - - 0.44308 0.353669 clone HEMBA1006566

780 230958_s_at MRNA BE670797 Hs.12482.3 0.44302 0.095261 780 230958_s_at MRNA BE670797 Hs.12482.3 0.44302 0.095261

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

781 211598_x_at VIPR2 vasoactive intestinal U18810 gl 684934 NP_003373 Chr:7q36.3 0.44297 0.518167 peptide receptor 2 /// vasoactive intestinal peptide receptor 2781 211598_x_at VIPR2 vasoactive intestinal U18810 gl 684934 NP_003373 Chr: 7q36.3 0.44297 0.518167 peptide receptor 2 /// vasoactive intestinal peptide receptor 2

782 212730 at DMN desmuslin AK026420 Hs.10587.0 NP 056101 Chr:15q26.3 0.44291 2.08989782 212730 at DMN desmuslin AK026420 Hs.10587.0 NP 056101 Chr: 15q26.3 0.44291 2.08989

783 224832_at DUSP16 dual specificity AB051487 Hs.20281.0 NP_085143 Chr:12p13 0.44264 0.095261 Phosphatase 16783 224832_at DUSP16 dual specificity AB051487 Hs.20281.0 NP_085143 Chr: 12p13 0.44264 0.095261 Phosphatase 16

784 207761_s_at DKFZP586A0522 DKFZP586A0522 NM_014033 gl 3378140 NP_054752 Chr:12q13.13 0.44258 6.225758 protein784 207761_s_at DKFZP586A0522 DKFZP586A0522 NM_014033 gl 3378140 NP_054752 Chr: 12q13.13 0.44258 6.225758 protein

785 235979_at C7 complement AI697588 Hs.126528.0 NP_000578 Chr:5p13 0.44234 9.266777 component 7785 235979_at C7 complement AI697588 Hs.126528.0 NP_000578 Chr: 5p13 0.44234 9.266777 component 7

786 225575_at AI680541 Hs.23767.2 0.44215 1.051298786 225575_at AI680541 Hs.23767.2 0.44215 1.051298

787 226303_at Hs.46531.0 0.44199 1.380569787 226303_at Hs.46531.0 0.44199 1.380569

788 215008_at TLL2 tolloid-like 2 AA582404 Hs.154296.0 NP_036597 Chr:10q23-q24 0.44152 0.095261788 215008_at TLL2 tolloid-like 2 AA582404 Hs.154296.0 NP_036597 Chr: 10q23-q24 0.44152 0.095261

789 244864 at Transcribed sequence W19283 Hs.169977.0 — 0.44118 11.55252 with weak similarity to protein sp:P39193 ON (H.sapiens) ALU6_HUMAN AIu subfamily SP sequence contamination warπing entry789 244864 at Transcribed sequence W19283 Hs.169977.0 - 0.44118 11.55252 with weak similarity to protein sp: P39193 ON (H.sapiens) ALU6_HUMAN AI subfamily SP sequence contamination warping entry

790 243141_at D31421 Hs.62666.0 0.44077 2.08989790 243141_at D31421 Hs.62666.0 0.44077 2.08989

791 220398 at MGC4170 MGC4170 protein NM 024312 gl 3236550 NP 077288 Chr:12q23.3 0.44071 0.095261791 220398 at MGC4170 MGC4170 protein NM 024312 gl 3236550 NP 077288 Chr: 12q23.3 0.44071 0.095261

792 221886_at KIAA1277 KIAA1277 AL037701 Hs.6385.0 — Chr:7q34 0.44032 3.612633792 221886_at KIAA1277 KIAA1277 AL037701 Hs.6385.0 - Chr: 7q34 0.44032 3.612633

793 228098_s_at MYLIP myosin regulatory light AW292746 Hs.20072.2 NP. _037394 Chr:6p23-p22.3 0.43989 0.095261 chain interacting protein793 228098_s_at MYLIP myosin regulatory light AW292746 Hs.20072.2 NP . _037394 Chr: 6p23-p22.3 0.43989 0.095261 chain-interacting protein

794 230511_at CREM cAMP responsive AI800640 Hs.167916.0 NP. ,001872 Chr:10p11.21 0.43984 2.08989 element modulator794 230511_at CREM cAMP responsive AI800640 Hs.167916.0 NP . , 001872 Chr: 10p11.21 0.43984 2.08989 element modulator

795 243278 at — Transcribed sequences AW291402 Hs.149057.0 — — 0.43975 3.306454795 243278 at - Transcribed sequences AW291402 Hs.149057.0 - - 0.43975 3.306454

796 215726_s_at CYB5 cytochrome b-5 /// M22976 Hs.83834.2 NP. _001905 Chr:18q23 0.43923 0.095261 cytochrome b-5 796 215726_s_at CYB5 cytochrome b-5 /// M22976 Hs.83834.2 NP . _001905 Chr: 18q23 0.43923 0.095261 cytochrome b-5

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

797 20561 O_at MYOM1 myomesin 1 (skelemin) NM_003803 g4505312 NP_003794 Chr:18p11.32- 0.43923 4.436744 185kDa /// myomesin 1 p11.31 (skelemin) 185kDa797 20561 O_at MYOM1 myomesin 1 (skelemin) NM_003803 g4505312 NP_003794 Chr: 18p11.32- 0.43923 4.436744 185kDa /// myomesin 1 p11.31 (skelemin) 185kDa

798 202746_at - ITM2A integral membrane AL021786 Hs.17109.0 NP_004858 Chr:xq13.3- 0.43858 1.380569 protein 2A xq21.2798 202746_at - ITM2A integral membrane AL021786 Hs.17109.0 NP_004858 Chr: xq13.3- 0.43858 1.380569 protein 2A xq21.2

799 208323_s_at ANXA13 annexin A13 /// annexin NMJD04306 g4757753 NP_004297 Chr:8q24.1- 0.43775 9.625715 A13 q24.2799 208323_s_at ANXA13 annexin A13 /// annexin NMJD04306 g4757753 NP_004297 Chr: 8q24.1- 0.43775 9.625715 A13 q24.2

800 201348_at GPX3 glutathione peroxidase NM_002084 g6006000 NP_002075 Chr:5q23 0.43775 2.489117 3 (plasma) /// glutathione peroxidase 3 (plasma)800 201348_at GPX3 glutathione peroxidase NM_002084 g6006000 NP_002075 Chr: 5q23 0.43775 2.489117 3 (plasma) /// glutathione peroxidase 3 (plasma)

801 23971 O_at — Transcribed sequences AA993515 Hs.99283.0 — — 0.43764 0.201325801 23971 O_at - Transcribed sequences AA993515 Hs.99283.0 - - 0.43764 0.201325

802 239757_at Clone AA741493 Hs.143842.0 0.43687 0.095261 IMAGE:4716331 , mRNA802 239757_at Clone AA741493 Hs.143842.0 0.43687 0.095261 IMAGE: 4716331, mRNA

803 209335_at DCN decorin AI281593 Hs.76152.1 NP_001911 Chr:12q13.2 0.43665 1.380569803 209335_at DCN decorin AI281593 Hs.76152.1 NP_001911 Chr: 12q13.2 0.43665 1.380569

804 228376_at a1/3GTP alpha-1 ,3- AI972498 Hs.97469.0 Chr:9q34.11 0.43647 0.201325 galactosyltransferase pseudogene804 228376_at a1 / 3GTP alpha-1, 3- AI972498 Hs.97469.0 Chr: 9q34.11 0.43647 0.201325 galactosyltransferase pseudogenes

805 236396_at — Transcribed sequences AW051952 Hs.136591.0 — — 0.43642 0.518167805 236396_at - Transcribed sequences AW051952 Hs.136591.0 - - 0.43642 0.518167

806 202063_s_at SEL1L sel-1 suppressor of lin- AB020335 g6518494 NP_005056 Chr:14q24.3- 0.43601 0.095261 12-like (C. elegans) q31806 202063_s_at SEL1L sel-1 suppressor of lin AB020335 g6518494 NP_005056 Chr: 14q24.3- 0.43601 0.095261 12-like (C. elegans) q31

807 241337_at Hypothetical AI498602 Hs.157864.0 Chr:10q11.21 0.43587 0.095261 LOC283034 (LOC283034), mRNA807 241337_at Hypothetical AI498602 Hs.157864.0 Chr: 10q11.21 0.43587 0.095261 LOC283034 (LOC283034), mRNA

808 217442_at Hs.274514.0 0.43578 0.353669808 217442_at Hs.274514.0 0.43578 0.353669

809 205357_s_at AGTR1 angiotensin Il receptor, NMJJ00685 g6715581 NP_000676 Chr:3q21-q25 0.43559 0.353669 type 1 /// angiotensin Il receptor, type 1809 205357_s_at AGTR1 angiotensin II receptor, NMJJ00685 g6715581 NP_000676 Chr: 3q21-q25 0.43559 0.353669 type 1 /// angiotensin IL receptor, type 1

810 213992_at COL4A6 Collagen, type IV, alpha AI889941 Hs.408.2 NP_001838 Chr:xq22 0.43555 0.518167 6 810 213992_at COL4A6 Collagen, type IV, alpha AI889941 Hs.408.2 NP_001838 Chr: xq22 0.43555 0.518167 6

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

811 203408„s_at SATB1 special AT-rich NM„002971 g4506790 NP_002962 Chr:3p23 0.4354 0.095261 sequence binding protein 1 (binds to nuciear matrix/scaffold- associating DNA's) /// special AT-rich sequence binding protein 1 (binds to nuciear matrix/scaffold- associating DNA's)811 203408 "s_at SATB1 special AT-rich NM " 002971 g4506790 NP_002962 Chr: 3p23 0.4354 0.095261 Sequence binding protein 1 (binds to nuclear matrix / scaffold-associating DNA's) /// special AT-rich sequence binding protein 1 (binds to nuciear matrix / scaffold-associating DNA's)

812 223395_at TARSH target of Nesh-SH3 AB056106 gl 3111479 NP 056244 Chr:3q12 0.43473 11.55252812 223395_at TARSH target of Nesh-SH3 AB056106 gl 3111479 NP 056244 Chr: 3q12 0.43473 11.55252

813 229485_x_at LOC152573 hypothetical protein AI735586 Hs.103819.0 — Chr:4p14 0.43456 2.489117 BC012029813 229485_x_at LOC152573 hypothetical protein AI735586 Hs.103819.0 - Chr: 4p14 0.43456 2.489117 BC012029

814 225817_at Hs.10119.0 0.43372 0.095261814 225817_at Hs.10119.0 0.43372 0.095261

815 220832_at TLR8 toil-like receptor 8 /// NM_016610 g7706147 NP_057694 Chr:xp22 0.43345 1.051298 σ

Figure imgf000064_0001
\ toll-like receptor 8815 220832_at TLR8 toil-like receptor 8 /// NM_016610 g7706147 NP_057694 Chr: xp22 0.43345 1.051298 σ
Figure imgf000064_0001
\ toll-like receptor 8

816 204497_at ADCY9 adenylate cydase 9 /// AB011092 Hs.20196.0 NP_001107 Chr:16p13.3 0.43344 0.095261 adenylate cyclase 9816 204497_at ADCY9 adenylate cydase 9 /// AB011092 Hs.20196.0 NP_001107 Chr: 16p13.3 0.43344 0.095261 adenylate cyclase 9

817 240379_at — Transcribed sequences AA845608 Hs.132860.0 — — 0.4332 0.095261817 240379_at - Transcribed sequences AA845608 Hs.132860.0 - - 0.4332 0.095261

818 213541_s_at ERG v-ets erythroblastosis AI351043 Hs.279477.0 NP_004440 Chr:21q22.3 0.43295 0.149911 virus E26 oncogene like (avian)818 213541_s_at ERG v-ets erythroblastosis AI351043 Hs.279477.0 NP_004440 Chr: 21q22.3 0.43295 0.149911 virus E26 oncogene like (avian)

819 221667_s_at g5901654 0.43247 2.489117819 221667_s_at g5901654 0.43247 2.489117

820 223315 at NTN4 netrin 4 AF278532 gl 1120047 NP 067052 Chr:12q22-q23 0.4321 0.095261820 223315 at NTN4 netrin 4 AF278532 gl 1120047 NP 067052 Chr: 12q22-q23 0.4321 0.095261

821 209708_at MOXD1 monooxygenase, DBH- AY007239 g9988949 NP_056344 Chr:6q23.1-23.3 0.43172 1.380569 iϊke 1821 209708_at MOXD1 monooxygenase, DBH-AY007239 g9988949 NP_056344 Chr: 6q23.1-23.3 0.43172 1.380569 iϊke 1

822 210692_s_at SLC43A3 solute carrier family 43, BC003163 gl 3111980 NPJ554815 Chr:11q11 0.43168 0.353669 member 3822 210692_s_at SLC43A3 solute carrier family 43, BC003163 gl 3111980 NPJ554815 Chr: 11q11 0.43168 0.353669 member 3

823 204381_at LRP3 low density lipoprotein NM_002333 g4505014 NP_002324 Chr:19q13.12 0.43086 11.70204 receptor-related protein 3 /// low density lipoprotein receptor- related protein 3823 204381_at LRP3 low density lipoprotein NM_002333 g4505014 NP_002324 Chr: 19q13.12 0.43086 11.70204 receptor-related protein 3 /// low-density lipoprotein receptor-related protein 3

824 234497_s_at CDNA FLJ12051 fis, AK022113 Hs.301858.1 0.43078 0.095261 clone HEMBB1002005 824 234497_s_at CDNA FLJ12051 fis, AK022113 H.3301858.1 0.43078 0.095261 clone HEMBB1002005

-• Affymetrix Gensymbol Genname Repräsentative Transkrtpt ID Repräsentative Chromosomale FoId q-value Probennummer IO Protein ID Lokalisierung Change- • Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number IO Protein ID Localization Change

825 232449_at BCDO2 beta-caroteπe AV710838 Hs.181795.0 NPJ 14144 Chr:11q22.3- 0.43064 0.779487 dioxygenase 2 q23.1825 232449_at BCDO2 beta-carotene AV710838 Hs.181795.0 NPJ 14144 Chr: 11q22.3- 0.43064 0.779487 dioxygenase 2 q23.1

826 204796„at EML1 echinoderm AI825937 Hs.12451.0 NPJ504425 Chr:14q32 0.43063 0.353669 microtubuie associated protein üke 1826 204796 " at EML1 echinoderm AI825937 Hs.12451.0 NPJ504425 Chr: 14q32 0.43063 0.353669 microtubule associated protein

827 227702_at CYP4X1 cytochrorne P450, M557324 Hs.26040.0 NP_828847 Chr:1p33 0.42887 0.518167 family 4, subfamily X, Polypeptide 1827 227702_at CYP4X1 cytochrome P450, M557324 Hs.26040.0 NP_828847 Chr: 1p33 0.42887 0.518167 family 4, subfamily X, polypeptides 1

828 229649_at NRXN3 neurexirt 3 AH 29949 Hs.22269.1 NP 004787 Chr;14q31 0.42851 1.901012828 229649_at NRXN3 newly 3 AH 29949 Hs.22269.1 NP 004787 Chr; 14q31 0.42851 1.901012

829 217044_s_at KIAA0599 KIAA0599 BC004298 Hs.198037.2 — Chr:14q23.3 0.42798 0.095261829 217044_s_at KIAA0599 KIAA0599 BC004298 Hs.198037.2 - Chr: 14q23.3 0.42798 0.095261

830 211039 at CHRNA1 choünergic receptor, BC006314 gl 3623428 NP_000070 Chr:2q24-q32 0.42767 0.095261 nicotinic, alpha Polypeptide 1 (muscle) /// choünergic receptor, nicotinic, alpha Polypeptide 1 (muscle)830 211039 at CHRNA1 choünergic receptor, BC006314 gl 3623428 NP_000070 Chr: 2q24-q32 0.42767 0.095261 nicotinic, alpha polypeptides 1 (muscle) /// choünergic receptor, nicotinic, alpha polypeptides 1 (muscle)

831 217022_s_at MGC27165 hypothetical protein S55735 Hs.293441.3 — Chr:14q32.33 0.42742 2.814919 MGC27165831 217022_s_at MGC27165 hypothetical protein S55735 Hs.293441.3 - Chr: 14q32.33 0.42742 2.814919 MGC27165

832 228218 at Hs.26409.0 0.427 0.353669832 228218 at Hs.26409.0 0.427 0.353669

833 226145 s at Hs.15420.1 0.4262 9.266777833 226145 s at Hs.15420.1 0.4262 9.266777

834 235494_at Clone AA702209 Hs.288405.0 -- 0.42595 13.5285 IMAGE:5278074, mRNA834 235494_at Clone AA702209 Hs.288405.0 - 0.42595 13.5285 IMAGE: 5278074, mRNA

835 234978_at SLC36A4 solυte carrier famüy 36 AL542358 Hs.292653.0 NP_689526 Chr:11q14.3 0.42564 0.095261 (proton/amino acid symporter), rnember 4835 234978_at SLC36A4 solυte carrier famuy 36 AL542358 Hs.292653.0 NP_689526 Chr: 11q14.3 0.42564 0.095261 (proton / amino acid symporter), November 4

836 220446 s at CHST4 carbohydrate (N- NMJ305769 g5031734 NP_005760 Chr:16q22.2 0.42547 3.612633 acetylglucosamine 6-0) sulfotransferase 4 /// carbohydrate (N- acetylglυcosamine 6-0) sulfotransferase 4836 220446 s at CHST4 carbohydrate (N-NMJ305769 g5031734 NP_005760 Chr: 16q22.2 0.42547 3.612633 acetylglucosamine 6-0) sulfotransferase 4 /// carbohydrate (N-acetylglucosamine 6-0) sulfotransferase 4

837 241365„at — CDNA FLJ42259 fis, AA002140 Hs.33024.0 — — 0.42543 0.518167 clone TKIDN2011289837 241365 "at - CDNA FLJ42259 fis, AA002140 Hs.33024.0 - - 0.42543 0.518167 clone TKIDN2011289

838 224703__at WDR22 WD repeat domain 22 AI814644 Hs.284294.0 — Cnr:14q23~ 0.42512 0.095261 q24.1 838 224703__at WDR22 WD repeat domain 22 AI814644 Hs.284294.0 - Cnr: 14q23 ~ 0.42512 0.095261 q24.1

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

839 224973_at C6orf37 chromosome 6 open AL078599 Hs.10784.3 NP_060103 Chr:6q14 0.42454 0.779487 reading frame 37839 224973_at C6orf37 chromosomes 6 open AL078599 Hs.10784.3 NP_060103 Chr: 6q14 0.42454 0.779487 reading frame 37

840 234488_s_at GMCL1L germ cell-less homolog AF198534 Hs.302032.0 NP_071916 Chr:5q35.3 0.42396 1.051298 1 (Drosophila)-Iike840 234488_s_at GMCL1L germ cell-less homolog AF198534 Hs.302032.0 NP_071916 Chr: 5q35.3 0.42396 1.051298 1 (Drosophila) -Iike

841 204688_at SGCE sarcoglycan, epsilon /// NM_003919 gl 0835046 NP_003910 Chr:7q21-q22 0.42325 0.095261 sarcoglycan, epsilon841 204688_at SGCE sarcoglycan, epsilon /// NM_003919 gl 0835046 NP_003910 Chr: 7q21-q22 0.42325 0.095261 sarcoglycan, epsilon

842 227669_at — Transcribed sequences AI654636 Hs.5794.0 — — 0.42286 0.149911842 227669_at - Transcribed sequences AI654636 Hs.5794.0 - - 0.42286 0.149911

843 219873_at COLEC11 collectin sub-family NM_024027 gl 3128971 NP_076932 Chr:2p25.3 0.42255 8.779357 member 11843 219873_at COLEC11 collectin sub-family NM_024027 gl 3128971 NP_076932 Chr: 2p25.3 0.42255 8.779357 member 11

844 206876_at AL121948 Hs.105925.0 0.4224 2.571322844 206876_at AL121948 Hs.105925.0 0.4224 2.571322

845 202967_at GSTA4 glutathione S- NM_001512 g4504172 NP_001503 Chr:6p12.1 0.42234 0.095261 transferase A4 /// glutathione S- transferase A4845 202967_at GSTA4 glutathione S- NM_001512 g4504172 NP_001503 Chr: 6p12.1 0.42234 0.095261 transferase A4 /// glutathione S-transferase A4

846 207220_at DO Dombrock blood group NM_021071 gl 2711661 NP_066549 Chr:12q13.2- 0.42215 0.518167 /// Dombrock blood q13.3 group846 207220_at DO Dombrock blood group NM_021071 gl 2711661 NP_066549 Chr: 12q13.2- 0.42215 0.518167 /// Dombrock blood q13.3 group

847 207367 at ATP12A ATPase, H+/K+ NM_001676 gl 0280617 NPJ301667 Chr.13q11- 0.42202 0.353669 Ul transporting, q12.1 nongastric, alpha Polypeptide /// ATPase, H+/K+ transporting, nongastric, alpha Polypeptide847 207367 ATP12A ATPase, H + / K + NM_001676 gl 0280617 NPJ301667 Chr.13q11- 0.42202 0.353669 Ul transporting, q12.1 nongastric, alpha polypeptides /// ATPase, H + / K + transporting, nongastric, alpha polypeptides

848 201914_s_at SEC63 SEC63-Iike (S. AK001465 Hs.31575.0 NP. _009145 Chr:6q21 0.42141 0.095261 cerevisiae)848 201914_s_at SEC63 SEC63-Iike (S. AK001465 Hs.31575.0 NP . _009145 Chr: 6q21 0.42141 0.095261 cerevisiae)

849 225539_at PRDM15 PR domain containing AP001745 Hs.157079.0 NP_ _071398 Chr:21q22.3 0.4214 0.149911 15849 225539_at PRDM15 PR domain containing AP001745 Hs.157079.0 NP_ _071398 Chr: 21q22.3 0.4214 0.149911 15

850 215440_s_at BEXL1 brain expressed X- AL523320 Hs.184736.1 — Chr:xq22.1- 0.42099 4.830969 linked-like 1 q22.3850 215440_s_at BEXL1 brain expressed X- AL523320 Hs.184736.1 - Chr: xq22.1- 0.42099 4.830969 linked-like 1 q22.3

851 210613 s at SYNGR1 synaptogyrin 1 BC000731 gl 2653878 NP 004702 Chr.22q13.1 0.42089 3.612633851 210613 s at SYNGR1 synaptogyrin 1 BC000731 gl 2653878 NP 004702 Chr.22q13.1 0.42089 3.612633

852 216209 at Hs.39337.0 0.42084 0.353669852 216209 at Hs.39337.0 0.42084 0.353669

853 206068_s_at ACADL acyl-Coenzyme A AI367275 Hs.1209.0 NP_ JD01599 Chr:2q34-q35 0.42073 11.06357 dehydrogenase, long chain853 206068_s_at ACADL acyl Coenzymes A AI367275 Hs.1209.0 NP_ JD01599 Chr: 2q34-q35 0.42073 11.06357 dehydrogenase, long chain

854 238778_at AI244661 Hs.103296.0 0.42036 0.201325 854 238778_at AI244661 Hs.103296.0 0.42036 0.201325

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung ChangeNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change

855 242358_at CDNA FLJ35666 fis, AW024656 Hs.233382.0 — — 0.41986 0.095261 clone SPLEN2017781855 242358_at CDNA FLJ35666 fis, AW024656 Hs.233382.0 - - 0.41986 0.095261 clone SPLEN2017781

856 239218_at Clone AI800515 Hs.12024.0 0.41979 2.489117 IMAGE:5288238, mRNA856 239218_at Clone AI800515 Hs.12024.0 0.41979 2.489117 IMAGE: 5288238, mRNA

857 226806 s at MRNA AW022607 Hs.12482.1 0.41976 0.095261857 226806 s at MRNA AW022607 Hs.12482.1 0.41976 0.095261

858 204894 s at AOC3 amine oxidase, copper NM_003734 g6806883 NP_003725 Chr:17q21 0.41945 3.62324 containing 3 (vascular adhesion protein 1) /// amine oxidase, copper containing 3 (vascular adhesion protein 1)Oxidase, copper containing 3 (vascular adhesion protein 1)

859 241386 at — — AW270845 Hs.133459.0 — — 0.41943 1.051298859 241386 at - - AW270845 Hs.133459.0 - - 0.41943 1.051298

860 211734_s_at gl 3543505 0.41886 6.225758860 211734_s_at gl 3543505 0.41886 6.225758

861 232090_at — CDNA FLJ11481 fis, . AI761578 Hs.135159.0 — — 0.41842 1.901012 clone HEMBA1001803861 232090_at - CDNA FLJ11481 fis,. AI761578 Hs.135159.0 - - 0.41842 1.901012 clone HEMBA1001803

862 205898_at CX3CR1 chemokine (C-X3-C U20350 g665580 NP_001328 Chr:3p21.3 0.41833 1.901012 motif) receptor 1 /// chemokine (C-X3-C motif) receptor 1862 205898_at CX3CR1 chemokines (C-X3-C U20350 g665580 NP_001328 Chr: 3p21.3 0.41833 1.901012 motif) receptor 1 /// chemokines (C-X3-C motif) receptor 1

863 223475 at LOC83690 CocoaCrisp AF142573 gl 2002310 NP 113649 Chr:8q13.3 0.41826 0.779487863 223475 at LOC83690 Cocoa Crisps AF142573 gl 2002310 NP 113649 Chr: 8q13.3 0.41826 0.779487

864 233051_at SLITRK2 SLIT and NTRK-Iike AL109653 Hs.21527.0 NPJ 15928 Chr:xq27.3 0.41823 0.095261 family, member 2864 233051_at SLITRK2 SLIT and NTRK-Iike AL109653 Hs.21527.0 NPJ 15928 Chr: xq27.3 0.41823 0.095261 family, member 2

865 235019_at CPM carboxypeptidase M BE878495 Hs.267158.0 NP 001865 Chr:12q14.3 0.4178 1.223189865 235019_at CPM carboxypeptidase M BE878495 Hs.267158.0 NP 001865 Chr: 12q14.3 0.4178 1.223189

866 220136_s_at CRYBA2 crystallin, beta A2 /// NM_005209 g7019356 NP_005200 Chr:2q34-q36 0.41707 0.095261 crystailin, beta A2866 220136_s_at CRYBA2 crystallin, beta A2 /// NM_005209 g7019356 NP_005200 Chr: 2q34-q36 0.41707 0.095261 crystailin, beta A2

867 214983_at Hs.45068.0 0.41676 0.518167867 214983_at Hs.45068.0 0.41676 0.518167

868 213317_at Hs.21103.0 0.41654 0.149911868 213317_at Hs.21103.0 0.41654 0.149911

869 238066_at RBP7 retinoi binding protein AI733027 Hs.292718.0 NP_443192 Chr:1p36.22 0.41652 2.489117 7, cellular869 238066_at RBP7 retinoi binding protein AI733027 Hs.292718.0 NP_443192 Chr: 1p36.22 0.41652 2.489117 7, cellular

870 51226_at LOC389677 simiiar to RIKEN cDNA N53536 4871749_RC NP_976324 Chr:8q22.1 0.416 0.149911 3000004N20870 51226_at LOC389677 simiiar to RIKEN cDNA N53536 4871749_RC NP_976324 Chr: 8q22.1 0.416 0.149911 3000004N20

871 205414_s_at KIAA0672 KIAA0672 gene NM_014859 g7662241 — Chr:17p12 0.41542 8.153992 product 871 205414_s_at KIAA0672 KIAA0672 gene NM_014859 g7662241 - Chr: 17p12 0.41542 8.153992 product

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung ChangeNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change

872 219718_at FLJ 10986 hypothetical protein NMJD18291 g8922805 NP_060761 Chr:1 p32.1 0.41507 4.436744 FLJ10986872 219718_at FLJ 10986 hypothetical protein NMJD18291 g8922805 NP_060761 Chr: 1 p32.1 0.41507 4.436744 FLJ10986

873 221627_at gl 2407414 0.41497 0.518167873 221627_at gl 2407414 0.41497 0.518167

874 215369_at — CDNA FLJ11396 fis, AU145354 Hs.287411.0 — — 0.41483 0.095261 clone HEMBA1000604874 215369_at - CDNA FLJ11396 fis, AU145354 Hs.287411.0 - - 0.41483 0.095261 clone HEMBA1000604

875 229544_at Clone AI690169 Hs.132882.0 0.41357 0.095261 IMAGE-.5315196, mRNA875 229544_at Clone AI690169 Hs.132882.0 0.41357 0.095261 IMAGE-.5315196, mRNA

876 207179_at TLX1 T-cell leukemia, NM_005521 g5031756 NP__005512 Chr:10q24 0.41323 0.201325 homeobox 1 /// T-cell leukemia, homeobox 1876 207179_at TLX1 T-cell leukemia, NM_005521 g5031756 NP__005512 Chr: 10q24 0.41323 0.201325 homeobox 1 /// T-cell leukemia, homeobox 1

877 203697_at FRZB frizzled-related protein U91903 gl 917006 NP_001454 Chr:2qter 0.41314 3.306454 /// frizzled-related protein877 203697_at FRZB frizzled-related protein U91903 gl 917006 NP_001454 Chr: 2qter 0.41314 3.306454 /// frizzled-related protein

878 207468_s_at SFRP5 secreted frizzled- NM_003015 g8400734 NP_003006 Chr:10q24.1 0.41279 0.095261 related protein 5 /// secreted frizzled- related protein 5 9\ -4878 207468_s_at SFRP5 secreted frizzled- NM_003015 g8400734 NP_003006 Chr: 10q24.1 0.41279 0.095261 related protein 5 /// secreted frizzled- related protein 5 9 \ -4

879 241934 at HNT neurotrimin BF378300 Hs.318494.0 NP 057606 Chr:11q25 0.41248 0.095261879 241934 at HNT neurotrimina BF378300 Hs.318494.0 NP 057606 Chr: 11q25 0.41248 0.095261

880 203088_at FBLN5 fibulin 5 /// fibulin 5 NM_006329 g5453649 NP_006320 Chr:14q32.1 0.41243 0.779487880 203088_at FBLN5 fibulin 5 /// fibulin 5 NM_006329 g5453649 NP_006320 Chr: 14q32.1 0.41243 0.779487

881 226492 at SEMA6D sema domaiπ, AL036088 Hs.191098.0 NPJ)65909 Chr:15q15.2 0.41123 13.5285 transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D881 226492 at SEMA6D sema domaiπ, AL036088 Hs.191098.0 NPJ) 65909 Chr: 15q15.2 0.41123 13.5285 transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D

882 207051 at SLC17A4 solute carrier family 17 NM_005495 g4885440 NPJ305486 Chr.6p22-p21.3 0.41121 3.612633882 207051 at SLC17A4 solute carrier family 17 NM_005495 g4885440 NPJ305486 Chr.6p22-p21.3 0.41121 3.612633

(sodium phosphate), member 4 /// solute carrier family 17 (sodium phosphate), member 4(sodium phosphate), member 4 /// solute carrier family 17 (sodium phosphate), member 4

883 235761. _at — CDNA FLJ36553 fis, BG111808 Hs.167167.0 — — 0.41066 0.353669 clone TRACH2008478883 235761. _at - CDNA FLJ36553 fis, BG111808 Hs.167167.0 - - 0.41066 0.353669 clone TRACH2008478

884 205328. .at CLDMO claudin 10 /// claudin 10 NM 006984 g5921464 NP_008915 Chr:13q31-q34 0.41062 0.095261884 205328.at CLDMO claudin 10 /// claudin 10 NM 006984 g5921464 NP_008915 Chr: 13q31-q34 0.41062 0.095261

885 229691. _at CDNA FLJ43013 fis, BF221887 Hs.170853.0 — Chr:14q32.33 0.41042 13.5285 clone BRTHA2016215 885 229691. _at CDNA FLJ43013 fis, BF221887 Hs.170853.0 - Chr: 14q32.33 0.41042 13.5285 clone BRTHA2016215

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung ChangeNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change

886 214321_at NOV nephroblastoma BF440025 Hs.235935.1 NP_002505 Chr:8q24.1 0.41027 11.70204 overexpressed gene886 214321_at NOV nephroblastoma BF440025 Hs.235935.1 NP_002505 Chr: 8q24.1 0.41027 11.70204 overexpressed gene

887 229530 at — Transcribed sequences BF002625 Hs.143691.0 — — 0.41012 0.779487887 229530 at - Transcribed sequences BF002625 Hs.143691.0 - - 0.41012 0.779487

888 207067_s_at HDC histidine decarboxylase NM_002112 g4504364 NP_002103 Chr:15q21-q22 0.40954 0.518167 /// histidine decarboxylase888 207067_s_at HDC histidine decarboxylase NM_002112 g4504364 NP_002103 Chr: 15q21-q22 0.40954 0.518167 /// histidine decarboxylase

889 221287 at RNASEL ribonuclease L (2',5'- NM_021133 gl 0863928 NP_066956 Chr:1q25 0.40937 0.095261 oügoisoadenylate synthetase-dependent)889 221287 at RNASEL ribonuclease L (2 ', 5'-NM_021133 gl 0863928 NP_066956 Chr: 1q25 0.40937 0.095261 oügoisoadenylate synthetase-dependent)

/// ribonuclease L (2',5'- oligoisoadenylate synthetase-dependent)/// ribonuclease L (2 ', 5'-oligoisoadenylate synthetase-dependent)

890 225990 at BOC brother of CDO W72626 Hs.153961.2 NP. 150279 Chr:3q13.2 0.40844 1.380569890 225990 at BOC brother of CDO W72626 Hs.153961.2 NP . 150279 Chr: 3q13.2 0.40844 1.380569

891 239113 at — Transcribed sequences AV749665 Hs.130531.0 — — 0.40826 0.353669891 239113 at - Transcribed sequences AV749665 Hs.130531.0 - - 0.40826 0.353669

892 203799. _at DCL-1 type I transmembrane NM_014880 g7661867 NP. J355695 Chr:2q24.2 0.40795 0.095261 C-type lectin receptor DCL-1892 203799. _at DCL-1 type I transmembrane NM_014880 g7661867 NP. J355695 Chr: 2q24.2 0.40795 0.095261 C-type lectin receptor DCL-1

893 207869. _s_at CACNA1G calcium Channel, NM_018896 g9256522 NP. .061496 Chr:17q22 0.40751 0.149911 voltage-dependent, alpha IG subunit893 207869. _s_at CACNA1G Calcium Channel, NM_018896 g9256522 NP. .061496 Chr: 17q22 0.40751 0.149911 voltage-dependent, alpha IG subunit

894 202732 at PKIG protein kinase (cAMP- NM_007066 . g5902019 NP_008997 Chr:20q12- 0.40694 0.095261 dependent, catalytic) q13.1 inhibitor gamma /// protein kinase (cAMP- dependent, catalytic) inhibitor gamma894 202732 at PKIG protein kinase (cAMP-NM_007066.g5902019 NP_008997 Chr: 20q12- 0.40694 0.095261 dependent, catalytic) q13.1 inhibitor gamma /// protein kinase (cAMP-dependent, catalytic) inhibitor gamma

895 229661. jat SALL4 sal-like 4 (Drosophila) NM. „020436 Hs.189095.0 NP. .065169 Chr:20q13.13- 0.40678 0.625663 q13.2895 229661. jat SALL4 sal-like 4 (Drosophila) NM. " 020436 Hs.189095.0 NP . .065169 Chr: 20q13.13- 0.40678 0.625663 q13.2

896 201236. _s_at BTG2 BTG family, member 2 NM. .006763 g5802987 NP. J306754 Chr:1q32 0.40402 0.625663 /// BTG family, member 2 896 201236._s_at BTG2 BTG family, member 2 NM . .006763 g5802987 NP. J306754 Chr: 1q32 0.40402 0.625663 /// BTG family, member 2

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

897 207391 s at PIP5K1A phosphatidylinositol-4- NM_003557 g4505814 NP_003548 Chr:1q22-q24 0.4039 2.571322 phosphate 5-kinase, type I, alpha /// phosphatidylinositol-4- phosphate 5-kinase, type I, alpha897 207391 s at PIP5K1A phosphatidylinositol-4- NM_003557 g4505814 NP_003548 Chr: 1q22-q24 0.4039 2.571322 phosphates 5-kinase, type I, alpha /// phosphatidylinositol-4-phosphate 5-kinase, type I, alpha

898 204134 at PDE2A Phosphodiesterase 2A, NM_002599 g4505656 NP_002590 Chr:11q13.3 0.40378 0.095261 cGMP-stimulated /// Phosphodiesterase 2A, cGMP-stimulated898 204134 at PDE2A phosphodiesterase 2A, NM_002599 g4505656 NP_002590 Chr: 11q13.3 0.40378 0.095261 cGMP-stimulated /// phosphodiesterase 2A, cGMP-stimulated

899 236016 at SMARCE1 SWI/SNF reiated, AI702962 Hs.127211.0 NP_003070 Chr:17q21.2 0.40366 0.095261 matrix associated, actin dependent regulator of chromatin, subfamily e, member 1899 236016 at SMARCE1 SWI / SNF reiated, AI702962 Hs.127211.0 NP_003070 Chr: 17q21.2 0.40366 0.095261 matrix associated, actin dependent regulator of chromatin, subfamily e, member 1

900 227478_ _at BF739885 Hs.128052.0 0.40337 0.149911900 227478_ _at BF739885 Hs.128052.0 0.40337 0.149911

901 219948. _x_at FLJ21934 hypothetical protein NM_024743 gl 3376065 NP. _079019 Chr:4q13.3 0.40312 1.380569 FLJ21934901 219948. _x_at FLJ21934 hypothetical protein NM_024743 gl 3376065 NP. _079019 Chr: 4q13.3 0.40312 1.380569 FLJ21934

902 225557. _at Hs.6607.0 0.40255 0.095261902 225557 . _at Hs.6607.0 0.40255 0.095261

903 201061. s_at STOM stomatin M81635 g181183 NP. _004090 Chr:9q34.1 0.40207 0.095261903 201061. s_at STOM stomatin M81635 g181183 NP . _004090 Chr: 9q34.1 0.40207 0.095261

904 230786 at Transcribed sequence AI424786 Hs.99298.0 0.40173 0.353669 with moderate similarity to protein sp:P39188904 230786 at Transcribed sequence AI424786 Hs.99298.0 0.40173 0.353669 with moderate similarity to protein sp: P39188

(H.sapiens)(H.sapiens)

ALU1_HUMAN AIu subfamily J sequence contamination warning entryALU1_HUMAN AI subfamily J sequence contamination warning entry

905 230624_ .at SLC25A27 solute carrier family 25, AW779950 Hs.145008.0 NP_004268 Chr:6p11.2-q12 0.40159 9.266777 member 27905 230624_ .at SLC25A27 solute carrier family 25, AW779950 Hs.145008.0 NP_004268 Chr: 6p11.2-q12 0.40159 9.266777 member 27

906 203129_ _s_at KIF5C kinesin family member BF059313 Hs.6641.0 Chr:2q23.3 0.40149 1.380569 5C906 203129_ _s_at KIF5C kinesin family member BF059313 Hs.6641.0 Chr: 2q23.3 0.40149 1.380569 5C

907 209612„ _s_at ADH1 B alcohol dehydrogenase M24317 gl 78097 NP_000659 Chr:4q21-q23 0.40139 8.153992 IB (class I), beta Polypeptide 907 209612 " _s_at ADH1 B alcohol dehydrogenase M24317 gl 78097 NP_000659 Chr: 4q21-q23 0.40139 8.153992 IB (class I), beta polypeptides

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

908 236235_at ITCH itchy homolog E3 AA868238 Hs.128713.0 NP_113671 Chr:20q11.22- 0.40115 0.779487 ubiquitin protein ligase q11.23 (mouse)908 236235_at ITCH itchy homologue E3 AA868238 Hs.128713.0 NP_113671 Chr: 20q11.22- 0.40115 0.779487 ubiquitin protein ligase q11.23 (mouse)

909 216594_x_at Hs.306098.1 0.40108 0.149911909 216594_x_at Hs.306098.1 0.40108 0.149911

910 213872_at C6orf62 chromosome 6 open BE465032 Hs.173685.1 NPJ 12201 Chr:6p22.1 0.40089 0.625663 reading frame 62910 213872_at C6orf62 chromosomes 6 open BE465032 Hs.173685.1 NPJ 12201 Chr: 6p22.1 0.40089 0.625663 reading frame 62

911 214416_at — — AA654415 Hs.322808.0 — — 0.40067 3.612633911 214416_at - - AA654415 Hs.322808.0 - - 0.40067 3.612633

912 236222 at AAT1 AAT1 -alpha N62817 Hs.48643.0 NP 203528 Chr:3q12-q13.3 0.40051 6.023405912 236222 at AAT1 AAT1 -alpha N62817 Hs.48643.0 NP 203528 Chr: 3q12-q13.3 0.40051 6.023405

913 230867 at AI742521 Hs.120388.0 0.40046 1.380569913 230867 at AI742521 Hs.120388.0 0.40046 1.380569

914 244455_at SLICK sodium- and chloride- AI732637 Hs.277901.0 NP_940905 Chr:1q31.2 0.40029 0.518167 activated ATP-sensitive potassium Channel914 244455_at SLICK sodium- and chloride- AI732637 Hs.277901.0 NP_940905 Chr: 1q31.2 0.40029 0.518167 activated ATP-sensitive potassium Channel

915 201842_s_at EFEMP1 EGF-containing fibulin- AI826799 Hs.76224.0 NPJD04096 Chr:2p16 0.40016 0.149911 like extracellular matrix protein 1915 201842_s_at EFEMP1 EGF-containing fibulin AI826799 Hs.76224.0 NPJD04096 Chr: 2p16 0.40016 0.149911 like extracellular matrix protein 1

916 240253 at — Transcribed sequences BF508634 Hs.210775.0 — — 0.39999 11.55252916 240253 at - Transcribed sequences BF508634 Hs.210775.0 - - 0.39999 11.55252

917 209170_s_at GPM6B glycoprotein M6B AF016004 g3387766 NP 005269 Chr:xp22.2 0.39961 4.174189917 209170_s_at GPM6B glycoprotein M6B AF016004 g3387766 NP 005269 Chr: xp22.2 0.39961 4.174189

918 229228_at — CDNA FLJ32589 fis, AI819043 Hs.21342.0 — — 0.39931 0.149911 clone SPLEN2000443918 229228_at - CDNA FLJ32589 fis, AI819043 Hs.21342.0 - - 0.39931 0.149911 clone SPLEN2000443

919 225207_at PDK4 pyruvate AV707102 Hs.8364.0 NPJD02603 Chr:7q21.3- 0.39816 11.55252 dehydrogenase kinase, q22.1 isoenzyme 4919 225207_at PDK4 pyruvate AV707102 Hs.8364.0 NPJD02603 Chr: 7q21.3- 0.39816 11.55252 dehydrogenase kinase, q22.1 isoenzymes 4

920 217525_at UNQ564 MVAL564 AW305097 Hs.47974.0 NP 940876 Chr:11p15.4 0.39735 0.201325920 217525_at UNQ564 MVAL564 AW305097 Hs.47974.0 NP 940876 Chr: 11p15.4 0.39735 0.201325

921 219832_s_at HOXC13 homeo box C13 /// NM_017410 g8393553 NP_059106 Chr:12q13.3 0.39708 0.095261 homeo box C13921 219832_s_at HOXC13 homeo box C13 /// NM_017410 g8393553 NP_059106 Chr: 12q13.3 0.39708 0.095261 homeo box C13

922 218312_s_at FLJ 12895 hypothetical protein NMJ323926 gl 2965194 NP_076415 Chr:19q13.43 0.39693 8.779357 FLJ12895922 218312_s_at FLJ 12895 hypothetical protein NMJ323926 gl 2965194 NP_076415 Chr: 19q13.43 0.39693 8.779357 FLJ12895

923 232855_at MRNA füll length insert AL360204 Hs.283853.0 0.39647 0.095261 cDNA clone EUROIMAGE 980547923 232855_at MRNA filling length insert AL360204 Hs.283853.0 0.39647 0.095261 cDNA clone EUROIMAGE 980547

924 239911_at — Transcribed sequences H49805 Hs.246005.0 — — 0.39607 1.051298924 239911_at - Transcribed sequences H49805 Hs.246005.0 - - 0.39607 1.051298

925 214844_s_at Hs.78006.0 0.39516 6.991358925 214844_s_at Hs.78006.0 0.39516 6.991358

926 244592_at — Transcribed sequences BE675040 Hs.208603.0 — — 0.39403 1.051298926 244592_at - Transcribed sequences BE675040 Hs.208603.0 - - 0.39403 1.051298

927 205407_at NM_021111 gl 1863155 0.39347 1.051298 927 205407_at NM_021111 gl 1863155 0.39347 1.051298

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-vaiue Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-vaiue Sample Number ID Protein ID Localization Change (%)

928 228370_at SNRPN small nuclear BF114870 Hs.58606.1 NP_003088 Chr:15q12 0.39335 0.095261 ribonucleoprotein Polypeptide N928 228370_at SNRPN small nuclear BF114870 Hs.58606.1 NP_003088 Chr: 15q12 0.39335 0.095261 ribonucleoprotein polypeptides N

929 206002_at GPR64 G protein-coupled NMJ)05756 g5031732 NP_005747 Chr:xp22.22 0.39296 0.353669 receptor 64 /// G protein-coupled receptor 64929 206002_at GPR64 G protein-coupled NMJ) 05756 g5031732 NP_005747 Chr: xp22.22 0.39296 0.353669 receptor 64 /// G protein-coupled receptor 64

930 211590_x_at TBXA2R thromboxaπe A2 U11271 g511793 NPJ)OI 051 Chr:19p13.3 0.39263 0.095261 receptor930 211590_x_at TBXA2R thromboxane A2 U11271 g511793 NPJ) OI 051 Chr: 19p13.3 0.39263 0.095261 receptor

931 225987 at AA650281 Hs.11177.0 0.39224 0.095261931 225987 at AA650281 Hs.11177.0 0.39224 0.095261

932 212230_at PPAP2B phosphatidic acid AV725664 Hs.173717.0 NP_003704 Chr:1pter-p22.1 0.39217 0.095261 Phosphatase type 2B932 212230_at PPAP2B phosphatidic acid AV725664 Hs.173717.0 NP_003704 Chr: 1pter-p22.1 0.39217 0.095261 Phosphatase type 2B

933 235438_at AW162011 Hs.146226.0 0.39152 0.353669933 235438_at AW162011 Hs.146226.0 0.39152 0.353669

934 210198 s at PLP1 proteolipid protein 1 BC002665 gl 2803660 NP_000524 Chr:xq22 0.39085 2.814919 (Pelizaeus-Merzbacher disease, spastic paraplegia 2, uncomplicated) /// proteolipid protein 1 (Pelizaeus-Merzbacher disease, spastic paraplegia 2, uncomplicated)934 210198 s at PLP1 proteolipid protein 1 BC002665 gl 2803660 NP_000524 Chr: xq22 0.39085 2.814919 (Pelizaeus-Merzbacher disease, spastic paraplegia 2, uncomplicated) /// proteolipid protein 1 (Pelizaeus-Merzbacher disease, spastic paraplegia 2, uncomplicated)

935 227762 at ZNF145 zinc finger protein 145 AW244016 Hs.22971.0 NP_005997 Chr:11q23.1 0.39014 0.779487 (Kruppel-like, expressed in promyelocytic leukemia)935 227762 at ZNF145 zinc finger protein 145 AW244016 Hs.22971.0 NP_005997 Chr: 11q23.1 0.39014 0.779487 (Kruppel-like, expressed in promyelocytic leukemia)

936 230271. _at — CDNA FLJ42567 fis, BG150301 Hs.127039.0 — — 0.38948 0.095261 clone BRACE3007559936 230271. _at - CDNA FLJ42567 fis, BG150301 Hs.127039.0 - - 0.38948 0.095261 clone BRACE3007559

937 206406. _at MCSP mitochondrial capsule NM_030663 gl 3489057 NP_109588 Chr:1q21.3 0.38937 0.095261 selenoprotein /// mitochondrial capsule

Figure imgf000072_0001
937 206406. _at MCSP mitochondrial capsule NM_030663 gl 3489057 NP_109588 Chr: 1q21.3 0.38937 0.095261 selenoprotein /// mitochondrial capsule
Figure imgf000072_0001

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer IP Protein ID Lokalisierung ChangeSample Number IP Protein ID Localization Change

938 206410 at NR0B2 nuclear receptor NM_021969 gl 3259502 NP_068804 Chr:1p36.1 0.38927 8.153992 sυbfamily 0, group B, member 2 /// nuclear receptor subfamily 0, group B, member 2938 206410 at NR0B2 nuclear receptor NM_021969 gl 3259502 NP_068804 Chr: 1p36.1 0.38927 8.153992 sυbfamily 0, group B, member 2 /// nuclear receptor subfamily 0, group B, member 2

939 224215 s at DLL1 delta-like 1 AF196571 g6180181 NP_005609 Chr:6q27 0.38906 1.051298 (Drosophila)939 224215 s at DLL1 delta-like 1 AF196571 g6180181 NP_005609 Chr: 6q27 0.38906 1.051298 (Drosophila)

940 203543 s at BTEB1 basic transcriptioπ NM_001206 g4557374 NP_001197 Chr:9q13 0.38896 0.518167 element binding protein 1 /// basic transcription element binding protein 1940 203543 s at BTEB1 basic transcriptioπ NM_001206 g4557374 NP_001197 Chr: 9q13 0.38896 0.518167 element binding protein 1 /// basic transcription element binding protein 1

941 218532. _s_at FLJ20152 hypothetical protein NM_019000 g9506660 NP. .061873 Chr:5p15.1 0.3885 0.095261 FLJ20152941 218532. _s_at FLJ20152 hypothetical protein NM_019000 g9506660 NP . .061873 Chr: 5p15.1 0.3885 0.095261 FLJ20152

942 232267 _at Hs.158258.0 0.38839 0.518167942 232267 _at Hs.158258.0 0.38839 0.518167

943 237628. _at — Transcribed sequences BF196209 Hs.245842.0 — — 0.38831 0.095261943 237628. _at - Transcribed sequences BF196209 Hs.245842.0 - - 0.38831 0.095261

944 235561 _at — Transcribed sequences T16544 Hs.291005.0 — — 0.38822 9.266777944 235561 _at - Transcribed sequences T16544 Hs.291005.0 - - 0.38822 9.266777

945 222071. _s_at SLCO4C1 solute carrier organic BE552428 Hs.127648.1 NP. .060985 Chr:5q21.2 0.38795 4.436744 κ1 anion transporter family, member 4C1945 222071. _s_at SLCO4C1 solute carrier organic BE552428 Hs.127648.1 NP. , 060985 Chr: 5q21.2 0.38795 4.436744 κ1 anion transporter family, member 4C1

946 238747_ _at Clone R45692 Hs.90242.0 0.3876 0.095261 IMAGE:4796172, mRNA946 238747_ _at Clone R45692 Hs.90242.0 0.3876 0.095261 IMAGE: 4796172, mRNA

947 238329. _at M-RIP myosin phosphatase- BE545235 Hs.159336.0 NP. .055949 Chr:17p11.2 0.38675 0.095261 Rho interacting protein947 238329. _at M-RIP myosin phosphatase BE545235 Hs.159336.0 NP. .055949 Chr: 17p11.2 0.38675 0.095261 Rho interacting protein

948 205609. _at ANGPT1 angiopoietin 1 NM 001146 g4502086 NP 001137 Chr:8q22.3-q23 0.38673 0.095261948 205609 . _at ANGPT1 angiopoietin 1 NM 001146 g4502086 NP 001137 Chr: 8q22.3-q23 0.38673 0.095261

949 208853. _s_at CANX calnexin L18887 g306480 NP. .001737 Chr:5q35 0.38477 0.095261949 208853. _s_at CANX calnexin L18887 g306480 NP. .001737 Chr: 5q35 0.38477 0.095261

950 237400 at ATP5S ATP synthase, H+ AI308101 Hs.22978.0 NP_056499 Chr:14q22.1 0.38473 0.149911 transporting, mitochondrial FO complex, subunit s (factor B)950 237400 ATP5S ATP synthase, H + AI308101 Hs.22978.0 NP_056499 Chr: 14q22.1 0.38473 0.149911 transporting, mitochondrial FO complex, subunit s (factor B)

951 211479 s at HTR2C 5-hydroxytryptamine M81778 g338027 NP_000859 Chr:xq24 0.38471 5.685182 (Serotonin) receptor 2C /// 5-hydroxytryptamine (Serotonin) receptor 2C 951 211479 s at HTR2C 5-hydroxytryptamine M81778 g338027 NP_000859 Chr: xq24 0.38471 5.685182 (serotonin) receptor 2C /// 5-hydroxytryptamine (serotonin) receptor 2C

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung ChangeNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change

952 210299_s_at FHL1 four and a half LIM AF063002 g3859848 NP_001440 Chr:xq26 0.38428 1.380569 domains 1952 210299_s_at FHL1 four and a half LIM AF063002 g3859848 NP_001440 Chr: xq26 0.38428 1.380569 domains 1

953 212372_at MYH10 myosin, heavy AK026977 Hs.296842.0 NP_005955 Chr:17p13 0.38427 1.051298 Polypeptide 10, non- muscle953 212372_at MYH10 myosin, heavy AK026977 Hs.296842.0 NP_005955 Chr: 17p13 0.38427 1.051298 Polypeptides 10, non-muscle

954 222885_at EMCN endomucin AF205940 g8547214 NP 057326 Chr:4q23 0.38355 0.095261954 222885_at EMCN endomucin AF205940 g8547214 NP 057326 Chr: 4q23 0.38355 0.095261

955 202054 s at ALDH3A2 aldehyde NM_000382 g4557302 NP_000373 Chr:17p11.2 0.38315 0.095261 dehydrogenase 3 family, member A2 /// aldehyde dehydrogenase 3 family, member A2955 202054 s at ALDH3A2 aldehydes NM_000382 g4557302 NP_000373 Chr: 17p11.2 0.38315 0.095261 dehydrogenase 3 family, member A2 /// aldehyde dehydrogenase 3 family, member A2

956 205979_at SCGB2A1 secretoglobin, family NM_002407 g4505170 NP_002398 Chr:11q13 0.38241 1.901012 2A, member 1 /// secretoglobin, family 2A, member 1956 205979_at SCGB2A1 secretoglobin, family NM_002407 g4505170 NP_002398 Chr: 11q13 0.38241 1.901012 2A, member 1 /// secretoglobin, family 2A, member 1

957 227497_at CDNA FLJ 11723 fis, BE326808 Hs.196008.0 0.38213 11.55252 clone HEMBA1005314 -4957 227497_at CDNA FLJ 11723 fis, BE326808 Hs.196008.0 0.38213 11.55252 clone HEMBA1005314 -4

958 221541 at gl 2053226 0.38167 0.149911958 221541 at gl 2053226 0.38167 0.149911

959 221031_s_at DKFZP434F0318 hypothetical protein NM_030817 gl 3540611 NPJ 10444 Chr:12p13.2 0.38133 0.095261 DKFZp434F0318 /// hypothetical protein DKFZp434F0318959 221031_s_at DKFZP434F0318 hypothetical protein NM_030817 gl 3540611 NPJ 10444 Chr: 12p13.2 0.38133 0.095261 DKFZp434F0318 /// hypothetical protein DKFZp434F0318

960 204621_s_at NR4A2 nuclear receptor AI935096 Hs.82120.0 NP_006177 Chr:2q22-q23 0.38005 0.518167 subfamily 4, group A, member 2960 204621_s_at NR4A2 nuclear receptor AI935096 Hs.82120.0 NP_006177 Chr: 2q22-q23 0.38005 0.518167 subfamily 4, group A, member 2

961 241505 at — BF513468 Hs.196779.0 — — 0.37953 0.353669961 241505 at - BF513468 Hs.196779.0 - - 0.37953 0.353669

962 32502_at PP1665 hypothetical protein AL041124 4922580_RC NPJ 10419 Chr:11q13.3 0.37813 0.095261 PP1665962 32502_at PP1665 hypothetical protein AL041124 4922580_RC NPJ 10419 Chr: 11q13.3 0.37813 0.095261 PP1665

963 216675_at — AK000834 Hs.306409.0 — — 0.37809 0.095261963 216675_at - AK000834 Hs306409.0 - - 0.37809 0.095261

964 204115 at GNG11 guanine nucleotide NM_004126 g4758447 NP_004117 Chr:7q31-q32 0.37604 0.095261 binding protein (G protein), gamma 11 /// guanine nucleotide binding protein (G protein), gamma 11 964 204115 at GNG11 guanine nucleotide NM_004126 g4758447 NP_004117 Chr: 7q31-q32 0.37604 0.095261 binding protein (G protein), gamma 11 /// guanine nucleotide binding protein (G protein), gamma 11

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

965 204151_x_at g5453542 0.37405 0.201325965 204151_x_at g5453542 0.37405 0.201325

966 39248_at AQP3 aquaporin 3 N74607 4855867 RC NP 004916 Chr:9p13 0.37169 0.095261966 39248_at AQP3 aquaporin 3 N74607 4855867 RC NP 004916 Chr: 9p13 0.37169 0.095261

967 239349_at BE856929 Hs.50636.0 0.37048 0.095261967 239349_at BE856929 Hs.50636.0 0.37048 0.095261

968 238919 at R49295 Hs.24886.0 0.37036 0.353669968 238919 at R49295 Hs.24886.0 0.37036 0.353669

969 238481_at MGP matrix GIa protein AW512787 Hs.313944.0 NP_000891 Chr:12p13.1- 0.3699 4.640066 P12.3969 238481_at MGP matrix GIa protein AW512787 Hs.313944.0 NP_000891 Chr: 12p13.1- 0.3699 4.640066 P12.3

970 241302 at — Traπscribed sequences AI654048 Hs.196556.0 — — 0.36984 1.702877970 241302 at - Traπscribed sequences AI654048 Hs.196556.0 - - 0.36984 1.702877

971 221463_at CCL24 chemokine (C-C motif) NMJD02991 g4506838 NP_002982 Chr:7q11.23 0.36944 1.702877 ligaπd 24 /// chemokine (C-C motif) ligand 24971 221463_at CCL24 chemokines (C-C motif) NMJD02991 g4506838 NP_002982 Chr: 7q11.23 0.36944 1.702877 ligaπd 24 /// chemokines (C-C motif) ligand 24

972 219440_at RAI2 retinoic acid induced 2 NM_021785 gl 2056469 NP_068557 Chr:xp22 0.36869 0.095261 /// retinoic acid induced 2972 219440_at RAI2 retinoic acid induced 2 NM_021785 gl 2056469 NP_068557 Chr: xp22 0.36869 0.095261 /// retinoic acid induced 2

973 216728_at Hs.306520.0 0.36825 0.095261973 216728_at Hs.306520.0 0.36825 0.095261

974 217533 x at Transcribed sequence AW268081 Hs.193408.0 0.36792 0.149911 with moderate similarity to protein -4974 217533 x at transcribed sequence AW268081 Hs.193408.0 0.36792 0.149911 with moderate similarity to protein -4

4- ref:NP_060312.14- ref: NP_060312.1

(H.sapiens) hypothetical protein(H.sapiens) hypothetical protein

FLJ20489 [Homo sapiens]FLJ20489 [Homo sapiens]

975 220971_at IL17E interleukin 17E /// NM_022789 gl 2232484 NP. _073626 Chr:14q11.2 0.36763 0.095261 interleukin 17E975 220971_at IL17E interleukin 17E /// NM_022789 gl 2232484 NP . _073626 Chr: 14q11.2 0.36763 0.095261 interleukin 17E

976 231511_at FRAS1 Fräser Syndrome 1 AI681772 Hs.15420.0 NP 065926 Chr:4q21.21 0.36693 0.095261976 231511_at FRAS1 Router Syndrome 1 AI681772 Hs.15420.0 NP 065926 Chr: 4q21.21 0.36693 0.095261

977 235774_at — CDNA FLJ42409 fis, AV699047 Hs.94946.0 — — 0.3668 9.266777 clone BLADE2000866977 235774_at - CDNA FLJ42409 fis, AV699047 Hs.94946.0 - - 0.3668 9.266777 clone BLADE2000866

978 209687_at CXCL12 chemokine (C-X-C U 19495 gl 754834 NP. _000600 Chr:10q11.1 0.36648 11.06357 motif) ligand 12 (stromal cell-derived factor 1 )978 209687_at CXCL12 chemokines (C-X-C U 19495 gl 754834 NP. _000600 Chr: 10q11.1 0.36648 11.06357 motif) ligand 12 (stromal cell-derived factor 1)

979 214761_at ZNF423 zinc finger protein 423 AW149417 Hs.137168.0 NP. 055884 Chr:16q12 0.36637 3.62324979 214761_at ZNF423 zinc finger protein 423 AW149417 Hs.137168.0 NP . 055884 Chr: 16q12 0.36637 3.62324

980 205907_s_at OMD osteomodulin AI765819 Hs.94070.0 NP. 005005 Chr:9q22.32 0.36637 3.62324 980 205907_s_at OMD osteomodulin AI765819 Hs.94070.0 NP . 005005 Chr: 9q22.32 0.36637 3.62324

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

981 209815_at CDNA FLJ42602 fis, BG054916 Hs.159526.0 0.3656 0.353669 clone BRACE3011271 , moderately similar to Patched protein981 209815_at CDNA FLJ42602 fis, BG054916 Hs.159526.0 0.3656 0.353669 clone BRACE3011271, moderately similar to Patched protein

982 204955_at SRPX sυshi-repeat-containing NM_006307 g5454085 NP_006298 Chr:xp21.1 0.36484 0.353669 protein, X-linked /// sushi-repeat-containing protein, X-linked982 204955_at SRPX sυshi-repeat-containing NM_006307 g5454085 NP_006298 Chr: xp21.1 0.36484 0.353669 protein, X-linked /// sushi-repeat-containing protein, X-linked

983 232593_at LOC93082 hypothetical protein AL389981 Hs.149219.0 NP_612406 Chr:2q11.2 0.36414 1.380569 BC012317Hypothetical protein AL389981 Hs.149219.0 NP_612406 Chr: 2q11.2 0.36414 1.380569 BC012317

984 207859 s at CHRNB3 cholinergic receptor, NM_000749 g4502834 NP_000740 Chr:8p11.2 0.36377 3.612633 nicotinic, beta Polypeptide 3 /// cholinergic receptor, nicotinic, beta Polypeptide 3984 207859 s at CHRNB3 cholinergic receptor, NM_000749 g4502834 NP_000740 Chr: 8p11.2 0.36377 3.612633 nicotinic, beta polypeptides 3 /// cholinergic receptor, nicotinic, beta polypeptides 3

985 203016_s_at SSX2IP synovial sarcoma, X AK001710 Hs.22587.0 NP. _054740 Chr:1p22.3 0.36292 13.40329 breakpoint 2 interacting -4 protein985 203016_s_at SSX2IP synovial sarcoma, X AK001710 Hs.22587.0 NP . _054740 Chr: 1p22.3 0.36292 13.40329 breakpoint 2 interacting -4 protein

986 239657_x_at . FOXO6 forkhead box protein AI341823 Hs.133066.0 — Chr:1 p34.1 0.3626 0.353669 06986 239657_x_at. FOXO6 forkhead box protein AI341823 Hs.133066.0 - Chr: 1 p34.1 0.3626 0.353669 06

987 210751_s_at RGN regucalcin (senescence D31815 gl 072311 NP_ .004674 Chr:xp11.3 0.3626 0.095261 marker protein-30) /// regucalcin (senescence marker protein-30)987 210751_s_at RGN regucalcin (senescence D31815 gl 072311 NP_ .004674 Chr: xp11.3 0.3626 0.095261 marker protein-30) /// regucalcin (senescence marker protein-30)

988 203498_at DSCR1L1 Down Syndrome critical NM_005822 g5032234 NP_ .005813 Chr:6p21.1 0.3626 8.779357 region gene 1-like 1 /// Down Syndrome critical region gene 1-like 1988 203498_at DSCR1L1 Down Syndrome critical NM_005822 g5032234 NP_ .005813 Chr: 6p21.1 0.3626 8.779357 region gene 1-like 1 /// Down Syndrome critical region gene 1-like 1

989 230560 at N21096 Hs.22588.0 0.36177 6.023405989 230560 at N21096 Hs.22588.0 0.36177 6.023405

990 228694_at Clone BE670036 Hs.16886.0 0.36177 0.095261 IMAGE:3352913, mRNA990 228694_at Clone BE670036 Hs.16886.0 0.36177 0.095261 IMAGE: 3352913, mRNA

991 211143_x_at NR4A1 nuclear receptor D49728 gl 813881 NP_ _002126 Chr:12q13 0.36113 0.518167 subfamily 4, group A, member 1 991 211143_x_at NR4A1 nuclear receptor D49728 gl 813881 NP_ _002126 Chr: 12q13 0.36113 0.518167 subfamily 4, group A, member 1

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

992 228692_at — CDNA FLJ41396 fis, N63377 Hs.169943.0 — — 0.36084 0.201325 clone BRCOC2019255992 228692_at - CDNA FLJ41396 fis, N63377 Hs.169943.0 - - 0.36084 0.201325 clone BRCOC2019255

993 219230_at FLJ10970 hypothetical protein NM_018286 g8922795 NP_060756 Chr:17q23.1 0.36058 3.612633 FLJ10970993 219230_at FLJ10970 hypothetical protein NM_018286 g8922795 NP_060756 Chr: 17q23.1 0.36058 3.612633 FLJ10970

994 202173_s_at ZNF161 zinc finger protein 161 NM_007146 g6005967 NP 009077 Chr:17q23.2 0.36026 0.625663994 202173_s_at ZNF161 zinc finger protein 161 NM_007146 g6005967 NP 009077 Chr: 17q23.2 0.36026 0.625663

995 226495_at KIAA1271 KIAA1271 protein BE727883 Hs.183669.0 NP 065797 Chr:20p13 0.36014 0.095261995 226495_at KIAA1271 KIAA1271 protein BE727883 Hs.183669.0 NP 065797 Chr: 20p13 0.36014 0.095261

996206726 at PGDS Prostaglandin D2 NMJD14485 g7657456 NP_055300 Chr:4q22.3 0.36012 11.70204 synthase, hematopoietic /// Prostaglandin D2 synthase, hematopoietic996206726 at PGDS prostaglandin D2 NMJD14485 g7657456 NP_055300 Chr: 4q22.3 0.36012 11.70204 synthase, hematopoietic /// prostaglandin D2 synthase, hematopoietic

997 236277 at H23551 Hs.30974.0 0.36011 0.353669997 236277 at H23551 Hs.30974.0 0.36011 0.353669

998 204560_at FKBP5 FK506 binding protein NM_004117 g4758383 NP_004108 Chr:6p21.3-21.2 0.35973 0.095261 5 /// FK506 binding protein 5998 204560_at FKBP5 FK506 binding protein NM_004117 g4758383 NP_004108 Chr: 6p21.3-21.2 0.35973 0.095261 5 /// FK506 binding protein 5

999 238527_at CDNA clone BG106878 Hs.204724.0 0.35962 0.095261 -4 IMAGE:4779711 , partial cds999 238527_at CDNA clone BG106878 Hs.204724.0 0.35962 0.095261 -4 IMAGE: 4779711, partial cds

1000 209699_x_at g531159 0.35825 0.3536691000 209699_x_at g531159 0.35825 0.353669

1001 224355 s at MS4A8B membrane-spanning 4- AF237905 g13649389 NP_113645 Chr:11q12.2 0.35802 0.201325 domains, subfamily A, member 8B ///1001 224355 s at MS4A8B membrane spanning 4- AF237905 g13649389 NP_113645 Chr: 11q12.2 0.35802 0.201325 domains, subfamily A, member 8B ///

3 13 1

Figure imgf000077_0001
Figure imgf000077_0001

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

1004 206336 at CXCL6 chemokine (C-X-C NM_002993 g4506850 NP_002984 Chr:4q21 0.35674 0.625663 motif) ligand 6 (granulocyte chemotactic protein 2) /// chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2)1004 206336 at CXCL6 chemokines (C-X-C NM_002993 g4506850 NP_002984 Chr: 4q21 0.35674 0.625663 motif) ligand 6 (granulocyte chemotactic protein 2) /// chemokines (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2)

1005 228724_at Clone N49237 Hs.22542.0 0.35659 6.023405 IMAGE-.5312516, mRNA1005 228724_at Clone N49237 Hs.22542.0 0.35659 6.023405 IMAGE-.5312516, mRNA

1006 227758_at RERG RAS-like, estrogen- AW294092 Hs.21594.0 NP_116307 Chr:12p13.1 0.35548 0.095261 regulated, growth inhibitor1006 227758_at RERG RAS-like, estrogen- AW294092 Hs.21594.0 NP_116307 Chr: 12p13.1 0.35548 0.095261 regulated, growth inhibitor

1007 205498_at GHR growth hormone NM_000163 g4503992 NP_000154 Chr:5p13-p12 0.3554 0.353669 receptor /// growth hormone receptor1007 205498_at GHR growth hormone NM_000163 g4503992 NP_000154 Chr: 5p13-p12 0.3554 0.353669 receptor /// growth hormone receptor

1008 242342_at — CDNA FLJ40823 fis, BE645219 Hs.134863.0 — — 0.35536 0.095261 clone TRACH20110931008 242342_at - CDNA FLJ40823 fis, BE645219 Hs.134863.0 - - 0.35536 0.095261 clone TRACH2011093

1009 229465_s_at PTPRS protein tyrosine BF433071 Hs.294141.1 NP_002841 Chr:19p13.3 0.35433 4.75532 Phosphatase, receptor type, S1009 229465_s_at PTPRS protein tyrosine BF433071 Hs.294141.1 NP_002841 Chr: 19p13.3 0.35433 4.75532 Phosphatase, receptor type, S

1010 203881 s at DMD dystrophin (muscular NM_004010 g5032314 NP_000100 Chr:xp21.2 0.35415 0.149911 dystrophy, Duchenne and Becker types) /// dystrophin (muscular dystrophy, Duchenne and Becker types)1010 203881 s at DMD dystrophin (muscular NM_004010 g5032314 NP_000100 Chr: xp21.2 0.35415 0.149911 dystrophy, Duchenne and Becker types) /// dystrophin (muscular dystrophy, Duchenne and Becker types)

1011 229199 at Transcribed sequence BE856807 Hs.13132.0 0.3537 0.353669 with weak similarity to protein sp:P39188 (H.sapiens) ALUIJHUMAN AIU subfamily J sequence contamination waming entry 1011 229199 at Transcribed sequence BE856807 Hs.13132.0 0.3537 0.353669 with weak similarity to protein sp: P39188 (H.sapiens) ALUIJHUMAN AIU subfamily J sequence contamination waming entry

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change /0/ Sample ID ID Protein ID Localization Change / 0 /

1012 221293 s at DEF6 differentially expressed NMJ022047 gl 1545748 NP_071330 Chr:6p21.33- 0.35342 0.095261 in FDCP 6 homolog p21.1 (mouse) /// differentially expressed in FDCP 6 homolog (mouse)1012 221293 s at DEF6 differentially expressed NMJ022047 gl 1545748 NP_071330 Chr: 6p21.33- 0.35342 0.095261 in FDCP 6 homologous p21.1 (mouse) /// differentially expressed in FDCP 6 homologous (mouse)

1013 215237, _at DOCK9 dedicator of cytokinesis AW450751 Hs.8021.1 NP. J056111 Chr:13q32.3 0.35316 0 .149911 91013 215237 , _at DOCK9 dedicator of cytokinesis AW450751 Hs.8021.1 NP. J056111 Chr: 13q32.3 0.35316 0 .149911 9

1014 227182, _at SUSD3 sushi domain AW966474 Hs.88417.0 NP. _659443 Chr:9q22.32 0.35279 1 .051298 containing 31014 227182 , _at SUSD3 sushi domain AW966474 Hs.88417.0 NP . _659443 Chr: 9q22.32 0.35279 1 .051298 containing 3

1015 207210 at GABRA3 gamma-aminobutyric NM_000808 g4557602 NP_000799 Chr:xq28 0.35258 0.149911 acid (GABA) A receptor, alpha 3 /// gamma-am inobutyric acid (GABA) A receptor, alpha 31015 207210 at GABRA3 gamma-aminobutyric NM_000808 g4557602 NP_000799 Chr: xq28 0.35258 0.149911 acid (GABA) A receptor, alpha 3 /// gamma-aminobutyric acid (GABA) A receptor, alpha 3

1016 213156_at Hs.16193.0 0.35249 0.5181671016 213156_at Hs.16193.0 0.35249 0.518167

1017 230003_at Transcribed sequences AW779917 Hs.88156.0 0.35197 0.095261 -41017 230003_at Transcribed sequences AW779917 Hs.88156.0 0.35197 0.095261 -4

1018 225996_at Hs.21380.0 0.3507 6.0234051018 225996_at Hs.21380.0 0.3507 6.023405

1019 204697 s at CHGA chromogranin A NM_001275 g10800418 NP_001266 Chr:14q32 0.34944 2.08989 (parathyroid secretory protein 1) /// chromogranin A (parathyroid secretory protein 1 )1019 204697 s at CHGA chromogranin A NM_001275 g10800418 NP_001266 Chr: 14q32 0.34944 2.08989 (parathyroid secretory protein 1) /// chromogranin A (parathyroid secretory protein 1)

1020 226992. _at NOSTRIN nitric oxide synthase AK002203 Hs.10260.0 NP 443178 Chr:2q31.1 0.34873 0.095261 trafficking1020 226992. _at NOSTRIN nitric oxide synthase AK002203 Hs.10260.0 NP 443178 Chr: 2q31.1 0.34873 0.095261 trafficking

1021 231308. _at ANKFY1 ankyrin repeat and BG055427 Hs.301721.0 NP_057460 Chr:17p13.3 0.34793 0.095261 FYVE domain containing 11021 231308 . _at ANKFY1 ankyrin repeat and BG055427 Hs.301721.0 NP_057460 Chr: 17p13.3 0.34793 0.095261 FYVE domain containing 1

1022 231925. _at CDNA: FLJ23006 fis, AK026659 Hs.79881.0 — 0.34529 9.266777 clone LNG004141022 231925. _at CDNA: FLJ23006 fis, AK026659 Hs.79881.0 - 0.34529 9.266777 clone LNG00414

1023 227088. _at CDNA FLJ42757 fis, BF221547 Hs.16578.0 — Chr:4q27 0.34501 0.095261 clone BRAWH30017121023 227088. _at CDNA FLJ42757 fis, BF221547 Hs.16578.0 - Chr: 4q27 0.34501 0.095261 clone BRAWH3001712

1024 220084. _at C14orf105 chromosome 14 open NM_018168 g8922570 NP_060638 Chr:14q22.3 0.34467 1.051298 reading frame 105 1024 220084. _at C14orf105 chromosomes 14 open NM_018168 g8922570 NP_060638 Chr: 14q22.3 0.34467 1.051298 reading frame 105

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

1025 226974 at Transcribed sequence AL536553 Hs.3731.0 0.34446 0.095261 with weak similarity to protein sp:P391931025 226974 at Transcribed sequence AL536553 Hs.3731.0 0.34446 0.095261 with weak similarity to protein sp: P39193

(H. sapiens)(H. sapiens)

ALU6_HUMAN AIu subfamily SP sequence contamination warning entryALU6_HUMAN AI subfamily SP sequence contamination warning entry

1026 235183_at Clone A1479277 Hs.151275.0 0.34115 1.702877 IMAGE:5312689, mRNA1026 235183_at Clone A1479277 Hs.151275.0 0.34115 1.702877 IMAGE: 5312689, mRNA

1027 221874_at KIAA1324 mabal AB037745 Hs.104696.0 NP. _065826 Chr:1p13.3- 0.34005 0.095261 p13.21027 221874_at KIAA1324 mabal AB037745 Hs.104696.0 NP . _065826 Chr: 1p13.3- 0.34005 0.095261 p13.2

1028 242496_at — CDNA FLJ23728 fis, T57776 Hs.191094.0 — — 0.34001 0.779487 clone HEP142431028 242496_at - CDNA FLJ23728 fis, T57776 Hs.191094.0 - - 0.34001 0.779487 clone HEP14243

1029 231015 at KLF15 Kruppel-like factor 15 AW014734 Hs.157969.0 NP 054798 Chr:3q13-q21 0.33982 0.3536691029 231015 at KLF15 Kruppel-like factor 15 AW014734 Hs.157969.0 NP 054798 B: 3q13-q21 0.33982 0.353669

1030 232277_at SLC28A3 solute carrier family 28 AA643687 Hs.149425.0 NP. .071410 Chr:9q22.2 0.33924 1.901012 (sodium-coupled -4 nucleoside transporter), member 31030 232277_at SLC28A3 solute carrier family 28 AA643687 Hs.149425.0 NP. .071410 Chr: 9q22.2 0.33924 1.901012 (sodium-coupled -4 nucleoside transporter), member 3

1031 229222 at FLJ21963 FLJ21963 protein AI123815 Hs.16602.0 NP 078836 Chr:12q21.31 0.33908 0.1499111031 229222 at FLJ21963 FLJ21963 protein AI123815 Hs.16602.0 NP 078836 Chr: 12q21.31 0.33908 0.149911

1032 211682 x at UGT2B28 UDP AF177272 gl 3603475 NP_444267 Chr:4q13.3 0.33886 6.991358 glycosyltransferase 2 family, Polypeptide B28 /// UDP glycosyltransferase 2 family, Polypeptide B281032 211682 x at UGT2B28 UDP AF177272 gl 3603475 NP_444267 Chr: 4q13.3 0.33886 6.991358 glycosyltransferase 2 family, polypeptides B28 /// UDP glycosyltransferase 2 family, polypeptides B28

1033 230130_at SLIT2 slit homolog 2 AI692523 Hs.110373.1 NP. _004778 Chr:4p15.2 0.33729 0.625663 (Drosophila)1033 230130_at SLIT2 slit homolog 2 AI692523 Hs.110373.1 NP . _004778 Chr: 4p15.2 0.33729 0.625663 (Drosophila)

1034 232136_s_at CORTBP2 cortactin binding AB051545 Hs.293539.0 NP. ,219499 Chr:7q31 0.33673 1.051298 protein 21034 232136_s_at CORTBP2 cortactin binding AB051545 Hs.293539.0 NP . , 219499 Chr: 7q31 0.33673 1.051298 protein 2

1035 221172_at FLJ21075 hypothetica! protein NM_025031 gl 3376555 NP. _079307 Chr:7p12.3 0.3367 0.095261 FLJ210751035 221172_at FLJ21075 hypothetica! protein NM_025031 gl 3376555 NP. _079307 Chr: 7p12.3 0.3367 0.095261 FLJ21075

1036 206070 s at EPHA3 EphA3 /// EphA3 AF213459 gl 2003434 NP 005224 Chr:3p11.2 0.33646 0.3536691036 206070 s at EPHA3 EphA3 /// EphA3 AF213459 gl 2003434 NP 005224 Chr: 3p11.2 0.33646 0.353669

1037 242346_x_at — — BF222929 Hs.290585.0 — — 0.33563 0.201325 1037 242346_x_at - - BF222929 Hs.290585.0 - - 0.33563 0.201325

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-vaiue Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-vaiue Sample Number ID Protein ID Localization Change (%)

1038 209291_at ID4 inhibitor of DNA binding AW157094 Hs.34853.0 NPJ301537 Chr:6p22-p21 0.33561 0.095261 4, dominant negative helix-ioop-helix protein1038 209291_at ID4 inhibitor of DNA binding AW157094 Hs.34853.0 NPJ301537 Chr: 6p22-p21 0.33561 0.095261 4, dominant negative helix-ioop-helix protein

1039 209960_at Hs.809.0 0.33552 0.0952611039 209960_at Hs.809.0 0.33552 0.095261

1040 231031 at Similar to keratinocyte AI761573 Hs.164568.1 — Chr:9q21.11 0.33452 1.223189 growth factor-like protein, group IiI - human (LOC401517), mRNAAI761573 Hs.164568.1 - Chr: 9q21.11 0.33452 1.223189 growth factor-like protein, group IiI-human (LOC401517), mRNA

1041 220334_at RGS17 regulator of G-protein NM_012419 g8394185 NP_036551 Chr:6q25-q26 0.3318 10.20301 signalling 17 /// regulator of G-protein signailing 171041 220334_at RGS17 regulator of G-protein NM_012419 g8394185 NP_036551 Chr: 6q25-q26 0.3318 10.20301 signaling 17 /// regulatory of G-protein signaling 17

1042 201739_at SGK serum/glucocorticoid NM_005627 g5032090 NP_005618 Chr:6q23 0.3307 0.095261 regulated kinase /// serum/glucocorticoid regulated kinase1042 201739_at SGK serum / glucocorticoid NM_005627 g5032090 NP_005618 Chr: 6q23 0.3307 0.095261 regulated kinase /// serum / glucocorticoid regulated kinase

1043 207820 at ADH1A alcohol dehydrogenase NM_000667 gl 1496886 NPJ000658 Chr:4q21-q23 0.33061 0.149911 1A (class I), alpha Polypeptide /// alcohol § dehydrogenase 1A (class I), alpha Polypeptide1043 207820 at ADH1A alcohol dehydrogenase NM_000667 gl 1496886 NPJ000658 Chr: 4q21-q23 0.33061 0.149911 1A (class I), alpha polypeptides /// alcohol § dehydrogenase 1A (class I), alpha polypeptides

1044 238750 at Transcribed sequence AW083576 Hs.218707.0 0.33046 0.353669 with weak similarity to protein sp:P39190 (H.sapiens) ALU3_HUMAN AIu subfamily SB11044 238750 at Transcribed sequence AW083576 Hs.218707.0 0.33046 0.353669 with weak similarity to protein sp: P39190 (H.sapiens) ALU3_HUMAN AI subfamily SB1

Figure imgf000081_0001
Figure imgf000081_0001

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

1045 207048 at SLC6A11 solute carrier family 6 NM_014229 g7657586 NP_055044 Chr:3p25.3 0.32955 0.625663 (neurotransmitter transporter, GABA), member 11 /// solute carrier family 6 (neurotransmitter transporter, GABA), member 111045 207048 at SLC6A11 solute carrier family 6 NM_014229 g7657586 NP_055044 Chr: 3p25.3 0.32955 0.625663 (neurotransmitter transporter, GABA), member 11 /// solute carrier family 6 (neurotransmitter transporter, GABA), member 11

1046 227498_at — CDNA FLJ11723 fis, AI480314 Hs.196008.0 — — 0.32903 0.095261 clone HEMBA10053141046 227498_at - CDNA FLJ11723 fis, AI480314 Hs.196008.0 - - 0.32903 0.095261 clone HEMBA1005314

1047 229170_s_at TTC18 tetratricopeptide repeat AW024437 Hs.110667.0 NP_660153 Chr:10q22.3 0.3289 0.353669 domain 181047 229170_s_at TTC18 tetratricopeptide repeat AW024437 Hs.110667.0 NP_660153 Chr: 10q22.3 0.3289 0.353669 domain 18

1048 234834_at — — AF233648 Hs.272403.0 — — 0.32861 0.0952611048 234834_at - - AF233648 Hs.272403.0 - - 0.32861 0.095261

1049 233446_at — CDNA FLJ11655 fis, AU 145336 Hs.287436.0 — — 0.32841 0.149911 clone HEMBAI 0045541049 233446_at - CDNA FLJ11655 fis, AU 145336 Hs.287436.0 - - 0.32841 0.149911 clone HEMBAI 004554

1050 213933 at Hs.27860.0 0.32796 2.4891171050 213933 at Hs.27860.0 0.32796 2.489117

1051 232235 at NCAG1 NCAG1 AK021539 Hs.124673.1 NP 115536 Chr:18q22.1 0.32741 13.52851051 232235 at NCAG1 NCAG1 AK021539 Hs.124673.1 NP 115536 Chr: 18q22.1 0.32741 13.5285

1052 212840_at KIAA0794 KIAA0794 protein BG339560 Hs.127287.0 — 001052 212840_at KIAA0794 KIAA0794 protein BG339560 Hs.127287.0 - 00

Chr:3q29 0.32739 0.149911Chr: 3q29 0.32739 0.149911

1053 237263_at — Füll length insert cDNA BF590253 Hs.259664.0 — 0.32605 0.095261 clone YI54D041053 237263_at - Fill length insert cDNA BF590253 Hs.259664.0 - 0.32605 0.095261 clone YI54D04

1054 208269_s_at ADAM28 a disintegrin and NM_021777 gl 1496993 NP_055080 Chr:8p21.2 0.32532 0.095261 metalloproteinase domain 281054 208269_s_at ADAM28 a disintegrin and NM_021777 gl 1496993 NP_055080 Chr: 8p21.2 0.32532 0.095261 metalloproteinase domain 28

1055 234192_s_at GKAP1 G kinase anchoring AK026487 Hs.306855.0 NP_079487 Chr:9q22.1 0.3252 11.55252 protein 11055 234192_s_at GKAP1 G kinase anchoring AK026487 Hs.306855.0 NP_079487 Chr: 9q22.1 0.3252 11.55252 protein 1

1056 207919_at ART1 ADP-ribosyltransferase NM_004314 g4757783 NP_004305 Chr.11p15 0.32406 0.095261 1 /// ADP- ribosyltransferase 11056 207919_at ART1 ADP-ribosyltransferase NM_004314 g4757783 NP_004305 Chr.11p15 0.32406 0.095261 1 /// ADP-ribosyltransferase 1

1057 232570_s_at ADAM33 a disintegrin and AL356755 Hs.173716.0 NPJD79496 Chr:20p13 0.32335 2.08989 metalloproteinase domain 331057 232570_s_at ADAM33 a disintegrin and AL356755 Hs.173716.0 NPJD79496 Chr: 20p13 0.32335 2.08989 metalloproteinase domain 33

1058 228885_at MAMDC2 MAM domain AI862120 Hs.131987.0 NP_694999 Chr.9q21.13- 0.32232 1.380569 containing 2 q21.21058 228885_at MAMDC2 MAM domain AI862120 Hs.131987.0 NP_694999 Chr.9q21.13- 0.32232 1.380569 containing 2 q21.2

1059 205433_at BCHE butyrylcholinesterase /// NM_000055 g4557350 NP_000046 Chr:3q26.1- 0.32111 0.201325 butyrylcholinesterase q26.21059 205433_at BCHE butyrylcholinesterase /// NM_000055 g4557350 NP_000046 Chr: 3q26.1- 0.32111 0.201325 butyrylcholinesterase q26.2

1060 236141 at — Transcribed sequences AA156933 Hs.177532.0 — — 0.32079 0.779487 1060 236141 at - Transcribed sequences AA156933 Hs.177532.0 - - 0.32079 0.779487

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1061 234062_at — CDNA FLJ12400 fis, AK022462 Hs.296721.0 — — 0.31743 0.779487 clone MAMMAI 0027821061 234062_at - CDNA FLJ12400 fis, AK022462 Hs.296721.0 - - 0.31743 0.779487 clone MAMMAI 002782

1062 219093_at FLJ20701 hypothetical protein NM_017933 g8923631 NP_060403 Chr:2q37.1 0.316 0.095261 FLJ207011062 219093_at FLJ20701 hypothetical protein NM_017933 g8923631 NP_060403 Chr: 2q37.1 0.316 0.095261 FLJ20701

1063 228507_at PDE3A Phosphodiesterase 3A, AI742043 Hs.24305.0 NP_000912 Chr:12p12 0.31515 6.023405 cGMP-inhibited1063 228507_at PDE3A Phosphodiesterase 3A, AI742043 Hs.24305.0 NP_000912 Chr: 12p12 0.31515 6.023405 cGMP-inhibited

1064 208335_s_at FY Duffy blood group /// NM_002036 g4503818 NP_002027 Chr:1q21-q22 0.3139 1.380569 Duffy blood group1064 208335_s_at FY Duffy blood group /// NM_002036 g4503818 NP_002027 Chr: 1q21-q22 0.3139 1.380569 Duffy blood group

1065 212865_s_at COL14A1 Collagen, type XIV, BF449063 Hs.36131.0 — Chr:8q23 0.31389 1.380569 alpha 1 (undulin)1065 212865_s_at COL14A1 Collagen, type XIV, BF449063 Hs.36131.0 - Chr: 8q23 0.31389 1.380569 alpha 1 (undulin)

1066 235666 at Transcribed sequence AA903473 Hs.153717.0 0.31252 0.149911 with moderate similarity to protein sp:P391941066 235666 at Transcribed sequence AA903473 Hs.153717.0 0.31252 0.149911 with moderate similarity to protein sp: P39194

(H.sapiens)(H.sapiens)

ALU7_HUMAN AIu subfamily SQ sequence 00 contamination warning entryALU7_HUMAN AIu subfamily SQ sequence 00 contamination warning entry

1067 222102 at GSTA3 glutathione S- NM_000847 Hs.102484.0 NP_000838 Chr:6p12.1 0.31072 0.095261 transferase A3 /// glutathione S- transferase A31067 222102 at GSTA3 glutathione S- NM_000847 Hs.102484.0 NP_000838 Chr: 6p12.1 0.31072 0.095261 transferase A3 /// glutathione S-transferase A3

1068 202768 at FOSB FBJ murine NM_006732 g5803016 NP_006723 Chr:19q13.32 0.31054 2.814919 osteosarcoma viral oncogene homolog B /// FBJ murine osteosarcoma viral oncogene homolog B1068 202768 at FOSB FBJ murine NM_006732 g5803016 NP_006723 Chr: 19q13.32 0.31054 2.814919 osteosarcoma viral oncogene homolog B /// FBJ murine osteosarcoma viral oncogene homolog B

1069 225516_ at Hs.93961.0 0.30931 1.7028771069 225516_ at Hs.93961.0 0.30931 1.702877

1070 228739. _at Cilia-associated protein AH 39413 Hs.27092.0 0.30902 11.55252 (CYSI) mRNA, complete cds1070 228739. _at Cilia-associated protein AH 39413 Hs.27092.0 0.30902 11.55252 (CYSI) mRNA, complete cds

1071 229160. _at FLJ33516 hypothetical protein AI967987 Hs.10653.0 NP_689636 Chr:xq22.3 0.30874 0.095261 FLJ335161071 229160 . _at FLJ33516 hypothetical protein AI967987 Hs.10653.0 NP_689636 Chr: xq22.3 0.30874 0.095261 FLJ33516

1072 239829. _at — — BE221351 Hs.49727.0 — — 0.30863 0.095261 1072 239829. _at - - BE221351 Hs.49727.0 - - 0.30863 0.095261

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1073 244402_at KIAA0436 putative L-type neutral AW605424 Hs.130056.0 — Chr:2p22.1 0.30562 4.75532 amino acid transporter1073 244402_at KIAA0436 putative L-type neutral AW605424 Hs.130056.0 - Chr: 2p22.1 0.30562 4.75532 amino acid transporter

1074 237151_s_at — CDNA FLJ44327 fis, BF433885 Hs.128245.0 — Chr:2q14.2 0.30488 0.095261 clone TRACH30028661074 237151_s_at - CDNA FLJ44327 fis, BF433885 Hs.128245.0 - Chr: 2q14.2 0.30488 0.095261 clone TRACH3002866

1075 229580_at — Transcribed sequences R71596 Hs.7413.0 — — 0.30208 4.1741891075 229580_at - Transcribed sequences R71596 Hs.7413.0 - - 0.30208 4.174189

1076 219937 at TRHDE thyrotropin-releasing NM_013381 g7019560 NP_037513 Chr:12q15-q21 0.30148 12.53374 hormone degrading ectoenzyme /// thyrotropin-releasing hormone degrading ectoenzyme1076 219937 at TRHDE thyrotropin-releasing NM_013381 g7019560 NP_037513 Chr: 12q15-q21 0.30148 12.53374 hormone-degrading ectoenzymes /// thyrotropin-releasing hormone-degrading ectoenzymes

1077 227559 at Transcribed sequence AL521129 Hs.5232.1 0.30137 0.095261 with strong similarity to protein ref:NP_054884.11077 227559 at Transcribed sequence AL521129 Hs.5232.1 0.30137 0.095261 with strong similarity to protein ref: NP_054884.1

(H.sapiens) HSPC125 protein [Homo sapiens] 90(H.sapiens) HSPC125 protein [Homo sapiens] 90

1078 231038 s at Transcribed sequence AI309438 Hs.108689.1 0.3009 0.353669 with strong similarity to protein ref:NP_078795.1 (H.sapiens) hypothetical protein FLJ 137251078 231038 s at Transcribed sequence AI309438 Hs.108689.1 0.3009 0.353669 with similar similarity to protein ref: NP_078795.1 (H.sapiens) hypothetical protein FLJ 13725

1079 222112. _at EPS15L1 epidermal growth factor AV710549 Hs.147176.1 NP_067058 Chr:19p13.12 0.30078 0.095261 receptor pathway Substrate 15-like 11079 222112. _at EPS15L1 epidermal growth factor AV710549 Hs.147176.1 NP_067058 Chr: 19p13.12 0.30078 0.095261 receptor pathway Substrate 15-like 1

1080 204689_ _at HHEX hematopoietically NM_001529 gl 0835016 NP_001520 Chr:10q23.33 0.30061 0.095261 expressed homeobox /// hematopoietically expressed homeobox1080 204689_ _at HHEX hematopoietically NM_001529 gl 0835016 NP_001520 Chr: 10q23.33 0.30061 0.095261 expressed homeobox /// hematopoietically expressed homeobox

1081 236038. _at — — N50714 Hs.42746.0 — — 0.30045 1.901012 1081 236038. _at - - N50714 Hs.42746.0 - - 0.30045 1.901012

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

1082 229641 at Transcribed sequence BG252802 Hs.49943.0 0.30036 2.08989 with moderate similarity to protein sp:P39192 (H.sapiens) ALU5_HUMAN AIu subfamily SC sequence contamination warning entry1082 229641 at Transcribed sequence BG252802 Hs.49943.0 0.30036 2.08989 with moderate similarity to protein sp: P39192 (H.sapiens) ALU5_HUMAN AI subfamily SC sequence contamination warning entry

1083 232878_ _at — CDNA FLJ46792 fis, AU 143891 Hs.274227.0 — Chr:15q26.2 0.29998 0.095261 clone TRACH30295201083 232878_ _at - CDNA FLJ46792 fis, AU 143891 Hs.274227.0 - Chr: 15q26.2 0.29998 0.095261 clone TRACH3029520

1084 228728_ _at FLJ21986 hypothetical protein BF724137 Hs.24144.0 NP. .079189 Chr:7q31.32 0.29931 0.095261 FLJ219861084 228728_ _at FLJ21986 hypothetical protein BF724137 Hs.24144.0 NP. .079189 Chr: 7q31.32 0.29931 0.095261 FLJ21986

1085 223784, _at NX17 kidney-specific AF229179 g9957753 NP. _065716 Chr:xp22 0.29744 0.353669 membrane protein1085 223784 , _at NX17 kidney-specific AF229179 g9957753 NP. _065716 Chr: xp22 0.29744 0.353669 membrane protein

1086 228293_ at LOC91614 novel 58.3 KDA protein AJ245600 Hs.180545.0 NP 631899 Chr:11p13 0.29507 0.2013251086 228293_ at LOC91614 novel 58.3 KDA protein AJ245600 Hs.180545.0 NP 631899 Chr: 11p13 0.29507 0.201325

1087 223646_ _s_at CYorf15B chromosome Y open AF332225 gl 3161083 NP. .115965 Chryqi 1.222 0.29462 0.095261 reading frame 15B1087 223646_ _s_at CYorf15B chromosomes Y open AF332225 gl 3161083 NP. .115965 Chryqi 1.222 0.29462 0.095261 reading frame 15B

1088 210197. ≠ ITPK1 inositol 1 ,3,4- BC003622 gl 3177675 NP. _055031 Chr:14q31 0.29259 0.095261 triphosphate 5/6 kinase1088 210197 . ≠ ITPK1 inositol 1, 3,4- BC003622 gl 3177675 NP. _055031 Chr: 14q31 0.29259 0.095261 triphosphate 5/6 kinase

1089 239539_ _at AI291210 Hs.149795.0 0.29236 0.2013251089 239539_ _at AI291210 Hs.149795.0 0.29236 0.201325

1090 225491. _at Hs.4774.0 0.29187 0.5181671090 225491. _at Hs.4774.0 0.29187 0.518167

1091 205380. .at PDZK1 PDZ domain containing NM_002614 g4505702 NP. _002605 Chr:1q21 0.29103 3.612633 1 /// PDZ domain 1 1 11091 205380 . PDZK1 PDZ domain containing NM_002614 g4505702 NP . _002605 Chr: 1q21 0.29103 3.612633 1 /// PDZ domain 1 1 1

Figure imgf000085_0001
Figure imgf000085_0001

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1095 206204_at GRB14 growth factor receptor- NM_004490 g4758477 NP_004481 Chr:2q22-q24 0.2871 4.436744 bound protein 14 /// growth factor receptor- bound protein 141095 206204_at GRB14 growth factor receptor NM_004490 g4758477 NP_004481 Chr: 2q22-q24 0.2871 4.436744 bound protein 14 /// growth factor receptor-bound protein 14

1096 236952_at — Transcribed sequences AI309861 Hs.150896.0 — — 0.28686 9.2667771096 236952_at - Transcribed sequences AI309861 Hs.150896.0 - - 0.28686 9.266777

1097 204931_at TCF21 transcription factor 21 NM_003206 g4507394 NPJ303197 Chr:6pter-qter 0.28566 0.095261 /// transcription factor 211097 204931_at TCF21 transcription factor 21 NM_003206 g4507394 NPJ303197 Chr: 6pter-qter 0.28566 0.095261 /// transcription factor 21

1098 208096_s_at COL21A1 collagen, type XXI, NM_030820 gl 3540617 NPJ 10447 Chr:6p12.3- 0.28495 0.353669 alpha 1 /// collagen, p11.2 type XXI, alpha 11098 208096_s_at COL21A1 collagen, type XXI, NM_030820 gl 3540617 NPJ 10447 Chr: 6p12.3- 0.28495 0.353669 alpha 1 /// collagen, p11.2 type XXI, alpha 1

1099 238658_at — CDNA FLJ34787 fis, AA832474 Hs.25851.0 — — 0.2839 0.095261 clone NT2NE20047401099 238658_at - CDNA FLJ34787 fis, AA832474 Hs.25851.0 - - 0.2839 0.095261 clone NT2NE2004740

1100 205103_at CROC4 transcriptional activator NM_006365 g5453624 NP_006356 Chr:1q23.1 0.28165 0.095261 of the c-fos promoter /// transcriptional activator of the c-fos promoter 001100 205103_at CROC4 transcriptional activator NM_006365 g5453624 NP_006356 Chr: 1q23.1 0.28165 0.095261 of the c-fos promoter /// transcriptional activator of the c-fos promoter 00

1101 209469_at GPM6A glycoprotein M6A BF939489 Hs.75819.0 NP_005268 Chr:4q34 0.28104 0.149911 Ul1101 209469_at GPM6A glycoprotein M6A BF939489 Hs.75819.0 NP_005268 Chr: 4q34 0.28104 0.149911 Ul

1102 209074 s at g4886486 0.27968 0.7794871102 209074 s at g4886486 0.27968 0.779487

1103 222353_at LIMD1 LIM domains AV720842 Hs.282435.1 NP_055055 Chr:3p21.3 0.27777 0.149911 containing 11103 222353_at LIMD1 LIM domains AV720842 Hs.282435.1 NP_055055 Chr: 3p21.3 0.27777 0.149911 containing 1

1104 207016 s at ALDH 1A2 aldehyde AB015228 g3970845 NP_003879 Chr:15q21.2 0.27736 8.153992 dehydrogenase 1 family, member A2 /// aldehyde dehydrogenase 1 family, member A21104 207016 s at ALDH 1A2 aldehydes AB015228 g3970845 NP_003879 Chr: 15q21.2 0.27736 8.153992 dehydrogenase 1 family, member A2 /// aldehyde dehydrogenase 1 family, member A2

1105 220829 s at B3GALT1 UDP-Gal:betaGlcNAc NM_020981 gl 0304986 NP_066191 Chr:2q31.1 0.27697 0.095261 beta 1,3- galactosyltransferase, Polypeptide 1 /// UDP- Gal:betaGlcNAc beta 1 ,3- galactosyltransferase, Polypeptide 1 1105 220829 s at B3GALT1 UDP-Gal: betaGlcNAc NM_020981 gl 0304986 NP_066191 Chr: 2q31.1 0.27697 0.095261 beta 1,3-galactosyltransferase, polypeptides 1 /// UDP-Gal: betaGlcNAc beta 1, 3-galactosyltransferase, polypeptides 1

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1106 223467_at RASD1 RAS, dexamethasone- AF069506 g4959037 NP_057168 Chr:17p11.2 0.27636 0.095261 induced 11106 223467_at RASD1 RAS, dexamethasone AF069506 g4959037 NP_057168 Chr: 17p11.2 0.27636 0.095261 induced 1

1107 226388_at TCEA3 transcription elongation AI675780 Hs.94952.0 NP_003187 Chr:1p36.11 0.27633 6.023405 factor A (SII), 31107 226388_at TCEA3 transcription elongation AI675780 Hs.94952.0 NP_003187 Chr: 1p36.11 0.27633 6.023405 factor A (SII), 3

1108 221584 s at KCNMA1 potassium large U11058 g7914977 NP_002238 Chr:10q22-q23 0.27586 0.353669 conductance calcium- activated Channel, subfamily M, alpha member 1 /// potassium large conductance calcium-activated Channel, subfamily M, alpha member 11108 221584 s at KCNMA1 potassium large U11058 g7914977 NP_002238 Chr: 10q22-q23 0.27586 0.353669 conductance calcium-activated channel, subfamily M, alpha member 1 /// potassium large conductance calcium-activated channel, subfamily M, alpha member 1

1109 236261 _at OSBPL6 oxysterol binding AI949389 Hs.18067.0 NP_115912 Chr:2q31-q32.1 0.27579 0.149911 protein-like 61109 236261 _at OSBPL6 oxysterol binding AI949389 Hs.18067.0 NP_115912 Chr: 2q31-q32.1 0.27579 0.149911 protein-like 6

1110 222764 at ASRGL1 asparaginase like 1 AI928342 Hs.7331.0 NP 079356 Chr:11q12.3 0.27434 6.0234051110 222764 at ASRGL1 asparaginase like 1 AI928342 Hs.7331.0 NP 079356 Chr: 11q12.3 0.27434 6.023405

1111 227202 at AW072790 Hs.143434.2 0.27402 0.7794871111 227202 at AW072790 Hs.143434.2 0.27402 0.779487

1112 226591 _at Hs.22543.0 0.27396 9.266777 c

Figure imgf000087_0001
\1112 226591 _at Hs.22543.0 0.27396 9.266777 c
Figure imgf000087_0001
\

1113 205922_at VNN2 vanin 2 /// vanin 2 NM_004665 g4759313 NP 004656 Chr:6q23-q24 0.27362 0.5181671113 205922_at VNN2 vanin 2 /// vanin 2 NM_004665 g4759313 NP 004656 Chr: 6q23-q24 0.27362 0.518167

1114 204754 at HLF hepatic leukemia factor W60800 Hs.250692.0 NP 002117 Chr:17q22 0.27304 0.0952611114 204754 at HLF hepatic leukemia factor W60800 Hs.250692.0 NP 002117 Chr: 17q22 0.27304 0.095261

1115 206932_at CH25H cholesterol 25- NM_003956 g4502498 NPJD03947 Chr:10q23 0.27303 0.095261 hydroxylase /// cholesterol 25- hydroxylase1115 206932_at CH25H cholesterol 25-NM_003956 g4502498 NPJD03947 Chr: 10q23 0.27303 0.095261 hydroxylase /// cholesterol 25-hydroxylase

1116 241694_at PKHD1 polycystic kidney and AI770005 Hs.280977.0 NP_619639 Chr:6p12.2 0.27242 0.095261 hepatic disease 1 (autosomal recessive)1116 241694_at PKHD1 polycystic kidney and AI770005 Hs.280977.0 NP_619639 Chr: 6p12.2 0.27242 0.095261 hepatic disease 1 (autosomal recessive)

1117 228504_at SCN7A sodium Channel, AI828648 Hs.23804.0 NP_002967 Chr:2q21-q23 0.27232 0.095261 voltage-gated, type VII, alpha1117 228504_at SCN7A sodium Channel, AI828648 Hs.23804.0 NP_002967 Chr: 2q21-q23 0.27232 0.095261 voltage-gated, type VII, alpha

1118 219949_at LRRC2 leucine rieh repeat NM_024512 gl 3378142 NP_078788 Chr:3p21.31 0.27211 6.991358 containing 2 /// leucine rieh repeat containing 21118 219949_at LRRC2 leucine repeat repeat NM_024512 gl 3378142 NP_078788 Chr: 3p21.31 0.27211 6.991358 containing 2 /// leucine rieh repeat containing 2

1119 213436_at CNR1 cannabinoid reeeptor 1 U73304 Hs.75110.1 NP_001831 Chr:6q14-q15 0.26934 3.612633 (brain) 1119 213436_at CNR1 cannabinoid reeeptor 1 U73304 Hs.75110.1 NP_001831 Chr: 6q14-q15 0.26934 3.612633 (brain)

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1120 243951_at ABCB1 ATP-binding cassette, AA887211 Hs.129467.0 NP_000918 Chr:7q21.1 0.26915 9.266777 sub-family B (MDR/TAP), member 11120 243951_at ABCB1 ATP-binding cassette, AA887211 Hs.129467.0 NP_000918 Chr: 7q21.1 0.26915 9.266777 sub-family B (MDR / TAP), member 1

1121 242239_at NOPD1 nucleolar protein AW970888 Hs.293147.0 NP_872349 Chr:10p13 0.26839 6.023405 (NOL1/NOP2/sun) and PUA domains 11121 242239_at NOPD1 nucleolar protein AW970888 Hs.293147.0 NP_872349 Chr: 10p13 0.26839 6.023405 (NOL1 / NOP2 / sun) and PUA domains 1

1122 210258_at RGS 13 regulator of G-protein AF030107 g2598184 NP_002918 Chr:1q31.1 0.26762 11.06357 signalling 131122 210258_at RGS 13 regulator of G-protein AF030107 g2598184 NP_002918 Chr: 1q31.1 0.26762 11.06357 signaling 13

1123 244740_at MGC9913 hypothetical protein BE855713 Hs.23133.1 — Chr:19q13.43 0.26725 6.023405 MGC99131123 244740_at MGC9913 hypothetical protein BE855713 Hs.23133.1 - Chr: 19q13.43 0.26725 6.023405 MGC9913

1124 204719 at ABCA8 ATP-binding cassette, NM_007168 g6005700 NPJD09099 Chr:17q24 0.26719 0.095261 sub-family A (ABCI), member 8 /// ATP- binding cassette, sub- family A (ABCI), member 81124 204719 at ABCA8 ATP-binding cassette, NM_007168 g6005700 NPJD09099 Chr: 17q24 0.26719 0.095261 sub-family A (ABCI), member 8 /// ATP-binding cassette, subfamily A (ABCI), member 8

1125 237659_at FLJ40288 hypothetical protein AW134826 Hs.227092.0 NP_775953 Chr:7q32.3 0.26701 0.095261 FLJ40288 90 -41125 237659_at FLJ40288 hypothetical protein AW134826 Hs.227092.0 NP_775953 Chr: 7q32.3 0.26701 0.095261 FLJ40288 90 -4

1126 222257_s_at ACE2 angiotensin I AK026461 Hs.178098.1 NP_068576 Chr:xp22 0.26694 0.625663 Converting enzyme (peptidyl-dipeptidase A) 21126 222257_s_at ACE2 angiotensin I AK026461 Hs.178098.1 NP_068576 Chr: xp22 0.26694 0.625663 Converting enzyme (peptidyl-dipeptidase A) 2

1127 211451_s„at KCNJ4 potassium inwardly- U24056 g775226 NPJ304972 Chr:22q13.1 0.26663 0.095261 rectifying Channel, subfamily J, member 41127 211451_s " at KCNJ4 potassium inwardly- U24056 g775226 NPJ304972 Chr: 22q13.1 0.26663 0.095261 rectifying Channel, subfamily J, member 4

1128 211748_x_at PTGDS Prostaglandin D2 BC005939 gl 3543567 NP_000945 Chr:9q34.2- 0.26591 0.625663 synthase 21 kDa (brain) q34.3 /// Prostaglandin D2 synthase 21 kDa (brain)1128 211748_x_at PTGDS prostaglandin D2 BC005939 gl 3543567 NP_000945 Chr: 9q34.2- 0.26591 0.625663 synthase 21 kDa (brain) q34.3 /// prostaglandin D2 synthase 21 kDa (brain)

1129 228750 at — Transcribed sequences AI693516 Hs.28625.0 — — 0.26364 1.0512981129 228750 at - Transcribed sequences AI693516 Hs.28625.0 - - 0.26364 1.051298

1130 207062_at IAPP islet amyloid NM_000415 g4557654 NP_000406 Chr:12p12.3- 0.26125 1.223189 Polypeptide /// islet p12.1 amyloid Polypeptide1130 207062_at IAPP islet amyloid NM_000415 g4557654 NP_000406 Chr: 12p12.3- 0.26125 1.223189 polypeptides /// islet p12.1 amyloid polypeptides

1131 229376_at — Transcribed sequences BF223556 Hs.107987.0 — — 0.2611 4.6400661131 229376_at - Transcribed sequences BF223556 Hs.107987.0 - - 0.2611 4.640066

1132 207802_at g5174674 0.26046 7.646445 1132 207802_at g5174674 0.26046 7.646445

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1133 208292_at BMP10 boπe morphogenetic NM_014482 g7656927 NP_055297 Chr:2p13.3 0.26033 0.095261 protein 10 /// bone morphogenetic protein 101133 208292_at BMP10 boπe morphogenetic NM_014482 g7656927 NP_055297 Chr: 2p13.3 0.26033 0.095261 protein 10 /// bone morphogenetic protein 10

1134 209763_at CHRDL1 chordin-like 1 AL049176 Hs.82223.0 NP 660277 Chr:xq23 0.25968 2.8149191134 209763_at CHRDL1 chordin-like 1 AL049176 Hs.82223.0 NP 660277 Chr: xq23 0.25968 2.814919

1135 241703_at RPIB9 Rap2-binding protein 9 AI928037 Hs.158832.0 NP 612147 Chr:7q21.13 0.25922 0.0952611135 241703_at RPIB9 Rap2-binding protein 9 AI928037 Hs.158832.0 NP 612147 Chr: 7q21.13 0.25922 0.095261

1136 232059_at DSCAML1 Down Syndrome cell AI433419 Hs.233023.0 NPJ365744 Chr:11q23 0.25909 6.023405 adhesion molecule like 11136 232059_at DSCAML1 Down Syndrome cell AI433419 Hs.233023.0 NPJ365744 Chr: 11q23 0.25909 6.023405 adhesion molecule like 1

1137 237328_at — Transcribed sequences AI927063 Hs.160711.0 — — 0.25678 11.552521137 237328_at - Transcribed sequences AI927063 Hs.160711.0 - - 0.25678 11.55252

1138 206254_at EGF epidermal growth factor NM_001963 g6031163 NP_001954 Chr:4q25 0.25591 0.353669 (beta-urogastrone) /// epidermal growth factor (beta-urogastrone)1138 206254_at EGF epidermal growth factor NM_001963 g6031163 NP_001954 Chr: 4q25 0.25591 0.353669 (beta-urogastrone) /// epidermal growth factor (beta-urogastrone)

1139 222162 s at ADAMTS1 a disintegrin-like and AK023795 Hs.8230.1 NP_008919 Chr:21q21.2 0.25526 0.095261 metalloprotease (reprolysin type) with1139 222162 s at ADAMTS1 a disintegrin-like and AK023795 Hs.8230.1 NP_008919 Chr: 21q21.2 0.25526 0.095261 metalloprotease (reprolysin type) with

00 thrombospondin type 1 90 motif, 1 /// a disintegrin- like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 100 thrombospondin type 1 90 motif, 1 /// a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1

1140 234307. _s_at KIF26A kinesin family member AK026406 Hs.134970.1 — Chr:14q32.33 0.25204 0.353669 26A1140 234307. _s_at KIF26A kinesin family member AK026406 Hs.134970.1 - Chr: 14q32.33 0.25204 0.353669 26A

1141 205620. „at F10 coagulation factor X /// NM_000504 g9961350 NPJD00495 Chr:13q34 0.25032 0.095261 coagulation factor X1141 205620. "at F10 coagulation factor X /// NM_000504 g9961350 NPJD00495 Chr: 13q34 0.25032 0.095261 coagulation factor X

1142 218949_ _s_at QRSL1 glutaminyl-tRNA NM_018292 g8922807 NP_060762 Chr:6q21 0.24966 7.646445 synthase (glutamine- hydrolyzing)-like 1 1142 218949_ _s_at QRSL1 glutaminyl-tRNA NM_018292 g8922807 NP_060762 Chr: 6q21 0.24966 7.646445 synthase (glutamine-hydrolyzing) -like 1

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

1143 240510 at Transcribed sequence H66741 Hs.38540.0 0.2485 0.095261 with weak similarlty to protein

Figure imgf000090_0001
(H. sapiens) neuronal thread protein [Homo sapiens]1143 240510 at Transcribed sequence H66741 Hs.38540.0 0.2485 0.095261 with weak similarity to protein
Figure imgf000090_0001
(H. sapiens) neuronal thread protein [Homo sapiens]

1144 232639 at Hs.225108.0 0.24803 0.0952611144 232639 at Hs.225108.0 0.24803 0.095261

1145 206030 at ASPA aspartoacylase NM_000049 g4557334 NP_000040 Chr:17pter-p13 0.24794 0.095261 (aminoacylase 2, Canavan disease) /// aspartoacylase (aminoacylase 2, Canavan disease)1145 206030 at ASPA aspartoacylase NM_000049 g4557334 NP_000040 Chr: 17pter-p13 0.24794 0.095261 (aminoacylase 2, Canavan disease) /// aspartoacylase (aminoacylase 2, Canavan disease)

1146 214876_s_at TUBGCP5 tubulin, gamma AI628925 Hs.31476.1 NP. _443135 Chr:15q11.2 0.24712 0.095261 complex associated protein 51146 214876_s_at TUBGCP5 tubulin, gamma AI628925 Hs.31476.1 NP . _443135 Chr: 15q11.2 0.24712 0.095261 complex associated protein 5

1147 207192_at DNASE1 L2 deoxyribonuclease I- NM_001374 g4503346 NP_ _001365 Chr:16p13.3 0.24621 0.095261

Figure imgf000090_0002
deoxyribonuclease I- iike 21147 207192_at DNASE1 L2 deoxyribonuclease I- NM_001374 g4503346 NP_ _001365 Chr: 16p13.3 0.24621 0.095261
Figure imgf000090_0002
deoxyribonuclease I-iike 2

1148 205624_at CPA3 carboxypeptidase A3 NM_001870 g4503000 NP_ .001861 Chr:3q21-q25 0.24512 10.20301 (mast cell) /// carboxypeptidase A3 (mast cell)1148 205624_at CPA3 carboxypeptidase A3 NM_001870 g4503000 NP_ .001861 Chr: 3q21-q25 0.24512 10.20301 (mast cell) /// carboxypeptidase A3 (mast cell)

1149 205083 at AOX1 aldehyde oxidase 1 NM 001159 g6598319 NP 001150 Chr:2q33 0.24494 0.0952611149 205083 at AOX1 aldehyde oxidase 1 NM 001159 g6598319 NP 001150 Chr: 2q33 0.24494 0.095261

1150 242164_s_at LRIG2 leucine-rich repeats BF433654 Hs.309305.0 Chr:1p13.1 0.24492 0.095261 and immunoglobulin- like domains 21150 242164_s_at LRIG2 leucine-rich repeats BF433654 Hs.309305.0 Chr: 1p13.1 0.24492 0.095261 and immunoglobulin- like domains 2

1151 206350_at APCS amyloid P component, NM_001639 g4502132 NP. _001630 Chr.1q21-q23 0.24474 0.353669 serum /// amyloid P component, serum1151 206350_at APCS amyloid P component, NM_001639 g4502132 NP. _001630 Chr.1q21-q23 0.24474 0.353669 serum /// amyloid P component, serum

1152 237364_at Clone BF432855 Hs.189885.0 0.24442 0.201325 IMAGE:5738452, mRNA 1152 237364_at Clone BF432855 Hs.189885.0 0.24442 0.201325 IMAGE: 5738452, mRNA

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

1153 204965 at GC group-specific NM_000583 g9845254 NP_000574 Chr:4q12-q13 0.24091 5.685182 component (vitamin D binding protein) /// group-specific component (vitamin D binding protein)1153 204965 at GC group-specific NM_000583 g9845254 NP_000574 Chr: 4q12-q13 0.24091 5.685182 component (vitamin D binding protein) /// group-specific component (vitamin D binding protein)

1154 205305 at FGL1 fibrinogen-like 1 NM 004467 g4758371 NP 004458 Chr:8p22-p21.3 0.24081 2.4891171154 205305 at FGL1 fibrinogen-like 1 NM 004467 g4758371 NP 004458 Chr: 8p22-p21.3 0.24081 2.489117

1155 231785_ _at NTF5 neurotrophin 5 NM_006179 Hs.266902.0 NP_ _006170 Chr:19q13.3 0.23989 0.149911 (neurotrophin 4/5)1155 231785_at NTF5 neurotrophin 5 NM_006179 Hs.266902.0 NP_ _006170 Chr: 19q13.3 0.23989 0.149911 (neurotrophin 4/5)

1156 202994 s at FBLN1 fibulin 1 Z95331 Hs.79732.0 NP 001987 Chr:22q13.31 0.23667 0.1499111156 202994 s at FBLN1 fibulin 1 Z95331 Hs.79732.0 NP 001987 Chr: 22q13.31 0.23667 0.149911

1157 205363 at BBOX1 butyrobetaine NM_003986 g4502368 NP_003977 Chr:11p14.2 0.23298 3.612633 (gamma), 2- oxoglutarate dioxygenase (gamma- butyrobetaine hydroxylase) 1 /// butyrobetaine (gamma), 2- O oxogl utarate dioxygenase (gamma- butyrobetaine hydroxylase) 11157 205363 at BBOX1 butyrobetaine NM_003986 g4502368 NP_003977 Chr: 11p14.2 0.23298 3.612633 (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 /// butyrobetaine (gamma), 2-oxoglate dioxygenase (gamma-butyrobetaine hydroxylase) 1

1158 211413 s at PADI4 peptidyl arginine AF229067 g8980666 NP_036519 Chr:1 p36.13 0.23278 0.095261 deiminase, type IV1158 211413 s at PADI4 peptidyl arginine AF229067 g8980666 NP_036519 Chr: 1 p36.13 0.23278 0.095261 deiminase, type IV

1159 206610 s at F11 coagulation factor Xl NM_000128 g9961351 NP_000119 Chr:4q35 0.23267 3.612633 (plasma thromboplastin antecedent) /// coagulation factor Xl (plasma thromboplastin antecedent)1159 206610 s at F11 coagulation factor Xl NM_000128 g9961351 NP_000119 Chr: 4q35 0.23267 3.612633 (plasma thromboplastin antecedent) /// coagulation factor Xl (plasma thromboplastin antecedent)

1160 227827_ _at Clone AW138143 Hs.71721.0 0.23239 1.702877 IMAGE:4791597, mRNA1160 227827_ _at Clone AW138143 Hs.71721.0 0.23239 1.702877 IMAGE: 4791597, mRNA

1161 209869_ _at ADRA2A adrenergic, alpha-2A-, AF284095 gl 3447750 NP_000672 Chr:10q24-q26 0.2306 7.646445 receptor /// adrenergic, alpha-2A-, receptor 1161 209869_at ADRA2A adrenergic, alpha-2A-, AF284095 gl 3447750 NP_000672 Chr: 10q24-q26 0.2306 7.646445 receptor /// adrenergic, alpha-2A, receptor

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change ""-Sample Number ID Protein ID Localization Change "" -

1162 235649 at ADAMTS8 a disintegrin-like and AW207389 Hs.126758.0 NP_008968 Chr:11q25 0.23039 6.023405 metalloprotease (reprolysin type) with thrombospondin type 1 motif, 81162 235649 at ADAMTS8 a disintegrin-like and AW207389 Hs.126758.0 NP_008968 Chr: 11q25 0.23039 6.023405 metalloprotease (reprolysin type) with thrombospondin type 1 motif, 8

1163 243171 at LOC389556 AA570178 Hs.105944.0 Chr:7q32.3 0.22954 1.051298 (LOG389556), mRNA1163 243171 at LOC389556 AA570178 Hs.105944.0 Chr: 7q32.3 0.22954 1.051298 (LOG389556), mRNA

1164 205886 at REG1B regenerating islet- NM_006507 gl 0835247 NP_006498 Chr:2p12 0.22862 3.62324 derived 1 beta (pancreatic stone protein, pancreatic thread protein) /// regenerating islet- derived 1 beta (pancreatic stone protein, pancreatic thread protein)1164 205886 at REG1B regenerating islet NM_006507 gl 0835247 NP_006498 Chr: 2p12 0.22862 3.62324 derived 1 beta (pancreatic stone protein, pancreatic thread protein) /// regenerating islet-derived 1 beta (pancreatic stone protein, pancreatic thread protein)

1165 209820_ _s_at TBL3 transduciπ (beta)-like 3 BC002361 gl 2803114 NP 006444 Chr:16p13.3 0.22647 1.2231891165 209820_ _s_at TBL3 transduciπ (beta) -like 3 BC002361 gl 2803114 NP 006444 Chr: 16p13.3 0.22647 1.223189

1166 201909_ _at RPS4Y1 ribosoma! protein S4, NM_001008 g4506726 NP. ,000999 Chr:yp11.3 0.22634 0.095261 Y-linked 1 /// ribosomal protein S4, Y-linked 11166 201909_ _at RPS4Y1 ribosoma! protein S4, NM_001008 g4506726 NP. , 000999 Chr: yp11.3 0.22634 0.095261 Y-linked 1 /// ribosomal protein S4, Y-linked 1

1167 206262 at ADH1C alcohol dehydrogenase NM_000669 gl 1496888 NP_000660 Chr:4q21-q23 0.22627 3.612633 1 C (class I), gamma Polypeptide /// alcohol dehydrogenase 1 C (class I), gamma Polypeptide1167 206262 at ADH1C alcohol dehydrogenase NM_000669 gl 1496888 NP_000660 Chr: 4q21-q23 0.22627 3.612633 1 C (class I), gamma polypeptides /// alcohol dehydrogenase 1 C (class I), gamma polypeptides

1168 229729. _at C9orf127 chromosome 9 open BF507870 Hs.143669.0 NP_057530 Chr:9p13.1 0.22607 0.353669 reading frame 1271168 229729. _at C9orf127 chromosome 9 open BF507870 Hs.143669.0 NP_057530 Chr: 9p13.1 0.22607 0.353669 reading frame 127

1169 232623. _at INM04 mRNA, AK024541 Hs.191945.0 — — 0.22585 0.095261 complete cds1169 232623. _at INM04 mRNA, AK024541 Hs.191945.0 - - 0.22585 0.095261 complete cds

1170 213661_ .at DKFZP586H2123 DKFZP586H2123 AI671186 Hs.55044.0 NP_056245 Chr:11p13 0.22568 8.779357 protein1170 213661_ .at DKFZP586H2123 DKFZP586H2123 AI671186 Hs.55044.0 NP_056245 Chr: 11p13 0.22568 8.779357 protein

1171 229687. _s_at CDNA FLJ14242 fis, AA621763 Hs.170434.0 — — 0.22407 0.779487 clone OVARCI 000678 1171 229687. _s_at CDNA FLJ14242 fis, AA621763 Hs.170434.0 - - 0.22407 0.779487 clone OVARCI 000678

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer JTJ Protein ID Lokalisierung Change (%)Sample Number JTJ Protein ID Localization Change (%)

1172 219019 at LRDD ieucine-rich repeats NM_018494 g8923912 NP_060964 Chr:11p15.5 0.22295 1.051298 and death domain containing /// Ieucine- rich repeats and death domain containinc1172 219019 at LRDD ieucine-rich repeats NM_018494 g8923912 NP_060964 Chr: 11p15.5 0.22295 1.051298 and death domain containing /// Ieucine- repeats and death domain containinc

1173 208147 s at CYP2C8 cytochrome P450, NM_030878 gl 3787186 NP_000761 Chr:10q24.1 0.21996 6.991358 family 2, subfamily C, Polypeptide 8 /// cytochrome P450, family 2, subfamily C, Polypeptide 8 /// cytochrome P450, family 2, subfamily C, Polypeptide 81173 208147 s at CYP2C8 cytochrome P450, NM_030878 gl 3787186 NP_000761 Chr: 10q24.1 0.21996 6.991358 family 2, subfamily C, polypeptides 8 /// cytochrome P450, family 2, subfamily C, polypeptides 8 /// cytochrome P450, family 2, subfamily C, polypeptides 8

1174 205923_ _at RELN reelin /// reelin NM_005045 g4826977 NP _005036 Chr:7q22 0.2191 2.5713221174 205923_ _at RELN reelin /// reelin NM_005045 g4826977 NP _005036 Chr: 7q22 0.2191 2.571322

1175 237251. _at FLJ32884 hypothetical protein AI480252 Hs.137368.0 NP. _653303 Chr:1q23.1 0.21871 0.095261 FLJ328841175 237251 . _at FLJ32884 hypothetical protein AI480252 Hs.137368.0 NP. _653303 Chr: 1q23.1 0.21871 0.095261 FLJ32884

1176 201849 at BNIP3 BCL2/adenovirus E1 B NM_004052 g7669480 NP_004043 Chr:10q26.3 0.21788 0.095261 κ> 19kDa interacting protein 3 /// BCL2/adenovirus E1B 19kDa interacting protein 31176 201849 at BNIP3 BCL2 / adenovirus E1B NM_004052 g7669480 NP_004043 Chr: 10q26.3 0.21788 0.095261 κ> 19kDa interacting protein 3 /// BCL2 / adenovirus E1B 19kDa interacting protein 3

1177 212592. _at AV733266 Hs.76325.1 0.21763 0.0952611177 212592 . _at AV733266 Hs.76325.1 0.21763 0.095261

1178 228492. _at USP9Y ubiquitin specific AV681765 Hs.1870.1 NPJ304645 Chr:Yq11.2 0.21384 0.095261 Protease 9, Y-linked (fat facets-like, Drosophila)1178 228492. _at USP9Y ubiquitin specific AV681765 Hs.1870.1 NPJ304645 Chr: Yq11.2 0.21384 0.095261 Protease 9, Y-linked (fat facets-like, Drosophila)

1179 227826. _s_at Clone AW138143 Hs.71721.0 0.2128 2.814919 IMAGE:4791597, mRNA1179 227826. _s_at Clone AW138143 Hs.71721.0 0.2128 2.814919 IMAGE: 4791597, mRNA

1180 232765. _x_at Hs.272320.0 0.21117 0.0952611180 232765. _x_at Hs.272320.0 0.21117 0.095261

1181 211712. _s_at ANXA9 annexin A9 /// annexin BC005830 gl 3543331 NP_003559 Chr:1q21 0.21102 0.095261 A9 1181 211712. _s_at ANXA9 annexin A9 /// annexin BC005830 gl 3543331 NP_003559 Chr: 1q21 0.21102 0.095261 A9

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung ChangeSample ID ID Protein ID Localization Change

1182 205799 s at SLC3A1 solute carrier family 3 M95548 g306441 NP_000332 Chr:2p16.3 0.20871 3.6126331182 205799 s at SLC3A1 solute carrier family 3 M95548 g306441 NP_000332 Chr: 2p16.3 0.20871 3.612633

(cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1 /// solute carrier family 3(cystine, dibasic and neutral amino acid transporter, activator of cystine, dibasic and neutral amino acid transport), member 1 /// solute carrier family 3

(cystine, dibasic and neutral amino acid transporters, activator of cystin(cystine, dibasic and neutral amino acid transporter, activator of cystine

1183 204437 s at FOLR1 folate receptor 1 (adult) NML.016725 g9257206 NP_000793 Chr:11q13.3- 0.20709 10.20301 /// folate receptor 1 q14.1 (adult)1183 204437 s at FOLR1 folate receptor 1 (adult) NML.016725 g9257206 NP_000793 Chr: 11q13.3- 0.20709 10.20301 /// folate receptor 1 q14.1 (adult)

1184 208473 s at GP2 glycoprotein 2 NM_016295 g7706490 NP_001493 Chr:9q21.11- 0.20347 1.380569 (zymogen granule q21.2 membrane) /// glycoprotein 2 (zymogen granule membrane)1184 208473 s at GP2 glycoprotein 2 NM_016295 g7706490 NP_001493 Chr: 9q21.11- 0.20347 1.380569 (zymogen granule q21.2 membrane) /// glycoprotein 2 (zymogen granule membrane)

1185 210497_x_at SSX2 synovial sarcoma, X BC002818 gl 2803942 NP. _003138 Chr:xp11.23- 0.2017 0.095261 breakpoint 2 P11.221185 210497_x_at SSX2 synovial sarcoma, X BC002818 gl 2803942 NP . _003138 Chr: xp11.23-0.2017 0.095261 breakpoint 2 P11.22

1186 222717_at SDPR serum deprivation BF982174 Hs.26530.0 NP. ,004648 Chr:2q32-q33 0.20109 0.353669 response (phosphatidylserine binding protein)1186 222717_at SDPR serum deprivation BF982174 Hs.26530.0 NP . , 004648 Chr: 2q32-q33 0.20109 0.353669 response (phosphatidylserine binding protein)

1187 219064_at ITIH5 inter-alpha (globulin) NM_030569 gl 3386477 NP. _085046 Chr:10p15.1 0.19975 0.095261 inhibitor H51187 219064_at ITIH5 inter-alpha (globulin) NM_030569 gl 3386477 NP. _085046 Chr: 10p15.1 0.19975 0.095261 inhibitor H5

1188 244223 at — Transcribed sequences AW207574 Hs.179501.0 — — 0.19883 0.7794871188 244223 at - Transcribed sequences AW207574 Hs.179501.0 - - 0.19883 0.779487

1189 231584_s_at BCAS4 breast Carcinoma BE503623 Hs.56237.0 NP. ,060313 Chr:20q13.13 0.1973 9.266777 amplified sequence 41189 231584_s_at BCAS4 breast carcinoma BE503623 Hs.56237.0 NP. , 060313 Chr: 20q13.13 0.1973 9.266777 amplified sequence 4

1190 242400_at ZFP90 zinc finger protein 90 AI885183 Hs.173552.0 — Chr:16q22.1 0.19714 0.201325 homolog (mouse)1190 242400_at ZFP90 zinc finger protein 90 AI885183 Hs.173552.0 - Chr: 16q22.1 0.19714 0.201325 homolog (mouse)

1191 240242_at — Transcribed sequences BE222843 Hs.124590.0 — — 0.19628 9.266777 1191 240242_at - Transcribed sequences BE222843 Hs.124590.0 - - 0.19628 9.266777

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change <0'-Sample ID ID Protein ID Localization Change < 0 '-

1192 227450 at FLJ32115 hypothetical protein AI051248 Hs.293902.0 NP_689534 Chr:12p13.1 0.19621 9.266777 FLJ321151192 227450 at FLJ32115 hypothetical protein AI051248 Hs.293902.0 NP_689534 Chr: 12p13.1 0.19621 9.266777 FLJ32115

1193 208383 s at PCK1 phosphoenolpyruvate NM_002591 g4505638 NP_002582 Chr:20q13.31 0.19527 0.149911 carboxykinase 1 (soluble) /// phosphoenolpyruvate carboxykinase 1 (soluble)1193 208383 s at PCK1 phosphoenolpyruvate NM_002591 g4505638 NP_002582 Chr: 20q13.31 0.19527 0.149911 carboxykinase 1 (soluble) /// phosphoenolpyruvate carboxykinase 1 (soluble)

1194 209266_s_at SLC39A8 solute carrier family 39 AW134794 Hs.284205.1 NP_071437 Chr:4q22-q24 0.19436 0.095261 (zinc transporter), member 81194 209266_s_at SLC39A8 solute carrier family 39 AW134794 Hs.284205.1 NP_071437 Chr: 4q22-q24 0.19436 0.095261 (zinc transporter), member 8

1195 223316_at gl 3276630 0.19423 0.0952611195 223316_at gl 3276630 0.19423 0.095261

1196 229963_at LOC340542 hypothetical protein AV726956 Hs.47209.0 — Chr:xq22.1 0.19287 4.75532 LOC3405421196 229963_at LOC340542 hypothetical protein AV726956 Hs.47209.0 - Chr: xq22.1 0.19287 4.75532 LOC340542

1197 238357_at — Transcribed sequences AW749121 Hs.282901.0 — — 0.19232 0.0952611197 238357_at - Transcribed sequences AW749121 Hs.282901.0 - - 0.19232 0.095261

1198 205912_at PNLIP pancreatic lipase /// NM_000936 gl 0834999 NP_000927 Chr:10q26.1 0.19063 3.612633 pancreatic lipase1198 205912_at PNLIP pancreatic lipase /// NM_000936 gl 0834999 NP_000927 Chr: 10q26.1 0.19063 3.612633 pancreatic lipase

1199 223629_at PCDHB5 protocadherin beta 5 BC001186 gl 2654692 NP_056484 Chr:5q31 0.19043 0.095261 4-1199 223629_at PCDHB5 protocadherin beta 5 BC001186 gl 2654692 NP_056484 Chr: 5q31 0.19043 0.095261 4-

1200 213907_at EEF1 E1 eukaryotic translation N32257 Hs.298581.1 NP_004271 Chr:6p24.3- 0.18975 0.095261 elongation factor 1 p25.1 epsilon 11200 213907_at EEF1 E1 eukaryotic translation N32257 Hs.298581.1 NP_004271 Chr: 6p24.3- 0.18975 0.095261 elongation factor 1 p25.1 epsilon 1

1201 211766_s_at PNLIPRP2 pancreatic lipase- BC005989 gl 3543678 NP_005387 Chr:10q26.12 0.18805 3.612633 related protein 2 /// pancreatic lipase- related protein 21201 211766_s_at PNLIPRP2 pancreatic lipase BC005989 gl 3543678 NP_005387 Chr: 10q26.12 0.18805 3.612633 related protein 2 /// pancreatic lipase-related protein 2

1202 205509_at CPB1 carboxypeptidase B1 NM_001871 g4503002 NP_001862 Chr:3q24 0.18525 4.436744 (tissue) /// carboxypeptidase B1 (tissue)1202 205509_at CPB1 carboxypeptidase B1 NM_001871 g4503002 NP_001862 Chr: 3q24 0.18525 4.436744 (tissue) /// carboxypeptidase B1 (tissue)

1203 221796_at NTRK2 neurotrophic tyrosine AA707199 Hs.298998.1 NP_006171 Chr:9q22.1 0.18166 0.149911 kinase, receptor, type 2 1203 221796_at NTRK2 neurotrophic tyrosine AA707199 Hs.298998.1 NP_006171 Chr: 9q22.1 0.18166 0.149911 kinase, receptor, type 2

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript (O Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript (O Representative Chromosomal FoId q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

1204 203638 s at FGFR2 fibroblast growth factor NM_022969 g13186252 NP_000132 Chr:10q26 0.18165 6.991358 receptor 2 (bacteria- expressed kiπase, keratinocyte growth factor receptor, craniofacial dysostosis 1 , Crouzon Syndrome, Pfeiffer Syndrome, Jackson-Weiss Syndrome)1204 203638 s at FGFR2 fibroblast growth factor NM_022969 g13186252 NP_000132 Chr: 10q26 0.18165 6.991358 receptor 2 (bacteria-expressed kiπase, keratinocyte growth factor receptor, craniofacial dysostosis 1, Crouzon syndrome, Pfeiffer syndrome, Jackson-Weiss syndrome)

1205 206700 s at JARID1D Jumonji, AT rieh NM_004653 g4759149 NP_004644 Chr:yq11 0.17638 0.095261 interactive domain 1 D (RBP2-Iike) /// Jumonji, AT rieh interactive domain 1 D (RBP2-Iike)1205 206700 s at JARID1D Jumonji, AT rieh NM_004653 g4759149 NP_004644 Chr: yq11 0.17638 0.095261 interactive domain 1 D (RBP2-Iike) /// Jumonji, AT rieh interactive domain 1 D (RBP2-Iike)

1206 205771 s at AKAP7 A kinase (PRKA) AL137063 Hs.12835.0 NP 004833 Chr:6q23 0.1757 3.612633 anchor protein 71206 205771 s at AKAP7 A kinase (PRKA) AL137063 Hs.12835.0 NP 004833 B: 6q23 0.1757 3.612633 anchor protein 7

1207 209752 at REG1A regenerating islet- AF172331 g5764554 NP_002900 . Chr:2p12 0.1729 0.095261 derived 1 alpha (pancreatic stone protein, pancreatic thread protein) /// regenerating islet- derived 1 alpha (pancreatic stone protein, pancreatic thread protein)1207 209752 at REG1A regenerating islet- AF172331 g5764554 NP_002900. Chr: 2p12 0.1729 0.095261 derived 1 alpha (pancreatic stone protein, pancreatic thread protein) /// regenerating islet-derived 1 alpha (pancreatic stone protein, pancreatic thread protein)

1208 223843. _at SCARA3 seavenger receptor AB007830 g6230378 NP. .057324 Chr:8p21 0.17173 0.095261 dass A, member 31208 223843. _at SCARA3 seavenger receptor AB007830 g6230378 NP. .057324 Chr: 8p21 0.17173 0.095261 that A, member 3

1209 209261. _s_ at NR2F6 nuclear receptor BF000629 Hs.239752.0 NP. .005225 Chr:19p13.1 0.16835 0.518167 subfamily 2, group F, member 61209 209261. _s_ at NR2F6 nuclear receptor BF000629 Hs.239752.0 NP. .005225 Chr: 19p13.1 0.16835 0.518167 subfamily 2, group F, member 6

1210 208498. A_ g4757749 0.16694 3.612633 1210 208498. A_ g4757749 0.16694 3.612633

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoW q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoW q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

1211 204081 at NRGN neurogranin (protein NM_006176 g5453799 NP_006167 Chr:11q24 0.16629 0.095261 kinase C Substrate, RC3) /// neurogranin (protein kinase C Substrate, RC3)1211 204081 at NRGN neurogranin (protein NM_006176 g5453799 NP_006167 Chr: 11q24 0.16629 0.095261 kinase C substrates, RC3) /// neurogranin (protein kinase C substrates, RC3)

1212 215624 at TSC2 tuberous sclerosis 2 AU147962 Hs.301509.0 NP 000539 Chr:16p13.3 0.16367 11.702041212 215624 at TSC2 tuberous sclerosis 2 AU147962 Hs.301509.0 NP 000539 Chr: 16p13.3 0.16367 11.70204

1213 219270_at MGC4504 hypothetical protein NM_024111 gl 3129125 NP. „077016 Chr:15q14 0.16307 1.380569 MGC45041213 219270_at MGC4504 hypothetical protein NM_024111 gl 3129125 NP. " 077016 Chr: 15q14 0.16307 1.380569 MGC4504

1214 231037_at — CDNA FLJ26031 fis, AA004300 Hs.166254.1 — 0.16295 6.023405 clone PNC080781214 231037_at - CDNA FLJ26031 fis, AA004300 Hs.166254.1 - 0.16295 6.023405 clone PNC08078

1215 211738_x_at ELA3A elastase 3A, pancreatic BC005918 gl 3543517 NP. _005738 Chr:1p36.12 0.15996 3.612633 (Protease E) /// elastase 3A, pancreatic (Protease E)1215 211738_x_at ELA3A elastase 3A, pancreatic BC005918 gl 3543517 NP. _005738 Chr: 1p36.12 0.15996 3.612633 (Protease E) /// elastase 3A, pancreatic (Protease E)

1216 233514_x_at TEX11 testis expressed AL139109 Hs.121776.1 NP_ J 12566 Chr:xq13.1 0.15817 9.266777 sequence 111216 233514_x_at TEX11 testis expressed AL139109 Hs.121776.1 NP_ J 12566 Chr: xq13.1 0.15817 9.266777 sequence 11

1217 206535 at SLC2A2 solute carrier family 2 NM 000340 g4557850 NP 000331 Chr:3q26.1- 0.15383 6.991358 (facilitated glucose q26.2 transporter), member 2 /// solute carrier family 2 (facilitated glucose transporter), member 21217 206535 at SLC2A2 solute carrier family 2MM 000340 g4557850 NP 000331Cr: 3q26.1- 0.15383 6.991358 (facilitated glucose q26.2 transporter), member 2 /// solute carrier family 2 (facilitated glucose transporter), member 2

1218 205477 s at AMBP alpha-1- NM_001633 g4502066 NP_001624 Chr:9q32-q33 0.15063 0.353669 microglobulin/bikunin precursor /// alpha-1 - microglobulin/bikunin precursor1218 205477 s at AMBP alpha-1-NM_001633 g4502066 NP_001624 Chr: 9q32-q33 0.15063 0.353669 microglobulin / bikunin precursor /// alpha-1-microglobulin / bikunin precursor

1219 206784, _at AQP8 aquaporin 8 /// NM_001169 g4502188 NP_001160 Chr:16p12 0.14934 2.814919 aquaporin 81219 206784 , _at AQP8 aquaporin 8 /// NM_001169 g4502188 NP_001160 Chr: 16p12 0.14934 2.814919 aquaporin 8

1220 223623, _at ECRG4 esophageal cancer AF325503 gl 1991655 NP_115787 Chr:2q12.2 0.14859 0.095261 related gene 4 protein1220 223623 , _at ECRG4 esophageal cancer AF325503 gl 1991655 NP_115787 Chr: 2q12.2 0.14859 0.095261 related gene 4 protein

1221 215563_ _s_at MSTP9 macrophage U28055 Hs.278657.3 — Chr:1p36.13 0.14797 4.149813 stimulattng,

Figure imgf000097_0001
1221 215563__s_at MSTP9 macrophage U28055 Hs.278657.3 - Chr: 1p36.13 0.14797 4.149813 stimulattng,
Figure imgf000097_0001

Nr. Affyimetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affyimetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1222 222722_at OGN osteoglycin AV700059 Hs.109439.0 NP_054776 Chr:9q22 0.14778 0.095261 (osteoinductive factor, mimecan)1222 222722_at OGN osteoglycin AV700059 Hs.109439.0 NP_054776 Chr: 9q22 0.14778 0.095261 (osteoinductive factor, mimecan)

1223 206681_x_at NM_001502 g4504074 0.1472 0.0952611223 206681_x_at NM_001502 g4504074 0.1472 0.095261

1224 235737_at TSLP thymic stromal AW118681 Hs.128051.0 NP_149024 Chr:5q22.2 0.14708 0.095261 lymphopoietin1224 235737_at TSLP thymic stromal AW118681 Hs.128051.0 NP_149024 Chr: 5q22.2 0.14708 0.095261 lymphopoietin

1225 221066 at g7706102 0.14576 0.0952611225 221066 at g7706102 0.14576 0.095261

1226 210262_at CRISP2 cysteiπe-rich secretory M25532 g339882 NP_003287 Chr:6p21-qter 0.14408 4.436744 protein 2 /// cysteine- rich secretory protein 21226 210262_at CRISP2 cysteine secretory M25532 g339882 NP_003287 Chr: 6p21-qter 0.14408 4.436744 protein 2 /// cysteine-rich secretory protein 2

1227 209277_at TFP12 tissue factor pathway AL574096 Hs.295944.0 NP_006519 Chr:7q22 0.13882 0.095261 Inhibitor 21227 209277_at TFP12 tissue factor pathway AL574096 Hs.295944.0 NP_006519 Chr: 7q22 0.13882 0.095261 Inhibitor 2

1228 219564 at KCNJ16 potassium inwardly- NMJ318658 g8923822 NP_061128 Chr:17q23.1- 0.13757 3.612633 rectifying Channel, q24.2 subfamily J, member 16 /// potassium inwardly-rectifying Channel, subfamily J, member 161228 219564 at KCNJ16 potassium inwardly- NMJ318658 g8923822 NP_061128 Chr: 17q23.1- 0.13757 3.612633 rectifying channel, q24.2 subfamily J, member 16 /// potassium in-the-wall, subfamily J, member 16

1229 205000 at DDX3Y DEAD (Asp-Glu-Ala- NM_004660 gl 3514808 NP_004651 Chr:yq11 0.13637 0.095261 Asp) box Polypeptide 3, Y-Iinked /// DEAD (Asp- Glu-Ala-Asp) box Polypeptide 3, Y-Iinked1229 205000 at DDX3Y DEAD (Asp-Glu-Ala-NM_004660 gl 3514808 NP_004651 Chr: yq11 0.13637 0.095261 Asp) box polypeptides 3, Y-linked /// DEAD (Asp-Glu-Ala-Asp) box polypeptides 3, Y-linked

1230 224400 s at CHST9 carbohydrate (N- AF332473 gl 3561519 NP_113610 Chr:18q11.2 0.13533 0.095261 acetylgalactosamine 4- 0) sulfotransferase 9 /// carbohydrate (N- acetylgalactosamine 4- Q) sulfotransferase 91230 224400 s at CHST9 carbohydrate (N-AF332473 gl 3561519 NP_113610 Chr: 18q11.2 0.13533 0.095261 acetylgalactosamine 4-0) sulfotransferase 9 /// carbohydrate (N-acetylgalactosamine 4-Q) sulfotransferase 9

1231 230736 at SVIL supervillin AW118878 Hs.110835.0 NP. 003165 Chr:10p11.2 0.13164 0.0952611231 230736 at SVIL supervillin AW118878 Hs.110835.0 NP . 003165 Chr: 10p11.2 0.13164 0.095261

1232 238018_at LOC285016 hypothetical protein BF449053 Hs.213923.0 — Chr:2p25.3 0.13038 0.095261 LOC285016Hypothetical protein BF449053 Hs.213923.0 - Chr: 2p25.3 0.13038 0.095261 LOC285016

1233 231773 at ANGPTL1 angiopoietin-like 1 BF002046 Hs.241519.0 NP 004664 Chr:1q24.3 0.12641 0.0952611233 231773 at ANGPTL1 angiopoietin-like 1 BF002046 Hs.241519.0 NP 004664 Chr: 1q24.3 0.12641 0.095261

1234 240259_at — LOC400235 AI188161 Hs.144627.0 — Chr:14q31.3 0.12466 9.840216 (LOC388003), mRNA 1234 240259_at - LOC400235 AI188161 Hs.144627.0 - Chr: 14q31.3 0.12466 9.840216 (LOC388003), mRNA

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1235 213071 at DPT dermatopontin AL049798 Hs.80552.1 NP 001928 Chr:1q12-q23 0.12167 3.6126331235 213071 at DPT dermatopontin AL049798 Hs.80552.1 NP 001928 Chr: 1q12-q23 0.12167 3.612633

1236 234316_x_at KLK12 kallikrein 12 AF135025 Hs.159679.1 NPJD62544 Chr:19q13.3- 0.12155 6.023405 q13.41236 234316_x_at KLK12 kallikrein 12 AF135025 Hs.159679.1 NPJD62544 Chr: 19q13.3- 0.12155 6.023405 q13.4

1237 235915 at Transcribed sequence R88990 Hs.34226.0 0.12054 6.023405 with weak similarity to protein ref:NP_004563.1 (H.sapiens) plakophilin 2 [Homo sapiens]1237 235915 at Transcribed sequence R88990 Hs.34226.0 0.12054 6.023405 with weak similarity to protein ref: NP_004563.1 (H.sapiens) plakophilin 2 [Homo sapiens]

1238 207778 at REGL regenerating islet- NM_006508 gl 0835249 NP_006499 Chr:2p12 0.11887 0.095261 derived-like, pancreatic stone protein-like, pancreatic thread protein-like (rat) /// regenerating islet- derived-like, pancreatic stone protein-like, pancreatic thread protein-like (rat)1238 207778 at REGL regenerating islet NM_006508 gl 0835249 NP_006499 Chr: 2p12 0.11887 0.095261 derived-like, pancreatic stone protein-like, pancreatic thread protein-like (rat) /// regenerating islet-derived-like, pancreatic stone protein-like, pancreatic thread protein-like (rat)

1239 214731_at DKFZp547A023 hypothetical protein AB037854 Hs.23921.1 NP_061174 Chr:1p13.2 0.11625 0.095261 DKFZp547A0231239 214731_at DKFZp547A023 hypothetical protein AB037854 Hs.23921.1 NP_061174 Chr: 1p13.2 0.11625 0.095261 DKFZp547A023

1240 230432_at — CDNA FLJ42409 fis, AI733124 Hs.14535.0 — 0.11211 9.266777 clone BLADE20008661240 230432_at - CDNA FLJ42409 fis, AI733124 Hs.14535.0 - 0.11211 9.266777 clone BLADE2000866

1241 206212_at CPA2 carboxypeptidase A2 NM_001869 g4502998 NP_001860 Chr:7q32 0.11123 4.149813 (pancreatic) /// carboxypeptidase A2 (pancreatic)1241 206212_at CPA2 carboxypeptidase A2 NM_001869 g4502998 NP_001860 Chr: 7q32 0.11123 4.149813 (pancreatic) /// carboxypeptidase A2 (pancreatic)

1242 215327_at — Transcribed sequences AI675458 Hs.206984.0 — — 0.10593 0.0952611242 215327_at - Transcribed sequences AI675458 Hs.206984.0 - - 0.10593 0.095261

1243 216597_at TRY2 trypsinogen-like U66059 Hs.303157.12 — Chr:7q34 0.10405 1.223189 pseudogene 1243 216597_at TRY2 trypsinogen-like U66059 Hs.303157.12 - Chr: 7q34 0.10405 1.223189 pseudogene

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change ""-Sample Number ID Protein ID Localization Change "" -

1244 217452 s at B3GALT2 UDP-GaI rbetaGIcNAc Y15014 Hs.181353.1 NP_003774 Chr:1q31 0.10384 4.436744 bete 1,3- gaiactosyltransferase, Polypeptide 2 /// UDP- GahbetaGIcNAc beta 1.3- galactosyltransferase, Polypeptide 21244 217452 s at B3GALT2 UDP-GaI rbetaGIcNAc Y15014 Hs.181353.1 NP_003774 Chr: 1q31 0.10384 4.436744 bee 1,3-gaiactosyltransferase, polypeptides 2 /// UDP-GahbetaGlcNAc beta 1,3-galactosyltransferase, polypeptides 2

1245 235965 at BF513674 Hs.22627.0 0.10241 0.0952611245 235965 at BF513674 Hs.22627.0 0.10241 0.095261

1246 220275 at CUZD1 CUB and zona NM_022034 g11545736 NP_071317 Chr:10q26.13 0.10068 3.612633 pellucida-like domains 1 /// CUB and zona pellucida-like domains 11246 220275 at CUZD1 CUB and zone NM_022034 g11545736 NP_071317 Chr: 10q26.13 0.10068 3.612633 pellucida-like domains 1 /// CUB and zona pellucida-like domains 1

1247 213967_at LOC138046 hypothetical protein AI634532 Hs.13423.0 NP. _776247 Chr:8q21.13 0.09925 0.095261 LOC1380461247 213967_at LOC138046 hypothetical protein AI634532 Hs.13423.0 NP . _776247 Chr: 8q21.13 0.09925 0.095261 LOC138046

1248 206464_at BMX BMX non-receptor NM_001721 g4502434 NP_ _001712 Chr:xp22.2 0.09884 3.612633 tyrosine kinase /// BMX non-receptor tyrosine kinase1248 206464_at BMX BMX non-receptor NM_001721 g4502434 NP_ _001712 Chr: xp22.2 0.09884 3.612633 tyrosine kinase /// BMX non-receptor tyrosine kinase

1249 225016_at APCDD1 adenomatosis N48299 Hs.20665.0 NP. _694545 Chr:18p11.21 0.0986 4.75532 Polyposis coli down- regulated 11249 225016_at APCDD1 adenomatosis N48299 Hs.20665.0 NP . _694545 Chr: 18p11.21 0.0986 4.75532 Polyposis coli down-regulated 1

1250 207985_at ENO1B enolase alpha, lung- NM_014355 g7657061 0.09814 0.625663 specific /// enolase alpha, lung-specific1250 207985_at ENO1B enolase alpha, lung NM_014355 g7657061 0.09814 0.625663 specific // enolase alpha, lung-specific

1251 240578 at — Transcribed sequences BF435290 Hs.132247.0 — — 0.09355 0.0952611251 240578 at - Transcribed sequences BF435290 Hs.132247.0 - - 0.09355 0.095261

1252 206297_at CTRC chymotrypsin C NM_007272 gl 1321627 NP. ,009203 Chr:1 p36.13 0.09328 3.61384 (caldecrin) /// chymotrypsin C (caldecrin)1252 206297_at CTRC chymotrypsin C NM_007272 gl 1321627 NP . , 009203 Chr: 1 p36.13 0.09328 3.61384 (caldecrin) /// chymotrypsin C (caldecrin)

1253 216687_x_at UGT2B15 UDP U06641 Hs.150207.1 NP. _001067 Chr:4q13 0.08879 1.578435 glycosyltransferase 2 family, Polypeptide B151253 216687_x_at UGT2B15 UDP U06641 Hs.150207.1 NP . _001067 Chr: 4q13 0.08879 1.578435 glycosyltransferase 2 family, polypeptides B15

1254 236430_at MGC23911 hypothetical protein AA708152 Hs.119840.0 NP. ,653277 Chr:16q22.1 0.08763 0.095261 MGC23911 1254 236430_at MGC23911 hypothetical protein AA708152 Hs.119840.0 NP . , 653277 Chr: 16q22.1 0.08763 0.095261 MGC23911

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1255 229995 at SYCN syncollin BE969669 Hs.124123.0 — Chr:19q13.2 0.08642 0.2013251255 229995 at SYCN syncollin BE969669 Hs.124123.0 - Chr: 19q13.2 0.08642 0.201325

1256 41512_at BRAP BRCA1 associated AL042733 4921950_RC NP_006759 Chr:12q24 0.08093 6.991358 protein1256 41512_at BRAP BRCA1 associated with AL042733 4921950_RC NP_006759 Chr: 12q24 0.08093 6.991358 protein

1257 220487_at SNTG2 syntrophin, gamma 2 /// NM_018968 g9507164 NP_061841 Chr:2p25 0.08015 0.095261 syntrophin, gamma 21257 220487_at SNTG2 syntrophin, gamma 2 /// NM_018968 g9507164 NP_061841 Chr: 2p25 0.08015 0.095261 syntrophin, gamma 2

1258 205719_s_at PAH Phenylalanine NM_000277 g4557818 NP_000268 Chr:12q22- 0.07897 1.578435 hydroxylase /// q24.2 Phenylalanine hydroxylase1258 205719_s_at PAH Phenylalanine NM_000277 g4557818 NP_000268 Chr: 12q22- 0.07897 1.578435 hydroxylase /// q24.2 Phenylalanine hydroxylase

1259 228004_at C20orf56 chromosome 20 open AL121722 Hs.9842.0 — Chr:20p11.22 0.07039 1.223189 reading frame 561259 228004_at C20orf56 chromosomes 20 open AL121722 Hs.9842.0 - Chr: 20p11.22 0.07039 1.223189 reading frame 56

1260 229975_at — Transcribed sequences AI826437 Hs.72472.0 — — 0.06921 0.0952611260 229975_at - Transcribed sequences AI826437 Hs.72472.0 - - 0.06921 0.095261

1261 209616 s at CES1 carboxylesterase 1 S73751 g688112 NP 001257 Chr:16q13- 0.06796 8.779357 (monocyte/macrophage q22.1 serine esterase 1 ) /// carboxylesterase 1 (monocyte/macrophage serine esterase 1 )1261 209616 s at CES1 carboxylesterase 1 S73751 g688112 NP 001257 Chr: 16q13- 0.06796 8.779357 (monocyte / macrophage q22.1 serine esterase 1) /// carboxylesterase 1 (monocyte / macrophage serine esterase 1)

1262 224970. _at NFIA nuclear factor l/A AA419275 Hs.173933.0 NP. _005586 Chr:1 p31.3- 0.06238 0.095261 P31.21262 224970. _at NFIA nuclear factor I / A AA419275 Hs.173933.0 NP . _005586 Chr: 1 p31.3- 0.06238 0.095261 P31.2

1263 229778_ _at MGC10946 hypothetical protein BE326710 Hs.121773.0 NP. ,085049 Chr:12p12.3 0.05486 0.353669 MGC109461263 229778_ _at MGC10946 hypothetical protein BE326710 Hs.121773.0 NP . , 085049 Chr: 12p12.3 0.05486 0.353669 MGC10946

1264 241390 at — Transcribed sequences AW117264 Hs.126252.0 — — 0.04071 0.1499111264 241390 at - Transcribed sequences AW117264 Hs.126252.0 - - 0.04071 0.149911

1265 206827 s at TRPV6 transient receptor NMJD14274 g7656846 NP_061116 Chr:7q33-q34 0.04018 3.612633 Potential cation Channel, subfamily V, member 6 /// transient receptor potential cation Channel, subfamily V, member 61265 206827 s at TRPV6 transient receptor NMJD14274 g7656846 NP_061116 Chr: 7q33-q34 0.04018 3.612633 Potential cation channel, subfamily V, member 6 /// transient receptor potential cation Channel, subfamily V, member 6

1266 214925. _s_at SPTAN 1 spectrin, alpha, non- AK026484 Hs.321666.0 NP. _003118 Chr:9q33-q34 0.03631 0.779487 erythrocytic 1 (alpha- fodrin)1266 214925. _s_at SPTAN 1 spectrin, alpha, non-AK026484 Hs.321666.0 NP . _003118 Chr: 9q33-q34 0.03631 0.779487 erythrocytic 1 (alpha fodrin)

1267 229177. _at MGC45438 hypothetical protein AI823572 Hs.11782.0 NP. ,689672 Chr:16p13.3 0.03437 0.353669 MGC45438 1267 229177 . _at MGC45438 hypothetical protein AI823572 Hs.11782.0 NP. , 689672 Chr: 16p13.3 0.03437 0.353669 MGC45438

Die Probe biologischen Materials, die zur Diagnose des Pankreaskarzinoms einem Patienten entnommen wird, ist eine Gewebe- oder Blut- oder Serumprobe, die Proteine und/oder Nukleinsäuren enthält. Bevorzugt enthält die Probe Pankreasgewebe, das durch während der operativen Entfernung oder einer Feinnadelbiopsie oder einer ERCP einem Patienten entnommen wird. Das entnommene Gewebe wird in flüssigen Stickstoff eingefroren oder in andere Flüssigkeiten z.B: RNAIater eingebracht, welche die Integrität der RNA, der DNA und der Proteine gewährleistet. Alsdann werden von dem gefrorenen Gewebeblock in einem Kryostaten einzelne Schnitte mit der große von ca. 10 μm hergestellt. Diese Schnitte werden in Ethanol fixiert und mit Hematoxilin angefärbt. Unter dem Mikrospkop wird dann mittels eines spitzen Gegenstandes oder eines Laserstrahls das entsprechende interessierende Gewebe herausgelöst und in ein Behältnis gegeben. Dazu wird eine Flüssigkeit hinzugefügt, welche die in dem Gewebe enthaltene RNA, DNS, oder Protein- Moleküle konserviert.The sample of biological material taken from a patient for diagnosis of pancreatic carcinoma is a tissue or blood or serum sample containing proteins and / or nucleic acids. Preferably, the sample contains pancreatic tissue taken from a patient during surgical removal or fine needle biopsy or ERCP. The harvested tissue is frozen in liquid nitrogen or introduced into other fluids, such as RNAIater, which ensure the integrity of the RNA, DNA and proteins. Then, of the frozen tissue block in a cryostat individual sections are made with the large of about 10 microns. These sections are fixed in ethanol and stained with hematoxylin. Under the Mikrospkop then the corresponding tissue of interest is removed by means of a sharp object or a laser beam and placed in a container. For this purpose, a liquid is added which preserves the RNA, DNA, or protein molecules contained in the tissue.

Alternativ erfolgt die Bestimmung in einer Blutprobe oder Serum oder Plasma, das aus dem Blut des Patienten gewonnen wurde. Desweiteren erfolgt die Bestimmung in einer Probe des Stuhls, der Gallengangs- oder der Pankreasflüssigkeit die aus dem Patienten gewonnen wurde.Alternatively, the determination is made in a blood sample or serum or plasma obtained from the patient's blood. Furthermore, the determination is carried out in a sample of the stool, the bile duct or the pancreatic fluid obtained from the patient.

In bevorzugten Ausführungsformen des Verfahrens wird zusätzlich die Menge an entsprechendem Genprodukt in einer Kontrollprobe untersucht und mit der Menge an Genprodukt in der Patientenprobe verglichen. Bei der Kontrollprobe handelt es sich bevorzugt um die entsprechende Gewebeprobe eines Gesunden oder gesundes Pankreasgewebe eines an Pankreaskarzinom Erkrankten.In preferred embodiments of the method, in addition, the amount of corresponding gene product in a control sample is examined and compared with the amount of gene product in the patient sample. The control sample is preferably the corresponding tissue sample of a healthy or healthy pancreatic tissue of a pancreatic carcinoma sufferer.

In weiteren bevorzugten Ausführungsformen des Verfahrens wird zusätzlich die Menge an Genprodukt mindestens eines Referenzgens bestimmt.In further preferred embodiments of the method, the amount of gene product of at least one reference gene is additionally determined.

Bei dem Referenzgen handelt es sich vorzugsweise um ein Gen, das sowohl im Pankreaskarzinom als auch im normalen Pankreasepithelgewebe exprimiert wird und im Pankreaskarzinom keine veränderte Expressionshöhe gegenüber dem normalen Pankreasepithelgewebe erfährt. Bevorzugte Referenzgene sind konstitutiv exprimierte sog. household genes. Bevorzugte Referenzgene sind beta-Actin (NM_001101 ), Cyclophilin A (NM_021130) oder G6PD (NM_000402).The reference gene is preferably a gene which is expressed in pancreatic carcinoma as well as in normal pancreatic epithelial tissue and in pancreatic carcinoma does not undergo an altered expression level compared to normal pancreatic epithelial tissue. Preferred reference genes are constitutively expressed so-called household genes. Preferred reference genes are beta-actin (NM_001101), cyclophilin A (NM_021130) or G6PD (NM_000402).

In den Tabellen 3 und 4 sind weitere Gene aufgeführt, die im Pankreaskarzinom eine veränderte Expressionshöhe gegenüber dem normalen Pankreasepithelgewebe erfahren. Von diesen Genen war bereits vor der der Erfindung zugrundeliegenden Untersuchung des kompletten Transkriptoms bekannt, dass sie mit Pankreaskarzinom in Verbindung stehen.In Tables 3 and 4, further genes are listed which undergo an altered expression level in pancreatic carcinoma compared to the normal pancreatic epithelial tissue. Of these genes, it was already known prior to the invention of the entire transcriptome investigation that they are associated with pancreatic carcinoma.

In Tabelle 3 sind dabei Gene aufgeführt, deren Expression im duktalen Adenokarzinom des Pankreas im Mittel mindestens doppelt so hoch ist wie die Expression in normalem Pankreasepithelgewebe. D. h. der Quotient („Fold Change") aus den Expressionswerten im mikrodissektierten duktalen Adenomakarzinom des Pankreas (Mittel über 14 Patienten) und den Expressionswerten im gesunden mikrodissektierten duktalen Pankreasepithelgewebe (Mittel über 11 Personen) ist für diese Gene > 2. Wird für ein Gen der Quotient („Fold Change") 1000 angegeben, bedeutet dies, dass, dass das Gen nur in dem Tumor-, aber nicht in dem Normalgewebe nachgewiesen werden konnte. Die Gene sind ab Nummer 1268 fortlaufend nummeriert. Zur Charakterisierung werden jeweils die Identifikationsnummer des Affymetrix-Probensets, das Gensymbol, der Gen-Name, die chromosomale Lokalisation, die repräsentative Identifikationsnummer (ID), die Identifikationsnummer des Transkriptes und die des zugehörigen Proteins angegeben (Veröffentlichungsnummern in der Entrez- Datenbank des National Center for Biotechnology Information (NCBI), Bethesda, USA (http://www.ncbi.nih.gov/entrez/). Der q-Wert (in Prozent) gibt an, mit welchem statistischen Fehler das verwendete Auswerteprogramm eine differentielle Genexpression voraussagt. In Table 3, genes are listed whose expression in the ductal adenocarcinoma of the pancreas is on average at least twice as high as the expression in normal pancreatic epithelial tissue. Ie. the quotient (fold change) from the expression values in the microdissected ductal adenoma carcinoma of the pancreas (mean over 14 patients) and the expression values in the healthy microdissected ductal pancreas epithelium (mean over 11 persons) is> 2 for these genes ("Fold change") 1000, this means that the gene could be detected only in the tumor, but not in the normal tissue. The genes are consecutively numbered from number 1268. For characterization, the identification number of the Affymetrix sample set, the gene symbol, the gene name, the chromosomal localization, the representative identification number (ID), the identification number of the transcript and that of the corresponding protein are specified (publication numbers in the Entrez database of the National Center for Biotechnology Information (NCBI), Bethesda, USA (http://www.ncbi.nih.gov/entrez/) The q value (in percent) indicates the statistical error with which the evaluation program used predicts differential gene expression.

Tabelle 3:Table 3:

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1268 213150_at HOXA10 homeo box A10 BF792917 Hs.110637.0 NP 061824 Chr:7p15-p14 10001268 213150_at HOXA10 homeo box A10 BF792917 Hs.110637.0 NP 061824 Chr: 7p15-p14 1000

1269 210252_s_at MADD MAP-kinase activating AB002356 g2224656 NP_003673 Chr:11p11.2 1000 2.571322 death domain1269 210252_s_at MADD MAP kinase activating AB002356 g2224656 NP_003673 BC: 11p11.2 1000 2.571322 death domain

1270 202870_s_at CDC20 CDC20 cell division cycle NM_001255 g4557436 NPJ)01246 Chr.ip34.1 1000 4.830969 20 homolog (S. cerevisiae) /// CDC20 cell division cycle 20 homolog (S. cerevisiae)1270 202870_s_at CDC20 CDC20 cell division cycle NM_001255 g4557436 NPJ) 01246 Chr.ip34.1 1000 4.830969 20 homologue (S. cerevisiae) /// CDC20 cell division cycle 20 homologue (S. cerevisiae)

1271 208510_s_at PPARG peroxisome proliferative NM_015869 g7705548 NP_005028 Chr:3p25 14.21852 1.702877 activated receptor, gamma /// peroxisome proliferative activated receptor, gamma1271 208510_s_at PPARG peroxisome proliferative NM_015869 g7705548 NP_005028 Chr: 3p25 14.21852 1.702877 activated receptor, gamma /// peroxisome proliferative activated receptor, gamma

1272 202580 x at FOXM1 forkhead box M1 NM 021953 g11386144 NP 068772 Chr:12p13 9.09999 10.203011272 202580 x at FOXM1 forkhead box M1 NM 021953 g11386144 NP 068772 Chr: 12p13 9.09999 10.20301

1273 218542_at C10orf3 chromosome 10 open NM_018131 g8922501 NP_060601 Chr:10q23.33 9.02927 1.578435 reading frame 31273 218542_at C10orf3 chromosomes 10 open NM_018131 g8922501 NP_060601 Chr: 10q23.33 9.02927 1.578435 reading frame 3

1274 37892 at COL11A1 Collagen, type Xl, alpha 1 J04177 4876385 NP 001845 Chr:1p21 8.12914 12.533741274 37892 at COL11A1 Collagen, type XI, alpha 1 J04177 4876385 NP 001845 Chr: 1p21 8.12914 12.53374

1275 203819_s_at tMP-3 IGF-II mRNA-binding AU160004 Hs.79440.0 NP_006538 Chr:7p11 7.41914 3.612633 protein 31275 203819_s_at tMP-3 IGF-II mRNA binding AU160004 Hs.79440.0 NP_006538 Chr: 7p11 7.41914 3.612633 protein 3

1276 202095_s_at B1RC5 baculoviral IAP repeat- NM_001168 g4502144 NP_001159 Chr:17q25 5.49224 6.225758 containing 5 (survivin) /// baculoviral IAP repeat- containing 5 (survivin)1276 202095_s_at B1RC5 baculoviral IAP repeat- NM_001168 g4502144 NP_001159 Chr: 17q25 5.49224 6.225758 containing 5 (survivin) /// baculoviral IAP repeat- containing 5 (survivin)

1277 204170_s_at CKS2 CDC28 protein kinase NM_001827 g4502858 NPJ301818 Chr:9q22 5.44996 4.830969 regulatory subunit 2 /// CDC28 protein kinase regulatory subunit 21277 204170_s_at CKS2 CDC28 protein kinase NM_001827 g4502858 NPJ301818 Chr: 9q22 5.44996 4.830969 regulatory subunit 2 /// CDC28 protein kinase regulatory subunit 2

1278 209642 at BUB1 BUB1 budding uninhibited AF043294 g6970210 NP_004327 Chr:2q14 5.17194 3.612633 by benzimidazoles 1 homolog (yeast) /// BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast)1278 209642 at BUB1 BUB1 budding uninhibited AF043294 g6970210 NP_004327 Chr: 2q14 5.17194 3.612633 by benzimidazoles 1 homologue (yeast) /// BUB1 budding uninhibited by benzimidazoles 1 homologue (yeast)

1279 209792 s at KLK10 kallikrein 10 /// kallikrein 10 BC002710 gl 2803744 NP_002767 Chr:19q13.3- 4.91941 6.225758 q13.4 1279 209792 s at KLK10 kallikrein 10 /// kallikrein 10 BC002710 gl 2803744 NP_002767 Chr: 19q13.3- 4.91941 6.225758 q13.4

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1280 204286_s_at PMAIP1 phorbol-12-myristate-13- NM_021127 gl 0863922 NP_066950 Chr:18q21.32 4.9169 8.153992 acetate-induced protein 1 /// phorbol-12-myristate-13- acetate-induced protein 11280 204286_s_at PMAIP1 phorbol-12-myristate-13-NM_021127 gl 0863922 NP_066950 Chr: 18q21.32 4.9169 8.153992 acetate-induced protein 1 /// phorbol-12-myristate-13-acetate-induced protein 1

1281 211924_s_at PLAUR Plasminogen activator, AY029180 .gl 3641308 NP_002650 Chr:19q13 4.88546 11.06357 Urokinase receptor /// Plasminogen activator, Urokinase receptor1281 211924_s_a PLAUR Plasminogen activator, AY029180 .gl 3641308 NP_002650 Chr: 19q13 4.88546 11.06357 Urokinase receptor /// Plasminogen activator, urokinase receptor

1282 203108_at RAI3 retinoic acid induced 3 /// NM„003979 gl 2056470 NP_003970 Chr:12p13- 4.7401 3.612633 retinoic acid induced 3 P12.31282 203108_at RAI3 retinoic acid induced 3 /// NM " 003979 gl 2056470 NP_003970 Chr: 12p13- 4.7401 3.612633 retinoic acid induced 3 P12.3

1283 201467_s_at NQO1 NAD(P)H dehydrogenase, AI039874 Hs.80706.0 NP__000894 Chr:16q22.1 4.68911 2.571322 quinone 11283 201467_s_at NQO1 NAD (P) H dehydrogenase, AI039874 Hs.80706.0 NP__000894 Chr: 16q22.1 4.68911 2.571322 quinone 1

1284 204531_s_at BRCA1 breast cancer 1 , early onset NM_007295 g6552300 NP_009225 Chr:17q21 4.5618 0.353669 /// breast cancer 1 , eariy onset1284 204531_s_at BRCA1 breast cancer 1, early onset NM_007295 g6552300 NP_009225 Chr: 17q21 4.5618 0.353669 /// breast cancer 1, eariy onset

1285 203726 s at LAMA3 laminin, alpha 3 NM 000227 g4557710 NP 000218 Chr:18q11.2 4.54591 7.6464451285 203726 s at LAMA3 laminin, alpha 3 NM 000227 g4557710 NP 000218 Chr: 18q11.2 4.54591 7.646445

OO

1286 225387_at TM4SF9 transmembrane 4 AA059445 Hs.8037.0 NP_005714 Chr:4q23 4.50432 9.266777 superfamily member 91286 225387_at TM4SF9 transmembrane 4 AA059445 Hs.8037.0 NP_005714 Chr: 4q23 4.50432 9.266777 superfamily member 9

1287 214710 s at CCNB1 cyclin B1 BE407516 Hs.23960.2 NP 114172 Chr.5q12 4.45865 2.0095211287 214710 s at CCNB1 cyclin B1 BE407516 Hs.23960.2 NP 114172 Chr.5q12 4.45865 2.009521

1288 208079_s_at STK6 serine/threonine kinase 6 /// NM_003158 g4507278 NP_003149 Chr:20q13.2- 4.38175 4.149813 serine/threonine kinase 6 q13.31288 208079_s_at STK6 serine / threonine kinase 6 /// NM_003158 g4507278 NP_003149 Chr: 20q13.2- 4.38175 4.149813 serine / threonine kinase 6 q13.3

1289 201884 at CEACAM5 carcinoembryonic antigen- NM_004363 gl 1386170 NP_004354 Chr:19q13.1- 4.21221 3.306454 related cell adhesion q13.2 molecule 5 /// carcinoembryonic antigen- related cell adhesion molecule 51289 201884 at CEACAM5 carcinoembryonic antigen NM_004363 gl 1386170 NP_004354 Chr: 19q13.1- 4.21221 3.306454 related cell adhesion q13.2 molecule 5 /// carcinoembryonic antigen-related cell adhesion molecule 5

1290 206074_s_at HMGA1 high mobility group AT-hook NMJD02131 g4504432 NP_002122 Chr:6p21 4.11352 3.612633 1 /// high mobility group AT- hook 11290 206074_s_at HMGA1 high mobility group AT-hook NMJD02131 g4504432 NP_002122 BC: 6p21 4.11352 3.612633 1 /// high mobility group AT-hook 1

1291 225834_at MGC57827 Similar to RIKEN cDNA AL135396 Hs.18349.1 NP_997301 4.02836 12.80219 2700049P18 gene1291 225834_at MGC57827 Similar to RIKEN cDNA AL135396 Hs.18349.1 NP_997301 4.02836 12.80219 2700049P18 gene

1292 207165_at HMMR hyaluronan-mediated NM_012485 g7108350 NP_036616 Chr:5q33.2-qter 3.97806 4.149813 motility receptor (RHAMM)1292 207165_at HMMR hyaluronan-mediated NM_012485 g7108350 NP_036616 Chr: 5q33.2-qter 3.97806 4.149813 motility receptor (RHAMM)

1293 217109_at MUC4 mucin 4, tracheobronchial AJ242547 Hs.198267.2 NP 004523 Chr:3q29 3.89312 3.62324 1293 217109_at MUC4 mucin 4, tracheobronchial AJ242547 Hs.198267.2 NP 004523 Chr: 3q29 3.89312 3.62324

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung ChangeNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change

1294 222608_s_at ANLN anillin, actin binding protein AK023208 Hs.62180.0 NP_061155 Chr:7p15-p14 3.87492 13.64212 (scraps homolog, Drosophila)1294 222608_s_at ANLN anillin, actin binding protein AK023208 Hs.62180.0 NP_061155 Chr: 7p15-p14 3.87492 13.64212 (scraps homologous, Drosophila)

1295 201292_at TOP2A topoisomerase (DNA) Il AL561834 Hs.156346.0 NP_001058 Chr:17q21-q22 3.67605 4.149813 alpha 17OkDa1295 201292_at TOP2A topoisomerase (DNA) II AL561834 Hs.156346.0 NP_001058 Chr: 17q21-q22 3.67605 4.149813 alpha 17OkDa

1296 225295_at SLC39A10 solute carrier family 39 (zinc AB033091 Hs.24936.0 — Chr:2q33.1 3.56639 13.5285 transporter), member 101296 225295_at SLC39A10 solute carrier family 39 (zinc AB033091 Hs.24936.0 - Chr: 2q33.1 3.56639 13.5285 transporter), member 10

1297 203213_at CDC2 cell division cycle 2, G1 to S AL524035 Hs.184572.0 NP_001777 Chr:10q21.1 3.52304 1.578435 and G2 to M1297 203213_at CDC2 cell division cycle 2, G1 to S AL524035 Hs.184572.0 NP_001777 B: 10q21.1 3.52304 1.578435 and G2 to M

1298 212942 s at KIAA1199 KIAA1199 protein AB033025 Hs.50081.1 NP 061159 Chr:15q 3.41813 10.203011298 212942 s at KIAA1199 KIAA1199 protein AB033025 Hs.50081.1 NP 061159 Chr: 15q 3.41813 10.20301

1299 203939_at NT5E 5'-nucleotidase, ecto NM_002526 g4505466 NP_002517 Chr:6q14-q21 3.38103 1.578435 (CD73) /// 5'-nucleotidase, ecto (CD73)1299 203939_at NT5E 5'-nucleotidase, ecto NM_002526 g4505466 NP_002517 Chr: 6q14-q21 3.38103 1.578435 (CD73) /// 5'-nucleotidase, ecto (CD73)

1300 205479_s_at PLAU Plasminogen activator, NM_002658 g4505862 NP_002649 Chr:10q24 3.32176 13.40329 Urokinase /// Plasminogen activator, Urokinase1300 205479_s_at PLAU Plasminogen activator, NM_002658 g4505862 NP_002649 Chr: 10q24 3.32176 13.40329 Urokinase /// Plasminogen activator, urokinase

1301 220658 s at ARNTL2 aryl hydrocarbon receptor NM_020183 g9910367 NP_064568 Chr:12p12.2- 3.3148 1.578435 ^ nuclear translocator-like 2 p11.2 o1301 220658 s at ARNTL2 aryl hydrocarbon receptor NM_020183 g9910367 NP_064568 Chr: 12p12.2- 3.3148 1.578435 ^ nuclear translocator-like 2 p11.2 o

Ul /// aryl hydrocarbon receptor nuclear translocator-like 2Ul /// aryl hydrocarbon receptor nuclear translocator-like 2

1302 202712_ _s_at CKMT1 creatine kinase, NM_020990 gl 1641403 NP_ _066270 Chr:15q15 3.31168 13.40329 mitochondrial 1 (ubiquitous) /// creatine kinase, mitochondrial 1 (ubiquitous)1302 202712_ _s_at CKMT1 creatine kinase, NM_020990 gl 1641403 NP_ _066270 Chr: 15q15 3.31168 13.40329 mitochondrial 1 (ubiquitous) /// creatine kinase, mitochondrial 1 (ubiquitous)

1303 210809_ _s_at POSTN periostin, Osteoblast D13665 g393318 NP. _006466 Chr:13q13.3 3.30048 4.149813 specific factor /// periostin, Osteoblast specific factor1303 210809_ _s_at POSTN periostin, osteoblast D13665 g393318 NP. _006466 Chr: 13q13.3 3.30048 4.149813 specific factor /// periostin, osteoblast specific factor

1304 203878_ _s_at MMP11 matrix metalloproteinase 11 NM_005940 gl 3027795 NP. _005931 Chr:22q11.23 3.27537 2.009521 (stromelysin 3) /// matrix metalloproteinase 11 (stromelysin 3)1304 203878_ _s_at MMP11 matrix metalloproteinase 11 NM_005940 gl 3027795 NP . _005931 Chr: 22q11.23 3.27537 2.009521 (stromelysin 3) /// matrix metalloproteinase 11 (stromelysin 3)

1305 212022_ _s_at MKI67 antigen identified by BF001806 Hs.80976.1 NP. _002408 Chr:10q25-qter 3.18835 13.40329 monoclonal antibody Ki-67 1305 212022_ _s_at MKI67 antigen identified by BF001806 Hs.80976.1 NP . _002408 Chr: 10q25-qter 3.18835 13.40329 monoclonal antibody Ki-67

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

1306 201195 s at SLC7A5 solute carrier family 7 AB018009 g5926731 NP_003477 Chr:16q24.3 3.13152 3.61384 (cationic amino acid transporter, y+ system), member 5 /// solute carrier family 7 (cationic amino acid transporter, y+ system), member 5Solids carrier family 7 AB018009 g5926731 NP_003477 Chr: 16q24.3 3.13152 3.61384 (cationic amino acid transporter, y + system), member 5 /// solute carrier family 7 (cationic amino acid transporter, y + system), member 5

1307 205483_s_at G1 P2 interferon, alpha-inducible NM_005101 g4826773 NP_005092 Chr:1p36.33 3.12086 12.53374 protein (clone IFI-15K) /// interferon, alpha-inducible protein (clone IFI-15K)1307 205483_s_at G1 P2 interferon, alpha-inducible NM_005101 g4826773 NP_005092 Chr: 1p36.33 3.12086 12.53374 protein (clone IFI-15K) /// interferon, alpha-inducible protein (clone IFI-15K)

1308 204351_at S100P S100 calcium binding NM_005980 g5174662 NP_005971 Chr:4p16 3.11871 4.149813 protein P /// S 100 calcium binding protein P1308 204351_at S100P S100 calcium binding NM_005980 g5174662 NP_005971 Chr: 4p16 3.11871 4.149813 protein P /// S 100 calcium binding protein P

1309 201850_at CAPG capping protein (actin NM_001747 g4502560 NP_001738 Chr:2cen-q24 3.05331 1.578435 filament), gelsolin-like /// capping protein (actin filament), gelsolin-like O1309 201850_at CAPG capping protein (actin NM_001747 g4502560 NP_001738 Chr: 2cen-q24 3.05331 1.578435 filament), gelsolin-like // capping protein (actin filament), gelsolin-like O

1310 204602_at DKK1 dickkopf homolog 1 NMJ)12242 g7110718 NP_036374 Chr: 10q 11.2 3.046 7.646445 (Xenopus laevis) /// dickkopf homolog 1 (Xenopus laevis)1310 204602_at DKK1 thick-head homolog 1 NMJ) 12242 g7110718 NP_036374 Chr: 10q 11.2 3.046 7.646445 (Xenopus laevis) /// thick-head homolog 1 (Xenopus laevis)

1311 205499_at SRPUL sushi-repeat protein /// NMJ)14467 g7657618 NP_055282 Chr.xq21.33- 3.04048 6.991358 sushi-repeat protein q231311 205499_at SRPUL sushi-repeat protein /// NMJ) 14467 g7657618 NP_055282 Chr.xq21.33- 3.04048 6.991358 sushi-repeat protein q23

1312 204855 at SERPINB5 serine (or cysteine) NM_002639 g4505788 NP_002630 Chr:18q21.3 3.02744 10.20301 Proteinase iπhibitor, clade B (ovalbumin), member 5 /// serine (or cysteine) Proteinase inhibitor, clade B (ovalbumin), member 51312 204855 at SERPINB5 serine (or cysteine) NM_002639 g4505788 NP_002630 Chr: 18q21.3 3.02744 10.20301 Proteinase inhibitor, clade B (ovalbumin), member 5 /// serine (or cysteine) Proteinase inhibitor, clade B (ovalbumin), member 5

1313 205081, CRIP1 cysteine-rich protein 1 NMJ)OI 311 g4503046 NP. _001302 Chr:7q11.23 3.02177 2.571322 (intestinal) /// cysteine-rich protein 1 (intestinal)1313 205081 , CRIP1 cysteine-rich protein 1 NMJ) OI 311 g4503046 NP. _001302 Chr: 7q11.23 3.02177 2.571322 (intestinal) /// cysteine-rich protein 1 (intestinal)

1314 214830, _at SLC38A6 solute carrier family 38, AI537540 Hs.71168.0 NP. _722518 Chr:14q23.1 2.99801 5.685182 member 6 1314 214830 , _at SLC38A6 solute carrier family 38, AI537540 Hs.71168.0 NP. _722518 Chr: 14q23.1 2.99801 5.685182 member 6

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1315 203554_x_at PTTG1 pituitary tumor-transforming NM_004219 gl 1038651 NP_004210 Chr:5q35.1 2.98209 3.61384 1 /// pituitary tumor- transforming 11315 203554_x_at PTTG1 pituitary tumor-transforming NM_004219 gl 1038651 NP_004210 Chr: 5q35.1 2.98209 3.61384 1 /// pituitary tumor-transforming 1

1316 205927 s at CTSE cathepsin E /// cathepsin E NM 001910 g4503144 NP 001901 Chr:1q31 2.89469 3.613841316 205927 s at CTSE cathepsin E /// cathepsin E NM 001910 g4503144 NP 001901 Chr: 1q31 2.89469 3.61384

1317 205534_at PCDH7 BH-protocadherin (brain- NM_002589 g4505634 NPJ302580 Chr:4p15 2.86035 3.612633 heart)1317 205534_at PCDH7 BH-protocadherin (brain- NM_002589 g4505634 NPJ302580 Chr: 4p15 2.86035 3.612633 heart)

1318 202411_at IFI27 interferon, alpha-inducible NM__005532 g5031780 NP_005523 Chr:14q32 2.84206 11.06357 protein 27 /// interferon, alpha-inducible protein 271318 202411_at IFI27 interferon, alpha-inducible NM__005532 g5031780 NP_005523 Chr: 14q32 2.84206 11.06357 protein 27 /// interferon, alpha-inducible protein 27

1319 208711_s_at CCND1 cyclin D1 (PRAD1 : BC000076 gl 2652656 NP_001749 Chr:11q13 2.82367 8.779357 parathyroid adenomatosis D1319 208711_s_at CCND1 cyclin D1 (PRAD1: BC000076 gl 2652656 NP_001749 Chr: 11q13 2.82367 8.779357 parathyroid adenomatosis D

1320 200737 at PGK1 phosphoglycerate kinase 1 NM 000291 g4505762 NP 000282 Chr:xq13 2.79459 9.6257151320 200737 at PGK1 phosphoglycerate kinase 1 NM 000291 g4505762 NP 000282 Chr: xq13 2.79459 9.625715

1321 204767_s_at FEN1 flap structure-specific BC000323 gl 2653112 NP_004102 Chr:11q12 2.77031 1.578435 endonuclease 1 /// flap structure-specific endonuclease 11321 204767_s_at FEN1 flap structure-specific BC000323 gl 2653112 NP_004102 Chr: 11q12 2.77031 1.578435 endonuclease 1 /// flap structure-specific endonuclease 1

1322 212464 s at FN1 fibronectin 1 X02761 Hs.287820.1 NP 002017 Chr:2q34 2.71039 1.578435 O -41322 212464 s at FN1 fibronectin 1 X02761 Hs.287820.1 NP 002017 B: 2q34 2.71039 1.578435 O -4

1323 213453_x_at GAPD glyceraldehyde-3- BF689355 Hs.169476.4 NP_002037 Chr:12p13 2.69085 3.612633 phosphate dehydrogenase1323 213453_x_at GAPD glyceraldehyde-3-BF689355 Hs.169476.4 NP_002037 Chr: 12p13 2.69085 3.612633 phosphate dehydrogenase

1324 209016 s at KRT7 keratin 7 /// keratin 7 BC002700 gl 2803726 NP 005547 Chr:12q12-q13 2.68087 11.063571324 209016 s at KRT7 keratin 7 /// keratin 7 BC002700 gl 2803726 NP 005547 Chr: 12q12-q13 2.68087 11.06357

1325 227062_at TncRNA trophoblast-derived AU155361 Hs.79706.1 — Chr:11q13.1 2.67851 13.5285 noncoding RNA1325 227062_at TncRNA trophoblast-derived AU155361 Hs.79706.1 - Chr: 11q13.1 2.67851 13.5285 noncoding RNA

1326 225265 at Hs.239870.1 2.53063 11.552521326 225265 at Hs.239870.1 2.53063 11.55252

1327 33323 r at SFN stratifin X57348 4866611 NP 006133 Chr:1p35.3 2.52179 1.5784351327 33323 r at SFN stratifin X57348 4866611 NP 006133 Chr: 1p35.3 2.52179 1.578435

1328 201250 s at SLC2A1 solute carrier family 2 NM_006516 g5730050 NP_006507 Chr:1p35-p31.3 2.47262 10.20301 (facilitated glucose transporter), member 1 /// solute carrier family 2 (facilitated glucose transporter), member 1Solubilizer family 2 NM_006516 g5730050 NP_006507 Chr: 1p35-p31.3 2.47262 10.20301 (facilitated glucose transporter), member 1 /// solute carrier family 2 (facilitated glucose transporter), member 1

1329 203362 s at MAD2L1 MAD2 mitotic arrest NM_002358 g6466452 NP_002349 Chr:4q27 2.46834 0.095261 deficient-like 1 (yeast) /// MAD2 mitotic arrest deficient-like 1 (yeast) 1329 203362 s at MAD2L1 MAD2 mitotic arrest NM_002358 g6466452 NP_002349 Chr: 4q27 2.46834 0.095261 deficient-like 1 (yeast) /// MAD2 mitotic arrest deficient-like 1 (yeast)

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valυe Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-valυe Sample Number ID Protein ID Localization Change (%)

1330 214974_x_at CXCL5 chemokine (C-X-C motif) AK026546 Hs.287716.0 NP_002985 Chr:4q12-q13 2.4528 9.625715 ligand 51330 214974_x_at CXCL5 chemokines (C-X-C motif) AK026546 Hs.287716.0 NP_002985 Chr: 4q12-q13 2.4528 9.625715 ligand 5

1331 201231_s_at ENO1 enolase 1 , (alpha) /// NM_001428 g4503570 NPJ301419 Chr:1p36.3- 2.44226 1.578435 enolase l, (alpha) p36.21331 201231_s_at ENO1 enolase 1, (alpha) /// NM_001428 g4503570 NPJ301419 Chr: 1p36.3- 2.44226 1.578435 enolase I, (alpha) p36.2

1332 201105_at LGALS1 lectin, galactoside-binding, NM_002305 g6006015 NPJ)02296 Chr:22q13.1 2.44057 6.225758 solubie, 1 (galectin 1) /// lectin, galactoside-binding, solubie, 1 (galectin 1)1332 201105_at LGALS1 lectin, galactoside-binding, NM_002305 g6006015 NPJ) 02296 Chr: 22q13.1 2.44057 6.225758 solubie, 1 (galectin 1) /// lectin, galactoside-binding, solubie, 1 (galectin 1)

1333 202338_at TK1 thymidine kinase 1 , solubie NM_003258 g4507518 NP_003249 Chr:17q23.2- 2.43793 4.436744 /// thymidine kinase 1 , q25.3 solubie1333 202338_at TK1 thymidine kinase 1, solubia NM_003258 g4507518 NP_003249 Chr: 17q23.2- 2.43793 4.436744 /// thymidine kinase 1, q25.3 solubie

1334 201456_s_at BUB3 BUB3 budding uninhibited AU160695 Hs.40323.0 NP_004716 Chr:10q26 2.43565 5.685182 by benzimidazoles 3 homolog (yeast)1334 201456_s_at BUB3 BUB3 budding uninhibited AU160695 Hs.40323.0 NP_004716 Chr: 10q26 2.43565 5.685182 by benzimidazoles 3 homolog (yeast)

1335 217733_s_at TMSB10 thymosin, beta 10 /// NMJD21103 gl 0863894 NP_066926 Chr:2p11.2 2.43233 1.578435 thymosin, beta 101335 217733_s_at TMSB10 thymosin, beta 10 /// NMJD21103 gl 0863894 NP_066926 Chr: 2p11.2 2.43233 1.578435 thymosin, beta 10

1336 201663_s_at SMC4L1 SMC4 structural NM_005496 g4885112 NP__005487 Chr:3q26.1 2.40184 6.991358 maintenance of chromosomes 4-like 1 (yeast)1336 201663_s_at SMC4L1 SMC4 structural NM_005496 g4885112 NP__005487 Chr: 3q26.1 2.40184 6.991358 maintenance of chromosomes 4-like 1 (yeast)

1337 219026_s_at RASAL2 RAS protein activator like 2 NMJD04841 g4758807 NP_004832 Chr:1q24 2.39773 7.646445 § /// RAS protein activator like 21337 219026_s_at RASAL2 RAS protein activator like 2 NMJD04841 g4758807 NP_004832 Chr: 1q24 2.39773 7.646445 § /// RAS protein activator like 2

1338 206994 at CST1 cystatin SN /// cystatin SN NM 001899 g4503108 NP 001889 Chr:20p11.21 2.39487 5.6851821338 206994 at CST1 cystatin SN /// cystatin SN NM 001899 g4503108 NP 001889 Chr: 20p11.21 2.39487 5.685182

1339 200820 at PSMD8 proteasome (prosome, NM_002812 g4506232 NP_002803 Chr:19q13.13 2.38736 3.612633 macropain) 26S subunit, πon-ATPase, 8 /// proteasome (prosome, macropain) 26S subunit, non-ATPase, 81339 200820 at PSMD8 proteasome (prosome, NM_002812 g4506232 NP_002803 Chr: 19q13.13 2.38736 3.612633 macropain) 26S subunit, πon ATPase, 8 /// proteasome (prosome, macropain) 26S subunit, non-ATPase, 8

1340 211864_ _s_at FER1L3 fer-1-like 3, myoferlin (C. AF207990 gl 0834586 NP. _038479 Chr:10q24 2.3862 6.225758 elegans)1340 211864_ _s_at FER1L3 fer-1-like 3, myoferlin (C. AF207990 gl 0834586 NP. _038479 Chr: 10q24 2.3862 6.225758 elegans)

1341 212242. _at TUBA1 tubulin, alpha 1 (testis AL565074 Hs.75318.0 NP. .005991 Chr:2q36.1 2.37786 5.685182 specific) 1341 212242. _at TUBA1 tubulin, alpha 1 (testis AL565074 Hs.75318.0 NP .005991 Chr: 2q36.1 2.37786 5.685182 specific)

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1342 201926_s_at DAF decay accelerating factor BC001288 gl 2654888 NP_000565 Chr:1q32 2.34643 4.436744 for complement (CD55, Cromer blood group System)1342 201926_s_a DAF decay accelerating factor BC001288 gl 2654888 NP_000565 Chr: 1q32 2.34643 4.436744 for complement (CD55, Cromer blood group System)

1343 206284_x_at CLTB clathrin, light Polypeptide NM_001834 g4502900 NP_001825 Chr:5q35 2.3376 5.685182 (Leb)1343 206284_x_at CLTB clathrin, light polypeptides NM_001834 g4502900 NP_001825 Chr: 5q35 2.3376 5.685182 (Leb)

1344 210715_s_at SPINT2 serine protease inhibitor, AF027205 g2598967 NP_066925 Chr:19q13.1 2.31815 4.830969 Kunitz type, 2 /// serine Protease inhibitor, Kunitz type, 21344 210715_s_at SPINT2 serine protease inhibitor, AF027205 g2598967 NP_066925 Chr: 19q13.1 2.31815 4.830969 Kunitz type, 2 /// serine Protease inhibitor, Kunitz type, 2

1345 209233_at C2F C2f protein U72514 g2276395 NP 006322 Chr:12p13 2.31448 10.203011345 209233_at C2F C2f protein U72514 g2276395 NP 006322 Chr: 12p13 2.31448 10.20301

1346 201853_s_at CDC25B cell division eyele 25B /// NM_021873 g11641412 NP_004349 Chr:20p13 2.28393 6.225758 cell division eyele 25B1346 201853_s_at CDC25B cell division eyele 25B /// NM_021873 g11641412 NP_004349 Chr: 20p13 2.28393 6.225758 cell division eyele 25B

1347 218322_s_at ACSL5 acyl-CoA synthetase long- NM_016234 gl 2669912 NP_057318 Chr:10q25.1- 2.26919 3.612633 chain family member 5 /// q25.2 acyl-CoA synthetase long- chain family member 51347 218322_s_at ACSL5 acyl-CoA synthetase long NM_016234 gl 2669912 NP_057318 Chr: 10q25.1- 2.26919 3.612633 chain family member 5 /// q25.2 acyl-CoA synthetase long-chain family member 5

OO

1348 210538_s_at BIRC3 baculoviral IAP repeat- U37546 gl 145290 NP_001156 Chr:11q22 2.26863 8.779357 containing 3 /// baculoviral IAP repeat-containing 31348 210538_s_at BIRC3 baculoviral IAP repeat- U37546 gl 145290 NP_001156 Chr: 11q22 2.26863 8.779357 containing 3 /// baculoviral IAP repeat-containing 3

1349 200660_at S100A11 S100 calcium binding NM_005620 g5032056 NP_005611 Chr:1q21 2.21304 1.578435 protein A11 (calgizzarin) /// S100 calcium binding protein A11 (calgizzarin)1349 200660_at S100A11 S100 calcium binding NM_005620 g5032056 NP_005611 Chr: 1q21 2.21304 1.578435 protein A11 (calgizzarin) /// S100 calcium binding protein A11 (calgizzarin)

1350 212353 at SULF1 sulfatase 1 AI479175 Hs.70823.0 NP 055985 Chr:8q13.2 2.20207 11.063571350 212353 at SULF1 sulfatase 1 AI479175 Hs.70823.0 NP 055985 Chr: 8q13.2 2.20207 11.06357

1351 222555_s_at MRPL44 mitochondrial ribosomal AI338045 Hs.203559.0 NP_075066 Chr:2q36.3 2.20156 11.55252 protein L441351 222555_s_at MRPL44 mitochondrial ribosomal AI338045 Hs.203559.0 NP_075066 Chr: 2q36.3 2.20156 11.55252 protein L44

1352 217691_x_at SLC16A3 solute carrier family 16 AA853175 Hs.333049.0 NP_004198 Chr:17q25 2.17864 11.70204 (monocarboxylic acid transporters), member 31352 217691_x_at SLC16A3 solute carrier family 16 AA853175 Hs.333049.0 NP_004198 Chr: 17q25 2.17864 11.70204 (monocarboxylic acid transporters), member 3

1353 202806 at DBN1 drebrin 1 /// drebrin 1 NM 004395 g4758123 NP 004386 Chr:5q35.3 2.17682 13.40329 1353 202806 at DBN1 drebrin 1 /// drebrin 1 NM 004395 g4758123 NP 004386 Chr: 5q35.3 2.17682 13.40329

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal Region q-value

Probennummer ID Protein ID Lokalisierung Change (%)Sample ID ID Protein ID Localization Change (%)

1354 212296 at PSMD14 proteasome (prosome, NM_005805 Hs.178761.0 NP_005796 Chr:2q24.3 2.17639 1.578435 macropain) 26S subunit, non-ATPase, 14 /// proteasome (prosome, macropain) 26S subunit, non-ATPase, 141354 212296 at PSMD14 proteasome (prosome, NM_005805 Hs.178761.0 NP_005796 Chr: 2q24.3 2.17639 1.578435 macropain) 26S subunit, non-ATPase, 14 /// proteasome (prosome, macropain) 26S subunit, non-ATPase, 14

1355 204620 s at CSPG2 chondroitin sulfate NM_004385 g4758081 NP_004376 Chr:5q14.3 2.16998 10.20301 proteoglycan 2 (versican) /// chondroitin sulfate proteoglycan 2 (versican)1355 204620 s at CSPG2 chondroitin sulfate NM_004385 g4758081 NP_004376 Chr: 5q14.3 2.16998 10.20301 proteoglycan 2 (versican) /// chondroitin sulfate proteoglycan 2 (versican)

1356 206571 s at MAP4K4 mitogen-activated protein NM_004834 g4758523 NP_004825 Chr:2q11.2-q12 2.15075 7.646445 kinase kinase kinase kinase 4 /// mitogen-activated protein kinase kinase kinase kinase 41356 206571 s at MAP4K4 mitogen-activated protein NM_004834 g4758523 NP_004825 Chr: 2q11.2-q12 2.15075 7.646445 kinase kinase kinase kinase 4 /// mitogen-activated protein kinase kinase kinase kinase 4

1357 211297 s at CDK7 cyclin-dependent kinase 7 L20320 g348242 NP_001790 Chr:5q12.1 2.15059 11.70204 (MO15 homolog, Xenopus laevis, cdk-activating kinase) /// cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk- activating kinase)1357 211297 s at CDK7 cyclin-dependent kinase 7 L20320 g348242 NP_001790 Chr: 5q12.1 2.15059 11.70204 (MO15 homolog, Xenopus laevis, cdk-activating kinase) /// cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk activating kinase)

1358 200966. _x_at ALDOA aldolase A, fructose- NM_000034 g4557304 NP. .000025 Chr:16q22-q24 2.14844 1.578435 bisphosphate /// aldolase A, fructose-bisphosphate1358 200966. _x_at ALDOA aldolase A, fructose NM_000034 g4557304 NP . .000025 Chr: 16q22-q24 2.14844 1.578435 bisphosphates /// aldolase A, fructose bisphosphates

1359 201189. _s_at ITPR3 inositol 1,4,5-triphosphate NM_002224 g4504794 NP. JD02215 Chr:6p21 2.1244 13.40329 receptor, type 3 /// inositol 1 ,4,5-triphosphate receptor, type 31359 201189. _s_at ITPR3 inositol 1,4,5-triphosphate NM_002224 g4504794 NP . JD02215 Chr: 6p21 2.1244 13.40329 receptor, type 3 /// inositol 1, 4,5-triphosphate receptor, type 3

1360 201251. _at PKM2 pyruvate kinase, muscle /// NM_002654 g4505838 NP. .002645 Chr:15q22 2.10284 6.225758 pyruvate kinase, muscle1360 201251. _at PKM2 pyruvate kinase, muscle /// NM_002654 g4505838 NP. .002645 Chr: 15q22 2.10284 6.225758 pyruvate kinase, muscle

1361 212312. _at COX4I2 cytochrome c oxidase AL117381 Hs.305890.1 NP. .115998 Chr.20q11.21 2.09715 3.61384 subunit IV isoform 2 1361 212312. _at COX4I2 cytochrome c oxidase AL117381 Hs.305890.1 NP. .115998 Chr.20q11.21 2.09715 3.61384 subunit IV isoform 2

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1362 201037_at PFKP phosphofructokinase, NM_002627 gl 1321600 NPJD02618 Chr:10p15.3- 2.09187 7.646445 platelet /// p15.2 phosphofructokinase, platelet1362 201037_at PFKP phosphofructokinase, NM_002627 gl 1321600 NPJD02618 Chr: 10p15.3- 2.09187 7.646445 platelet /// p15.2 phosphofructokinase, platelet

1363 213741_s_at KPNA1 karyopherin alpha 1 BF575685 Hs.169149.1 NP_002255 Chr:3q21 2.07961 4.436744 (importin alpha 5)1363 213741_s_at KPNA1 karyopherin alpha 1 BF575685 Hs.169149.1 NP_002255 Chr: 3q21 2.07961 4.436744 (importin alpha 5)

1364 201650 at KRT19 keratin 19 /// keratin 19 NM 002276 g4504916 NP 002267 Chr:17q21.2 2.07469 8.1539921364 201650 at KRT19 keratin 19 /// keratin 19 NM 002276 g4504916 NP 002267 Chr: 17q21.2 2.07469 8.153992

1365 200923_at LGALS3BP lectin, galactoside-binding, NM_005567 g6006016 NP_005558 Chr:17q25 2.0567 2.009521 soluble, 3 binding protein /// lectin, galactoside-binding, soluble, 3 binding protein1365 200923_at LGALS3BP lectin, galactoside-binding, NM_005567 g6006016 NP_005558 Chr: 17q25 2.0567 2.009521 soluble, 3 binding protein /// lectin, galactoside-binding, soluble, 3 binding protein

1366 201202_at PCNA proliferating cell nuclear NM_002592 g4505640 NP_002583 Chr:20pter-p12 2.04802 2.571322 antigen /// proliferating cell nuclear antigen1366 201202_at PCNA proliferating cell nuclear NM_002592 g4505640 NP_002583 Chr: 20pter-p12 2.04802 2.571322 antigen /// proliferating cell nuclear antigen

1367 202655_at ARMET arginine-rich, mutated in NM_006010 g5174392 NP_006001 Chr:3p21.1 2.04687 3.61384 early stage tumors /// arginine-rich, mutated in early stage tumors1367 202655_at ARMET arginine-rich, mutated in NM_006010 g5174392 NP_006001 Chr: 3p21.1 2.04687 3.61384 early stage tumors /// arginine-rich, mutated in early stage tumors

1368 204489_s_at CD44 CD44 antigen (homing NM_000610 gl 0835162 NP_000601 Chr:11p13 2.0327 11.70204 function and Indian blood group System)1368 204489_s_at CD44 CD44 antigen (homing NM_000610 gl 0835162 NP_000601 Chr: 11p13 2.0327 11.70204 function and Indian blood group system)

1369 209969_s_at STAT1 Signal transducer and BC002704 gl 2803734 NP_009330 Chr:2q32.2 2.0275 7.646445 activator of transcription 1 , 91 kDa1369 209969_s_at STAT1 Signal transducer and BC002704 gl 2803734 NP_009330 Chr: 2q32.2 2.0275 7.646445 activator of transcription 1, 91 kDa

1370 201897_s_at CKS 1 B CDC28 protein kinase NM_001826 g4502856 NP_001817 Chr:1q21.2 2.02532 3.612633 regulatory subunit 1 B /// CDC28 protein kinase regulatory subunit 1 B1370 201897_s_at CKS 1 B CDC28 protein kinase NM_001826 g4502856 NP_001817 Chr: 1q21.2 2.02532 3.612633 regulatory subunit 1 B /// CDC28 protein kinase regulatory subunit 1 B

1371 203083_at THBS2 thrombospondin 2 /// NM_003247 g4507486 NP_003238 Chr:6q27 2.0243 7.646445 thrombospondin 21371 203083_at THBS2 thrombospondin 2 /// NM_003247 g4507486 NP_003238 Chr: 6q27 2.0243 7.646445 thrombospondin 2

1372 218677_at S100A14 S100 calcium binding NM_020672 gl 0190711 NP_065723 Chr:1q21.1 2.02382 14.22309 protein A14 /// S100 calcium binding protein A141372 218677_at S100A14 S100 calcium binding NM_020672 gl 0190711 NP_065723 Chr: 1q21.1 2.02382 14.22309 protein A14 /// S100 calcium binding protein A14

1373 212481_s_at TPM4 tropomyosin 4 AI214061 Hs.250641.1 NP 003281 Chr:19p13.1 2.02019 9.625715 1373 212481_s_at TPM4 tropomyosin 4 AI214061 Hs.250641.1 NP 003281 Chr: 19p13.1 2.02019 9.625715

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript ID Repräsentative Chromosomale FoId q-value Probennummer ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript ID Representative Chromosomal FoId q-value Sample Number ID Protein ID Localization Change (%)

1374 218084_x_at FXYD5 FXYD domain containing NM_014164 gl 1612664 NP__054883 Chr:19q12- 2.01401 6.991358 ion transport regulator 5 /// q13.1 FXYD domain containing ion transport regulator 51374 218084_x_at FXYD5 FXYD containing NM_014164 gl 1612664 NP__054883 Chr: 19q12- 2.01401 6.991358 ion transport regulator 5 /// q13.1 FXYD domain containing ion transport regulator 5

1375 202488_s_at FXYD3 FXYD domain containing NM_005971 gl 1612675 NP_005962 Chr:19q13.13 2.0118 4.830969 ion transport regulator 3 /// FXYD domain containing ion transport regulator 3FXYD3 FXYD containing NM_005971 gl 1612675 NP_005962 Chr: 19q13.13 2.0118 4.830969 ion transport regulator 3 /// FXYD domain containing ion transport regulator 3

1376 20971 O_at GATA2 GATA binding protein 2 AL563460 Hs.760.1 NP 002041 Chr:3q21.3 0.48758 4.4367441376 20971 O_at GATA2 GATA binding protein 2 AL563460 Hs.760.1 NP 002041 Chr: 3q21.3 0.48758 4.436744

1377 203131 at PDGFRA platelet-derived growth NM 006206 g5453869 NP 006197 Chr:4q11-q13 0.40793 0.201325 factor receptor, alpha Polypeptide /// platelet- derived growth factor receptor, alpha Polypeptide 1377 203131 at PDGFRA platelet-derived growth NM 006206 g5453869 NP 006197 Chr: 4q11-q13 0.40793 0.201325 factor receptor, alpha polypeptides /// platelet-derived growth factor receptor, alpha polypeptides

In Tabelle 4 sind Gene aufgeführt, deren Expression im duktalen Adenokarzinom des Pankreas im Mittel maximal halb so hoch ist wie die Expression in normalem Pankreasepithelgewebe. D. h. der Quotient („Fold Change") aus den Expressionswerten im mikrodissektierten duktalen Adenomakarzinom des Pankreas (Mittel über 14 Patienten) und den Expressionswerten im gesunden mikrodissektierten duktalen Pankreasepithelgewebe (Mittel über 11 Personen) ist für diese Gene < 0,5. Wird für ein Gen der Quotient („Fold Change") 1000 angegeben, bedeutet dies, dass das Gen nur im Normalgewebe, aber nicht im Tumorgewebe nachgewiesen werden konnte. Die Gene sind ab der Nummer 1378 fortlaufend nummeriert. Zur Charakterisierung werden jeweils die Identifikationsnummer des Affymetrix-Probensets, das Gensymbol, der Gen-Name, die chromosomale Lokalisation, die repräsentative Identifikationsnummer (ID), die Identifikationsnummer des Transkriptes und die des zugehörigen Proteins angegeben (Veröffentlichungsnummern in der Entrez- Datenbank des National Center for Biotechnology Information (NCBI), Bethesda, USA (http://www.ncbi.nih.gov/entrez/). Der q-Wert (in Prozent) gibt an, mit welchem statistischen Fehler das verwendete Auswerteprogramm eine differentielle Genexpression voraussagt. Table 4 lists genes whose expression in the ductal adenocarcinoma of the pancreas is on average at most half as high as the expression in normal pancreatic epithelial tissue. Ie. the fold change from the expression levels in the microdissected ductal adenoma carcinoma of the pancreas (mean over 14 patients) and the expression values in the healthy microdissected ductal pancreas epithelium (mean over 11 persons) is <0.5 for these genes the quotient ("fold change") indicated 1000, this means that the gene could be detected only in normal tissue, but not in tumor tissue. The genes are sequentially numbered from the number 1378. For characterization, the identification number of the Affymetrix sample set, the gene symbol, the gene name, the chromosomal localization, the representative identification number (ID), the identification number of the transcript and that of the corresponding protein are specified (publication numbers in the Entrez database of the National Center for Biotechnology Information (NCBI), Bethesda, USA (http://www.ncbi.nih.gov/entrez/) The q value (in percent) indicates the statistical error with which the evaluation program used predicts differential gene expression.

Tabelle 4:Table 4:

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript Repräsentative Chromosomale FoId q-value Probennummer ID ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript Representative Chromosomal FoId q-value Sample Number ID ID Protein ID Localization Change (%)

1378 203084_at TGFB1 transforming growth NM_000660 gl 0863872 NP_000651 Chr:19q13.1 1000 2.814919 factor, beta 1 (Camurati-Engelmann disease)1378 203084_at TGFB1 transforming growth NM_000660 gl 0863872 NP_000651 Chr: 19q13.1 1000 2.814919 factor, beta 1 (Camurati-Engelmann disease)

1379 1729_at TRADD TNFRSF1 A-associated L41690 L41690 NP_003780 Chr:16q22 2.559 0.095261 via death domain1379 1729_at TRADD TNFRSF1 A-associated L41690 L41690 NP_003780 Chr: 16q22 2,559 0.095261 via death domain

1380 219304_s_at PDGFD platelet derived growth NM_025208 gl 3376807 NP_079484 Chr:11q22.3 0.49126 6.991358 factor D /// platelet derived growth factor D1380 219304_s_at PDGFD platelet derived growth NM_025208 gl 3376807 NP_079484 Chr: 11q22.3 0.49126 6.991358 factor D /// platelet derived growth factor D

1381 203675_at NUCB2 nucleobindin 2 /// NMJ3O5O13 g4826869 NP_005004 Chr:11p15.1-p14 0.47306 6.991358 nucleobindin 21381 203675_at NUCB2 nucleobindin 2 /// NMJ3O5O13 g4826869 NP_005004 Chr: 11p15.1-p14 0.47306 6.991358 nucleobindin 2

1382 226705_at FGFR1 fibroblast growth factor BE467261 Hs.748.8 NPJ300595 Chr:8p11.2-p11.1 0.46829 0.095261 receptor 1 (fms-related tyrosine kinase 2, Pfeiffer Syndrome)1382 226705_at FGFR1 fibroblast growth factor BE467261 Hs.748.8 NPJ300595 Chr: 8p11.2-p11.1 0.46829 0.095261 receptor 1 (fms-related tyrosine kinase 2, Pfeiffer Syndrome)

1383 226625_at TGFBR3 transforming growth AW193698 Hs.79059.1 NP_003234 Chr:1p33-p32 0.45978 0.095261 factor, beta receptor III (betaglycan, 30OkDa)1383 226625_at TGFBR3 transforming growth AW193698 Hs.79059.1 NP_003234 Chr: 1p33-p32 0.45978 0.095261 factor, beta receptor III (betaglycan, 30OkDa)

1384 205051 s at KIT v-kit Hardy-Zuckerman NM_000222 g4557694 NPJ300213 Chr:4q11-q12 0.45787 4.830969 4 feline sarcoma viral oncogene homolog Ul v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog1384 205051 s at KIT v-kit Hardy-Zuckerman NM_000222 g4557694 NPJ300213 Chr: 4q11-q12 0.45787 4.830969 4 feline sarcoma viral oncogene homolog Ul v kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

1385 203685 at BCL2 B-cell CLL/lymphoma NM_000633 g4557354 NP_000624 Chr:18q21.3 0.44313 0.095261 2 /// B-cell CLL/lymphoma 21385 203685 at BCL2 B-cell CLL / lymphoma NM_000633 g4557354 NP_000624 Chr: 18q21.3 0.44313 0.095261 2 /// B-cell CLL / lymphoma 2

1386 210174 at NR5A2 nuclear receptor AF228413 Hs.183123.1 NP_003813 Chr:1q32.1 0.41209 3.612633 subfamily 5, groυp A, member 2 /// nuclear receptor subfamily 5, group A, member 2 1386 210174 at NR5A2 nuclear receptor AF228413 Hs.183123.1 NP_003813 Chr: 1q32.1 0.41209 3.612633 subfamily 5, groυp A, member 2 /// nuclear receptor subfamily 5, group A, member 2

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript Repräsentative Chromosomale FoId q-value Probennummer ID ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript Representative Chromosomal FoId q-value Sample Number ID ID Protein ID Localization Change (%)

1387 205207_at IL6 interleukiπ 6 NM_000600 gl 0834983 NP_000591 Chr:7p21 0.40071 1.702877 (interferon, beta 2) /// interleukin 6 (interferon, beta 2)1387 205207_at IL6 interleukiπ 6 NM_000600 gl 0834983 NP_000591 Chr: 7p21 0.40071 1.702877 (interferon, beta 2) /// interleukin 6 (interferon, beta 2)

1388 210397_at DEFB1 defensin, beta 1 /// U73945 gl 755147 NP_005209 Chr:8p23.2-p23.1 0.39972 11.06357 defensin, beta 11388 210397_at DEFB1 defensin, beta 1 /// U73945 gl 755147 NP_005209 Chr: 8p23.2-p23.1 0.39972 11.06357 defensin, beta 1

1389 226333_at IL6R interleukin 6 receptor AV700030 Hs.17998.0 NP 000556 Chr:1q21 0.3997 1.0512981389 226333_at IL6R interleukin 6 receptor AV700030 Hs.17998.0 NP 000556 Chr: 1q21 0.3997 1.051298

1390 203178_at GATM glycine NM_001482 g4503932 NP_001473 Chr:15q15.1 0.382 5.685182 amidinotransferase (L- arginine:glycine amidinotransferase)1390 203178_at GATM glycine NM_001482 g4503932 NP_001473 Chr: 15q15.1 0.382 5.685182 amidinotransferase (L-arginine: glycine amidinotransferase)

1391 222043 at CLU clusterin (complement AI982754 Hs.75106.1 NP 001822 Chr:8p21-p12 0.35018 2.571322 lysis inhibitor, SP- 40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J)1391 222043 at CLU clusterin (complement AI982754 Hs.75106.1 NP 001822 Chr: 8p21-p12 0.35018 2.571322 lysis inhibitor, SP-40.40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J)

1392 215395_x_at PRSS3 Protease, serine, 3 U66061 Hs.302180.0 NP_002762 Chr:9p11.2 0.33276 11.06357 (mesotrypsin)1392 215395_x_at PRSS3 protease, serine, 3 U66061 Hs.302180.0 NP_002762 Chr: 9p11.2 0.33276 11.06357 (mesotrypsin)

1393 206010_at HABP2 hyaluronaπ binding NM_004132 g4758501 NP_004123 Chr:10q26.11 0.32304 3.612633 protein 2 /// hyaluronan binding protein 2HABP2 hyaluronan binding NM_004132 g4758501 NP_004123 Chr: 10q26.11 0.32304 3.612633 protein 2 /// hyaluronan binding protein 2

1394 204705_x_at ALDOB aldolase B, fructose- NM_000035 g4557306 NP_000026 Chr:9q21.3-q22.2 0.31788 1.901012 bisphosphate1394 204705_x_at ALDOB aldolase B, fructose NM_000035 g4557306 NP_000026 Chr: 9q21.3-q22.2 0.31788 1.901012 bisphosphates

1395 206492_at FHIT fragile histidine triad NM_002012 g4503718 NP_002003 Chr:3p14.2 0.30534 6.991358 gene /// fragile histidine triad gene1395 206492_at FHIT fragile histidine triad NM_002012 g4503718 NP_002003 Chr: 3p14.2 0.30534 6.991358 gene /// fragile histidine triad gene

1396 207463_x_at PRSS2 Protease, serine, 2 NM_002771 g4506148 NP_002761 Chr:7q34 0.29963 7.646445 (trypsin 2) /// protease, serine, 2 (trypsin 2)1396 207463_x_at PRSS2 protease, serine, 2 NM_002771 g4506148 NP_002761 Chr: 7q34 0.29963 7.646445 (trypsin 2) /// protease, serine, 2 (trypsin 2)

1397 41657_at STK11 serine/threonine AF035625 4862706_RC NP_000446 Chr:19p13.3 0.26727 0.095261 kinase 11 (Peutz- Jeghers Syndrome) 1397 41657_at STK11 serine / threonine AF035625 4862706_RC NP_000446 Chr: 19p13.3 0.26727 0.095261 kinase 11 (Peutz-Jeghers Syndrome)

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript Representative Chromosomal FoId q-value

Probennummer ID ID Protein ID Lokalisierung Change >0'-Sample ID ID ID Protein ID Localization Change> 0 '-

1398 205043 at CFTR cystic fibrosis NM_000492 g6995995 NP_000483 Chr:7q31.2 0.26656 5.685182 transmembrane conductance regulator, ATP-binding cassette (sub-family C, member 7) /// cystic fibrosis transmembrane conductance regulator, ATP-binding cassette (sub-family C, member J)1398 205043 at CFTR cystic fibrosis NM_000492 g6995995 NP_000483 Chr: 7q31.2 0.26656 5.685182 transmembrane conductance regulator, ATP-binding cassette (sub-family C, member 7) /// cystic fibrosis transmembrane conductance regulator, ATP-binding cassette (sub-family C, member J)

1399 204359 at FLRT2 fibronectin leucine rieh NM_013231 g7019380 NP_037363 Chr:14q24-q32 0.25466 4.436744 transmembrane protein 2 /// fibronectin leucine rieh transmembrane protein 21399 204359 at FLRT2 fibronectin leucine rieh NM_013231 g7019380 NP_037363 Chr: 14q24-q32 0.25466 4.436744 transmembrane protein 2 /// fibronectin leucine transmembrane protein 2

1400 203908 at SLC4A4 solute carrier family 4, NM_003759 g4507024 NP_003750 Chr:4q21 0.24611 3.612633 £ c

Figure imgf000117_0001
\ sodium bicarbonate cotransporter, member 4 /// solute carrier family 4, sodium bicarbonate cotransporter, member 41400 203908 at SLC4A4 solute carrier family 4, NM_003759 g4507024 NP_003750 Chr: 4q21 0.24611 3.612633 £ c
Figure imgf000117_0001
\ sodium bicarbonate cotransporter, member 4 /// solute carrier family 4, sodium bicarbonate cotransporter, member 4

1401 213706 at GPD1 glycerol-3-phosphate AI368018 Hs.9739.1 NP_005267 Chr:12q12-q13 0.20425 0.095261 dehydrogenase 1 (soluble)1401 213706 at GPD1 glycerol-3-phosphate AI368018 Hs.9739.1 NP_005267 Chr: 12q12-q13 0.20425 0.095261 dehydrogenase 1 (soluble)

1402 207434 s at FXYD2 FXYD domain NM_021603 gl 1125763 NP_001671 Chr:11q23 0.19347 3.612633 containing ion transport regulator 2 /// FXYD domain containing ion transport regulator 2 FXYD2 FXYD domain NM_021603 gl 1125763 NP_001671 Chr: 11q23 0.19347 3.612633 containing ion transport regulator 2 /// FXYD domain containing ion transport regulator 2

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript Repräsentative Chromosomale FoId q-valueNo. Affymetrix Gene Symbol Gene Name Representative Transcript Representative Chromosomal FoId q-value

Probennummer ID ID Protein ID Lokalisierung Change (%)Sample ID ID ID Protein ID Localization Change (%)

1403 208450 at LGALS2 lectin, galactoside- NM_006498 g5729902 NP_006489 Chr:22q13.1 0.19141 4.436744 binding, soluble, 2 (galectin 2) /// lectin, galactoside-binding, soluble, 2 (galectin 2)1403 208450 at LGALS2 lectin, galactoside NM_006498 g5729902 NP_006489 Chr: 22q13.1 0.19141 4.436744 binding, soluble, 2 (galectin 2) /// lectin, galactoside-binding, soluble, 2 (galectin 2)

1404 214324 at BF222483 Hs.274454.0 0.15667 3.6126331404 214324 at BF222483 Hs.274454.0 0.15667 3.612633

1405 202888 s at ANPEP alanyl (membrane) NM_001150 g4502094 NP_001141 Chr:15q25-q26 0.15368 11.70204 aminopeptidase (aminopeptidase N, aminopeptidase M, microsomal aminopeptidase, CD13, p150) /// alanyl (membrane) aminopeptidase (aminopeptidase N, aminopeptidase M, microsomal aminopeptidase, CD13, p150)1405 202888 s at ANPEP alanyl (membrane) NM_001150 g4502094 NP_001141 Chr: 15q25-q26 0.15368 11.70204 aminopeptidase (aminopeptidase N, aminopeptidase M, microsomal aminopeptidase, CD13, p150) /// alanyl (membrane) aminopeptidase (aminopeptidase N, aminopeptidase M, microsomal aminopeptidase, CD13, p150)

1406 216699 s at KLK1 kallikrein 1, L10038 Hs.123107.1 NP 002248 Chr:19q13.3 0.14433 4.149813 renal/pancreas/salivary1406 216699 s at KLK1 kallikrein 1, L10038 Hs.123107.1 NP 002248 Chr: 19q13.3 0.14433 4.149813 renal / pancreas / salivary

1407 207636 at SERPIN12 serine (or cysteine) NM_006217 gl 0947029 NP_006208 Chr:3q26.1-q26.2 0.14425 4.830969 Proteinase inhibitor, clade I (neuroserpin), member 2 /// serine (or cysteine) Proteinase inhibitor, clade I (neuroserpin), member 21407 207636 at SERPIN12 serine (or cysteine) NM_006217 gl 0947029 NP_006208 Chr: 3q26.1-q26.2 0.14425 4.830969 Proteinase inhibitor, clade I (neuroserpin), member 2 /// serine (or cysteine) Proteinase inhibitor, clade I (neuroserpin ), member 2

1408 206311_s. _at PLA2G1B phospholipase A2, NM. ,000928 g4505846 NP. _000919 Chr:12q23-q24.1 0.13114 3.612633 group IB (pancreas) /// phospholipase A2, group IB (pancreas)1408 206311_s. _at PLA2G1B phospholipase A2, NM. , 000928 g4505846 NP. _000919 Chr: 12q23-q24.1 0.13114 3.612633 group IB (pancreas) /// phospholipase A2, group IB (pancreas)

1409 206446_s. jat ELA2A elastase 2A /// NM. _001971 g4503546 NP. .254275 Chr:1p36.13 0.11261 3.612633 elastase 2A 1409 206446_s. jat ELA2A elastase 2A /// NM. _001971 g4503546 NP. , 254275 Chr: 1p36.13 0.11261 3.612633 elastase 2A

Nr. Affymetrix Gensymbol Genname Repräsentative Transkript Repräsentative Chromosomale FoId q-value Probennummer ID ID Protein ID Lokalisierung Change (%)No. Affymetrix Gene Symbol Gene Name Representative Transcript Representative Chromosomal FoId q-value Sample Number ID ID Protein ID Localization Change (%)

1410 206151_x_at ELA3B elastase 3B, NM_007352 g6679624 NP_031378 Chr:1p36.12 0.1018 0.095261 pancreatic /// elastase 3B, pancreatic1410 206151_x_at ELA3B elastase 3B, NM_007352 g6679624 NP_031378 Chr: 1p36.12 0.1018 0.095261 pancreatic /// elastase 3B, pancreatic

1411 203924_at GSTA1 glutathione S- NM_000846 g4504170 NP_665683 Chr:6p12.1 0.09258 3.612633 transferase A1 /// glutathione S- transferase A11411 203924_at GSTA1 glutathione S-NM_000846 g4504170 NP_665683 Chr: 6p12.1 0.09258 3.612633 transferase A1 /// glutathione S-transferase A1

1412 206694_at PNLIPRP1 pancreatic lipase- NM_006229 g5453919 NP_006220 Chr:10q26.12 0.0795 3.612633 related protein 1 /// pancreatic lipase- related protein 11412 206694_at PNLIPRP1 pancreatic lipase NM_006229 g5453919 NP_006220 Chr: 10q26.12 0.0795 3.612633 related protein 1 /// pancreatic lipase-related protein 1

1413 214377_s_at CTRL chymotrypsin-like BF508685 Hs.150601.2 NP 001898 Chr:16q22.1 0.06878 8.7793571413 214377_s_at CTRL chymotrypsin-like BF508685 Hs.150601.2 NP 001898 Chr: 16q22.1 0.06878 8.779357

1414 205971 _s_at CTRB1 chymotrypsinogen B1 NM_001906 g4503134 NP_001897 Chr:16q23-q24.1 0.06862 4.436744 /// chymotrypsinogen B11414 205971 _s_at CTRB1 chymotrypsinogen B1 NM_001906 g4503134 NP_001897 Chr: 16q23-q24.1 0.06862 4.436744 /// chymotrypsinogen B1

1415 205615_at CPA1 carboxypeptidase A1 NM_001868 g4502996 NP_001859 Chr:7q32 0.06354 6.225758 (pancreatic) /// carboxypeptidase A1 (pancreatic)1415 205615_at CPA1 carboxypeptidase A1 NM_001868 g4502996 NP_001859 Chr: 7q32 0.06354 6.225758 (pancreatic) /// carboxypeptidase A1 (pancreatic)

1416 227238_at MUC15 mucin 15 W93847 Hs.24139.0 NP 663625 Chr:11p14.3 0.06055 9.2667771416 227238_at MUC15 mucin 15 W93847 Hs.24139.0 NP 663625 Chr: 11p14.3 0.06055 9.266777

1417 210168_at C6 complement J05064 gl 79703 NP_000056 Chr:5p13 0.04412 1.578435 component 6 /// complement component 61417 210168_at C6 complement J05064 gl 79703 NP_000056 Chr: 5p13 0.04412 1.578435 component 6 /// complement component 6

1418 206131_at CLPS colipase, pancreatic /// NM_001832 gl 1496883 NP_001823 Chr:6pter-p21.1 0.0371 4.830969 colipase, pancreatic1418 206131_at CLPS colipase, pancreatic /// NM_001832 gl 1496883 NP_001823 Chr: 6pter-p21.1 0.0371 4.830969 colipase, pancreatic

1419 205910 s at CEL carboxyl ester lipase NM_001807 g4502770 NP_001798 Chr:9q34.3 0.03629 3.612633 (bile salt-stimulated lipase) /// carboxyl ester lipase (bile salt- stimulated lipase) Biol salt-stimulated lipase

In einer bevorzugten Ausführungsform des erfindungsgemäßen Verfahrens erfolgt zusätzlich zur Bestimmung der Menge an Genprodukt von mindestens einem der in Tabelle 1 und / oder Tabelle 2 aufgeführten Gene in einer Probe biologischen Ursprungs eines Individuums noch die Bestimmung der Menge an Genprodukt von mindestens einem der in Tabelle 3 und/oder Tabelle 4 aufgeführten Gene. Die Menge an Genprodukt in der Probe biologischen Ursprungs eines Individuums wird mit der Menge des entsprechenden Genprodukts, die in normalem Pankreasepithelgewebe üblicherweise vorliegt, verglichen. Falls es sich um ein in der Tabelle 3 aufgeführtes Gen handelt, ist eine erhöhte Menge des Genprodukt ein Indiz für das Vorliegen eines Pankreaskarzioms. Falls es sich um ein in der Tabelle 4 aufgeführtes Gen handelt, ist eine erniedrigte Menge des Genprodukts ein Indiz für das Vorliegen eines Pankreaskarzinoms.In a preferred embodiment of the method according to the invention, in addition to determining the amount of gene product of at least one of the genes listed in Table 1 and / or Table 2 in a sample of biological origin of an individual nor the determination of the amount of gene product of at least one of the in Table 3 and / or Table 4 genes. The amount of gene product in the sample of biological origin of an individual is compared to the amount of the corresponding gene product commonly present in normal pancreatic epithelial tissue. If it is a gene listed in Table 3, an increased amount of the gene product is an indication of the presence of a pancreatic carcinoma. If it is a gene listed in Table 4, a decreased amount of the gene product is an indication of the presence of a pancreatic carcinoma.

Durch die Messung eines solchen zusätzlichen Markers (Gene aus Tabelle 3 und/oder Tabelle 4) wird die Aussagekraft des erfindungsgemäßen Verfahrens noch weiter erhöht.By measuring such an additional marker (genes from Table 3 and / or Table 4), the significance of the method according to the invention is further increased.

Ein Genprodukt ist eine mRNA, die daraus durch reverse Transkription erhältliche cDNA, das kodierte Protein oder Fragmente davon. Die Quantifizierung der Genprodukte erfolgt dabei jeweils mit den gängigen Verfahren.A gene product is an mRNA, the cDNA obtainable therefrom by reverse transcription, the encoded protein or fragments thereof. The quantification of the gene products is carried out in each case with the usual methods.

Die Genprodukte mRNA bzw. die durch reverse Transkription der mRNA erhältliche cDNA werden bevorzugt durch den Einsatz von Hybridisierungstechniken quantifiziert, beispielsweise durch einen Northern Blot, in situ Hybridisierung mit antisense-RNA, quantitative PCR (Polymerase chain reactiori) oder mit Hilfe eines Oligonukleotid- oder eines cDNA-Mikroarrays (z.B. DNA-Chip).The gene products mRNA or the cDNA obtainable by reverse transcription of the mRNA are preferably quantified by the use of hybridization techniques, for example by a Northern blot, in situ hybridization with antisense RNA, quantitative PCR (polymerase chain reactiori) or by means of an oligonucleotide or a cDNA microarray (eg DNA chip).

Die Oligonukleotide, die für die verschiedenen Nachweistechniken mittels Hybridisierung eingesetzt werden, haben Sequenzen, die komplementär zu Teilsequenzen des zu detektierenden Genprodukts sind, und sind vorzugsweise mit einem chromogenen, radioaktiven, fluoreszierenden oder durch andere gängige Methoden detektierbaren Markermolekül markiert. Beispiele für solche Markermoleküle sind Digoxygenin, Biotin, Fluorescein, 32P, 33P, usw.The oligonucleotides used for the different detection techniques by hybridization have sequences that are complementary to partial sequences of the gene product to be detected, and are preferably labeled with a chromogenic, radioactive, fluorescent or by other current methods detectable marker molecule. Examples of such marker molecules are digoxygenin, biotin, fluorescein, 32 P, 33 P, etc.

Bevorzugt erfolgt die quantitative Messung der Expression des Genprodukts mRNA mittels quantitativer RT-PCR. In dem bekannten Prozess der RT-PCR wird zunächst mit Hilfe des Enzyms Reverse Transkriptase (RT) basierend auf der isolierten RNA komplementäre DNA (cDNA) synthetisiert. Als Primer für die RT kann grundsätzlich ein Oligo-dT verwendet werden. Die Verwendung von Random-Hexamer-Primern für die RT führt jedoch zu einer deutlichen Erhöhung der Sensitivität. Das Random-Hexamer-Oligonukleotid enthält jeweils eine Mischung von A, G, T, C an allen sechs Positionen und hybridisiert unspezifisch mit mRNA-Sequenzen.The quantitative measurement of the expression of the gene product mRNA preferably takes place by means of quantitative RT-PCR. In the known process of RT-PCR, first complementary DNA (cDNA) is synthesized based on the isolated RNA using the enzyme reverse transcriptase (RT). In principle, an oligo-dT can be used as primer for the RT. However, the use of random hexamer primers for the RT leads to a significant increase in sensitivity. The random hexamer oligonucleotide each contains a mixture of A, G, T, C at all six positions and hybridizes nonspecifically with mRNA sequences.

Einzelne cDNAs werden in der anschließenden PCR mit einem sequenzspezifischen Primerpaar amplifiziert. Die Primer für die PCR haben bevorzugt eine Länge von 15 bis 30 Basenpaaren und werden Intron-überlappend konzipiert, um im Falle einer Verunreinigung der RNA mit genomischer DNA nur cDNA zu amplifizieren.Individual cDNAs are amplified in the subsequent PCR with a sequence-specific primer pair. The primers for the PCR preferably have a length of 15 to 30 base pairs and are designed to be intron-overlapping in order to amplify only cDNA in the event of contamination of the RNA with genomic DNA.

Besonders bevorzugt wird die PCR als Real-time-PCR durchgeführt. Bekannte Real-time PCR Verfahren sind z. B. die TaqMan®, FRET (Fluoreszenz-Resonanz-Energie-Transfer), LightCycler® und Beacon-Verfahren. Durch die Verwendung von Fluoreszenz-markierten Primern oder den Einsatz von genspezifischen Oligonukleotiden als Hybridisierungsproben mit unterschiedlicher Farbstoff- oder Fluoreszenzmarker-Anbindung (TaqMan®, FRET, Beacon) kann bei diesen Verfahren vorteilhaft das PCR-Produkt während der PCR spezifisch quantifiziert werden.The PCR is particularly preferably carried out as a real-time PCR. Known real-time PCR methods are z. Such as the TaqMan®, FRET (Fluorescence Resonance Energy Transfer), LightCycler® and Beacon methods. By using fluorescence-labeled primers or the use of gene-specific oligonucleotides as hybridization probes with different dye or fluorescent marker binding (TaqMan®, FRET, Beacon), the PCR product can be specifically quantified during the PCR in these methods advantageously.

Die PCR-Verfahren eignen sich auch zur Durchführung als Multiplex-PCR. Bei einer Multiplex-PCR unterscheiden sich die Fluoreszenzfarbstoffe, mit denen die Primer bzw. Hybridisierungssonden markiert sind, in ihren Absorptions- und/oder Emissionsspektren. Dies ermöglicht beispielsweise eine parallele Quantifizierung der amplifizierten cDNAs verschiedener pankreastumorspezifischen Gene bzw. die parallele Quantifizierung der cDNA eines Referenzgens bzw. einer Referenzprobe.The PCR methods are also suitable for use as multiplex PCR. In a multiplex PCR, the fluorescent dyes with which the primers or hybridization probes are labeled differ in their absorption and / or emission spectra. This allows, for example, a parallel quantification of the amplified cDNAs of various pancreatic tumor-specific genes or the parallel quantification of the cDNA of a reference gene or a reference sample.

Zur Durchführung des erfindungsgemäßen Verfahrens zur Diagnose mittels RT-PCR wird bevorzugt ein diagnostischer Kit verwendet, der jeweils folgende Bestandteile enthält:For carrying out the method according to the invention for the diagnosis by means of RT-PCR, a diagnostic kit which contains in each case the following components is preferably used:

1 ) für die cDNA-Synthese: a) Random-Hexamer-Primer, b) Reverse Transkriptase;1) for cDNA synthesis: a) random hexamer primer, b) reverse transcriptase;

2) für die PCR: a) mindestens zwei Primer, die mit der cDNA eines der Gene, ausgewählt aus den Gruppen der tumorspezifischen Gene aus den Tabellen Nr. 1 und/oder Nr. 2 hybridisieren; b) eine über 80°C beständige DNA-Polymerase, wie z. B. Taq-Polymerase; sowie dNTP-Mix (bevorzugt 10mmol/l), DDT, RNase-Inhibitor und entsprechende Reaktionspuffer.2) for the PCR: a) at least two primers which hybridize with the cDNA of one of the genes selected from the groups of the tumor-specific genes from Tables No. 1 and / or No. 2; b) a more than 80 ° C resistant DNA polymerase such. B. Taq polymerase; and dNTP mix (preferably 10 mmol / l), DDT, RNase inhibitor and corresponding reaction buffer.

In einer weniger bevorzugten Ausführungsform enthält der Kit für die cDNA-Synthese oligo- dT anstelle des Random-Hexamers.In a less preferred embodiment, the kit for cDNA synthesis contains oligod-d instead of the random hexamer.

In einer alternativen Ausführungsform enthält das Kit zusätzlich mindestens zwei Primer, die mit der cDNA eines der Gene, ausgewählt aus den Gruppen der tumorspezifischen Gene aus den Tabellen 3 und 4 hybridisieren.In an alternative embodiment, the kit additionally contains at least two primers which hybridize with the cDNA of one of the genes selected from the groups of the tumor-specific genes from Tables 3 and 4.

In einer Ausführungsform des Kits ist jeweils einer der Primer für das tumorspezifische Gen Fluoreszenz-markiert.In one embodiment of the kit, in each case one of the primers for the tumor-specific gene is fluorescence-labeled.

In einer besonders bevorzugten Ausführungsform enthält der diagnostische Kit zur Durchführung einer Real-Time PCR bevorzugt als zusätzliche Bestandteile: c) eine Fluoreszenz-markierte Hybridisierungssonde oder ein Paar Fluoreszenz¬ markierter Hybridisierungssonden, die spezifisch mit einem tumorspezifischen Gen aus Tabelle 1 und/oder Tabelle 2 hybridisieren, und d) eine Fluoreszenz-markierte Hybridisierungssonde oder ein Paar Fluoreszenz¬ markierter Hybridisierungssonden, die spezifisch mit dem Referenzgen hybridisieren.In a particularly preferred embodiment, the diagnostic kit for carrying out a real-time PCR preferably contains as additional constituents: c) a fluorescence-labeled hybridization probe or a pair of fluorescence-labeled hybridization probes which are specific with a tumor-specific gene from Table 1 and / or Table 2 and d) a fluorescence-labeled hybridization probe or a pair of fluorescence-labeled hybridization probes that specifically hybridize to the reference gene.

Sowohl als Primer, als auch als Hybridisierungssonden werden bevorzugt DNA- Oligonukleotide mit einer Länge von 10 bis 30 Nukleotiden gewählt.Both as primers, as well as hybridization probes DNA oligonucleotides are preferably selected with a length of 10 to 30 nucleotides.

In einer Ausführungsform enthält die Hybridisierungssonde neben dem Fluoreszenzfarbstoff einen weiteren Farbstoff, der durch einen Fluoreszenz-Resonanz-Energie-Transfer (FRET) die Fluoreszenz quencht, solange die Probe nicht mit der amplifizierten cDNA hybridisiert.In one embodiment, the hybridization probe contains, in addition to the fluorescent dye, another dye that quenches the fluorescence by fluorescence resonance energy transfer (FRET) as long as the sample does not hybridize with the amplified cDNA.

In einer alternativen Ausführungsform des Kits werden zwei Hybridisierungssonden eingesetzt, bei denen jeweils eine Sonde mit einem Farbstoff markiert ist. Ein solches Sondenpaar wird so gewählt, dass sie in einem geringen Abstand, bevorzugt im Abstand von 1-5 bp, auf der amplifizierten cDNA hybridisieren. Durch die Hybridisierung werden die Fluoreszenzfarbstoffe so nahe zueinander gebracht, dass FRET stattfindet und die cDNA quantifiziert werden kann. Anschließend werden (in der Extensionsphase der PCR), durch die Exonuklease-Aktivität der Polymerase, die Sonden von der cDNA abgetrennt. In einer bevorzugten Variante ist in einem Sondenpaar, die erste Sonde am 3'-Ende mit Fluorescein (FL) und die andere an ihrem 5'-Ende mit LightCycler-Red-640 markiert. Fluoreszenz-markierte Hybridisierungssonden sind z. B. als LightCycler®, TaqMan® oder „molecular beacons" auf dem Markt und ermöglichen eine Quantifizierung während der PCR.In an alternative embodiment of the kit, two hybridization probes are used, each labeled with a dye. Such a probe pair is chosen so that they hybridize at a small distance, preferably at a distance of 1-5 bp, on the amplified cDNA. By hybridization, the fluorescent dyes are brought so close to each other that FRET takes place and the cDNA can be quantified. Subsequently (in the extension phase of the PCR), the probes are separated from the cDNA by the exonuclease activity of the polymerase. In a preferred variant, in one probe pair, the first probe at the 3 'end is labeled with fluorescein (FL) and the other at its 5' end labeled with LightCycler Red 640. Fluorescence-labeled hybridization probes are e.g. As LightCycler®, TaqMan® or "molecular beacons" on the market and allow quantification during PCR.

Der Nachweis über Hybridisierung erfolgt in einer alternativen Ausführung des Verfahrens unter Verwendung eines Nukleinsäure-Arrays, z.B. eines DNA-Chips.Evidence of hybridization is provided in an alternative embodiment of the method using a nucleic acid array, e.g. a DNA chip.

DNA-Chips, auch als DNA-Mikroarrays bekannt, sind miniaturisierte Träger, meist aus Glas oder Silizium, auf deren Oberfläche DNA-Moleküle bekannter Sequenz in einem geordneten Raster in hoher Dichte immobilisiert werden. Die oberflächengebundenen DNA-Moleküle werden mit komplementären, eventuell markierten Nukleinsäuren hybridisiert. Die Markierung ist vorzugsweise ein Fluoreszenzfarbstoff.DNA chips, also known as DNA microarrays, are miniaturized carriers, usually made of glass or silicon, on the surface of which DNA molecules of known sequence are immobilized in an ordered grid in high density. The surface-bound DNA molecules are hybridized with complementary, possibly labeled nucleic acids. The label is preferably a fluorescent dye.

Bei Oligonukleotid-Chips stellen die Oligonukleotide, die auf einem erfindungsgemäßen DNA-Chip gebunden sein können, Teilsequenzen der Genprodukte (mRNA, bzw. daraus abgeleitete cDNA) in der sense- oder antisense-Richtung dar. Es können ein oder mehrere Oligonukleotide pro Gen auf dem DNA-Chip gebunden sein. Bevorzugt sind 25 Nukleotid lange Oligonukleotide, die aus dem nicht kodierenden Strang abgeleitet sind. Diese werden bevorzugt aus dem jeweiligen 3' untranslatierten Ende des Gens ausgewählt. Zur Detektion können Oligonukleotide von einem Gen, mehreren Genen oder allen Genen ausgewählt werden.In the case of oligonucleotide chips, the oligonucleotides, which may be bound to a DNA chip according to the invention, are partial sequences of the gene products (mRNA, or cDNA derived therefrom) in the sense or antisense direction. One or more oligonucleotides may be present per gene be bound to the DNA chip. Preferred are 25 nucleotide oligonucleotides derived from the non-coding strand. These are preferably selected from the respective 3 'untranslated end of the gene. For detection, oligonucleotides can be selected from one gene, several genes or all genes.

Die Erfindung betrifft daher weiterhin die Verwendung mindestens eines Oligonukleotids, das der vollständigen cDNA-Sequenz oder einer Teilsequenz bzw. komplementären Sequenz eines der in Tabelle 1 und/oder 2 aufgeführten Gene entspricht, zur Diagnoses des Pankreaskarzinoms, insbesondere des duktalen Adenomakarzinoms des Pankreas. Das Oligonukleotid kann dazu als Primer in einer PCR oder als Hybridisierungssonde eingesetzt werden. Das verwendete Oligonukleotid bzw. die verwendeten Oligonukleotide sind dabei vorzugsweise auf einem festen Träger (DNA-Chip) immobilisiert.The invention therefore furthermore relates to the use of at least one oligonucleotide which corresponds to the complete cDNA sequence or a partial sequence or complementary sequence of one of the genes listed in Table 1 and / or 2 for the diagnosis of pancreatic carcinoma, in particular ductal adenoma carcinoma of the pancreas. The oligonucleotide can be used as a primer in a PCR or as a hybridization probe. The oligonucleotide used or the oligonucleotides used are preferably immobilized on a solid support (DNA chip).

In einer alternativen Ausführungsform wird zusätzlich mindestens ein Oligonukleotid, das der vollständigen cDNA-Sequenz oder einer Teilsequenz bzw. komplementären Sequenz eines der in Tabelle 3 und/oder 4 aufgeführten Gene entspricht, zur Diagnoses des Pankreaskarzinoms, insbesondere des duktalen Adenomakarzinoms des Pankreas verwendet. Das verwendete Oligonukleotid bzw. die verwendeten Oligonukleotide sind dabei vorzugsweise auf einem Chip immobilisiert. Ist das zu quantifizierende Genprodukt ein Protein oder ein Proteinfragment, erfolgt die Bestimmung der Menge an Genprodukt vorzugsweise durch Antikörper-basierte Nachweismethoden.In an alternative embodiment, additionally at least one oligonucleotide which corresponds to the complete cDNA sequence or a partial sequence or complementary sequence of one of the genes listed in Table 3 and / or 4 is used for the diagnosis of pancreatic carcinoma, in particular ductal adenoma carcinoma of the pancreas. The oligonucleotide used or the oligonucleotides used are preferably immobilized on a chip. If the gene product to be quantified is a protein or a protein fragment, the amount of gene product is preferably determined by antibody-based detection methods.

Dazu werden spezifische Antikörper gegen eines oder mehrere Proteine, die von den in Tabelle 1 und/oder Tabelle 2 aufgeführten Genen kodiert werden, hergestellt. In einer alternativen Ausführungsform werden zusätzlich Antikörper gegen eines oder mehrere Proteine, die von den in Tabelle 3 und/oder Tabelle 4 aufgeführten Genen kodiert werden, eingesetzt.For this purpose, specific antibodies against one or more proteins, which are encoded by the genes listed in Table 1 and / or Table 2, prepared. In an alternative embodiment, antibodies are additionally used against one or more proteins which are encoded by the genes listed in Table 3 and / or Table 4.

Bei den Antikörpern handelt es sich beispielsweise um monoklonale Antikörper, polyklonale Antikörper, Single chain Antikörper oder Fragmente davon. Sie sind spezifisch für das gesamte Protein oder nur gegen Fragmente davon. Die Gewinnung eines solchen Antikörpers erfolgt nach Standardmethoden beispielsweise durch Immunisierung von Versuchstieren. Die Antikörper werden dann z.B. in einem ELISA (enzyme linked immunsorbent assay), in einem RIA (radio-immunoassay) oder in der Immunhistochemie zur Quantifizierung des Genprodukts verwendet.The antibodies are, for example, monoclonal antibodies, polyclonal antibodies, single-chain antibodies or fragments thereof. They are specific to the entire protein or only to fragments thereof. The recovery of such an antibody is carried out according to standard methods, for example by immunization of experimental animals. The antibodies are then added e.g. used in an enzyme linked immunosorbent assay (ELISA), in a radioimmunoassay (RIA) or in immunohistochemistry for quantification of the gene product.

Ein Antikörper-Array bzw. Antikörperchip, auf dessen Oberfläche mindestens ein Antikörper, der spezifisch für ein von den in Tabelle 1 und/oder Tabelle 2 aufgeführten Genen kodiertes Protein ist, in einem geordneten Rahmen in hoher Dichte immobilisiert ist, eignet sich ebenfalls zur erfindungsgemäßen Diagnose des Pankreaskarzinoms. Die Dektektion der Protein-Protein-Interaktion erfolgt vorzugsweise durch Massenspektrometrie, Fluoreszenz oder surface plasmon resonance.An antibody array or antibody chip, on the surface of which at least one antibody which is specific for a protein encoded by the genes listed in Table 1 and / or Table 2, is immobilized in an ordered frame in high density, is likewise suitable for the invention Diagnosis of pancreatic carcinoma. The detection of the protein-protein interaction is preferably carried out by mass spectrometry, fluorescence or surface plasmon resonance.

Die Erfindung betrifft ferner die Verwendung eines erfindungsgemäßen Antikörper-Chips zur Diagnose des Pankreaskarzinoms.The invention further relates to the use of an antibody chip according to the invention for the diagnosis of pancreatic carcinoma.

Das erfindungsgemäße Verfahren eignet sich auch zur Beurteilung des Krankheitsverlaufs, um z.B. den Erfolg einer Therapie zu bestimmen. In diesem Fall wird die Patientenprobe mit einer älteren Probe des Patienten verglichen.The method according to the invention is also suitable for assessing the course of the disease, e.g. determine the success of a therapy. In this case, the patient sample is compared to an older sample from the patient.

Ein anderer Aspekt der Erfindung betrifft ein Verfahren zum Screenen nach Wirkstoffen, die zur Therapie des Pankreaskarzinoms, insbesondere des duktalen Adenomakarzinoms des Pankreas, geeignet sind. Dabei wird beispielsweise eine Zelllinie eines pankreatischen duktalen Adenokarzinoms mit einer zu testenden Substanz kontaktiert. Anschließend wird die Menge an Genprodukt von mindestens einem der in Tabelle 1 und/oder Tabelle 2 aufgeführten Gene bestimmt. Die Menge an Genprodukt wird dann mit der Menge des entsprechenden Genprodukts, die in einer entsprechenden nicht mit der Testsubstanz kontaktierten Zelllinie vorliegt, verglichen.Another aspect of the invention relates to a method for screening for agents which are suitable for the therapy of pancreatic carcinoma, in particular of the ductal adenoma carcinoma of the pancreas. For example, a cell line of a pancreatic ductal adenocarcinoma is contacted with a substance to be tested. Subsequently, the amount of gene product of at least one of the genes listed in Table 1 and / or Table 2 is determined. The amount of gene product is then compared with the amount of the corresponding gene product present in a corresponding cell line not contacted with the test substance.

Falls es sich um ein in Tabelle 1 aufgeführtes Genprodukt handelt, ist eine gegenüber der unbehandelten Zelllinie erniedrigte Menge des Genprodukts ein Hinweis darauf, dass die Testsubstanz ein potenzieller Wirkstoff für die Therapie des Pankreaskarzinoms ist. Falls es sich um ein in Tabelle 2 aufgeführtes Genprodukt handelt, ist eine gegenüber der unbehandelten Zelllinie erhöhte Menge des Genprodukts ein Hinweis darauf, dass die Testsubstanz ein potenzieller Wirkstoff für die Therapie des Pankreaskarzinoms ist.In the case of a gene product listed in Table 1, an amount of the gene product that is lower than the untreated cell line is an indication that the test substance is a potential drug for the treatment of pancreatic carcinoma. If it is a gene product listed in Table 2, an increased amount of the gene product compared to the untreated cell line is an indication that the test substance is a potential drug for the treatment of pancreatic carcinoma.

In einer alternativen Ausführungsform des Screeningverfahrens werden zusätzlich zur Expression von mindestens einem der in Tabelle 1 und/oder Tabelle 2 aufgeführten Gene die Menge an Genprodukt von mindestens einem der in Tabelle 3 und/oder Tabelle 4 aufgeführten Gene bestimmt.In an alternative embodiment of the screening method, in addition to the expression of at least one of the genes listed in Table 1 and / or Table 2, the amount of gene product of at least one of the genes listed in Table 3 and / or Table 4 is determined.

Falls es sich um ein in Tabelle 3 aufgeführtes Genprodukt handelt, ist eine gegenüber der unbehandelten Zelllinie erniedrigte Menge des Genprodukts ein Hinweis darauf, dass die Testsubstanz ein potenzieller Wirkstoff für die Therapie des Pankreaskarzinoms ist. Falls es sich um ein in Tabelle 4 aufgeführtes Genprodukt handelt, ist eine gegenüber der unbehandelten Zelllinie erhöhte Menge des Genprodukts ein Hinweis darauf, dass die Testsubstanz ein potenzieller Wirkstoff für die Therapie des Pankreaskarzinoms ist.In the case of a gene product listed in Table 3, an amount of the gene product that is reduced from the untreated cell line is an indication that the test substance is a potential drug for the treatment of pancreatic carcinoma. If it is a gene product listed in Table 4, an increased amount of the gene product compared to the untreated cell line is an indication that the test substance is a potential drug for the treatment of pancreatic carcinoma.

Die Erfindung betrifft zudem die Verwendung mindestens eines Antisense-Konstrukts, das mindestens 12 Nukleotide einer Sequenz eines Gens umfasst, das in Tabelle 1 aufgeführt ist, wobei die Sequenz bezüglich des Promotors, der ihre Expression kontrolliert, in 3' zu 5' Orientierung vorliegt, für die Herstellung einer pharmazeutischen Zusammensetzung zur Behandlung des Pankreaskarzinoms beim Menschen, wobei das Antisense-Produkt den Krebszellen verabreicht wird, so dass das Antisense-Konstrukt in den Krebszellen exprimiert wird.The invention further relates to the use of at least one antisense construct comprising at least 12 nucleotides of a sequence of a gene listed in Table 1, the sequence being in 3 'to 5' orientation with respect to the promoter which controls its expression, for the preparation of a pharmaceutical composition for the treatment of human pancreatic carcinoma, wherein the antisense product is administered to the cancer cells such that the antisense construct is expressed in the cancer cells.

Unter Antisense-Konstrukt wird dabei eine Sequenz verstanden, die zu der Sequenz oder einer Teilsequenz der Sequenz komplementär ist, d. h. in der Lage ist mit der Sequenz über Basenpaarung einen Nukleinsäuredoppelstrang zu bilden. In einer alternativen Ausführungsform wird zusätzlich mindestens ein Antisense-Konstrukt, das mindestens 12 Nukleotide einer Sequenz eines Gens umfasst, das in Tabelle 3 aufgeführt ist, wobei die Sequenz bezüglich des Promotors, der ihre Expression kontrolliert, in 3' zu 5' Orientierung vorliegt, für die Herstellung einer pharmazeutischen Zusammensetzung zur Behandlung des Pankreaskarzinoms beim Menschen verwendet, wobei das Antisense-Produkt den Krebszellen verabreicht wird, so dass das Antisense- Konstrukt in den Krebszellen exprimiert wird.By antisense construct is meant a sequence which is complementary to the sequence or a subsequence of the sequence, ie is able to form a nucleic acid double strand with the sequence via base pairing. In an alternative embodiment, additionally at least one antisense construct comprising at least 12 nucleotides of a sequence of a gene listed in Table 3, wherein the sequence is in 3 'to 5' orientation with respect to the promoter which controls its expression, used for the preparation of a pharmaceutical composition for the treatment of human pancreatic carcinoma, wherein the antisense product is administered to the cancerous cells so that the antisense construct is expressed in the cancerous cells.

Das Antisense-Konstrukt wird vorzugsweise durch chemische Synthese, auch unter Verwendung von stabilisierenden Nukleotidanaloga, gewonnen.The antisense construct is preferably obtained by chemical synthesis, also using stabilizing nucleotide analogs.

Teil der Erfindung ist ebenfalls die Verwendung mindestens eines doppelsträngigen RNA- Konstrukts (siRNA - small interfering RNA), das mindestens 19 Basenpaare, besonders bevorzugt 21 oder 22 Basenpaare, maximal 29 Basenpaare, einer Sequenz eines Gens umfasst, das in Tabelle 1 aufgeführt ist, für die Herstellung eines Arzneimittels zur Behandlung des Pankreaskarzinoms beim Menschen. Die doppelsträngige RNA wird z.B. chemisch hergestellt oder mit bekannten biologische Verfahren, beispielsweise in vitro Transkription, den Einsatz von siRNA-Expressionvektoren oder PCR-Expressionskassetten.Part of the invention is also the use of at least one double-stranded RNA construct (siRNA) comprising at least 19 base pairs, more preferably 21 or 22 base pairs, a maximum of 29 base pairs, of a sequence of a gene listed in Table 1, for the manufacture of a medicament for the treatment of pancreatic carcinoma in humans. The double-stranded RNA is e.g. chemically produced or by known biological methods, for example in vitro transcription, the use of siRNA expression vectors or PCR expression cassettes.

In einem bevorzugten Verfahren wird dazu beispielsweise ein cDNA Klon verwendet. Aus diesem wird mittels PCR ein in Tabelle 1 aufgeführtes Gen vervielfältigt und dann durch eine in vitro Transkription doppelsträngige RNA (dsRNA) hergestellt. Diese dsRNA wird dann mit dem Enzym Dicer verdaut. Dabei entstehen die für die Wirkung notwendigen kleinen Stücke in einer Länge von z.B. 21 bp. Das entstandene Gemisch wird dann zur Therapie eingesetzt. Dabei werden die siRNA Moleküle beispielsweise intravenös dem Patienten zugeführt. Die siRNA wirken hierbei gegen die Expression der im Pankreaskarzinom spezifisch überexprimierten Gene.In a preferred method, for example, a cDNA clone is used for this purpose. From this, a gene listed in Table 1 is amplified by means of PCR and then produced by an in vitro transcription double-stranded RNA (dsRNA). This dsRNA is then digested with the enzyme Dicer. At this time, the small pieces necessary for the action are formed in a length of e.g. 21 bp. The resulting mixture is then used for therapy. The siRNA molecules are, for example, administered intravenously to the patient. The siRNA act here against the expression of pancreatic carcinoma specifically overexpressed genes.

In einer besonders bevorzugten Ausführungsform der Erfindung werden doppelsträngigeIn a particularly preferred embodiment of the invention are double-stranded

RNA-Konstrukte für die Herstellung eines Arzneimittels zur Behandlung desRNA constructs for the manufacture of a medicament for the treatment of

Pankreaskarzinoms eingesetzt, die eine Sequenz ausgewählt aus einem der folgendenPancreatic carcinoma used a sequence selected from one of the following

Gene umfassen:Genes include:

APLP2(Nr. 232, Tabelle 1 , Affymetrix-Probennummer 214875_x_at, Genname amyloid betaAPLP2 (# 232, Table 1, Affymetrix sample number 214875_x_at, gene name amyloid beta

(A4) precursor-like protein 2),(A4) precursor-like protein 2),

ECT2 (Nr. 506, Tabelle 1 , Affymetrix-Probennummer 219787_s_at, Genname epithelial cell transforming sequence 2 oncogene), FLJ11323 (Nr. 311 , Tabelle 1 , Affymetrix-Probennummer 218951_s_at, Genname hypothetical protein FLJ11323 NM_018390),ECT2 (# 506, Table 1, Affymetrix sample number 219787_s_at, epithelial cell transforming sequence 2 oncogene gene name), FLJ11323 (# 311, Table 1, Affymetrix Sample No. 218951_s_at, Genname hypothetical protein FLJ11323 NM_018390),

TTK (Nr. 103, Tabelle 1 , Affymetrix-Probennummer 204822_at, Genname TTK protein kinase /// TTK protein kinase).TTK (# 103, Table 1, Affymetrix sample number 204822_at, gene name TTK protein kinase /// TTK protein kinase).

In einer alternativen Ausführungsform wird zusätzlich mindestens ein doppelsträngiges RNA- Konstrukt, das mindestens 19 Basenpaare, besonders bevorzugt 21 oder 22 Basenpaare, maximal 29 Basenpaare, einer Sequenz eines Gens umfasst, das in Tabelle 3 aufgeführt ist, für die Herstellung eines Arzneimittels zur Behandlung des Pankreaskarzioms beim Menschen verwendet. Die doppelsträngige RNA ist dabei z.B. chemisch herstellbar oder über bekannte biologische Verfahren. Dazu wird beispielsweise ein cDNA Klon verwendet. Aus diesem wird mittels PCR ein in Tabelle 3 aufgeführtes Gen vervielfältigt und dann durch eine in vitro Transkription dsRNA hergestellt. Diese dsRNA wird dann mit dem Enzym Dicer verdaut. Dabei entstehen die für die Wirkung notwendigen kleinen Stücke in einer Länge von z.B. 21 bp. Das entstandene Gemisch wird dann zur Therapie eingesetzt.In an alternative embodiment, at least one double-stranded RNA construct comprising at least 19 base pairs, more preferably 21 or 22 base pairs, at most 29 base pairs, of a sequence of a gene listed in Table 3 is additionally used for the manufacture of a medicament for the treatment of Pancreatic carcinoma used in humans. The double-stranded RNA is e.g. Chemically produced or known biological processes. For this purpose, for example, a cDNA clone is used. From this, a gene listed in Table 3 is amplified by PCR and then prepared by an in vitro transcription dsRNA. This dsRNA is then digested with the enzyme Dicer. At this time, the small pieces necessary for the action are formed in a length of e.g. 21 bp. The resulting mixture is then used for therapy.

In einer besonders bevorzugten Ausführungsform der Erfindung werden zusätzlich doppelsträngige RNA-Konstrukte für die Herstellung eines Arzneimittels zur Behandlung desIn a particularly preferred embodiment of the invention, in addition double-stranded RNA constructs for the manufacture of a medicament for the treatment of

Pankreaskarzinoms eingesetzt, die eine Sequenz ausgewählt aus einem der folgendenPancreatic carcinoma used a sequence selected from one of the following

Gene umfassen:Genes include:

CXCL5 (Nr. 1330, Tabelle 3, Affymetrix-Nr. 214974_x_at, Genname chemokine (C-X-C motif) ligand 5),CXCL5 (# 1330, Table 3, Affymetrix No. 214974_x_at, gene name chemokine (C-X-C motif) ligand 5),

FOXM 1 (Nr. 1272, Tabelle 3, Affymetrix-Nr. 202580_x_at, Genname forkhead box M1).FOXM 1 (# 1272, Table 3, Affymetrix No. 202580_x_at, common name forkhead box M1).

Die Erfindung betrifft weiterhin die Verwendung mindestens eines Polynukleotids, das eine Sequenz eines Gens umfasst, das in Tabelle 2 aufgeführt ist, zur Herstellung einer pharmazeutischen Zusammensetzung zur Behandlung des Pankreaskarzinoms beim Menschen, wobei das Polynukleotid den Krebszellen verabreicht wird, so dass das Gen in den Krebszellen exprimiert wird.The invention further relates to the use of at least one polynucleotide comprising a sequence of a gene listed in Table 2 for the preparation of a pharmaceutical composition for the treatment of human pancreatic carcinoma, wherein the polynucleotide is administered to the cancerous cells, such that the gene is inserted into the Cancer cells is expressed.

In einer alternativen Ausführungsform wird zusätzlich mindestens ein Polynukleotid, das eine Sequenz eines Gens umfasst, das in Tabelle 4 aufgeführt ist, zur Herstellung einer pharmazeutischen Zusammensetzung zur Behandlung des Pankreaskarzinoms beim Menschen verwendet, wobei das Polynukleotid den Krebszellen verabreicht wird, so dass das Gen in den Krebszellen exprimiert wird. Das Polynukleotid wird dazu in einen viralen Vektor eingebracht und den Patienten direkt in den Tumor oder intravenös appliziert. Das Polynukleotid, in Verbindung mit einem Promotor für die Transkription, kann auch als freie DNA dem Patienten in einem Komplex mit Lipiden oder ohne eine Lipid-Komplex dem Patienten intratumoral oder intra-venös zugeführt werden.In an alternative embodiment, in addition, at least one polynucleotide comprising a sequence of a gene listed in Table 4 is used to prepare a pharmaceutical composition for treating human pancreatic carcinoma, wherein the polynucleotide is administered to the cancer cells such that the gene is expressed in the cancer cells is expressed. The polynucleotide is introduced into a viral vector and the patient directly into the tumor or administered intravenously. The polynucleotide, in conjunction with a transcriptional promoter, may also be delivered as a free DNA to the patient in a complex with lipids or without a lipid complex intratumorally or intraveneously to the patient.

Durch nachfolgende Figuren und Ausführungsbeispiele werden die Erfindung und die der Erfindung zugrunde liegende wissenschaftliche Erkenntnis näher erläutert. Dabei zeigenThe invention and the scientific knowledge underlying the invention are explained in more detail by means of the following figures and exemplary embodiments. Show

Figur 1 :FIG. 1:

Fig. 1 zeigt photographische Aufnahmen einer manuellen Mikrodissektion von Pankreasgewebe. Dabei sind in der linken Spalte die Gewebeschnitte vor der Mikrodissektion und in der rechten Spalte nach der Mikrodissektion abgebildet. Die obere Zeile zeigt Gewebe aus duktalem Adenomakarzinom des Pankreas, die untere Zeile normales duktales Epithelgewebe.Fig. 1 shows photographs of a manual microdissection of pancreatic tissue. In the left column the tissue sections are shown before the microdissection and in the right column after the microdissection. The upper row shows tissue from ductal adenoma carcinoma of the pancreas, the lower row normal ductal epithelial tissue.

Figur 2:FIG. 2:

Fig. 2 zeigt photographische Aufnahmen präparierter Gewebeschnitte nach immunhistochemischer Analyse.Fig. 2 shows photographs of prepared tissue sections after immunohistochemical analysis.

Fig. 2 A, B zeigt eine Aufnahme eine CENPF-Proteinexpression in duktalenFigure 2 A, B is a photograph showing a CENPF protein expression in ductal

Adenokarzinomen des Pankreas (PDAC), während Fig. 2 C die nicht vorhandeneAdenocarcinomas of the pancreas (PDAC), while Fig. 2C the nonexistent

Expression des CENPF Proteins in normalem Pankreasgewebe zeigt.Expression of the CENPF protein in normal pancreatic tissue shows.

Fig. 2 D, E zeigt eine Aufnahme eine MCM7-Proteinexpression in duktalenFig. 2 D, E shows a picture of MCM7 protein expression in ductal

Adenokarzinomen des Pankreas (PDAC), während Fig. 2 F die nicht vorhandene Expression des MCM7 Proteins in normalem Pankreasgewebe zeigt.Pancreatic adenocarcinoma (PDAC), while Fig. 2F shows the non-existent expression of the MCM7 protein in normal pancreatic tissue.

Fig. 2 G, H zeigt eine Aufnahme eine MCM2-Proteinexpression in duktalenFig. 2G, H shows a picture of MCM2 protein expression in ductal

Adenokarzinomen des Pankreas (PDAC), während Fig. 2 I die nicht vorhandene Expression des MCM2 Proteins in normalem Pankreasgewebe zeigt.Pancreatic adenocarcinomas (PDAC), while Fig. 2 I shows the non-existent expression of the MCM2 protein in normal pancreatic tissue.

Figur 3:FIG. 3:

Fig. 3 zeigt die Validierung der differentiellen Expression mittels RT-PCR.Fig. 3 shows the validation of differential expression by RT-PCR.

Fig. 3 A: RT-PCR Analyse von 5 Genen in vier Tumor und korrespondierendemFig. 3A: RT-PCR analysis of 5 genes in four tumor and corresponding

Normalgewebe. Die entstandenen PCR Produkte wurden mittels einem Agarosegel separiert und mit Ethidiumbromidanfärbung sichtbar gemacht.Normal tissue. The resulting PCR products were separated by means of an agarose gel and visualized with ethidium bromide staining.

Fig. 3 B: Überblick über die RT-PCR Ergebnisse von Tumor- und Normalgewebe (oben), als auch Pankreaszellinien (unten). Weiss: nicht untersucht; Hellgrau: kein verwertbares PCR Signal; Mittelgrau: schwaches PCR Signal nachweisbar; Dunkelgrau: mittelstarkes PCR- Signal und Schwarz: Starkes PCR Signal nachweisbar. Die Housekeepinggene GAPDH und G6PD dienten als Vergleichskontrolle.Fig. 3 B: Overview of the RT-PCR results of tumor and normal tissue (top), as well as pancreatic cell lines (bottom). Weiss: not examined; Light gray: no usable PCR Signal; Middle gray: weak PCR signal detectable; Dark gray: medium PCR signal and black: strong PCR signal detectable. The housekeeping genes GAPDH and G6PD served as comparative controls.

Ausführungsbeispiel 1 : Identifizierung von Pankreaskarzinom-assoziierten GenenEmbodiment 1: Identification of pancreatic carcinoma-associated genes

Es wurde nach der Entnahme in flüssigem Stickstoff frisch eingefrorenes Gewebe aus duktalem Pankreaskarziom (N=14) und aus normalem Pankreasgewebe (N=11) verwendet. Das Normalgewebe entstammte aus Proben des Hauptganges, die keine sichtbaren dysplastischen Veränderungen enthielten.Freshly frozen tissue from ductal pancreatic carcinoma (N = 14) and normal pancreatic tissue (N = 11) was used after removal in liquid nitrogen. The normal tissue was derived from samples of the main gland that contained no visible dysplastic changes.

Von den Gewebestücken wurden dann wenige 10 μm dicke Schnitte geschnitten und sofort in 70% Ethanol fixiert. Diese Schnitte wurden mit Hämatoxylin und Eosin angefärbt und eingedeckt. Diese Probeschnitte dienten als Vorlage für die Mikrodissektion, da an ihnen die Areale eingezeichnet wurden, welche für die Mikrodissektion geeignet schienen. Von dem Gewebeblock wurden dann 5 μm dicke Serienschnitte angefertigt. Diese Schnitte wurden in RNase freiem 70% Ethanol fixiert und auch kurz mit Hämatoxylin und Eosin angefärbt. Tumor- und Normale Zellen wurden aus diesen Gewebeschnitten mit einer sterilen Kanüle aus den Schnitten herausgekratzt. Pro Gewebestück wurden ca. 10000 bis 11000 Zellen isoliert, wobei darauf geachtet wurde, dass ca. 95% des mikrodisseziierten Gewebes aus Tumor bzw. normalen Epitheizellen bestand (Fig. 1). Die Zellen wurden dann in eiskaltem GTC-Puffer (Guanidin-Thiocyanat-Puffer, Promege, Heidelberg) gesammelt. Aus den Zellen wurde dann die Poly-A+-RNA mittels eines Reaktionssystems (PolyAttract 1000, Promega, Heidelberg), gemäß der Anleitung, isoliert. Mit jeder Probe wurde dann dreimalig cDNA- Synthese mit anschliessender repetitiver /n-v/ϊ/O-Transkription durchgeführt (21 ). Zusammenfassend wird die cDNA-Synthese durch das Hybridisieren mit dem Affymetrix T7- oligo-dT Promoter-Primer in einer Konzentration von 0.1 mmol/l initiiert. Die Zweitstrang- Synthese geschieht durch internes Primen. Anschliessend wird eine /n-w'f/O-Transkription unter Verwendung des Ambion Megascript T7 Reaktionssystem (Ambion, Huntington, UK) nach Herstellerangaben durchgeführt. Die so hergestellte aRNA wird dazu verwendet, um erneut eine cDNA-Synthese zu initiieren, bei der die Zweitstrangsynthese durch Random- Hexamer Primer initiiert wird. Bei der dritten //7-wf/O-Transkription wird erneut so verfahren, allerdings werden dabei Biotin-markierte Nukleotide nach den Affymetrix-Herstellerangaben zugegeben. Die weitere Vorbereitung der Proben und die Hybridisierung verläuft nach den Angaben von Affymetrix.Of the tissue pieces were then cut a few 10 micron thick sections and immediately fixed in 70% ethanol. These sections were stained with hematoxylin and eosin and capped. These test sections served as a template for the microdissection, as they were drawn on the areas that seemed suitable for the microdissection. From the tissue block then 5 micron thick serial sections were made. These sections were fixed in RNase-free 70% ethanol and also stained briefly with hematoxylin and eosin. Tumor and normal cells were excised from these sections using a sterile cannula. About 10,000 to 11,000 cells were isolated per tissue piece, care being taken that about 95% of the microdissected tissue consisted of tumor or normal epithelial cells (FIG. 1). The cells were then collected in ice-cold GTC buffer (guanidine thiocyanate buffer, Promege, Heidelberg). From the cells, the poly A + RNA was then isolated by means of a reaction system (PolyAttract 1000, Promega, Heidelberg) according to the instructions. With each sample, three times cDNA synthesis followed by repetitive / nv / ϊ / O transcription was performed (21). In summary, the cDNA synthesis is initiated by hybridization with the Affymetrix T7 oligo dT promoter primer at a concentration of 0.1 mmol / l. The second strand synthesis is done by internal priming. Then a / nw 'f / is O transcription carried out using the Ambion MEGAscript T7 reaction system (Ambion, Huntington, UK) according to manufacturer's instructions. The aRNA thus prepared is used to re-initiate cDNA synthesis in which second-strand synthesis is initiated by random hexamer primers. The third // 7-wf / O transcription is repeated, but biotin-labeled nucleotides are added according to the Affymetrix manufacturer's instructions. The further preparation of the samples and the hybridization proceeds according to the data from Affymetrix.

Das Affymetrix U 133 Gene Chip Set besteht aus zwei GeneChips, auf welchen mehr als 44000 Probesets aufgetragen worden sind. Dadurch werden ca. 33.000 humane Gene und ca. 6000 humane ESTs repräsentiert. Nach der Hybridisierung werden die Signalintensitäten durch die Affymetrix MAS 5.0 Software ausgelesen und in der sogenannten Cel-Datei gespeichert. Diese 'wird dann zur weiteren Daten-Analyse verwendet. Zur Analyse werden die Dateien aller Experimente in das Programm dChip 1.3 (http://www.dchip.org) geladen, normalisiert und die Expressions-Werte, als auch die Werte für den Nachweis (Absolute CaIIs) werden mit dem PM/MM Modell bestimmt. Diese Werte werden dann in Excel und in SAM (http://www-stat.stanford.edu/ftibs/SAM/) eingelesen. Eine differentielle Expression wurde dann nachgewiesen, wenn folgende Kriterien erfüllt waren: Ein Quotient > 2 und ein q- Wert < 15% oder ein Presence call in wenigstens 60% eines Probentyps ohne einen Presence CaII in dem anderen Probentyp (Tabellen 1 bis A).The Affymetrix U 133 Gene Chip Set consists of two GeneChips on which more than 44000 sample sets have been applied. This represents approximately 33,000 human genes and approximately 6,000 human ESTs. After hybridization, the signal intensities through the Affymetrix MAS 5.0 software and stored in the so-called cel file. This' is then used for further data analysis. For analysis, the files of all experiments are loaded into the program dChip 1.3 (http://www.dchip.org), normalized and the expression values, as well as the values for the detection (Absolute CaIIs) with the PM / MM model certainly. These values are then read into Excel and SAM (http://www-stat.stanford.edu/ftibs/SAM/). Differential expression was detected when the following criteria were met: A quotient> 2 and a q value <15% or a presence call in at least 60% of one sample type without a presence CaII in the other sample type (Tables 1 to A).

Von den 616 differentiell exprimierten Gene wurden folgende zur Validierung mittels RT-PCR ausgewählt (Fig. 3):Of the 616 differentially expressed genes, the following were selected for validation by means of RT-PCR (FIG. 3):

PLAG1 (Gen-Nr. 159, Tabelle 1 ), PTTG1 (Gen-Nr. 1315, Tabelle 3), osf-2/POSTN (Gen-Nr. 1303, Tabelle 3), RAMP (Gen-Nr. 138, Tabelle 1), ECT2 (Gen-Nr. 506, Tabelle 1), CFLAR (Gen-Nr. 526, Tabelle 1), CCNB1 (Gen-Nr. 1287, Tabelle 3), CCNB2 (Gen-Nr. 203, Tabelle 1), UP/UPP1 (Gen-Nr. 176, Tabelle 1), MADD (Gen-Nr. 1269, Tabelle 3), LOXL2 (Gen-Nr. 184, Tabelle 1), HOXC6 (Gen-Nr. 158, Tabelle 1), EFNB2 (Gen-Nr. 186, Tabelle 1), IRAK1 (Gen-Nr. 370, Tabelle 1 ) und CENPF (Gen-Nr. 108, Tabelle 1). Die Housekeepinggene GAPDH und G6PD dienten als Vergleichskontrolle.PLAG1 (gene No. 159, Table 1), PTTG1 (gene No. 1315, Table 3), osf-2 / POSTN (gene No. 1303, Table 3), RAMP (gene No. 138, Table 1 ), ECT2 (Gene No. 506, Table 1), CFLAR (Gene No. 526, Table 1), CCNB1 (Gen No. 1287, Table 3), CCNB2 (Gene No. 203, Table 1), UP / UPP1 (Gene No. 176, Table 1), MADD (Gen No. 1269, Table 3), LOXL2 (Gene No. 184, Table 1), HOXC6 (Gene No. 158, Table 1), EFNB2 (Gen. No. 186, Table 1), IRAK1 (Gen. No. 370, Table 1) and CENPF (Gen. No. 108, Table 1). The housekeeping genes GAPDH and G6PD served as comparative controls.

Für die RT-PCR Analyse wurden 9 Proben von Normalgewebe und 13 von PDAC-(duktales adenokarzinom des Pankreas) Gewebe auf die Expression der ausgewählten Gene untersucht. Dazu wurde die RNA aus normalem pankreatischen Gewebe und von pankreatischem Tumorgewebe mit Hilfe des „Micro to Mini Total RNA Purification Kit" (Invitrogen) nach Herstellerangaben isoliert. Nach der reversen Transkription mit random Hexamer-Primem und der „Superscript" Reversen Transkriptase (Invitrogen) folgte eine PCR Amplifikation (580C Annealing Temperatur, 27 Zyklen) unter Standardbedingungen. Die Primer für die PCR-Amplifikation wurden von MWG-Biotech, Ebersberg, synthetisiert. Es wurden folgende Primer eingesetzt:For RT-PCR analysis, 9 samples of normal tissue and 13 of PDAC (ductal adenocarcinoma of the pancreas) tissue were examined for expression of the selected genes. RNA was isolated from normal pancreatic tissue and from pancreatic tumor tissue using the Micro to Mini Total RNA Purification Kit (Invitrogen) according to the manufacturer's instructions After reverse transcription with random hexamer primers and the "Superscript" reverse transcriptase (Invitrogen) followed by a PCR amplification (58 0 C annealing temperature, 27 cycles) under standard conditions. The primers for PCR amplification were synthesized by MWG-Biotech, Ebersberg. The following primers were used:

GAPDH δ'-CCAGCCGAGCCACATCGCTC-S' (SEQ-ID 1) δ'-ATGAGCCCCAGCCTTCTCCAT-S' (SEQ-ID 2)GAPDH δ'-CCAGCCGAGCCACATCGCTC-S '(SEQ ID 1) δ'-ATGAGCCCCAGCCTTCTCCAT-S' (SEQ ID 2)

G6PD δ'-ACGTGATGCAGAACCACCTACTG-S' (SEQ-ID3)G6PD δ'-ACGTGATGCAGAACCACCTACTG-S '(SEQ ID3)

5ΑCGACGGCTGCAAAAGTGGCG-3' (SEQ-ID4)5ΑCGACGGCTGCAAAAGTGGCG-3 '(SEQ ID 4)

CCNB1 ö'-GATATCTATCAGTATCTCAGGCAGCTG-S' (SEQ-ID 5) δ'-ATACTTGGAAGCCAAGAGCAGAGC-S' (SEQ-ID 6)CCNB1 ö'-GATATCTATCAGTATCTCAGGCAGCTG-S '(SEQ ID 5) δ'-ATACTTGGAAGCCAAGAGCAGAGC-S' (SEQ ID 6)

CCNB2CCNB2

Ö'-GATATCTATCAGTATCTCAGGCAGCTG-S' (SEQ-ID 7) δ'-ATACTTGGAAGCCAAGAGCAGAGC-S' (SEQ-ID 8)Ö'-GATATCTATCAGTATCTCAGGCAGCTG-S '(SEQ ID 7) δ'-ATACTTGGAAGCCAAGAGCAGAGC-S' (SEQ ID 8)

CENPFCENPF

5'-GCGGCAGAAAAGAAACAGAC-3I (SEQ-ID 9) δ'-TCTTCTGTGTCGATGCCAAG-S' (SEQ-ID 10)5'-GCGGCAGAAAAGAAACAGAC I-3 (SEQ ID 9) δ'-TCTTCTGTGTCGATGCCAAG-S '(SEQ ID 10)

CFLAR δ'-ATGGACAGAAAAGCTGTGGAG-S' (SEQ-ID 11) δ'-CTTCAGGTCTATTCTGTGGATG-S' (SEQ-ID 12)CFLAR δ'-ATGGACAGAAAAGCTGTGGAG-S '(SEQ ID 11) δ'-CTTCAGGTCTATTCTGTGGATG-S' (SEQ ID 12)

EFNB2 δ'-CTGCTGGATCAACCAGGAAT-S' (SEQ-ID 13)EFNB2 δ'-CTGCTGGATCAACCAGGAAT-S '(SEQ ID 13)

5'-CTGTTGCCGTCTGTGCTAGA-3' (SEQ-ID 14)5'-CTGTTGCCGTCTGTGCTAGA-3 '(SEQ ID 14)

H0XC6 δ'-ACAGACCTCAATCGCTCAGG-S' (SEQ-ID 15) δ'-GGTACCGCGAGTAGATCTGG-S' (SEQ-ID 16)H0XC6 δ'-ACAGACCTCAATCGCTCAGG-S '(SEQ ID 15) δ'-GGTACCGCGAGTAGATCTGG-S' (SEQ ID 16)

L0XL2 δ'-CAGACCACCTACCTGGAGGA-S' (SEQ-ID 17) δ'-GTTGTGGATCTGGGAGGAGA-S' (SEQ-ID 18)L0XL2 δ'-CAGACCACCTACCTGGAGGA-S '(SEQ ID 17) δ'-GTTGTGGATCTGGGAGGAGA-S' (SEQ ID 18)

MADD δ'-TGTGCAGGACCTGAAGACTG-S' (SEQ-ID 19)MADD δ'-TGTGCAGGACCTGAAGACTG-S '(SEQ ID 19)

5'-ACAAAGACGCCTCGAACTGT-3' (SEQ-ID 20) osf-2 δ'-TGCATTATTCACAGGTGCCAG-S' (SEQ-ID 21) δ'-ACTCTCCAGTGTTCTGAGTC-S' (SEQ-ID 22)5'-ACAAAGACGCCTCGAACTGT-3 '(SEQ ID 20) osf-2 δ'-TGCATTATTCACAGGTGCCAG-S' (SEQ ID 21) δ'-ACTCTCCAGTGTTCTGAGTC-S '(SEQ ID 22)

PTTG1 ö'-AAGGAAAATGGAGAACCAGGC-S' (SEQ-ID 23) δ'-GCTTGGCTGTTTTTGTTTGAGG-S' (SEQ-ID 24)PTTG1 ö'-AAGGAAAATGGAGAACCAGGC-S '(SEQ ID 23) δ'-GCTTGGCTGTTTTTGTTTGAGG-S' (SEQ ID 24)

RAMPRAMP

5ΑCAGCAGCAGGTGATCAAACAGC-3' (SEQ-ID 25)5ΑCAGCAGCAGGTGATCAAACAGC-3 '(SEQ ID 25)

5'-GAAGGAGCAGAGTCCTTTTGAATTCTG-S' (SEQ-ID 26)5'-GAAGGAGCAGAGTCCTTTTGAATTCTG-S '(SEQ ID 26)

PLAG1 δ'-TTTCCTTGCCAACTGTGTGAC-S' (SEQ-ID 27) δ'-CTTTGTTAGGGTCGTGTGTATG-S' (SEQ-ID 28)PLAG1 δ'-TTTCCTTGCCAACTGTGTGAC-S '(SEQ ID 27) δ'-CTTTGTTAGGGTCGTGTGTATG-S' (SEQ ID 28)

ECT2 δ'-ACTAGCTTGGCAGACTCTTC-S' (SEQ-ID 29) δ'-ATCCTGAAAGTCCGTGACTAC-S' (SEQ-ID 30)ECT2 δ'-ACTAGCTTGGCAGACTCTTC-S '(SEQ ID 29) δ'-ATCCTGAAAGTCCGTGACTAC-S' (SEQ ID 30)

UP δ'-CGGAAAACGGACCTTAACAA-S' (SEQ-ID 31) δ'-GATACGCCTGCTTGTCCTTC-S' (SEQ-ID 32) Die PCR-Produkte wurden mittels Agarosegelelektrophorese separiert und mit Ethidiumbromid angefärbt.UP δ'-CGGAAAACGGACCTTAACAA-S '(SEQ ID 31) δ'-GATACGCCTGCTTGTCCTTC-S' (SEQ ID 32) The PCR products were separated by agarose gel electrophoresis and stained with ethidium bromide.

Die Gesamt-RNA aus pankreatischen Zelllinien wurde mit dem RNAeasy RNA isolation kit (Qiagen, Hilden) isoliert und wie oben in cDNA transkribiert. Zur quantitativen PCR-Detektion wurde ein ABI Sequence Detection System 7700 und der Sybr Green Master Mix (ABI, Weiterstadt) nach Herstellerangaben eingesetzt.The total RNA from pancreatic cell lines was isolated with the RNAeasy RNA isolation kit (Qiagen, Hilden) and transcribed into cDNA as above. For quantitative PCR detection, an ABI Sequence Detection System 7700 and the Sybr Green Master Mix (ABI, Weiterstadt) were used according to the manufacturer's instructions.

Ausführungsbeispiel 2: Immunhistochemie zum Nachweis der MCM2, der MCM7 oder der CENPF Proteinexpression in Pankreastumoren zur Diagnose:Exemplary Embodiment 2 Immunohistochemistry for Detecting MCM2, MCM7 or CENPF Protein Expression in Pancreatic Tumors for Diagnosis

Figur 2 zeigt die Ergebnisse einer immunohistochemischen Analyse der MCM2 (Gen-Nr.FIG. 2 shows the results of an immunohistochemical analysis of MCM2 (gene no.

107, Tabelle 1 ), MCM7 (Gen-Nr. 395, Tabelle 1) und der CENPF-Proteinexpression (Gen-Nr.107, Table 1), MCM7 (Gene No. 395, Table 1), and CENPF protein expression (Gen.

108, Tabelle 1 ) in Pankreasgewebeschnitten. Zur Herstellung dieser Schnitte wird Pankreasgewebe in einem operativen Eingriff entnommen und zur Fixierung in gepuffertem 4% Formalin eingelegt. Nach der Fixierung wird das Gewebe nach einer gängigen Methode in Paraffin eingebettet (22). Von diesem in Paraffin eingebetteten Gewebe werden Schnitte mit einer Dicke von 4 μm mit einem Mikrotom (Leica, Nussloch) angefertigt und auf speziell vorbereitete Objektträger (Superfrost; Menzel Gläser, Braunschweig) aufgezogen. Danach werden die Schnitte durch eine Inkubation in XyIoI von dem Paraffin befreit. Danach werden die Schnitte durch die Inkubation in einer absteigenden Alkoholreihe (100% Ethanol bis 0% Ethanol) rehydriert.108, Table 1) in pancreatic tissue sections. For the preparation of these sections, pancreatic tissue is removed in an operative procedure and inserted for fixation in buffered 4% formalin. After fixation, the tissue is embedded in paraffin by a common method (22). Sections 4 μm thick are prepared from this paraffin-embedded tissue with a microtome (Leica, Nussloch) and mounted on specially prepared slides (Superfrost, Menzel Gläser, Braunschweig). The sections are then freed from the paraffin by incubation in xylene. The sections are then rehydrated by incubation in a descending alcohol series (100% ethanol to 0% ethanol).

Danach werden die Schnitte für 2 min in einem Schnellkochtopf gekocht während sie in einem Tris-EDTA-Citrat Puffer verbleiben und anschließend mit destilliertem Wasser gewaschen. Danach werden die Schnitte mit einer Lösung eines MCM2, MCM7 oder CENPF spezifischen primären Antikörper versetzt. Dieser Antikörper, wie z. B. ein mit humanem CENPF (Klon D8; Abcam Ltd., Cambridge, UK), humanem MCM2 (Klon CRCT2; Novocastra, Newcastle, UK), oder humanem MCM7 (Klon CRCT7; Novocastra), werden dazu in definierten Konzentration in einer Lösung aus PBS welcher 2% Pferdeserum (erhältlich von Vector Laboratories, Alexis, Grünberg) enthält gelöst. Nach einer Inkubation der Schnitte mit der Antikörperlösung für 45 min bei Raumtemperatur, werden die Schnitte 2 mal mit PBS für jeweils 5 min gewaschen.The slices are then cooked for 2 minutes in a pressure cooker while remaining in a Tris-EDTA citrate buffer and then washed with distilled water. The sections are then spiked with a solution of MCM2, MCM7 or CENPF specific primary antibody. This antibody, such as. For example, one with human CENPF (clone D8, Abcam Ltd., Cambridge, UK), human MCM2 (clone CRCT2, Novocastra, Newcastle, UK), or human MCM7 (clone CRCT7, novocastra) are added thereto in defined concentration in a solution from PBS containing 2% horse serum (available from Vector Laboratories, Alexis, Grünberg). After incubating the sections with the antibody solution for 45 min at room temperature, the sections are washed twice with PBS for 5 min each.

Die Bindung dieser Antikörper an die jeweiliegen Zielmoleküle wird mit der folgenden Reaktion nachgewiesen:The binding of these antibodies to the respective target molecules is detected by the following reaction:

Die Schnitte werden mit einem biotinylierten anti-Ziegen-lgG Antikörper (erhältlich als fertiges Reagenziensystem bei Vector Laboratories) in einer Konzentration von 5μg/ml inkubiert. Danach werden die Schnitte mit dem Reagenziensystem Avidin-Biotin Peroxidase (ABC ELITE, Vector Laboratories) inkubiert. Dabei wird nach der Beschreibung in denThe sections are incubated with a biotinylated anti-goat IgG antibody (available as a finished reagent system from Vector Laboratories) at a concentration of 5 μg / ml. Thereafter, the sections with the reagent system avidin-biotin peroxidase (ABC ELITE, Vector Laboratories). It is according to the description in the

Reagenzienssystemen verfahren. Zum Schluss werden die Schnitte mit einer Lösung vonMove reagent systems. Finally, the cuts are made with a solution of

Diaminobenzidin (Sigma, Taufkirchen) in 0,05 mol/l Tris-HCI pH7,0 bei Raumtemperatur inkubiert. Danach werden die Schnitte mit einem Standardverfahren mit Hematoxylin gefärbt und eingedeckt.Diaminobenzidine (Sigma, Taufkirchen) in 0.05 mol / l Tris-HCl pH7.0 at room temperature. The sections are then stained and covered with a standard hematoxylin procedure.

Die Färbung wird dann mikroskopisch beurteilt. Dabei steht eine positive Färbung für eineThe staining is then assessed microscopically. It stands for a positive coloring for a

Proteinexpression.Protein expression.

Ein Tumor äußert sich dabei in der Anfärbung der Zellen für eines der drei Proteine, während nichtmalignes Gewebe keine oder nur vernachlässigbare geringe Anfärbung zeigt.A tumor manifests itself in the staining of the cells for one of the three proteins, while non-malignant tissue shows little or no negligible staining.

Photographische Aufnahmen derart präparierter Gewebeschnitte werden in Fig. 2 dargestellt:Photographs of such prepared tissue sections are shown in FIG. 2:

Ausführungsbeispiel 3: Nachweis CENPF-mRNA-ExpressionExemplary Embodiment 3 Detection of CENPF mRNA Expression

Die Analyse der CENPF-mRNA-Expression (Gen-Nr. 108, Tabelle 1) erfolgt in isolierter RNA aus Gewebestücken des Pankreas zum Nachweis eines Tumors.Analysis of CENPF mRNA expression (gene number 108, Table 1) is performed in isolated RNA from pancreatic tissue pieces to detect a tumor.

Dazu wird die RNA mit Standardverfahren und Reagenziensystemen aus Gewebestücken des Pankreas isoliert (RNeasy der Fa. Qiagen, Hilden, Deutschland). Dabei wird nach dem Protokoll des Reagenziensystems verfahren. Danach wird die RNA mit dem Enzym Reverse Transkriptase in cDNA umgeschrieben. Die dazu notwendige Transkriptionsinitiierung wird hierbei von „Random Hexameren" (Oligonucleotide bestehend aus je 6 Nukleotiden mit variabler Sequenz) übernommen (Invitrogen, Karlsruhe). Es werden zu dem Ansatz 100 pg der „Random Hexamere" gegeben. Die Synthese wird ansonsten gemäss der Beschreibung des Herstellers durchgeführt. Nach dieser Reaktion wird die cDNA in eine quantitative PCR eingesetzt. Dazu wird 1 μl der cDNA-Lösung verwendet. Die cDNA wird zu einem Ansatz von 24 μl aus einem Mastermix bestehend aus Puffer, Taq-DNA-Polymerase (Sybr Green PCR Mastermix der Fa. Applied Biosystems, Weiterstadt) und den genspezifischen Primern 5'~ GTCAGCGACAAAATGCAGAA-S' (SEQ-ID 33) und δ'-ACTCCTGGTCCAGTGTTTGG-S' (SEQ-ID 34) gegeben. Dabei liegen in dem Mix die Primer in einer Konzentration von 300 nmol/l vor. Zur Herstellung dieses Mixes werden für jede Bestimmung 12,5 μl des Sybr Green PCR Mastermix jeweils 1 μl der Primer aus einer Lösung mit einer Konzentration von 3 μmol/l und 9,5 μl destilliertes Wasser gemischt. Danach wird eine PCR unter Standardbedingungen im ABI Prism Sequence Detection System durchgeführt und die Werte für die enthaltene RNA Menge werden nach der Durchführung der PCR errechnet. Dabei wird die Fluoreszenz des in die entstehenden DNA Fragmente eingelagerten Sybr Greens während der PCR gemessen und die RNA-Menge analog zum TaqMan Verfahren errechnet.For this purpose, the RNA is isolated from tissue pieces of the pancreas using standard methods and reagent systems (RNeasy from Qiagen, Hilden, Germany). In doing so, proceed according to the protocol of the reagent system. Thereafter, the RNA is transcribed into cDNA with the enzyme reverse transcriptase. The transcription initiation necessary for this is taken over by "random hexamers" (oligonucleotides each consisting of 6 nucleotides with variable sequence) (Invitrogen, Karlsruhe), to which 100 pg of the "random hexamers" are added. The synthesis is otherwise carried out according to the manufacturer's description. After this reaction, the cDNA is used in a quantitative PCR. For this, 1 μl of the cDNA solution is used. The cDNA is added to a batch of 24 μl from a master mix consisting of buffer, Taq DNA polymerase (Sybr Green PCR master mix from Applied Biosystems, Weiterstadt) and the gene-specific primers 5 '~ GTCAGCGACAAAATGCAGAA-S' (SEQ ID 33 ) and δ'-ACTCCTGGTCCAGTGTTTGG-S '(SEQ ID 34). In this case, the primers are present in a concentration of 300 nmol / l in the mix. To prepare this mix, for each determination, mix 12.5 μl of the Sybr Green PCR Master Mix with 1 μl each of the primers from a solution with a concentration of 3 μmol / l and 9.5 μl distilled water. Thereafter, a PCR is performed under standard conditions in the ABI Prism Sequence Detection System and the values for the amount of RNA contained are calculated after carrying out the PCR. there the fluorescence of the embedded in the DNA fragments Sybr Green is measured during the PCR and the amount of RNA calculated analogous to the TaqMan method.

Analog Ausführungsbeispiel 3 werden auch das Gen FOXM 1 (Gen-Nr. 1272, Tabelle 3) mit den Primern δ'-ATCCAACATCCAGTGGCTTC-S' (SEQ-ID 35) und 5'- TCGAAGGCTCCTCAACCTTA3' (SEQ-ID 36), das Gen AATF (Gen-Nr. 391 , Tabelle 1) mit den Primern 5'-CTTGGACACGGACAAAAGGT-3' (SEQ-ID 37) und 5'- CACACTCCTGTTCCTCAGCA-3' (SEQ-ID 38) und das Gen BCAR3 (Gen-Nr. 73, Tabelle 1) mit den Primern 5'-AAGTTCCTTCCCCCTGAGÄA-3' (SEQ-ID 39) und 5'- CCTGCAGTCCATGCTCAGTA-S' (SEQ-ID 40) bestimmt.Analogously to embodiment 3, the gene FOXM 1 (gene No. 1272, Table 3) with the primers δ'-ATCCAACATCCAGTGGCTTC-S '(SEQ ID 35) and 5'-TCGAAGGCTCCTCAACCTTA3' (SEQ ID 36), the gene AATF (Gen. No. 391, Table 1) with the primers 5'-CTTGGACACGGACAAAAGGT-3 '(SEQ ID 37) and 5'-CACACTCCTGTTCCTCAGCA-3' (SEQ ID 38) and the gene BCAR3 (Gen.-No. 73, Table 1) with the primers 5'-AAGTTCCTTCCCCCTGAGÄA-3 '(SEQ ID 39) and 5'-CCTGCAGTCCATGCTCAGTA-S' (SEQ ID 40).

Ausführungsbeispief 4: Bestimmung der CKLF Menge im Serum zur Diagnose eines Pankreas-KarzinomsEMBODIMENT 4: Determination of the amount of CKLF in the serum for the diagnosis of a pancreatic carcinoma

Die Bestimmung der CKLF-Menge (Gen-Nr. 538, Tabelle 1) im Serum erfolgt nach Standardverfahren zum Nachweis von Proteinen im Serum. Das Serum wird von Patienten aus dem venösen Blut gewonnen und nach der Gerinnung verwendet. In einen mit Antikörpern gegen CKLF beschichtete Napf wird dann das Serum eingefüllt und 60 min gewartet, damit das Protein an die Antikörper binden kann. Danach wird der Napf mit PBS für dreimal gewaschen um überflüssiges Protein abzuwaschen. Diese Bindung wird dann mit einem zweiten gegen CKLF gerichteten Antikörper nachgewiesen. Dazu wird dieser Antikörper zu dem Napf gegeben und 60 min bei RT gewartet. Erneut wird der Napf dreimal mit PBS gewaschen. Die Bindung des zweiten Antikörpers wird durch die Zugabe eines dritten Antikörpers nachgewiesen. Dieser dritte Antikörper wird in den Napf gefüllt und es wird erneut 60 min abgewartet um die Bindung zu ermöglichen. Danach wird Peroxidase verbunden. Durch die Zugabe eines Substrates wird die gebundene Menge bestimmt.The determination of the amount of CKLF (Gene No. 538, Table 1) in serum is carried out according to standard methods for the detection of proteins in serum. Serum is collected from venous blood patients and used after coagulation. The serum is then loaded into a cup coated with antibodies against CKLF and waited 60 minutes for the protein to bind to the antibodies. Thereafter, the well is washed with PBS for three times to wash off excess protein. This binding is then detected with a second antibody directed against CKLF. For this purpose, this antibody is added to the well and waited for 60 min at RT. Again, the dish is washed three times with PBS. The binding of the second antibody is detected by the addition of a third antibody. This third antibody is filled into the well and it is again waited 60 min to allow the binding. Thereafter, peroxidase is linked. By adding a substrate, the bound amount is determined.

Analog Ausführungsbeispiel 4 wird auch die KLK10 (Gen-Nr. 1279, Tabelle 3) im Serum bestimmt. Dazu wird in einen mit Antikörpern gegen KLK10 beschichteten Napf das Serum eingefüllt und 60 min gewartet, damit das Protein an den Antikörper binden kann. Danach wird der Napf dreimal mit PBS gewaschen, um überschüssiges Protein abzuwaschen. Diese Bindung wird dann mit einem zweiten gegen KLK10 gerichteten Antikörper nachgewiesen Dazu wird dieser Antikörper zu dem Napf gegeben und 60 min bei RT gewartet. Erneut wird der Napf dreimal mit PBS gewaschen. Die Bindung des zweiten Antikörpers wird durch die Zugabe eines dritten Antikörpers nachgewiesen. Dieser dritte Antikörper wird in den Napf gefüllt und es wird erneut 60 min abgewartet um die Bindung zu ermöglichen. Danach wird Peroxidase verbunden. Durch die Zugabe eines Substrates wird die gebundene Menge bestimmt.Analogously to embodiment 4, the KLK10 (gene No. 1279, Table 3) is also determined in the serum. For this purpose, the serum is filled in a cup coated with antibodies against KLK10 and waited for 60 min, so that the protein can bind to the antibody. Thereafter, the well is washed three times with PBS to wash off excess protein. This binding is then detected with a second antibody directed against KLK10. This antibody is added to the well and maintained at RT for 60 min. Again, the dish is washed three times with PBS. The binding of the second antibody is detected by the addition of a third antibody. This third antibody is filled into the well and it is again waited 60 min to allow the binding. After that will Linked to peroxidase. By adding a substrate, the bound amount is determined.

Bei einer Untersuchung von acht Patienten mit Pankreaskarziom wurde im Mittel eineIn an investigation of eight patients with pancreatic carcinoma on average one

Menge von 1,62 μg/L nachgewiesen, während bei Patienten mit einer gutartigenAmount of 1.62 μg / L, while in patients with a benign

Pankreaserkrankung im Mittel nur 0,81 μg/L nachgewiesen werden konnten.Pancreatic disease on average only 0.81 μg / L could be detected.

Ausführungsbeispiel 5: Therapie des Pankreaskarzioms mittels siRNA-MolekülenExemplary Embodiment 5 Therapy of Pancreatic Carcinoma by means of siRNA Molecules

Zu der Therapie des Pankreaskarzioms werden Patienten die siRNA Moleküle 5'- CCUUCUCUAGUGUCAAAGU-S" (SEQ-ID 41), δ'-CGAUAAGACCAAACUGGCU-S' (SEQ-ID 42), δ'-UGGACAAAGAGAGAUUACU-S' (SEQ-ID 43) und 5'-For the treatment of pancreatic carcinoma patients are the siRNA molecules 5'-CCUUCUCUAGUGUCAAAGU-S "(SEQ ID 41), δ'-CGAUAAGACCAAACUGGCU-S '(SEQ ID 42), δ'-UGGACAAAGAGAGAUUACU-S' (SEQ ID 43 ) and 5'-

UGGAUGACCUUGGGAGCAG-3' (SEQ-ID 44) bzw. 5'-CAUGCCGGUGAAUCACCAG-3' (SEQ-ID 45), δ'-GCACGUACUGAGCAUGGAC-S' (SEQ-ID 46), 5'-UGGAUGACCUUGGGAGCAG-3 '(SEQ ID 44) or 5'-CAUGCCGGUGAAUCACCAG-3' (SEQ ID 45), δ'-GCACGUACUGAGCAUGGAC-S '(SEQ ID 46), 5'-

CCAUUUGGCAACAGCGCGA-S1 (SEQ-ID 47) und 5'-GAGCUACCUGCCGAUUGGC-3' (SEQ-ID 48) intravenös zugeführt. Die siRNAs der SEQ-Ids 41 bis 44 wirken hierbei gegen die Expression des Proteins AATF (Gen-Nr. 391 , Tabelle 1) und die der SEQ-Ids 45 bis 48 gegen die Expression des Proteins BCAR3 (Gen-Nr. 73, Tabelle 1 ). Dieses Protein spielt bei der Signaltransduktion eine wichtige Rolle und ist im Pankreaskarzinom überexprimiert. Die siRNA wird chemisch synthetisiert. Dabei werden zunächst die komplementären Stränge des Moleküls einzeln gemäß Standardverfahren für die Synthese von Nukleinsäuren synthetisiert und dann in einem zweiten Schritt hybridisiert, damit so doppelsträngige siRNA-Moleküle entstehen.CCAUUUGGCAACAGCGCGA-S 1 (SEQ ID 47) and 5'-GAGCUACCUGCCGAUUGGC-3 '(SEQ ID 48) were delivered intravenously. The siRNAs of SEQ ID Nos. 41 to 44 counteract the expression of the protein AATF (gene No. 391, Table 1) and those of SEQ ID Nos. 45 to 48 against the expression of the protein BCAR3 (gene No. 73, Table 1 ). This protein plays an important role in signal transduction and is overexpressed in pancreatic carcinoma. The siRNA is chemically synthesized. Initially, the complementary strands of the molecule are individually synthesized according to standard procedures for the synthesis of nucleic acids and then hybridized in a second step, so as to form double-stranded siRNA molecules.

Ausführungsbeispiel 6: Therapie des Pankreaskarzioms mittels Antisense- KonstruktenEmbodiment 6 Therapy of Pancreatic Carcinoma by Antisense Constructs

Als Antisense-Konstrukte für das Gen BCAR3 (Gen-Nr. 73, Tabelle 1 ) wird die Sequenz 5'- CATGCCGGTGAATCACCAG-3' (SEQ-ID 49), für das Gen AATF (Gen-Nr. 391 , Tabelle 1) 5'-CCTTCTCTAGTGTCAAAGT-3' (SEQ-ID 50) eingesetzt. Ausführungsbeispiel 7: knock-down Experimente mittels enzymatisch hergestellter siRNAAs antisense constructs for the gene BCAR3 (Gene No. 73, Table 1), the sequence 5'-CATGCCGGTGAATCACCAG-3 '(SEQ ID 49), for the gene AATF (Gene No. 391, Table 1) 5 '-CCTTCTCTAGTGTCAAAGT-3' (SEQ ID 50). Exemplary embodiment 7: knock-down experiments by means of enzymatically produced siRNA

Von den Genen Nr. 232 (Tabelle 1, Gensymbol APLP2, Affymetrix-Probennummer 214875_x_at), Nr. 1330 (Tabelle 3, Gensymbol CXCL5, Affymetrix-Probennummer 214974_x_at), Nr. 506 (Tabelle 1 , Gensymbol ECT2, Affymetrix-Probennummer 219787_s_at), Nr. 311 (Tabelle 1, Gensymbol FLJ11323, Affymetrix-Probennummer 218951_s_at), Nr. 1272 (Tabelle 3, Gensymbol FOXM1 , Affymetrix-Probennummer 202580_x_at), und Nr. 103 (Tabelle 1 , Gensymbol TTK, Affymetrix-Probennummer 204822_at) werden cDNA-Klone hergestellt, wobei an beide Enden der Gene eine T7 Promotor-Sequenz mittels PCR angefügt wird. Mittels in vitro Transkription unter Verwendung des Megascript T7-Kits (Ambion) wird die cDNA in RNA überführt. Dazu werden nach Herstellerangaben 1 μg der cDNA mit 2 μl Transkriptionspuffer, jeweils 2 μl Ribonukleotiden (ATP, GTP, CTP und UTP) und 1 μl Enzymmix versetzt und bei 37°C über Nacht inkubiert. Die RNA wird unter Verwendung des RNeasy Mini Kits (Qiagen) gereinigt und die erhaltene Menge an RNA wird durch Messung der optischen Dichte bestimmt. 8 μg der RNA werden dann mit dem Enzym Dicer und Reaktionspuffer versetzt und über Nacht bei 37°C inkubiert. Nach Beendigung der Inkubation wird die Reaktion mittels G25 Säulen und Ultrafiltrationssäulen (Microcon YM-100, Firma Millipore) gereinigt. Die erhaltene siRNA befindet sich nach der Zentrifugati on durch den Ultrafiltrationsfilter im Durchlauf. Die erhaltene siRNA wird durch Vakuumzentrifugation konzentriert und die Menge wird mittels einer Gelelektrophorese im Vergleich zu einem bekannten Mengenstandard bestimmt.From Genes # 232 (Table 1, Gene Symbol APLP2, Affymetrix Sample Number 214875_x_at), # 1330 (Table 3, Gene Symbol CXCL5, Affymetrix Sample Number 214974_x_at), # 506 (Table 1, Gene Symbol ECT2, Affymetrix Sample Number 219787_s_at) , No. 311 (Table 1, Gene Symbol FLJ11323, Affymetrix Sample Number 218951_s_at), No. 1272 (Table 3, Gene Symbol FOXM1, Affymetrix Sample Number 202580_x_at), and No. 103 (Table 1, Gene Symbol TTK, Affymetrix Sample Number 204822_at) cDNA clones prepared, wherein at both ends of the genes a T7 promoter sequence is added by PCR. By means of in vitro transcription using the Megascript T7 kit (Ambion), the cDNA is converted into RNA. For this purpose, according to the manufacturer's instructions, 1 μg of the cDNA is mixed with 2 μl of transcription buffer, in each case 2 μl of ribonucleotides (ATP, GTP, CTP and UTP) and 1 μl of enzyme mix, and incubated at 37 ° C. overnight. The RNA is purified using the RNeasy Mini Kit (Qiagen) and the amount of RNA obtained is determined by measuring the optical density. 8 .mu.g of the RNA are then mixed with the enzyme Dicer and reaction buffer and incubated overnight at 37.degree. After completion of the incubation, the reaction is purified using G25 columns and ultrafiltration columns (Microcon YM-100, Millipore). The siRNA obtained is after centrifugation through the ultrafiltration filter in the flow. The resulting siRNA is concentrated by vacuum centrifugation and the amount is determined by gel electrophoresis as compared to a known quantity standard.

Die siRNA wird auf eine Konzentration von 10 ng/μl eingestellt und dann zur Transfektion der Pankreaskarzinomzelllinien Panc89 und MiaPaCa-2 verwendet. Dazu werden ca. 5.000 Zellen in eine Vertiefung einer 96-Loch-Platte ausgesät und einen Tag später transfiziert. Für die Panc89-Zellen werden jeweils 30 ng siRNA mit 0,15 μl Oligofectamine Reagenz gemäß Herstellerprotokoll (Invitrogen) versetzt und auf die Zellen gegeben. Für die MiaPaCa-2- Zellen werden je 50 ng siRNA mit 0,3 μl Oligofectamine Reagenz gemäß Herstellerprotokoll (Invitrogen) versetzt und auf die Zellen gegeben. Nach 72 Stunden wird das Ausmaß an Apoptose gemessen. Dazu werden die Zellen mit FITC markiertem Annexin V und Draqδ gefärbt und die Färbeintensität wird gemessen. Aus dem Verhältnis der Annexin V Färbung zur Draqδ Färbung ergibt sich der sog. Annexin-Index, der ein Maß für die Apoptoseinduktion darstellt. In Tabelle 5 sind die für die Gene Nr. 232, 1330, 506, 311 , 1272 und 103 ermittelten Annexin-Indizes im Vergleich zum Annexin-Index einer Positiv-Kontrolle und einer Negativ- Kontrolle aufgeführt (jeweils als Log2 des Mittelwerts aus sechs Experimenten).The siRNA is adjusted to a concentration of 10 ng / μl and then used to transfect the pancreatic carcinoma cell lines Panc89 and MiaPaCa-2. For this purpose, about 5,000 cells are seeded in a well of a 96-well plate and transfected one day later. For the Panc89 cells, in each case 30 ng siRNA are mixed with 0.15 μl Oligofectamine reagent according to the manufacturer's protocol (Invitrogen) and applied to the cells. For the MiaPaCa-2 cells, 50 μg of siRNA are mixed with 0.3 μl of Oligofectamine reagent according to the manufacturer's protocol (Invitrogen) and applied to the cells. After 72 hours, the extent of apoptosis is measured. For this purpose, the cells are stained with FITC-labeled annexin V and Draqδ and the staining intensity is measured. From the ratio of annexin V staining to Draqδ staining, the so-called annexin index results, which represents a measure for the apoptosis induction. Table 5 lists the annexin indices determined for genes Nos. 232, 1330, 506, 311, 1272 and 103 in comparison to the annexin index of a positive control and a negative control (each as Log2 of the mean of six experiments ).

Die durch den Einsatz der siRNA verminderte Expression der im Pankreaskarziom spezifisch überexprimierten Gene Nr. 232, 1330, 506, 311 , 1272 und 103 löst in den untersuchten Pankreaskarzinomzelllinien MiaPaCa2 bzw. Panc89 Apoptose aus.The reduced by the use of siRNA expression of pancreatic carcinoma specifically overexpressed genes Nos. 232, 1330, 506, 311, 1272 and 103 triggers apoptosis in the investigated pancreatic carcinoma cell lines MiaPaCa2 and Panc89.

Tabelle 5:Table 5:

Figure imgf000137_0001
Figure imgf000137_0001

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Claims

Patentansprüche claims 1. Ein Verfahren zur in vitro Diagnose eines Pankreaskarzioms, bei dem1. A method for in vitro diagnosis of pancreatic carcinoma, in which 1 ) die Menge an Genprodukt von mindestens einem der in Tabelle 1 und/oder Tabelle 2 aufgeführten Gene in einer Probe biologischen Materials eines Individuums bestimmt wird,1) the amount of gene product of at least one of the genes listed in Table 1 and / or Table 2 is determined in a sample of biological material of an individual, 2) die Menge an Genprodukt in der Probe biologischen Materials mit der Menge des entsprechenden Genprodukts, die in normalem Pankreasepithelgewebe üblicherweise vorliegt, verglichen wird und2) comparing the amount of gene product in the biological material sample with the amount of the corresponding gene product commonly present in normal pancreatic epithelial tissue, and 3) wobei, falls es sich um ein in der Tabelle 1 aufgeführtes Gen handelt, eine erhöhte Menge des3) wherein, if it is a gene listed in Table 1, an increased amount of Genprodukts, falls es sich um ein in der Tabelle 2 aufgeführtes Gen handelt, eine erniedrigte Menge des Genprodukts ein Indiz für das Vorliegen eines Pankreaskarzinoms ist.Gene product, if it is a gene listed in Table 2, a decreased amount of the gene product is an indication of the presence of a pancreatic carcinoma. 2. Verfahren nach Anspruch 1 , dadurch gekennzeichnet, dass zusätzlich die Menge an Genprodukt von mindestens einem der in der Probe biologischen Materials eines Individuums untersuchten Gene in einer Kontrollprobe bestimmt wird.2. The method according to claim 1, characterized in that in addition the amount of gene product of at least one of the examined in the sample of biological material of an individual genes is determined in a control sample. 3. Verfahren nach Anspruch 1 , dadurch gekennzeichnet, dass zusätzlich die Menge an Genprodukt mindestens eines Referenzgens in der Probe biologischen Materials eines Individuums bestimmt wird.3. The method according to claim 1, characterized in that in addition the amount of gene product of at least one reference gene in the sample of biological material of an individual is determined. 4. Verfahren nach Anspruch 1 , dadurch gekennzeichnet, dass zusätzlich4. The method according to claim 1, characterized in that in addition 4) die Menge an Genprodukt von mindestens einem der in Tabelle 3 und/oder Tabelle 4 aufgeführten Gene in der Probe biologischen Materials eines Individuums bestimmt wird,4) the amount of gene product of at least one of the genes listed in Table 3 and / or Table 4 is determined in the sample of biological material of an individual, 5) die Menge an Genprodukt in der Probe biologischen Materials eines Individuums mit der Menge des entsprechenden Genprodukts, die in normalem Pankreasepithelgewebe üblicherweise vorliegt, verglichen wird und5) comparing the amount of gene product in the sample of biological material of an individual with the amount of the corresponding gene product commonly present in normal pancreatic epithelial tissue, and 6) wobei, falls es sich um ein in der Tabelle 3 aufgeführtes Gen handelt, eine erhöhte Menge des6) wherein, if it is a gene listed in Table 3, an increased amount of Genprodukts, falls es sich um ein in der Tabelle 4 aufgeführtes Gen handelt, eine erniedrigte Menge des Genprodukts ein Indiz für das Vorliegen eines Pankreaskarzinoms ist. 5. Verfahren zum Screenen nach Wirkstoffen zur Therapie des Pankreaskarzioms, bei demGene product, if it is a gene listed in Table 4, a decreased amount of the gene product is an indication of the presence of a pancreatic carcinoma. 5. A method for screening for drugs for the treatment of pancreatic carcinoma, in which 1) eine Zelllinie eines pankreatischen duktalen Adenomakarzinoms mit einer zu testenden Substanz kontaktiert wird,1) a cell line of a pancreatic ductal adenoma carcinoma is contacted with a substance to be tested, 2) anschließend die Menge an Genprodukt von mindestens einem der in Tabelle 1 und/oder Tabelle 2 aufgeführten Gene bestimmt2) then determines the amount of gene product of at least one of the genes listed in Table 1 and / or Table 2 3) und die Menge an Genprodukt dann mit der Menge des entsprechenden Genprodukts, die in einer entsprechenden nicht mit der Testsubstanz kontaktierten Zelllinie vorliegt, verglichen wird,3) and the amount of gene product is then compared with the amount of the corresponding gene product present in a corresponding cell line not contacted with the test substance, 4) wobei, falls es sich um ein in Tabelle 1 aufgeführtes Genprodukt handelt, eine gegenüber der unbehandelten Zelllinie erniedrigte Menge des Genprodukts, falls es sich um ein in Tabelle 2 aufgeführtes Genprodukt handelt, eine gegenüber der unbehandelten Zelllinie erhöhte Menge des Genprodukts ein Hinweis darauf ist, dass die Testsubstanz ein potenzieller Wirkstoff zur Therapie des Pankreaskarzinoms ist.4) where, in the case of a gene product listed in Table 1, an amount of the gene product which is lower than the untreated cell line, if it is a gene product listed in Table 2, an increased amount of the gene product compared to the untreated cell line is an indication thereof is that the test substance is a potential drug for the treatment of pancreatic carcinoma. 6. Verfahren nach Anspruch 5, dadurch gekennzeichnet, dass6. The method according to claim 5, characterized in that 5) zusätzlich die Menge an Genprodukt von mindestens einem der in Tabelle 3 und/oder Tabelle 4 aufgeführten Gene bestimmt5) additionally determines the amount of gene product of at least one of the genes listed in Table 3 and / or Table 4 6) und die Menge an Genprodukt dann mit der Menge des entsprechenden Genprodukts, die in einer entsprechenden nicht mit der Testsubstanz kontaktierten Zelllinie vorliegt, verglichen wird,6) and the amount of gene product is then compared with the amount of the corresponding gene product present in a corresponding cell line not contacted with the test substance, 7) wobei, falls es sich um ein in Tabelle 3 aufgeführtes Genprodukt handelt, eine gegenüber der unbehandelten Zelllinie erniedrigte Menge des Genprodukts, falls es sich um ein in Tabelle 4 aufgeführtes Genprodukt handelt, eine gegenüber der unbehandelten Zelllinie erhöhte Menge des Genprodukts ein Hinweis darauf ist, dass die Testsubstanz ein potenzieller Wirkstoff zur Therapie des7) where, in the case of a gene product listed in Table 3, an amount of the gene product which is lower than the untreated cell line, if it is a gene product listed in Table 4, an increased amount of the gene product compared to the untreated cell line is an indication thereof is that the test substance is a potential drug for the therapy of Pankreaskarzinoms ist. 7. Diagnostischer Kit zur Diagnose des Pankreaskarziom mittels RT-PCR, der mindestens folgende Bestandteile enthält: für die cDNA-Synthese: a) Random-Hexamer-Primer oder oligo dT, b) Reverse Transkriptase; für die PCR: a) mindestens zwei Primer, die. spezifisch mit der cDNA mindestens eines der Gene, ausgewählt aus den Gruppen der pankreastumorspezifischen Gene aus den Tabellen Nr. 1 und Nr.2 hybridisieren; b) eine über 8O0C beständige DNA-Polymerase, sowie entsprechende Reaktionspuffer.Pancreatic carcinoma is. 7. Diagnostic kit for the diagnosis of pancreatic carcinoma by RT-PCR, which contains at least the following components: for the cDNA synthesis: a) random hexamer primer or oligo dT, b) reverse transcriptase; for the PCR: a) at least two primers, the. specifically hybridizing with the cDNA at least one of the genes selected from the groups of pancreatic tumor specific genes from Tables Nos. 1 and 2; b) a stable over 8O 0 C DNA polymerase, and corresponding reaction buffer. 8. Diagnostischer Kit nach Anspruch 7, dadurch gekennzeichnet, dass er als zusätzliche Bestandteile für die PCR enthält: c) eine Fluoreszenz-markierte Hybridisierungssonde oder ein Paar Fluoreszenz markierter Hybridisierungssonden, die spezifisch mit dem pankreas¬ tumorspezifischen Gen aus Tabelle Nr. 1 oder Nr.2 hybridisieren.8. Diagnostic kit according to claim 7, characterized in that it contains as additional constituents for the PCR: c) a fluorescence-labeled hybridization probe or a pair of fluorescence-labeled hybridization probes which specifically with the pancreatic tumor-specific gene from Table No. 1 or no .2 hybridize. 9. Verwendung mindestens eines Oligonukleotids, das eine Teilsequenz eines der in den Tabellen 1 und/oder 2 aufgeführten Gene oder deren komplementären Sequenz umfasst, zur Diagnose des Pankreaskarzinoms.9. Use of at least one oligonucleotide which comprises a partial sequence of one of the genes listed in Tables 1 and / or 2 or their complementary sequence for the diagnosis of pancreatic carcinoma. 10. Verwendung mindestens eines Oligonukleotids nach Anspruch 9, dadurch gekennzeichnet, dass zusätzlich mindestens ein Oligonukleotid, das eine Teilsequenz eines der in den Tabellen 3 und/oder 4 aufgeführten Gene oder deren komplementären Sequenz umfasst, zur Diagnose des Pankreaskarzinoms eingesetzt wird.10. Use of at least one oligonucleotide according to claim 9, characterized in that additionally at least one oligonucleotide comprising a partial sequence of one of the genes listed in Tables 3 and / or 4 or their complementary sequence is used for the diagnosis of pancreatic carcinoma. 11. Verwendung eines Gens aus Tabelle 1 oder Tabelle 2 als spezifischer Marker zur Diagnose von Pankreaskarzinom.11. Use of a gene from Table 1 or Table 2 as a specific marker for the diagnosis of pancreatic carcinoma. 12. Verwendung mindestens eines Antisense-Konstrukts, das mindestens 12 Nukleotide einer Sequenz eines Gens umfasst, das in Tabelle 1 aufgeführt ist, wobei die Sequenz bezüglich des Promotors, der ihre Expression kontrolliert, in 3' zu 5' Orientierung vorliegt, für die Herstellung einer pharmazeutischen Zusammensetzung zur Behandlung des Pankreaskarzinoms beim Menschen, wobei das Antisense-Konstrukt den Krebszellen verabreicht wird, so dass das Antisense Konstrukt in den Krebszellen exprimiert wird.12. Use of at least one antisense construct comprising at least 12 nucleotides of a sequence of a gene listed in Table 1, wherein the sequence is in 3 'to 5' orientation with respect to the promoter which controls its expression a pharmaceutical composition for the treatment of pancreatic carcinoma in humans, wherein the antisense construct is administered to the cancer cells so that the antisense construct is expressed in the cancer cells. 13. Verwendung mindestens eines Antisense-Konstrukts nach Anspruch 12, dadurch gekennzeichnet, dass zusätzlich mindestens ein Antisense-Konstrukt, das mindestens 12 Nukleotide einer Sequenz eines Gens umfasst, das in Tabelle 3 aufgeführt ist, wobei die Sequenz bezüglich des Promotors, der ihre Expression kontrolliert, in 3' zu 5' Orientierung vorliegt, eingesetzt wird.13. Use of at least one antisense construct according to claim 12, characterized in that in addition at least one antisense construct comprising at least 12 nucleotides of a sequence of a gene listed in Table 3, wherein the sequence with respect to the promoter, their expression controlled, in 3 'to 5' orientation, is used. 14. Verwendung mindestens eines doppelsträngigen RNA-Konstrukts (siRNA), das mindestens 19 Basenpaare, besonders bevorzugt 21 oder 22 Basenpaare, maximal 29 Basenpaare, einer Sequenz eines Gens umfasst, das in Tabelle 1 aufgeführt ist, für die Herstellung eines Arzneimittels zur Behandlung des Pankreaskarzinoms beim Menschen.14. Use of at least one double-stranded RNA construct (siRNA) comprising at least 19 base pairs, more preferably 21 or 22 base pairs, at most 29 base pairs, of a sequence of a gene listed in Table 1 for the manufacture of a medicament for the treatment of Pancreatic carcinoma in humans. 15. Verwendung mindestens eines doppelsträngigen RNA-Konstrukts (siRNA) nach Anspruch 14, dadurch gekennzeichnet, dass das doppelsträngigen RNA-Konstrukt eine Sequenz eines Gens umfasst, das ausgewählt ist aus den Genen Nr. 232, 506, 311 und 103.15. Use of at least one double-stranded RNA construct (siRNA) according to claim 14, characterized in that the double-stranded RNA construct comprises a sequence of a gene selected from genes Nos. 232, 506, 311 and 103. 16. Verwendung mindestens eines doppelsträngigen RNA-Konstrukts (siRNA) nach Anspruch 14, dadurch gekennzeichnet, dass zusätzlich mindestens ein doppelsträngiges RNA-Konstrukt, das mindestens 19 Basenpaare, besonders bevorzugt 21 oder 22 bp, maximal 29 Basenpaare, einer Sequenz eines Gens umfasst, das in Tabelle 3 aufgeführt ist, eingesetzt wird.16. Use of at least one double-stranded RNA construct (siRNA) according to claim 14, characterized in that additionally comprises at least one double-stranded RNA construct comprising at least 19 base pairs, more preferably 21 or 22 bp, a maximum of 29 base pairs, a sequence of a gene, which is listed in Table 3 is used. 17. Verwendung mindestens eines doppelsträngigen RNA-Konstrukts (siRNA) nach Anspruch 16, dadurch gekennzeichnet, dass das zusätzlich eingesetzte doppelsträngige RNA-Konstrukt eine Sequenz eines Gens umfasst, das ausgewählt ist aus den Genen Nr. 1330 und 1272.17. Use of at least one double-stranded RNA construct (siRNA) according to claim 16, characterized in that the additionally used double-stranded RNA construct comprises a sequence of a gene selected from genes Nos. 1330 and 1272. 18. Verwendung mindestens eines Polynukleotids, das eine Sequenz eines Gens umfasst, das in Tabelle 2 aufgeführt ist, zur Herstellung einer pharmazeutischen Zusammensetzung zur Behandlung des Pankreaskarzinoms beim Menschen, wobei das Polynukleotid den Krebszellen verabreicht wird, so dass das Gen in den Krebszellen exprimiert wird. 18. Use of at least one polynucleotide comprising a sequence of a gene listed in Table 2 for the preparation of a pharmaceutical composition for the treatment of human pancreatic carcinoma, wherein the polynucleotide is administered to the cancer cells such that the gene is expressed in the cancer cells , 19. Verwendung mindestens eines Polynukleotids nach Anspruch 18, dadurch gekennzeichnet, dass zusätzlich mindestens ein Polynukleotid, das eine Sequenz eines Gens umfasst, das in Tabelle 4 aufgeführt ist, eingesetzt wird. 19. Use of at least one polynucleotide according to claim 18, characterized in that additionally at least one polynucleotide comprising a sequence of a gene listed in Table 4 is used.
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