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WO2006024041A3 - Monomeric and dimeric fluorescent protein variants and methods for making same - Google Patents

Monomeric and dimeric fluorescent protein variants and methods for making same Download PDF

Info

Publication number
WO2006024041A3
WO2006024041A3 PCT/US2005/030793 US2005030793W WO2006024041A3 WO 2006024041 A3 WO2006024041 A3 WO 2006024041A3 US 2005030793 W US2005030793 W US 2005030793W WO 2006024041 A3 WO2006024041 A3 WO 2006024041A3
Authority
WO
WIPO (PCT)
Prior art keywords
variants
monomeric
fluorescent protein
methods
fluorescent proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2005/030793
Other languages
French (fr)
Other versions
WO2006024041A2 (en
Inventor
Roger Y Tsien
Robert E Campbell
Nathan C Shaner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/931,304 external-priority patent/US7687614B2/en
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Publication of WO2006024041A2 publication Critical patent/WO2006024041A2/en
Anticipated expiration legal-status Critical
Publication of WO2006024041A3 publication Critical patent/WO2006024041A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates generally to fluorescent proteins and fluorescent protein variants, and more specifically to monomeric and dimeric forms of Anthozoan fluorescent proteins. In one aspect, the present invention provides variants of fluorescent proteins, where the variants have a reduced propensity to tetramerize, and form dimeric or monomeric structures. In a further aspect, the present invention provides variants of fluorescent proteins, the variants being characterized by more efficient maturation than corresponding fluorescent proteins from which they are derived. The invention also relates to methods of making and using such fluorescent proteins and fluorescent protein variants, including fluorescent protein monomers and dimers.
PCT/US2005/030793 2004-08-27 2005-08-26 Monomeric and dimeric fluorescent protein variants and methods for making same Ceased WO2006024041A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US92936204A 2004-08-27 2004-08-27
US10/929,362 2004-08-27
US10/931,304 2004-08-30
US10/931,304 US7687614B2 (en) 2001-02-26 2004-08-30 Monomeric and dimeric fluorescent protein variants and methods for making same

Publications (2)

Publication Number Publication Date
WO2006024041A2 WO2006024041A2 (en) 2006-03-02
WO2006024041A3 true WO2006024041A3 (en) 2007-07-26

Family

ID=35968346

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/030793 Ceased WO2006024041A2 (en) 2004-08-27 2005-08-26 Monomeric and dimeric fluorescent protein variants and methods for making same

Country Status (1)

Country Link
WO (1) WO2006024041A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008024567A1 (en) * 2008-05-21 2009-12-03 Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts Universitätsmedizin Method for measuring concentration of measuring substance in biological sample using molecular fluorescence resonance energy transfer-biosensors, involves irradiating sample with excitation light of spectral region
KR101834574B1 (en) 2009-01-29 2018-03-05 커먼웰쓰 사이언티픽 앤드 인더스트리얼 리서치 오가니제이션 Measuring g protein coupled receptor activation
CN108164591B (en) * 2013-08-06 2022-12-20 佐治亚州立大学研究基金会公司 Metal ion sensor and method for detecting metal ions
CN109239037B (en) * 2018-09-28 2021-03-26 长沙理工大学 Biosensors based on MOFs as energy receptors and their preparation methods and applications

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000034326A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
US6852489B2 (en) * 2000-09-21 2005-02-08 National Food Research Institute Simple and quick method for determining the nucleotide sequence of a mitochondrial 21S ribosomal RNA gene of yeast belonging to Saccharomyces cerevisiae
US7005511B2 (en) * 2001-02-26 2006-02-28 The Regents Of The University Of California Fluorescent protein variants and methods for making same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000034326A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
US6852489B2 (en) * 2000-09-21 2005-02-08 National Food Research Institute Simple and quick method for determining the nucleotide sequence of a mitochondrial 21S ribosomal RNA gene of yeast belonging to Saccharomyces cerevisiae
US7005511B2 (en) * 2001-02-26 2006-02-28 The Regents Of The University Of California Fluorescent protein variants and methods for making same

Also Published As

Publication number Publication date
WO2006024041A2 (en) 2006-03-02

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