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WO2006002452A1 - Procede et dispositif de spectroscopie raman - Google Patents

Procede et dispositif de spectroscopie raman Download PDF

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Publication number
WO2006002452A1
WO2006002452A1 PCT/AT2005/000249 AT2005000249W WO2006002452A1 WO 2006002452 A1 WO2006002452 A1 WO 2006002452A1 AT 2005000249 W AT2005000249 W AT 2005000249W WO 2006002452 A1 WO2006002452 A1 WO 2006002452A1
Authority
WO
WIPO (PCT)
Prior art keywords
ultrasonic
laser beam
target material
standing wave
wave field
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/AT2005/000249
Other languages
German (de)
English (en)
Inventor
Bernhard Lendl
Stefan Radel
Ewald Benes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Innovationsagentur GmbH
Original Assignee
Innovationsagentur GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Innovationsagentur GmbH filed Critical Innovationsagentur GmbH
Priority to EP05754158A priority Critical patent/EP1763657A1/fr
Publication of WO2006002452A1 publication Critical patent/WO2006002452A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N2021/651—Cuvettes therefore
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N2021/653—Coherent methods [CARS]
    • G01N2021/656—Raman microprobe

Definitions

  • the invention relates to a method for carrying out RAMAN spectroscopy, wherein a laser beam is directed in a measuring range to a sample which comprises a solid or liquid target material dispersed or suspended in a carrier liquid, e.g. organic material, and a radiation then emitted from the sample is detected for that sample.
  • a carrier liquid e.g. organic material
  • the invention relates to a device for performing RAMAN spectroscopy, with a laser device and with a RAMAN spectrometer, and with optical elements for directing the laser beam of the laser device to a position in a measuring range.
  • Raman spectroscopy is used in particular in the analysis of small organic droplets or particles in a carrier liquid, such as water, and it is based on the fact that laser radiation, in particular in the near infrared region, is generated and applied to the droplet to be analyzed Particle is focused, in which case from this target material, a specific frequency-shifted radiation is emitted, which is supplied to the RAMAN spectrometer. Corresponding filters are used to remove an unwanted Rayleigh radiation which is likewise present.
  • RAMAN spectroscopy in which, moreover, the droplets or particles to be analyzed are fixed in the water by means of the radiation pressure of the laser radiation, is described, for example, in the article: Katsuhiro Ajito Combined Near-Infrared Raman Microprobe and Laser Trapping System: Application to the Analysis of a Single Organic Microdroplet in Water, Applied Spectroscopy, Vol. 52, No. 3, 1998, pp. 339-342; in the article: Katsuhiro Ajito et al, Investigation of the Molecular Extraction Process in Single Subpicoliter Droplets Using a Near-Infrared Laser Radar Trapping System ", Analytical Chemistry, Vol. 72, No. 19, Oct. 1, 2000, pp.
  • the droplets In this known RAMAN spectroscopy, therefore, the droplets, generally the target material, are captured and fixed with the aid of the laser radiation itself, which however is only relatively unreliable, especially when larger, heavier particles or particle agglomerates are involved. On the other hand, such larger materials or agglomerates, if present, are naturally favorable for spectroscopy, since then the received radiation is more intense and can be detected more easily. Also, it is problematic in the known technique to capture the droplets or particles at all in the laser light, specifically at the focal point of the laser beam, since their location is not known from the outset.
  • a further object of the invention is to propose a technique with which not only the actual target material itself, but also the carrier liquid can be reliably or exactly measured so as to be able to measure it also by way of example. to be able to make statements about the concentration of certain substances in it.
  • the invention provides a method and a device as defined in the appended independent claims.
  • Advantageous embodiments and further developments are indicated in the respective subclaims.
  • the target materials ie, the particles or droplets to be analyzed
  • the target materials are concentrated and captured by generating an ultrasonic standing wave field in zones defined by this ultrasonic standing wave field, specifically in the pressure or fast node levels of the standing wave ultrasonic field.
  • Such ultrasonic vibrators in the form of so-called “quasi-standing waves", can do well be controlled, in the case of the use of piezoelectric transducers (piezo transducers) as ultrasonic transmitter, as is preferably provided, the ultrasonic field simply by appropriate adjustment of the electrical signal, which is used to An ⁇ control of the piezoelectric transducer, with respect to Fre ⁇ frequency and amplitude can be controlled.
  • the emitted ultrasonic wave can be reflected on the opposite side of the ultrasonic transmitter with the aid of an ultrasonic reflector, the returning wave is then superimposed über ⁇ the radiated wave, so that the desired standing wave is formed.
  • the envelope of the amplitude in the direction of the direction of sound propagation is stationary, that is to say temporally constant.
  • the ultrasonic standing wave field can also be obtained by arranging two ultrasonic transmitters opposite one another, wherein the ultrasonic waves emitted by the two ultrasonic transmitters are superimposed on one another and thus lead to the standing wave field or to the "quasi-standing waves" ,
  • axial primary radiation forces act on the actual target material contained in a carrier liquid, namely droplets or particles, the effect on the target material, eg cells, for example yeast cells, being such that this target material is in the direction of the pressure nodes the standing wave field is urged.
  • the target material eg cells, for example yeast cells
  • it depends on the properties of the particle or droplet material (density, speed of sound or compressibility, possibly also viscosity) and the corresponding properties of the carrier liquid, whether a droplet or particle is pressed into the pressure or fast knots.
  • the target material present in the carrier liquid in the measuring region is parallel to the piezos in the measuring region.
  • the target material Since, as a rule, the ultrasound field is stronger in the piezo-transducer, for example in the middle, than at the edge because of the not entirely homogeneous ultrasound generation, the target material also acts on the transducer surface transversal primary Sonic radiation forces, which, after concentration of the target material in the pressure or fast node planes, results in forces being exerted on the target material in the direction of an axis, eg center axis, of the measuring range within these levels, resulting in increased agglomeration the target material comes at the interfaces of this axis with the pressure or Schnelle ⁇ node levels; As a result, a kind of chain of agglomerates in the measuring range is obtained.
  • the laser beam can be directed or "focused" on this concentrated target material or on these agglomerates without problems, since in principle the location of the pressure and fast knot planes or of the agglomerate is known, and the concentration of the agglomerates remains so long
  • the ultrasonic field is deactivated, the agglomerates are released and, for example, in the case of a fluid flow through the measuring range, are transported out of the measuring range by this "flow".
  • RAMAN spectroscopy advantageously permits the monitoring of biotechnological processes, such as fermentations.
  • the aim of the monitoring is to know the current state of this process at any time.
  • bio chemical parameters are also important here. These include: the concentration of solutes (e.g., glucose, ammonium, amino acids, organic acids, etc.); but also information about solid constituents, the biocatalysts (microorganisms, eg yeast) themselves.
  • solutes e.g., glucose, ammonium, amino acids, organic acids, etc.
  • biocatalysts microorganisms, eg yeast
  • the ultrasonic standing wave field can be generated, for example, in such a way that the pressure node planes are at least substantially perpendicular to the laser beam direction, and in the case of the generation of several pressure node planes in the measuring range, it is expedient in this case to carry out the spectroscopy if the laser beam eg is focused on the closest target material or the nearest print or fast node level containing target material.
  • the ultrasonic transmitter is preferably arranged on the side of the measuring range facing away from the entrance side of the laser beam, so that the emission direction of the ultrasonic transmitter essentially opposite to the direction of the laser beam.
  • the ultrasonic standing wave field is generated with the emission of ultrasound waves at least substantially perpendicular to the direction of the laser beam, i. the ultrasonic transmitter is arranged with its emission direction substantially transversely to the laser beam direction.
  • the pressure node planes (as well as the intermediate pressure levels, ie fast node planes) essentially run parallel to the laser beam direction, and the laser beam enters the measuring range between the ultrasonic transmitter and an ultrasonic reflector opposite thereto or further ultrasonic transmitter one.
  • the focal point of the laser beam can also be controlled simply relative to a nodal plane of the ultrasonic standing wave field, that the frequency of the ultrasound is changed, whereby the node levels of the ultrasound are changed. shift accordingly standing wave field.
  • This can be observed via a microscope or can be carried out up to a maximum detector signal, in which case the focal point is present in a nodal plane, so that then the particles or droplets are optimally detected and excited by the laser beam.
  • the excitation laser beam is intentionally directed at locations adjacent to such particles (droplets).
  • a frequency adjusting unit is preferably connected to the ultrasound transmitter for changing the frequency of the ultrasound, which in the case of a piezo transducer is simply a setting unit for the frequency of the electrical signal which is applied to the - e.g. ceramic piezo element is applied.
  • the piezoelectric element can simply be mounted on a small plate, in particular a simple glass plate, for example by gluing; If an ultrasound reflector is used, it can also be realized by a glass plate.
  • the measuring range can be formed by a closed measuring cell or else by a flow cell.
  • the present spectroscopy technique can be applied with particular advantage directly to process vessels, such as fermentation vessels, for ongoing process monitoring;
  • the device can therefore be fixed as a kind of "probe" to the wall of such a container, being present with the measuring area inside the container
  • the ultrasonic transmitter and the ultrasonic reflector to be formed by discontinuous wall elements or rods
  • the cage-type measuring cell ensures that there is constant exchange of the medium in the container or in the measuring area, so that always up-to-date spectroscopy measurements can be carried out.
  • the laser radiation can be easily supplied to the measuring range via optical fibers.
  • the laser beam itself is focused at the location of the target material (or next to it) to an order of magnitude of, for example, 2-5 ⁇ m, and it is thus possible, for example, to obtain RAMAN spectra of individual bacteria as the target material.
  • the laser device can be constructed in a conventional manner, wherein it is also conceivable to connect a pump laser with a Ti: sapphire (Ti: Al 2 O 3 ) laser to a specific wavelength (eg of the order of magnitude from 200-1200 nm).
  • a pump laser with a Ti: sapphire (Ti: Al 2 O 3 ) laser to a specific wavelength (eg of the order of magnitude from 200-1200 nm).
  • a specific wavelength eg of the order of magnitude from 200-1200 nm.
  • other laser devices such as krypton laser, He-Ne laser or Nd: YAG laser, etc., can be used.
  • FIG. 1 schematically shows the structure of a device for RAMAN spectroscopy including arrangement for generating an ultrasonic Steh ⁇ wave field in the measuring range;
  • Fig. 2 in a comparable representation as in Figure 1 the part of the measuring range with a modified arrangement for generating the ultrasonic standing wave field.
  • FIG. 3 shows in a diagram three curves of the intensity of the detector signal (in arbitrary unit) versus the wavenumber (in cm -1 ), wherein spectroscopy measurement results for a target material suspended in a carrier liquid, for a carrier liquid (without target material) and are illustrated for a target material without carrier liquid;
  • Fig. 1 is a device 1 for carrying out schematically of RAMAN spectroscopy measurements, only very schematically, within a border 2, which more particularly shows such a RAMAN spectroscope, a laser device 3 and a spectrometer 4 with detector 5 (for example in the form of a CCD). Camera) and another detector 6 (in particular also in the form of a CCD camera) are arranged.
  • detector 5 for example in the form of a CCD
  • Camera another detector 6 (in particular also in the form of a CCD camera) are arranged.
  • detector 5 for example in the form of a CCD
  • Camera detector 6
  • FIG. 1 a device 1 for carrying out schematically of RAMAN spectroscopy measurements, only very schematically, within a border 2, which more particularly shows such a RAMAN spectroscope, a laser device 3 and a spectrometer 4 with detector 5 (for example in the form of a CCD). Camera) and another detector 6 (in particular also in the form of a CCD camera) are
  • a focusing lens 9 for focusing the laser beam 10 in a focal point 11 in a measuring area 12 is symbolically represented.
  • the laser beam 10 can be fed to the measuring area 12 via one or more (preferably three), only very schematically indicated light guides 13, 13 'in FIG.
  • the position of the laser focal point 11 in the measuring region 12 can then be achieved, for example, by illumination of the respectively favorable optical waveguide 13, 13 '.
  • a further adjustment of the focal point 11 is that in the depth, ie according to FIG. 1 (and 2) upwards or downwards, and this can be accomplished via the focus optics indicated by the lens 9.
  • a control unit 14 is further shown schematically, which will generally be formed essentially by a computer device, and connected to the laser device 3, the RAMAN spectrometer 4 together with the detector 5 and the other De ⁇ detector 6 is.
  • This arrangement 15 contains an ultrasound transmitter 16, which, for example, has a ceramic piezoelement 17 which is adhesively bonded to a glass plate 18.
  • a glass plate 18 facing flat electrode 19 is provided, which consists for example of silver, and which is pulled over the edges of the piezoelectric element 17 to the lower in Fig. 1 outer side of the piezoelectric element 17 and there kontak ⁇ at 20 kontak ⁇ .
  • a second, central electrode 21 is also located on the lower outer side of the piezoelectric element 17 and is contacted there directly at 22.
  • the two contacts 20, 22 are connected to a frequency generator 23, such as a frequency synthesizer FPS 4025, to generate a vibration spielmud in the range of 1.7 MHz to 1.8 MHz.
  • the electrical signal generated by the frequency generator 19 with this frequency is applied via the contacts 20, 22 to the piezoelectric element 17, ie at its electrodes 19, 21, so that the ultrasound transmitter 16 a corresponding ultrasonic wave with the frequency of 1.7 MHz emitted up to 1.8 MHz.
  • this ultrasonic wave is emitted vertically upward through the measuring area 12 and at the upper side of the measuring area, where the laser beam 10 enters the measuring area 12, from an ultrasonic reflector 25 formed by another glass plate 24 reflected.
  • the number of pressure node levels 26 in the measuring range 12 depends on the Size (height) of the measuring range 12 as well as the wavelength of the ultrasound in the measuring range (ie the medium present there), and it is conceivable that depending on the speed of sound in the measuring range 12 and depending on the frequency of the ultrasonic field half the wavelength as a distance between two adjacent Pressure node levels 26 in of the order of 0.3 mm or 0.4 mm; this would lead to a good ten pressure node planes 26 within the measuring area 12 at a height of the measuring area 12 of approximately 4 mm.
  • the measuring region 12 may have a cylindrical shape, wherein the surfaces of the ultrasonic transmitter 16 or reflector 25 are essentially circular, but the measuring region 12 may of course also be different, for example cuboidal or cuboidal, with a rectangular or rectangular top view quadratic piezo transducer 16 and ultrasonic reflector 25, respectively.
  • nodal planes 26 particles or droplets present in a carrier liquid, in short target material, eg in the pressure node planes 26 or as mentioned in the sound velocity node planes (hereinafter, therefore, are only briefly referred to nodal planes 26) are collected, with the agglomerates 27 thus formed in the Knotenbenen 26 in turn konzen ⁇ due to the effect of transversal primary sound radiation forces in the region of an axis.
  • the transversal primary sound radiation forces which are responsible for the movement of the target material in the node planes 26 are mainly due to the fact that in ultrasonic transducers in FIG As a rule, the ultrasound field is inhomogeneous with respect to the surface of the ultrasound emission of the ultrasound transducer, wherein it is stronger, especially in a central region, than in peripheral regions. Overall, a "chain" of agglomerates 27, as shown in FIG.
  • a corresponding frequency setting unit 28 is provided in the control unit 14.
  • this Frequenzenzeinstellatti 28 may also be part of the frequency generator 23 directly and operated there immedi.
  • the laser beam 10 it is also possible with the laser beam 10 to focus specifically on locations next to the particles or droplets 27, so that a sample, namely now the carrier liquid, without these particles or droplets 27 is certainly measured.
  • the glass plate 24 forming the ultrasound reflector 25 can be connected to the glass plate 18 of the ultrasound transducer or transmitter 16 via a wall 29 which laterally delimits the measuring area 12, in which way a measuring cell 30 is formed.
  • This measuring cell 30 can in principle be designed in the form of a flow-through cell, wherein corresponding Zuleitun ⁇ gene and derivatives are provided, as is schematically illustrated in Fig. 1 by dashed lines 31 and 32 respectively.
  • the entire device 1 may be connected to a production plant, such as a fermentation tank for process monitoring.
  • a production plant such as a fermentation tank for process monitoring.
  • the present device 1 can advantageously be fed directly to a production process for continuous process monitoring.
  • container such as a fermentation tank, are used, whereby it is only necessary to shield the required electrical lines and the ultrasonic transducer 16 accordingly housed in the container.
  • FIG. 2 illustrates a modified arrangement of the measuring cell 30 or the ultrasonic elements compared to FIG.
  • a probe-like design of the device 1 for mounting inside a production container indicated by a wall 33 is again provided, wherein a transparent wall 34, in the form of a glass plate, with a Laserstrahlzut ⁇ tion, about once again by means of one or more light guide 13th (see Fig. 1) enclosing housing 35 is connected.
  • the focal point (11 in FIG. 1) “laterally” and “vertically” (as shown in FIG. 2) with the aid of the correspondingly activated or displaced light guides, in order to provide the desired locations - agglomerate at pressure nodes or fast knots or carrier liquid - reach.
  • the ultrasonic standing wave field is generated transversely to the direction of the laser beam 10, ie, node planes 26 that run essentially parallel to the direction of the laser beam 10 result.
  • the ultrasonic standing wave field is generated here, for example, with the aid of two ultrasonic transmitters or transducers 16, 16 ', each of which may be designed, for example, as explained above with reference to the ultrasonic transducer 16 according to FIG. 1, and the ultrasonic waves in opposite directions emit, which are superimposed to the desired standing wave field.
  • the laser beam 10 is focused here, for example, on an agglomerate 27 in a central nodal plane 26 (or on a location next to it).
  • the corresponding nodal plane 26 can in turn here by changing the frequency of the electrical signal to the piezoelectric elements 17, 17 'of the ultrasonic transducers 16 and 16' as shown in Fig. 2 are shifted to the left or right, so as to the agglomerate 27 in optimal spatial To bring relationship to the focal point 11 of the laser beam 10.
  • the ultrasound transducers 16, 16 ', more precisely their glass platelets 18, 18' can in turn be connected via lattice-type or rod-like elements, which form the remaining wall 29 of the measuring cell 30, mitein ⁇ and be connected to the upper glass plate 34, so as to likewise obtain a cage-like measuring cell 30, which is in flow connection with its interior with the environment.
  • curve A illustrates a spectroscopy measurement with a Raman spectrometer using an ultrasonic Stehwellen ⁇ field as explained with reference to Fig.

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  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne un procédé et un dispositif (1) de spectroscopie Raman, selon laquelle un faisceau laser (10) se trouvant dans une zone de mesure (12) est dirigé vers un échantillon, lequel contient un matériau cible solide ou liquide, en dispersion ou en suspension dans un liquide support, par ex. une matière organique, un rayonnement spécifique émis par cet échantillon étant ensuite déterminé. Selon l'invention, un champ d'ondes ultrasonores stationnaires est généré dans la zone de mesure (12), le matériau cible solide ou liquide, tel que des cellules de levure, étant maintenu, éventuellement après agglomération, dans des plans de pression (26) ou nodaux rapides du champ d'ondes ultrasonores stationnaires, et le faisceau laser (10) étant dirigé vers le matériau cible solide ou liquide ainsi maintenu et/ou vers des emplacements voisins du matériau cible solide ou liquide.
PCT/AT2005/000249 2004-07-05 2005-07-05 Procede et dispositif de spectroscopie raman Ceased WO2006002452A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP05754158A EP1763657A1 (fr) 2004-07-05 2005-07-05 Procede et dispositif de spectroscopie raman

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT11312004A AT413446B (de) 2004-07-05 2004-07-05 Verfahren und einrichtung zum durchführen von raman-spektroskopie
ATA1131/2004 2004-07-05

Publications (1)

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WO2006002452A1 true WO2006002452A1 (fr) 2006-01-12

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PCT/AT2005/000249 Ceased WO2006002452A1 (fr) 2004-07-05 2005-07-05 Procede et dispositif de spectroscopie raman

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EP (1) EP1763657A1 (fr)
AT (1) AT413446B (fr)
WO (1) WO2006002452A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116297386A (zh) * 2023-01-29 2023-06-23 中国农业大学 一种瘦肉精残留检测用拉曼光谱精准高效采集装置
CN120489964A (zh) * 2025-07-16 2025-08-15 天津理工大学 一种红外光谱检测方法及其检测用超声驻波样品池

Citations (4)

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Publication number Priority date Publication date Assignee Title
WO1994020833A1 (fr) * 1993-03-05 1994-09-15 University College London Procede et appareil de manipulation, de positionnement et d'analyse de particules en suspension
EP0773055A2 (fr) * 1995-11-08 1997-05-14 Hitachi, Ltd. Méthode et appareil pour traiter des particules par rayonnement acoustique
WO2000001295A1 (fr) * 1998-07-07 2000-01-13 Lightouch Medical, Inc. Processus de modulation tissulaire destine a l'analyse spectroscopique quantitative non invasive in vivo des tissus
US20030049642A1 (en) * 2001-01-19 2003-03-13 Staffan Nilsson Screening system

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7846382B2 (en) * 2002-06-04 2010-12-07 Protasis Corporation Method and device for ultrasonically manipulating particles within a fluid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994020833A1 (fr) * 1993-03-05 1994-09-15 University College London Procede et appareil de manipulation, de positionnement et d'analyse de particules en suspension
EP0773055A2 (fr) * 1995-11-08 1997-05-14 Hitachi, Ltd. Méthode et appareil pour traiter des particules par rayonnement acoustique
WO2000001295A1 (fr) * 1998-07-07 2000-01-13 Lightouch Medical, Inc. Processus de modulation tissulaire destine a l'analyse spectroscopique quantitative non invasive in vivo des tissus
US20030049642A1 (en) * 2001-01-19 2003-03-13 Staffan Nilsson Screening system

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1763657A1 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116297386A (zh) * 2023-01-29 2023-06-23 中国农业大学 一种瘦肉精残留检测用拉曼光谱精准高效采集装置
CN120489964A (zh) * 2025-07-16 2025-08-15 天津理工大学 一种红外光谱检测方法及其检测用超声驻波样品池

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EP1763657A1 (fr) 2007-03-21
ATA11312004A (de) 2005-07-15
AT413446B (de) 2006-02-15

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