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WO2006097347A3 - Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus - Google Patents

Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus Download PDF

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Publication number
WO2006097347A3
WO2006097347A3 PCT/EP2006/002612 EP2006002612W WO2006097347A3 WO 2006097347 A3 WO2006097347 A3 WO 2006097347A3 EP 2006002612 W EP2006002612 W EP 2006002612W WO 2006097347 A3 WO2006097347 A3 WO 2006097347A3
Authority
WO
WIPO (PCT)
Prior art keywords
identifying
detecting
vaccine strains
methods
differentiating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2006/002612
Other languages
French (fr)
Other versions
WO2006097347A2 (en
Inventor
Ignacio Lopez-Goni
Yoldi David Garcia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Instituto Cientifico y Tecnologico de Navarra SA
Original Assignee
Instituto Cientifico y Tecnologico de Navarra SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instituto Cientifico y Tecnologico de Navarra SA filed Critical Instituto Cientifico y Tecnologico de Navarra SA
Publication of WO2006097347A2 publication Critical patent/WO2006097347A2/en
Publication of WO2006097347A3 publication Critical patent/WO2006097347A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The method for detecting genus, identifying and differentiating species and vaccine strains of the Brucella is based on the amplification, by means of multiplex PCR, of selected genomic DNA fragments corresponding to genomic regions of interest. Said method allows detecting, differentiating and identifying the strains of B. abortus, B. melitensis, B. ovis, B. suis, B. neotomae, B. canis, the isolates of marine mammal Brucella, and the vaccine strains B. abortus S19 (B19), B. abortus RB51 and B. melitensis Rev 1.
PCT/EP2006/002612 2005-03-17 2006-03-16 Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus Ceased WO2006097347A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
ESP200500629 2005-03-17
ES200500629 2005-03-17
ES200503083 2005-12-14
ESP200503083 2005-12-14

Publications (2)

Publication Number Publication Date
WO2006097347A2 WO2006097347A2 (en) 2006-09-21
WO2006097347A3 true WO2006097347A3 (en) 2007-01-04

Family

ID=36930123

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/002612 Ceased WO2006097347A2 (en) 2005-03-17 2006-03-16 Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus

Country Status (1)

Country Link
WO (1) WO2006097347A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3543362B1 (en) * 2013-10-07 2021-09-08 Mitsui Chemicals, Inc. Pcr primer set for bacterial dna amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species
CN105713967B (en) * 2016-01-29 2020-06-16 江汉大学 A qualitative and quantitative detection method for microorganisms in water
RU2621864C1 (en) * 2016-09-19 2017-06-07 Федеральное казенное учреждение здравоохранения "Российский научно-исследовательский противочумный институт "Микроб" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека ("РосНИПЧИ "Микроб") Method for brucellosis pathogen species identification by pcr method with hybridization-fluorescent results accounting in real time
CN108588247A (en) * 2018-05-02 2018-09-28 杭州优思达生物技术有限公司 A kind of kit and its detection method of brucella nucleic acid
CN111733217A (en) * 2019-10-09 2020-10-02 中国疾病预防控制中心传染病预防控制所 A kind of digital PCR detection method of Brucella nucleic acid DNA
CN111593048B (en) * 2020-05-27 2024-04-19 新疆畜牧科学院兽医研究所（新疆畜牧科学院动物临床医学研究中心） Primer combination, kit, amplification method and application in A19 vaccine detection
CN113186312B (en) * 2021-05-03 2022-10-04 中国动物卫生与流行病学中心 Molecular marker for distinguishing Brucella A19 vaccine strain and wild strain
CN115786541B (en) * 2022-07-14 2023-06-27 天津健博生物科技有限公司 SNP molecular marker, primer probe, kit, method and application for identifying Brucella vaccine strain A19
CN116042879B (en) * 2023-01-30 2024-11-26 内蒙古大学 A kit and a detection method for detecting wild strains and vaccine strains of Brucella
CN116590436B (en) * 2023-03-15 2024-02-06 中国农业科学院北京畜牧兽医研究所 RT-PCR kit for identifying Brucella vaccine strain Rev.1

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2078174A1 (en) * 1993-12-16 1995-12-01 Univ Cantabria Molecular diagnostic methods for the detection of Brucella abortus and differentiation from vaccine strain B19

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2078174A1 (en) * 1993-12-16 1995-12-01 Univ Cantabria Molecular diagnostic methods for the detection of Brucella abortus and differentiation from vaccine strain B19

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BRICKER BETSY J ET AL: "Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 32, no. 11, 1994, pages 2660 - 2666, XP002398170, ISSN: 0095-1137 *
BRICKER BETSY J ET AL: "Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 33, no. 6, 1995, pages 1640 - 1642, XP002398169, ISSN: 0095-1137 *
BRICKER BETSY J: "PCR as a diagnostic tool for brucellosis.", VETERINARY MICROBIOLOGY, vol. 90, no. 1-4, 20 December 2002 (2002-12-20), pages 435 - 446, XP002398173, ISSN: 0378-1135 *
CLOECKAERT A ET AL: "Identification of Brucella melitensis vaccine strain Rev.1 by PCR-RFLP based on a mutation in the rpsL gene", VACCINE, BUTTERWORTH SCIENTIFIC. GUILDFORD, GB, vol. 20, no. 19-20, 7 June 2002 (2002-06-07), pages 2546 - 2550, XP004359686, ISSN: 0264-410X *
FERRAO-BECK LENIA ET AL: "Development of a multiplex PCR assay for polymorphism analysis of Brucella suis biovars causing brucellosis in swine", VETERINARY MICROBIOLOGY, vol. 115, no. 1-3, June 2006 (2006-06-01), pages 269 - 277, XP002398172, ISSN: 0378-1135 *
GARCÍA-YOLDI D ET AL: "Multiplex PCR assay for the identification and differentiation of all Brucella species and the vaccine strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1 [13]", CLINICAL CHEMISTRY 2006 UNITED STATES, vol. 52, no. 4, 2006, pages 779 - 781, XP009072107, ISSN: 0009-9147 *
PROBERT W S ET AL: "Real-Time Multiplex PCR Assay for Detection of Brucella spp., B. abortus, and B. melitensis", JOURNAL OF CLINICAL MICROBIOLOGY 2004 UNITED STATES, vol. 42, no. 3, 2004, pages 1290 - 1293, XP002398167, ISSN: 0095-1137 *
SREEVATSAN S ET AL: "Molecular detection of Brucella abortus and/or Mycobacterium bovis infection in cattle", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 100, 2000, & 100TH GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY; LOS ANGELES, CALIFORNIA, USA; MAY 21-25, 2000, pages 139, XP009072077, ISSN: 1060-2011 *
VEMULAPALLI RAMESH ET AL: "Identification of an IS711 element interrupting the wboA gene of Brucella abortus vaccine strain RB51 and a PCR assay to distinguish strain RB51 from other Brucella species and strains", CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, vol. 6, no. 5, September 1999 (1999-09-01), pages 760 - 764, XP002398166, ISSN: 1071-412X *

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