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WO2006084964A1 - Phytosanitary composition used as an elicitor of natural defenses of plants - Google Patents

Phytosanitary composition used as an elicitor of natural defenses of plants Download PDF

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Publication number
WO2006084964A1
WO2006084964A1 PCT/FR2005/050480 FR2005050480W WO2006084964A1 WO 2006084964 A1 WO2006084964 A1 WO 2006084964A1 FR 2005050480 W FR2005050480 W FR 2005050480W WO 2006084964 A1 WO2006084964 A1 WO 2006084964A1
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Prior art keywords
composition according
plants
active compound
compounds
elicitor
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Ceased
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PCT/FR2005/050480
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French (fr)
Inventor
Christelle Martinez
Michel Delrieu
Lucien Delpech
Thierry Clergue
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Occitane De Fabrications Et De Technologies Ayant Pour Sigle Soft Ste
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Occitane De Fabrications Et De Technologies Ayant Pour Sigle Soft Ste
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Publication of WO2006084964A1 publication Critical patent/WO2006084964A1/en
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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40Liliopsida [monocotyledons]
    • A01N65/44Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/34Nitriles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N49/00Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group, wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group, wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/20Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia

Definitions

  • the subject of the present invention is a phytosanitary composition used as elicitor of the natural defenses of plants against fungal and / or bacterial and / or viral and / or pest pathogenic agents by foliar, root or injection application.
  • Such substances are, for example, defined, during fungal attacks, by oligosaccharides emanating, on the one hand, from the degradation of the cell walls of the fungus by the hydrolases of the host plant, and on the other hand by the walls of the fungi. cells of the host plant, themselves hydrolysed by the enzymes of the fungus. These wall debris also serve as messengers of the resistance.
  • the plant develops a series of cellular reactions, including the production of molecules to enhance the strength of cell walls, but also plant antibiotics such as phytoalexins or defense proteins. These molecules have antifungal and antibacterial properties.
  • French patent application No. No. 2,766,059 describes in particular a process which provides for the use of an effective amount of a ⁇ 1-3 (1-6) glucan oligo.
  • This is prepared from ⁇ 1-3 glucans of various origin which are hydrolysed to oligo ⁇ 1-3 glucans.
  • the ⁇ 1-3 glucans come from bacteria, especially Alcaligenes faecalis, fungi, especially Schizophyllum and Sderotium glucanium, yeasts, in particular Saccharomyces cerevisae, algae or cereals.
  • this molecule By triggering a signal of aggression in the plant, this molecule prevents the development of many fungi, viruses and bacteria and thus allow tomatoes, wheat, barley, rice, beans and vines to better defend themselves against their aggressors, significantly reducing quantities of fungicides needed.
  • the method described herein is to apply an elicitor, defined by a polypeptide or protein, to a plant or seed under conditions effective for controlling insects.
  • the preferred elicitor is obtained from a pathogen selected from the group consisting of Erwinia, Pseudomonas, Xanthomonas, Phyrophtora and mixtures thereof.
  • the elicitor is applied to the plants through a bacterium containing genes capable of secreting or exporting the polypeptide or the protein.
  • This document therefore describes a method for allowing a specific control directed against insects and not against the pathogens as a whole. Moreover, the method has the disadvantage of integrating genetic manipulations which are generally very poorly perceived by the public.
  • the document FR 2 832 409 describes the use, as elicitors of the natural defenses of plants, of cyclic or helical oligopeptides, obtained by organic or enzymatic synthesis, and intended to be applied at leaf level, roots or by injection.
  • the present application relates to a new invention in the field of stimulating the natural defenses of plants, namely a phytosanitary composition used as elicitor of the natural defenses of plants against fungal pathogens, and / or bacterial, and / or viral and / or pests, by foliar, root or injection application, characterized in that it contains at least one compound alone or in combination with compounds selected from the following compounds or families: trigonelline, 4-hydroxy-isoleucine, saponins, steroidal and triterpenic sapogenins, nicotinic acid.
  • said compounds are defined by molecules obtained from natural extracts of plants, in particular fenugreek seeds, coffee, oats, verbena, soya beans, borage seeds, orthosyphon.
  • composition according to the invention to be advantageously in line with the current concerns in the field of environmental protection, said extracts being entirely biodegradable and not harmful to the ecosystem.
  • the invention provides yet another feature of said composition according to which said compounds are defined by molecules obtained by chemical synthesis.
  • the sapogenin family compound is defined by diosgenin, extracted from fenugreek seeds.
  • compositions are still defined by the fact that it also contains proteins extracted from fenugreek seeds.
  • the present composition also contains a fungicidal agent, and / or a bactericidal agent, and / or an insecticidal agent, and / or nutrients for the plants.
  • composition according to the invention can be in solid form, for example in powder form, or granules, containing the active compound (s), alone or in combination with other formulating agents.
  • the present composition may be in liquid form, in which case the active compound (s) may be combined with wetting, dispersing and / or adhesive agents.
  • Example Treatment of vegetable and ornamental plants with a composition according to the invention.
  • composition is sprayed on the various plants tested, and their resistance to various parasites is verified.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Insects & Arthropods (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a phytosanitary composition used as an elicitor of natural defenses of plants against fungal and/or bacterial and/or viral and/or pest pathogens by foliar or root application or by injection. The composition contains at least one active compound, alone, or a number of associated active compounds, selected among the following compounds or families of compounds: trigonelline, 4-hydroxy-isoleucine, saponins, steroidal and triterpenoid sapogenins, and nicotinic acid.

Description

COMPOSITION PHYTOSANITAIRE UTILISEE EN TANT QU'ELICITEUR DES DEFENSES NATURELLES DES PLANTESPHYTOSANITARY COMPOSITION AS AN ELEVATOR OF NATURAL DEFENSE OF PLANTS

La présente invention a pour objet une composition phytosanitaire utilisée en tant qu' éliciteur des défenses naturelles des plantes contre les agents pathogènes fongiques , et/ou bactériens , et/ou viraux, et/ou ravageurs , par application foliaire, racinaire ou par injection .The subject of the present invention is a phytosanitary composition used as elicitor of the natural defenses of plants against fungal and / or bacterial and / or viral and / or pest pathogenic agents by foliar, root or injection application.

Le fait de rechercher des solutions pour combattre les nombreuses pressions parasitaires que les plantes subissent en permanence de la part de nombreux agents pathogènes tels des virus , des bactéries , des champignons ou des insectes , constitue une activité de première importance depuis que l'homme pratique 1' agriculture .The search for solutions to combat the many parasite pressures that plants constantly suffer from many pathogens such as viruses, bacteria, fungi or insects, is an activity of prime importance since the practical man Agriculture.

Ainsi , l' on sait par exemple , que la mise au point de solutions basées sur l' utilisation de produits agrochimiques a permis d' améliorer progressivement la qualité des plantes cultivées et d' augmenter notablement les rendements .Thus, for example, it is known that the development of solutions based on the use of agrochemicals has made it possible to progressively improve the quality of cultivated plants and to considerably increase yields.

Cependant, l' on sait aussi aujourd' hui , que malgré ces avantages indéniables , l' emploi de tels produits présente de nombreuses limitations , dues principalement à leur caractère agressif, tant pour l' agriculteur au moment de l' application, que pour 1' environnement.However, it is also known today that despite these undeniable advantages, the use of such products has many limitations, mainly due to their aggressive nature, both for the farmer at the time of application, environment.

De nombreux chercheurs travaillent par conséquent activement à la recherche d' autres solutions qui ne présentent pas de tels caractères nocifs vis à vis de l' environnement.Many researchers are therefore actively working on finding alternative solutions that do not have such harmful environmental characteristics.

Dans ce cadre là, des études portant sur les mécanismes de défense des plantes ont permis de mettre en évidence que la capacité d'une plante à résister à un agent pathogène résultait soit de la reconnaissance de cet agent pathogène par la plante, au travers d'une relation « gène pour gène », soit de la stimulation de ses défenses contre les parasites sous l' effet des blessures causées par le parasite .In this context, studies on the defense mechanisms of plants have made it possible to demonstrate that the ability of a plant to resist a pathogen resulted either from the recognition of this pathogen by the plant, through 'gene-for-gene' relationship, that is, stimulation of its defenses against parasites as a result of injuries caused by the parasite.

Dans ce dernier cas , l' on s' est aperçu que les mécanismes de défense sont stimulés par certaines substances endogènes , sécrétées par la plante et/ou l' agent pathogène .In the latter case, it has been found that the defense mechanisms are stimulated by certain endogenous substances, secreted by the plant and / or the pathogen.

De telles substances sont par exemple définies , lors d' attaques fongiques , par des oligosaccharides émanant d'une part de la dégradation des parois cellulaires du champignon sous l' action des hydrolases de la plante hôte, et d' autre part, des parois des cellules de la plante hôte , elles-mêmes hydrolysées par les enzymes du champignon . Ces débris de parois servent également de messagers de la résistance .Such substances are, for example, defined, during fungal attacks, by oligosaccharides emanating, on the one hand, from the degradation of the cell walls of the fungus by the hydrolases of the host plant, and on the other hand by the walls of the fungi. cells of the host plant, themselves hydrolysed by the enzymes of the fungus. These wall debris also serve as messengers of the resistance.

Ainsi , en présence de telles substances , la plante développe toute une série de réactions cellulaires , avec notamment la production de molécules destinées à renforcer la résistance des parois cellulaires , mais aussi d' antibiotiques végétaux tels que des phytoalexines ou des protéines de défense . Ces molécules ont des propriétés antifongiques et antibactériennes .Thus, in the presence of such substances, the plant develops a series of cellular reactions, including the production of molecules to enhance the strength of cell walls, but also plant antibiotics such as phytoalexins or defense proteins. These molecules have antifungal and antibacterial properties.

Il a en outre été démontré que suite à un premier déclenchement des mécanismes de résistance , une plante pouvait développer une résistance accrue face à de nouvelles attaques de parasites , notamment au niveau de zones non encore infectées , et ceci à nouveau grâce à certains «messagers chimiques naturels» . Ce mécanisme est appelé «Résistance Systémique Acquise» .It has also been shown that following a first outbreak of the resistance mechanisms, a plant could develop an increased resistance to new parasite attacks, especially in areas not yet infected, and this again thanks to certain "messengers" natural chemicals ". This mechanism is called "Acquired Systemic Resistance".

Ces études ont également permis de constater que lorsqu' une plante est sensible à un agent pathogène (absence de relation « gène pour gène ») , les mécanismes de défense précédemment évoqués ne se déclenchent pas . Une telle plante est également incapable soit de reconnaître son agresseur au travers d'un effet «blessure», soit de réagir suffisamment rapidement si elle le reconnaît grâce aux blessures . II existe actuellement des techniques permettant de stimuler de manière artificielle les défenses naturelles des végétaux, en leur fournissant, de façon exogène, les «signaux chimiques naturels» de la résistance qu' elles sont incapables de produire, dans certains cas , vis à vis de certains agents pathogènes .These studies have also shown that when a plant is sensitive to a pathogen (lack of "gene for gene" relationship), the previously mentioned defense mechanisms do not trigger. Such a plant is also unable to either recognize its aggressor through a "wound" effect, or to react quickly enough if it recognizes it through injury. There are currently techniques for artificially stimulating the natural defenses of plants, by providing, exogenously, the "natural chemical signals" of resistance that they are unable to produce, in some cases, with respect to certain pathogens.

On connaît ainsi plusieurs solutions permettant de stimuler les défenses naturelles des plantes , qui reposent sur l' application auxdites plantes d' un agent éliciteur ou sensibilisateur, capable au contact des plantes de provoquer préventivement dans celles-ci l' activation des gènes de défense sur le site d' application du produit ainsi qu'une résistance systémique acquise .Several solutions are thus known for stimulating the natural defenses of the plants, which are based on the application to said plants of an elicitor or sensitizing agent, capable, in contact with the plants, of preventively causing them to activate the defense genes on them. the site of application of the product as well as acquired systemic resistance.

La demande de brevet français No . 2 766 059 décrit notamment un procédé prévoyant d' utiliser une quantité efficace d' un oligo β 1-3 (1-6) glucane . Celui-ci est préparé à partir de β 1-3- glucanes d' origine diverses qui sont hydrolyses en oligo β 1-3- glucanes . Les β 1-3-glucanes proviennent de bactéries , notamment d'Alcaligenes faecalis , de champignons , notamment de Schizophyllum et de Sderotium glucanium, de levures , notamment de Saccharomyces cerevisae, d' algues ou de céréales .French patent application No. No. 2,766,059 describes in particular a process which provides for the use of an effective amount of a β 1-3 (1-6) glucan oligo. This is prepared from β 1-3 glucans of various origin which are hydrolysed to oligo β 1-3 glucans. The β 1-3 glucans come from bacteria, especially Alcaligenes faecalis, fungi, especially Schizophyllum and Sderotium glucanium, yeasts, in particular Saccharomyces cerevisae, algae or cereals.

En déclenchant un signal d' agression dans la plante, cette molécule empêcherait le développement de nombreux champignons , virus et bactéries et permettraient ainsi aux tomates , blé, orge , riz , haricot et vignes de mieux se défendre contre leurs agresseurs , en réduisant significativement les quantités de fongicides nécessaires .By triggering a signal of aggression in the plant, this molecule prevents the development of many fungi, viruses and bacteria and thus allow tomatoes, wheat, barley, rice, beans and vines to better defend themselves against their aggressors, significantly reducing quantities of fungicides needed.

Cependant, ce procédé n' autorise qu'une seule pulvérisation sur un même végétal , et ne peut donc être mis en oeuvre que de manière limitée dans le temps et essentiellement à titre préventif . L' on connaît encore un procédé de lutte contre les insectes parasites des plantes du brevet US 5 977 060 qui cite de nombreuses références d' éliciteurs .However, this method allows only one spray on the same plant, and can therefore be implemented only in a limited manner over time and essentially as a preventive measure. A method of controlling plant parasitic insects is still known from US Pat. No. 5,977,060, which quotes numerous references from elicitors.

Le procédé décrit dans ce document consiste à appliquer un éliciteur, défini par un polypeptide ou une protéine , à une plante ou à une graine dans des conditions efficaces pour lutter contre les insectes .The method described herein is to apply an elicitor, defined by a polypeptide or protein, to a plant or seed under conditions effective for controlling insects.

L' éliciteur préconisé est obtenu à partir d' un agent pathogène choisi dans le groupe constitué par Erwinia, Pseudomonas , Xanthomonas , Phyrophtora et leurs mélanges .The preferred elicitor is obtained from a pathogen selected from the group consisting of Erwinia, Pseudomonas, Xanthomonas, Phyrophtora and mixtures thereof.

Selon une variante du procédé , l' éliciteur est appliqué aux plantes au travers d'une bactérie contenant des gènes capables de sécréter ou d' exporter le polypeptide ou la protéine .According to a variant of the method, the elicitor is applied to the plants through a bacterium containing genes capable of secreting or exporting the polypeptide or the protein.

Ce document décrit par conséquent un procédé destiné à permettre une lutte spécifiquement dirigée contre les insectes et non contre les agents pathogènes dans leur ensemble . Par ailleurs , le procédé présente l' inconvénient d' intégrer des manipulations génétiques qui sont généralement très mal perçues par le public .This document therefore describes a method for allowing a specific control directed against insects and not against the pathogens as a whole. Moreover, the method has the disadvantage of integrating genetic manipulations which are generally very poorly perceived by the public.

Par ailleurs , le document FR 2 832 409 décrit l'utilisation, en tant qu' éliciteurs des défenses naturelles des végétaux, des oligopeptides cycliques ou hélicoïdaux, obtenus par voie de synthèse organique ou enzymatique , et destinés à être appliqués au niveau des feuilles , des racines ou par injection .Moreover, the document FR 2 832 409 describes the use, as elicitors of the natural defenses of plants, of cyclic or helical oligopeptides, obtained by organic or enzymatic synthesis, and intended to be applied at leaf level, roots or by injection.

La présente demande concerne une nouvelle invention dans le domaine de la stimulation des défenses naturelles des plantes , à savoir une composition phytosanitaire utilisée en tant qu' éliciteur des défenses naturelles des plantes contre les agents pathogènes fongiques , et/ou bactériens , et/ou viraux, et/ou ravageurs , par application foliaire , racinaire ou par injection, caractérisée en ce qu' elle renferme au moins un composé seul ou plusieurs composés en association, choisi (s) parmi les composés ou familles de composés suivants : la trigonelline , la 4-hydroxy-isoleucine, les saponines , les sapogénines stéroïdiques et triterpéniques , l' acide nicotinique .The present application relates to a new invention in the field of stimulating the natural defenses of plants, namely a phytosanitary composition used as elicitor of the natural defenses of plants against fungal pathogens, and / or bacterial, and / or viral and / or pests, by foliar, root or injection application, characterized in that it contains at least one compound alone or in combination with compounds selected from the following compounds or families: trigonelline, 4-hydroxy-isoleucine, saponins, steroidal and triterpenic sapogenins, nicotinic acid.

Des résultats expérimentaux ont permis de montrer qu' une telle composition était particulièrement efficace pour stimuler les défenses des plantes , et qu'un effet de synergie de certains desdits composés en association les une avec les autres pouvait être observé .Experimental results have shown that such a composition was particularly effective in stimulating plant defenses, and that a synergistic effect of some of said compounds in combination with each other could be observed.

Selon une caractéristique avantageuse de l' invention, lesdits composés sont définis par des molécules obtenues à partir d' extraits naturels de plantes , notamment de graines de fenugrec, de café , d' avoine, de verveine , de soja, de fragon, d' orthosyphon.According to an advantageous characteristic of the invention, said compounds are defined by molecules obtained from natural extracts of plants, in particular fenugreek seeds, coffee, oats, verbena, soya beans, borage seeds, orthosyphon.

Cette caractéristique permet à la composition selon l' invention d' être avantageusement en adéquation avec les préoccupations actuelles en matière de protection de l' environnement, lesdits extraits étant entièrement biodégradables et nullement nocifs vis à vis de l' écosystème .This feature allows the composition according to the invention to be advantageously in line with the current concerns in the field of environmental protection, said extracts being entirely biodegradable and not harmful to the ecosystem.

Cependant, l' invention prévoit encore une autre caractéristique de ladite composition selon laquelle lesdits composés sont définis par des molécules obtenues par voie de synthèse chimique .However, the invention provides yet another feature of said composition according to which said compounds are defined by molecules obtained by chemical synthesis.

Ceci est particulièrement intéressant pour les composés difficiles à obtenir par le biais d' extraits naturels .This is particularly interesting for compounds difficult to obtain through natural extracts.

Par ailleurs , selon une variante de l' invention, le composé de la famille des sapogénines est défini par de la diosgénine, extraite des graines de fenugrec .Furthermore, according to a variant of the invention, the sapogenin family compound is defined by diosgenin, extracted from fenugreek seeds.

Une autre particularité de la présente composition est encore définie par le fait qu' elle renferme également des protéines extraites de graines de fenugrec .Another particularity of the present composition is still defined by the fact that it also contains proteins extracted from fenugreek seeds.

Selon une caractéristique additionnelle , la présente composition renferme également un agent fongicide , et/ou un agent bactéricide, et/ou un agent insecticide, et/ou des éléments nutritifs pour les plantes .According to an additional characteristic, the present composition also contains a fungicidal agent, and / or a bactericidal agent, and / or an insecticidal agent, and / or nutrients for the plants.

Plusieurs types de formulation peuvent en outre être avantageusement envisagés .Several types of formulation may further be advantageously contemplated.

Ainsi , la composition selon l' invention peut se présenter sous forme solide , par exemple sous forme de poudre, ou granules , renfermant le ou les composés actifs , seul (s) ou en combinaison avec d' autres agents de formulation .Thus, the composition according to the invention can be in solid form, for example in powder form, or granules, containing the active compound (s), alone or in combination with other formulating agents.

De même , la présente composition peut se présenter sous une forme liquide, auquel cas le ou les composés actifs peuvent être combinés avec des agents mouillants , dispersants et/ou adhésifs .Likewise, the present composition may be in liquid form, in which case the active compound (s) may be combined with wetting, dispersing and / or adhesive agents.

Quelques résultats expérimentaux permettront à présent de mieux comprendre les avantages et les propriétés de la composition phytosanitaire selon la présente invention .Some experimental results will now make it possible to better understand the advantages and the properties of the phytosanitary composition according to the present invention.

Exemple : - Traitement de plantes maraîchères et ornementales par une composition selon l' invention .Example: Treatment of vegetable and ornamental plants with a composition according to the invention.

On pulvérise la composition sur les différentes plantes testées , et on vérifie leur résistance à divers parasites .The composition is sprayed on the various plants tested, and their resistance to various parasites is verified.

A titre d' exemple , on a obtenu des effets très significatifs lors de traitements préventifs et curatifs sur :By way of example, very significant effects have been obtained during preventive and curative treatments on:

- des melons atteints d' oïdium,- melons with powdery mildew,

- des melons atteints de bactériose , - des melons attaqués par des thrips- melons suffering from bacteriosis, - melons attacked by thrips

- des melons attaqués par des aleurodes - des pieds de vigne atteints d' oïdium- melons attacked by whiteflies - vines with powdery mildew

- des pieds de vigne atteints de mildiou .- vines with mildew.

Après comparaison entre les plantes traitées et des plantes non traitées , l' on constate que :After comparison between treated plants and untreated plants, we find that:

1) dans le cas de l' application d' un traitement préventif, les végétaux traités sont peu ou pas attaqués par les parasites1) in the case of the application of a preventive treatment, the treated plants are little or not attacked by the parasites

(contrairement aux végétaux non-traités) , à condition de renouveler le traitement régulièrement.(unlike untreated plants), provided that the treatment is repeated regularly.

2) dans le cas de traitements curatifs , les végétaux traités voient les symptômes des infections diminuer au bout de quelques jours , après la première application de la composition selon 1' invention .2) in the case of curative treatments, the treated plants see the symptoms of infections decrease after a few days, after the first application of the composition according to the invention.

D' autre part, les différents essais réalisés ont également permis de montrer que le traitement peut s' effectuer aussi bien sur de jeunes plantes , que sur des boutures , ou sur des plantes adultes , en fait quel que soit le stade végétatif des plantes , sans nuire à la santé du végétal .On the other hand, the various tests carried out also made it possible to show that the treatment can be carried out as well on young plants, as on cuttings, or on adult plants, in fact whatever the vegetative stage of the plants, without harming the health of the plant.

Il a également été constaté un effet anti-insecte du traitement à l' aide de la composition selon l' invention . It has also been found an anti-insect effect of the treatment with the aid of the composition according to the invention.

Claims

REVENDICATIONS 1. Composition phytosanitaire utilisée en tant qu' éliciteur des défenses naturelles des plantes contre les agents pathogènes fongiques , et/ou bactériens , et/ou viraux, et/ou ravageurs , par application foliaire, racinaire ou par injection, caractérisée en ce qu' elle renferme au moins un composé actif seul ou plusieurs composés actifs en association, choisi (s)parmi les composés ou familles de composés suivants : la trigonelline , la 4-hydroxy-isoleucine, les saponines , les sapogénines stéroïdiques et triterpéniques , l' acide nicotinique .1. Phytosanitary composition used as elicitor of the natural defenses of plants against fungal and / or bacterial and / or viral and / or pest pathogenic agents, by foliar, root or injection application, characterized in that it contains at least one active compound alone or several active compounds in combination, chosen from the following compounds or families of compounds: trigonelline, 4-hydroxy-isoleucine, saponins, steroidal and triterpenic sapogenins, acid nicotine. 2. Composition selon la revendication 1 , caractérisée en ce que le ou lesdits composés actifs sont définis par des molécules obtenues à partir d' extraits naturels de plantes , notamment de graines de fenugrec, de café , d' avoine, de verveine , de soja, de fragon, d' orthosyphon .2. Composition according to claim 1, characterized in that the active compound or compounds are defined by molecules obtained from natural extracts of plants, in particular fenugreek seeds, coffee, oats, verbena, soya beans. , frog, orthosyphon. 3. Composition selon la revendication 1 , caractérisée en ce que le ou lesdits composés actifs sont définis par des molécules obtenues par voie de synthèse chimique .3. Composition according to claim 1, characterized in that the active compound (s) are defined by molecules obtained by chemical synthesis. 4. Composition selon l'une quelconque des revendications 1 ou 2 , caractérisée en ce que le composé actif choisi dans la famille des sapogénines est défini par de la diosgénine , extraite des graines de fenugrec .4. Composition according to any one of claims 1 or 2, characterized in that the active compound selected from the family of sapogenins is defined by diosgenin, extracted from fenugreek seeds. 5. Composition selon l'une quelconque des revendications précédentes , caractérisée en ce qu' elle renferme des protéines extraites de graines de fenugrec .5. Composition according to any one of the preceding claims, characterized in that it contains proteins extracted from fenugreek seeds. 6. Composition selon l'une quelconque des revendications précédentes , caractérisée en ce qu' elle renferme en outre un agent fongicide , et/ou un agent bactéricide, et/ou un agent insecticide , et/ou des éléments nutritifs pour les plantes .6. Composition according to any one of the preceding claims, characterized in that it additionally contains a fungicidal agent, and / or a bactericidal agent, and / or an agent insecticide, and / or nutrients for plants. 7. Composition selon l'une quelconque des revendications précédentes , caractérisée en ce qu' elle se présente sous une forme solide , par exemple sous forme de poudre , ou granules , renfermant le ou les composés actifs , seul (s) ou en combinaison avec d' autres agents de formulation .7. Composition according to any one of the preceding claims, characterized in that it is in a solid form, for example in the form of a powder, or granules, containing the active compound (s), alone or in combination with other formulators. 8. Composition selon l' une quelconque des revendications 1 à 6 , caractérisée en ce qu' elle se présente sous une forme liquide, le ou les composés actifs pouvant être combinés avec des agents mouillants , dispersants et/ou adhésifs . 8. Composition according to any one of claims 1 to 6, characterized in that it is in a liquid form, the active compound (s) can be combined with wetting agents, dispersants and / or adhesives.
PCT/FR2005/050480 2004-06-25 2005-06-22 Phytosanitary composition used as an elicitor of natural defenses of plants Ceased WO2006084964A1 (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008107909A1 (en) 2007-03-05 2008-09-12 Indus Biotech Private Limited Pharmaceutical compositions comprising trigonelline and 4-hydroxyisoleucine and a process thereof
CN104904491A (en) * 2014-03-10 2015-09-16 廉美兰 Method for improving saponin content in American ginseng adventive roots cultured in reactor with ginseng pathogenic bacterium elicitor
CN116172004A (en) * 2023-03-01 2023-05-30 云南农业大学 Niacin-induced expression of plant defense-related genes and use of disease resistance

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK200600154U3 (en) * 2006-05-29 2007-09-14 Nor Natur Aps Natural remedy for use in plant production with effect on specific fungal diseases and production
DK200600155U3 (en) * 2006-05-29 2007-09-14 Nor Natur Aps Natural remedy based on saponin-containing plants such as buckthorn, tea and rosemary for use in plant production
FR3040124A1 (en) * 2015-08-21 2017-02-24 Soc Occitane De Fabrications Et De Tech (S O F T ) COMPOSITION COMPRISING A FENUGRC PURE GERM-COTYLEDON FRACTION AND PHYTOSANITARY APPLICATION

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002102162A1 (en) * 2001-06-15 2002-12-27 Valoragri S.A. Elicitor made from trigonella foenum graecum seed extracts and use thereof in controlling pathogenic organisms of plants
CN1397545A (en) * 2002-08-14 2003-02-19 鹰翰国际贸易(上海)有限公司 A method for extracting various by-products such as 4-hydroxy-isoleucine and fenugreek gum from fenugreek seeds
WO2004107864A1 (en) * 2003-06-10 2004-12-16 S.O.F.T Sarl Elicitor made from trigonella foenum graecum extracts for the treatment of plant pathogens, use thereof, and method for the production thereof
WO2005084323A2 (en) * 2004-03-02 2005-09-15 Technical Sourcing International, Inc. Compositions of bio-active compounds from fenugreek seed and methods for producing same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002102162A1 (en) * 2001-06-15 2002-12-27 Valoragri S.A. Elicitor made from trigonella foenum graecum seed extracts and use thereof in controlling pathogenic organisms of plants
CN1397545A (en) * 2002-08-14 2003-02-19 鹰翰国际贸易(上海)有限公司 A method for extracting various by-products such as 4-hydroxy-isoleucine and fenugreek gum from fenugreek seeds
WO2004107864A1 (en) * 2003-06-10 2004-12-16 S.O.F.T Sarl Elicitor made from trigonella foenum graecum extracts for the treatment of plant pathogens, use thereof, and method for the production thereof
WO2005084323A2 (en) * 2004-03-02 2005-09-15 Technical Sourcing International, Inc. Compositions of bio-active compounds from fenugreek seed and methods for producing same

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BULLETIN OF THE ENTOMOLOGICAL SOCIETY OF EGYPT ECONOMIC SERIES, no. 14, 1984, pages 243 - 250, ISSN: 0081-0991 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1984, BARAKAT A A ET AL: "JOINT ACTION OF SOME PESTICIDES AND PLANT EXTRACTS AGAINST TETRANYCHUS-URTICAE KOCH", XP002321882, Database accession no. PREV198885056595 *
DATABASE WPI Section Ch Week 200365, Derwent World Patents Index; AN 2003-680416, XP002321883, "process for extracting 4-hydroxy isoleucine and by-products includung feungreek gum from seed of feungreek" *
KRASKA, T. AND SCHOENBECK F.: "About Changes in the Chromatin Structure after Resistance Induction in Hordeum vulgare L.", J. PHYTOPATHOLOGA, vol. 137, 1993, BERLIN, HAMBURG, pages 10 - 14, XP001205834 *
OMOLOSO A D ET AL: "BROAD SPECTRUM ANTIBACTERIAL ACTIVITY OF ALLIUM CEPA, ALLIUM ROSEUM, TRIGONELLA FOENUM GRAECUM AND CURCUMA DOMESTICA", NATURAL PRODUCT SCIENCES, KOREAN SOCIETY OF PHARMACOGNOSY, SEOUL, KR, vol. 7, no. 1, 2001, pages 13 - 16, XP001179533, ISSN: 1226-3907 *
PEMONGE, J. ET AL.: "Effects of Materials and Extracts of Trigonella foenum graecum against the Stored Product Pest Tribolium castaneum (Herbst) ..."", J.STORED PROD.RES., vol. 33, no. 3, 1997, GREAT BRITAIN, pages 209 - 217, XP002321880 *
TAYLOR W.G ET AL.: "Analysis of Steroidal Sapogenins from Amber Fungreek (Trigonella foenum-graecum) by Capyllary GC and Combined GC/MS", J.AGRIC.FOOD CHEM., vol. 45, 1997, ACS, pages 753 - 759, XP002321881 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008107909A1 (en) 2007-03-05 2008-09-12 Indus Biotech Private Limited Pharmaceutical compositions comprising trigonelline and 4-hydroxyisoleucine and a process thereof
JP2010523475A (en) * 2007-03-05 2010-07-15 インダス バイオテック プライベート リミテッド Pharmaceutical composition and method for its preparation
KR101145395B1 (en) 2007-03-05 2012-07-10 인두스 바이오텍 프라이빗 리미티드 Pharmaceutical compositions comprising trigonelline and 4-hydroxyisoleucine and a process thereof
CN104904491A (en) * 2014-03-10 2015-09-16 廉美兰 Method for improving saponin content in American ginseng adventive roots cultured in reactor with ginseng pathogenic bacterium elicitor
CN116172004A (en) * 2023-03-01 2023-05-30 云南农业大学 Niacin-induced expression of plant defense-related genes and use of disease resistance

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