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WO2006080019A1 - Nourriture pour abeilles, miel produit par des abeilles ayant consomme cette nourriture et utilisations de ce miel - Google Patents

Nourriture pour abeilles, miel produit par des abeilles ayant consomme cette nourriture et utilisations de ce miel Download PDF

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Publication number
WO2006080019A1
WO2006080019A1 PCT/IL2006/000117 IL2006000117W WO2006080019A1 WO 2006080019 A1 WO2006080019 A1 WO 2006080019A1 IL 2006000117 W IL2006000117 W IL 2006000117W WO 2006080019 A1 WO2006080019 A1 WO 2006080019A1
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WO
WIPO (PCT)
Prior art keywords
honey
blood
food
globin
bee
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IL2006/000117
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English (en)
Inventor
Alexander Goroshit
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
TZUF GLOBUS Ltd
Original Assignee
TZUF GLOBUS Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by TZUF GLOBUS Ltd filed Critical TZUF GLOBUS Ltd
Publication of WO2006080019A1 publication Critical patent/WO2006080019A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/63—Arthropods
    • A61K35/64—Insects, e.g. bees, wasps or fleas
    • A61K35/644—Beeswax; Propolis; Royal jelly; Honey
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/24—Animal feeding-stuffs from material of animal origin from blood
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
    • A23L21/25—Honey; Honey substitutes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/185—Vegetable proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid

Definitions

  • This invention relates to a method for preparing food for bees, the food obtained thereby, honey produced by bees fed with such food, a method for preparing such honey and medicinal uses of such honey.
  • Bee honey is the substance made and stored in the honeycombs of hives by honey bees.
  • the composition of honey is influenced by the type of food collected by the bees. In general, bee food may be divided into two types:
  • Honey products are substances consisting in whole or in part of honey, but which do not meet the compositional criteria for honey.
  • Myelosupression (bone marrow suppression) is the most serious toxic effect of the majority of chemotherapeutic agents and typically is the dose- limiting factor. Death occurring after chemotherapy usually results either from infection related to drug-induced leucopenia or from bleeding related to thrombocytopenia. There is a need in the art for novel means to prevent or ameliorate such side effects of chemotherapy.
  • BE 1,005,748 describes production of honey compositions containing medicinal or herbal products by mixing of foreign substances with bees feed material, e.g. artificial nectar or honey, which can be easily and quickly taken-up in blood stream.
  • CN 1,067,779 describes a method to produce biofunctional honey by feeding bees with Chinese herbal medicines.
  • DE 4,439,732 describes biological composition of animal origin, used as feed additive for mammals, poultry and bees to improve health and productivity.
  • US 5,604,205 describes feed additive for animals, poultry and bees, containing free and bound amino acids and other nutrients, produced by hydrolysis of animal tissue or blood.
  • the first aspect of the invention is a method for preparing food for bees.
  • the method comprises mixing bee food-base with blood, preferably together with one or more plant extracts, to obtain food for bees.
  • the blood is preferably of an avian or a mammal, such as hens, turkeys, cows, sheep and pigs.
  • blood denotes whole blood as well as processed blood.
  • Processed blood typically includes blood which has been homogenized or lysed so as to break up cell membranes and release cellular constituents therefrom. Additional treatments of the blood (i.e. in addition to the breakup of the cell) may be performed, including drying, freezing and thawing, filtrating etc., or any combination of same.
  • Blood may be obtained from avian (e.g. bird, chicken, chick, turkey etc.) as well as from mammalian sources (e.g. cattle).
  • Human blood such as blood that passed its expiration date, obtainable from blood banks, may also be used.
  • the food-base useful according to the invention is any conventional bee food, such as a sugar solution, e.g.
  • the blood is frozen so as to lyse the blood cells, defrosted, and filtered from solid residues to obtain a blood filtrate, which is mixed with the food-base.
  • the defrosting is carried out under vacuum, to obtain dry blood, which may be pulverized and introduced into the bee-food as powder.
  • plant extracts are included in the food for bees.
  • the extracts are plant blanches.
  • suitable plant blanches are those of Echinacea, Uncaria, Eleuther ococcus, Urtica, Calendula, Trifoluim, Melissa, Moms, Ribes,
  • Taraxacum Chicorium, Vaccinium, Avena, Inula, Melilotus, Ficus, and Beta.
  • plant blanches or blanches refers to plant parts which were scalded or boiled in steam or water in order to prepare an extract thereof.
  • the bee-food obtained according to the method of the invetnion preferably has at least 50% food-base, more preferably at least 55%.
  • the bee food has preferably no more than 70% food base, and more preferably no more than 60% food base. All percentages mentioned in the present specification are weight per weight (w/w), unless otherwise stated.
  • the preferred amount of blood or blood filtrate in the bee food is at least 5%, preferably at least 20%.
  • the maximal preferred amount of blood or blood filtrate is 30%, and amounts of around 25% are most preferable.
  • bee food of the invention includes plant extracts as described below, and water (up to 100%).
  • the bee food product of the invention comprises food-base, blood or blood filtrate, and preferably also one or more plant extracts.
  • Another aspect of the invention is honey obtainable from bees fed with bee food according to the invention.
  • honey is used to include any honey-like beehive product, including those that do not fulfill the nutritional definitions of honey as defined by some food regulatory authorities.
  • Natural honey as defined by the National Honey Board of the Untied States (http://www.nhb.org) will be referred to as “pure honey” or "regular honey”.
  • Honey according to the invention was found to contain as much as 6mg protein per Ig honey, which is up to 10 times more than the protein content of honey produced by bees feed with floral bee food.
  • another aspect of the invention is honey containing at least 0.2% protein, preferably at least 0.4% protein, more preferably about 0.5 to 0.7% protein, most preferably about 0.6% protein.
  • proteins found in honey according to the invention include at least one of hemoglobin, beta-globin, rho-globin, alpha A globin, alpha D globin, Ig gamma chain, apolipoprotein Al, Ig light chain, PIT 64 protein, and preproalbumin. In one embodiment of honey according to the invention all these proteins were found in the honey as collected from the beehive.
  • Another aspect of the invention is a method for producing honey from bees comprising:
  • the invention also provides a method of treating side effects of chemotherapy, the method comprising providing a subject undergoing chemotherapy with an effective amount of honey comprising blood components.
  • blood components refers to compounds that are known in the art to be found in blood or to be obtainable from blood, and not to be found in honey obtained from bees that are fed with conventional floral bee-food.
  • Non-limiting examples of blood components are proteins, as found in honey according to the invention.
  • blood components do not include blood hydrolysates.
  • honey comprising blood components is obtained from bees fed with a food premix containing blood components. More preferably, it is obtained from bees fed with bee food according to the invention.
  • honey comprising blood components and “enriched honey product” are used interchangeably in this specification.
  • subject undergoing chemotherapy includes subjects before, during or after treatment.
  • the subject may be provided with one or more doses of the enriched honey product at any stage before, during and/or after treatment.
  • treating refers to preventing, ameliorating and/or eliminating chemotherapy side effects.
  • Non-limiting examples of chemotherapy side effects that may be treated in accordance with the invention include pancytopenia, myelosuppression (bone marrow suppression), neutropnia, anemia, thrombocytopenia, deterioration of hemoglobin levels and decrease in quality of life.
  • compositions for preventing or ameliorating side effects of chemotherapy, the composition comprising as an active ingredient honey comprising blood components.
  • honey is obtained from bees fed with bee food- product containing blood component, and more preferably the honey is obtained from bees fed with bee food product according to the invention.
  • the present invention provides use of honey comprising blood components in the preparation of a pharmaceutical or nutraceutical composition for treating side effects of chemotherapy.
  • Figs. IA- IB provide 2D gel electrophoresis of honey produced from bees fed with conventional honey (Fig. IA) and enriched honey product produced from bees fed with a bee food product according to the invention (Fig. IB). DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS
  • Food for bees was prepared by mixing pure honey with blanches of Echinacea, Uncaria, Eleutherococcus, Urtica, Calendula, Trifoluim, Melissa, Morus, Ribes, Taraxacum, Chicorium, Vaccinium, Avena, Inula, Melilotus, Ficus, and Beta and with blood filtrate of hens.
  • the blood filtrate was prepared from fresh hen blood, supplied by a local slaughter house, frozen for a week at a temperature of about -25 0 C, defrosted by leaving for two days at room temperature, and filtered to obtain blood filtrate which was substantially free from solid particles larger than 0.1mm. The filtrate was then mixed for 10 min. at 4000cps, to improve its homogeneity.
  • a blanch was prepared from equal amounts of the herbs mentioned above.
  • the blood filtrate and the blanch were mixed with 4 liters of pure (regular) honey.
  • the amount of bee-food obtained this way (total of 8 liters) was found suitable for feeding one beehive for a week. After five weeks feeding with the above bee-food, honey was collected. Feeding and collecting were carried out by means known per se in the art of apiculture.
  • the honey of the invention was found to be effective in decreasing the incidence of severe neutropenia in 44% of the patients and decreasing the incidence of anemia in 64% of the patients.
  • the incidence of thrombocytopenia was also very low (only two patients). Hemoglobin levels improved in most patients.
  • One third of the patients reported an improvement of their quality of life during honey intake. No side effects were noticed following honey intake. The clinical results are summarized in Table I.
  • honey of the invention had a significant effect in treating side effects of chemotherapy.
  • Comparative analysis of protein content in regular and enriched honey products was performed by using 2D gel electrophoresis and LC/MS. Significant quantitative and qualitative differences between protein content was determined.
  • the protein pellets were solubilized in isoelectric running buffer containing 0.5% IPG buffer (carrier ampholyte mixture), 7M urea, 2M Thiourea, 2.0% CHAPS (3-[(3-Cholamidopropyl)- dimethylammonio]-l -propane sulfonate) a trace of bromophenol blue and 2OmM DTT.
  • IPG buffer carrier ampholyte mixture
  • 7M urea carrier ampholyte mixture
  • 2M Thiourea 2.0% CHAPS (3-[(3-Cholamidopropyl)- dimethylammonio]-l -propane sulfonate) a trace of bromophenol blue and 2OmM DTT.
  • 200-300 ⁇ g protein was loaded onto each pH 3-10NL, 11 cm IPG strips (BioRad). Protein loading by re-hydration was performed at 20 0 C for 12 hours at 50V followed by isoelectric focusing steps of 200, 500
  • the second dimension of the electrophoresis was carried out using pre-cast gradient gels of 4-20% polyacrylamide (Criterion, BioRad). Gel images were taken with a Flour-S imager (BioRad) and the images were analyzed with the PDQuest software (BioRad). Spots that were differentially expressed were cut out using the BioRad Spot Cutter.
  • the gel spots were treated with 50% acetonitrile in 10 nM ammonium bicarbonate and re-hydrated with 10% acetonitrile in 10 mM ammonium bicarbonate containing about 0.1 ⁇ g trypsin per sample.
  • the gel pieces were incubated overnight at 37 0 C and the resulting peptides were recovered.
  • the tryptic peptides were resolved by reverse-phase chromatography on 0.1 x 300mm fused silica capillaries (J&W, 100 micrometer ID) home filled with porous R2 10 micron (Applied Biosystems). The peptides were eluted using 80 min linear gradient of 5 to 95% acetonitrile with 0.1% formic acid in water at flow rate of about 0.3 ⁇ l/min. The liquid from the column was electrosprayed into an ion trap mass spectrometer (DecaXP, Finnigan, San Jose, CA). Mass spectrometry was performed in the positive ion mode using repetitively full MS scan followed by collision induced dissociation (CID) of the most dominant ion selected from the first MS scan. The mass spectrometry data was compared to simulated proteolysis and CID of the proteins in the NR-NCBI database using the Pep-Miner software (I. Beer, IBM). All the identifications were manually validated.
  • the protein concentration of the honey according to the invention was detected by the Bradford method [Bradford, M. (1976) Anal. Biochem. 72, 248- 254] and found to be 6 mg/g (0.6%) vs. 0.62 mg/g (0.062%) in regular pure honey.
  • 2D gel electrophoresis revealed significant difference in the protein patterns of the two honey types (Fig. 1). Five unique spots were detected in 2D gel of the enriched honey product and identified by LC/MS analysis (Table II).

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Insects & Arthropods (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nutrition Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Physiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Biomedical Technology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Jellies, Jams, And Syrups (AREA)

Abstract

Procédé de production de miel par des abeilles qui consiste (a) à préparer de la nourriture pour abeilles, (b) à donner cette nourriture à des abeilles et (c) à recueillir le miel produit par les abeilles. Cette nourriture pour abeilles contient une base d'aliment pour abeilles et du sang. La présente invention concerne également une méthode de traitement des effets secondaires de la chimiothérapie, ladite méthode consistant à faire prendre à un sujet subissant une chimiothérapie une quantité efficace de ce miel contenant des composants sanguins.
PCT/IL2006/000117 2005-01-31 2006-01-29 Nourriture pour abeilles, miel produit par des abeilles ayant consomme cette nourriture et utilisations de ce miel Ceased WO2006080019A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US64779505P 2005-01-31 2005-01-31
US60/647,795 2005-01-31
US71374505P 2005-09-06 2005-09-06
US60/713,745 2005-09-06

Publications (1)

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WO2006080019A1 true WO2006080019A1 (fr) 2006-08-03

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2475023C2 (ru) * 2011-04-01 2013-02-20 Федеральное государственное образовательное учреждение высшего профессионального образования "Кубанский государственный аграрный университет" Способ профилактики кишечных заболеваний пчел
WO2017013661A1 (fr) 2015-07-22 2017-01-26 Phytopharma International Ltd. Compositions consommables par les abeilles, procédés d'utilisation de ces compositions pour la production de miel, et miel produit par ces procédés
KR20180103480A (ko) * 2017-03-10 2018-09-19 오승재 꿀벌 사료용 화분 떡 제조방법 및 그 화분 떡
EP3384785A1 (fr) * 2017-04-04 2018-10-10 Marquie-Amadeus Gerster Procédé de production des aliments concentant de miel ou de propolis, des aliments et ruche
WO2019035143A3 (fr) * 2017-08-16 2019-06-13 Muniyal Ayurvedic Research Centre Miel médicamenteux et son procédé de préparation
LT6671B (lt) 2019-02-04 2019-10-25 Juozas Pileckas Medaus su pakeista sudėtimi gamybos būdas, naudojant vaistinių augalų priedus
LT6672B (lt) 2019-02-04 2019-10-25 Juozas Pileckas Medunešių bičių sveikatos palaikymo ir gydymo būdas, naudojant vaistinių augalų priedus
EP3698643A1 (fr) 2019-02-04 2020-08-26 Pileckas, Juozas Procédé de maintien de la santé et de traitement d'abeilles mellifères utilisant des additifs à base d'herbes
KR102271883B1 (ko) * 2019-12-26 2021-06-30 현정호 양봉 먹이용 떡사료 조성물 및 이를 통한 양봉 먹이용 떡사료
IT202000008983A1 (it) 2020-04-24 2021-10-24 Paride Caviggioli Metodo di produzione di prodotti delle api ad alto valore aggiunto
KR20220082583A (ko) * 2020-12-10 2022-06-17 재단법인 경남한방항노화연구원 기능성 벌사료 조성물 및 그 제조 방법

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US5505980A (en) * 1993-08-02 1996-04-09 National Deer Horn Limited Method of manufacturing powdered deer blood
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CN1254561A (zh) * 1998-11-23 2000-05-31 湖北中医学院附属医院 鹅血抗癌制剂

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US5505980A (en) * 1993-08-02 1996-04-09 National Deer Horn Limited Method of manufacturing powdered deer blood
CN1113804A (zh) * 1994-05-28 1995-12-27 王坤 一种抗贫血冲剂及其制备方法
CN1254561A (zh) * 1998-11-23 2000-05-31 湖北中医学院附属医院 鹅血抗癌制剂

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Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2475023C2 (ru) * 2011-04-01 2013-02-20 Федеральное государственное образовательное учреждение высшего профессионального образования "Кубанский государственный аграрный университет" Способ профилактики кишечных заболеваний пчел
WO2017013661A1 (fr) 2015-07-22 2017-01-26 Phytopharma International Ltd. Compositions consommables par les abeilles, procédés d'utilisation de ces compositions pour la production de miel, et miel produit par ces procédés
US10278996B2 (en) 2015-07-22 2019-05-07 Phytopharma International Ltd. Bee-ingestible compositions, methods of using same for producing honey and honey produced thereby
US11426434B2 (en) 2015-07-22 2022-08-30 Phytopharma International Ltd. Bee-ingestible compositions, methods of using same for producing honey and honey produced thereby
KR102000275B1 (ko) 2017-03-10 2019-07-16 오승재 꿀벌 사료용 화분 떡 제조방법 및 그 화분 떡
KR20180103480A (ko) * 2017-03-10 2018-09-19 오승재 꿀벌 사료용 화분 떡 제조방법 및 그 화분 떡
EP3384785A1 (fr) * 2017-04-04 2018-10-10 Marquie-Amadeus Gerster Procédé de production des aliments concentant de miel ou de propolis, des aliments et ruche
WO2019035143A3 (fr) * 2017-08-16 2019-06-13 Muniyal Ayurvedic Research Centre Miel médicamenteux et son procédé de préparation
LT6671B (lt) 2019-02-04 2019-10-25 Juozas Pileckas Medaus su pakeista sudėtimi gamybos būdas, naudojant vaistinių augalų priedus
LT6672B (lt) 2019-02-04 2019-10-25 Juozas Pileckas Medunešių bičių sveikatos palaikymo ir gydymo būdas, naudojant vaistinių augalų priedus
EP3698643A1 (fr) 2019-02-04 2020-08-26 Pileckas, Juozas Procédé de maintien de la santé et de traitement d'abeilles mellifères utilisant des additifs à base d'herbes
KR102271883B1 (ko) * 2019-12-26 2021-06-30 현정호 양봉 먹이용 떡사료 조성물 및 이를 통한 양봉 먹이용 떡사료
IT202000008983A1 (it) 2020-04-24 2021-10-24 Paride Caviggioli Metodo di produzione di prodotti delle api ad alto valore aggiunto
KR20220082583A (ko) * 2020-12-10 2022-06-17 재단법인 경남한방항노화연구원 기능성 벌사료 조성물 및 그 제조 방법
KR102569581B1 (ko) 2020-12-10 2023-08-23 합천군 기능성 벌사료 조성물 및 그 제조 방법

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