[go: up one dir, main page]

WO2006071895A3 - Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences - Google Patents

Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences Download PDF

Info

Publication number
WO2006071895A3
WO2006071895A3 PCT/US2005/047143 US2005047143W WO2006071895A3 WO 2006071895 A3 WO2006071895 A3 WO 2006071895A3 US 2005047143 W US2005047143 W US 2005047143W WO 2006071895 A3 WO2006071895 A3 WO 2006071895A3
Authority
WO
WIPO (PCT)
Prior art keywords
electrodes
dna sequence
recognition
target dna
polynucleotide sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2005/047143
Other languages
French (fr)
Other versions
WO2006071895A2 (en
Inventor
Jean-Christophe P Gabriel
Shripal Gandhi
George Gruner
Charles Steven Joiner Jr
Ron Sosnowski
Alexander Star
Eugene Tu
Christian Valcke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanomix Inc
Original Assignee
Nanomix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/212,026 external-priority patent/US20070178477A1/en
Priority claimed from US11/274,747 external-priority patent/US20070208243A1/en
Application filed by Nanomix Inc filed Critical Nanomix Inc
Priority to EP05855662A priority Critical patent/EP1831670A4/en
Priority to JP2007549560A priority patent/JP2008525822A/en
Publication of WO2006071895A2 publication Critical patent/WO2006071895A2/en
Publication of WO2006071895A3 publication Critical patent/WO2006071895A3/en
Anticipated expiration legal-status Critical
Priority to US14/961,572 priority patent/US20160187276A1/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4146—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS involving nanosized elements, e.g. nanotubes, nanowires
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01L—SEMICONDUCTOR DEVICES NOT COVERED BY CLASS H10
    • H01L2924/00—Indexing scheme for arrangements or methods for connecting or disconnecting semiconductor or solid-state bodies as covered by H01L24/00
    • H01L2924/0001—Technical content checked by a classifier
    • H01L2924/0002—Not covered by any one of groups H01L24/00, H01L24/00 and H01L2224/00

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • Nanotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Electrochemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Mathematical Physics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)

Abstract

A nanotube device is configured as an electronic sensor for a target DNA sequence. A film of nanotubes is deposited over electrodes on a substrate. A solution of single-strand DNA is prepared so as to be complementary to a target DNA sequence. The DNA solution is deposited over the electrodes, dried, and removed from the substrate except in a region between the electrodes. The resulting structure includes strands of the desired DNA sequence in direct contact with nanotubes between opposing electrodes, to form a sensor that is electrically responsive to the presence of target DNA strands. Alternative assay embodiments are described which employ linker groups to attach ssDNA probes to the nanotube sensor device.
PCT/US2005/047143 2004-05-14 2005-12-23 Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences Ceased WO2006071895A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP05855662A EP1831670A4 (en) 2004-12-28 2005-12-23 NANOELECTRONIC DEVICES FOR DNA DETECTION AND RECOGNITION OF POLYNUCLEOTIDE SEQUENCES
JP2007549560A JP2008525822A (en) 2004-12-28 2005-12-23 Nanoelectronic device for DNA detection / recognition of polynucleotide sequences
US14/961,572 US20160187276A1 (en) 2004-05-14 2015-12-07 Electrochemical nanosensors for biomolecule detection

Applications Claiming Priority (16)

Application Number Priority Date Filing Date Title
US63995404P 2004-12-28 2004-12-28
US60/639,954 2004-12-28
US65727505P 2005-02-28 2005-02-28
US60/657,275 2005-02-28
US66887905P 2005-04-05 2005-04-05
US60/668,879 2005-04-05
US11/212,026 US20070178477A1 (en) 2002-01-16 2005-08-24 Nanotube sensor devices for DNA detection
US11/212,026 2005-08-24
US73090505P 2005-10-27 2005-10-27
US60/730,905 2005-10-27
US11/274,747 2005-11-15
US11/274,747 US20070208243A1 (en) 2002-01-16 2005-11-15 Nanoelectronic glucose sensors
US73869405P 2005-11-21 2005-11-21
US60/738,694 2005-11-21
US74883405P 2005-12-09 2005-12-09
US60/748,834 2005-12-09

Publications (2)

Publication Number Publication Date
WO2006071895A2 WO2006071895A2 (en) 2006-07-06
WO2006071895A3 true WO2006071895A3 (en) 2007-02-08

Family

ID=36615493

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/047143 Ceased WO2006071895A2 (en) 2004-05-14 2005-12-23 Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences

Country Status (3)

Country Link
EP (1) EP1831670A4 (en)
JP (1) JP2008525822A (en)
WO (1) WO2006071895A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8993346B2 (en) 2009-08-07 2015-03-31 Nanomix, Inc. Magnetic carbon nanotube based biodetection
US9103775B2 (en) 2002-01-16 2015-08-11 Nanomix, Inc. Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices
US9291613B2 (en) 2002-06-21 2016-03-22 Nanomix, Inc. Sensor having a thin-film inhibition layer

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1781771A2 (en) * 2004-08-24 2007-05-09 Nanomix, Inc. Nanotube sensor devices for dna detection
JP2009532064A (en) * 2006-04-04 2009-09-10 ナノミックス・インコーポレーテッド Nanoelectronic detection of biomolecules using analyte amplification and reporter
ES2289948B1 (en) * 2006-07-19 2008-09-16 Starlab Barcelona, S.L. ELECTROPHYSIOLOGICAL SENSOR.
JP5061342B2 (en) * 2006-09-11 2012-10-31 国立大学法人大阪大学 Carbon nanotube electrode and sensor using the electrode
US8465634B2 (en) * 2007-05-23 2013-06-18 Arizona Board Of Regents Systems and methods for integrated electrochemical and electrical detection
EP2197897A4 (en) * 2007-08-30 2012-08-08 Univ Waterloo AMINO ACID MATCHING-BASED SELF-ASSEMBLY PEPTIDES AND METHODS
WO2010005080A1 (en) * 2008-07-10 2010-01-14 株式会社イデアルスター Gas sensor
WO2010039941A2 (en) * 2008-10-02 2010-04-08 University Of New Haven Bionanosensor detection device
GB0822733D0 (en) * 2008-12-12 2009-01-21 Univ Warwick Nanotube electrochemistry
JP2011220803A (en) * 2010-04-08 2011-11-04 Mitsumi Electric Co Ltd Biosensor including field-effect transistor element
GB2499340B (en) 2010-10-04 2015-10-28 Genapsys Inc Methods for sequencing nucleic acids
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
EP2714935B1 (en) * 2011-05-27 2017-03-15 Genapsys Inc. Systems and methods for genetic and biological analysis
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
WO2013082619A1 (en) 2011-12-01 2013-06-06 Genapsys, Inc. Systems and methods for high efficiency electronic sequencing and detection
WO2014060896A1 (en) * 2012-10-16 2014-04-24 Koninklijke Philips N.V. Integrated circuit with nanowire chemfet-sensors, sensing apparatus, measuring method and manufacturing method
CN102938397B (en) * 2012-12-05 2015-09-09 苏州纳格光电科技有限公司 Electronic device and preparation method thereof
CA2896879C (en) 2013-03-15 2020-09-22 Genapsys, Inc. Systems and methods for biological analysis
FI130841B1 (en) * 2013-05-29 2024-04-18 Csir A field effect transistor and a gas detector including a plurality of field effect transistors
EP3792921A1 (en) 2013-12-11 2021-03-17 Genapsys, Inc. Systems and methods for biological analysis and computation
EP3080294B1 (en) 2013-12-12 2018-06-13 Altratech Limited A capacitive sensor and method of use
WO2015161054A2 (en) 2014-04-18 2015-10-22 Genapsys, Inc. Methods and systems for nucleic acid amplification
US20180133679A1 (en) * 2015-04-15 2018-05-17 Alastair J. Malcolm Methods of manufacturing semiconductor arrays
WO2017033922A1 (en) * 2015-08-24 2017-03-02 国立大学法人 東京大学 Method of detecting target substance using field-effect transistor
EP3403079A4 (en) * 2016-01-14 2019-09-04 Roswell Biotechnologies, Inc MOLECULAR SENSORS AND ASSOCIATED METHODS
CN109790575A (en) 2016-07-20 2019-05-21 吉纳普赛斯股份有限公司 Systems and methods for nucleic acid sequencing
EP3684951A4 (en) 2017-09-21 2021-06-16 Genapsys, Inc. Systems and methods for nucleic acid sequencing
US20190219512A1 (en) * 2018-01-12 2019-07-18 Clifford H. Kern, III Device, procedure and system for detecting bacterial pathogens including methicillin-resistant staphylococcus aureus or clostridium difficile
CN113588605B (en) * 2021-06-18 2024-08-06 山东师范大学 Method for detecting estrogen in multiple environments and application thereof
AU2022297910A1 (en) * 2021-06-23 2024-01-18 Peter Maccallum Cancer Institute Conductometric sensor for detecting a nucleic acid and a method for the detection thereof
CA3229708A1 (en) * 2021-08-16 2023-02-23 Diagmetrics, Inc. Diagnostic platform for testing exhaled breath condensate and universal biosensor
US11845079B2 (en) * 2021-12-08 2023-12-19 International Business Machines Corporation Integrated silicon platform for electronic biosensors

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001044796A1 (en) * 1999-12-15 2001-06-21 Board Of Trustees Of The Leland Stanford Junior University Carbon nanotube devices
US20020117659A1 (en) * 2000-12-11 2002-08-29 Lieber Charles M. Nanosensors
US20020179434A1 (en) * 1998-08-14 2002-12-05 The Board Of Trustees Of The Leland Stanford Junior University Carbon nanotube devices
US20040202603A1 (en) * 1994-12-08 2004-10-14 Hyperion Catalysis International, Inc. Functionalized nanotubes

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003322653A (en) * 2002-05-07 2003-11-14 Toshiba Corp Probe fixed support and probe fixed carrier
JP2006505806A (en) * 2002-11-08 2006-02-16 ナノミックス・インコーポレーテッド Electronic detection of nanotube-based biomolecules
JP2004347532A (en) * 2003-05-23 2004-12-09 Japan Science & Technology Agency Biosensor

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040202603A1 (en) * 1994-12-08 2004-10-14 Hyperion Catalysis International, Inc. Functionalized nanotubes
US20020179434A1 (en) * 1998-08-14 2002-12-05 The Board Of Trustees Of The Leland Stanford Junior University Carbon nanotube devices
WO2001044796A1 (en) * 1999-12-15 2001-06-21 Board Of Trustees Of The Leland Stanford Junior University Carbon nanotube devices
US20020117659A1 (en) * 2000-12-11 2002-08-29 Lieber Charles M. Nanosensors

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CUI ET AL.: "Nanowire Nanosensors for Hihgly Sensitive and Selective Detection of Biological and Chemical Species", SCIENCE, vol. 293, 17 August 2001 (2001-08-17), pages 1289 - 1291, XP002951584 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9103775B2 (en) 2002-01-16 2015-08-11 Nanomix, Inc. Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices
US9291613B2 (en) 2002-06-21 2016-03-22 Nanomix, Inc. Sensor having a thin-film inhibition layer
US8993346B2 (en) 2009-08-07 2015-03-31 Nanomix, Inc. Magnetic carbon nanotube based biodetection

Also Published As

Publication number Publication date
EP1831670A4 (en) 2010-09-15
WO2006071895A2 (en) 2006-07-06
EP1831670A2 (en) 2007-09-12
JP2008525822A (en) 2008-07-17

Similar Documents

Publication Publication Date Title
WO2006071895A3 (en) Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences
WO2006024023A3 (en) Nanotube sensor devices for dna detection
Vermisoglou et al. Human virus detection with graphene-based materials
US9753031B2 (en) Aptamer coated measurement and reference electrodes and methods using same for biomarker detection
Roy et al. Direct electrical measurements on single-molecule genomic DNA using single-walled carbon nanotubes
WO2007084076A8 (en) Biosensor
WO2010059687A3 (en) A semiconductor for measuring biological interactions
WO2009049566A3 (en) Device for production of layer of nanofibres through electrostatic spinning of polymer matrices and collecting electrode for such device
Avelino et al. Nanostructured sensor platform based on organic polymer conjugated to metallic nanoparticle for the impedimetric detection of SARS-CoV-2 at various stages of viral infection
WO2008033419A8 (en) Electrochemical sensor with interdigitated microelectrodes and conducted polymer
WO2007101223A3 (en) Analyte sensors and methods of use
MX373009B (en) SALIVARY BIOSENSORS AND BIOFUEL CELLS.
WO2008106903A3 (en) Device for production of nanofibres and/or nanoparticles from solutions or melts of polymers in electrostatic field
WO2008051183A3 (en) Sensing system
WO2011017077A3 (en) Nanochannel-based sensor system with controlled sensitivity
WO2007146369A3 (en) Ammonia gas sensor with dissimilar electrodes
JP2010512015A5 (en)
EP2112580A3 (en) Coordinate detection apparatus
WO2015054663A3 (en) Biomolecular interaction detection devices and methods
WO2017173042A3 (en) Nanostructure array based sensors for electrochemical sensing, capacitive sensing and field-emission sensing
EP1927851A3 (en) Biosensor
HK1251621A1 (en) Biosensor
WO2007142880A3 (en) Impact sensor
WO2009145836A3 (en) Methods and devices based upon a novel form of nucleic acid duplex on a surface
Mai et al. Highly sensitive DNA sensor based on polypyrrole nanowire

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2005855662

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2007549560

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE