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WO2006055395A3 - Dispositif optique pour mesurer les proprietes optiques d'un echantillon et procede associe - Google Patents

Dispositif optique pour mesurer les proprietes optiques d'un echantillon et procede associe Download PDF

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Publication number
WO2006055395A3
WO2006055395A3 PCT/US2005/040796 US2005040796W WO2006055395A3 WO 2006055395 A3 WO2006055395 A3 WO 2006055395A3 US 2005040796 W US2005040796 W US 2005040796W WO 2006055395 A3 WO2006055395 A3 WO 2006055395A3
Authority
WO
WIPO (PCT)
Prior art keywords
sample
cell
flow
excitation
measuring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2005/040796
Other languages
English (en)
Other versions
WO2006055395A2 (fr
Inventor
Matthew S Hull
Joshua P Averett
Mark E Jones
Daniel R Klemer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Luna Innovations Inc
Original Assignee
Luna Innovations Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Luna Innovations Inc filed Critical Luna Innovations Inc
Publication of WO2006055395A2 publication Critical patent/WO2006055395A2/fr
Publication of WO2006055395A3 publication Critical patent/WO2006055395A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28Investigating the spectrum
    • G01J3/42Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28Investigating the spectrum
    • G01J3/44Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406Fluorescence spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03Cuvette constructions
    • G01N21/05Flow-through cuvettes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02Details
    • G01J3/0205Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/0218Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using optical fibers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N2021/6482Sample cells, cuvettes

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)

Abstract

L'invention concerne un dispositif et un procédé servant à mesurer les propriétés optiques d'un échantillon. Le dispositif comprend un corps entourant une cuve à circulation à flux continu. La cuve à circulation comporte une entrée d'échantillon placée à proximité d'une première extrémité de la cuve, et une sortie d'échantillon placée à proximité d'une seconde extrémité de celle-ci. Une chambre d'échantillon est placée entre l'entrée et la sortie d'échantillon. Une pluralité de sources d'excitation sont mises en place sur le corps de manière à pouvoir agir sur la chambre d'échantillon. Au moins une source d'excitation comporte une longueur d'onde différente des autres sources d'excitation. Chaque source d'excitation agit sur un échantillon de la cuve à circulation. Au moins un détecteur d'émission fluorescente est positionné de façon opérationnelle par rapport à la cuve à circulation. Ledit détecteur détecte un spectre continu à large bande de longueurs d'onde d'émission émises par l'échantillon lorsque celui-ci est soumis à l'action de chaque source d'excitation. Au moins un système d'interrogation de signal, positionné de façon opérationnelle par rapport à chaque détecteur, interprète un spectre continu d'émission fluorescente. Le dispositif comprend aussi au moins un détecteur d'absorbance servant à mesurer la puissance transmise qui dépend, d'un point de vue spectral, des caractéristiques d'absorbance de l'échantillon.
PCT/US2005/040796 2004-11-15 2005-11-14 Dispositif optique pour mesurer les proprietes optiques d'un echantillon et procede associe Ceased WO2006055395A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US62784204P 2004-11-15 2004-11-15
US60/627,842 2004-11-15

Publications (2)

Publication Number Publication Date
WO2006055395A2 WO2006055395A2 (fr) 2006-05-26
WO2006055395A3 true WO2006055395A3 (fr) 2007-03-22

Family

ID=36407644

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/040796 Ceased WO2006055395A2 (fr) 2004-11-15 2005-11-14 Dispositif optique pour mesurer les proprietes optiques d'un echantillon et procede associe

Country Status (1)

Country Link
WO (1) WO2006055395A2 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030058440A1 (en) * 2001-08-28 2003-03-27 Scott Graham B. I. Pulsed-multiline excitation for color-blind fluorescence detection
US6683314B2 (en) * 2001-08-28 2004-01-27 Becton, Dickinson And Company Fluorescence detection instrument with reflective transfer legs for color decimation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030058440A1 (en) * 2001-08-28 2003-03-27 Scott Graham B. I. Pulsed-multiline excitation for color-blind fluorescence detection
US6683314B2 (en) * 2001-08-28 2004-01-27 Becton, Dickinson And Company Fluorescence detection instrument with reflective transfer legs for color decimation

Also Published As

Publication number Publication date
WO2006055395A2 (fr) 2006-05-26

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