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WO2006055371A3 - Functional screening of antibody libraries - Google Patents

Functional screening of antibody libraries Download PDF

Info

Publication number
WO2006055371A3
WO2006055371A3 PCT/US2005/040598 US2005040598W WO2006055371A3 WO 2006055371 A3 WO2006055371 A3 WO 2006055371A3 US 2005040598 W US2005040598 W US 2005040598W WO 2006055371 A3 WO2006055371 A3 WO 2006055371A3
Authority
WO
WIPO (PCT)
Prior art keywords
antibody
antibody libraries
functional screening
target cells
candidates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2005/040598
Other languages
French (fr)
Other versions
WO2006055371A2 (en
WO2006055371A8 (en
Inventor
Toshiaki Maruyama
Daniela Oltean
Ferda Yantiri-Wernimont
Hong Xin
Amara Siva
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alexion Pharmaceuticals Inc
Original Assignee
Alexion Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alexion Pharmaceuticals Inc filed Critical Alexion Pharmaceuticals Inc
Publication of WO2006055371A2 publication Critical patent/WO2006055371A2/en
Publication of WO2006055371A3 publication Critical patent/WO2006055371A3/en
Publication of WO2006055371A8 publication Critical patent/WO2006055371A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in epitope analysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

High throughput screening of antibody candidates to identify those having desired functional effects on target cells is provided by contacting antibody candidates with plated target cells in the presence of a secondary, anti-antibody antibody.
PCT/US2005/040598 2004-11-16 2005-11-08 Functional screening of antibody libraries Ceased WO2006055371A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US62849704P 2004-11-16 2004-11-16
US60/628,497 2004-11-16

Publications (3)

Publication Number Publication Date
WO2006055371A2 WO2006055371A2 (en) 2006-05-26
WO2006055371A3 true WO2006055371A3 (en) 2006-08-17
WO2006055371A8 WO2006055371A8 (en) 2006-12-07

Family

ID=36407630

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/040598 Ceased WO2006055371A2 (en) 2004-11-16 2005-11-08 Functional screening of antibody libraries

Country Status (1)

Country Link
WO (1) WO2006055371A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2076769B1 (en) 2006-10-03 2018-04-25 Genmab A/S High througput methods for characterization of antibodies
CN104356225B (en) * 2007-08-20 2018-02-13 肿瘤疗法科学股份有限公司 CDH3 peptides and the medicament containing CDH3 peptides
TW201008574A (en) 2008-08-19 2010-03-01 Oncotherapy Science Inc INHBB epitope peptides and vaccines containing the same
WO2013143026A1 (en) * 2012-03-31 2013-10-03 Abmart (Shanghai) Co., Ltd Peptide and antibody libraries and uses thereof
CN104379821A (en) * 2012-03-31 2015-02-25 艾比玛特生物医药（上海）有限公司 Peptide and antibody libraries and uses thereof
WO2018133039A1 (en) * 2017-01-20 2018-07-26 深圳市新产业生物医学工程股份有限公司 Method and kit for preparing antibody pair and use of kit, and system for preparing antibody pair
JP2024530527A (en) * 2021-08-13 2024-08-21 アブウィズ バイオ，インコーポレイテッド Methods for protein and antibody humanization, affinity maturation, and optimization

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002048193A2 (en) * 2000-12-13 2002-06-20 Unilever N.V. Camelidae antibody arrays
WO2003068916A2 (en) * 2002-02-11 2003-08-21 Alexion Pharmaceuticals, Inc. Methods and apparatus for whole cell panning

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002048193A2 (en) * 2000-12-13 2002-06-20 Unilever N.V. Camelidae antibody arrays
WO2003068916A2 (en) * 2002-02-11 2003-08-21 Alexion Pharmaceuticals, Inc. Methods and apparatus for whole cell panning

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HOOGENBOOM HENNIE R: "Selecting and screening recombinant antibody libraries.", NATURE BIOTECHNOLOGY. SEP 2005, vol. 23, no. 9, September 2005 (2005-09-01), pages 1105 - 1116, XP002383647, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
WO2006055371A2 (en) 2006-05-26
WO2006055371A8 (en) 2006-12-07

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