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WO2006050275A3 - Procede de detection rapide specifique par espece d'agents pathogenes - Google Patents

Procede de detection rapide specifique par espece d'agents pathogenes Download PDF

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Publication number
WO2006050275A3
WO2006050275A3 PCT/US2005/039292 US2005039292W WO2006050275A3 WO 2006050275 A3 WO2006050275 A3 WO 2006050275A3 US 2005039292 W US2005039292 W US 2005039292W WO 2006050275 A3 WO2006050275 A3 WO 2006050275A3
Authority
WO
WIPO (PCT)
Prior art keywords
pathogen
specimen
lysate
pathogens
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2005/039292
Other languages
English (en)
Other versions
WO2006050275A2 (fr
Inventor
Joseph C Liao
David A Haake
Bernard M Churchill
Marc A Suchard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Publication of WO2006050275A2 publication Critical patent/WO2006050275A2/fr
Publication of WO2006050275A3 publication Critical patent/WO2006050275A3/fr
Priority to US11/743,071 priority Critical patent/US7763426B2/en
Anticipated expiration legal-status Critical
Priority to US12/788,101 priority patent/US20110027782A1/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Nanotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Composite Materials (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Materials Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé destiné à la détection d'un agent pathogène dans un échantillon. Ce procédé consiste à mettre un lysat de cet échantillon en contact avec une sonde de capture immobilisée sur un substrat. Ce lysat peut être préparé rapidement à partir d'un échantillon clinique, ce qui évite le recours à une culture d'échantillons ou à une épuration bactérienne avant l'analyse. La sonde de capture comprend un premier oligonucléotide qui s'hybride spécifiquement avec une première séquence nucléotidique de l'agent pathogène à détecter. Le lysat est mis en contact avec une sonde de détection comprenant un oligonucléotide marqué, décelable, qui s'hybride spécifiquement avec une seconde séquence nucléotidique de l'agent pathogène. La détection du second marqueur décelable combiné avec le substrat indique la présence de l'agent correspondant. L'invention concerne également des variations de ce procédé, permettant d'optimiser la détection. L'invention concerne également des sondes et des trousses destinées à être utilisées dans ce procédé.
PCT/US2005/039292 2004-11-01 2005-11-01 Procede de detection rapide specifique par espece d'agents pathogenes Ceased WO2006050275A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US11/743,071 US7763426B2 (en) 2004-11-01 2007-05-01 Probes and methods for detection of Escheridia coli and antibiotic resistance
US12/788,101 US20110027782A1 (en) 2004-11-01 2010-05-26 Probes and methods for detection of pathogens and antibiotic resistance

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US62390304P 2004-11-01 2004-11-01
US60/623,903 2004-11-01

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/743,071 Continuation-In-Part US7763426B2 (en) 2004-11-01 2007-05-01 Probes and methods for detection of Escheridia coli and antibiotic resistance

Publications (2)

Publication Number Publication Date
WO2006050275A2 WO2006050275A2 (fr) 2006-05-11
WO2006050275A3 true WO2006050275A3 (fr) 2007-04-12

Family

ID=36319743

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/039292 Ceased WO2006050275A2 (fr) 2004-11-01 2005-11-01 Procede de detection rapide specifique par espece d'agents pathogenes

Country Status (1)

Country Link
WO (1) WO2006050275A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103773842A (zh) * 2013-10-18 2014-05-07 南京农业大学 一种用于检测尿路感染病原菌的特异性纳米金dna探针
WO2017088169A1 (fr) * 2015-11-27 2017-06-01 Coyote Bioscience Co., Ltd. Procédés et systèmes d'amplification d'acides nucléiques

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6348314B1 (en) * 1996-01-24 2002-02-19 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6391558B1 (en) * 1997-03-18 2002-05-21 Andcare, Inc. Electrochemical detection of nucleic acid sequences

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6348314B1 (en) * 1996-01-24 2002-02-19 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6391558B1 (en) * 1997-03-18 2002-05-21 Andcare, Inc. Electrochemical detection of nucleic acid sequences

Also Published As

Publication number Publication date
WO2006050275A2 (fr) 2006-05-11

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