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WO2006047299A3 - Organotypic slice cultures and uses thereof - Google Patents

Organotypic slice cultures and uses thereof Download PDF

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Publication number
WO2006047299A3
WO2006047299A3 PCT/US2005/037924 US2005037924W WO2006047299A3 WO 2006047299 A3 WO2006047299 A3 WO 2006047299A3 US 2005037924 W US2005037924 W US 2005037924W WO 2006047299 A3 WO2006047299 A3 WO 2006047299A3
Authority
WO
WIPO (PCT)
Prior art keywords
observed
culture
sections
unique
culture system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2005/037924
Other languages
French (fr)
Other versions
WO2006047299A2 (en
Inventor
Sean M Kearns
Dennis Steindler
Bjorn Scheffler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Florida
University of Florida Research Foundation Inc
Original Assignee
University of Florida
University of Florida Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Florida, University of Florida Research Foundation Inc filed Critical University of Florida
Publication of WO2006047299A2 publication Critical patent/WO2006047299A2/en
Anticipated expiration legal-status Critical
Publication of WO2006047299A3 publication Critical patent/WO2006047299A3/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Neurology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurosurgery (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The slice culture system allows for the long term survival of both mouse and rat sagittal sections. These sections can be kept viable for periods longer than 6 weeks in culture. Slice culture model systems provide a unique opportunity to monitor and lesion brain circuits in a dish. and cells can be tracked in real time. In addition, cell differentiation can be observed within these slices and the effects of morphogenetic proteins, e.g. extracellular matrix molecules, drugs or small molecules can be observed. This unique culture system provides a useful new method for screening cell and molecular therapies for neurodegenerative diseases.
PCT/US2005/037924 2004-10-22 2005-10-21 Organotypic slice cultures and uses thereof Ceased WO2006047299A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US62123704P 2004-10-22 2004-10-22
US60/621,237 2004-10-22

Publications (2)

Publication Number Publication Date
WO2006047299A2 WO2006047299A2 (en) 2006-05-04
WO2006047299A3 true WO2006047299A3 (en) 2009-04-09

Family

ID=36228280

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/037924 Ceased WO2006047299A2 (en) 2004-10-22 2005-10-21 Organotypic slice cultures and uses thereof

Country Status (1)

Country Link
WO (1) WO2006047299A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8198083B1 (en) 2007-10-31 2012-06-12 William Gunter Loudon Organotypic slices of the central nervous system
EP2103933B1 (en) 2008-02-25 2011-07-27 Universität Leipzig Device and method for measuring impedance in organotypic tissues
ES2368054B1 (en) * 2009-06-16 2012-09-13 Universidad Del Pais Vasco ORGANIC MODEL OF NEURO-DEGENERATIVE DISEASES, PROCESS OF OBTAINING AND USES OF THE SAME
EP2708889A1 (en) 2012-09-12 2014-03-19 Universität Leipzig Method and device for obtaining a tissue sample and for characterizing the tissue sample by determining at least one electrical property
WO2016172693A1 (en) * 2015-04-23 2016-10-27 Rowan University Novel slice cultures and methods for diagnosing neuronal degeneration diseases

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5360610A (en) * 1990-05-16 1994-11-01 Southern Research Institute Method for stimulating nerve fiber growth
US20030040518A1 (en) * 2000-01-24 2003-02-27 Mette Gronborg Isatine derivatives with neurotrophic activity

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5360610A (en) * 1990-05-16 1994-11-01 Southern Research Institute Method for stimulating nerve fiber growth
US20030040518A1 (en) * 2000-01-24 2003-02-27 Mette Gronborg Isatine derivatives with neurotrophic activity

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BECQ H. ET AL.: "Anatomical and functional reconstruction of the nigrostriatal system in vitro: Selective innervation of the striatum by dopaminergic neurons", JOURNAL OF NEUROSCIENCE RESEARCH, vol. 58, no. 4, 15 November 1999 (1999-11-15), pages 553 - 566 *
PLENZ D ET AL.: "Up and Down States in Striatal Medium Spiny Neurons Simultaneously Recorded with Spontaneous Activity in Fast-Spiking Interneurons Studied in Cortex-Striatum-Substantia Nigra Organotypic Cultures", JOURNAL OF NEUROSCIENCE, vol. 18, no. 1, 1998, pages 266 - 283 *
POTHIS E. N. ET AL.: "Synaptic Vesicle Transporter Expression Regulates Vesicle Phenotype and Quantal Size", JOURNAL OF NEUROSCIENCE, vol. 20, 2000, pages 7297 - 7306 *
THOMAS M. P. ET AL.: "Survival and functional demonstration of interregional pathways in fore/midbrain slice explant cultures", NEUROSCIENCE, vol. 85, no. 2, April 1998 (1998-04-01), pages 615 - 626 *
THOMAS, JOURNAL OF NEUROSCIENCE METHODS, vol. 84, 1998, pages 181 - 191 *

Also Published As

Publication number Publication date
WO2006047299A2 (en) 2006-05-04

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