[go: up one dir, main page]

WO2005120255A1 - Processus de production d'une substance contenant de la chlorophylle - Google Patents

Processus de production d'une substance contenant de la chlorophylle Download PDF

Info

Publication number
WO2005120255A1
WO2005120255A1 PCT/JP2004/008223 JP2004008223W WO2005120255A1 WO 2005120255 A1 WO2005120255 A1 WO 2005120255A1 JP 2004008223 W JP2004008223 W JP 2004008223W WO 2005120255 A1 WO2005120255 A1 WO 2005120255A1
Authority
WO
WIPO (PCT)
Prior art keywords
chlorophyll
water
containing material
producing
containing substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2004/008223
Other languages
English (en)
Japanese (ja)
Inventor
Susumu Takayama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to PCT/JP2004/008223 priority Critical patent/WO2005120255A1/fr
Publication of WO2005120255A1 publication Critical patent/WO2005120255A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
    • A23L27/11—Natural spices, flavouring agents or condiments; Extracts thereof obtained by solvent extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/63—Arthropods

Definitions

  • the present invention is intended to remove off-flavors from chlorophyll-containing substances extracted from chlorophyll raw materials mainly composed of one or both of silkworm dung and ground silkworm, and preserved while maintaining freshness. And a method for producing a chlorophyll-containing substance.
  • Chlorophyll also called chlorophyll, is a green pigment that is present in chloroplast substrates in green leaf cells of plants, for example, along with carotene, rutin, and xanthophyll. Chlorophyll is used as a health food processed into beverages, powders, or tablets, for example, because it has an action of accelerating the recovery of skin diseases or burns, or an action of preventing bad smell.
  • this chlorophyll is extracted by washing one or both of the leaves and stems of the plant, crushing it into small pieces as necessary using, for example, a mechanical crushing means of a mixer or a juicer, and squeezing the crushed material. It had been. However, because chlorophyll is located in the hard cell membrane of plants, this method did not extract much chlorophyll from plants.
  • the chlorophyll-containing substance is mainly extracted from the feces of the silkworm in the silkworm, and thus contains water-soluble off-flavors including ammonia.
  • An unpleasant odor was generated from the chlorophyll-containing material.
  • chlorophyll-containing substances as, for example, food or cosmetic materials.
  • Japanese Patent Application Laid-Open No. 59-25694 proposes a method of eliminating odors by mixing enzymes with chlorophyll-containing substances.
  • this method requires special addition of enzymes to the chlorophyll-containing material, and furthermore, the chlorophyll-containing material contains the rest of the added enzyme and decomposition products, and as a result, the chlorophyll-containing material is contaminated. Contamination, leaving problems for future use.
  • the amount of the enzyme added is increased, the chlorophyll purity is reduced and the oxidation of the chlorophyll is accelerated, so that there is a problem that the quality of the product is deteriorated and the life is shortened.
  • the present invention has been made in view of a powerful situation, and it is an object of the present invention to provide an odorant contained in a chlorophyll-containing material obtained by extracting chlorophyll from a chlorophyll material mainly composed of one or both of silkworm dung and ground silkworm. It is an object of the present invention to provide a method for producing a chlorophyll-containing substance that can be preserved while maintaining freshness by removing chlorophyll.
  • a method for producing a chlorophyll-containing material according to the present invention according to the present invention comprises extracting chlorophyll from a chlorophyll raw material mainly containing one or both of silkworm dung and ground silkworm using a volatile solvent. Performing a treatment to form a mixed solution, removing the fiber content from the mixed solution, and adding the insufficient water to the chlorophyll-containing material containing the oil and fat from which the solvent has been removed, and mixing the mixture to form a kneaded product; ,
  • the kneaded material is cooled and held at a temperature higher than o ° C and lower than the temperature at which the fat and oil becomes a solid, and the water is allowed to settle, thereby removing the water-soluble off-flavor containing ammonia contained in the chlorophyll-containing material.
  • a second step of dissolving in water is cooled and held at a temperature higher than o ° C and lower than the temperature at which the fat and oil becomes a solid, and the water is allowed to settle, thereby removing the water-soluble off-flavor containing ammonia contained in the chlorophyll-containing material.
  • the crushed silkworm used as a raw material for chlorophyll is a 4th to 5th year silkworm that contains a large amount of mulberry leaf components. It is preferable to use silkworms of silkworms. It is preferable to use freeze-dried (freeze-dried) live alive silkworms and then pulverize them.
  • a chlorophyll raw material mainly composed of one or both of silkworm dung and ground silkworm is one or both of silkworm dung and silkworm ground material in a chlorophyll raw material in an amount of 70% by mass or more, preferably 80% by mass or more, more preferably 90% by mass or more.
  • Other components include, for example, rice husks, mulberry leaf debris, and moulting husks attached to silkworm dung and silkworms.
  • a chlorophyll-containing substance containing an oil or fat generally contains an oil or fat and a protein in addition to chlorophyll.
  • the amount of water added to the chlorophyll-containing material in the kneaded material was such that the chlorophyll-containing material was washed in the second step and the off-flavor contained in the chlorophyll-containing material could be removed. It is determined by whether or not it is included. Therefore, when the amount of water contained in the chlorophyll-containing material extracted from the chlorophyll raw material satisfies the specified amount, the process proceeds to the second step without adding water to the chlorophyll-containing material in the first step. On the other hand, if the amount of water contained in the chlorophyll raw material is less than the specified amount, add insufficient water to the chlorophyll-containing material and proceed to the second step
  • the cooling temperature of the kneaded material in the second step is preferably higher than 0 ° C and lower than 10 ° C.
  • the cooling temperature of the kneaded material when the cooling temperature of the kneaded material is 0 ° C or lower, the water cannot coagulate and dissolve the off-flavor in the water.
  • the temperature when the temperature is 10 ° C. or higher, the fats and oils contained in the chlorophyll-containing material do not become solids, or it takes time until they become solids, and the productivity of the chlorophyll-containing materials deteriorates. Therefore, in order to remove off-flavors from the chlorophyll-containing material and to improve the productivity of the chlorophyll-containing material, the lower limit of the cooling temperature of the kneaded material exceeds o ° c.
  • the temperature is set at 3 ° C.
  • the mixed solution before the completion of the extraction process in the first step (that is, at the time when the solvent and the chlorophyll raw material are mixed, or during the extraction process), and the fiber component is removed in the first step.
  • a small amount of humic soil extraction water having bactericidal and antibacterial properties is added to any one of the mixed liquid before removing the solvent and the chlorophyll-containing material produced in the third step. Good to be.
  • This humus extract may, for example, contain sediments (for example, seagrass, plants, algae, fish and shellfish, and other minerals) that have been deposited at the bottom of seabeds, lakes, or swamps more than 5 million years ago. Or 2 or more) is water extracted from humus, which is subjected to one or more of the decomposition, synthesis, and / or organicization by anaerobic microorganisms and exists as a layer about 1020 m below the ground.
  • This humus soil extraction water may be, for example, (1) humic acid (humic acid), fulvic acid, or humic humus. It contains substances that make up soil, (2) lipolytic enzymes such as lipase, and (3) enzymes such as alcoholytic enzymes, and (4) proteins, amino acids, fatty acids, organic acids, vitamins, minerals, and It has one or more reducing substances.
  • humus extract water When humus extract water is added to the mixed solution before the completion of the extraction treatment in the first step, not only the chlorophyll-containing material but also the fiber in the chlorophyll raw material can be sterilized and subjected to antibacterial treatment.
  • the humus extraction water is added to the mixture before removing the solvent after the removal of the fiber content in the first step, the humus is added to the chlorophyll-containing material by, for example, stirring means at the time of removing the solvent.
  • the chlorophyll-containing material can be sterilized and antibacterial treated while mixing the soil extraction water substantially uniformly.
  • humus extract water is added to the chlorophyll-containing material produced in the third step, for example, the humus soil extract water is roughly added to the chlorophyll-containing material in which the oils and fats have become solid using stirring means.
  • the humic soil extraction water can be almost uniformly mixed with the chlorophyll-containing material liquefied by hot water roasting, and the chlorophyll-containing material can be sterilized and antibacterial treated.
  • the input amount of the humic soil extract water was converted to a stock solution (aqueous solution) containing 4.6 mg / liter of humic acids calculated based on nitrohumic acid, that is, this stock solution was produced in the third step.
  • the weight ratio to the chlorophyll-containing material to be used is preferably in the range of 1/5000 or more and 1/20 or less.
  • the stock solution may be diluted with, for example, 210-fold water and then added.
  • the amount of humus extract water added to the chlorophyll-containing material is less than 1/5000 of the weight of the chlorophyll-containing material, the addition of humus soil extract water to the chlorophyll-containing material The amount may be too small, and the effect of humus extract water may not be obtained.
  • the amount of humus extract water added to the chlorophyll-containing material is more than 1/20 of the weight of the chlorophyll-containing material, the effect of the humus extract water cannot be remarkably improved, and the humus soil extract water cannot be expected.
  • the upper limit of the amount of added syrup of humus extract water is set to 1Z20, preferably 1/100, more preferably 1/20 of the weight of the chlorophyll-containing material.
  • the lower limit is set to 1Z5000, preferably 1/4000, more preferably 1/1000 of the weight of the chlorophyll-containing material.
  • the chlorophyll-containing material produced in the third step is preferably used for cosmetics, foods, food additives, and beverages.
  • the cosmetic is, for example, pomade, soap, or lotion.
  • the fiber content removed from the mixed solution in the first step is added to the chlorophyll-containing material produced in the third step to obtain a chlorophyll-containing powder, a raw material for tablets or a food additive. Is good.
  • the fiber used here is preferably sterilized and antibacterial-treated with humus extraction water in advance.
  • FIG. 1 is an explanatory diagram of a method for producing a chlorophyll-containing material according to one embodiment of the present invention.
  • FIG. 2 is an explanatory view of a method for producing a chlorophyll-containing substance according to one embodiment of the present invention.
  • FIG. 3 is an explanatory diagram of a chlorophyll extraction device used in the method for producing a chlorophyll-containing substance.
  • the method for producing a chlorophyll-containing material mainly comprises one or both of silkworm dung and ground silkworm.
  • Chlorophyll raw material 10 Chlorophyll is extracted using alcohol such as ethanol (an example of a solvent having volatility) 11 from chlorophyll 10 to form a mixed liquid, and the mixed liquid power contains chlorophyll containing fiber and oil 11 from which alcohol 11 has been removed.
  • kneaded material 14 is prepared by adding insufficient water 13 to 12 and mixing to form a kneaded material 14.Kneaded material 14 is cooled to a temperature above 0 ° C and lower than the temperature at which fats and oils become solids.
  • the silkworm dung used as the chlorophyll raw material 10 contains a lot of mulberry leaf cells, which are the feed for the silkworm, and can be extracted by eluting the chlorophyll into the alcohol 11. After drying the silkworm dung and selecting foreign substances, the silkworm dung that has become rice grain is pulverized18.
  • selection of foreign matter The separation can be performed, for example, by using a wind filter or a sieve, but may be performed manually when the amount is small. Further, it is also possible to wash the silkworm dung and sort out foreign substances.
  • chlorophyll is extracted from the chlorophyll raw material 10 into alcohol 11 using, for example, a chlorophyll extractor disclosed in Japanese Patent Application Laid-Open No. 2004-57105.
  • the chlorophyll extraction device 20 shown in FIG. 3 has first and second tanks 23 and 24 having stirring blades 21 and 22, respectively.
  • Liquid outlets 25 and 26 are provided at the lower positions of the first and second tanks 23 and 24, respectively, and liquid inlets 27 and 28 and raw material input are provided at the upper positions of the first and second tanks 23 and 24. Mouths 29 and 30 are provided respectively.
  • the liquid outlet 25 of the first tank 23 and the liquid inlet 28 of the second tank 24 are connected to a first communication branch pipe 32 having a first valve 31, a pump 33 and an ultrasonic pump in the middle. It is connected by a shared pipe 35 having a vibration means 34 and a second communicating branch pipe 37 having a second valve 36.
  • the liquid outlet 26 of the second tank 24 and the night population 27 of the first tank 23 are connected to the third communication branch pipe 39 having the third noreb 38, the common pipe 35, and the fourth valve 40.
  • the chlorophyll extraction device has one tank, stirring blades rotatably provided inside the lower side of the tank, and a lower portion around the inside of the tank facing the flow of the processing liquid stirred by the stirring blades. And a plurality of ultrasonic vibrators provided in an inclined state.
  • the alcoholic 11 is supplied into the first tank 23 (or the second tank 24), for example, at 50 400 litters.
  • 50 to 200 kg of the crushed chlorophyll raw material 10 is poured into the first tank 23 in which the chlorophyll raw material 10 is stored.
  • the chlorophyll raw material 10 and the alcohol 11 are sufficiently stirred by the stirring blades 21 in the first tank 23 to produce a mixed liquid 42 in which the chlorophyll raw material 10 and the alcohol 11 are mixed.
  • the mixed solution 42 is stirred in the first tank 23 for a predetermined time, it is transferred from the first tank 23 to the second tank 24.
  • the mixture 42 passes through the common pipe 35,
  • the ultrasonic vibration means 34 facilitates the extraction of chlorophyll in the chlorophyll raw material 10.
  • the mixed solution 42 is repeatedly transferred between the first tank 23 and the second tank 24, and the ultrasonic vibration means 34 causes the cell wall of the mulberry leaf plant cells contained in the chlorophyll raw material 10. And the cell membrane is crushed and chlorophyll can be eluted in alcohol 11.
  • the extraction of chlorophyll can be promoted, and the work time required for extracting chlorophyll can be shortened (above, the extraction process).
  • the mixed liquid thus obtained is recovered, for example, by opening the third valve 38 and the discharge valve 43 at the bottom of the second tank 24. Since this mixed liquid contains the fiber in the alcohol 11, the chlorophyll-containing substance 12, and the chlorophyll raw material 10, the fiber is first separated.
  • a filtration method may be used for the separation of the fiber content, in this embodiment, a centrifugal separation method using a centrifugal separator 44 having a hemp bag therein is used.
  • the liquid component that is, the filtrate 45 which is a chlorophyll-containing solution, and the residue 46 containing the fiber component which is a solid component are separated.
  • the liquid separated by using the centrifuge 44 is stored in a sedimentation tank, and solid matter in the liquid that cannot be separated by the centrifuge 44 is precipitated to increase the purity of the liquid. Fiber separation treatment).
  • the filtrate 45 is put into an evaporator (evaporator) 47 having a closed structure, and the inside is evacuated. Then, the filtrate 45 in the evaporator 47 is stirred for about 5 to 10 hours (preferably, 6 to 9 hours) by the stirrer 48 while heating the filtrate 45 in the temperature range of 40 to 50 ° C. at a low temperature by hot water bathing. Thereby, the alcohol 11 contained in the filtrate 45 can be separated, that is, removed by evaporation. The removed alcohol 11 is repeatedly used for extracting the chlorophyll.
  • the mixed solution from which the alcohol 11 has been removed contains the chlorophyll-containing substance 12, the water-soluble off-flavor containing ammonia attached to the chlorophyll-containing substance 12, and the water contained in the chlorophyll raw material 10. Have been.
  • the used chlorophyll raw material 10 contains an appropriate amount of water, and the amount of water contained in the mixed solution from which the alcohol 11 has been removed is a specified amount, for example, 0.1 times or more the volume of the mixed solution. 0.3 times or less (e.g., 10 liters of kneaded material with moisture of 1 liter or more and 3 liters or less) If so, the following process is performed using this as a kneaded material 14. However, when the amount of water contained in the used chlorophyll raw material 10 is too small and the amount of water contained in the mixed solution from which the alcohol 11 has been removed cannot satisfy the above-mentioned prescribed amount, the insufficient water 13 is used so as to reach the prescribed amount.
  • the chlorophyll-containing material 12 is stirred without mixing, and the added water is dispersed in the chlorophyll-containing material 12 to obtain a kneaded material 14.
  • the mixing of the water 13 and the chlorophyll-containing substance 12 is sufficient to wash the chlorophyll-containing substance 12 with the added water 13, and vigorously agitate the fine particles to disperse the chlorophyll-containing substance 13 in water. To do.
  • the water 13 to be added to the chlorophyll-containing substance 12 can be water that does not contaminate bacteria and the like in the chlorophyll-containing substance 12, for example, distilled water, ion-exchanged water, or pure water. Solvent removal treatment).
  • the kneaded material 14 obtained in the first step is transferred from a lower part of an evaporator 47 to a container 49, and then placed in a cooler (not shown). Then, the kneaded material 14 is placed in a temperature range higher than 0 ° C and lower than the temperature at which the oil and fat becomes solid, that is, more than 0 ° C (preferably, 2 ° C or more) and less than 10 ° C (preferably, While cooling to 15 ° C or less, the sample is allowed to stand in a cooler for about 3 to 20 hours (more preferably, 5 to 10 hours). At this time, since the water does not solidify, the off-flavor contained in the chlorophyll-containing material 12 is dissolved in the water 16, and the fat and oil contained in the chlorophyll-containing material 12 becomes solid.
  • the chlorophyll-containing material 17 may have an unpleasant odor
  • the chlorophyll-containing material 17 may be washed and the purpose of use of the chlorophyll-containing material 17 may be increased in order to increase the removal rate of the offensive odor. Repeat one or more times, depending on.
  • the chlorophyll-containing substance 17 in which the oils and fats are solidified is put into a container 49, and is liquefied while being heated to a temperature range of 35-50 ° C by hot water decoction. And then liquefy by heating Water having the same composition as the water 13 is added to the chlorophyll-containing material 17 and the mixture is weakly stirred so that the chlorophyll-containing material 17 is not emulsified.
  • the amount of water added should be 0.1-0.3 times the volume of the mixture of chlorophyll-containing substance 17 and water.
  • chlorophyll is not used to confine an off-flavor using an enzyme or the like, as in the conventional chlorophyll-containing material. Since the off-flavor is removed from the element containing chlorophyll, the generation of off-odor from the substance containing chlorophyll can be suppressed and further prevented.
  • the chlorophyll-containing substance 17 produced by the above-mentioned method can be used, for example, in any one or more of beverages, food additives, cosmetic materials, powders and tablets.
  • the chlorophyll-containing substance 17 in which the oils and fats have become a solid substance obtained in the third step is liquefied by heating it in a temperature range of, for example, 35-50 ° C., for example, with a water-insoluble power S.
  • the humidified soil extract water which has bactericidal and antibacterial properties to the liquefied chlorophyll-containing material (hereinafter, also simply referred to as humus extract water) 50, is converted to a stock solution and is 1/5000 or more of the weight of the chlorophyll-containing material 17. After adding 1/20 or less, stir and mix using a stirrer or the like such that the humus extract water 50 is evenly dispersed in the chlorophyll-containing material 17.
  • the humus extract water 50 used is a stock solution containing 4.6 mg / liter of humic acids calculated based on nitrohumic acid.
  • the total organic carbon (TOC) content of the humus extract water 50 is 32 mg / liter.
  • humus soil extraction water for example, the one disclosed in Japanese Patent Application Laid-Open No. 2000-136140 can be used. Hereinafter, the manufacturing method will be briefly described.
  • the humus used in the production of this humus extract water is not limited to the place where it is collected, but it is particularly preferable to use humus collected from Karabi Nishi, Moriyama-machi, Kitakoko-gun, Nagasaki Prefecture. .
  • the collected humus soil contains moisture and is sticky like soil taken from a paddy field. Extraction is performed after two-stage drying of coarse drying and precision drying. In this drying, the humus is dried and fragmented, and at the same time, the function of aerobic microorganisms is activated and the humus is disturbed. It is thought that the action of aerial bacteria is suppressed, and solar sterilization is performed, and at the same time, for example, amino acids, vitamins, and enzymes are activated (ripened) by absorption of ultraviolet rays (photosynthesis). Thereby, it is considered that a beneficial effect of, for example, an antioxidant effect or an anticorrosive effect is exerted.
  • the constituent components are homogenized, mud other than humus is removed, and stable corroded soil extraction water with less variation in pH during extraction can be obtained.
  • the rough drying described above is performed by placing a collected humus soil with a diameter of about 5 to 50 cm on concrete or the like that does not mix with the humus, in a rainy or sunny condition (sunshine), and It is done by drying for one year from now. As a result, the humus becomes a dry mass. Then, it is further dried in a greenhouse (precision drying). This drying is carried out for a minimum of, for example, about one month, usually for about 1.5 to two months. As a result, the drying of the lump further proceeds, and the lump is further pulverized by a cultivator or the like to make the lump of sand.
  • the humus soil extraction water 50 used in the present embodiment is obtained by extracting the above-mentioned humus soil with water.
  • the water used for extraction is not particularly limited, and for example, groundwater, well water, tap water, purified water, or distilled water can be used.
  • the extraction is performed by stirring the humus and water.
  • the temperature at the time of extraction is usually room temperature, and the stirring time is usually 113 hours, preferably 2-3 hours.
  • the mixing ratio of humus and water is 1: 1 to 1: 5, even 1: 3 to 1: 5, especially 1: 5 (weight / volume ratio).
  • the suspension is allowed to stand for 2 to 4 weeks, preferably about 3 weeks to settle suspended particles, and then the supernatant is separated, aspirated, or filtered through a 50-100 / im bag filter. Separate by the separation method used.
  • a suction port is placed 5 to 10 cm above the interface between the supernatant and the humus layer to aspirate the extract, so that the supernatant can be obtained in high yield without sucking the humus layer. Can be sucked.
  • the extract is filtered using a filter of 0.3 am or less, preferably 0.2 ⁇ m or less, more preferably 0.1 ⁇ m or less.
  • a filter of 0.3 am or less preferably 0.2 ⁇ m or less, more preferably 0.1 ⁇ m or less.
  • humus soil extraction water 50 having bactericidal and antibacterial properties can be produced.
  • the chlorophyll-containing material 17 to which the humus soil extraction water 50 has been added is dispersed and mixed in purified water 52 in an emulsified state using an emulsifying device 51.
  • the configuration of the emulsification device 51 is substantially the same as that of the chlorophyll extraction device 20 described above, and therefore, the same members will be denoted by the same reference numerals and will be described below.
  • the chlorophyll-containing substance 17 to which humus soil extraction water 50 has been added and the clean water 52 are charged into the first tank 23 (or the second tank 24) of the emulsifier 51 so as to have a predetermined ratio.
  • the chlorophyll-containing substance 17 and the clean water are stirred and mixed by rotating the stirring blade 21 through the mouth 29 and rotating the stirring blade 21.
  • the predetermined ratio is such that the chlorophyll-containing material 17 and the clean water are, for example, in a volume ratio of about 1:10 to 1: 200.
  • water produced using a reverse osmosis membrane can be used, and pure water from which bacteria, organic substances, metal ions, and nonmetal ions have been removed, or close to pure water It is preferably purified water.
  • This purified water is obtained by using a reverse osmosis membrane having a large number of very fine (for example, 0.0001 ⁇ ⁇ ) pores, for example, from which bacteria such as Escherichia coli, nitrogen compounds, and phosphorylated compounds have been removed. is there.
  • the clean water examples include natural water that has been sterilized to remove impurities (natural water containing a mineral component that is compatible with chlorophyll), water from which residual chlorine has been removed, heated water from which impurities have been removed, or tap water. Water (water that has been sterilized to remove impurities and chlorine) can also be used.
  • the first and second valves 31 and 36 are opened, and the mixed liquid is pumped through the first communication pipe provided with the pump 33 and the ultrasonic vibrating means 34.
  • the first communication pipe is formed by the first communication branch pipe 32, the common pipe 35, and the second communication branch pipe 37 described above.
  • ultrasonic dispersion treatment is performed by the ultrasonic vibration means 34 provided in the common pipe 35, and the chlorophyll-containing material 17 is crushed into small particles to be emulsified and dispersed in clean water, thereby promoting the emulsified state. .
  • the ultrasonic vibrating means 34 can generate an emulsion by applying ultrasonic waves of, for example, 20,000 Hz or more to the mixed liquid to emulsify the fat and oil and the clean water.
  • the third and fourth valves 38 and 40 are opened, and then the first and second valves are immediately opened. 31, 36 are closed.
  • the mixed liquid is further stirred and mixed by the stirring blades 22 in the second tank 24, and the pump 33 and the ultrasonic vibrating means 34 are transferred from the second tank 24 to the first tank 23. It is returned, that is, transported back through the second communication pipe provided.
  • the second communication pipe is formed by the third communication branch pipe 39, the shared pipe 35, and the fourth communication branch pipe 41 described above.
  • ultrasonic dispersion processing is performed by ultrasonic vibration means 34 provided on the way, and emulsification of the mixed solution is promoted.
  • the chlorophyll beverage can also be produced by the following method.
  • the humus soil extraction water and the clean water to be used are the same as those used in the above-mentioned method for producing a chlorophyll beverage, and therefore the description thereof will be omitted.
  • an emulsifier of 10 to 15% by mass of the weight of the chlorophyll-containing material 17 is added.
  • the emulsifier for example, a conventionally known emulsifier can be used, and particularly, an emulsifier produced from a natural material of soybean oil or castor oil is preferably used.
  • the chlorophyll-containing material 17 to which the emulsifier is added is stirred and mixed to liquefy the oils and fats in the chlorophyll-containing material 17. Then, after adding the humus extract water 50 to the liquefied chlorophyll-containing material 17 at 1/5000 or more and 1/20 or less of the weight of the chlorophyll-containing material 17, the humus extract water 50 is added to the chlorophyll-containing material 17. Stir and mix with a stirrer so as to be evenly dispersed.
  • this concentrate is added to the clean water so that the mixing ratio of the chlorophyll-containing substance 17 and the clean water in the concentrate is about 1:10 to 1: 200 by volume, and then the mixture is stirred and mixed.
  • This makes it possible to produce a chlorophyll drink having a quality free from the possibility of an unpleasant odor, having a bactericidal property and an antibacterial property and capable of being stored for a long period of time while maintaining freshness.
  • the chlorophyll-containing substance 17 in which the fat or oil obtained in the above-mentioned third step has become a solid substance is liquefied by heating it in a temperature range of, for example, 3550 ° C. without baking water. Then, after adding the humus extract water 50 described above to the liquefied chlorophyll-containing material 17, the weight of the chlorophyll-containing material is 1Z5000 or more and 1/20 or less, the humus extract water 50 is added to the chlorophyll-containing material 17. Stir and mix using a stirrer or the like so as to be evenly dispersed.
  • the chlorophyll-containing substance 17 to which the humus extract water 50 has been added is kneaded with other components constituting the cosmetic and used.
  • the fiber content of the chlorophyll raw material 10 is used.
  • humus soil extraction water 50 is added to the chlorophyll raw material 10 together with the alcohol 11, and the chlorophyll extraction device 20 performs an extraction treatment.
  • the amount of humus soil extraction water 50 to be added is, for example, 1 / 5000-1 / 20 of the weight of the chlorophyll-containing substance 17 obtained in the final (third step).
  • the chlorophyll-containing substance 12 and the residue 46 containing the fiber content are both sterilized and antibacterial treated.
  • the humus extract water does not need to be diluted with water.
  • the mixed liquid from which chlorophyll has been extracted by the chlorophyll extractor 20 is centrifuged 44 to collect and collect the residue 46 (which is composed of a large amount of fiber and contains a small amount of alcohol 11). Shape.
  • the chlorophyll-containing substance 17 obtained in the third step is added to the mixture, and the mixture is subjected to, for example, kneading 55 and molding 56 to obtain a tablet 57.
  • the chlorophyll-containing substance 17 is added to the powdery residue 46, and the mixture is processed into, for example, kneading 55, drying 58, and pulverizing 59 to form a powder, which is stored in a force capsule to store the strength of the food in a power storage type. It can be 60 or food-caro.
  • the mixed solution from which the chlorophyll extraction has been completed by the chlorophyll extractor 20 is centrifuged by a centrifuge 44. It is also possible to use the evaporator 47 to remove the alcohol 11 without removing the alcohol 11 without removing the fibers. At this time, humus soil extraction water 50 is added to the mixed solution charged into the evaporator 47 so as to be 1/5000 or more and 1/20 or less of the weight of the chlorophyll-containing material 17 obtained in the third step. As a result, the chlorophyll-containing substance 12 containing the fiber component subjected to the sterilization and the antibacterial treatment is recovered.
  • the chlorophyll-containing substance 17 produced through the second and third steps is kneaded and molded together with the fiber component into a powder or a tablet.
  • the chlorophyll-containing material 17 produced through the first to third steps and the residue 46 separated by the centrifugal separator 44 in the first step are mechanically stirred using a stirring means such as a mixer. It is also possible to mix them. At this time, the humus soil extraction water 50 is added so as to be 1/5000 or more and 1/20 or less of the weight of the chlorophyll-containing material 17.
  • the chlorophyll-containing substance 17, the residue 46, and the humus extraction water 50 are mechanically mixed using a mixer, and the mixture is processed into a tablet by powdering and molding.
  • the chlorophyll extraction device 20 was operated, and a chlorophyll extraction process was performed while irradiating a mixture of the chlorophyll raw material 10 and the alcohol 11 with ultrasonic waves at, for example, an output of 1500 watts and a frequency of 20,000 Hz.
  • the mixed solution was recovered from the second tank 24 and centrifuged 44 to separate the residue 46.
  • the filtrate 45 from which the residue 46 has been separated is put into an evaporator 47, and the inside of the evaporator 47 is evacuated to a reduced pressure. For about 69 hours. As a result, a mixed liquid of 910 litters from which the alcohol 11 contained in the filtrate 45 was removed by evaporation was collected. Note that the mixed solution from which alcohol 11 has been removed contains about 2 liters of water. The following treatment was carried out as a kneaded material 14 without adding water to the mixed solution (the first step).
  • the kneaded material 14 obtained in the first step was transferred to a container 49, and then placed in a cooler. Then, the kneaded material 14 was cooled 15 within a temperature range of 2 ° C. or more and less than 10 ° C., and was allowed to stand in a cooler for, for example, about 3 to 10 hours (the above, the second step).
  • the container 49 was taken out of the cooler, and the chlorophyll-containing substance 17 in which the fat content was solidified was taken out of the container 49. As a result, the chlorophyll-containing substance 17 from which the off-flavor was removed was obtained (the above is the third step).
  • the chlorophyll-containing material 17 obtained in this manner was used to produce chlorophyll drinks by using the following methods (Experimental Examples 1 and 2).
  • the chlorophyll-containing substance 17 was heated to, for example, 45 ° C. while being boiled in water, and liquefied. Then, the liquefied chlorophyll-containing material 17 was used as the humus extract water 50 of FX21 (a stock solution containing humic acids calculated on the basis of nitrohumic acid of 4.6 mg / litre) prepared by Nippon Humini Dangaku Co., Ltd. After 1/20 of the weight of the chlorophyll-containing substance 17 was further diluted 5-fold with water, the mixture was stirred and mixed by a stirrer.
  • FX21 a stock solution containing humic acids calculated on the basis of nitrohumic acid of 4.6 mg / litre
  • Example 1 After completion of the stirring and mixing, this was poured into the first tank 23 of the emulsifier 51 in which about 500 liters of clean water 52 was stored, for example, and emulsified to produce a chlorophyll beverage 53 (experimental).
  • Example 1 If the product is an ultra-concentrated ampoule, the amount of clean water 52 is 100 liters, and the amount of clean water 52 is changed according to the product (the same applies to Experimental Example 2 below).
  • the chlorophyll drinks of Experimental Examples 1 and 2 and the chlorophyll drink of the conventional example were stored in a refrigerator set at a room temperature of 10 ° C, and were tested for bacteria, malodor, discoloration, and spoilage for 12 weeks ( (March).
  • the chlorophyll drinks of Experimental Examples 1 and 2 were negative for various bacteria even after 12 weeks, had a mild taste without bitterness and odor, and had no bad odor, discoloration, and rot. It was confirmed that the beverage had stable quality.
  • the chlorophyll drink of the comparative example had bitterness and odor from the beginning, and after 6 weeks, had a bad smell, and had caused discoloration and decay. Unless the temperature of the chlorophyll drink was controlled, an odor could be generated even in one day. At the time of manufacture, although there were no problems with foods at the time of manufacture, the bacteria became positive as the storage period became longer.
  • the silkworm dung can be washed with clean water in advance, sterilized and treated with humus extracted water, dried, ground, and used.
  • Additives eg, sweeteners
  • the oils and fats in the chlorophyll-containing material can be efficiently and quickly converted to solids and separated from water containing off-flavors.
  • humic soil extraction water having bactericidal and antibacterial properties is added to chlorophyll-containing substances, beverages, food additives, cosmetics, powders, and tablets that do not have a problem of offensive odor and can maintain stable quality for a long period of time Can be manufactured.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Insects & Arthropods (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Non-Alcoholic Beverages (AREA)

Abstract

Processus de production d'une substance contenant de la chlorophylle par extraction de la chlorophylle à partir d'un matériau brut (10) contenant de la chlorophylle consistant essentiellement en des excréments de vers à soie et des vers à soie pulvérisés et en retirant les matières nauséabondes de la substance (12) obtenue contenant de la chlorophylle. La substance produite contenant de la chlorophylle peut être stockée tout en maintenant sa fraîcheur. Le processus comprend: une première phase comprenant l'extraction d'une chlorophylle à partir d'une matière brute (10) contenant de la chlorophylle avec un solvant volatile (11) pour préparer un mélange liquide, la séparation des fibres et du solvant (11) du mélange liquide pour obtenir une substance (12) contenant de la chlorophylle et des graisses, et en y ajoutant de l'eau (13) en quantité nécessaire, suivies d'un malaxage pour obtenir un mélange (14); une deuxième phase lors de laquelle le mélange (14) est refroidi (15) et maintenu à une température supérieure à 0°C et inférieure à la température à laquelle les graisses se solidifient pour ainsi déposer l'eau (16) et faire que les matières nauséabondes solubles dans l'eau y compris l'ammoniaque contenues dans la substance (12) contenant de la chlorophylle se dissolvent dans l'eau (16); et une troisième phase lors de laquelle l'eau (16) est retirée pour obtenir une substance (17) contenant de la chlorophylle, de laquelle les matières nauséabondes ont été retirées.
PCT/JP2004/008223 2004-06-11 2004-06-11 Processus de production d'une substance contenant de la chlorophylle Ceased WO2005120255A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP2004/008223 WO2005120255A1 (fr) 2004-06-11 2004-06-11 Processus de production d'une substance contenant de la chlorophylle

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2004/008223 WO2005120255A1 (fr) 2004-06-11 2004-06-11 Processus de production d'une substance contenant de la chlorophylle

Publications (1)

Publication Number Publication Date
WO2005120255A1 true WO2005120255A1 (fr) 2005-12-22

Family

ID=35502751

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2004/008223 Ceased WO2005120255A1 (fr) 2004-06-11 2004-06-11 Processus de production d'une substance contenant de la chlorophylle

Country Status (1)

Country Link
WO (1) WO2005120255A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009256396A (ja) * 2008-04-11 2009-11-05 Toppan Printing Co Ltd 建造物用機能性樹脂
CN104886735A (zh) * 2015-05-14 2015-09-09 孙绍科 一种蚕蛹去皮机

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003038128A (ja) * 2001-07-26 2003-02-12 Hiromi Taguchi 鰻の骨を原料とする健康補助食品及び食品添加物の製造方法
WO2003015539A1 (fr) * 2001-08-13 2003-02-27 Susumu Takayama Procede de production d'une boisson contenant de la chlorophylle
JP2004057105A (ja) * 2002-07-30 2004-02-26 Susumu Takayama 葉緑素飲料及びその製造方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003038128A (ja) * 2001-07-26 2003-02-12 Hiromi Taguchi 鰻の骨を原料とする健康補助食品及び食品添加物の製造方法
WO2003015539A1 (fr) * 2001-08-13 2003-02-27 Susumu Takayama Procede de production d'une boisson contenant de la chlorophylle
JP2004057105A (ja) * 2002-07-30 2004-02-26 Susumu Takayama 葉緑素飲料及びその製造方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009256396A (ja) * 2008-04-11 2009-11-05 Toppan Printing Co Ltd 建造物用機能性樹脂
CN104886735A (zh) * 2015-05-14 2015-09-09 孙绍科 一种蚕蛹去皮机

Similar Documents

Publication Publication Date Title
JP6371229B2 (ja) 水生種の加工のための方法およびシステム
JP6946279B2 (ja) マイクロクロップからタンパク質及び高炭水化物産生物を抽出するための方法及び系並びにその組成物
JP2018532424A (ja) 微小作物から高濃度タンパク質生成物を処理するための方法およびシステム、ならびにその組成物
JP7280313B2 (ja) 水生生物種からシュウ酸が低減されたタンパク質を抽出するための方法及び系並びにその組成物。
CN110799040A (zh) 一种用于制备高多酚橄榄油的方法和设备
KR101633545B1 (ko) 해조류를 이용한 사료의 제조방법
JP2008178864A (ja) 酒類粕の固液分離方法およびそれらの固液分離装置
DE4431394C1 (de) Verfahren zur Kaltgewinnung von Klarsaft, Trub und Öl aus Sanddornbeeren und ihre Verwendung
JP6569994B2 (ja) 蜂産物エキスの製造方法及び蜂産物エキス
KR20210011611A (ko) 인삼 농축액에서의 지용성의 잔류농약의 제거는 물론 프탈레이트계의 가소제를 제거하면서도 쓴맛과 향을 최소화하는 추출물의 제조방법
WO2005120255A1 (fr) Processus de production d'une substance contenant de la chlorophylle
US20080057166A1 (en) Method for extracting highly condensed calcium powder from salted anchovies and the highly condensed calcium powder by obtaining therefrom
CN105558255B (zh) 蓝莓叶肽营养品及其制备方法
KR100629927B1 (ko) 엽록소 음료의 제조 방법
US20050037117A1 (en) Method for producing beverage having chlorophyll
JP3766673B2 (ja) 葉緑素飲料の製造方法
JP3577489B2 (ja) 葉緑素飲料の製造方法
KR101952275B1 (ko) 프로폴리스 추출물 및 추출방법
JP3755093B2 (ja) 合成肝油の製造方法
RU2764298C1 (ru) Система и способ переработки ядер из семян конопли
ES2327087B2 (es) Metodo para separar la membrana interna de la cascara del huevo.
JP3393132B2 (ja) 細胞膜の破壊されたクロレラの製造法
WO2002062161A1 (fr) Procede et appareil permettant de produire une boisson saine contenant de la chlorophylle
JP2011093865A (ja) 褐藻カジメ（学名：ＥｃＫＬｏｎｉａｃｏｖａＫｊｉｌｌｍｅｎ）の藻体からフコイダンを含む抽出液を採取する方法。
JP2007238907A (ja) 天然緑色色素

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: DE

WWW Wipo information: withdrawn in national office

Country of ref document: DE

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP