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WO2005003389A3 - Amplification in vitro de l'adn - Google Patents

Amplification in vitro de l'adn Download PDF

Info

Publication number
WO2005003389A3
WO2005003389A3 PCT/GB2004/002787 GB2004002787W WO2005003389A3 WO 2005003389 A3 WO2005003389 A3 WO 2005003389A3 GB 2004002787 W GB2004002787 W GB 2004002787W WO 2005003389 A3 WO2005003389 A3 WO 2005003389A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
unclonable
dna fragment
vitro amplification
amplifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2004/002787
Other languages
English (en)
Other versions
WO2005003389A2 (fr
Inventor
George Dickinson
Vanessa Hill
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Royal Holloway and Bedford New College
Original Assignee
Royal Holloway and Bedford New College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Royal Holloway and Bedford New College filed Critical Royal Holloway and Bedford New College
Publication of WO2005003389A2 publication Critical patent/WO2005003389A2/fr
Publication of WO2005003389A3 publication Critical patent/WO2005003389A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention porte sur une méthode d'amplification d'un fragment d'ADN inclonable in vitro, cette méthode consistant à circulariser le fragment d'ADN inclonable, ajouter une polymérase de l'ADN, un mélange de dNTP et une amorce, et amplifier le fragment d'ADN inclonable en effectuant une amplification en cercle afin de produire une série en tandem de répétitions du fragment d'ADN inclonable.
PCT/GB2004/002787 2003-06-28 2004-06-25 Amplification in vitro de l'adn Ceased WO2005003389A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0315160A GB0315160D0 (en) 2003-06-28 2003-06-28 In vitro amplification of DNA
GB0315160.2 2003-06-28

Publications (2)

Publication Number Publication Date
WO2005003389A2 WO2005003389A2 (fr) 2005-01-13
WO2005003389A3 true WO2005003389A3 (fr) 2005-03-10

Family

ID=27676273

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2004/002787 Ceased WO2005003389A2 (fr) 2003-06-28 2004-06-25 Amplification in vitro de l'adn

Country Status (2)

Country Link
GB (1) GB0315160D0 (fr)
WO (1) WO2005003389A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006063355A2 (fr) * 2004-12-11 2006-06-15 Cytogenix , Inc. Biosynthese acellulaire d'acide nucleique de haute qualite et utilisations correspondantes
US9139849B2 (en) 2005-04-08 2015-09-22 The United States of America as Represented by the Government of the Department of Health and Human Services Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation
WO2007018744A2 (fr) * 2005-08-03 2007-02-15 Cytogenix, Inc. Biosynthese sans cellule d'acide nucleique
GB0614825D0 (en) * 2006-07-26 2006-09-06 Medical Res Council Method for amplification of ligation reactions
JP2008289483A (ja) 2007-05-25 2008-12-04 Symphogen As 真核生物系において発現可能な形質転換体のスクリーニング
US10077459B2 (en) 2016-05-04 2018-09-18 General Electric Company Cell-free protein expression using rolling circle amplification product
WO2021055760A1 (fr) * 2019-09-18 2021-03-25 Intergalactic Therapeutics, Inc. Vecteurs d'adn synthétiques et procédés d'utilisation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999049079A1 (fr) * 1998-03-25 1999-09-30 Ulf Landegren Replication en cercle roulant destinee a des sondes cadenas
US20030118998A1 (en) * 2001-10-15 2003-06-26 Dean Frank B. Nucleic acid amplification

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999049079A1 (fr) * 1998-03-25 1999-09-30 Ulf Landegren Replication en cercle roulant destinee a des sondes cadenas
US20030118998A1 (en) * 2001-10-15 2003-06-26 Dean Frank B. Nucleic acid amplification

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DEAN F B ET AL: "Rapid amplification of plasmid and phage DNA using Phi29 DNA polymerase and multiply-primed rolling circle amplification", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 11, no. 6, June 2001 (2001-06-01), pages 1095 - 1099, XP002223174, ISSN: 1088-9051 *
DETTER JOHN C ET AL: "Isothermal strand-displacement amplification applications for high-throughput genomics.", GENOMICS, vol. 80, no. 6, December 2002 (2002-12-01), pages 691 - 698, XP002310697, ISSN: 0888-7543 *
DHULIPALA R ET AL: "New amplification technologies for DNA preparation.", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 103, 2003, & 103RD AMERICAN SOCIETY FOR MICROBIOLOGY GENERAL MEETING; WASHINGTON, DC, USA; MAY 18-22, 2003, pages I - 081 URL, XP009041694, ISSN: 1060-2011 *
GUO W X ET AL: "Cloning and expression of full length mouse utrophin: the differential association of utrophin and dystrophin with AChR clusters.", FEBS LETTERS. 2 DEC 1996, vol. 398, no. 2-3, 2 December 1996 (1996-12-02), pages 259 - 264, XP002311207, ISSN: 0014-5793 *
NELSON JOHN R ET AL: "TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing.", SUPPLEMENT TO BIOTECHNIQUES, no. June, 2002, pages S44 - S47, XP001204520 *
NELSON JOHN R: "Phi29 DNA polymerase-based methods for genomics applications.", JOURNAL OF CLINICAL LIGAND ASSAY, vol. 25, no. 3, October 2002 (2002-10-01), pages 276 - 279, XP009041683, ISSN: 1081-1672 *

Also Published As

Publication number Publication date
WO2005003389A2 (fr) 2005-01-13
GB0315160D0 (en) 2003-08-06

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