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WO2005001133A3 - Compositions and methods for high throughput construction and functional analysis of expression libraries - Google Patents

Compositions and methods for high throughput construction and functional analysis of expression libraries Download PDF

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Publication number
WO2005001133A3
WO2005001133A3 PCT/US2004/017494 US2004017494W WO2005001133A3 WO 2005001133 A3 WO2005001133 A3 WO 2005001133A3 US 2004017494 W US2004017494 W US 2004017494W WO 2005001133 A3 WO2005001133 A3 WO 2005001133A3
Authority
WO
WIPO (PCT)
Prior art keywords
high throughput
compositions
expression libraries
functional analysis
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2004/017494
Other languages
French (fr)
Other versions
WO2005001133A2 (en
Inventor
Hong Zhu
Denise M Hickey
Leigh J Hsu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Collateral Therapeutics Inc
Original Assignee
Collateral Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Collateral Therapeutics Inc filed Critical Collateral Therapeutics Inc
Publication of WO2005001133A2 publication Critical patent/WO2005001133A2/en
Publication of WO2005001133A3 publication Critical patent/WO2005001133A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides methods and compositions for rapid construction and high throughput functional analysis of expression libraries. More particularly, the invention provides methods for the rapid construction of expression libraries comprising transcription cassettes and use of such transcription cassettes in high throughput assay systems for the identification and validation of transcription and expression products relevant to drug development.
PCT/US2004/017494 2003-06-03 2004-06-03 Compositions and methods for high throughput construction and functional analysis of expression libraries Ceased WO2005001133A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47567603P 2003-06-03 2003-06-03
US60/475,676 2003-06-03

Publications (2)

Publication Number Publication Date
WO2005001133A2 WO2005001133A2 (en) 2005-01-06
WO2005001133A3 true WO2005001133A3 (en) 2005-04-28

Family

ID=33551558

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/017494 Ceased WO2005001133A2 (en) 2003-06-03 2004-06-03 Compositions and methods for high throughput construction and functional analysis of expression libraries

Country Status (1)

Country Link
WO (1) WO2005001133A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10829772B2 (en) 2012-06-22 2020-11-10 Monsanto Technology Llc Unique modular vector design
CN108949919B (en) * 2018-07-17 2021-07-20 华南理工大学 A dual-mode nucleic acid detection method for aggregation-induced luminescence/surface plasmon colorimetric analysis
CN112680485A (en) * 2021-01-20 2021-04-20 江南大学 Method for converting L-valine into alpha-ketovaline by immobilized recombinant escherichia coli

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999043843A1 (en) * 1998-02-27 1999-09-02 Johns Hopkins University School Of Medicine A simplified system for generating recombinant adenoviruses
US6340595B1 (en) * 1998-06-12 2002-01-22 Galapagos Genomics N.V. High throughput screening of gene function using adenoviral libraries for functional genomics applications

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999043843A1 (en) * 1998-02-27 1999-09-02 Johns Hopkins University School Of Medicine A simplified system for generating recombinant adenoviruses
US6340595B1 (en) * 1998-06-12 2002-01-22 Galapagos Genomics N.V. High throughput screening of gene function using adenoviral libraries for functional genomics applications

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
"APPLICATIONS NUCLEOSPIN MULTI-96 EXTRACT", XP002305058, Retrieved from the Internet <URL:http://www-mn-net.com/catalog/WebE/CWIK-4N2ACE> [retrieved on 20041111] *
"GWIZ-LUC EXPRESSION SYSTEM: PRODUCT SUMMARY AND VECTOR MAP", XP002305059, Retrieved from the Internet <URL:http://www.genetherapysystems.com/upload/gWiz_Luc_Vector_P030200.PDF> [retrieved on 20041111] *

Also Published As

Publication number Publication date
WO2005001133A2 (en) 2005-01-06

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