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WO2005087915A3 - Use of ethanolamine for enhancing cell growth in membrane systems - Google Patents

Use of ethanolamine for enhancing cell growth in membrane systems Download PDF

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Publication number
WO2005087915A3
WO2005087915A3 PCT/US2005/007926 US2005007926W WO2005087915A3 WO 2005087915 A3 WO2005087915 A3 WO 2005087915A3 US 2005007926 W US2005007926 W US 2005007926W WO 2005087915 A3 WO2005087915 A3 WO 2005087915A3
Authority
WO
WIPO (PCT)
Prior art keywords
ethanolamine
cell growth
membrane systems
enhancing cell
serum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2005/007926
Other languages
French (fr)
Other versions
WO2005087915A2 (en
Inventor
Michael Gramer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biovest International Inc
Original Assignee
Biovest International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biovest International Inc filed Critical Biovest International Inc
Publication of WO2005087915A2 publication Critical patent/WO2005087915A2/en
Publication of WO2005087915A3 publication Critical patent/WO2005087915A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention is based, in part, on the discovery that cell growth, cell viability, and antibody production in a membrane system such as hollow fiber system can be enhanced by culturing cells in the presence of serum on the cell side and in a medium containing ethanolamine in the absence of serum on the non-cell side. Accordingly, the present invention provides methods for the enhanced growth of cells while minimizing the use of serum.
PCT/US2005/007926 2004-03-08 2005-03-08 Use of ethanolamine for enhancing cell growth in membrane systems Ceased WO2005087915A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55111904P 2004-03-08 2004-03-08
US60/551,119 2004-03-08

Publications (2)

Publication Number Publication Date
WO2005087915A2 WO2005087915A2 (en) 2005-09-22
WO2005087915A3 true WO2005087915A3 (en) 2006-06-01

Family

ID=34976297

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/007926 Ceased WO2005087915A2 (en) 2004-03-08 2005-03-08 Use of ethanolamine for enhancing cell growth in membrane systems

Country Status (1)

Country Link
WO (1) WO2005087915A2 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602007012238D1 (en) 2006-05-22 2011-03-10 Biovest Internat Inc YEARS
EP2500413A1 (en) 2006-09-13 2012-09-19 Abbott Laboratories Cell culture improvements
WO2008109674A2 (en) 2007-03-05 2008-09-12 Caridianbct, Inc. Cell expansion system and methods of use
EP2148922B1 (en) 2007-04-13 2020-03-04 Terumo BCT, Inc. Cell expansion system and methods of use
CN105154394B (en) 2010-05-05 2019-06-18 泰尔茂比司特公司 The method of the attached cell grown in renewed vaccination hollow-fiber bioreactor system
WO2012048298A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Methods and systems of growing and harvesting cells in a hollow fiber bioreactor system with control conditions
WO2012171030A2 (en) 2011-06-10 2012-12-13 Biovest International, Inc. Method and apparatus for antibody production and purification
WO2012171026A2 (en) 2011-06-10 2012-12-13 Biovest International, Inc. Methods for high yield virus production
US9175259B2 (en) 2012-08-20 2015-11-03 Terumo Bct, Inc. Method of loading and distributing cells in a bioreactor of a cell expansion system
CA2920957A1 (en) 2012-08-28 2014-03-06 Biovest International, Inc. Biomanufacturing suite and methods for large-scale production of cells, viruses, and biomolecules
US9725768B2 (en) 2012-08-31 2017-08-08 Biovest International, Inc. Methods for producing high-fidelity autologous idiotype vaccines
SG11201505965TA (en) * 2013-01-31 2015-09-29 Ajinomoto Kk Culture method for stable undifferentiated proliferation of pluripotent stem cells
WO2015073918A1 (en) 2013-11-16 2015-05-21 Terumo Bct, Inc. Expanding cells in a bioreactor
US11008547B2 (en) 2014-03-25 2021-05-18 Terumo Bct, Inc. Passive replacement of media
US10077421B2 (en) 2014-04-24 2018-09-18 Terumo Bct, Inc. Measuring flow rate
WO2016049421A1 (en) 2014-09-26 2016-03-31 Terumo Bct, Inc. Scheduled feed
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
US11629332B2 (en) 2017-03-31 2023-04-18 Terumo Bct, Inc. Cell expansion
CN109897880A (en) * 2019-03-18 2019-06-18 郑州伊美诺生物技术有限公司 In vitro culture prepares the method and its application of AFP monoclonal antibody

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
GRAMER MICHAEL J ET AL: "Optimal NSO cell growth in a hollow fiber bioreactor requires increased serum concentration or a cholesterol supplement on the cell side of the fiber.", BIOTECHNOLOGY PROGRESS, vol. 19, no. 6, 2003, pages 1762 - 1766, XP002366086, ISSN: 8756-7938 *
INOUE YUICHI ET AL: "Production of recombinant human monoclonal antibody using ras-amplified BHK-21 cells in a protein-free medium", BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 60, no. 5, 1996, pages 811 - 817, XP002366085, ISSN: 0916-8451 *
KLERX J P A M ET AL: "IN-VITRO PRODUCTION OF MONOCLONAL ANTIBODIES UNDER SERUM-FREE CONDITIONS USING A COMPACT AND INEXPENSIVE HOLLOW FIBER CELL CULTURE UNIT", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 111, no. 2, 1988, pages 179 - 188, XP002366082, ISSN: 0022-1759 *
MICHAEL J. GRAMER ET AL.: "Screening tool for hollow-fiber bioreactor process development", BIOTECHNOL. PROG., vol. 14, 1998, pages 203 - 209, XP002366087 *
TAKAZAWA Y ET AL: "HIGH-DENSITY CULTURE OF MOUSE-HUMAN HYBRIDOMA IN SERUM-FREE DEFINED MEDIUM", BIOTECHNOLOGY AND BIOENGINEERING, vol. 31, no. 2, 1988, pages 168 - 172, XP002366084, ISSN: 0006-3592 *
VAN ERP R ET AL: "MONITORING OF THE PRODUCTION OF MONOCLONAL ANTIBODIES BY HYBRIDOMAS PART I. LONG-TERM CULTIVATION IN HOLLOW FIBER BIOREACTORS USING SERUM-FREE MEDIUM", JOURNAL OF BIOTECHNOLOGY, vol. 20, no. 3, 1991, pages 235 - 248, XP002366083, ISSN: 0168-1656 *

Also Published As

Publication number Publication date
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