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WO2005079531A3 - Method and system for eluting cells - Google Patents

Method and system for eluting cells Download PDF

Info

Publication number
WO2005079531A3
WO2005079531A3 PCT/US2005/005490 US2005005490W WO2005079531A3 WO 2005079531 A3 WO2005079531 A3 WO 2005079531A3 US 2005005490 W US2005005490 W US 2005005490W WO 2005079531 A3 WO2005079531 A3 WO 2005079531A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
eluting cells
eluting
materials
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2005/005490
Other languages
French (fr)
Other versions
WO2005079531A2 (en
Inventor
James P Landers
Jerome P Ferrance
Jessica C Voorhees
Kiev R Blasier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UVA Licensing and Ventures Group
University of Virginia UVA
Original Assignee
University of Virginia UVA
University of Virginia Patent Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Virginia UVA, University of Virginia Patent Foundation filed Critical University of Virginia UVA
Publication of WO2005079531A2 publication Critical patent/WO2005079531A2/en
Anticipated expiration legal-status Critical
Publication of WO2005079531A3 publication Critical patent/WO2005079531A3/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to method and system for eluting cells from materials for analysis and diagnostic examination by means of a swab, clothing items, or the like. The method includes incubating the cell-containing material in a solution that degrades the material while maintaining the cells or biological materials of interest, especially polynucleotides, such as DNA and/or RNA, intact. Preferably, the solution contains at least one enzyme for degrading the material. A system for practicing the method is also disclosed.
PCT/US2005/005490 2004-02-19 2005-02-22 Method and system for eluting cells Ceased WO2005079531A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US54608004P 2004-02-19 2004-02-19
US60/546,080 2004-02-19

Publications (2)

Publication Number Publication Date
WO2005079531A2 WO2005079531A2 (en) 2005-09-01
WO2005079531A3 true WO2005079531A3 (en) 2006-12-14

Family

ID=34886237

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/005490 Ceased WO2005079531A2 (en) 2004-02-19 2005-02-22 Method and system for eluting cells

Country Status (1)

Country Link
WO (1) WO2005079531A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8685747B2 (en) 2008-08-15 2014-04-01 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Acting For And On Behalf Of Arizona State University Porous materials for biological sample collection
US10519434B2 (en) 2012-07-13 2019-12-31 Diomics Corporation Biologic sample collection devices and methods of production and use thereof
US10346671B2 (en) 2014-08-15 2019-07-09 Diomics Corporation Films for biologic analyte collection and analysis and methods of production and use thereof
JP2017536087A (en) 2014-09-24 2017-12-07 グッド スタート ジェネティクス， インコーポレイテッド Process control to increase the robustness of genetic assays
US11207677B2 (en) 2018-03-07 2021-12-28 University Of Virginia Patent Foundation Devices, systems, and methods for detecting substances

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5603351A (en) * 1995-06-07 1997-02-18 David Sarnoff Research Center, Inc. Method and system for inhibiting cross-contamination in fluids of combinatorial chemistry device
US5623415A (en) * 1995-02-16 1997-04-22 Smithkline Beecham Corporation Automated sampling and testing of biological materials
US6184011B1 (en) * 1999-03-22 2001-02-06 Cbd Technologies, Ltd Method of releasing solid matrix affinity adsorbed particulates

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5623415A (en) * 1995-02-16 1997-04-22 Smithkline Beecham Corporation Automated sampling and testing of biological materials
US5603351A (en) * 1995-06-07 1997-02-18 David Sarnoff Research Center, Inc. Method and system for inhibiting cross-contamination in fluids of combinatorial chemistry device
US6184011B1 (en) * 1999-03-22 2001-02-06 Cbd Technologies, Ltd Method of releasing solid matrix affinity adsorbed particulates

Also Published As

Publication number Publication date
WO2005079531A2 (en) 2005-09-01

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