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WO2005044972A2 - Support tridimensionnel de culture de substance microbiologique - Google Patents

Support tridimensionnel de culture de substance microbiologique Download PDF

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Publication number
WO2005044972A2
WO2005044972A2 PCT/DK2004/000771 DK2004000771W WO2005044972A2 WO 2005044972 A2 WO2005044972 A2 WO 2005044972A2 DK 2004000771 W DK2004000771 W DK 2004000771W WO 2005044972 A2 WO2005044972 A2 WO 2005044972A2
Authority
WO
WIPO (PCT)
Prior art keywords
carrier
carriers
carrier according
plane
culturing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DK2004/000771
Other languages
English (en)
Other versions
WO2005044972A3 (fr
Inventor
Peter Esser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nunc AS
Original Assignee
Nunc AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nunc AS filed Critical Nunc AS
Publication of WO2005044972A2 publication Critical patent/WO2005044972A2/fr
Publication of WO2005044972A3 publication Critical patent/WO2005044972A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06—Plates; Walls; Drawers; Multilayer plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10—Rotating vessel
    • C12M27/12—Roller bottles; Roller tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/30—Details relating to random packing elements
    • B01J2219/302—Basic shape of the elements
    • B01J2219/30207—Sphere
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/30—Details relating to random packing elements
    • B01J2219/302—Basic shape of the elements
    • B01J2219/30238—Tetrahedron
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/30—Details relating to random packing elements
    • B01J2219/302—Basic shape of the elements
    • B01J2219/30242—Star
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/30—Details relating to random packing elements
    • B01J2219/302—Basic shape of the elements
    • B01J2219/30246—Square or square-derived
    • B01J2219/30253—Pyramid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/30—Details relating to random packing elements
    • B01J2219/302—Basic shape of the elements
    • B01J2219/30296—Other shapes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/30—Details relating to random packing elements
    • B01J2219/304—Composition or microstructure of the elements
    • B01J2219/30466—Plastics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/30—Details relating to random packing elements
    • B01J2219/318—Manufacturing aspects
    • B01J2219/3183—Molding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags

Definitions

  • the present invention relates to a three-dimensional carrier for culturing microbiological material, comprising at least two substantially plane, intersecting surfaces with a substantially circular circumference.
  • Cell cultures are used for the production of a vast variety of products, and various methods and apparatus exist for the cultivation of cultures of bacterial and fungal cells, and cells from higher organisms.
  • cells are cultured in containers such as so-called roller bottles, i.e. cylindrical vessels, usually of glass, polystyrene, or polyethyleneterephthalate, which are partially filled with a liquid medium comprising the cells.
  • roller bottles i.e. cylindrical vessels, usually of glass, polystyrene, or polyethyleneterephthalate
  • the bottles are rotated slowly about their longitudinal axes, promoting aeration, while the liquid medium provides nutrients to the cells as they grow on the inner surface of the container.
  • anchorage dependent cells For cells requiring a support for their growth (anchorage dependent cells), i.e. they must attach to a surface or a solid substratum in order to be able to grow, the relatively small surface area provided by the interior of the container limits the efficiency of conventional growth vessels.
  • a roller bottle is disclosed with a rough or uneven polymeric inner cell growth surface for culturing cells.
  • the growth surface is formed as an integral part of the roller bottle.
  • chitosan beads are disclosed having relatively large and uniform pores increasing their surface area for cell growth.
  • a carrier of a biocompatible material for the culturing of anchorage dependent cells is provided.
  • the carrier is a three-dimensional carrier for culturing microbiological material, comprising at least two substantially plane, intersecting surfaces with a substantially circular circumference.
  • a three-dimensional object means an object that extends in three mutually perpendicular directions in space with dimensions in the same order of magnitude, i.e. for a three-dimensional object it is not possible to choose three mutually perpendicular directions in space where the extension in one direction is insignificant or infinitesimal in relation to the other directions, i.e. no directions can be found where the extension of the object is less than 1/10'th of the extension in the other directions.
  • the substantially circular circumference of the intersecting surfaces imparts a substantially spherical geometry to the carrier, i.e. the carrier may be perceived to be a solid sphere or a solid polyhedron with cut-outs providing the plane surfaces.
  • the substantially spherical geometry of the carrier allows dense packaging of carriers inside a container, such as a roller bottle, a flask, a dish, a well plate, a tray, a cell bag, an elution column, etc, whereby the growth area in the container is increased considerably.
  • the geometry of the carriers according to the present invention minimizes interference between surfaces of neighbouring carriers when packed.
  • the roller bottle is filled with carriers so that individual carriers cannot move when the bottle is rotated. Abutment of two carriers typically takes place along the thin outer circumference of the carriers leaving the plane growth surface of each carrier substantially unblocked by other carriers.
  • the substantially spherical geometry allows the carrier to roll.
  • the carrier comprises at least three substantially plane, intersecting surfaces with a substantially circular circumference.
  • three substantially plane and intersecting surfaces constitute the carrier.
  • the three axes of intersection are substantially mutually perpendicular and even more preferred, the plane surfaces are also substantially mutually perpendicular. Capillary attraction in the volume defined between neighbouring plane surfaces of a carrier makes nutrition liquid available across the entire growth surface including the inner corners of the volume.
  • a further embodiment of the present invention comprises five substantially plane, intersecting surfaces with a substantially circular circumference whereby the growth area may be further increased.
  • the carrier is made of a material that withstands sterilization, such as, e.g., sterilization by irradiation (beta or gamma radiation), steam autoclave, ethylene oxide, chemical disinfectants, or dry heat sterilization.
  • sterilization such as, e.g., sterilization by irradiation (beta or gamma radiation), steam autoclave, ethylene oxide, chemical disinfectants, or dry heat sterilization.
  • the carrier is made from a polymeric material that is processable by injection moulding and biocompatible, preferably hydrophilic.
  • materials that are suitable for use in the present context are e.g., polyethylene, polypropylene, polystyrene, polycarbonate, polyurethane, polysulfone, polymethylpentene, polymethylmetacrylate, polyethyleneterephthalate, polytetrafluoroethylene, cycloolefin copolymers, or ABS (acrylonitrilbutadiene styrene).
  • the carriers are made of polystyrene by injection moulding.
  • substrates need to be surface treated after moulding in order to make the surface hydrophilic and to enhance the likelihood for effective cell attachment.
  • Surface treatment may take the form of a surface coating, but typically involves the use of directed energy at the substrate surface with the intention of generating chemical groups on the polymer surface. These chemical groups will have a general affinity for water or otherwise exhibit sufficient polarity to permit stable adsorption to another polar group. These functional groups lead to hydrophilicity and/or an increase in surface oxygen and are properties recognised to enhance cell growth.
  • the carrier may have a diameter up to 10 mm.
  • the carrier walls are very thin, i.e.
  • the wall thickness is around 1/10'th of the carrier diameter, more preferred less than 1/10'th the carrier diameter provided that the carrier material has the required strength.
  • the carriers are small, since the smaller the carrier the easier it is to maintain the carriers in a suspension in aqueous media, i. e. with slow stirring or even without stirring.
  • the ratio between the surface area provided by the carrier and the volume occupied by the carrier increases with decreasing carrier diameter provided that the thickness of the carrier walls is kept significantly less than the carrier diameter.
  • the carrier diameter is 7 mm, or less than 7 mm, such as 4 mm or 2 mm.
  • Micro sized carriers may be provided according to the present invention with a diameter that is less than 1 mm, such as 0,75 mm, 0,50 mm, 0,25 mm, etc.
  • the surfaces of the carriers according to the invention are continuous surfaces with no holes or apertures making the entire area available for cell growth.
  • the carrier material of a density close to 1.0 g/ml such as in the range from 0.90 g/ml to 1.1 g/ml, from 0.93 g/ml to 1.07 g/ml, from 0.95 g/ml to 1.05 g/ml, from 0.98 g/ml to 1.02 g/ml, is preferably selected, e.g. cycloolefin copolymers have a density of app.
  • polymethylpentene has a density that is less than 1.0 g/ml.
  • a carrier density close to or equal to the density of the nutrition liquid minimizes the need for agitation. Agitation requirements may be further decreased by utilization of small carriers.
  • Various polymers may be mixed in order to obtain a desired density, e.g. as mentioned above.
  • the carrier material has a density that is less than and close to the density of the nutrition liquid. Thereby the carrier material tends to move towards the surface of the liquid medium, which may be desirable for growth of certain microbiological material.
  • the carrier material has a density that is larger than and close to the density of the nutrition liquid. Thereby the carrier material tends to move away from the surface of the liquid medium, which may be desirable for growth of certain microbiological material.
  • cells are detached from the carrier by well-known methods, e.g. by means of trypsine or ethylenediamine tetraacetate (EDTA).
  • EDTA ethylenediamine tetraacetate
  • V Sbfar 2 - 4br + b 2 )
  • the growth surface of a standard 12 cm 0 roller bottle may be increased by a factor of more than 22 by packing the bottle with carriers having these characteristics.
  • the person skilled in the art will realise that similar advantages may be obtained in other containers for growth of biological substances, e.g. in a flask, a dish, a well plate, a tray, a tray stack, a cell bag, etc.
  • substantially circular circumference is intended to cover all shapes resembling a circle or a part of a circle, such as an elliptical shape, a parabolic shape, a hyperbolic shape, a polygonal shape, etc.
  • it is important that the circumference of the substantially plane surfaces does not match or fit into the volume defined between neighbouring plane surfaces of a carrier, since a fit would make it possible for abutting carriers to engage with each other in such a way that significant parts of a surface of one carrier may be blocked by an abutting surface of another carrier.
  • the circumference is polygonal, the angles of the polygon should be different from the angle between intersecting surfaces. Fig.
  • FIG. 1 shows a first substantially spherical embodiment of the invention
  • Fig. 2 shows a second substantially spherical embodiment of the invention
  • Fig. 3 shows a third substantially spherical embodiment of the invention
  • Fig. 4 shows a fourth substantially spherical embodiment of the invention
  • Fig. 5 shows a fifth substantially polyhedral embodiment of the invention.
  • Fig. 1 shows a first embodiment 10 of a carrier according to the present invention.
  • the carrier is made of polystyrene by injection moulding followed by plasma surface treatment.
  • the carrier 10 is a three-dimensional carrier comprising three substantially plane, intersecting surfaces 12, 14, 16, each of which has a substantially circular circumference 18, 20, 22 extending 360° around the respective surface 12, 14, 16.
  • the substantially circular circumference 18, 20, 22 of the intersecting surfaces 12, 14, 16 imparts a substantially spherical geometry to the carrier 10, i.e. the carrier may be perceived to be a solid sphere with 8 cut-outs, 4 of which are visible with reference numerals 24, 26, 32, 34, providing the plane surfaces.
  • the three axes 28, 29, 30 of intersection are substantially perpendicular to each other. Capillary attraction in the 8 volumes, 4 of which are visible with reference numerals 24, 26, 32, 34, defined between neighbouring plane surfaces 12, 14, 16 of the carrier 10 makes nutrition liquid available across the entire growth surface including the inner corners 36 of the volume 24, 26, 32, 34.
  • Fig. 2 shows a second embodiment 10 of a carrier according to the present invention, corresponding to the carrier of Fig. 1 , wherein the part above the horizontal plane 14 has been turned 45° in relation to the part below the horizontal plane 14, as indicated by the arrow 11.
  • the circumference 22 still extends 360° around the surface 14 while the other circumferences 20 extend 180° around the respective surfaces 16.
  • Fig. 3 shows a third embodiment 10 of a carrier according to the present invention, comprising five substantially plane, intersecting surfaces 12, 14, 16, 38, 40 with a substantially circular circumference whereby the growth area may be further increased.
  • Fig. 4 shows a fourth embodiment 10 of a carrier according to the present invention, comprising four substantially plane, intersecting surfaces 14, 42, 44, 46 with a substantially circular circumference. It should be noted that contrary to the embodiments shown in Figs. 1 and 2, surfaces 42, 44, 46 do not extend on both sides of their mutual intersection 48.
  • Fig. 5 shows a fifth embodiment 10 of a carrier according to the present invention, resembling the embodiment shown in Fig. 3 apart from the fact that the plane surfaces have polygonal circumferences. It should be noted that the carrier of Fig. 5 might be perceived to be a solid polyhedron with cut-outs providing the plane surfaces.
  • the carriers shown in the figures have a diameter of 7 mm and an average wall thickness of 0,6 mm.
  • the carrier of Fig. 1 has a surface area of 1 ,8 cm 2 and may be packed to a density of more than 4000 carriers/litre. This means that the capacity of a common sized roller bottle having a 12 cm diameter may be increased from about 0,15 m 2 to more than 3,3 m 2 , i.e. by a factor of more than 22.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un support d'une substance biocompatible destiné à la culture de cellules dépendantes d'un support. Le support est un support tridimensionnel permettant de cultiver une substance microbiologique et comprenant au moins deux surfaces sensiblement planes se croisant et présentant une circonférence sensiblement circulaire. Celle-ci confère une géométrie sensiblement sphérique au support, telle que le support peut sembler être une sphère solide ou un polyèdre solide doté de découpes mettant en place les surfaces planes. La géométrie sensiblement sphérique du support permet d'emballer de nombreux supports dans un contenant, tel qu'une bouteille pour agitateur rotatif, un flacon, un godet, une plaque à cupules, un plateau, un sachet de cellules, une colonne d'élution, etc., la zone de croissance dans le contenant étant accrue sensiblement. De plus, la géométrie des supports permet de minimiser des interférences entre des surfaces de supports voisins quand ils sont emballés et la géométrie sensiblement sphérique permet aux supports de rouler.
PCT/DK2004/000771 2003-11-06 2004-11-05 Support tridimensionnel de culture de substance microbiologique Ceased WO2005044972A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200301650 2003-11-06
DKPA200301650 2003-11-06
US53617804P 2004-01-12 2004-01-12
US60/536,178 2004-01-12

Publications (2)

Publication Number Publication Date
WO2005044972A2 true WO2005044972A2 (fr) 2005-05-19
WO2005044972A3 WO2005044972A3 (fr) 2005-06-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2004/000771 Ceased WO2005044972A2 (fr) 2003-11-06 2004-11-05 Support tridimensionnel de culture de substance microbiologique

Country Status (1)

Country Link
WO (1) WO2005044972A2 (fr)

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WO2010088916A2 (fr) 2009-02-05 2010-08-12 Origo Biotech Aps Polyèdres courbés
US8778669B2 (en) 2009-07-22 2014-07-15 Corning Incorporated Multilayer tissue culture vessel
US9005550B2 (en) 2012-10-29 2015-04-14 Corning Incorporated Multi-layered cell culture vessel with manifold grips
EP2902478A1 (fr) * 2012-09-06 2015-08-05 Pluristem Ltd. Dispositifs et procédés pour la culture de cellules
WO2015132729A1 (fr) * 2014-03-04 2015-09-11 Pluristem Ltd. Systèmes et procédés de culture et de collecte de cellules
US11608486B2 (en) 2015-07-02 2023-03-21 Terumo Bct, Inc. Cell growth with mechanical stimuli
US11613727B2 (en) 2010-10-08 2023-03-28 Terumo Bct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
US11629332B2 (en) 2017-03-31 2023-04-18 Terumo Bct, Inc. Cell expansion
US11634677B2 (en) 2016-06-07 2023-04-25 Terumo Bct, Inc. Coating a bioreactor in a cell expansion system
US11667876B2 (en) 2013-11-16 2023-06-06 Terumo Bct, Inc. Expanding cells in a bioreactor
US11667881B2 (en) 2014-09-26 2023-06-06 Terumo Bct, Inc. Scheduled feed
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11795432B2 (en) 2014-03-25 2023-10-24 Terumo Bct, Inc. Passive replacement of media
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US12043823B2 (en) 2021-03-23 2024-07-23 Terumo Bct, Inc. Cell capture and expansion
US12152699B2 (en) 2022-02-28 2024-11-26 Terumo Bct, Inc. Multiple-tube pinch valve assembly
FR3152125A1 (fr) * 2023-08-18 2025-02-21 Pierre Calleja Dispositif de traitement d’un flux gazeux comprenant au moins un support d’un milieu liquide dans lequel sont présents des micro-organismes ou des microparticules
US12234441B2 (en) 2017-03-31 2025-02-25 Terumo Bct, Inc. Cell expansion
WO2025076586A1 (fr) * 2023-10-09 2025-04-17 Hydgene Renewables Pty Ltd Système générateur
USD1099116S1 (en) 2022-09-01 2025-10-21 Terumo Bct, Inc. Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device

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Cited By (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010088916A3 (fr) * 2009-02-05 2010-09-23 Origo Biotech Aps Polyèdres courbés
US20120040455A1 (en) * 2009-02-05 2012-02-16 Origo Biotech Aps Curved polyhedrons
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