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WO2004111267A3 - Methodes de preparation d'une bibliotheque de reserves pourvues de plaques ayant une resolution inferieure a une megabase et utilisations - Google Patents

Methodes de preparation d'une bibliotheque de reserves pourvues de plaques ayant une resolution inferieure a une megabase et utilisations Download PDF

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Publication number
WO2004111267A3
WO2004111267A3 PCT/CA2004/000859 CA2004000859W WO2004111267A3 WO 2004111267 A3 WO2004111267 A3 WO 2004111267A3 CA 2004000859 W CA2004000859 W CA 2004000859W WO 2004111267 A3 WO2004111267 A3 WO 2004111267A3
Authority
WO
WIPO (PCT)
Prior art keywords
pools
library
nucleic acid
submegabase
preparation
Prior art date
Application number
PCT/CA2004/000859
Other languages
English (en)
Other versions
WO2004111267A2 (fr
Inventor
Wan Lam
Calum Macaulay
Spencer Watson
Adrian Ishkanian
Martin Krzywinski
Marco Marra
Original Assignee
Bc Cancer Agency
Wan Lam
Calum Macaulay
Spencer Watson
Adrian Ishkanian
Martin Krzywinski
Marco Marra
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bc Cancer Agency, Wan Lam, Calum Macaulay, Spencer Watson, Adrian Ishkanian, Martin Krzywinski, Marco Marra filed Critical Bc Cancer Agency
Priority to CA002570068A priority Critical patent/CA2570068A1/fr
Publication of WO2004111267A2 publication Critical patent/WO2004111267A2/fr
Publication of WO2004111267A3 publication Critical patent/WO2004111267A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries

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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un procédé de préparation de réserves de fragments d'acides nucléiques synthétiques pouvant être à nouveau remplies si nécessaire. Chacune des réserves représente un clone d'un ensemble de plaques de clones génomiques englobant sensiblement par chevauchement un génome entier, ou une partie de celui-ci. Les réserves de fragments obtenues par cette méthode peuvent être à nouveau remplies à tout moment sans nécessité d'isoler à nouveau les clones génomiques originaux utilisés pour créer l'ensemble de plaques. Les réserves peuvent être utilisées, par exemple, comme source de sondes dans des applications telles que l'hybridation d'acides nucléiques ou FISH. Les bibliothèques peuvent être utilisées, par exemple, dans la préparation de jeux d'échantillons d'acides nucléiques pouvant représenter un génome entier ou une partie de celui-ci.
PCT/CA2004/000859 2003-06-12 2004-06-14 Methodes de preparation d'une bibliotheque de reserves pourvues de plaques ayant une resolution inferieure a une megabase et utilisations WO2004111267A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002570068A CA2570068A1 (fr) 2003-06-12 2004-06-14 Methodes de preparation d'une bibliotheque de reserves pourvues de plaques ayant une resolution inferieure a une megabase et utilisations

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32026603P 2003-06-12 2003-06-12
US60/320,266 2003-06-12

Publications (2)

Publication Number Publication Date
WO2004111267A2 WO2004111267A2 (fr) 2004-12-23
WO2004111267A3 true WO2004111267A3 (fr) 2005-06-09

Family

ID=33551152

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2004/000859 WO2004111267A2 (fr) 2003-06-12 2004-06-14 Methodes de preparation d'une bibliotheque de reserves pourvues de plaques ayant une resolution inferieure a une megabase et utilisations

Country Status (2)

Country Link
CA (1) CA2570068A1 (fr)
WO (1) WO2004111267A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1576192B1 (fr) 2002-12-23 2014-10-29 Agilent Technologies, Inc. Essais comparatifs d'hybridation de genomes utilisant des oligonucleotides immobilises, et compositions permettant de les mettre en oeuvre
US8321138B2 (en) 2005-07-29 2012-11-27 Agilent Technologies, Inc. Method of characterizing quality of hybridized CGH arrays
US20110301862A1 (en) * 2010-06-04 2011-12-08 Anton Petrov System for array-based DNA copy number and loss of heterozygosity analyses and reporting
CN114842911B (zh) * 2022-06-21 2022-09-20 深圳市睿法生物科技有限公司 基于精准医疗的基因检测流程的优化方法及装置

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000053811A1 (fr) * 1999-03-11 2000-09-14 Orion Genomics, Llc Puces genomiques et cartographie optique de transcrits
WO2001090416A2 (fr) * 2000-05-19 2001-11-29 The Regents Of The University Of California Procedes et compositions de preparation d'un jeu de polynucleotides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000053811A1 (fr) * 1999-03-11 2000-09-14 Orion Genomics, Llc Puces genomiques et cartographie optique de transcrits
WO2001090416A2 (fr) * 2000-05-19 2001-11-29 The Regents Of The University Of California Procedes et compositions de preparation d'un jeu de polynucleotides

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
"AGBT meeting 2003", SOUTHWEST BIOTECHNOLOGY AND INFORMATICS CENTER, XP002310984, Retrieved from the Internet <URL:http://darwin.nmsu.edu/cgi-bin/moreinfo.cgi?10521> *
"Full-genome BAC rearrays. Human genome-high resolution BAC rearray set.", pages 1 - 5, XP002310985, Retrieved from the Internet <URL:http://www.bcgsc.ca/lab/mapping/bacrearray/human> *
ISHKANIAN ADRIAN S ET AL: "A tiling resolution DNA microarray with complete coverage of the human genome.", NATURE GENETICS, vol. 36, no. 3, March 2004 (2004-03-01), pages 299 - 303, XP002310989, ISSN: 1061-4036 *
KRZYWINSKI M. ET AL.: "A set of reaarayed BAC clones spanning the humawn genome", ADVANCES IN GENOME BIOLOGY AND TECHNOLOGY CONFERENCE, 5 February 2003 (2003-02-05), XP002310983, Retrieved from the Internet <URL:http://www.bcgsc.ca/lab/mapping/bacrearray/human> [retrieved on 20041210] *
KRZYWINSKI MARTIN ET AL: "A set of BAC clones spanning the human genome", NUCLEIC ACIDS RESEARCH, vol. 32, no. 12, 9 July 2004 (2004-07-09), pages 3651 - 3660, XP002310990, ISSN: 0305-1048 *
OSOEGAWA KAZUTOYO ET AL: "A bacterial artificial chromosome library for sequencing the complete human genome", GENOME RESEARCH, vol. 11, no. 3, March 2001 (2001-03-01), pages 483 - 496, XP002310986, ISSN: 1088-9051 *
SNIJDERS A M ET AL: "ASSEMBLY OF MICROARRAYS FOR GENOME-WIDE MEASUREMENT OF DNA COPY NUMBER", NATURE GENETICS, NATURE AMERICA, NEW YORK, US, vol. 29, no. 3, November 2001 (2001-11-01), pages 263 - 264, XP001180277, ISSN: 1061-4036 *
SNIJDERS ET AL.: "Web note A (Assembly of microarrays for genome-wide measurement of DNA copy number by CGH).", NATURE GENETICS, 3 November 2001 (2001-11-03), XP002310987 *
WATSON SPENCER K ET AL: "Methods for high throughput validation of amplified fragment pools of BAC DNA for constructing high resolution CGH arrays.", BMC GENOMICS [ELECTRONIC RESOURCE]. 14 JAN 2004, vol. 5, no. 1, 14 January 2004 (2004-01-14), pages 1 - 8, XP002310988, ISSN: 1471-2164 *

Also Published As

Publication number Publication date
CA2570068A1 (fr) 2004-12-23
WO2004111267A2 (fr) 2004-12-23

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