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WO2004111184A3 - Method of amplifying metabolically inactive bacteria - Google Patents

Method of amplifying metabolically inactive bacteria Download PDF

Info

Publication number
WO2004111184A3
WO2004111184A3 PCT/US2003/031382 US0331382W WO2004111184A3 WO 2004111184 A3 WO2004111184 A3 WO 2004111184A3 US 0331382 W US0331382 W US 0331382W WO 2004111184 A3 WO2004111184 A3 WO 2004111184A3
Authority
WO
WIPO (PCT)
Prior art keywords
amplifying
medium
microbes
metabolically inactive
growth
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/031382
Other languages
French (fr)
Other versions
WO2004111184A2 (en
Inventor
Veronique A Dartois
James A Hoch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMBIOSIS PHARMACEUTICALS
Original Assignee
EMBIOSIS PHARMACEUTICALS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EMBIOSIS PHARMACEUTICALS filed Critical EMBIOSIS PHARMACEUTICALS
Priority to AU2003304210A priority Critical patent/AU2003304210A1/en
Publication of WO2004111184A2 publication Critical patent/WO2004111184A2/en
Anticipated expiration legal-status Critical
Publication of WO2004111184A3 publication Critical patent/WO2004111184A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides methods of inducing the growth and multiplication of microbial species. The methods involve incubating a sample of microbes in a growth medium that contains an enrichment medium. The enrichment medium is created by pre-culturing a sample of the microbes in an aliquot of the growth medium. In one embodiment the microbes are dormant or nonculturable bacteria. In preferred embodiments, the enrichment medium is added to the growth medium in an amount of about 5% v/v.
PCT/US2003/031382 2002-10-04 2003-10-03 Method of amplifying metabolically inactive bacteria Ceased WO2004111184A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003304210A AU2003304210A1 (en) 2002-10-04 2003-10-03 Method of amplifying metabolically inactive bacteria

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42111902P 2002-10-04 2002-10-04
US60/421,119 2002-10-04

Publications (2)

Publication Number Publication Date
WO2004111184A2 WO2004111184A2 (en) 2004-12-23
WO2004111184A3 true WO2004111184A3 (en) 2005-06-30

Family

ID=33551197

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/031382 Ceased WO2004111184A2 (en) 2002-10-04 2003-10-03 Method of amplifying metabolically inactive bacteria

Country Status (2)

Country Link
AU (1) AU2003304210A1 (en)
WO (1) WO2004111184A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104302759A (en) * 2012-03-08 2015-01-21 菲仕兰产品有限公司 Culture medium for eukaryotic cells

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4794080A (en) * 1984-04-16 1988-12-27 Igene Biotechnology, Inc. Microbial co-culture production of propionic acid
US5352586A (en) * 1987-05-01 1994-10-04 Biogaia Ab Method of determining the presence of an antibiotic produced by Lactobacillus reuteri
US6251383B1 (en) * 1999-04-20 2001-06-26 National Institute Of Immunology Method for ex-vivo expansion of hematopoietic cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4794080A (en) * 1984-04-16 1988-12-27 Igene Biotechnology, Inc. Microbial co-culture production of propionic acid
US5352586A (en) * 1987-05-01 1994-10-04 Biogaia Ab Method of determining the presence of an antibiotic produced by Lactobacillus reuteri
US6251383B1 (en) * 1999-04-20 2001-06-26 National Institute Of Immunology Method for ex-vivo expansion of hematopoietic cells

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BLOOMFIELD ET AL: "Comment!?", MICROBIOLOGY, vol. 144, January 1998 (1998-01-01), pages 1 - 2 *
BRUNS ET AL: "Cyclic AMP and acyl homoserine lactones increase the cultivation efficiency of heterotrophic bacteria from the central Baltic sea", APPL ENVIRON MICROBIOL, vol. 68, 2002, pages 3978 - 3987 *
GROBBEN ET AL: "Enhancement fo Exopolysaccharide Production of Lactobacillus delbrueckii subsp. bulgaricus NCFB 2722 with a simplified defined medium", APPL ENVIRON MICROBIOL, vol. 64, no. 4, April 1998 (1998-04-01), pages 1333 - 1337 *
KABERLEIN ET AL: "Isolating "uncultivable" microorganisms in pure culture in a simulated natural environment", SCIENCE, vol. 296, 10 May 2002 (2002-05-10), pages 1127 - 1129, XP001206821, DOI: doi:10.1126/science.1070633 *
KELL ET AL: "Bacterial Dormancy and culturability", CURR OPIN MICROBIOL, vol. 3, 2000, pages 238 - 243 *
MUKAMOLOVA ET AL: "A bacterial cytokine", PROC NATL ACAD SCI, vol. 95, July 1998 (1998-07-01), pages 8916 - 8921, XP002080580 *
MUKAMOLOVA ET AL: "Stimulation of the multiplication of Micrococcus luteus by an autocrine growth factor", ARCH MICROBIOL, vol. 172, 1999, pages 9 - 14, XP001070639 *

Also Published As

Publication number Publication date
AU2003304210A1 (en) 2005-01-04
AU2003304210A8 (en) 2005-01-04
WO2004111184A2 (en) 2004-12-23

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