WO2004105797A1

WO2004105797A1 - A pharmaceutical composition comprising a p2x7 antagonist and sulfasalazine

Info

Publication number: WO2004105797A1
Application number: PCT/SE2004/000816
Authority: WO
Inventors: Nigel Boughton-Smith
Original assignee: AstraZeneca AB
Current assignee: AstraZeneca AB
Priority date: 2003-05-29
Filing date: 2004-05-27
Publication date: 2004-12-09
Anticipated expiration: 2005-11-29
Also published as: EP1644041A1; US20070010497A1

Abstract

The invention provides a pharmaceutical composition, pharmaceutical product or kit comprising a first active ingredient which is a P2X7 receptor antagonist, and a second active ingredient which is 2-hydroxy-5-[[4-[(2-pyridinylamino)sulfonyl]phenyl]azo]benzoic acid (sulfasalazine) or a pharmaceutically acceptable derivative thereof, for use in the treatment of inflammatory disorders.

Description

NEW COMBINATION

The present invention relates to combinations of pharmaceutically active substances for use in the treatment of inflammatory conditions/disorders, especially rheumatoid arthritis.

Chronic inflammatory disorders such as rheumatoid arthritis are polygenic, highly complex, and involve multiple inflammatory and immune mechanisms. Treatment of these disorders has been largely empirical with a variety of therapeutic agents being used with little understanding of the mechanisms involved. Recent research suggests that two inflammatory mediators, the cytokines IL-1 and TNFalpha (TNFα), may play key roles in the inflammatory process in rheumatoid arthritis.

It would be desirable to develop new pharmaceuticals for use in treating inflammatory conditions/disorders.

In accordance with the present invention, there is therefore provided a pharmaceutical composition comprising, in admixture, a first active ingredient which is a P2Xγ receptor antagonist, and a second active ingredient which is 2-hydroxy-5-[[4-[(2- pyridinylamino)sulfonyl]phenyl]azo]benzoic acid (sulfasalazine) or a pharmaceutically acceptable derivative thereof.

The P2X receptor (previously known as P2Z receptor) is a ligand-gated ion channel that is present on a variety of cell types, largely those known to be involved in the inflammatory/immune process, specifically, macrophages, mast cells and lymphocytes (T and B). Activation of the P2X7 receptor by extracellular nucleotides, in particular adenosine triphosphate, is known to lead, amongst other things, to the release of interleukin-lβ (IL-lβ).

An antagonist of the P2X7 receptor is a compound or other substance that is capable of preventing, whether fully or partially, activation of the P2X7 receptor. Methods for assaying for P2X7 receptor antagonism are known in the art, for example from WO 01/42194 which describes an assay based on the observation that when the P2X7 receptor is activated using a receptor agonist in the presence of ethidium bromide (a fluorescent DNA probe), an increase in the fluorescence of intracellular DNA-bound ethidium bromide is observed. Thus, an increase in fluorescence can be used as a measure of P2X7 receptor activation and therefore to quantify the effect of a compound or substance on the P2X7 receptor.

In WO 01/42194, the assay is carried out by taking a 96-well flat bottomed microtitre plate and filling the wells with 250 μl of test solution comprising 200 μl of a suspension of

6 -4

THP-1 cells (2.5 x 10 cells/ml) containing 10 M ethidium bromide, 25 μl of a high potassium buffer solution containing 10 M benzoylbenzoyl adenosine triphosphate

(bbATP, a known P2X7 receptor agonist), and 25 μl of the high potassium buffer solution containing 3 x 10 M test compound. The plate is covered with a plastics sheet and incubated at 37 °C for one hour. The plate is then read in a Perkin-Elmer fluorescent plate reader, excitation 520 nm, emission 595 nm, slit widths: Ex 15 nm, Em 20 nm. For the purposes of comparison, bbATP (a P2X7 receptor agonist) and pyridoxal 5-phosphate (a P2X7 receptor antagonist) are used separately in the test as controls. From the readings obtained, a PIC50 figure is calculated for the test compound, this figure being the negative logarithm of the concentration of test compound necessary to reduce the bbATP agonist activity by 50%. A PIC50 figure greater than 5.5 is normally indicative of an antagonist.

Examples of P2X7 receptor antagonists which may be used in accordance with present invention include the compounds described in WO 00/61569, WO 01/42194,

WO 01/44170 and WO 03/41707 the entire contents of which are incorporated herein by reference.

More specifically, in a first embodiment of the present invention the P2X7 receptor antagonist is a compound of formula

wherein m represents 1, 2 or 3;

. la each R independently represents a hydrogen or halogen atom; A^a represents C(O)NH or NHC(O);

Ar represents

X represents a bond, an oxygen atom or a group CO, (CH2)ι-₆, CH=, (CB^ -_όO, O(CH₂)ι_-6, O(CH₂)_2-6O, O(CH₂)2-3θ(CH₂)ι_3, CR'(OH), (CH₂)ι-3θ(CH₂)ι.3, (CH₂)ι-3θ(CH₂)2-3θ, NR^5a, (CH₂)_1-6NR^5a, NR^5a(CH₂)ι_-6, (CH₂)ι.3NR^5a(CH2)ι_-3, O(CH₂)_2-6NR^5a, O(CH₂)_2-3NR^5a(CH2)ι-3, (CH₂)ι.3NR^5a(CH₂)2-3θ, NR^5a(CH₂)₂-6θ, NR^5a(CH₂)₂-₃O(CH2)i_-3, CONR^5a, NR^5aCO, S(O)_n, S(O)_nCH₂, CH₂S(O)_n, SO₂NR^5a or NR^5aSO_{2 ;} n is 0, 1 or 2; R' represents a hydrogen atom or a Ci-Cβ alkyl group;

2a 3a one of R and R represents a halogen, cyano, nitro, amino, hydroxyl, or a group selected from (i) Cj-Cβ alkyl optionally substituted by at least one C3-C6 cycloalkyl,

(ii) C3-C8 cycloalkyl, (iii) Cj-Cg alkyloxy optionally substituted by at least one

C3-C6 cycloalkyl, and (iv) C3-C8 cycloalkyloxy, each of these groups being optionally

2a 3a substituted by one or more fluorine atoms, and the other of R and R represents a hydrogen or halogen atom; either R represents a 3- to 9-membered saturated or unsaturated aliphatic heterocyclic ring system containing one or two nitrogen atoms and optionally an oxygen atom, the heterocyclic ring system being optionally substituted by one or more substituents independently selected from fluorine atoms, hydroxyl, carboxyl, cyano, Cj-C6 alkyl, Cι-C₆ hydroxyalkyl, -NR^6aR^7a, -(CH₂)_rNR^6aR^7a and -CONR^6aR^7a,

4a or R represents a 3- to 8-membered saturated carbocyclic ring system substituted by one or more substituents independently selected from -NR K , -(CH2)_rNR K and

-CONR K. , the ring system being optionally further substituted by one or more substituents independently selected from fluorine atoms, hydroxyl and Cι-C6 alkyl; r is 1, 2, 3, 4, 5 or 6;

5a R represents a hydrogen atom or a Cj-Cό alkyl or C3-C8 cycloalkyl group;

R and R each independently represent a hydrogen atom or a Cj-Cg alkyl,

C2-C6 hydroxyalkyl or C3-C8 cycloalkyl group, or R and R together with the nitrogen atom to which they are attached form a 3- to 8-membered saturated heterocyclic ring; with the provisos that,

(a) when A represents C(O)NH and R represents an unsubstituted 3- to 8-membered a saturated aliphatic heterocyclic ring system containing one nitrogen atom, then X is other than a bond, and a a (b) when A represents C(O)NH and X represents a group (CH2)i-₆ or O(CH2)ι-₆, then

4a R does not represent an unsubstituted imidazolyl, unsubstituted morpholinyl, unsubstituted piperidinyl or unsubstituted pyrrolidinyl group, and

(c) when A represents NHC(O) and R represents an unsubstituted 3- to 8-membered saturated aliphatic heterocyclic ring system containing one nitrogen atom, then X is other than a bond, and

(d) when A^a represents NHC(O) and X^a represents O(CH₂)ι_-6, NH(CH₂)ι-₆ or SCH₂,

4a then R does not represent an unsubstituted 1 -piperidinyl or unsubstituted 1 -pyrrolidinyl group, and

(e) when A^a represents NHC(O) and X^a represents O(CH2)2-₃NH(CH2)₂, then R ^& does not represent an imidazolyl group; or a pharmaceutically acceptable salt or solvate thereof.

Compounds of formula (I) are described in WO 00/61569.

In a second embodiment of the present invention the P2X7 receptor antagonist is a compound of formula

wherein D represents CH2 or CH₂CH2; E^b represents C(O)NH or NHC(O);

R

each independently represent a hydrogen or halogen atom, or an amino, nitro, Ci-Cό alkyl or trifluoromethyl group; R represents a group of formula

R^4b R^5b X Y _(m).

X represents an oxygen or sulphur atom or a group NH, SO or SO2; Y represents an oxygen or sulphur atom or a group NR , SO or SO2; Z represents a group -OH, -SH, -CO2H, Ci-Cβ alkoxy, Ci-Cβ alkylthio, -Cό-alkylsulphinyl, Ci-Cβ-alkylsulphonyl, -NR^6bR^7b, -C(O)NR^8bR^9b, imidazolyl, 1-methylimidazolyl, -N(R )C(O)-Cι-C6 alkyl, Cj-Cό alkylcarbonyloxy,

Cι-C₆ alkoxycarbonyloxy, -OC(O)NR^12bR^13b, -OCH₂OC(O)R^14b, -OCH₂OC(O)OR^15b or -OC(O)OCH₂OR^16b;

4b R represents a C2-C6 alkyl group;

R represents a C1-C6 alkyl group; fib „7b „8b „9b _π10b _ 12b _{J Ω} 13b , . , . ,

R , R , R , R , R , R and R each independently represent a hydrogen atom, or a Ci-Cβ alkyl group optionally substituted by at least one hydroxyl group;

R represents a hydrogen atom, or a Ci-Cβ alkyl group optionally substituted by at least one substituent independently selected from hydroxyl and Cj-Cό alkoxy; and

„ 14b _ 15b _{ι r}, 16b . . . , , _^, ,-, „ ,

R , R and R each independently represent a Ci-Cό alkyl group; with the provisos that (i) when E represents NHC(O), X represents O, S or NH and Y represents O, then Z represents -NR R where R represents a hydrogen atom and

R represents either a hydrogen atom or a Cj-Cό alkyl group substituted by at least one hydroxyl group, and (ii) when E represents NHC(O), X represents O, S or NH, Y represents NH and R represents CH2CH2, then Z is not -OH or imidazolyl; or a pharmaceutically acceptable salt or solvate thereof.

Compounds of formula (II) are described in WO 01/42194.

In a third embodiment of the present invention the P2X7 receptor antagonist is a compound of formula

c wherein D represents CH2 or CH2CH2;

E^C represents C(O)NH or NHC(O); lc 2c

R and R each independently represent hydrogen, halogen, amino, nitro, C -C^ alkyl lc 2c or trifluoromethyl, but R and R may not both simultaneously represent hydrogen;

3c R represents a group of formula 4c 5c

.R^' ._χC ,R

(V);

4c R represents a Ci-Cg alkyl group; c 13c

X represents an oxygen or sulphur atom or a group NR , SO or SO2;

5c 5c

R represents hydrogen, or R represents Ci -C^ alkyl or C2-C6 alkenyl, each of which may be optionally substituted by at least one substituent selected from halogen, hydroxyl, (di)-C₁-C₆-alkylamino, -Y^C-R⁶ ,

a 5- or 6-membered heteroaromatic ring comprising from 1 to 4 heteroatoms independently selected from nitrogen, oxygen and sulphur which heteroaromatic ring may itself be optionally substituted by at least one substituent selected from halogen, hydroxyl and Cι-C₆ alkyl;

Y represents an oxygen or sulphur atom or a group NH, SO or SO2;

6c lc c 7c c

R represents a group -R Z where R represents a C2-Cg alkyl group and Z represents

Re c 10c 1 1 c 12-C an -OH, -CO₂H, -NR R , -C(O)NR R or -N(R )C(O)-C!-C₆ alkyl group, and, c 6c in the case where Y represents an oxygen or sulphur atom or a group NH, R additionally represents hydrogen, Ci-Cβ alkyl, Ci-Cg alkylcarbonyl, Ci-Cg

14c I I fic 17r alkoxycarbonyl, -C(O)NR R , -CH₂OC(O)R , -CH₂OC(O)OR or

-C(O)OCH₂OR ;

8c 9c 10c lie 12c R , R , R , R and R each independently represent a hydrogen atom or a Ci -C^ alkyl group;

13c 13c

R represents hydrogen, C3-C8 cycloalkyl, C3-C8 cycloalkylmethyl, or R represents a Cj-Cg alkyl group optionally substituted by at least one substituent selected from hydroxyl and C1-C6 alkoxy; and R , R , R , R and R each independently represent a Cj-Cό alkyl group; with the proviso that when E° is C(O)NH, X° is O, NH or N(Cι-Cg alkyl), then R ^c is other than a hydrogen atom or an unsubstituted Ci-Cg alkyl group; or a pharmaceutically acceptable salt or solvate thereof. 5c Preferred compounds of formula (IV) are those wherein R represents an optionally c 6c 5 c substituted Ci-Cβ alkyl group, a preferred substituent being -Y -R . When R is substituted with a 5- or 6-memberered heteroaromatic ring comprising from 1 to 4 heteroatoms, it is preferred that the number of heteroatoms in the ring is not greater than 2.

Compounds of formula (IV) are described in WO 01/44170.

In a fourth embodiment of the present invention the P2X7 receptor antagonist is a compound of formula

wherein m represents 1, 2 or 3; . Id . each R independently represents a hydrogen or halogen atom; A^d represents C(O)NH or NHC(O);

. d Ar represents a group

.2d .3d one of R and R represents halogen, nitro, amino, hydroxyl, or a group selected from (i) C -Cζ alkyl optionally substituted by at least one halogen atom,

(ii) C3-C8 cycloalkyl, (iii) C1-C6 alkoxy optionally substituted by at least one halogen atom, and (iv) C₃-C₈ cycloalkyloxy, and the other of

represents a hydrogen or halogen atom; _τ.4d

R represents a group

X represents an oxygen or sulphur atom or a group >N-R ; n is 0 or 1 ;

R represents a C1-C5 alkyl group which may be optionally substituted by at least one substituent selected from hydroxyl, halogen and -Cg alkoxy; fc H

R and R each independently represent a hydrogen atom, Cj-Cβ alkyl (optionally substituted by at least one substituent selected from hydroxyl, halogen, Ci-Cζ alkoxy, and (di)-Cι-C4 alkylamino (itself optionally substituted by at least one hydroxyl group)), or C₃-C₈ cycloalkyl (optionally substituted by at least one substituent selected from hydroxyl, halogen and -Cg alkoxy); and

8d R represents a hydrogen atom or a C1-C5 alkyl group which may be optionally substituted by at least one substituent selected from hydroxyl, halogen and Ci-Cg alkoxy; with the provisos that:

(a) when n is 0, then A is NHC(O), and

(b) when n is l, X represents oxygen and A is C(O)NH, then R and R do not both simultaneously represent a hydrogen atom or do not both simultaneously T ^j HA represent an unsubstituted C1-C6 alkyl, or when one of R and R T represents a hydrogen atom, then the other of R and R does not represent an unsubstituted C -C^ alkyl; and j ^j fZ

(c) when n is 1 , X is oxygen, sulphur or >NH and A is NHC(O), then R and

HA

R do not both simultaneously represent a hydrogen atom or do not both simultaneously represent an unsubstituted Ci-Cβ alkyl, or when one of R and R represents a hydrogen atom, then the other of R and R does not represent an unsubstituted Cj-Cg alkyl or -CH2CH2OH; or a pharmaceutically acceptable salt or solvate thereof. Compounds of formula (VI) are described in WO 03/41707.

In another aspect of the present invention the P2X7 receptor antagonist is a compound of formula

(XI) wherein m represents 1, 2 or 3; e

A represents C(O)NH or NHC(O);

,e

Y represents N or CH;

X^e represents a bond, CO, (CH₂)_1-6, O(CH₂)_1-6, (CH₂)_1-6NH(CH₂)_1-6, (CH₂)_1-6O(CH₂)_1-6, NH(CH₂)_1-6; Z^e represents NR^2eR^{3 e}; le R represents halogen, cyano, nitro, amino, hydroxyl, Ci-Cg alkyl or C3-C8 cycloalkyl, which alkyl or cycloalkyl group group can be optionally substituted by one or more fluorine atoms;

2e 3e

R and R each independently represent a hydrogen atom, C^-Cό alkyl or C3-C8 cycloalkyl, which alkyl or cycloalkyl group can be optionally substituted by one or more groups selected from hydroxyl, halogen or -C^ alkoxy,

2e 3e or R and R together with the nitrogen atom to which they are attached form a 3- to 9- membered saturated mono- or bicyclic heterocyclic ring comprising from 1 to 2 nitrogen atoms and optionally an oxygen atom, which heterocyclic ring can be optionally substituted by one or more groups selected from hydroxyl, halogen or Ci-Cg alkoxy; or a pharmaceutically acceptable salt or solvate thereof.

Compounds of formula (XI) may be prepared by chemistry according or analogous to that described in the references cited herein above.

In a further aspect of the present invention the P2X7 receptor antagonist is:-

2-Chloro-5- [ [2-(2-hydroxy-ethylamino)-ethylamino] -methyl] -N- (tricyclo [3.3.1.1³'⁷] dec- 1 -ylmethyl)-benzamide,

2-Chloro-5-[3-[(3-hydroxypropyl)amino]propyl]-N-(tricyclo[3.3.1.1]dec-l-ylmethyl)- benzamide,

(R)-2-Chloro-5-[3-[(2-hydroxy-l-methylethyl)amino]propyl]-N- (tricyclo[3.3.1. l³'⁷]dec-l-ylmethyl)-benzamide,

2-Chloro-5-[[2-[(2-hydroxyethyl)amino]ethoxy]methyl]-N-(tricyclo[3.3.1.1^3,7]dec- 1 - ylmethyl)-benzamide, 2-Chloro-5-[3-[3-(methylamino)propoxy]propyl]-N-(tricyclo[3.3.1.1³'⁷]dec-l- ylmethyl)benzamide,

2-Chloro-5-[3-(3-hydroxy-propylamino)-propoxy]-N-(tricyclo[3.3.1.1^3,7]dec-l- ylmethyl)-benzamide,

2-Chloro-5-[2-(3-hydroxypropylamino)ethylamino]-N-(tricyclo[3.3.1.1³'⁷]dec-l- ylmethyl)-benzamide,

2-Chloro-5-[2-(3-hydroxypropylsulfonyl)ethoxy]-N-(tricyclo[3.3.1.1³'⁷]dec-l- ylmethyl)-benzamide,

2-Chloro-5-[2-[2-[(2-hydroxyethyl)amino]ethoxy]ethoxy]-N-(tricyclo[3.3.1. l^3,7]dec-l- ylmethyl)-benzamide, 2-Chloro-5-[[2-[[2-(l-methyl-lH-imidazol-4-yl)ethyl]amino]ethyl]amino]-N-

(tricyclo[3.3.1. l³'⁷]dec-l-ylmethyl)-benzamide,

2-Chloro-5-piperazin- 1 -ylmethyl-N-(tricyclo[3.3.1.1] dec- 1 -ylmethyl)-benzamide,

3 7 2-Chloro-5-(4-piperidinyloxy)-N-(tricyclo[3.3.1.1 ' ]dec-l-ylmethyl)-benzamide,

2-Chloro-5-(2,5-diazabicyclo[2.2. l]hept-2-ylmethyl)-N-(tricyclo[3.3.1. l]dec-l- ylmethyl)-benzamide, 3 7 2-Chloro-5-(piperidin-4-ylsulfinyl)-N-(tricyclo[3.3.1.1 ' ]dec-l-ylmethyl)-benzamide,

5-Chloro-2-[3-[(3-hydroxyproρyl)amino]propyl]-N-(tricyclo[3.3.1.1^3,7]dec-l- ylmethyl)-4-ρyridinecarboxamide,

2-Chloro-5-[3-[[( lR)-2-hydroxy- 1 -methylethyl]amino]propyl]-N- (tricyclo[3.3.1. l³'⁷]dec-l-ylmethyl)-3-pyridinecarboxamide,

5-Chloro-2-[3-(ethylamino)propyl]-N-(tricyclo[3.3.1. l^3,7]dec-l-ylmethyl)-4- pyridinecarboxamide,

5-Chloro-2-[3-[(2-hydroxyethyl)amino]ρropyl]-N-(tricyclo[3.3.1.1³'⁷]dec-l-ylmethyl)- 4-pyridinecarboxamide, 5-Chloro-2-[3-[[(25)-2-hydroxypropyl]amino]propyl]-N-(tricyclo[3.3.1.1³'⁷]dec-l- ylmethyl)-4-pyridinecarboxamide,

N-[2-Methyl-5-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-ylcarbonyl)phenyl]- tricyclo[3.3.1. l³'⁷]decane-l-acetamide, or a pharmaceutically acceptable salt or solvate of any one thereof.

Pharmaceutically acceptable salts include, where applicable, acid addition salts derived from pharmaceutically acceptable inorganic and organic acids such as a chloride, bromide, sulphate, phosphate, maleate, fumarate, tartrate, citrate, benzoate, 4-methoxybenzoate, 2- or 4-hydroxybenzoate, 4-chlorobenzoate, p-toluenesulphonate, methanesulphonate, ascorbate, acetate, succinate, lactate, glutarate, gluconate, tricarballylate, hydroxynaphthalene-carboxylate or oleate salt; and salts prepared from pharmaceutically acceptable inorganic and organic bases. Salts derived from inorganic bases include aluminium, ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic, manganous, potassium, sodium, zinc and bismuth salts. Particularly preferred are the ammonium, calcium, magnesium, potassium and sodium salts. Salts derived from pharmaceutically acceptable organic bases include salts of primary, secondary and tertiary amines, cyclic amines like arginine, betaine, choline and the like. Examples of pharmaceutically acceptable solvates include hydrates. Examples of P2X7 receptor antagonists that may conveniently be used in the present invention include:-

2-Chloro-5- [ [2-(2-hydroxy-ethylamino)-ethylamino] -methyl] -N- (tricyclo[3.3.1. l³'⁷]dec-l-ylmethyl)-benzamide, dihydrochloride 2-Chloro-5-[3-[(3-hydroxypropyl)amino]propyl]-N-(tricyclo[3.3.1. l]dec-l-ylmethyl)- benzamide, hydrochloride

(R)-2-Chloro-5-[3-[(2-hydroxy- 1 -methylethyl)amino]propyl]-N- (tricyclo [3.3.1.1³'⁷] dec- 1 -ylmethyl)-benzamide, hydrochloride

2-Chloro-5-[[2-[(2-hydroxyethyl)amino]ethoxy]methyl]-N-(tricyclo[3.3.1.1³'⁷]dec-l- ylmethyl)-benzamide, acetate (1: 1) salt

2-Chloro-5-[3-[3-(methylamino)propoxy]propyl]-N-(tricyclo[3.3.1.1³'⁷]dec-l- ylmethyl)benzamide, hydrochloride

2-Chloro-5-[3-(3-hydroxy-propylamino)-propoxy]-N-(tricyclo[3.3.1. l³'⁷]dec- 1- ylmethyl)-benzamide, hydrochloride 2-Chloro-5-[2-(3-hydroxypropylamino)ethylamino]-N-(tricyclo[3.3.1.1³'⁷]dec-l- ylmethyl)-benzamide, acetate (1: 1) salt

2-Chloro-5-[2-(3-hydroxypropylsulfonyl)ethoxy]-N-(tricyclo[3.3.1.1³'⁷]dec-l- ylmethyl)-benzamide

2-Chloro-5-[2-[2-[(2-hydroxyethyl)amino]ethoxy]ethoxy]-N-(tricyclo[3.3.1.1³'⁷]dec-l- ylmethyl)-benzamide, hydrochloride

2-Chloro-5-[[2-[[2-( 1 -methyl- lH-imidazol-4-yl)ethyl]amino]ethyl]amino]-N- (tricyclo[3.3.1.1³'⁷]dec-l-ylmethyl)-benzamide

2-Chloro-5-piperazin- 1 -ylmethyl-N-(tricyclo[3.3.1.1] dec- 1 -ylmethyl)-benzamide, dihydrochloride

3,7 2-Chloro-5-(4-piperidinyloxy)-N-(tricyclo[3.3.1.1 ' ]dec-l-ylmethyl)-benzamide, hydrochloride

2-Chloro-5-(2,5-diazabicyclo[2.2. l]hept-2-ylmethyl)-N-(tricyclo[3.3.1. l]dec-l- ylmethyl)-benzamide, hydrochloride

2-Chloro-5-(piperidin-4-ylsulfinyl)-N-(tricyclo[3.3.1.1³'⁷]dec-l-ylmethyl)-benzamide 5-Chloro-2-[3-[(3-hydroxyρropyl)amino]proρyl]-N-(tricyclo[3.3.1.1^3,7]dec-l- ylmethyl)-4-pyridinecarboxamide,

2-Chloro-5-[3-[[(lR)-2-hydroxy-l-methylethyl]amino]proρyl]-N- (tricyclo[3.3.1. l³'⁷]dec-l-ylmethyl)-3-pyridinecarboxamide,

5-Chloro-2-[3-(ethylamino)propyl]-N-(tricyclo[3.3.1.1^3,7]dec-l-ylmethyl)-4- pyridinecarboxamide, hydrochloride

5-Chloro-2-[3-[(2-hydroxyethyl)amino]propyl]-N-(tricyclo[3.3.1.1^3,7]dec-l-ylmethyl)- 4-pyridinecarboxamide, hydrochloride

5-Chloro-2-[3-[[(2,S)-2-hydroxypropyl]amino]propyl]-N-(tricyclo[3.3.1.1^3,7]dec-l- ylmethyl)-4-pyridinecarboxamide, dihydrochloride, and

N-[2-Methyl-5-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-ylcarbonyl)phenyl]- tricyclo[3.3.1.1^3,7]decane- 1 -acetamide, hydrochloride.

Sulfasalazine (2-hydroxy-5-[[4-[(2-pyridinylamino)sulfonyl]phenyl]azo]benzoic acid) has the following chemical structure:

In the context of the present specification, unless otherwise stated, a pharmaceutically acceptable derivative of sulfasalazine means a pharmaceutically acceptable ester, salt or solvate of sulfasalazine or a pharmaceutically acceptable solvate of such an ester or salt.

Examples of suitable esters include lower alkyl (Cj-Cg alkyl) esters.

Pharmaceutically acceptable salts include acid addition salts derived from pharmaceutically acceptable inorganic and organic acids such as a chloride, bromide, sulphate, phosphate, maleate, fumarate, tartrate, citrate, benzoate, 4-methoxybenzoate, 2- or 4-hydroxybenzoate, 4-chlorobenzoate, p-toluenesulphonate, methanesulphonate, ascorbate, acetate, succinate, lactate, glutarate, gluconate, tricarballylate, hydroxynaphthalene-carboxylate or oleate salt; and salts prepared from pharmaceutically acceptable inorganic and organic bases. Salts derived from inorganic bases include aluminium, ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic, manganous, potassium, sodium, zinc and bismuth salts. Particularly preferred are the ammonium, calcium, magnesium, potassium and sodium salts. Salts derived from pharmaceutically acceptable organic bases include salts of primary, secondary and tertiary amines, cyclic amines like arginine, betaine, choline and the like.

Examples of pharmaceutically acceptable solvates include hydrates.

The preparation of sulfasalazine is described, for example, in U.S. Patent No. 2,396,145 and by Doras wamy, Guha, I. Indian. Chem. Soc, 23, 278 (1946). Pharmaceutically acceptable derivatives of sulfasalazine may be prepared by methods conventional in the art.

Presently available oral formulations of sulfasalazine include azulfidine and azulfidine EN- Tabs (trade mark) (Pharmacia & Upjohn).

The active ingredients used in the present invention may be capable of existing in stereoisomeric forms. It will be understood that the invention encompasses all geometric and optical isomers of the active ingredients and mixtures thereof including racemates. Tautomers and mixtures thereof also form an aspect of the present invention.

The invention also provides a pharmaceutical product comprising, in combination, a preparation of a first active ingredient which is a P2X7 receptor antagonist, and a preparation of a second active ingredient which is 2-hydroxy-5-[[4-[(2- pyridinylamino)sulfonyl]phenyl]azo]benzoic acid (sulfasalazine) or a pharmaceutically acceptable derivative thereof, for simultaneous, sequential or separate use in therapy.

In another aspect, the invention provides a kit comprising a preparation of a first active ingredient which is a P2X7 receptor antagonist, a preparation of a second active ingredient which is 2-hydroxy-5-[[4-[(2-pyridinylamino)sulfonyl]phenyl]azo]benzoic acid (sulfasalazine) or a pharmaceutically acceptable derivative thereof, and instructions for the simultaneous, sequential or separate administration of the preparations to a patient in need thereof.

It has been found that the choice of active ingredients according to the invention is advantageous because it results in a beneficial anti-inflammatory effect and, accordingly, can be used to treat various acute and chronic inflammatory conditions/disorders such as rheumatoid arthritis.

The pharmaceutical composition of the invention may be prepared by mixing the first active ingredient with the second active ingredient. Therefore, in a further aspect of the present invention, there is provided a process for the preparation of a pharmaceutical composition which comprises mixing a first active ingredient which is a P2X7 receptor antagonist, with a second active ingredient which is 2-hydroxy-5-[[4-[(2- pyridinylamino)sulfonyl]phenyl]azo]benzoic acid (sulfasalazine) or a pharmaceutically acceptable derivative thereof.

The first and second active ingredients may alternatively be administered simultaneously (other than in admixture as described above), sequentially or separately to treat inflammatory conditions. By sequential is meant that the first and second active ingredients are administered, in any order, one immediately after the other. They still have the desired effect if they are administered separately but less than about 4 hours apart, preferably less than about 2 hours apart, more preferably less than about 30 minutes apart. The first and second active ingredients are conveniently administered by oral or parenteral (intaarticular or inhaled) administration using conventional systemic dosage forms, such as tablets, capsules, pills, powders, aqueous or oily solutions or suspensions, emulsions and sterile injectable aqueous or oily solutions or suspensions. These dosage forms will usually include one or more pharmaceutically acceptable ingredients which may be selected, for example, from adjuvants, carriers, binders, lubricants, diluents, stabilising agents, buffering agents, emulsifying agents, viscosity-regulating agents, surfactants, preservatives, flavourings and colorants.

Oral administration is preferred.

For the above-mentioned therapeutic uses the dosages administered will, of course, vary with the first and second active ingredients employed, the mode of administration, the treatment desired and the condition or disorder indicated. However, in general, satisfactory results will be obtained when the total, combined, daily dosage of first and second active ingredients, when taken orally, is in the range from 10 to 2000 milligrammes (mg), particularly from 10, 20, 30, 40, 50, 100, 150, 200 or 300 to 1800, 1500, 1200, 1000, 800, 700, 600, 500 or 400 mg.

The pharmaceutical composition, pharmaceutical product or kit according to the invention may be administered as divided doses from 1 to 4 times a day, and preferably once or twice a day.

The present invention further provides the use of a pharmaceutical composition, pharmaceutical product or kit according to the invention in the manufacture of a medicament for the treatment of an inflammatory disorder.

Also, the present invention provides a method of treating an inflammatory disorder which comprises administering a therapeutically effective amount of a pharmaceutical composition of the invention to a patient in need thereof. Still further, the present invention provides a method of treating an inflammatory disorder which comprises simultaneously, sequentially or separately administering:

(a) a (therapeutically effective) dose of a first active ingredient which is a P2X7 receptor antagonist; and

(b) a (therapeutically effective) dose of a second active ingredient which is 2-hydroxy-5- [[4-[(2-pyridinylamino)sulfonyl]phenyl]azo]benzoic acid (sulfasalazine) or a pharmaceutically acceptable derivative thereof, to a patient in need thereof.

In the context of the present specification, the term "therapy" also includes "prophylaxis" unless there are specific indications to the contrary. The terms "therapeutic" and "therapeutically" should be construed accordingly.

Prophylaxis is expected to be particularly relevant to the treatment of persons who have suffered a previous episode of, or are otherwise considered to be at increased risk of, the condition or disorder in question. Persons at risk of developing a particular condition or disorder generally include those having a family history of the condition or disorder, or those who have been identified by genetic testing or screening to be particularly susceptible to developing the condition or disorder.

The invention further relates to triple combination therapies for the treatment of any one of rheumatoid arthritis, osteoarthritis, osteoporosis, psoriasis, inflammatory bowel diseases, COPD, asthma, allergic rhinitis or cancer or the neurodegenerative diseases such as multiple sclerosis, Alzheimer's disease or stroke.

For the treatment of rheumatoid arthritis, the pharmaceutical composition of the invention may be combined with "biological agents" such as IL-1 receptor antagonists (e.g. Anakinra) and IL-1 trap, IL-18 receptor, anti-IL-6 Ab, anti-CD20 Ab, anti-IL-15 Ab and CTLA4Ig. Suitable agents to be used in combination with the pharmaceutical composition of the invention include standard non-steroidal anti-inflammatory agents (hereinafter NS AID's) such as piroxicam, diclofenac, propionic acids such as naproxen, flubiprofen, fenoprofen, ketoprofen and ibuprofen, fenamates such as mefenamic acid, indomethacin, sulindac, apazone, pyrazolones such as phenylbutazone, salicylates such as aspirin. Cylco-oxygenase inhibiting nitric oxide donors (CINOD' s) and "disease modifying agents" (DMARDs) such as cyclosporine A, leflunomide; ciclesonide; hydroxychloroquine, d-penicillamine, auranofin or parenteral or oral gold may also be used.

The present invention still further relates to the combination of a pharmaceutical composition of the invention together with a leukotriene biosynthesis inhibitor, 5- lipoxygenase (5-LO) inhibitor or 5-lipoxygenase activating protein (FLAP) antagonist selected from the group consisting of zileuton; ABT-761; fenleuton; tepoxalin; Abbott- 79175; Abbott-85761; N-(5-substituted)-thiophene-2-alkylsulfonamides; 2,6-di-tert- butylphenol hydrazones; methoxytetrahydropyrans such as Zeneca ZD-2138; the compound SB-210661; pyridinyl-substituted 2n cyanonaphthalene compounds such as L- 739,010; 2-cyanoquinoline compounds such as L-746,530; indole and quinoline compounds such as MK-591, MK-886, and BAY x 1005.

The present invention still further relates to a pharmaceutical composition of the invention together with a receptor antagonist for leukotrienes LTB₄, LTC₄, LTD₄, and LTE₄ selected from the group consisting of the phenothiazin-3-ones such as L-651,392; amidino compounds such as CGS-25019c; benzoxalamines such as ontazolast; benzenecarboximidamides such as BIIL 284/260; and compounds such as zafirlukast, ablukast, montelukast, pranlukast, verlukast (MK-679), RG-12525, Ro-245913, iralukast (CGP 45715A), and BAY x 7195.

The present invention still further relates to a pharmaceutical composition of the invention together with a PDE4 inhibitor including inhibitors of the isoform PDE4D. The present invention still further relates to a pharmaceutical composition of the invention together with a antihistaminic Hi receptor antagonists including cetirizine, loratadine, desloratadine, fexofenadine, astemizole, azelastine, and chlorpheniramine.

The present invention still further relates to a pharmaceutical composition of the invention together with a gastroprotective H₂ receptor antagonist or the proton pump inhibitors (such as omeprazole)

The present invention still further relates to a pharmaceutical composition of the invention together with an 0 - and α₂-adrenoceptor agonist vasoconstrictor sympathomimetic agent, including propylhexedrine, phenylephrine, phenylpropanolamine, pseudoephedrine, naphazoline hydrochloride, oxymetazoline hydrochloride, tetrahydrozoline hydrochloride, xylometazoline hydrochloride, and ethylnorepinephrine hydrochloride.

The present invention still further relates to a pharmaceutical composition of the invention together with anticholinergic agents including ipratropium bromide; tiotropium bromide; oxitropium bromide; pirenzepine; and telenzepine.

The present invention still further relates to a pharmaceutical composition of the invention together with methylxanthanines including theophylline and aminophylline; sodium cromoglycate; or muscarinic receptor (Ml, M2, and M3) antagonist.

The present invention still further relates to a pharmaceutical composition of the invention together with a modulators of chemokine receptor function such as CCR1, CCR2, CCR2A, CCR2B, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CCR10 and CCR11 (for the C-C family); CXCR1, CXCR3, CXCR4 and CXCR5 (for the C-X-C family) and CX3CRI for the C-X3-C family. The present invention still further relates to a pharmaceutical composition of the invention together with an insulin-like growth factor type I (IGF-1) mimetic.

The present invention still further relates to a pharmaceutical composition of the invention together with (a) tryptase inhibitors; (b) platelet activating factor (PAF) antagonists; (c) interleukin converting enzyme (ICE) inhibitors; (d) IMPDH inhibitors; (e) adhesion molecule inhibitors including VLA-4 antagonists; (f) cathepsins; (g) glucose-6 phosphate dehydrogenase inhibitors; (h) kinin-Bi - and B₂ -receptor antagonists; (i) anti-gout agents, e.g., colchicine; (j) xanthine oxidase inhibitors, e.g., allopurinol; (k) uricosuric agents, e.g., probenecid, sulfinpyrazone, and benzbromarone; (1) growth hormone secretagogues; (m) transforming growth factor (TGFβ); (n) platelet-derived growth factor (PDGF); (o) fibroblast growth factor, e.g., basic fibroblast growth factor (bFGF); (p) granulocyte macrophage colony stimulating factor (GM-CSF); (q) capsaicin cream; (r) Tachykinin NKi and NK₃ receptor antagonists selected from the group consisting of NKP-608C; SB- 233412 (talnetant); and D-4418; and (s) elastase inhibitors selected from the group consisting of UT-77 and ZD-0892 (t) induced nitric oxide synthase inhibitors (iNOS) or (u) chemoattractant receptor-homologous molecule expressed on TH2 cells, (CRTH2 antagonists).

The pharmaceutical composition of the invention may also be used in combination with existing therapeutic agents for the treatment of osteoarthritis. Suitable agents to be used in combination include standard non-steroidal anti-inflammatory agents (hereinafter NS AID's) such as piroxicam, diclofenac, propionic acids such as naproxen, flubiprofen, fenoprofen, ketoprofen and ibuprofen, fenamates such as mefenamic acid, indomethacin, sulindac, apazone, pyrazolones such as phenylbutazone, salicylates such as aspirin, induced nitric oxide synthase inhibitors (iNOS inhibitors), and the cylco-oxygenase inhibiting nitric oxide donors (CINOD' s) analgesics (such as paracetamol and tramadol), cartilage sparing agents such as diacerein, doxycyline and glucosamine, and hyaluronic acids such as hyalgan and synvisc. The pharmaceutical composition of the invention may also be used in combination with existing therapeutic agents for the treatment of inflammatory bowel diseases (Ulcerative colitis and Crohn's disease). Suitable agents to be used include 5-amino-salicylates, the thiopurines, azathioprine and 6-mecaptorurine.

The pharmaceutical composition of the invention may also be used in combination with anticancer agents such as endostatin and angiostatin or cytotoxic drugs such as adriamycin, daunomycin, cis-platinum, etoposide, taxol, taxotere and farnesyl transferase inhibitors, VegF inhibitors, and antimetabolites such as antineoplastic agents, especially antimitotic drugs including the vinca alkaloids such as vinblastine and vincristine.

The pharmaceutical composition of the invention may also be used in combination with antiviral agents such as Viracept, AZT, aciclovir and famciclovir, and antisepsis compounds such as Valant.

The pharmaceutical composition of the invention may also be used in combination with calcium channel blockers, lipid lowering agents such as fibrates, beta-blockers, Ace inhibitors, Angiotensin-2 receptor antagonists and platelet aggregation inhibitors.

The pharmaceutical composition of the invention may also be used in combination with CNS agents such as antidepressants (such as sertraline), anti-Parkinsonian drugs (such as deprenyl, L-dopa, Requip, Mirapex, MAOB inhibitors such as selegine and rasagiline, comP inhibitors such as Tasmar, A-2 inhibitors, dopamine reuptake inhibitors, NMDA antagonists, Nicotine agonists, Dopamine agonists and inhibitors of neuronal nitric oxide synthase), and anti Alzheimer's drugs such as donepezil, tacrine, propentofylline or metryfonate.

The pharmaceutical composition of the invention may also be used in combination with osteoporosis agents such as roloxifene, droloxifene, lasofoxifene or fosomax and immunosuppressant agents such as FK-506, rapamycin, cyclosporine, and azathioprine. The present invention will now be further understood by reference to the following illustrative examples.

The following P2X₇ antagonists were employed in the examples :-

1. N-[2-Methyl-5-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-ylcarbonyI)phenyl]- tricyclo[3.3.1.1 _■ 3, '7- ]decane-l-acetamide, hydrochloride

P2X₇ antagonist 1. (N-[2-Methyl-5-(9-oxa-3,7-diazabicyclo[3.3.1]non-3- ylcarbonyl)phenyl]-tricyclo[3.3.1.1³'⁷]decane-l-acetamide, hydrochloride ) was prepared as follows.

a) 3-(4-Methyl-3-nitrobenzoyl)-7-(phenylmethyI)-9-oxa-3,7-diazabicyclo[3.3.1]nonane

Oxalyl chloride (9.6ml) in dichloromethane (30ml) was added dropwise over 45 minutes to an ice-cooled solution of 4-methyl-3-nitro-benzoic acid (lO.Og) in dichloromethane (320ml) containing DMF (0.1ml). The reaction mixture was stirred at room temperature for 1 hour then concentrated in vacuo. The acid chloride was taken into THF (320ml) and cooled in an ice-bath before adding N,N-diisopropylethylamine (38ml) then 3- (phenylmethyl)-9-oxa-3,7-diazabicyclo[3.3.1]nonane, dihydrochloride (16.0g) (prepared as described in WO 01/028992) portion wise. The reaction was stirred for 18 hours then diluted with ethyl acetate (600ml) and washed with water (2x200ml) and saturated sodium bicarbonate (aq) (3x150ml) then dried (MgSO₄), filtered and concentrated to afford the sub-titled compound (18.5g).

m/z = 382

b) 3-(3-Amino-4-methylbenzoyl)-7-(phenylmethyl)-9-oxa-3,7- diazabicyclo[3.3.1]nonane

Reduced iron powder (7.9g) was added over 15 minutes to a stirred solution of the product of step a) (18.0g) and ammonium chloride (7.5g) in ethanol/water (3:1, 320ml) at 70°C. The reaction mixture was heated at reflux for 2 hours then filtered and concentrated in vacuo. The residue was taken into ethyl acetate (400ml), washed with water (2x150ml) then the organic phase dried (MgSO₄) and concentrated in vacuo to afford the sub-title compound (14.5g).

m z = 352

c) N-[2-Methyl-5-[[7-(phenylmethyI)-9-oxa-3,7-diazabicyclo[3.3.1]non-3- yl]carbonyl]phenyI]-tricycIo[3.3.1.1³'⁷]decane-l-acetamide Prepared by the method of step a) using 1-adamantaneacetic acid and the product of step b). Recrystallisation (ethyl acetate) afforded the sub-title compound.

m/z 528 d) N-[2-Methyl-5-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-ylcarbonyl)phenyI]- tricyclo[3.3.1.1³'⁷]decane-l-acetamide, hydrochloride

4M HCI in 1,4-dioxane (8ml) was added to a solution of the product of step c) (13.0g) in ethyl acetate (300ml). The resulting precipitate was isolated by filtration then suspended in ethanol (300ml) and 5% palladium on carbon (1.2g) added. The reaction mixture was stirred under 3 atmospheres pressure of hydrogen for 36 hours. Methanol was then added under an atmosphere of nitrogen, then the catalyst removed by filtration and the filtrate concentrated in vacuo. Recrystallisation (isopropanol: methanol 25:1, 800ml) gave the title compound (9.1 g) .

m/z 438 (M+H)⁺

δ_H (400MHz, d₆-DMSO, Me₄Si, 90°C) 9.06 (1H, s), 7.64 (1H, s), 7.25 (1H, m), 7.19 (1H, m), 4.15 (2H, s), 3.96 (2H, d, 14Hz), 3.35-3.23 (6H, m), 2.26 (3H, s), 2.14 (2H, s), 1.96 (3H, br s), 1.69-1.62 (12H, m).

Example 1

Pharmacological analysis to determine the effect of sulfasalazine / P2X₇ antagonist combinations (without addition of a P2X₇ agonist).

Human peripheral blood monocytes were prepared from the blood of healthy human volunteers collected in EDTA blood tubes. Monocytes were isolated by serial gradient centrifugation and washing to produce a pure population of cells. Lipopolysacharide (LPS) was then added to the cell suspension in tissue culture and this was incubated for 4 - 12 hours at 37 degrees centigrade. Sulfasalazine and / or a P2X₇ antagonist or vehicle was then added to the cells. After incubation, samples of cell supernatants were transferred to a 96-well plate for subsequent cytokine and mediator measurements. The formation of inflammatory mediators was measured in the cell supematants by specific ELISA assays for the cytokines LL-1, IL-18, TNFα and for other mediators including PGE2, NO and matrix metalloproteinases (MMPs). The levels of mediators released in the presence of a P2X₇ receptor antagonist alone, or in the presence of sulfasalazine alone, or in the presence of a combination of a P2X receptor antagonist with sulfasalazine were determined. The effects of the antagonists / sulfasalazine alone and in combination were then compared. Statistically significant levels of inhibitory activity against a single mediator (IL-1 or TNFα) or on multiple mediators by P2X₇ antagonist / sulfasalazine combinations, in comparison to that achieved by either a P2X antagonist or sulfasalazine alone, is an indicator for increased efficacy in the treatment of disease.

Example 2

Pharmacological analysis to determine the effect of sulfasalazine / P2X₇ anatagonist combinations (with addition of a P2X agonist).

Human peripheral blood monocytes were prepared from the blood of healthy human volunteers collected in EDTA blood tubes. Monocytes were isolated by serial gradient centrifugation and washing to produce a pure population of cells. Lipopolysacharide (LPS) was then added to the cell suspension in tissue culture and this was incubated for 4 - 12 hours at 37 degrees centigrade. Test mixtures were then added followed by the addition of the P2X₇ receptor agonist BzATP. Test mixtures can comprise of vehicle as control, a P2X₇ receptor antagonist, or a combination of a P2X₇ receptor antagonist together with sulfasalazine. After incubation, samples of cell supematants were transferred to a 96-well plate for subsequent cytokine and mediator measurements. The formation of inflammatory mediators was measured in the cell supematants by specific ELISA assays for the cytokines EL- 1, EL- 18, TNFα and for other mediators including PGE2, NO and matrix metalloproteinases (MMPs). The levels of mediators released in the presence of a P2X₇ receptor antagonist alone, or in the presence of a combination of a P2X receptor antagonist with sulfasalazine were determined. The effects produced by a P2X₇ antagonist alone and in combination with sulfasalazine were then compared. Statistically significant levels of inhibitory activity against a single mediator (EL-1 or TNFα) or on multiple mediators by P2X₇ antagonist / sulfasalazine combinations in comparison to that achieved by a P2X₇ antagonist alone is an indicator for increased efficacy in the treatment of disease.

Example 3

Assessment of anti-inflammatory activity of sulfasalazine / P2X₇ anatagonist combinations in rat Streptococcal cell wall-induced arthritis. ¹

Streptococcal cell wall (SCW)-induced arthritis was induced in the left ankle of female Lewis rats. Animals were sensitised by intra-articular injection of 5 μg (in 20 μL) SCW (Lee Laboratories) into the left ankle. Ankle swelling was assessed 3 days after injection and non-responders (animals with no apparent ankle swelling) were rejected. Responding animals were randomly allocated to the test groups.

Arthritis was induced 21 days after sensitisation by intravenous (iv) injection of SCW (100 μg in 500 μL saline). Animals were monitored and assessed on a daily basis through to termination 6 days after induction. The rats were housed on sawdust and provided with food and water ad libitum.

Oral dosing suspensions were in 1% (w/v) methylcellulose in deionised water and were freshly prepared on a daily basis. Compounds were administered by oral (4 mL/kg) prophylactic dosing, commencing 1 day prior to induction of arthritis through to termination on day 6 post-induction. The P2X₇ antagonist 1, was dosed at 30mg/kg (bid) and the sulfasalazine dosed at 50 mg/kg (bid).

Ankle diameters were measured with vernier callipers on a daily basis from day -1. Mechanical thresholds were assessed using von Frey filaments on days -1, 1, 3 and 5. The filaments were applied in increasing weights to the ankle region on the footpad of both feet. The first filament to induce a withdrawal response was considered to be the threshold.

Effects on ankle swelling and mechanical threshold were calculated on an area under the curve (AUC) basis, as the sum of the differences from individual day -1 values. Data analysis was by one-way ANOVA followed by Dunnett's test (ankle diameter) or Dunn's test (von Frey threshold) on the raw data (GraphPad Instat).

The left hind limbs were taken and X-rays of the tibio-tarsal compartment examined and scored (blinded) for radiologically evident lesions. Tissues were then processed for histopathological assessment. Data analysis was by a non-parametric one-way analysis of variance (ANOVA), followed by Kruskal-Wallis post-test.

1. Experimental procedure based on that described by Carlson RP, Jacobsen PB; 'Comparison of adjuvant and streptococcal cell wall-induced arthritis in the rat' in Morgan DW, Marshall LA, editors; In Vivo Models of Inflammation. Basel: Birkhauser Verlag; 1999.

Claims

C L A I MS

1. A pharmaceutical composition comprising, in admixture, a first active ingredient which is a P2X7 receptor antagonist, and a second active ingredient which is 2-hydroxy-5- [[4-[(2-pyridinylamino)sulfonyl]phenyl]azo]benzoic acid or a pharmaceutically acceptable derivative thereof.

2. A composition according to claim 1, wherein the P2X7 receptor antagonist is an adamantyl derivative.

3. A composition according to claim 1 or claim 2, wherein the P2X7 receptor antagonist is a compound of formula

wherein m represents 1, 2 or 3; la each R independently represents a hydrogen or halogen atom;

A^a represents C(O)NH or NHC(O);

Ar represents a group

X represents a bond, an oxygen atom or a group CO, (CH2)i-₆, CH=, (CH2)ι-₆θ, O(CH₂)i.₆, O(CH₂)₂-₆O, O(CH₂)2-3θ(CH₂)ι.3, CR'(OH), (CH₂)ι-3θ(CH₂)i-₃, (CH₂)ι-3θ(CH₂)2.3θ, NR^5a, (CH₂)ι_₆NR⁵ , NR^5a(CH₂)_1-6, (CH₂)ι.₃NR^5a(CH₂)i-₃, O(CH₂)₂-6NR^5a, O(CH₂)2-3NR^5a(CH2)ι.₃, (CH₂)ι-3NR^5a(CH₂)₂-₃θ, NR^5a(CH₂)₂.₆O, s NR^5a(CH₂)2-3O(CH2)i-3, CONR^5a, NR^5&CO, S(O)_n, S(O)_nCH₂, CH₂S(O)_n, SO₂NR^5a or NR^5aSO_{2 ;} n is 0, 1 or 2;

R' represents a hydrogen atom or a -Cg alkyl group;

2a 3a one of R and R represents a halogen, cyano, nitro, amino, hydroxyl, or a group o selected from (i) C^-Cg alkyl optionally substituted by at least one C3-C6 cycloalkyl,

(ii) C3-C8 cycloalkyl, (iii) C -C(, alkyloxy optionally substituted by at least one

2a 3a substituted by one or more fluorine atoms, and the other of R and R represents a hydrogen or halogen atom;

4a s either R represents a 3- to 9-membered saturated or unsaturated aliphatic heterocyclic ring system containing one or two nitrogen atoms and optionally an oxygen atom, the heterocyclic ring system being optionally substituted by one or more substituents independently selected from fluorine atoms, hydroxyl, carboxyl, cyano, Cj-Cg alkyl,

Ci- hydroxyalkyl, -NR^6aR^7a, -(CH₂)_rNR^6aR^7a and -CONR^6aR^7a,

4a 0 or R represents a 3- to 8-membered saturated carbocyclic ring system substituted by one

6Σ _ 7a a^_ 7a or more substituents independently selected from -NR R , -(CH₂)_rNR R and . a -CONR K , the ring system being optionally further substituted by one or more substituents independently selected from fluorine atoms, hydroxyl and Ci-Cg alkyl; r is 1, 2, 3, 4, 5 or 6;

5a 5 R represents a hydrogen atom or a Cj-Cg alkyl or C3-C8 cycloalkyl group;

6a 7a

R and R each independently represent a hydrogen atom or a C\ -Cβ alkyl,

6a. 7a

C₂-Cβ hydroxyalkyl or C3-C8 cycloalkyl group, or R and R together with the nitrogen atom to which they are attached form a 3- to 8-membered saturated heterocyclic ring; 0 with the provisos that, (a) when A represents C(O)NH and R represents an unsubstituted 3- to 8-membered saturated aliphatic heterocyclic ring system containing one nitrogen atom, then X is other than a bond, and

(b) when A represents C(O)NH and X represents a group (CH2)i-₆ or O(CH₂)ι_₆, then

(c) when A represents NHC(O) and R represents an unsubstituted 3- to 8-membered saturated aliphatic heterocyclic ring system containing one nitrogen atom, then X is other than a bond, and (d) when A^a represents NHC(O) and X^a represents O(CH₂)i-₆, NH(CH₂)ι.₆ or SCH₂,

(e) when A^a represents NHC(O) and X^a represents O(CH2)₂-₃NH(CH2)₂, then R ^& does not represent an imidazolyl group; or a pharmaceutically acceptable salt or solvate thereof.

4. A composition according to claim 1 or claim 2, wherein the P2X7 receptor antagonist is a compound of formula

wherein D represents CH2 or CH₂CH₂; E^b represents C(O)NH or NHC(O);

1 K

R and R each independently represent a hydrogen or halogen atom, or an amino, nitro, Cj-Cg alkyl or trifluoromethyl group; R represents a group of formula

.4b ,5b

(HI);

X represents an oxygen or sulphur atom or a group NH, SO or SO ; ι_ 1 1 T-» Y represents an oxygen or sulphur atom or a group NR , SO or SO₂; Z represents a group -OH, -SH, -CO₂H, Cj-Cg alkoxy, Cι-C6 alkylthio, Ci-Cβ-alkylsulphinyl, Cι-C₆.alkylsulphonyl, -NR^6bR^7b, -C(O)NR^8bR^9b, imidazolyl,

10h

1-methylimidazolyl, -N(R )C(O)-Cι-C6 alkyl, -Co alkylcarbonyloxy, Cι-C₆ alkoxycarbonyloxy, -OC(O)NR^12bR^13b, -OCH₂OC(O)R^14b, -OCH₂OC(O)OR or -OC(O)OCH₂OR^16b;

4b R represents a C₂-C6 alkyl group;

R represents a Cj-Cg alkyl group;

_ 6b 7b ₀8b _,9b 10b _D12b , _,13b . . .

R , R , R , R , R , R and R each independently represent a hydrogen atom, or a Ci-C6 alkyl group optionally substituted by at least one hydroxyl group; R represents a hydrogen atom, or a -Cg alkyl group optionally substituted by at least one substituent independently selected from hydroxyl and Ci-Cg alkoxy; and

R , R and R each independently represent a Cj-Cg alkyl group; with the provisos that (i) when E represents NHC(O), X represents O, S or NH and Y b _^__ 6b_.7b _τ-.6b represents O, then Z represents -NR R where R represents a hydrogen atom and R represents either a hydrogen atom or a C -C alkyl group substituted by at least one b b hydroxyl group, and (ii) when E represents NHC(O), X represents O, S or NH, Y represents NH and R represents CH2CH2, then Z is not -OH or imidazolyl; or a pharmaceutically acceptable salt or solvate thereof.

5. A composition according to claim 1 or claim 2, wherein the P2X7 receptor antagonist is a compound of formula

C wherein D represents CH₂ or CH2CH2; E^C represents C(O)NH or NHC(O);

1 r_* lc*

R and R each independently represent hydrogen, halogen, amino, nitro, Cj-Cg alkyl lc 2c or trifluoromethyl, but R and R may not both simultaneously represent hydrogen;

3c R represents a group of formula

4c 5c

,R .R

(V);

,4c R represents a Ci-Cg alkyl group; c 13c

X represents an oxygen or sulphur atom or a group NR , SO or SO2;

5c 5c

R represents hydrogen, or R represents Cj-Cg alkyl or C2-C6 alkenyl, each of which may be optionally substituted by at least one substituent selected from halogen, hydroxyl, c 6c

(di)-Cι-C₆-alkylamino, -Y -R ,

a 5- or 6-membered heteroaromatic ring comprising from 1 to 4 heteroatoms independently selected from nitrogen, oxygen and sulphur which heteroaromatic ring may itself be optionally substituted by at least one substituent selected from halogen, hydroxyl and C_rC₆ alkyl; Y represents an oxygen or sulphur atom or a group NH, SO or SO2;

6c 7c c 7c c

R represents a group -R Z where R represents a C₂-Cg alkyl group and Z represents an -OH, -CO₂H, -NR⁸°R⁹ , -C(O)NR¹⁰°R^11C or -N(R^12c)C(O)-C_rC₆ alkyl group, and, c 6c in the case where Y represents an oxygen or sulphur atom or a group NH, R additionally represents hydrogen, Ci-Cg alkyl, Cχ-C6 alkylcarbonyl, Ci-Cό

1 c 1 r 1 fir 17r> alkoxycarbonyl, -C(O)NR R , -CH₂OC(O)R , -CH₂OC(O)OR or

-C(O)OCH₂OR^18c;

8c 9c 10c lie 12c R , R , R , R and R each independently represent a hydrogen atom or a Ci-Cg alkyl group;

13c 13c

R represents hydrogen, C3-C8 cycloalkyl, C3-C8 cycloalkylmethyl, or R represents a C^-Cg alkyl group optionally substituted by at least one substituent selected from hydroxyl and Ci -Cg alkoxy; and

„ 14c _ 15c „ 16c „ 17c , --.18c , . , , , „ „ „ , R , R , R , R and R each independently represent a -Cg alkyl group; with the proviso that when E° is C(O)NH, X° is O, NH or N(Cι-Cβ alkyl), then R⁵° is other than a hydrogen atom or an unsubstituted Ci-Cg alkyl group; or a pharmaceutically acceptable salt or solvate thereof.

6. A composition according to claim 1 or claim 2, wherein the P2X7 receptor antagonist is a compound of formula

wherein m represents 1, 2 or 3;

. Id each R independently represents a hydrogen or halogen atom; A^d represents C(O)NH or NHC(O);

Ar represents a group

(vπ) (vm) (DO

.2d .3d one of R and R represents halogen, nitro, amino, hydroxyl, or a group selected from (i) Ci-Cg alkyl optionally substituted by at least one halogen atom, (ii) C₃-C8 cycloalkyl, (iii) -Cg alkoxy optionally substituted by at least one halogen H "Ϊ atom, and (iv) C3-C8 cycloalkyloxy, and the other of R and R represents a hydrogen or halogen atom;

„4d

R represents a group

8d

X represents an oxygen or sulphur atom or a group >N-R n is 0 or 1 ;

R represents a C1-C5 alkyl group which may be optionally substituted by at least one substituent selected from hydroxyl, halogen and Cj-Cg alkoxy; ^j HA

R and R each independently represent a hydrogen atom, C₁- 3 alkyl (optionally substituted by at least one substituent selected from hydroxyl, halogen, Ci-Cg alkoxy, and (di)-Cj-C4 alkylamino (itself optionally substituted by at least one hydroxyl group)), or C₃-C₈ cycloalkyl (optionally substituted by at least one substituent selected from hydroxyl, halogen and Cj-Cfi alkoxy); and

8d R represents a hydrogen atom or a C1-C5 alkyl group which may be optionally substituted by at least one substituent selected from hydroxyl, halogen and Ci-Cό alkoxy; with the provisos that:

(d) when n is 0, then A is NHC(O), and

(e) whheenn nn iiss 1l,. XX rreepprreesseennttss ooxxyyggeenn aanndd A is C(O)NH, then R and R do not both simultaneously represent a hydrogen atom or do not both simultaneously represent an unsubstituted Cj-Cό alkyl, or when one of R and R represents a hydrogen atom, then the other of R and R does not represent an unsubstituted -Cfi alkyl; and (f) when n is l. X is oxygen, sulphur or >NH and A is NHC(O), then R and

HA

R do not both simultaneously represent a hydrogen atom or do not both simultaneously represent an unsubstituted Ci-Cfi alkyl, or when one of R

HA f^ HA and R represents a hydrogen atom, then the other of R and R does not represent an unsubstituted C -C alkyl or -CH₂CH₂OH; or a pharmaceutically acceptable salt or solvate thereof.

7. A composition according to claim 1 or claim 2, wherein the P2X7 receptor antagonist is a compound of formula

(XI) wherein m represents 1, 2 or 3; e

A represents C(O)NH or NHC(O);

,e

Y represents N or CH;

,e X represents a bond, CO, (CH₂)_1-6, O(CH₂)ι-6, (CH₂)_1-6NH(CH₂)ι_-6, (CH₂)_1-6O(CH₂)_1-6, NH(CH₂)_1-6;

Z^e represents NR^2eR^3e; le R represents halogen, cyano, nitro, amino, hydroxyl, Ci-Cg alkyl or C₃-C₈ cycloalkyl, which alkyl or cycloalkyl group group can be optionally substituted by one or more fluorine atoms; 2e 3e

R and R each independently represent a hydrogen atom, Cj-Cg alkyl or C3-C8 cycloalkyl, which alkyl or cycloalkyl group can be optionally substituted by one or more groups selected from hydroxyl, halogen or Ci-Cg alkoxy,

8. A composition according to claim 1 or claim 2, wherein the P2X7 receptor antagonist is:

2-Chloro-5-[[2-(2-hydroxy-ethylamino)-ethylamino]-methyl]-N- (tricyclo[3.3.1. l³'⁷]dec-l-ylmethyl)-benzamide,

(R)-2-Chloro-5-[3-[(2-hydroxy- 1 -methylethyl)amino]propyl]-N- (tricyclo [3.3.1.1³'⁷] dec- 1 -ylmethyl)-benzamide,

2-Chloro-5-[[2-[(2-hydroxyethyl)amino]ethoxy]methyl]-N-(tricyclo[3.3.1.1³'⁷]dec-l- ylmethyl)-benzamide, 2-Chloro-5-[3-[3-(methylamino)propoxy]propyl]-N-(tricyclo[3.3.1. l³'⁷]dec-l- ylmethyl)benzamide,

2-Chloro-5-[3-(3-hydroxy-propylamino)-propoxy]-N-(tricyclo[3.3.1. l³'⁷]dec-l- ylmethyl)-benzamide,

2-Chloro-5-[2-(3-hydroxypropylsulfonyl)ethoxy]-N-(tricyclo[3.3.1.1^3,7]dec-l- ylmethyl)-benzamide,

(tricyclo[3.3.1.1³'⁷]dec- 1 -ylmethyl)-benzamide,

2-Chloro-5-piperazin- 1 -ylmethyl-N-(tricyclo [3.3.1.1] dec- 1 -ylmethyl)-benzamide,

3 7 2-Chloro-5-(4-piperidinyloxy)-N-(tricyclo[3.3.1.1 ' ]dec- 1 -ylmethyl)-benzamide, 2-Chloro-5-(2,5-diazabicyclo[2.2.1]hept-2-ylmethyl)-N-(tricyclo[3.3.1.1]dec-l- ylmethyl)-benzamide,

37 2-Chloro-5-(piperidin-4-ylsulfinyl)-N-(tricyclo[3.3.1.1 ' ]dec-l-ylmethyl)-benzamide,

5-Chloro-2-[3-[(3-hydroxypropyl)amino]propyl]-N-(tricyclo[3.3.1. l³'⁷]dec-l- ylmethyl)-4-pyridinecarboxamide, 2-Chloro-5-[3-[[(lR)-2-hydroxy-l-methylethyl]amino]propyl]-N-

(tricyclo[3.3.1.1^3,7]dec- 1 -ylmethyl)-3-pyridinecarboxamide,

5-Chloro-2-[3-(ethylamino)ρropyl]-N-(tricyclo[3.3.1.1^3,7]dec-l-ylmethyl)-4- pyridinecarboxamide,

5-Chloro-2-[3-[(2-hydroxyethyl)amino]propyl]-N-(tricyclo[3.3.1.1³'⁷]dec-l-ylmethyl)- 4-pyridinecarboxamide,

5-Chloro-2-[3-[[(25)-2-hydroxypropyl]amino]propyl]-N-(tricyclo[3.3.1.1^3,7]dec-l- ylmethyl)-4-pyridinecarboxamide,

N-[2-Methyl-5-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-ylcarbonyl)phenyl]- tricyclo [3.3.1.1³'⁷] decane- 1 -acetamide, or a pharmaceutically acceptable salt or solvate of any one thereof.

9. A composition according to any one of claims 1 to 8, wherein the second active ingredient is 2-hydroxy-5-[[4-[(2-pyridinylamino)sulfonyl]phenyl]azo]benzoic acid.

10. A composition according to any one of claims 1 to 9 which is formulated for oral administration.

1 1. A process for the preparation of a pharmaceutical composition as defined in any one of claims 1 to 9 which comprises mixing the first active ingredient with the second active ingredient.

12. Use of a composition according to any one of claims 1 to 9 in the manufacture of a medicament for the treatment of an inflammatory disorder.

13. Use according to claim 12, wherein the inflammatory disorder is rheumatoid arthritis.

14. A method of treating an inflammatory disorder which comprises administering a therapeutically effective amount of a pharmaceutical composition as defined in any one of claims 1 to 9 to a patient in need thereof.

15. A method according to claim 14, wherein the inflammatory disorder is rheumatoid arthritis.

16. A pharmaceutical product comprising, in combination, a preparation of a first active ingredient which is a P2X7 receptor antagonist, and a preparation of a second active ingredient which is 2-hydroxy-5-[[4-[(2-pyridinylamino)sulfonyl]phenyl]azo]benzoic acid or a pharmaceutically acceptable derivative thereof, for simultaneous, sequential or separate use in therapy.

17. A kit comprising a preparation of a first active ingredient which is a P2X7 receptor antagonist, a preparation of a second active ingredient which is 2-hydroxy-5-[[4-[(2- pyridinylamino)sulfonyl]phenyl]azo]benzoic acid or a pharmaceutically acceptable derivative thereof, and instructions for the simultaneous, sequential or separate administration of the preparations to a patient in need thereof.