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WO2004035759A3 - Inhibitory oligonucleotides targeted to matrix metalloproteinase-9 - Google Patents

Inhibitory oligonucleotides targeted to matrix metalloproteinase-9 Download PDF

Info

Publication number
WO2004035759A3
WO2004035759A3 PCT/US2003/033141 US0333141W WO2004035759A3 WO 2004035759 A3 WO2004035759 A3 WO 2004035759A3 US 0333141 W US0333141 W US 0333141W WO 2004035759 A3 WO2004035759 A3 WO 2004035759A3
Authority
WO
WIPO (PCT)
Prior art keywords
matrix metalloproteinase
oligonucleotides
inhibitory oligonucleotides
oligonucleotides targeted
inhibitory
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/033141
Other languages
French (fr)
Other versions
WO2004035759A2 (en
Inventor
Duane E Ruffner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genta Salus LLC
Original Assignee
Genta Salus LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genta Salus LLC filed Critical Genta Salus LLC
Priority to AU2003287169A priority Critical patent/AU2003287169A1/en
Publication of WO2004035759A2 publication Critical patent/WO2004035759A2/en
Publication of WO2004035759A3 publication Critical patent/WO2004035759A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Inhibitory oligonucleotides are disclosed which are targeted to six specific target regions found on nucleic acids encoding matrix-metalloprotein-9. These inhibitory oligonucleotides are generally of from about 8 to about 50 nucleotides in length. Presently preferred oligonucleotides are disclosed. The oligonucleotides of the invention may be incorporated into compositions such as pharmaceutical compositions, and may additionally be used in methods of inhibiting the expression of matrix metalloproteinase-9 in a cell or tissue.
PCT/US2003/033141 2002-10-18 2003-10-17 Inhibitory oligonucleotides targeted to matrix metalloproteinase-9 Ceased WO2004035759A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003287169A AU2003287169A1 (en) 2002-10-18 2003-10-17 Inhibitory oligonucleotides targeted to matrix metalloproteinase-9

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27378302A 2002-10-18 2002-10-18
US10/273,783 2002-10-18

Publications (2)

Publication Number Publication Date
WO2004035759A2 WO2004035759A2 (en) 2004-04-29
WO2004035759A3 true WO2004035759A3 (en) 2005-03-31

Family

ID=32106462

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/033141 Ceased WO2004035759A2 (en) 2002-10-18 2003-10-17 Inhibitory oligonucleotides targeted to matrix metalloproteinase-9

Country Status (2)

Country Link
AU (1) AU2003287169A1 (en)
WO (1) WO2004035759A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999013886A1 (en) * 1997-09-17 1999-03-25 East Carolina University Multiple target hybridizing nucleic acids, their preparation, compositions, formulation, kits and applications

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999013886A1 (en) * 1997-09-17 1999-03-25 East Carolina University Multiple target hybridizing nucleic acids, their preparation, compositions, formulation, kits and applications

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BRANCH A.: "A good antisense molecule is hard to find", TIBS, vol. 23, 1998, pages 45 - 50, XP004108000, DOI: doi:10.1016/S0968-0004(97)01155-9 *
JEN ET AL.: "Suppression of gene expression by targeted disruption of messanger RNA: available options and current strategies", STEM CELLS, vol. 18, 2000, pages 307 - 319 *

Also Published As

Publication number Publication date
AU2003287169A1 (en) 2004-05-04
WO2004035759A2 (en) 2004-04-29
AU2003287169A8 (en) 2004-05-04

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