WO2004031736A1 - Burrless embedding pharmaceutical preparation plate for pathological tissue sample, embedding phamaceutical preparation frame, cassette, and embedding pharmaceutical preparation method - Google Patents

Abstract

A burrless embedding pharmaceutical preparation plate for a pathological tissue sample capable of preventing burrs of embedding pharmaceutical preparation agent from occurring in clearances produced between the peripheral edges of a pathological tissue sample cassette and the peripheral edges of the opening part (2a) of the embedding pharmaceutical preparation plate (59) when the embedding pharmaceutical preparation agent is filled in the pathological tissue sample cassette and the embedding pharmaceutical preparation plate in the state of the cassette placed on the peripheral edge upper end face (4) of the opening part (2a) of the embedding pharmaceutical preparation plate (59), wherein a watertight frame (14) for fitting watertight the embedding pharmaceutical preparation plate to the outer peripheral surface of the square pathological tissue sample cassette is provided to the peripheral edge outer side of the opening part (2a) of the embedding pharmaceutical preparation plate (59) so as to be formed integrally with the embedding pharmaceutical preparation plate (59) and to be projected in a height direction.

Description

 Description Deburring-free embedding dish, embedding frame, cassette, and embedding method for pathological tissue samples

 According to the present invention, in order for a clinician to make a diagnosis and treatment plan for a disease, a pathological tissue sample collected from a patient is sliced into a stained sample, and a pathological diagnosis is made by the pathologist. When creating an embedding block for the pathological sample used in the pathological tissue sample management system before returning the product, it relates to a burr-free embedding plate, embedding frame, cassette, and embedding method for the pathological tissue sample It is.

 Further, even when a cassette having a conventional shape other than the cassette for pathological tissue samples in which no burrs are generated in Japanese Patent Application No. 2000-1509 of the inventor's prior application is used, the pathological tissue sample is sliced. When forming an embedding block of a pathological tissue sample in a state before the embedding, a burr is not generated on the embedding block, the thickness of the section at the time of the thin section is made uniform, and the accuracy of the pathological examination is improved. In the conventional embedding work process, burrs are generated around the conventional cassette due to the structure of the embedding dish, and the burrs are removed with a knife or heat. Eliminating and streamlining the work of removing the sample and embedding the sample data surface printed around the cassette, which occurs during the deburring operation of the conventional cassette using this conventional method of embedding the embedded tray, Read sample data after making block Ru Der things to prevent it difficult to Ri. Background art

Conventionally, in this type of work, a clinician stores a histopathological tissue sample from each patient in a container with a label, etc., bearing the name of each patient or other data. They are sent together with the test request that describes the attributes and clinical diagnosis and findings, etc., and are transported by appropriate means such as mailing or messenger. The specimen sample of each patient stored in the above-mentioned container transferred to the pathological examination side at a location away from the clinician is accepted there before patient specimen preparation work for pathological diagnosis by the pathologist 'entry The pathological diagnosis test request form, which is attached together with the patient sample container at one time, is compared with the name of each patient, the contents of the request, the status of the pathological tissue sample, etc. Make sure it belongs to the patient.

 After that, at the time of the reception and entry, the clinician's patient ID number and chart number at the time of collection of histopathological tissue samples, as well as the pathological tissue sample collation random number at the time of patient sample collection / delivery or messenger sample reception. Unrelated, another cassette with the patient's sample number listed in a serial specification number or sample number (a microtome with a sample processing basket and sample embedding block frame base developed in America more than 30 years ago) For systematization that can be attached and detached with one touch, the size to fit into the adapter is about 40 x 28 x 6 marauder, and the locking claw of the adapter fits in the position of this foreign law All cassettes have this position.) The above-mentioned pathological sample of the patient, which has been trimmed, is stored in After processing, all samples are picked up with tweezers, etc., transferred to the bottom of the embedding dish, embedded with an embedding agent, and attached to a base made of a cassette frame-shaped substrate. The embedding cassette block sample is completed by removing and removing excess burrs of the embedding agent formed around the cassette frame-shaped substrate which is the base of the embedding cassette block formed after the solidification of the embedding cassette block.

 Further, the embedding method using the pathological tissue sample cassette without burrs and the embedding plate of the above-mentioned Japanese Patent Application No. 2000-110509, which is described in Japanese Patent Application No. Use the cassette bottom consisting of a frame-shaped substrate with an opening formed and a perforated support bottom plate projecting below the outer peripheral surface of the bottom with a smaller area than the outer peripheral surface. This will produce a burr-free embedded block sample.

However, many of the conventional cassettes do not have the above-mentioned porous support bottom plate protruding with an area smaller than the outer peripheral surface of the bottom surface. Most of the printing printers (numbers, etc.) are used according to their shapes.

 Of course, a print printer for the bottom of the cassette with a protruding porous support bottom plate can be manufactured, or the opening and the porous support bottom plate can be modified to suit the use of existing print printers. There is no burr-free embedding dish using a conventional cassette bottom that is compatible with print printers.

 The block sample is sliced, the section is stretched and attached to a slide glass, dried, then de-embedded (deparaffinized), stained and enclosed to prepare a stained slide specimen, which is then examined by a pathologist. The pathological diagnosis is made by comparing the sample of each patient with the request form data and the medical history data, and a pathological diagnosis report is made to the clinician.

 An object of the present invention is to provide a clinician with a procedure for collecting pathological tissue sample samples including biopsy samples collected from each patient from the time of collection to the completion of a pathological diagnosis report. Eliminates the danger of loss or intrusion of the sample, request form data, and past history data), and the risk of human error in replacement, and if such a mistake occurs, trouble can occur anywhere after that And clarify where the responsibility lies.

 In the above-mentioned work process, human work is shortened or omitted in order to eliminate troubles that can be a major problem that cannot be recovered by simple human error, and management and monitoring by automation and mechanization Is to increase the number of work steps that can be performed.

For this purpose, macro images and pathological tissue samples that can be observed and confirmed from outside the perforated dish through the window of the fixed transfer container or through the window of the force set, which have already been filed by the inventor, can be obtained. The patient identification data (patient attributes, patient ID, patient chart number, etc.) written and attached at the time of collection on the clinical side and the reception reference number on the pathology side collect the sample from the clinical patient and the clinical data. Receipt of pathological diagnosis test request data and receipt of pathological test sample from submission of pathological diagnosis request ・ Conveyance ・ Reception and cut-out sorting ・ Drug processing ・ Embedding ・ Specimen preparation ・ History search ・ Pathological diagnosis ■ Data management and storage ■ It must be managed and used in a unified manner throughout the entire work process until the end of issuance of the pathological report. For that purpose, in addition to the observation and confirmation of the macro image of the sample in the cassette, a means such as a sample data number or a bar code and a three-dimensional data code for securely associating each patient data with the cassette sample is required. However, with the conventional method of preparing an embedded block, the data writing surface was damaged during the deburring operation of the cassette base and mechanical automatic reading was not possible, so patient data from the clinical side was transferred to the pathological sample preparation side. It could not be introduced. This patient sample data also has to ensure that the written surface of the block sample data is protected.

 For that purpose, in the conventional burr-free embedding dish and its embedding method, an opening is formed on the upper side of the cassette, and it is protruded with a smaller area than the outer peripheral surface of the bottom of the frame base that is also the adapter fitting part The inner peripheral surface of the paris-free embedding dish and the inner peripheral wall of the embedding dish opening are formed at the intersection concave portion surface of the side surface of the porous support bottom plate thus formed and the outer peripheral surface of the bottom surface of the frame-shaped substrate wider than the porous support bottom plate. The bottom of the frame-shaped base, in which the porous support bottom plate protrudes with an area smaller than the bottom area on the surface of the crossing convex portion, and the inner periphery of the embedding dish are fitted in a watertight manner. It is a hindrance when printing on many cassette printers or embedding in conventional embedding dishes. Disclosure of the invention

 The present invention provides a pathological specimen in which a watertight frame 14 for integrally fitting the outer peripheral surface of a rectangular pathological specimen cassette in a watertight manner is provided outside the periphery of an opening of an embedding dish in the height direction. It is an embedding dish without burrs.

In addition, a cassette frame 21 having a cassette opening 21 formed on the upper side and a porous bottom of the cassette having a porous liquid passage port formed on the lower side, and a cassette frame-shaped base that is detachably attached to the opening of the cassette frame-shaped base. In the cassette for a pathological sample comprising a lid having a porous liquid passage port to be fitted, the outer peripheral surface of the outer wall of the frame-shaped substrate is buried without the burr of the pathological sample (FIGS. 10, 11, and 1). (2) The cassette frame, which is an engaging portion of the adapter, which has an embedding opening that fits tightly with the inner peripheral surface of the watertight frame of (1) and detaches the cassette frame base of the cassette to the microtome with one touch. Intersection between the outer peripheral surface of the substrate and the outside of the porous bottom The cassette frame-shaped base is hooked by the difference portion, and the height of the outer peripheral surface of the outer wall of the frame-shaped base and the outer periphery of the rectangular opening of the packaging frame are integrally projected in the height direction. The heights of the three watertight frames provided are almost the same, and the watertight upper end surface for watertight fitting with the outside of the porous bottom surface of the cassette frame base on the other side at the periphery of the square opening is facing upward. Or a watertight frame of the embedding frame having the same height as the outer peripheral surface of the outer wall of the frame-shaped base body is integrally provided on the four peripheral edges of the rectangular opening in the height direction, or Although the height of the inner peripheral surface of the watertight frame of the embedding frame is lower than the height of the outer peripheral surface of the outer wall of the cylindrical substrate, the thickness of the watertight frame at the opening of the embedding frame is determined by the thickness of the embedding agent. Thickness (about 0.5 mm) so that it does not accumulate on the outer peripheral surface of the embedding frame and the contact point of the watertight frame of the embedding frame. Or an embedding dish opening inclined from the inside to the outside at the tip of the watertight frame at the opening of the embedding frame, and an embedding frame bottom plate or This is a burr-free packaging frame in which a general-purpose embedding dish is laid and the inner space is used as an embedding dish to form an embedding block for a pathological sample.

 Further, in the burrs-free embedding plate, in the force-set frame-like base that fits water-tightly into the opening of the burrs-free embedment frame, four or three side walls of the outer peripheral surface of the cassette are outside the porous bottom surface of the cassette. On the other hand, this is a pathological tissue sample cassette with a taper that is not obstructive to install the cassette in a conventional adapter that can be attached to and detached from the microtome with a single touch, and that has a slightly obtuse angle from a right angle.

 Furthermore, in the embedding dish and the embedding method without burrs using a cassette frame-like substrate which fits tightly into the opening of the embedding frame, a window is formed in the porous bottom surface of the cassette frame-like substrate 11. This is a burring-free embedding method, characterized in that the embedding method is used. BRIEF DESCRIPTION OF THE FIGURES

 FIG. 1 (A) is a plan view of a conventional embedding dish. (B) is a cross-sectional view taken along the line IB-IB at the time of conventional embedding using the embedding dish of (A) of Fig. 1.

 FIG. 2 is a plan view of a burr-free embedding dish according to the invention of the applicant of the present invention.

(A) in Fig. 3 is a cross-sectional view taken along the line ΠΑ-ΉΙΑ in Fig. 2 and the frame base of the burr-free cassette. FIG. (B) is a cross-sectional view of a portion corresponding to the IE A-ffl A line portion of FIG. 2 of the cassette frame base without pallets and the embedding plate without burrs having shapes different from those of (A) of FIG. FIG. 4 is a cross-sectional view of the paris-free embedding dish, the burr-free cassette frame base, and the base lid of FIG.

 FIG. 5A is a perspective view of an embodiment of the embedding dish of the present invention. (B) is a perspective view of another embodiment in FIG. 5A.

 FIG. 6 is a plan view of FIG.

 (A) of FIG. 7 is a side view of the embodiment of the cassette frame-shaped base to be fitted with the embedding dish of FIGS.

 (B) is a side view of the embodiment of the cassette frame-shaped base to be fitted to the embedding dish different from (A) of FIG. 7. (C) is a side view of an embodiment of an embedding dish watertight frame and an embedding dish without Paris.

 (D) is a side view of an embodiment different from (C) of FIG. 7. (E) is a side view of an embodiment different from (D) of FIG. 7. (F) is a side view of an embodiment different from (E) of FIG. 7.

 (A) of FIG. 8 is a longitudinal sectional view of the cassette frame-shaped substrate of FIG. 7 (A). (B) is a longitudinal sectional view of an embodiment in which the cassette frame-shaped substrate of FIG. 8 (A) is fitted into an embedding dish. (C) is a longitudinal sectional view of a part of the embedding dish of FIG. 8 (B). (D) is a longitudinal sectional view of FIG. 7 (B). (E) is a longitudinal sectional view of the embodiment in which the part of FIG. 8 (D) is fitted into an embedding dish.

 (F) is a longitudinal sectional view of a part of FIG. 8 (E) and an enlarged view of the part.

 FIG. 9 (A) is a side view of another embodiment of FIG. 7 (A). (B) is a side view of the embedding dish into which the cassette of FIG. 9 (A) is fitted. (C) is a side view of the embodiment of the cassette frame-shaped base to be fitted to the embedding dish different from FIG. 9 (A). (D) is a side view of the embedding dish fitted with the force set of Fig. 9 (C). (E) is a plan view of FIG. 9 (D). FIG. 10 is a perspective view of an embedding frame without burrs and a bottom plate for an embedding frame according to an embodiment of the present invention different from those shown in FIGS. 5 (A) and 5 (B).

 FIG. 11 is a longitudinal sectional view showing an embodiment of the burr-free embedding frame of the present invention.

FIG. 12 is a cross-sectional view taken along line Χ-Π in FIG. (A) of FIG. 13 is a longitudinal sectional view of a cassette frame-shaped base of another embodiment. (B) is a longitudinal cross-sectional view of the flashless embedding frame fitted with the portion of FIG. 13 (A).

 (C) is a longitudinal sectional view of a general-purpose embedding dish for mounting the portion of FIG. 13B.

 FIG. 14 is a plan view when the parts of FIGS. 13 (A), 13 (B), and 13 (C) are fitted.

 FIG. 15 is a plan view of a cassette frame-shaped substrate showing another embodiment.

 FIG. 16 is a longitudinal sectional view taken along the line XVI-XVI of FIG.

 FIG. 17 is a longitudinal sectional view of the embodiment of the example showing a state in which the embedding dish cassette lid is closed by being fitted into the burr-free embedding frame of the portion of FIG.

 FIG. 18 is a cross-sectional view taken along the line XVI-XVI of FIG.

 FIG. 19 is a longitudinal sectional view of another embodiment different from FIG.

 FIG. 20 is a longitudinal sectional view of an embodiment of an embodiment showing a state of an embedding frame without burrs fitted with another box-shaped cassette frame base in the embodiment of FIG.

 FIG. 21 is a longitudinal sectional view showing the relationship between the frame-shaped substrate at the time of embedding when using the box-shaped cassette frame-shaped substrate of FIG. 20, the burrs without burrs, and the bottom plate for the burrs without burrs. It is. Figure 22 shows an example of a state in which a window is attached to the perforated bottom surface of a generally used conventional cassette frame base, fitted into a burr-free embedding frame, and the embedding dish cassette lid is closed. It is a longitudinal section of an embodiment.

 FIG. 23 is a cross-sectional view taken along the line Χ ΠΙ ΠΙ Χ Π 図 in FIG.

 FIG. 24 is a side view of each part of the embodiment of FIG.

 FIG. 25 is a plan view of each part of the example of FIG.

 FIG. 26 is an example of a back view of the embedding dish cassette lid which is one part of FIG. FIG. 27 is a side view of the form in which the parts of FIG. 24 are fitted and set.

 FIG. 28 is a plan view of FIG.

 Figure 29 is a plan view of an example of a triple general-purpose embedding dish that can be used when embedding an embedding frame without burrs.

Fig. 30 (A) shows a microtome adapter used when slicing a thin cassette frame-shaped substrate. It is sectional drawing which shows the relationship and shape of a thin cassette thickness adjustment plate for putters and a frame-shaped base | substrate. (B) is a sectional view showing another embodiment of a part of FIG. 3 OA.

 FIG. 31 (A) is a side view showing the relationship between the microtome adapter and the embedding cassette block. (B) is different from Fig. 31A. The relationship between the thin cassette thickness adjustment plate for the microtome adapter used for slicing the thin cassette frame base of Fig. 30 and the frame base, and the fitting state of the microtome adapter. It is a side view of the Example which shows the relationship and shape of this.

 FIG. 32 (A) is a perspective view showing the shape of the thin cassette frame-shaped substrate of the present applicant's earlier application. (B) is a slope view of another embodiment different from FIG. 32 (A). (C) is a perspective view of another embodiment different from FIG. 32 (B).

 FIG. 33 is an oblique view showing the relationship between the cassette frame-shaped substrate of FIG. 32 and the paris-free embedding frame.

 FIG. 34 (A) is a longitudinal sectional view showing the relationship between the cassette frame-shaped substrate of FIG. 33 and the burr-free embedding frame or the burr-free embedding plate. (B) is a sectional view of a burr-free embedding frame and a bottom plate for a burr-free embedding frame of another embodiment in a part of FIG. 34A.

 FIG. 35 is a longitudinal sectional view showing the embedding operation of FIG. 34 (A).

 FIG. 36 is a longitudinal sectional view showing the relationship between the burr-free embedding block cassette manufactured by the embedding operation of FIG. 35 and the thin cassette thickness adjusting plate.

 FIG. 37 is a perspective view of each part of FIG.

 FIG. 38 is a perspective view of another embodiment of FIG.

 FIG. 39 is a perspective view of the parts described in FIG.

 FIG. 40 is a longitudinal sectional view for explaining a cassette as the sample basket of FIG.

 FIG. 41 is a vertical cross-sectional view for explaining the use at the time of embedding in FIG.

 FIG. 42 is a side view showing the relationship between the thin cassette thickness adjusting plate of FIG. 36 and the cassette microtome adapter.

FIG. 43 (A) is a plan view showing an example of a fixed transfer plate (also used as a force setting cover). (B) is a sectional view taken along the line Χ Χ Χ ΙΠ 図 ΙΠ 図 の の in FIG. 43A. Figure 44 is an enlarged cross-sectional view of the fixed transfer plate of Figure 43B immersed in a transfer container containing the gelling fixative, and the relationship between the biopsy sample, the sample backing plate, and the embedded gel fixing support. FIG.

 FIG. 45 (A) is a diagram showing the use of the biopsy sample, the sample backing plate, the embedded gel-fixing support, and the positional relationship between the Π- Π maximum slice section.

 FIG. 46 (A) is a longitudinal sectional view of a schematic diagram of the embedding operation of FIGS. 44 and 45. (B) is a diagram showing a cross-sectional view of the embedding cassette block sample that can be obtained by embedding work and a positional relationship between the Π- Π maximum slice section and (B).

 FIG. 47 is a plan view of another embodiment of FIG. 43A.

 FIG. 48 is a perspective view of another embodiment of FIG.

 FIG. 49 is a diagram showing a comparison of the thickness of the thin burr-free embedding dish cassette of the present invention and a conventional cassette.

 FIG. 50 is a longitudinal sectional view of each part of another embodiment of the thin burr-free embedding dish cassette in comparison with FIG.

 FIG. 51 is a cross-sectional view showing a comparison of the thickness of a representative example of an embedding tray cassette without paris. FIG. 52 is a cross-sectional view showing a comparison of the thickness at the time of embedding of the embedding tray cassette without burrs. FIG. 53 is a cross-sectional view showing a comparison between the thickness of an embedded cassette block manufactured by using an embedding cassette cassette without burrs when the slice is stored and when it is stored.

 FIG. 54 is a perspective view showing a state before the pathological tissue sample cassette is fitted in a pathological tissue sample packaging dish in a watertight manner.

 FIG. 55 is a cross-sectional view taken along line XXXXXV-XXXXXV of FIG. 54 in a state after the pathological tissue sample cassette and the pathological tissue sample embedding dish shown in FIG. 54 are fitted in a watertight manner. BEST MODE FOR CARRYING OUT THE INVENTION

According to the present invention, a watertight frame 14 for watertight fitting with the outer surface 15 of a rectangular pathological sample cassette is integrally protruded in the height direction outside the periphery of the opening 2a of the embedding dish 59. Setting 7 is an embedding dish without burrs for a pathological sample.

 In addition, a rectangular watertight frame 14 is integrally protruded outside the peripheral edge of the opening 2a of the embedding dish 59 to fit tightly with the entire outer peripheral surface 1la of the rectangular pathological sample cassette. It is an embedding dish without a sample paris.

 In addition, a watertight frame 14 is integrally formed on the outer periphery of the rectangular opening 2a of the embedding dish 59 with the watertight frame 14 which is fitted watertightly with the outer peripheral surface 1la of the three sides of the rectangular disease sample cassette. At the same time, on the other side of the periphery of the rectangular opening 2a, a burrs-free embedding plate for pathological samples for upwardly forming a watertight upper end 4 for watertight fitting with the outer bottom surface 10b of the pathological sample cassette in a watertight manner. is there.

 Still further, the present invention provides the above-mentioned inventor, wherein the burr-free cassette frame-like substrate 11 according to the prior application of the present invention protrudes below the porous bottom outside 1 Ob with a smaller area than the porous bottom outside 1 Ob. The outer peripheral surface 13 a of the perforated support bottom plate 13 and the inner peripheral surface 7 of the embedding dish 7 are fitted in a watertight manner and the outer peripheral face 1 la of the burr-free cassette frame base 11 and the outer peripheral face of the embedding dish (8) was an embedding dish forming device for an embedding block, comprising an embedding dish almost identical to that of (8).

 However, in order to print barcodes, two-dimensional datacodes, sample numbers, patient names, and other patient data with many cassette automatic printing printers, the area is smaller than the outer peripheral surface of the bottom surface of the frame base of the cassette. The perforated indicator bottom plate that has been set is in the way.

 For this purpose, it is similar or almost similar to the cassette of the conventional shape, it can be set and printed on the automatic printing printer of the existing force set, and it can be embedded in all the conventional embedding trays. If an embedding dish that does not have burrs when embedding it in the frame-shaped substrate of the cassette with a dedicated embedding device—embedding dish is invented, it will be very convenient, and cost reduction and automation will be further advanced.

Furthermore, if the frame base of the cassette and the embedding device are also used in common, the embedding device can be integrated with a part of the cassette, thereby reducing the work process and cost, and further increasing automation. Therefore, troubles such as mistaking, mixing or loss of patient samples Disappears.

 For this purpose, a conventional burrs-free cassette frame-shaped substrate 11 is used to create a burrs-free embedded cassette block even if there is no porous support bottom plate protruding in a small area from the outer bottom 1 Ob of the porous bottom of 1 The outer peripheral surface 1 la of the frame-shaped substrate of the cassette frame-shaped substrate and the outer periphery 1 Ob of the porous bottom surface are used as a water-tight surface by reversing the uneven surface of the water-tight sealing surface of the substrate and the embedding dish.

 For this purpose, the height of the watertight frame 14 of the Paris-free embedding dish 59 is used as a means to take out the cassette frame-shaped substrate, which is the base of the embedded embedding block, from the burr-free embedding dish. This is to make the outer peripheral surface of the outer wall of the frame base lower than 1 la.

 Alternatively, as a means for taking out the cassette frame-shaped substrate which is an embedding block embedded from the paris-free packaging plate, the watertight frame 14 on one side of the burr-free embedded plate 59 is removed and the cassette frame-shaped substrate is removed. It is to be a take-out part.

 Alternatively, as a means to remove the cassette frame-shaped substrate, which is an embedded embedding block, from the burr-free embedding dish, remove the bottom surface of the burr-free embedding dish 59 and remove the bottom of the paris that is not a dish with a watertight frame 14. No embedding frame 19 is molded and the bottom is used with the plate attached to the bottom when embedding. Of course, the bottom can be easily removed, so the embedded cassette block can be pushed out from below.

 In addition, if the bottom of the burr-free embedding dish 59 is eliminated and the burr-free embedding frame 19 is formed, there is no bottom above and below the cassette frame-shaped substrate in which the burr is stored, which obstructs the passage of the chemical solution. Since there is only a frame around the cassette frame-shaped substrate 11 and the burr-free embedding frame 19 from the beginning, the role of the reagent processing basket, the role of the embedding plate and the block base as a block base The functions of the three roles are combined into one, and the work steps can be shortened, the sample can be prevented from being lost and mixed, and the cassette frame-shaped substrate 11 can be made thinner.

If the cassette frame-shaped substrate 11 is stored in the burial-free embedding frame 19 from the beginning and can be used as an integral embedding dish cassette, the embedding operation can be performed without removing the frame-shaped substrate 11. A window 22 is formed on the porous bottom surface 10 of the frame-shaped substrate 11. You.

 On top of that, if there is a window 22 on the porous bottom surface 10 of the cassette frame-like substrate 11 housed in the embedding frame 19 from the beginning, embedding work can be performed without removing the cassette frame-like substrate. Therefore, if these pathological tissue treatment force setting instruments are used together with the embedding gel fixing support material 46 which the applicant has already applied for a patent, the embedding operation can be automated.

 As shown in FIGS. 1 (A) and 1 (B), a conventional cassette frame-shaped substrate 11 is placed on a support 2 having no watertight surface of an opening 2a of the embedding dish, and an embedding agent 32 is placed on the support. In order to produce an embedding block by pouring into the porous bottom surface 10 of the conventional cassette, burrs 57 are generated around the cassette frame-shaped substrate 11 of the block sample.

 On the other hand, in the embedding dishes of FIGS. 2 to 4, the porous support bottom plate 13 is projected downward with a smaller area than the porous bottom outer surface 1 Ob of the other conventional frame-shaped base material 11, The concave surface of the outer peripheral surface 13 a of the porous support bottom plate 13 and the outer surface of the cassette frame-shaped substrate 11 a of the outer surface 1 1 Ob, the inner peripheral surface 7 of the embedding dish without burrs 7 and the upper end surface 4 No burrs 57 occur around the cassette of the block sample due to the watertight fit with the convex two-sided surface of the block.

 However, it is obvious that the same effect can be obtained even if the watertight surface is fitted watertight between the convex diagonal surface on the cassette side and the concave diagonal surface of the paris-free embedding dish.

 The inventor of the present invention made an embedding dish 59 having a shape as shown in FIGS. 5 to 9 and made the multi-hole bottom surface of the cassette frame-shaped substrate 11 of FIGS. 2 to 4 already filed by the inventor. Even if there is no porous support bottom plate 13 protruding downward with an area smaller than the outside 1 Ob and the conventional cassette shape, the shape of the watertight fitting part of the embedding dish without paris is the same, although the shape is reversed. We focused on the effect that can be obtained.

The shape of the cassette frame-shaped substrate 11 can be variously manufactured. As shown in FIGS. 3 to 10, not only a conventional trapezoid or box but also an inverted trapezoid (right in FIG. 3) can be embedded without paris. However, in the case of this embedding dish, the take-out part 16 to 16a of the frame 11 of the cassette is necessary in order to easily take out the embedding block from the burr-free embedding dish. It becomes important.

 Therefore, as shown in FIGS. 10 to 13, the storage bottom 3 of the burr-free embedding dish 59 shown in FIGS. 5 to 6 and 9 is removed, and the upper opening 2 a is also opened to the lower embedding frame opening bottom 18. A cylindrical burrs-free embedment frame 19 having a lower embedment frame open bottom 18 opened from the upper and lower openings 2a without any closed surface, is formed into the cassette frame-shaped base. 1 1 Lower embedding frame Open bottom 18 The outer peripheral surface of the cassette frame-shaped substrate, which is narrower than the 8 side, is narrowed down to an area slightly smaller than the entire bottom surface 10 of the frame-shaped substrate surrounded by 1 la. It has an embedding dish upper end face 4 shaped so that the periphery of the base 11 does not fall and falls down, and is formed to be the same or narrowed from the embedding storage section 20 below it to the embedding frame open bottom face 18 Then, a reference mark 5 which is left-right or vertically asymmetric is formed at one end of the open bottom portion 18 of the final lower embedding frame, and the existing large mark is formed on the lower surface thereof. Embedding dish or general-purpose embedding dish 5 4 or a thin plate, a bottom plate for burial-free embedding frame 29, is laid and sealed, and the embedding agent (32) is injected and placed on the cold plate. Then, when the embedding agent such as paraffin is solidified and solidified, a flashless embedding cassette block sample can be prepared.

 Furthermore, if an embedding block sample is prepared using the paris-free embedding frame 19 of this shape, the force-setting frame-shaped substrate 11 can be made water-tight on all four sides so that burrs are not completely attached, and the embedding frame is Unlike the filling plate, it has no bottom and is open, so if you push the bottom upward, you can easily remove the cassette block sample without burrs. The plan view of the embedding is shown in Fig. 14, and the embedding agent flowing from the porous bottom surface 10 of the cassette frame base 11

3 Pour 2 in.

Further, as shown in FIGS. 15 to 18, a window 22 is formed in the porous bottom portion 10 of the cassette frame-shaped substrate, and small samples such as a biopsy sample are separately stored around the window 22. When the small room 2 3 for partitioning is formed by partitioning the partition wall 24, the cassette frame-shaped substrate 11 is inserted into the burial-free embedment frame 19 while the existing large-sized lower surface of the embedment frame 19 is inserted. Embedding dish or general-purpose embedding dish 54 or thin section on bottom plate for embedding frame 29 without burrs A block sample can be made.

 Furthermore, as shown in FIGS. 17 to 31, the cassette frame-shaped substrate 11 is previously mounted in the packaging frame without burrs, and the cassette opening 21 of the frame-shaped substrate and the burrs are not mounted. Then, as shown in FIG. 17 or FIG. 18 and FIGS. 22 to 28, the watertight frame tip portion 14 e of the embedding frame 19 is closed together with the embedding tray cassette lid 26, and the embedding frame is closed. 19 Embedding frame open When the bottom 18 is closed with the burr-free embedding frame open bottom side lid 27 as shown in Fig. 19 or Fig. 20 and Fig. 50 and Fig. 51 (B), the embedding frame The sample in the cassette frame-shaped substrate together with the sample 19 can be set in an automatic fixed embedding device, and the chemical treatment of dehydration, degreasing, and penetration can be performed. At this time, it is convenient to use a cassette bottom in which the window 22 is not formed in the porous bottom surface 10 of the frame-shaped base 11. It is not always the case that the surgical extirpated material has a fixed tissue shape, but there are also extirpated materials (fragment samples) that extract the tissue of the affected area, and these are large cassettes like biopsy samples. There is a high risk of flowing out of the through-hole, and it may be necessary to process a larger amount of tissue pieces than the biopsy sample.

 For such a case, as shown in FIGS. 19 to 20, a cassette frame-like substrate 11 having a porous bottom surface 10 having a small porous passage surface 10 without a window 22 is formed. You can put it.

 Furthermore, as shown in FIGS. 32 to 42, when the inventor applies the shape of the cassette frame-shaped substrate having a patent registration already, the embedding cassette block sample is obtained. The thickness can be made thinner.

When the embedded cassette block sample is sliced, the thin cassette thickness adjusting plate 33a for the U-shaped microtome adapter is attached to the protruding rim 40 of the cassette frame base as shown in FIGS. 37 and 42. Set to secure the size of the conventional cassette ⁴⁰ × 28 × 6 mm.

Further, as shown in FIGS. 38 to 41, the ridge 40 of the cassette frame-shaped base 11 is formed in the same rectangular size as the lower edge 41 of the projecting container. Then, the cassette lid and the perforated liquid flowing surface 60 When the cassette lid and burr-free embedding frame 19 b with the bottom plate insertion hole for burr-free embedding frame near the inner side 4 2 are formed, the cassette lid and paris-less embedding frame that is the lid after the sample is treated with the chemical solution Insert the bottom plate for burial-free embedding frame 4 3 from the insertion port 4 2 of the bottom frame for burrs without burrs without discarding 1 9 b. When used as an embedding dish when fitted to the lower edge 41 of the container, a flash-free embedded cassette block sample can be obtained.

 At this time, the operation of inserting and removing the bottom plate 43 for the burial-free embedding frame for insertion is performed by holding the take-out handle portion 45. In addition, a reference mark 5 is formed at the inner corner of the inside of the cassette lid and paris-free packaging frame 19 b, and a fiducial mark is formed on the bottom plate 4 3 for the paris-less embedding frame for insertion. Since the notches 44 are formed, the embedding block embedded by this embedding method can produce an embedding block with the fiducial mark 5 without burrs.

 Of course, the thin cassette thickness adjusting plate 33a for the U-shaped microtome adapter can be used even when the embedded cassette block is sliced.

 Furthermore, the present invention prevents the generation and adhesion of paris by fitting the convex diagonal surface of the cassette frame-shaped base of all shapes and the concave diagonal surface of the paris-free embedding dish in a watertight manner. Burr-free embedding can be performed at any position as long as there is a diagonal surface on the substrate. Therefore, the thickness of the cassette frame-shaped substrate can also be changed, and the thickness of the cassette frame-shaped substrate 11 and the burr-free embedding frame 19 shown in FIGS. The entire thickness of the frame-shaped substrate 11 set and fitted to the burrs-free embedding frame 19 is molded to the same thickness as that of the conventional cassette frame-shaped substrate, and the embedding function is retained in that part. The embedding dish cassette (Fig. 49-Fig. 53) was realized.

At this time, as shown in FIG. 12, the cassette frame-like base body 11 is provided with no burr-free embedding frame 19 on the outer surface of the cassette frame-like base body 19 so as not to be in the way when the microtome adapter is fitted. When the same taper 14 c as the inner peripheral surface 14 a of the frame is attached, the completed flash-free embedded cassette block is easily removed and removed. The embedding tray retainer 26 d of the embedding tray cassette lid 26 of FIGS. 23 to 28 is provided for holding the embedding frame 19 in the lateral direction. Furthermore, as shown in FIGS. 43 to 48 and FIGS. 52 to 53, a biopsy sample was prepared using the embedded gel-fixing support 46 already applied for by the applicant. 4) Biopsy sample in fixed cross-sectional view of fixed transfer plate 55 in Fig. 44 immersed in a gelling fixative such as alcohol formalin using fixed transfer plate 55 When stored in the order of collection, the biopsy samples are arranged in a line along the V-shaped structure of the biopsy sample V-shaped valley storage location 4-7 in Fig. 4-4. Inspection surface) can be obtained.

 This is simpler and more accurate than the work of preparing a pathological specimen by lifting a small biopsy sample in the embedding medium with tweezers or the like at the time of embedding to obtain the maximum sliced inspection surface.

 After arranging the biopsy samples, a small amount of the embedding gel-fixing support 46 was poured from above the V-shaped valley. A plurality of biopsy samples held by a small amount of embedded gel-fixed support material 46 gelled on one side of the backing paper, on which the sample position number is described, are collected into one sample.

 By using the fixed transfer plate 55 and the embedded gel fixing support 46 in this way, a plurality of biopsy samples are collectively held and the force setting frame accommodated in the burr-free embedding frame 19 is provided. The embedding operation can be completed without removing the cassette frame-shaped base 11 through the window 22 formed in the base 11.

 This means that the use of the embedded gel-fixed support 46, the (biopsy sample) fixed transfer plate 55, and the burr-free embedding dish cassette increases the possibility of automation of the embedding operation, If a solution is available in the mechanical field, it can be realized.

More specifically, Fig. 43 (A) is a fixed transfer plate 55 (biopsy sample) also used as a cassette lid. Fig. 43 (B) is a cross-sectional view of the XXXXBI-XXXXDI. 4 5 is a cross-sectional view and a plan view of the sample backing plate 49 and the embedded gel-fixing support material 46, which are part of the sample backing plate.Figure 46 shows the sample backing plate 49 and the embedded gel-fixing support material 4. 6 and the sample Fig. 47 and Fig. 48 are examples of other shapes of the (biopsy sample) fixed transfer plate 55.

 Further, in FIGS. 43 to 46, the biopsy sample 31 is arranged in the order of the position number 52 in the biopsy sample V-shaped valley storage location 47, and the embedded gel fixing support 46 is poured there. When the biopsy sample 31 is fixedly held on the sample backing plate 49, the biopsy sample 31 fits over the V-shaped valley and is fit regardless of the diameter of the biopsy sample 31. The centers of the test samples 31 are aligned on the same straight line.

 As a result, the biopsy sample 31 bundled with the embedded gel-fixed support 46 arranged in the V-shaped valley storage location 47 wraps the sample-embedded thin section 50. When embedded and mounted on the bottom plate 3 or the bottom plate 29 for burr-free embedding frames, the biopsy sample 31 in all the embedded gel-fixed supports is shown in Figures 44 to 46. The largest area Π— す る to be cut together will be sliced together.

 The sample transfer plate 55 of the fixed transfer plate 55 immersed in a fixing solution such as alcohol formalin shown in FIGS. 43 and 44 is preferably made of cardboard or a porous plate such as a polyvinyl alcohol plate. In particular, patient data can be described using a polyvinyl alcohol-based sample backing plate, and the biopsy sample placed and fixed on the sample backing plate with tweezers 51 in Fig. 44 is transferred to the pathology laboratory for the fixed sample 31. It can also be used for simple fixation and lining on the clinical side (sample backing point 56) before being performed, and the biopsy that has been easily placed on the sample backing on the clinical side and fixed and transferred When the sample is cut out in the pathological examination room, the biopsy sample can be transferred to the V-shaped valley storage location 47 after confirming and observing again in the order of position number 52, and rearranged and sliced. Therefore, it can be embedded as it is as shown in Fig. 45 and Fig. 46.

FIGS. 47 and 48 show another embodiment of the fixed transfer plate 55.

Although the present invention has been described with respect to the above-described embodiment, the present invention is not limited to the embodiment, and specific technical means for more effectively implementing the embodiment may be added, or the embodiment may be modified. Can be partially changed. For example, when the above-mentioned burrs-free embedding dish cassette is used, as shown in FIG. 46A, the cassette frame-shaped substrate 11 is not removed from the burrs-free embedding frame 19, and the lid of the embedding dish cassette is removed. After removing only 26, the patient backing plate 49 with the patient name data and sample position number 52 in the cassette frame base 11 shown in FIG. A sample 31 in which a test sample is bundled with an embedding gel-fixing support material 46 is used for the general purpose in which the embedding frame 19 is placed from the window 22 of the frame-shaped base 11 of the paris-free embedding dish cassette 1 1. If the sample is transferred onto an embedding dish 54 or a bottom plate for embedding frame 29 without burrs and embedded with an embedding agent 32, mixing and replacement of the sample is completely eliminated, and multiple samples as shown in Fig. 29 are obtained. Simultaneous embedding is possible with a series of general-purpose embedding dishes 54a, and the embedding work becomes more efficient.

 In addition, as shown in FIGS. 54 and 55, in the cassette frame-shaped base 11 that fits water-tightly into the opening 2 a of the burr-free embedding dish 59 Hanging on the opposing peripheral surface 1 la of the cassette frame-shaped substrate 11 1, the cassette positioning stopper projection 61 formed on the inner peripheral surface of the burr-free embedding dish 59 and the burr-free embedding frame 19. By forming the cassette positioning stopper recess 1 le that is removably fitted, the cassette frame-shaped base 11 can be easily and accurately formed into the burr-free embedding dish 59 or the burr-free packaging frame 19. It is possible to fit the opening 2a in a watertight manner. Industrial applicability

As described above, the present invention provides a convex diagonal surface or one surface where the outer peripheral surface of the cassette frame-shaped base intersects with the bottom and a concave diagonal surface or one surface where the watertight frame of the burr-free embedding plate intersects with the upper end surface of the embedding plate. In this way, the cassette frame-shaped base is fitted in a watertight frame on the upper end surface of the embedding dish so that the embedding agent on the bottom of the embedding dish does not overflow onto the outer peripheral surface of the cassette frame-shaped base. However, in the case of the burial-free embedment frame, which is an advanced form of the embedding work, even if the height of the watertight frame around the entire circumference is the same as the height of the outer peripheral surface of the cassette frame-shaped substrate, that is, the thickness of the frame-shaped substrate. The completed embedding block cassette is placed above the open bottom of the embedding frame of the frame-shaped substrate. It can be easily removed by pushing it out, so there is no need for a cassette removal unit such as an embedding dish without burrs. .

 Therefore, as shown in Fig. 17 and Fig. 20 and Fig. 38, Fig. 50, and Fig. 51, the gap between the bottom of the porous frame base of the cassette and the open bottom side lid of the paris-free embedding frame, When a window is formed on the bottom, there is a space completely surrounded by a porous surface between the lid of the embedding dish cassette and the lid on the bottom of the embedding frame without burrs. A cassette with an embedding function incorporated as an embedding dish cassette is completed. Use the burr-free embedding dish and the burr-free embedding frame without the paris! / In that case, it becomes possible to make the thickness of the cassette frame-shaped substrate thinner. Especially, when using an embedded dish cassette, a large sample that is surgically extracted, cut out and trimmed from a small sample such as a biopsy sample Depending on the size of the sample up to the sample, small samples are stored in a thin cassette frame-shaped substrate, and large samples are stored and fixed in the sample storage section of the paris-less embedded frame. It can be used for chemical treatment of the filling device.

 In the embedding dish cassette of the present invention, since the cassette frame-shaped substrate is mounted on the embedding frame from the beginning, there is no need to select or mount the embedding dish when embedding, as shown in FIG. Place the embedding dish cassette on the bottom plate (or general-purpose embedding dish), open the embedding dish cassette lid, and place a thin section of the sample through the window on the porous bottom. All you have to do is put the liquid embedding medium on top and cool it.

 Also, since the cassette frame-shaped substrate can be made thin as shown in Figs. 31 and 42 and Figs. 51 to 53, it is set on a microtome adapter by laying it on a thickness adjustment plate when slicing. When the block sample is stored and stored, since the block cassette sample is thinner than the conventional cassette frame-shaped substrate, the block cassette sample is also thin and requires less storage space.

 Further, as shown in FIGS. 22 to 28, the shape of the cassette frame-shaped substrate can be formed in accordance with the conventional shape, so that it can be used in a conventional cassette printing printer.

In addition, when used together with the embedded gel fixing support and the fixed transfer plate, As shown in Fig. 3, it was possible to embed multiple biopsy samples as one sample without touching any samples, and it became possible to automate the embedding of small samples. Of course, if the patient's name is written on the fixed transfer plate, later confirmation can be assured.

Claims

The scope of the claims 1. A watertight frame (14) is fitted outside the periphery of the opening (2a) of the embedding dish (59) to fit tightly with the outer peripheral surface (1 la) of the square pathological sample cassette. A paris-free embedding dish for pathological tissue samples, characterized by being protruded in the vertical direction. 2. Outside the periphery of the opening (2a) of the embedding dish (59), a square watertight frame (11a) for watertight fitting with the entire outer peripheral surface (11a) of the square pathology sample cassette. A burr-free embedding dish for pathological tissue samples, characterized in that 14) is integrally protruded in the height direction. 3. The pathological tissue sample according to claim 1, wherein the height (h) of the watertight frame (14) is lower than the height (H) of the outer peripheral surface of the pathological sample cassette. No burrs Embedded dish. 4. A watertight frame (1 4) that fits tightly with the outer peripheral surface (1 la) of the rectangular pathological sample cassette on three sides on the outer periphery of the square opening (2a) of the embedding dish (59). ) As a whole in the height direction,  A watertight upper end surface (4) for watertightly fitting with the outer bottom surface (10b) of the porous sample of the pathological sample cassette is formed upward on one of the remaining portions at the periphery of the rectangular opening (2a). Burr-free embedding dish for pathological tissue samples. 5. The upper edge (4) engaging with the lower edge of the outer peripheral surface (1 1a) of the square sample cassette is formed around the opening (2a) of the embedding dish (59). Claim 1, 2. A flashless embedding dish for a pathological tissue sample according to 2, 3, or 4. 6. A watertight frame (14) that fits tightly with the outer peripheral surface (11a) of the rectangular pathological specimen cassette is integrated outside the periphery of the opening (2a) of the embedding dish (59). Protruding from the opening Place the open bottom (18) of the cylindrical embedding frame (19) with the mouth to bottom open on the top surface of the embedding frame bottom plate (29) such as a general-purpose embedding dish (54) in a watertight manner. A burrs-free embedding dish for pathological tissue samples, wherein the embedding space formed therein is embedded as a burr-free embedding dish. 7. An opening (21) is formed on the upper side, a porous frame (10) is formed on the lower side, and a force-setting frame base (11). 21) A lid (58) having a porous liquid inlet (58a) removably fitted to the diseased tissue sample cassette.  The outer peripheral surface (1 la) of the outer wall of the cassette frame-shaped base (11) is fitted in a watertight manner with the inner peripheral surface (14a) of the watertight frame of the embedding dish (59) for the pathological sample,  An intersecting portion (1 lc) between the outer peripheral surface (1 la) of the cassette and the outer surface of the porous bottom surface (1 Ob), which is a hook portion of an adapter for attaching and detaching the cassette frame-shaped substrate (11) of the cassette to the microtome with one touch, is provided. Into a shape that hooks the cassette, and  The height of the inner peripheral surface (14a) of the watertight frame of the embedding dish (59) is lower than the height of the outer peripheral surface (1 la) of the outer wall of the frame-shaped substrate (11),  The thickness (t) of the watertight frame (14) at the opening (2a) of the embedding dish (59) is such that the embedding agent (32) has the outer peripheral surface (1) of the outer wall of the frame-shaped substrate (11). la) and a force to make the contact point (14e) of the watertight frame (14g) of the embedding dish (59) so that it does not accumulate so much at the contact tip (14e), or the opening (2a) of the embedding dish (59) The embedding tray opening (2a) with a downward slope from the inside to the outside at the point (14e) of the watertight frame (14) of (1) above, which is free of Paris for pathological tissue samples. dish. 8. A frame-shaped substrate (11) having an opening (21) formed on the upper side and a bottom surface (10) having a porous liquid passage port below the opening (21); An embedding dish for a pathological sample cassette, comprising a lid (58) having a porous liquid passage port (58a) removably fitted into the opening (21); The outer peripheral surface (11a) of the three-side outer wall on the outside (1 Ob) of the rectangular porous bottom surface of the frame-shaped substrate (11) is connected to the inner peripheral surface (14a) of the three-way watertight frame of the embedding dish (59) for the pathological sample. ) And the cassette frame base (1 1), which is a hook for an adapter that allows the cassette frame base (1 1) to be attached to and detached from the microtome with a single touch, with the outer peripheral surface (1 la) of the cassette frame base (1 1). The bottom of the cassette frame-shaped base (11) is hooked to the intersection (1lc) of the bottom (1 Ob) and the outer peripheral surface (1 la) of the three-way outer wall of the cassette frame-shaped base (11). ) And the height (h) of the inner peripheral surface (14a) of the three-way watertight frame of the embedding dish (59) almost coincides with the embedding dish opening (2a),  The remaining outer peripheral surface (1 la) of one side of the cassette frame base body (11) and the one side watertight frame (14) of the embedding dish (59) have the form features and functions described in claim 7. A burr-free embedding dish for pathological tissue samples, characterized in that it has: 9. A frame-shaped substrate (11) having an opening (21) formed on the upper side and a bottom surface (10) having a porous liquid passage port below the opening (21); ) With a lid (58) having a porous liquid inlet (58a) that is detachably fitted into the opening (21) of the pathological tissue sample cassette. In  The outer peripheral surface (11a) of the three-side outer wall on the outside (1 Ob) of the rectangular porous bottom surface of the frame-shaped substrate (11) is connected to the inner peripheral surface (14a) of the three-way watertight frame of the embedding dish (59) for the pathological sample. ) And the cassette frame base (1 1), which is a hook for an adapter that allows the cassette frame base (1 1) to be attached to and detached from the microtome with a single touch, with the outer peripheral surface (1 la) of the cassette frame base (1 1). The shape is such that the cassette frame base (1 1) is hooked at the intersection (1 lc) of the bottom (1 Ob) on the bottom.  In addition, the height of the outer peripheral surface (1 la) of the three-way outer wall of the frame-shaped base (11) substantially matches the height of the inner peripheral surface (14a) of the three-way watertight frame of the embedding dish (59). It has an embedding dish opening (2a), There is no watertight frame (1 4) on the other side of the embedding dish, and the lower bottom surface of the cassette frame-shaped substrate (1 1) is formed to be covered with the upper end face (4) of the embedding dish. Has form and function A burr-free embedding dish for a pathological tissue sample. 10. A frame-shaped base (11) having a cassette opening (21) formed on the upper side and a bottom (10) having a porous liquid passage port on the lower side, and a frame-shaped base ( In a cassette for a pathological tissue sample comprising a lid (58) having a porous liquid inlet (58a) removably fitted to the opening (2 1) of 1) and a force,  The outer peripheral surface (11a) of the outer wall of the frame-shaped substrate (11) is fitted to the inner peripheral surface (14a) of the watertight frame of the burrs-free embedding frame (19) of the pathological tissue sample in a watertight manner. An outer peripheral surface (1 la) of the cassette frame-shaped base (11) which has a plate opening (2a) and is a hook portion of an adapter for attaching and detaching the cassette frame-shaped base (11) to the microtome with one touch. ) And the intersection (1 lc) of the outer surface of the porous bottom (1 Ob), the shape of which is to hook the cassette frame-shaped base (11), and the outer peripheral surface of the outer wall of the frame-shaped base (11). 1 la) and the height of the inner wall of the embedding frame (19) almost match,  A bottom plate for an embedding frame (19) or a general-purpose embedding dish (54) is laid on the open bottom (18) of the embedding frame (19), and the closed inner space is used as an embedding dish to obtain a pathological tissue sample. A burr-free embedding frame for a pathological tissue sample, which forms an embedding block. 1 1. A cassette frame base (1 1) with a cassette opening (2 1) formed on the upper side and a cassette bottom (10) with a porous liquid passage port on the lower side. A pathological tissue sample cassette comprising a lid (58) having a porous liquid inlet (58a) removably fitted into the opening (21) of the set frame-shaped substrate (11). , An embedding opening in which the outer peripheral surface (1 la) of the outer wall of the frame-shaped base (11) is fitted in a watertight manner with the inner peripheral surface (14a) of the watertight frame of the pathological sample-free embedding frame (19). (2a), and an outer peripheral surface (1 la) of the cassette frame-shaped base (11), which is a retaining portion of an adapter for detachably attaching the cassette frame-shaped base (11) of the cassette to the microtome with one touch. ) And the intersection (1 lc) of the outer surface of the porous bottom (1 01)) are formed so as to hook the cassette frame-shaped base (1 1), and The height of the inner peripheral surface (14 g) of the watertight frame of the embedding frame (19) is smaller than the height of the outer peripheral surface (1 la) of the outer wall of the frame-shaped base (11); 該包opening of Umawaku (1 ⁹⁾ The thickness of the watertight frame (14) of (2 a) is, the outer peripheral surface (1 1 a of the outer wall of the embedding medium (32) is the frame-like base (1 1) ) And a force to make the contact point (14e) of the watertight frame (14 _g ) of the embedding frame (19) such that it does not accumulate as much as possible, or the opening (2) of the embedding frame (19). The watertight frame (14) of (a) has an embedding dish opening (2a) at the point (14e) of the watertight frame (14e) that is inclined downward from the inside to the outside,  An embedding frame bottom plate (29) or a general-purpose embedding dish (54) is laid on the open bottom (18) of the embedding frame (1 9), and the embedded space is used as an embedding dish to embed a pathological tissue sample embedding block. A burr-free embedding frame, characterized by forming: 1 2. A frame-shaped substrate (11) having a cassette opening (21) formed on the upper side and a bottom surface (10) having a porous liquid passage port on the lower side, and a frame-shaped substrate (1 1). The lid (58) having a porous liquid passage (58a) removably fitted into the opening (21) of the above (21) and a force, such as a pathological tissue sample cassette,  The outer peripheral surface (1 la) of the three-sided outer wall (1 Ob) outside the rectangular porous bottom surface of the frame-shaped substrate (11) is attached to the inner peripheral surface (14) of the burrs-free embedding frame (19) of the pathological sample. a) has an embedding dish opening (2a) that fits tightly with  Intersection between the outer peripheral surface (1 la) of the cassette frame-shaped substrate (1 1), which is a latching portion of the adapter for attaching and detaching the cassette frame-shaped substrate (11) to the microtome with one touch, and the outside of the porous bottom surface (1 Ob). The cassette frame-shaped base (1 1) with the portion (1 lc), and  A three-way watertight frame that fits tightly with the outer peripheral surface (11a) of the three-way outer wall of (11) of the frame-shaped base; There is no watertight frame (14) on one side of the remaining pares-free embedding frame (19), and the lower bottom surface of the cassette frame-shaped base (11) is connected to the upper end surface (4) of the embedding frame. ) And has the form and function of forming a shape to cover An embedding frame bottom plate (19) or a general-purpose embedding dish (54) is laid on the open bottom (18) of the embedding frame (19), and the enclosed inner space is used as an embedding dish to wrap a pathological tissue sample. A burr-free packaging frame characterized by forming an embedded block. 1 3. In the Paris-free embedding frame (1 ⁹ ),  The embedding and storage section (20), which is the space between the pathological tissue sample and the embedding agent filling section surrounded by the inner wall of the embedding frame (19d) and the open bottom (18), should be asymmetrical in the left and right or up and down directions. Molding the inner wall (1 9 d)  Criteria for slicing a thin section of an embedded block of a sample embedded with an embedding medium into left and right or top and bottom asymmetric sections and attaching them to a slide and clarifying the direction in which they extend and the sequence of the samples in the section. The paris-free embedding frame according to claim 10 or 11, wherein the mark (5) is formed into an embedding block. 14. fitting opening of burrs without embedding dish (5 ⁹⁾ and burrs without packaging Umawaku (1 9) (2 a) in a watertight manner, pathologic tissue sample cassette having a cassette frame-like base body (1 1) The cassette frame-shaped substrate (1 1) is attached to and detached from the outer surface (1 la) of the cassette frame-like substrate (1 la) with the microtome in a one-touch manner with respect to the outer surface of the cassette porous bottom (1 Ob). A cassette for a pathological tissue sample, characterized in that the taper has a taper that is slightly obtuse from a right angle that does not hinder installation in a conventional adapter. 1 5. In the embedding dish (59), a burr-free embedding method using a force-set frame base (11), which is fitted in a watertight manner into the opening (2a) of the embedding frame (19). And  A burr-free embedding method, characterized in that a window (22) is formed on the porous bottom surface (10) of the cassette frame-shaped substrate 11. 1 6. The burr-free embedding method using a force-set frame base (11) that fits watertight into the opening (2a) of the embedding dish (59) ゃ embedding frame (19) And A paris-free embedding method, characterized in that a frame-shaped substrate (11) having a porous bottom surface (10) formed in an intermediate portion is used. 1 7. fitted watertight opening ⁽² a) of the burr without embedding dish (5 ⁹⁾ and burrs without packaging Umawaku (1 9), no burr using the cassette frame-like base body (1 1) In the embedding method, the thickness of the frame-shaped substrate (11) is made thinner than the thickness of the conventional cassette frame-shaped substrate, and the embedding frame without burrs and the cassette frame-shaped substrate are integrally fitted in advance. 16. The paris-free embedding method according to claim 14 or 15, wherein the embedding method is performed so that the embedding is performed. 1 8. A window is provided at the porous bottom surface (10) of the cassette frame base (1 1), which fits watertight into the opening (2a) of the embedding dish (59) or embedding frame (19). In the method for embedding burrs without using the cassette for pathological tissue sample formed in (22),  A V-shaped storage part (47) for storing a biopsy sample is formed inside or outside the cassette for pathological tissue samples, and the biopsy samples are arranged there and aligned in a straight line. B). 1 9. Pathological tissue having the cassette frame-shaped substrate (11) that fits tightly into the opening (2a) of the paris-free embedding dish (59) or the burr-free embedding frame (19) In the sample cassette;  For positioning the cassette formed on the inner peripheral surface of the burr-free embedding dish (59) or the burr-free embedding frame (19), on the opposing outer peripheral surface (1 la) of the cassette frame-shaped substrate (11). Stopper A cassette for a pathological tissue sample characterized by forming a recess for positioning a cassette (ie, a recess), which is detachably fitted to the projection (61). 20. In the above described burr-free embedding dish (59) or the burr-free embedding frame (19), which is fitted in a watertight manner with the cassette frame-shaped substrate (11), A cassette positioning stopper formed on the outer peripheral surface (1 la) of the cassette frame base (11) on the inner peripheral surface of the burrs-free embedding dish (59) or the burrs-free embedding frame (19). for one indentation (l ie), unlatched freely fitted to burrs without packaging and forming the cassette positioning Sutotsu par projection (61) Umasara ⁽⁵ 9), or no the Paris follicles Buried frame.