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WO2004018662A3 - Cellulases and hemicellulases and uses thereof - Google Patents

Cellulases and hemicellulases and uses thereof Download PDF

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Publication number
WO2004018662A3
WO2004018662A3 PCT/EP2003/009147 EP0309147W WO2004018662A3 WO 2004018662 A3 WO2004018662 A3 WO 2004018662A3 EP 0309147 W EP0309147 W EP 0309147W WO 2004018662 A3 WO2004018662 A3 WO 2004018662A3
Authority
WO
WIPO (PCT)
Prior art keywords
hemi
cellulase
sequence
novel
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2003/009147
Other languages
French (fr)
Other versions
WO2004018662A2 (en
Inventor
Ulrike Folkers
Andreas Fritz
Beatrix Gerhard
Sabine Klugbauer
Fabio Spreafico
Christian Wagner
De Lex Boer
Roelf Bernhard Meima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Priority to AU2003251710A priority Critical patent/AU2003251710A1/en
Publication of WO2004018662A2 publication Critical patent/WO2004018662A2/en
Publication of WO2004018662A3 publication Critical patent/WO2004018662A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01113—Mannosyl-oligosaccharide 1,2-alpha-mannosidase (3.2.1.113), i.e. alpha-1,2-mannosidase
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01055—Alpha-N-arabinofuranosidase (3.2.1.55)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01058—Glucan 1,3-beta-glucosidase (3.2.1.58)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel (hemi)cellulase from Aspergillus niger The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel (hemi)cellulase as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a (hemi)cellulase according to the invention is genetically modified to enhance its activity and/or level of expression.
PCT/EP2003/009147 2002-08-19 2003-08-15 Cellulases and hemicellulases and uses thereof Ceased WO2004018662A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003251710A AU2003251710A1 (en) 2002-08-19 2003-08-15 Cellulases and hemicellulases and uses thereof

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
EP02102161.3 2002-08-19
EP02102152 2002-08-19
EP02102161 2002-08-19
EP02102160 2002-08-19
EP02102152.2 2002-08-19
EP02102160.5 2002-08-19
EP02102245.4 2002-08-30
EP02102245 2002-08-30
EP02102298.3 2002-09-03
EP02102298 2002-09-03
EP02102279 2002-09-03
EP02102279.3 2002-09-03

Publications (2)

Publication Number Publication Date
WO2004018662A2 WO2004018662A2 (en) 2004-03-04
WO2004018662A3 true WO2004018662A3 (en) 2004-04-08

Family

ID=31950906

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/009147 Ceased WO2004018662A2 (en) 2002-08-19 2003-08-15 Cellulases and hemicellulases and uses thereof

Country Status (2)

Country Link
AU (1) AU2003251710A1 (en)
WO (1) WO2004018662A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101276755B1 (en) 2005-05-04 2013-06-20 아디쎄오 프랑스 에스에이에스 abfB-2 gene of Penicillium funiculosum
RU2850538C1 (en) * 2019-08-30 2025-11-12 Аб Энзаймс Гбмх Use of cellulase gh12 in baking products made from rye flour

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2885366B1 (en) * 2005-05-04 2007-06-15 Adisseo France Sas Soc Par Act ABFB-1 GENE OF PENICILLIUM FUNICULOSUM
EP2073642B1 (en) 2006-09-29 2015-11-11 Novozymes A/S Xylanases for animal feed
US8088549B2 (en) 2007-12-19 2012-01-03 Eastman Kodak Company Radiation-sensitive elements with developability-enhancing compounds
US9187742B2 (en) 2010-08-30 2015-11-17 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2012030799A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2012030844A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
US8624082B2 (en) 2010-08-30 2014-01-07 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
WO2012030845A2 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
NL2008057C2 (en) 2011-12-29 2013-07-03 Konink Zeelandia Groep B V Improved methods, products and uses relating to the preparation of dough.
BR112022002555A2 (en) 2019-08-30 2022-06-14 Ab Enzymes Gmbh Use of gh12 cellulases in the preparation of bakery products comprising rye flour
CN110613005A (en) * 2019-09-26 2019-12-27 安徽农业大学 Agaricus bisporus bread and preparation method thereof
EP4052581A1 (en) 2021-03-03 2022-09-07 AB Enzymes GmbH Use of gh12 cellulases in spelt, oat, barley, and / or millet baking
EP4285728A1 (en) * 2022-05-31 2023-12-06 Kerry Group Services International Limited Dough composition comprising non-starch polysaccharide degrading enzymes

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999006574A1 (en) * 1997-07-31 1999-02-11 Dsm N.V. Cellulose degrading enzymes of aspergillus
EP0913092A2 (en) * 1997-10-31 1999-05-06 Amano Pharmaceutical Co., Ltd. Dough composition and preparation thereof
WO2001025406A1 (en) * 1999-10-01 2001-04-12 University Of Victoria Innovation & Development Corporation Mannosidases and methods for using same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999006574A1 (en) * 1997-07-31 1999-02-11 Dsm N.V. Cellulose degrading enzymes of aspergillus
EP0913092A2 (en) * 1997-10-31 1999-05-06 Amano Pharmaceutical Co., Ltd. Dough composition and preparation thereof
WO2001025406A1 (en) * 1999-10-01 2001-04-12 University Of Victoria Innovation & Development Corporation Mannosidases and methods for using same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
INOUE T ET AL: "MOLECULAR CLONING AND NUCLEOTIDE SEQUENCE OF THE 1,2-ALPHA-D-MANNOSIDASE GENE, MSDS, FROM ASPERGILLUS SAITOI AND EXPRESSION OF THE GENE IN YEAST CELLS", BIOCHIMICA ET BIOPHYSICA ACTA. PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, ELSEVIER, AMSTERDAM,, NL, vol. 1253, 1995, pages 141 - 145, XP002946046, ISSN: 0167-4838 *
SANDER I ET AL: "ALLERGY TO ASPERGILLUS-DERIVED ENZYMES IN THE BAKING INDUSTRY: IDENTIFICATION OF BETA-XYLOSIDASE FROM ASPERGILLUS NIGER AS A NEW ALLERGEN (ASP N 14)", JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, MOSBY - YEARLY BOOK, INC, US, vol. 102, no. 2, August 1998 (1998-08-01), pages 256 - 264, XP009021145, ISSN: 0091-6749 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101276755B1 (en) 2005-05-04 2013-06-20 아디쎄오 프랑스 에스에이에스 abfB-2 gene of Penicillium funiculosum
RU2850538C1 (en) * 2019-08-30 2025-11-12 Аб Энзаймс Гбмх Use of cellulase gh12 in baking products made from rye flour

Also Published As

Publication number Publication date
WO2004018662A2 (en) 2004-03-04
AU2003251710A8 (en) 2004-03-11
AU2003251710A1 (en) 2004-03-11

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