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WO2004011906A2 - Systeme de diagnostic pour differencier l'expectoration de la salive - Google Patents

Systeme de diagnostic pour differencier l'expectoration de la salive Download PDF

Info

Publication number
WO2004011906A2
WO2004011906A2 PCT/US2003/023514 US0323514W WO2004011906A2 WO 2004011906 A2 WO2004011906 A2 WO 2004011906A2 US 0323514 W US0323514 W US 0323514W WO 2004011906 A2 WO2004011906 A2 WO 2004011906A2
Authority
WO
WIPO (PCT)
Prior art keywords
sample
sputum
sputa
diagnostic system
patient
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/023514
Other languages
English (en)
Other versions
WO2004011906A3 (fr
Inventor
Parker Small
Darla J. Gaskins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Florida
Original Assignee
University of Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Florida filed Critical University of Florida
Priority to AU2003259266A priority Critical patent/AU2003259266A1/en
Publication of WO2004011906A2 publication Critical patent/WO2004011906A2/fr
Publication of WO2004011906A3 publication Critical patent/WO2004011906A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Definitions

  • a physician obtains a sample of sputa from a patient complaining of discomfort and showing signs of a pulmonary disease or condition.
  • Sputum is matter is matter produced in respiratory tract. Sputum comes in a variety of colors and forms.
  • Fresh sputum originating from the lungs should contain pathogens if the patient is suffering from a pulmonary infection or condition. Sputum generally accumulates in the lungs as a result from the progression of such an infection. The following list identifies commonly known variations of sputum.
  • test strips preferably utilizes test strips (Product Code 5122) by SerimTM or the MULTISTIX ® reagent strips by Bayer Corporation ® .
  • Leukocyte esterase or protease activity in pulmonary diseases or conditions increases when leukocyte counts increase in response to pulmonary infections typically caused by bacterial pathogens.
  • SERIMTM test strips are designed to give a semi-quantitative indication of the level of leukocytes present in a peritoneal dialysate effluent, thereby providing a presumptive indication of peritonitis.
  • the diagnostic system of the present invention can utilize the indicative qualities of these test strips to diagnose a different disease or infection through a different bodily fluid and different method of collecting the bodily fluid thereof.
  • test strips or sticks used in the present invention include, but are not limited to, by the reaction of a phenyl pyrrole ester (or an indoxyl ester).
  • esterase in granulocytic leukocytes catalyzes the hydrolysis of a modified pyrrole amino acid ester to yield 3-hydroxy-5-phenyl pyrrole.
  • the released 3-hydroxy-5-phenyl pyrrole then reacts with diazonium salt to produce a purple color (shown in the chemical reaction below).
  • the patient provides to the physician or medical technician at least one sample of sputum originating from the lungs via the nasal cavity or the oral cavity which is deposited into a clean receptacle.
  • 3. Completely immerse the indicator pad of the test strip into the sample, remove immediately and start a timer (time period of immersion depends on the test strip assay utilized with the present invention). 4. Place the test strip (indicator pad facing up) on a flat, clean surface.
  • test strip assays specific in detecting the presence of leukocyte esterase activity are also designed to give a semi-quantitative indication of the level of leukocytes present based on a specific bodily fluid such as urine, saliva, and blood based on in the color of the reacted indicator pad.
  • a specific bodily fluid such as urine, saliva, and blood based on in the color of the reacted indicator pad.
  • current test strip assays are not designed for sputa samples, the present method can incorporate the test strips to the diagnostic system of the present invention.
  • test strip or stick assay to differentiate a sputa sample from a saliva sample allows medical personnel to accurately and rapidly send sputa samples containing sputum for analysis of pathogens.
  • the test strip assays produce a color change which can be seen by the naked eye to indicate that luekocyte esterase or protease activity is present in the sample. This activity indicates that the sputa sample obtained from the patient contains sputum. Sputum matter is the result of an infection which contains bacterial pathogens that are associated with many varieties of pulmonary diseases or conditions. Without the use of this test strip method of the present invention, one out of every three sputa samples submitted to clinical laboratories for testing are unsatisfactory. This can cause delay in treatment and unnecessary repetitive testing.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un nouveau système de diagnostic permettant de différencier rapidement et de manière non invasive et peu coûteuse l'expectoration de la salive. Les procédés selon l'invention font appel à une bandelette d'essai, ou utilisent une analyse de bâtonnet ou d'autres analyses analogues, pour détecter la présence d'estérases leucocytaires qui se rapportent à la présence d'expectoration dans un échantillon du patient concerné.
PCT/US2003/023514 2002-07-30 2003-07-29 Systeme de diagnostic pour differencier l'expectoration de la salive Ceased WO2004011906A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003259266A AU2003259266A1 (en) 2002-07-30 2003-07-29 Diagnostic system for differentiating sputum from saliva

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/209,396 2002-07-30
US10/209,396 US20040029205A1 (en) 2002-07-30 2002-07-30 Diagnostic system for differentiating sputum from saliva

Publications (2)

Publication Number Publication Date
WO2004011906A2 true WO2004011906A2 (fr) 2004-02-05
WO2004011906A3 WO2004011906A3 (fr) 2004-07-08

Family

ID=31187037

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/023514 Ceased WO2004011906A2 (fr) 2002-07-30 2003-07-29 Systeme de diagnostic pour differencier l'expectoration de la salive

Country Status (3)

Country Link
US (1) US20040029205A1 (fr)
AU (1) AU2003259266A1 (fr)
WO (1) WO2004011906A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007027234A3 (fr) * 2005-08-31 2007-06-07 Kimberly Clark Co Technique de detection d'enzymes
US8003399B2 (en) 2005-08-31 2011-08-23 Kimberly-Clark Worldwide, Inc. Nitrite detection technique

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7906276B2 (en) 2004-06-30 2011-03-15 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
US7521226B2 (en) 2004-06-30 2009-04-21 Kimberly-Clark Worldwide, Inc. One-step enzymatic and amine detection technique
US7402423B2 (en) * 2004-08-13 2008-07-22 Biomed Solutions, Llc Apparatus for the detection of pepsin
US20060127459A1 (en) * 2004-12-15 2006-06-15 Lei Huang Urogenital infection inhibition
US8758989B2 (en) * 2006-04-06 2014-06-24 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
US7897360B2 (en) 2006-12-15 2011-03-01 Kimberly-Clark Worldwide, Inc. Enzyme detection techniques
WO2016054079A1 (fr) 2014-09-29 2016-04-07 Zyomed Corp. Systèmes et procédés pour la détection et la mesure du glucose sanguin du sang et d'autres analytes à l'aide du calcul de collision
US9554738B1 (en) 2016-03-30 2017-01-31 Zyomed Corp. Spectroscopic tomography systems and methods for noninvasive detection and measurement of analytes using collision computing
CN112326641B (zh) * 2020-07-29 2024-07-05 浙江今复康生物科技有限公司 一种从咳出样品中快速鉴别痰液成分的方法及试剂盒

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5464739A (en) * 1994-08-22 1995-11-07 Bayer Corporation Composition method for determining the presence of leukocyte cells, esterase or protease in a test sample
US5910421A (en) * 1995-12-21 1999-06-08 University Of Florida Rapid diagnostic method for distinguishing allergies and infections

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007027234A3 (fr) * 2005-08-31 2007-06-07 Kimberly Clark Co Technique de detection d'enzymes
US7504235B2 (en) 2005-08-31 2009-03-17 Kimberly-Clark Worldwide, Inc. Enzyme detection technique
US8003399B2 (en) 2005-08-31 2011-08-23 Kimberly-Clark Worldwide, Inc. Nitrite detection technique

Also Published As

Publication number Publication date
AU2003259266A1 (en) 2004-02-16
US20040029205A1 (en) 2004-02-12
WO2004011906A3 (fr) 2004-07-08
AU2003259266A8 (en) 2004-02-16

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