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WO2004009496A2 - Unite portable/mobile de traitement d'eaux usees - Google Patents

Unite portable/mobile de traitement d'eaux usees Download PDF

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Publication number
WO2004009496A2
WO2004009496A2 PCT/US2003/022524 US0322524W WO2004009496A2 WO 2004009496 A2 WO2004009496 A2 WO 2004009496A2 US 0322524 W US0322524 W US 0322524W WO 2004009496 A2 WO2004009496 A2 WO 2004009496A2
Authority
WO
WIPO (PCT)
Prior art keywords
nitrifier
bioreactor
water
unit
nutrient
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/022524
Other languages
English (en)
Other versions
WO2004009496A3 (fr
Inventor
Bob Beine
John Friel
Mark Pesonen
Jon Shaffer
Jack Fernandes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aqua-Novus Corp
Aqua-Nova LLC
Original Assignee
Aqua-Novus Corp
Aqua-Nova LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aqua-Novus Corp, Aqua-Nova LLC filed Critical Aqua-Novus Corp
Priority to AU2003252042A priority Critical patent/AU2003252042A1/en
Publication of WO2004009496A2 publication Critical patent/WO2004009496A2/fr
Publication of WO2004009496A3 publication Critical patent/WO2004009496A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/30—Aerobic and anaerobic processes
    • C02F3/302—Nitrification and denitrification treatment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/12—Activated sludge processes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
    • C02F2203/008—Mobile apparatus and plants, e.g. mounted on a vehicle
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/02—Temperature
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/06—Nutrients for stimulating the growth of microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Definitions

  • a portable/mobile biological treatment and water recycling apparatus comprising a shed, cabinet, truck, pickup, trailer or other wheeled container having at least one biological wastewater treatment unit, aeration unit, biological nitrification unit, and circulating pump for circulating the wastewater to be treated.
  • the biological wastewater treatment unit introduces pre-conditioned living organisms to the environment to be treated in order to biodegrade the waste.
  • the nitrification unit introduces pre-conditioned living nitrifying organisms for the biological breakdown of nitrogen-based wastes, such as ammonia, nitrates, nitrites and other contaminants contained in waste water streams, ground water, soil, lagoons, etc.
  • the circulating pump circulates the wastewater in the environment to be treated aerating the wastewater and floating digestible solids.
  • the aeration unit pumps dissolved oxygen into the wastewater environment so that aerobic respiration can take place.
  • Neighbors find odors emanating from lagoons, confinement houses, and fields onto which wastes are sprayed to be a nuisance. In fact, as a result of odor problems associated with anaerobic lagoons, certain states have legally mandated buffer zones or designated land areas between lagoon sites and populated areas.
  • the present invention relates to a portable/mobile biological treatment and water- recycling apparatus comprising a shed, cabinet, truck, pickup, trailer or other wheeled container having at least one biological wastewater treatment unit, aeration unit (preferably an air injection unit), biological nitrification unit, and circulating pump for circulating the wastewater to be treated. More specifically, the present invention relates to a portable/mobile biological wastewater treatment unit that incubates and dispenses large amounts of nitrifying microorganisms and organic digesting microorganisms in log-growth phase.
  • the nitrifying microorganisms biologically digest the nitrogenous waste during a process called nitrification that takes place in the aerobic environment. This sets the stage for the final removal of nitrogen from the environment to be treated via an anaerobic de- nitrification process.
  • the ammonia content in the environment to be treated is converted to nitrate, utilizing the organic carbon content of the wastewater.
  • the nitrate is converted to gaseous nitrogen, also requiring the use of organic carbon.
  • the environment to be treated after nitrification does not contain sufficient organic carbon for effective de-nitrification. Accordingly it is frequently necessary to add supplemental organic carbon to the de-nitrification zone, commonly in the form of methanol.
  • the organic digesting microorganisms aerobically metabolize organic carbon waste in the environment to be treated into carbon dioxide, water and inorganic side products. Even though the biologically mediated processes of nitrification and de-nitrification, and conversion of ammonia to free nitrogen are well known, improvements in conventional methods of treating wastewater containing high levels of ammonia, organic material and other contaminants are still needed. The research and development to upgrade the performance of biological treatment systems to handle high ammonia containing organic liquids as well as organic material has been basically considered unsuccessful to date.
  • the activated sludge system is the operation of handling large amounts of sludge.
  • This system treats the sludge by circulating the nitrogen containing waste into a tank, treating the tank with nitrifying microorganisms and then circulating the treated wastes back into the environment being treated.
  • This system does not simultaneously treat the waste with organic waste digesting microorganisms. Therefore the organic material is unaffected.
  • circulating sludge from a food processing plants and/or lagoons used to collect animal waste in an animal harvesting operating is extremely energy inefficient and costly. And treating the waste for high concentrations of nitrogen-based compounds does not completely solve the problem of waste build-up.
  • an animal waste containing 5 acre lagoon holds over 10 million gallons of waste and it would take an enormous amount of energy to direct the waste into holding tanks so that they can be treated with nitrifying microorganisms and released. This process is simply cost prohibited.
  • wastes under normal conditions, are gradually broken down or biodegraded by indigenous microorganisms in the environment.
  • biodegradation reactions are often hindered by environmental fluctuations such as changes in temperature, pH, salinity, water and air supply, etc.
  • wastes such as fat and grease are biodegraded by microorganisms to fatty acids and glycerol.
  • the fatty acids are further metabolized with the end product being carbon dioxide and inert by-products.
  • Glycerol is also metabolized as an efficient energy source.
  • nitrifying microorganisms are not normally present in high enough levels to effectively breakdown nitrogenous waste.
  • waste lagoons are anaerobic and nitrifying microorganisms only exist in aerobic environments, the majority of waste lagoons do not naturally contain nitrifying microorganisms.
  • a portable/mobile all-in-one on-site apparatus that produces large amounts of activated nitrifying microorganisms in log growth phase as well as large amounts of activated organic microorganisms in log growth phase overcomes the shortcomings of existing systems.
  • the present invention provides a portable/mobile wastewater bio-augmentation system that adjusts the environment to be treated to a condition that is more conducive for bio-degradation of waste by introducing activated microorganisms designed to digest organic waste as well as nitrogen-based waste.
  • Activated microorganisms are microorganisms that are in the exponential phase of growth. These microorganisms are more efficient in the biodegradation of waste than microorganisms that are not in the exponential phase of growth.
  • the present invention provides methods and apparatuses for the continuous culturation of evolving bacterial consortia and by-products for direct utilization in bioremedial and bioaugmentation applications, such that the digestion and mobilization of organic waste and nitrogen-based waste in plants, lagoons, and treatment systems or natural contaminated sites are treated.
  • the portable/mobile biological treatment and water recycling apparatuses include a portable/mobile transport device such as a shed, cabinet, truck, pickup, trailer or other wheeled container, a bioreactor nitrifier for activating and growing microorganisms that digest organic waste, a nutrient reservoir source for feeding the microorganisms in the bioreactor, a bioreactor nitrifier for activating and growing nitrifying microorganisms and facultative anaerobic de-nitrifying microorganisms, a nutrient reservoir source for feeding the nitrifying microorganisms in the bioreactor, an aeration unit (preferably an air injection unit), a circulating pump for circulating the wastewater to be treated and a controller for managing and monitoring the continuous delivery of the biology from bioreactors as well as the operation of the circulating pumps.
  • a portable/mobile transport device such as a shed, cabinet, truck, pickup, trailer or other wheeled container
  • a portable/mobile apparatus for delivering microorganisms to an environment to be treated comprising a structure having an interior space, a bioreactor vessel having a bioreactor output port positioned within the structure, a nutrient container comprising a mixture of inorganic and organic nutrients positioned within the structure, a nutrient pumping means for pumping inorganic and organic nutrients from the nutrient container to the bioreactor, a water pumping means for pumping water into the bioreactor, the water pumping means is in fluid communication with the bioreactor and a water source wherein the water pumped into the bioreactor displaces fluid out of the bioreactor output port, a recirculation pump positioned within the structure in fluid communication with the environment to be treated,
  • the portable/mobile wastewater bioaugmentation system is equipped with a continuous monitoring system that monitors functioning of the components of the system and electronically notifies, via e-mail, pager alerts and/or cell phone calls, whether a component of the system has malfunctioned or failed.
  • the monitoring system can also be used to send data from sensors positioned either in the bioreactor, nitrifier or in the environment being treated to a home base. This information can be used to access the condition/state of the bioreactor or nitrifier, access the condition of the environment to be treated and/or simply to collect real time data from the environment to be treated.
  • the portable/mobile wastewater bioaugmentation system is connected to a distribution/delivery system that delivers the growing microorganisms that digest organic waste and/or nitrifying microorganisms and/or facultative anaerobic de-nitrifying heterotrophs to the environment to be treated in an effective, comprehensive way.
  • the delivery system comprises a pump-manifold system that is situated around, within and/or above the site being treated so as to deliver the microorganisms and oxygen most effectively.
  • the manifold system may consist of polyvinylchloride (PVC) piping, flexible hose (or equivalent material), oxygen generating restriction nozzles (eductor nozzles), gate valves and spray nozzles.
  • PVC polyvinylchloride
  • the design of the manifold is important for the stability and reproductive capabilities of the microorganisms, as well as the oxygen transfer. It is important that the manifold be designed so that the oxygen and microorganisms are delivered above and below the surface of the treatment site to assure proper flow rates so as to achieve hydraulic shearing and oxygen saturation of the recycled waste water.
  • An apparatus comprises six main subsystems: a portable/mobile container; a controller unit; a bio-augmentation system for growing organic digesting microorganisms; a bio-augmentation system for growing nitrifying microorganisms; potable water reservoir and regulator; and a recirculation pump.
  • a portable/mobile container for storing organic digesting microorganisms
  • a bio-augmentation system for growing nitrifying microorganisms
  • potable water reservoir and regulator a recirculation pump.
  • the portable/mobile container used to house and transport the wastewater bio- augmentation system of the present invention may be selected from the group consisting essentially of shed, cabinet, trailer, pickup truck, boat, barge, truck, or a wheeled container.
  • the portable/mobile container chosen will depend on the environment and conditions of the environment where the unit will be deployed. Regardless of the portable/mobile container chosen, the container will be modified so as to house the components of the wastewater treatment system described below.
  • the portable/mobile container may be equipped with a heating system and/or a cooling system controlled by a thermostat and or the controller. The heating/cooling system will maintain the internal temperature of the portable/mobile unit within a pre-determined range.
  • the portable/mobile container shall be equipped with a controller unit that houses and protects electronic components and isolates electrical components, along with fuses and electrical boards, and connections for safe operation of the system and to comply with applicable standards.
  • the controller is in communication with the other main subsystems of the portable/mobile wastewater treatment system. More specifically, the controller will regulate the function of the pumping systems of water, nutrients, and dosing in the bio- augmentation system for growing organic digesting microorganisms as well as the bio- augmentation system for growing nitrifying and de-nitrifying microorganisms.
  • the controller also controls the recirculation pump of the system.
  • the types and numbers of pumps are not critical.
  • a pump may be positioned to operate each input into the organic digesting bioreactor and/or the nitrifier, e.g. from water reservoir, nutrient container and atmosphere (to provide air to the bioreactor, for instance, to enable aeration).
  • the pumps may be single or multi-chambered pumps or other appropriate pumping mechanism known in the art.
  • the bio-augmentation system for growing organic digesting microorganisms comprises at least one bioreactor that provides an environment conducive for the culturation of microbes, and includes at least one container.
  • the bioreactor may include one or more of the following: a heater (such as a thermostatically controlled heater), a temperature control, an aeration means, organic and inorganic nutrients, check valves for isolation of the bioreactor, and pipes and/or tubing to provide for aeration, water, dosing of the microorganisms to a dosing tank and for delivery of the nutrient formulation to the bioreactor.
  • the dosing cycles for potable water and nutrient delivery to bioreactor are set to the same number of cycles per day, generally 4, 8 to 16, preferably 6 to 12. These cycles are controlled by the turning on and off of the nutrient and water pumps connected to the bioreactor and are controlled by the controller or a timer.
  • the controller can be programmed to turn the nutrient pumps on and off at different times of the day. Therefore, in a 2-timer embodiment, nutrient delivery to the bioreactor should lag water delivery by 15 to 60 minutes, depending on the spacing of cycles.
  • the ratio of amount of potable water (by volume) to nutrient should be between 50 to 200, preferably 75 to 125.
  • the requisite ratio may be achieved by calibrating the respective transfer pumps accordingly.
  • the total daily volume of effluent produced is approximately equal to the total daily volume of potable water and nutrients delivered to the bioreactor.
  • the potable water delivered to the bioreactor should be set from about 1 to about 12 times the total volume of bioreactor, preferably from about 2 to about 6 times the volume.
  • the temperature of the bioreactor is set on a heater and should be from about 60 degrees F to about 120 degrees F, preferably from about 80 degrees F to about 100 degrees F.
  • Dosing of activated microorganisms from the bioreactor into the dosing tank of the portable/mobile system occurs continuously and steps up about 4 to about 16 times a day, preferably about 6 to about 12 times a day, most preferably about 4 to about 8 times a day.
  • the continuous bioreactor system doses continuously and is stepped up when the composition is displaced from the bioreactor upon addition of water into the bioreactor.
  • Once dosed into the dosing tank the activated log-phased microorganisms enter the environment to be treated and begin to digest organic material immediately. The process may be initiated by starting water and nutrient flow to the bioreactor used to produce activated log-phase microorganisms that digest non-nitrogen containing organic contaminants.
  • the bioreactor compartment is sterilized by cleaning it with, or otherwise applying, a suitable disinfectant agent to the surface.
  • a suitable disinfectant agent for instance, a 70% ethanol solution, or some similar alcohol based surface active disinfectant may be used.
  • a suspended bacterial product that is designed to produce microorganisms that digest non-nitrogen organics waste, either in desiccated or in liquid spore-like form, is added to the bioreactor compartment.
  • the bacterial product is enclosed in a 1/16 inch steel mesh ball, and contains from about 1 to about 15 grams of bacterial composition.
  • the bioreactor is then sealed and all connections are capped off until after installation.
  • the microorganisms employed in the starter material may vary, but all of which digest non-nitrogen containing organic waste material.
  • the starter material contains at least one microorganism selected from the group consisting essentially of Bacillus licheniformis. Bacillus subtilis, Pseudomonas fluorescens E, Pseudomonas putida, Enterobacter cloacae, and Bacillus thuringienis.
  • a preferred bacterial product that may be employed is Bi-Chem® SM 700 from Sybron, Inc., of Salem, Va.
  • the starter material generally has a concentration of cells of at least ⁇ 1 x 10 8 per fluid ml as well as the essential inorganic and organic nutrients to maintain the cell culture in the exponential phase of growth.
  • the content and concentration of the inorganic and organic nutrients in the food will vary with the type of microorganism used in the apparatus.
  • a composition containing organic and inorganic nutrients may be in either a liquid or solid (e.g. powdered) matter state. If a liquid, the formula may have a pre-determined concentration, the preferred concentration is on the order of 100 times.
  • An embodiment of a composition containing organic and inorganic nutrients that is used as part of a starter material generally includes a metal-oleate, preferably K-oleate, and one or more of magnesium sulfate, calcium chloride, potassium phosphate, sodium phosphate, sodium EDTA, sodium hydroxide, ferric NH citrate, potassium bicarbonate, sodium chloride, dextrose, citrate, yeast extract, whey extract, ketrol, ammonium nitrate, ammonium chloride, glycerin, Tween 20, Tween 80, corn oil, Simethlycone, and trace elements that include but are not limited to copper sulfate, cobalt(fl) chloride, Sodium EDTA, Molybolic acid, MnCl 2 -7H 2 0, and zinc sulfate.
  • a metal-oleate preferably K-oleate
  • magnesium sulfate calcium chloride
  • potassium phosphate sodium phosphate
  • sodium EDTA sodium
  • the composition containing organic and inorganic nutrients includes about 50 to about 60 weight % of water, about 20 to about 30 weight % K-oleate, about 2 to about 3 weight % glycerin, about 3 to about 10 weight % of vegetable oil and less than about 1 weight % of compounds selected from the group consisting essentially of MgSO 4 , CaCl 2 , NaHPO 4 - 7H 2 0, K 2 HP0 4 , NaCl, Dextrose, Citrate, Yeast Extract, Whey Extract, Trace elements, Sodium EDTA, Keltrol, Ferric NEcitrate, NaOH, NH 4 NO 3 , NH4C1, Tween 20, Tween 80, and Simetblycone.
  • MgSO 4 CaCl 2 , NaHPO 4 - 7H 2 0, K 2 HP0 4 , NaCl, Dextrose, Citrate, Yeast Extract, Whey Extract, Trace elements, Sodium EDTA, Keltrol, Ferric NEcitrate,
  • the vegetable oil is a mixture of about 4 to about 5 weight % of corn oil and about 5 to about weight 6% canola oil peanut oil.
  • the composition containing organic and inorganic nutrients can be prepared by mixing metal-oleate, glycerin, Tween 20, Tween 80, water, and Keltrol in a mixing kettle. MgSO 4 , CaCl 2 , Sodium EDTA is added to 1 gallon of water and the pH is brought to about 8 to about 10, preferably about 9 using about ION NaOH. This mixture is then added to the mixing kettle and is mixed for about 2 minutes. To about 5 gallons of water the Na 2 HPO 4 - H 2 0 and K 2 HP0 4 is added. The pH is brought to about 8 to about 10, preferably about 9 using about ION NaOH. This mixture is added to the mixing kettle after 2 minutes of mixing.
  • Sodium EDTA and ferric NHcitrate is dissolved in about 200ml of hot water and then added to the composition.
  • the 8-gallon mixture brings the pH of the total composition to a pH of from about 9 to about 10, preferably about 9.
  • the mixture is then added to the mixing kettle.
  • corn oil and canola oil are added to the mixing kettle, and NH 4 NO 3 and ISfflUCl are sprinkled into the mixing kettle.
  • the combined mixture is mixed thoroughly and filled into a dispensing container immediately.
  • An anti- foaming agent may then be added.
  • the pH of the final mixture should be from about 9 to about 10, preferably from about 9.3 to about 9.6.
  • at least one microorganism is added.
  • the microorganism may be selected from the group of microorganisms (and may consisting essentially of (or consist of) Bacillus licheniformis, Bacillus subtilis, Pseudomonas fluorescens E, Pseudomonas putida, Enterobacter cloacae, and Bacillus thuringienis. and may be added prior to inoculation of the bioreactor. It is within the scope of the invention to substitute microorganism not listed that are capable of digesting waste.
  • the portable/mobile wastewater treatment unit may also include a nitrification and de-nitrification system.
  • the nitrification/de-mtrification system comprises a bioreactor that provides an environment conducive for the cultivation of nitrifying microorganisms as well as assorted facultative anaerobic heterotrophs.
  • the heterotrophs are capable of de- nitrification and stabilize and buffer the system.
  • the facultative anaerobic heterotrophs help with de-nitrification when the oxygen concentration is reduce or shut-off and therefore aide in keeping the pH of the system within a predetermined range.
  • the hetertrophs help with the production of bio-films that are important in properly seeding the environment being treated.
  • the bioreactor may include one or more of the following: a heater (such as a thermostatically controlled heater), a temperature control, an aeration means, organic and inorganic nutrients, a recirculation pump attached to inductor mixing nozzles, a pH buffer port, a pH buffer pump, a booster inoculation port, a booster inoculation pump, a pH probe, an inspection sampling port, an inoculation port, an inoculation pump, potable water input, an attached growth substrate for increased surface area, check valves for isolation of the bioreactor, and pipes and/or tubing to provide for aeration, nutrients, booster inoculations and dosing.
  • a heater such as a thermostatically controlled heater
  • a temperature control such as a thermostatically controlled heater
  • an aeration means such as a thermostatically controlled heater
  • organic and inorganic nutrients such as a recirculation pump attached to inductor mixing nozzles
  • a pH buffer port such as a thermostatically controlled heater
  • the dosing cycles for potable water and nutrient delivery to bioreactor are either set to the same number of cycles per day, generally 4, 8 to 6, preferably 6 to 12.
  • the same number of cycles may not occur each day.
  • the first container comprising Media A that generally includes (NH 4 ) 2 S0 4 ; K 2 HPO 4 ; Fe;MgSO 4 ;CaCl;EDTA; and trace elements that include but are not limited to CuS04, Co(II)Cl, Molybolic acid, MnCl 2 -7H 2 0, and ZnSO4 is dosed to the bioreactor every 2-4 hours.
  • the second container comprising Media B that generally includes KHC0 3 ; a sugar source (e.g. dextrose); and an amino acid source (e.g. peptone) is also dosed every 2-4 hours and is slightly off-phase with the cycle of the first container.
  • both Media A and Media B are in the same container and are dosed every 2-4 hours.
  • the size of the bioreactor can range from several liters to several hundred gallons. Assuming the bioreactor is 25 gallons then 50 ml of Media A and 50 ml of Media B is added to the bioreactor every 2-4 hours. As the bioreactor increases in size the amount of Media A, and Media B increases proportionally to the increased size of the bioreactor nitrifier.
  • the ratio of amount of potable water (by volume) to Media A, Media B the Media should be between about 25 to about 50, preferably 40.
  • the ratio of amount of potable water (by volume) to Media C should be between 75-150, preferably 140.
  • the requisite ratio may be achieved by calibrating the respective transfer pumps accordingly.
  • the total daily volume of effluent produced is equal to the total daily volume of potable water delivered to the bioreactor.
  • Booster inoculations may be used to assure proper seeding of the nitrification bioreactor. These booster inoculations may be continuing for the first month of operation or may continued until determined unnecessary.
  • nitrifying consortium may be pumped via the inoculating booster pump through the inoculating booster port into the bioreactor up to 10 times a day. If a less concentrated nitrifying consortium is used a proportional amount of nitrifying consortium should be used.
  • the potable water delivered to the bioreactor each day should be set from about 1 to about 1/12 of the total volume of the bioreactor, preferably from about 1/2 to about 1/9 of the total volume of the bioreactor, more preferably about 1/7 of the total volume of the bioreactor.
  • the temperature of the bioreactor is set on a heater and should be from about 60 degrees F to about 120 degrees F, preferably from about 80 degrees F to about 100 degrees F.
  • the process may be initiated by starting water and nutrient flow to the bioreactor. For instance, by cycling the nutrient and water pumps enough times to fill the bioreactor. This, along with the heat and aeration, will start the germination process. After initiation, there will be copious bubbling of the composition in the bioreactor, now inoculated with microbes. The resulting aeration aids the overall growth and colonization of the nitrification microorganisms. In time, the microorganism substrate utilization rates (of nitrogen, and also protein and carbohydrates) approach that of delivery rates. The organization of the microorganism consortium will in general continually tend to improve overall over time as the microorganisms co-adapt to maximize target type nitrogen utilization.
  • the process of the invention involves pumping of nutrients from the nutrient container by a nutrient pumping means into the bioreactor nitrifier via tubes or pipes. Water is pumped to the bioreactor nitrifier, thus displacing microbes, organic and inorganic nutrients and bacterial products into a dosing tank that will be dosed toward the environment to be treated. This discharge may be timed and/or suitably regulated. The cycle then repeats according to intervals set on the timer(s).
  • the bioreactor compartment is sterilized by cleaning it with, or otherwise applying, a suitable disinfectant agent to the surface.
  • a suitable disinfectant agent for instance, a 70% ethanol solution, or some similar alcohol based surface active disinfectant may be used.
  • a suspended bacterial product is added to the bioreactor compartment.
  • the bacterial product is enclosed in a 1/16 inch steel mesh ball, and contains from about 1 to about 15 grams of a nitrifying microorganism composition.
  • the bioreactor is then sealed and all connections are capped off until after installation.
  • the nitrifying and facultative anaerobic de-nitrifying microorganisms employed in the starter material may vary upon the type and concentration of nitrogen-based waste in the environment to be treated.
  • the starter material contains at least one microorganism selected from the group consisting essentially of Nitrosomonas, Nifrosuspira, Nitrosolobus, Nitrococcus. and Nitrobacter.
  • the bacterial product that may be employed contains non-pathogenic microorganisms capable of digesting ammonia and nitrogenous waste found in animal urine and feces.
  • the starter mixture will also contain heterotrophs.
  • the content and concentration of the inorganic and organic nutrients in the food will vary with the type of microorganism used in the apparatus.
  • Media A can be prepared by mixing (NH 4 ) 2 SO ; K 2 HPO ; Fe;MgSO 4 ; CaCl; EDTA; and trace elements that include but are not limited to CuSO4, Co(II)Cl, Molybolic acid, MnCl 2 -7H 2 0, and ZnS0 4 in a mixing kettle. After all ingredients are added the mixture is autoclaved.
  • Media B can be prepared by mixing KHCO 3 ; a sugar source (e.g. dextrose); and an amino acid source (e.g. peptone) in a mixing kettle. After all ingredients are added the mixture is filtered sterilized.
  • a sugar source e.g. dextrose
  • an amino acid source e.g. peptone
  • nitrifying microorganism when the composition is used as a starter material, at least one nitrifying microorganism is added.
  • the nitrifying microorganism may be selected from the group of microorganisms Nitrosomonas. Nitrosuspira- Nitrosolobus, Nitrococcus, and Nitrobacter and may be added prior to inoculation of the bioreactor. It is within the scope of the invention to substitute microorganism not listed that are capable of digesting waste.
  • Both the organic digesting microorganism bioreactor and the nitrifier use potable water in growing and dosing microorganisms to the environment to be treated. It is important to assure that the potable water entering the bioreactors is sterile so as not to contaminant the bioreactors with unwanted microorganisms. Therefore, the portable/mobile wastewater treatment system collects potable water into a reservoir and sterilizes the water before it is pumped to either of the bioreactors.
  • a potable water reservoir and volume regulator provides a regulated acclimatized supply of water for bioreactor.
  • the reservoir and regulator also may operate to dampen external pressure events, isolate the potable water supply, and/or regulate the volume of water provided to the both bioreactor systems through one or more pumps provided by the controller.
  • the potable water reservoir includes a nitrifier and connecting tubes and/or pipes for communication with one or more pump and with the solenoid, and optionally may include a level sensor.
  • the potable water reservoir may also include an air gap for overflow to the atmosphere, and the reservoir and regulator may also be equipped with a water filtration device for preconditioning and/or microbial removal from the source water.
  • the portable/mobile wastewater treatment unit may also include a re-circulating pump.
  • the waste from a pre-determined location within the environment to be treated enters the re-circulating pump through the intake hose that is in fluid communication with the environment to be treated.
  • the waste exits the re-circulating pump at the opposite end into a hose that is in fluid communication with the environment to be treated.
  • the exiting waste is then augmented with oxygen and microorganisms from the dosing tank.
  • circulation of the waste water will help float solids so that the solids are more accessible to the microorganisms and added dissolved oxygen into the lagoon.
  • Adding a restriction nozzle to the effluent flow of the re-circulating pump can increase adding dissolved oxygen.
  • the re-circulating pump is between about 5 to about 15 HP trash pump made by Vaughan Co., Inc. of Montesano, WA or CH & E, Corporation of Milwaukee, Wisconsin, or Gorman Rupp, Corporation of Cleveland Ohio.
  • Figure 1 illustrates a top down view of a cutaway diagram of one embodiment of the invention.
  • Figure 2 illustrates one embodiment of the wheeled container.
  • Figure 3 illustrates one embodiment of the manifold system attached to the portable/mobile wastewater biro-augmentation system.
  • Nitrifier Dosing Tube/Pipe connecting nitrifier with dosing pump
  • the apparatus comprises six main subsystems: a portable/mobile wheeled container (155); a controller unit (175); a bio-augmentation system for growing organic digesting microorganisms; a bio-augmentation system for growing nitrifying microorganisms; potable water reservoir and regulator (135); and a recirculation pump (160).
  • Potable water enters the portable/mobile bio-augmentation unit (10) through water pipe/tube (140) into water reservoir (135).
  • the water reservoir (135) is in fluid communication with bioreactor 1 (50) and bioreactor 2 (120) as well as the nitrifier bioreactor (40).
  • bioreactor 1 (50) and bioreactor 2 (120) are in fluid communication with the nitrifier bioreactor (40).
  • the sterilizers can use ultra- violent light (UV), radiation or any other method of sterilization known in the art.
  • UV ultra- violent light
  • the water enters bioreactor 1 (50) via water pump (130) and bioreactor 2 (120) via water pump (200).
  • the pumps are controlled either by the controller (175) and/or timers (not shown).
  • the composition in bioreactor 1 (50) and bioreactor 2 (120) is displaced through bioreactor 1 dosing tube/pipe (75) and bioreactor 2 dosing tube/pipe (80) into dosing tank (65).
  • the negative suction created from the re-circulating pump (160) draws the microorganism composition out of the tank and dispenses the microorganisms to the environment to be treated.
  • a similar protocol is followed with the dosing of the nitrifier bioreactor (40). Once sterilized, the water enters the nitrifier bioreactor (40) water pump tube/pipe (200) via water pump (130).
  • the pump is controlled either by the controller (175) and/or timers (not shown).
  • the composition in the nitrifier bioreactor (40) is displaced through nitrifier dosing tube/pipe (55) into dosing tank (65). Once the composition from the nitrifier bioreactor (40) comprising activated nitrifying microorganisms enters into the dosing tank the negative suction created from the re-circulating pump (160) draws the microorganism composition out of the tank and dispenses the microorganisms to the environment to be treated.
  • Booster nitrifier innoculant (30) may be refrigerated in refrigerator (25) and periodically dosed into the nitrifier bioreactor (40) via pump (35) according to a predetermined schedule or when the pH of the nitrifier bioreactor (40) dips below a predetermined pH. Either the controller or an independent timer may control the pumps.
  • the nitrifier bioreactor (40) is fed nitrifier nutrients from the nutrient container for the nitrifier bioreactor (20). These nutrients are effective in getting the nitrifying microorganisms into the activated log-growth phase.
  • the nutrients are dispensed to the nitrifier bioreactor (40) according to a predetermined schedule and may be controlled by either the controller or an independent timer.
  • the re-circulation pump (160) has an inflow (145) connected to an intake tube/pipe (150) that supplies the re-circulating pump (160) with liquid from the environment being treated.
  • the re-circulating pump (160) releases the contaminated liquid through the outflow pipe/tube (185) out the outflow nozzle (190) into the environment to be treated.
  • the outflow nozzle (190) may be equipped with a restriction device that increase the amount of dissolved oxygen in the contaminant flow so that additional oxygen is delivered to the environment to be treated aiding in the oxidative digestion of the organic material in the contaminated site.
  • the operation of the re-circulating pump may be connected to the controller (175) that controls the activation and de-activation of the re-circulating pump (160).
  • the portable/mobile wastewater bio- augmentation system may contain a wireless transmitting device (215) that is connected to the controller (175).
  • the controller (175) may be equipped connected to sensors (not shown) dispersed throughout the environment to be treated as well as on the internal components of the portable/mobile wastewater bio-augmentation system (10).
  • the bioreactors may be equipped with a pH sensor that feeds data points to the controller (175) that can be sent to a remote location, cell phone, and or pager when they fall outside of a pre-determined range.
  • the biomass that is released to the environment to be treated may comprise a self- inoculating bio-film.
  • the bio-film released from the bioreactor comprises a highly concentrated number of bacterial colonies that are acclimated to the environment to be treated and are in logarithmic growth phase.
  • a colony should be understood to mean a plurality of bacterial cells aggregated together as a unit compared to the same number of bacterial cells existing independently.
  • the bio-film that is produced in the bioreactor may be produced on a bio-mesh or any other internal surface of the bioreactor.
  • a bio-mesh is a network of filaments aggregated together to provide an increased ratio of surface area to volume.
  • Figure 3 shows one variation of a distributor/delivery manifold system (375) of the present invention.
  • the manifold system configuration may vary according to the configuration of the environment being treated. Although the actual configuration of the manifold system may change, the fundamental components remain the same.
  • the manifold system shown in figure 3 has a floating dock (300) that can be used to take test samples and support a flexible suction hose (330).
  • the flexible suction hose (330) is connected to the portable/mobile bio-augmentation unit (360) at one end, fastened to the floating dock (300) in the middle and terminates in the environment to be treated.
  • the suction hose (330) brings waste from the environment to be treated to the portable/mobile bio-augmentation unit (360) where it is re-circulated out the outflow hose (370) and back into the environment to be treated.
  • the re-circulated waste travels out the outflow hose (370) to the PVC manifold through actuator valves (305) and (310).
  • the actuator valves (305) and (310) can be closed when the unit is not in operation so as to prevent back-ups.
  • the manifold system (375) also has a PVC spray pipe (335) that is attached to several treated posts (365) located at several different points of the environment to be treated. The posts are used for support. At each post the PVC pipe is attached to a big gun Irrigation sprinkler (315) that sprays between 80 and 100 gallons of re-circulated waste per minute in a 90 to about 105 degree radius.
  • the spray pipe (335) is fitted at the end of a section with a PVC Ball Valve (320) that regulates the flow and pressure of the system.
  • the outflow hose (370) is also connected to a sub-surface PVC Pipe (340) that spans at least a portion of the environment to be treated.
  • the sub-surface PVC Pipe (340) is connected to several reducer bushings (350). Connected to each reducer bushing (350) is a PVC distributor pipe (345) that terminates with an eductor nozzle (325).
  • the eductor nozzles (325) mix between 1 to about 5 gallons per minute of re-circulated waste that has been oxygenated and injected with microorganisms from the nitrifier unit and/or bioreactors of the portable/mobile bio-augmentation unit (360) back into the environment to be treated.
  • the sub-surface PVC Pipe (340) is fitted at the end of each section with a PVC Ball Valve (320) that regulates the flow and pressure of the system.
  • manifold system (375) described above is only one design that can be used with the present invention. It is understood that one skilled in the art would be able to design a variation of the manifold to achieve the same basic principle, distributing and delivering the bio-augmentation composition to the environment to be treated. These variations on this basic manifold system are contemplated as being part of the present invention. The exact configuration is not critical to the invention.

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

L'invention concerne un dispositif portable/mobile de recyclage d'eau et de traitement biologique destiné à diffuser des micro-organismes activés dans un environnement à traiter. Cette unité comporte au moins une unité de traitement biologique d'eaux usées, une unité d'aération, une unité de nitrification biologique, une pompe de recyclage destinée à la mise en circulation des eaux usées à traiter, et un dispositif de régulation permettant d'entretenir les conditions du bioréacteur de sorte à maintenir les micro-organismes en phase de croissance exponentielle. L'invention concerne également un procédé de traitement biologique de déchets ainsi qu'une composition nutritive organique et inorganique utilisée pour nourrir les micro-organismes dans le bioréacteur.
PCT/US2003/022524 2002-07-19 2003-07-17 Unite portable/mobile de traitement d'eaux usees Ceased WO2004009496A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003252042A AU2003252042A1 (en) 2002-07-19 2003-07-17 Portable/mobile wastewater treatment unit

Applications Claiming Priority (2)

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US39740402P 2002-07-19 2002-07-19
US60/397,404 2002-07-19

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WO2004009496A3 WO2004009496A3 (fr) 2004-03-04

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2243118A1 (es) * 2003-05-03 2005-11-16 Jose Garcia Gonzalez Producto biologico aplicable para el tratamiento de vertidos residuales y conservacion del agua en espacios naturales.
US7306724B2 (en) 2004-04-23 2007-12-11 Water Standard Co., Llc Wastewater treatment
EP2008975A3 (fr) * 2007-06-18 2009-02-18 Josab Nederland B.V. Procédé pour obtenir de l'eau potable à partir d'une fraction liquide de matières fécales humaines ou animales, unité de bioréacteur, réservoir de stockage, et procédé de croissance d'une culture bactérienne à utiliser dans un tel procédé
EP2070879A1 (fr) * 2007-12-07 2009-06-17 Oy KWH Pipe AB Procédé pour garder actives les boues activées dans une unité d'épuration des eaux usées
ES2397635A1 (es) * 2012-11-27 2013-03-08 Arema, Agua, Residuos Y Medio Ambiente, S.A. Planta compacta para el tratamiento de aguas residuales
WO2014022489A1 (fr) * 2012-07-31 2014-02-06 R N Industries, Inc. Procédés et systèmes de maintien de la température des eaux résiduelles dans un équipement de traitement
WO2016077715A1 (fr) * 2014-11-14 2016-05-19 Plava Pur, Llc Traitement d'eau biologique pour gestion de déchets
WO2019160839A1 (fr) * 2018-02-13 2019-08-22 Rigero Llc Augmentation de production d'eau de surface de source biologique et système de distribution
CN113336322A (zh) * 2021-05-17 2021-09-03 同济大学 一种循环生态厕所的应急启动系统

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997032818A1 (fr) * 1996-03-08 1997-09-12 Environmental Solutions, Inc. Appareil et methode pour l'epuration biologique des dechets
US5928514A (en) * 1996-11-05 1999-07-27 Sea Sanitizer Internation, L.L.C. On board treatment system
US6291233B1 (en) * 1998-09-04 2001-09-18 Anuj K. Saha AGB process for integrated multimedia treatment using bio-sorption
US6402941B1 (en) * 2000-02-07 2002-06-11 Wastewater Biological Solutions, Corp Apparatus for biological treatment of environmental contaminants and waste

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005108309A1 (fr) 2003-05-03 2005-11-17 Garcia Gonzalez Jose Traitement biologique applicable a la biorestauration bacterienne dans des ecosystemes aquatiques et des rejets urbains et industriels pour la digestion adequate de la matiere organique et des nutriments
ES2243118B1 (es) * 2003-05-03 2006-07-16 Jose Garcia Gonzalez Producto biologico aplicable para el tratamiento de vertidos residuales y conservacion del agua en espacios naturales.
ES2243118A1 (es) * 2003-05-03 2005-11-16 Jose Garcia Gonzalez Producto biologico aplicable para el tratamiento de vertidos residuales y conservacion del agua en espacios naturales.
US7306724B2 (en) 2004-04-23 2007-12-11 Water Standard Co., Llc Wastewater treatment
EP2008975A3 (fr) * 2007-06-18 2009-02-18 Josab Nederland B.V. Procédé pour obtenir de l'eau potable à partir d'une fraction liquide de matières fécales humaines ou animales, unité de bioréacteur, réservoir de stockage, et procédé de croissance d'une culture bactérienne à utiliser dans un tel procédé
EP2070879A1 (fr) * 2007-12-07 2009-06-17 Oy KWH Pipe AB Procédé pour garder actives les boues activées dans une unité d'épuration des eaux usées
RU2500626C2 (ru) * 2007-12-07 2013-12-10 Ой Квх Пайп Аб Способ поддержания активированного состояния ила в устройстве для очистки бытовых сточных вод
WO2014022489A1 (fr) * 2012-07-31 2014-02-06 R N Industries, Inc. Procédés et systèmes de maintien de la température des eaux résiduelles dans un équipement de traitement
ES2397635A1 (es) * 2012-11-27 2013-03-08 Arema, Agua, Residuos Y Medio Ambiente, S.A. Planta compacta para el tratamiento de aguas residuales
WO2016077715A1 (fr) * 2014-11-14 2016-05-19 Plava Pur, Llc Traitement d'eau biologique pour gestion de déchets
WO2019160839A1 (fr) * 2018-02-13 2019-08-22 Rigero Llc Augmentation de production d'eau de surface de source biologique et système de distribution
US11840467B2 (en) 2018-02-13 2023-12-12 Rigero Llc Surface water biosource augmentation production and distribution system
US12404191B2 (en) 2018-02-13 2025-09-02 Rigero Llc Surface water biosource augmentation production and distribution system
CN113336322A (zh) * 2021-05-17 2021-09-03 同济大学 一种循环生态厕所的应急启动系统

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AU2003252042A1 (en) 2004-02-09
AU2003252042A8 (en) 2004-02-09

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