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WO2004079370A8 - Analysis of the proteome of a living cell by labelling the proteins in the intact cell - Google Patents

Analysis of the proteome of a living cell by labelling the proteins in the intact cell

Info

Publication number
WO2004079370A8
WO2004079370A8 PCT/NL2004/000157 NL2004000157W WO2004079370A8 WO 2004079370 A8 WO2004079370 A8 WO 2004079370A8 NL 2004000157 W NL2004000157 W NL 2004000157W WO 2004079370 A8 WO2004079370 A8 WO 2004079370A8
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
labeling
cell
intact
analysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/NL2004/000157
Other languages
French (fr)
Other versions
WO2004079370A1 (en
Inventor
Herman Steven Overkleeft
Michiel Alexander Leeuwenburgh
Swieten Paul Franciscus Van
Huib Ovaa
Benedikt Kessler
Hidde Ploegh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universiteit Leiden
Original Assignee
Universiteit Leiden
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universiteit Leiden filed Critical Universiteit Leiden
Publication of WO2004079370A1 publication Critical patent/WO2004079370A1/en
Publication of WO2004079370A8 publication Critical patent/WO2004079370A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

This invention provides a method for the analysis of a protein population of a living cell. It comprises the steps of a) contacting intact cells with a labeling compound comprising a protein-specific irreversible binding element and a reactive functionality Y and subsequently, b) adding a ligation-purification device comprising a purification handle and a reaction partner for said reactive functionality Y, c) purifying by means of said purification handle, and d) analyzing the material obtained from step c. Advantageously in this method the proteins of interest interact with the labeling compound in their actual working environment in an intact living cell. Labeling proteins by contacting intact living cells with a labeling compound, in vivo labeling, is more effective indicating improved conditions and circumstances for binding to proteins of interest in living cells as compared to the in vitro labeling.
PCT/NL2004/000157 2003-03-03 2004-03-03 Analysis of the proteome of a living cell by labelling the proteins in the intact cell Ceased WO2004079370A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP03075642 2003-03-03
EP03075642.3 2003-03-03

Publications (2)

Publication Number Publication Date
WO2004079370A1 WO2004079370A1 (en) 2004-09-16
WO2004079370A8 true WO2004079370A8 (en) 2005-08-04

Family

ID=32946899

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2004/000157 Ceased WO2004079370A1 (en) 2003-03-03 2004-03-03 Analysis of the proteome of a living cell by labelling the proteins in the intact cell

Country Status (1)

Country Link
WO (1) WO2004079370A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9056847B2 (en) 2010-03-10 2015-06-16 Academisch Medisch Centrum Bij Universiteit Van Amsterdam Activity based probes (ABPs) interacting with glycosidases
EP2964610A4 (en) * 2013-03-08 2016-11-23 Univ Southern California Usc Stevens Vinylsulfone-based 18f-labeling compositions and methods and uses thereof
CN114324286B (en) * 2022-01-07 2022-08-02 中国人民解放军军事科学院军事医学研究院 A kind of photosensitive crosslinking agent and its application

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE536422T1 (en) * 1998-08-25 2011-12-15 Univ Washington RAPID QUANTITATIVE ANALYSIS OF PROTEINS OR PROTEIN FUNCTIONS IN COMPLEX MIXTURES
AU2001224349A1 (en) * 2000-04-10 2001-10-23 The Scripps Research Institute Proteomic analysis

Also Published As

Publication number Publication date
WO2004079370A1 (en) 2004-09-16

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Free format text: IN PCT GAZETTE 38/2004 UNDER (72, 75) THE ADDRESS OF "VAN SWIETEN, PAUL, FRANCISCUS" SHOULD READ "KLIKSPAANWEG 46 KAMER 21, NL-2324 LZ LEIDEN (NL)"

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