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WO2004078981A8 - TRANSPOSASES D'ELEMENTS GENETIQUES MOBILES mariner MUTANTES, NON PHOSPHORYLABLES ET HYPERACTIVES - Google Patents

TRANSPOSASES D'ELEMENTS GENETIQUES MOBILES mariner MUTANTES, NON PHOSPHORYLABLES ET HYPERACTIVES

Info

Publication number
WO2004078981A8
WO2004078981A8 PCT/FR2004/000161 FR2004000161W WO2004078981A8 WO 2004078981 A8 WO2004078981 A8 WO 2004078981A8 FR 2004000161 W FR2004000161 W FR 2004000161W WO 2004078981 A8 WO2004078981 A8 WO 2004078981A8
Authority
WO
WIPO (PCT)
Prior art keywords
hyperactive
phosphorylated
genetic elements
transposases
mobile genetic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2004/000161
Other languages
English (en)
Other versions
WO2004078981A1 (fr
Inventor
Yves Bigot
Corinne Auge-Gouillou
Marie-Helene Hamelin
Benjamin Brillet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Universite de Tours
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite Francois Rabelais de Tours
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Universite Francois Rabelais de Tours filed Critical Centre National de la Recherche Scientifique CNRS
Priority to JP2006505659A priority Critical patent/JP2006518220A/ja
Priority to EP04704632A priority patent/EP1590460A1/fr
Priority to CA002514730A priority patent/CA2514730A1/fr
Priority to US10/543,718 priority patent/US7732209B2/en
Publication of WO2004078981A1 publication Critical patent/WO2004078981A1/fr
Anticipated expiration legal-status Critical
Publication of WO2004078981A8 publication Critical patent/WO2004078981A8/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention a pour objets des transposases mutantes, non phosphorylables, hyperactives, d'éléments génétiques mobiles mariner. L'invention concerne également des séquences nucléotidiques recombinantes codant de telles transposases. En outre, l'invention vise un procédé de production de ces transposases, ainsi que l'utilisation de ces dernières pour la transposition in vitro ou in vivo.
PCT/FR2004/000161 2003-01-28 2004-01-23 TRANSPOSASES D'ELEMENTS GENETIQUES MOBILES mariner MUTANTES, NON PHOSPHORYLABLES ET HYPERACTIVES Ceased WO2004078981A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2006505659A JP2006518220A (ja) 2003-01-28 2004-01-23 マリナー可動遺伝因子の突然変異、リン酸化不能および機能亢進性トランスポゼース
EP04704632A EP1590460A1 (fr) 2003-01-28 2004-01-23 Transposases d elements genetiques mobiles mariner mutantes, non phosphorylables et hyperactives
CA002514730A CA2514730A1 (fr) 2003-01-28 2004-01-23 Transposases d'elements genetiques mobiles mariner mutantes, non phosphorylables et hyperactives
US10/543,718 US7732209B2 (en) 2003-01-28 2004-01-23 Hyperactive, non-phosphorylated, mutant transposases of mariner mobile genetic elements

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR03/00905 2003-01-28
FR0300905A FR2850395B1 (fr) 2003-01-28 2003-01-28 Transposases d'elements genetiques mobiles mariner mutantes, non phosphorylables et hyperactives

Publications (2)

Publication Number Publication Date
WO2004078981A1 WO2004078981A1 (fr) 2004-09-16
WO2004078981A8 true WO2004078981A8 (fr) 2005-09-09

Family

ID=32669262

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2004/000161 Ceased WO2004078981A1 (fr) 2003-01-28 2004-01-23 TRANSPOSASES D'ELEMENTS GENETIQUES MOBILES mariner MUTANTES, NON PHOSPHORYLABLES ET HYPERACTIVES

Country Status (6)

Country Link
US (1) US7732209B2 (fr)
EP (1) EP1590460A1 (fr)
JP (1) JP2006518220A (fr)
CA (1) CA2514730A1 (fr)
FR (1) FR2850395B1 (fr)
WO (1) WO2004078981A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2893951B1 (fr) * 2005-11-30 2008-02-29 Centre Nat Rech Scient Procede de production chez les procaryotes de transposases actives et stables d'elements genetiques mobiles mariner
FR2900935A1 (fr) * 2006-05-15 2007-11-16 Centre Nat Rech Scient Systemes de transposition recombinants hyperactifs derives du transposon mos-1
ES2334087B1 (es) * 2008-02-29 2011-01-27 Universidad De Jaen Vectores y usos del transposon mboumar.
US8399643B2 (en) 2009-02-26 2013-03-19 Transposagen Biopharmaceuticals, Inc. Nucleic acids encoding hyperactive PiggyBac transposases
CN103627684B (zh) * 2013-11-20 2016-07-06 浙江农林大学 人工优化的高活性Mariner-Like转座酶

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2395094A3 (fr) * 1999-06-01 2012-04-18 The University of Utah Research Foundation Procédé de mutagénèse par transposon dans le nématode caenorhabditis elegans
CA2319168A1 (fr) * 1999-10-01 2001-04-01 The Board Of Trustees Of The University Of Illinois Mutants hyperactifs de la transposase himar1 et methodes pour leur utilisation
CN1300827A (zh) * 1999-12-22 2001-06-27 上海博德基因开发有限公司 一种新的多肽—人水手转座酶19和编码这种多肽的多核苷酸

Also Published As

Publication number Publication date
JP2006518220A (ja) 2006-08-10
CA2514730A1 (fr) 2004-09-16
FR2850395B1 (fr) 2007-12-14
US7732209B2 (en) 2010-06-08
EP1590460A1 (fr) 2005-11-02
US20070031967A1 (en) 2007-02-08
WO2004078981A1 (fr) 2004-09-16
FR2850395A1 (fr) 2004-07-30

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