[go: up one dir, main page]

WO2004067745A8 - Proteine de fusion inductrice de l’expression d’un gene et procede de controle de l’induction de l’expression d’un gene - Google Patents

Proteine de fusion inductrice de l’expression d’un gene et procede de controle de l’induction de l’expression d’un gene

Info

Publication number
WO2004067745A8
WO2004067745A8 PCT/FR2004/000073 FR2004000073W WO2004067745A8 WO 2004067745 A8 WO2004067745 A8 WO 2004067745A8 FR 2004000073 W FR2004000073 W FR 2004000073W WO 2004067745 A8 WO2004067745 A8 WO 2004067745A8
Authority
WO
WIPO (PCT)
Prior art keywords
gene expression
fusion protein
inducing fusion
induction
controlling
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2004/000073
Other languages
English (en)
Other versions
WO2004067745A1 (fr
Inventor
Christel Boutonnet
Stephan Vagner
Jean-Charles Faye
Gilles Favre
Abdelhakim Kharrat
Khalil Bouayadi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut National de la Sante et de la Recherche Medicale INSERM
MilleGen SA
Universite de Toulouse
Original Assignee
Institut National de la Sante et de la Recherche Medicale INSERM
MilleGen SA
Universite Toulouse III Paul Sabatier
Universite de Toulouse
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut National de la Sante et de la Recherche Medicale INSERM, MilleGen SA, Universite Toulouse III Paul Sabatier, Universite de Toulouse filed Critical Institut National de la Sante et de la Recherche Medicale INSERM
Priority to US10/542,471 priority Critical patent/US20060134738A1/en
Priority to EP04702354A priority patent/EP1583834A1/fr
Publication of WO2004067745A1 publication Critical patent/WO2004067745A1/fr
Anticipated expiration legal-status Critical
Publication of WO2004067745A8 publication Critical patent/WO2004067745A8/fr
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
    • A61K48/0025—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition wherein the non-active part clearly interacts with the delivered nucleic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
    • A61K48/0025—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition wherein the non-active part clearly interacts with the delivered nucleic acid
    • A61K48/0041—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition wherein the non-active part clearly interacts with the delivered nucleic acid the non-active part being polymeric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

L'invention a pour objet une protéine de fusion inductrice de l'expression d'un gène comprenant, d'une part, un domaine peptidique de liaison aux acides ribonucléiques et un domaine activateur de l'expression post-transcriptionnelle du gène, et, d'autre part, un domaine permettant une délocalisation à la membrane cytoplasmique. L'invention a également pour objet un procédé de contrôle externe permanent et modulable de l'induction de l'expression d'un gène par modulation de l'état de modification post-traductionnelle de la protéine de fusion inductrice de l'expression dudit gène.
PCT/FR2004/000073 2003-01-15 2004-01-15 Proteine de fusion inductrice de l’expression d’un gene et procede de controle de l’induction de l’expression d’un gene Ceased WO2004067745A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/542,471 US20060134738A1 (en) 2003-01-15 2004-01-15 Gene expression inducing fusion protein and method for controlling gene expression Induction
EP04702354A EP1583834A1 (fr) 2003-01-15 2004-01-15 PROTEINE DE FUSION INDUCTRICE DE L'EXPRESSION D'UN GENE ET PROCEDE DE CONTROLE DE L’INDUCTION DE L'EXPRESSION D'UN GENE

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR03/00422 2003-01-15
FR0300422A FR2849853A1 (fr) 2003-01-15 2003-01-15 Proteine de fusion inductrice de l'expression d'un gene et procede de controle de l'induction de l'expression d'un gene

Publications (2)

Publication Number Publication Date
WO2004067745A1 WO2004067745A1 (fr) 2004-08-12
WO2004067745A8 true WO2004067745A8 (fr) 2005-09-09

Family

ID=32524949

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2004/000073 Ceased WO2004067745A1 (fr) 2003-01-15 2004-01-15 Proteine de fusion inductrice de l’expression d’un gene et procede de controle de l’induction de l’expression d’un gene

Country Status (4)

Country Link
US (1) US20060134738A1 (fr)
EP (1) EP1583834A1 (fr)
FR (1) FR2849853A1 (fr)
WO (1) WO2004067745A1 (fr)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9226065D0 (en) * 1992-12-14 1993-02-10 Ici Plc Peptides
GB9718591D0 (en) * 1997-09-03 1997-11-05 Zeneca Ltd Methods
US6610508B1 (en) * 1999-03-08 2003-08-26 Anadys Pharmaceuticals, Inc. Translation driver system and methods for use thereof

Also Published As

Publication number Publication date
FR2849853A1 (fr) 2004-07-16
WO2004067745A1 (fr) 2004-08-12
US20060134738A1 (en) 2006-06-22
EP1583834A1 (fr) 2005-10-12

Similar Documents

Publication Publication Date Title
AU2003239365A1 (en) Recombinant glycosyltransferase fusion proteins
WO2002074928A3 (fr) Peptides de fusion pouvant etre isoles par transition de phase
EP2484774A3 (fr) Expression de gènes multiples incluant des constructions molles avec des polyprotéines, des pro-polyprotéines et protéolyse
DE60041958D1 (de) Selektion von proteinen unter verwendung von rna-protein-fusionen
WO2006036916A3 (fr) Modulation d'arni d'apob et utilisations correspondantes
AU2003237875A1 (en) Antisense modulation of apolipoprotein b expression
AU2003229013A1 (en) Modulation scheme for driving digital display systems
TW200734685A (en) Control of electrowetting lenses
WO2005047459A3 (fr) Acides nucleiques du sars, proteines, anticorps et utilisations associees
WO2003096981A3 (fr) Procede de modulation de l'angiogenese
AU2003206264A1 (en) MODULATING TOLERANCE BY MODULATING FcGammaRIIB RECEPTOR SIGNALLING
AU2003252072A1 (en) Modulation of protein kinase c-iota expression
WO2006113475A3 (fr) Procedes et compositions pour moduler l'adhesion et la resistance au stress chez des bacteries
AU2003257536A1 (en) Method of stabilizing protein solution preparation
AU2003298523A1 (en) Methods for efficient production of mammalian recombinant proteins
WO2004067745A8 (fr) Proteine de fusion inductrice de l’expression d’un gene et procede de controle de l’induction de l’expression d’un gene
BRPI0516576C8 (pt) Vetores quiméricos
EP1695069A4 (fr) Systeme de surveillance de sulfure d'hydrogene
DE60040524D1 (de) Intein-vermittelte ligation von exprimierten proteinen
AU2003254680A1 (en) Staphylococcal nuclease fusion proteins for the production of recombinant peptides
AU2003226591A1 (en) Telecommunication system with message based control
WO2000068416A3 (fr) Procedes pour la recherche de proteases separant des substrats lies par membrane de maniere specifique
WO2002081695A3 (fr) Genes modules par extinction post-transcriptionnelle de l'expression genique
AU2003295790A1 (en) Modulation of iap-like expression
AU2003295794A1 (en) Modulation of stat2 expression

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): BW GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2004702354

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2006134738

Country of ref document: US

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 10542471

Country of ref document: US

CFP Corrected version of a pamphlet front page
CR1 Correction of entry in section i

Free format text: IN PCT GAZETTE 33/2004 UNDER (71) (FOR US ONLY) DELETE "INSERM [FR/FR] 101, RUE DE TOLBIAC, F-75654PARIS CEDEX 13 (FR)"; UNDER (71) (FOR ALL DESIGNATED STATES EXCEPT THE US) ADD "INSERM [FR/FR] 101, RUE DE TOLBIAC, F-75654 PARIS CEDEX 13 (FR)."

WWP Wipo information: published in national office

Ref document number: 2004702354

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2004702354

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 10542471

Country of ref document: US