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WO2004063359A3 - Verfahren zur herstellung von carotinoiden oder deren vorstufen mittels gentechnisch veränderter organismen der gattung blakeslea, mit dem verfahren hergestellte carotinoide oder deren vorstufen und deren verwendung - Google Patents

Verfahren zur herstellung von carotinoiden oder deren vorstufen mittels gentechnisch veränderter organismen der gattung blakeslea, mit dem verfahren hergestellte carotinoide oder deren vorstufen und deren verwendung Download PDF

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Publication number
WO2004063359A3
WO2004063359A3 PCT/EP2004/000099 EP2004000099W WO2004063359A3 WO 2004063359 A3 WO2004063359 A3 WO 2004063359A3 EP 2004000099 W EP2004000099 W EP 2004000099W WO 2004063359 A3 WO2004063359 A3 WO 2004063359A3
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WIPO (PCT)
Prior art keywords
carotenoids
cells
genetically modified
precursors
produced
Prior art date
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Ceased
Application number
PCT/EP2004/000099
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English (en)
French (fr)
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WO2004063359A2 (de
Inventor
Markus Matuschek
Daniela Klein
Thorsten Heinekamp
Andre Schmidt
Axel Brakhage
Brigitte Achatz
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BASF SE
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BASF SE
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Priority claimed from DE10300649A external-priority patent/DE10300649A1/de
Priority claimed from DE10341271A external-priority patent/DE10341271A1/de
Application filed by BASF SE filed Critical BASF SE
Priority to EP04700978A priority Critical patent/EP1592783A2/de
Priority to US10/541,750 priority patent/US20060234333A1/en
Priority to JP2005518516A priority patent/JP2006515516A/ja
Publication of WO2004063359A2 publication Critical patent/WO2004063359A2/de
Publication of WO2004063359A3 publication Critical patent/WO2004063359A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/179—Colouring agents, e.g. pigmenting or dyeing agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/40—Colouring or decolouring of foods
    • A23L5/42—Addition of dyes or pigments, e.g. in combination with optical brighteners
    • A23L5/43—Addition of dyes or pigments, e.g. in combination with optical brighteners using naturally occurring organic dyes or pigments, their artificial duplicates or their derivatives
    • A23L5/44—Addition of dyes or pigments, e.g. in combination with optical brighteners using naturally occurring organic dyes or pigments, their artificial duplicates or their derivatives using carotenoids or xanthophylls
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/02—Nutrients, e.g. vitamins, minerals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Husbandry (AREA)
  • Diabetes (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Verfahren zur Herstellung von Carotinoiden oder deren Vorstufen mittels gentechnisch veränderter Organismen der Gattung Blakeslea umfassend (i) Transformation mindestens einer der Zellen, (ii) ggf. Homokaryotisierung der aus (i) erhaltenen Zellen, so dass Zellen entstehen, in denen die Kerne in einem oder in mehreren genetischen Merkmalen alle gleichartig verändert sind und diese genetische Veränderung zur Ausprägung bringen, und (ix) Selektion und Vermehrung der gentechnisch veränderten Zelle oder Zellen, (x) Kultivierung der gentechnisch veränderten Zellen, (xi) Bereitstellung des von den gentechnisch veränderten Zellen produzierten Carotinoids oder der von den gentechnischen veränderten Zellen produzierten Carotinoidvorstufe; nach dem Verfahren hergestellte Carotinoide oder deren Vorstufen und deren Verwendung.
PCT/EP2004/000099 2003-01-09 2004-01-09 Verfahren zur herstellung von carotinoiden oder deren vorstufen mittels gentechnisch veränderter organismen der gattung blakeslea, mit dem verfahren hergestellte carotinoide oder deren vorstufen und deren verwendung Ceased WO2004063359A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP04700978A EP1592783A2 (de) 2003-01-09 2004-01-09 Verfahren zur herstellung von carotinoiden oder deren vorstufen mittels gentechnisch veränderter organismen der gattung blakeslea, mit dem verfahren hergestellte carotinoide oder deren vorstufen und deren verwendung
US10/541,750 US20060234333A1 (en) 2003-01-09 2004-01-09 Method for producing carotenoids or their precursors using genetically modified organisms of the blakeslea genus, carotenoids or their precursors produced by said method and use thereof
JP2005518516A JP2006515516A (ja) 2003-01-09 2004-01-09 遺伝子改変されたＢｌａｋｅｓｌｅａ属の生物を使用する、カロチノイドまたはそれらの前駆体を製造するための方法、上記方法によって製造されたカロチノイドまたはそれらの前駆体、ならびにそれらの使用

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10300649A DE10300649A1 (de) 2003-01-09 2003-01-09 Verfahren zur Herstellung von Ketocarotinoiden durch Kultivierung von genetisch veränderten Organismen
DE10300649.4 2003-01-09
DE10341271.9 2003-09-08
DE10341271A DE10341271A1 (de) 2003-09-08 2003-09-08 Verfahren zur Herstellung von Carotinoiden oder deren Vorstufen mittels gentechnisch veränderter Organismen der Gattung Blakeslea, mit dem Verfahren hergestellte Carotinoide oder deren Vorstufen und deren Verwendung

Publications (2)

Publication Number Publication Date
WO2004063359A2 WO2004063359A2 (de) 2004-07-29
WO2004063359A3 true WO2004063359A3 (de) 2005-01-27

Family

ID=32714777

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/000099 Ceased WO2004063359A2 (de) 2003-01-09 2004-01-09 Verfahren zur herstellung von carotinoiden oder deren vorstufen mittels gentechnisch veränderter organismen der gattung blakeslea, mit dem verfahren hergestellte carotinoide oder deren vorstufen und deren verwendung

Country Status (6)

Country Link
US (1) US20060234333A1 (de)
EP (1) EP1592783A2 (de)
JP (1) JP2006515516A (de)
KR (1) KR20050092739A (de)
RU (1) RU2005125072A (de)
WO (1) WO2004063359A2 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi

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US7663021B2 (en) 2002-12-06 2010-02-16 Del Monte Fresh Produce Company Transgenic pineapple plants with modified carotenoid levels and methods of their production
WO2004074490A2 (en) * 2003-02-24 2004-09-02 Genoclipp Biotechnology B.V. Method for transforming blakeslea strains
DE102004007624A1 (de) * 2004-02-17 2005-09-15 Sungene Gmbh & Co. Kgaa Neue Ketolasen und Verfahren zur Herstellung von Ketocarotinoiden
US7091031B2 (en) * 2004-08-16 2006-08-15 E. I. Du Pont De Nemours And Company Carotenoid hydroxylase enzymes
EP1696032A1 (de) * 2005-02-23 2006-08-30 Bayer CropScience GmbH Verfahren und Mittel zur Herstellung von Hyaluronan in Pilzen
CA2602183C (en) 2005-03-18 2014-05-06 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
JP5706056B2 (ja) * 2006-10-17 2015-04-22 Ｊｘ日鉱日石エネルギー株式会社 サケ類の肉色改善方法
AU2008229076B2 (en) 2007-03-16 2014-05-15 Genomatica, Inc. Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors
JP4969370B2 (ja) * 2007-08-29 2012-07-04 Ｊｘ日鉱日石エネルギー株式会社 カロテノイドの製造方法
JP5762691B2 (ja) * 2010-03-15 2015-08-12 Ｊｘ日鉱日石エネルギー株式会社 発酵によるアスタキサンチン製造方法
EP2571996B1 (de) 2010-05-17 2017-03-08 Dynadis Biotehc (India) Private Limited Verfahren zur herstellung von hochreinem beta-karotin und lycopenkristallen aus pilzbiomasse
CN113774034A (zh) * 2012-12-20 2021-12-10 帝斯曼知识产权资产管理有限公司 胡萝卜素羟化酶及其用于产生类胡萝卜素的用途
US10125104B2 (en) * 2014-05-20 2018-11-13 Asta Pharmaceuticals Co., Ltd. Carotenoid derivative, pharmaceutically acceptable salt thereof, or pharmaceutically acceptable ester or amide thereof
KR101631057B1 (ko) * 2014-08-22 2016-06-17 영남대학교 산학협력단 발효된 감 슬러지를 이용한 카로티노이드의 생산방법
US11229095B2 (en) 2014-12-17 2022-01-18 Campbell Soup Company Electromagnetic wave food processing system and methods
EP3555302A1 (de) * 2016-12-16 2019-10-23 Deinove Verfahren zur herstellung von phytoen
WO2019222711A1 (en) 2018-05-17 2019-11-21 Lumen Bioscience, Inc. Arthrospira platensis oral vaccine delivery platform
US12252513B2 (en) 2018-07-16 2025-03-18 Lumen Bioscience, Inc. Thermostable phycobiliproteins produced from recombinant arthrospira
CN108893486A (zh) * 2018-08-01 2018-11-27 四川省农业科学院经济作物育种栽培研究所 一种可用于丝状真菌基因敲除的载体及应用
NL2026526B1 (en) * 2020-09-23 2022-05-24 Grassa B V Method, extraction system, and use of said system for extracting proteins from leaf juice
CN112226376A (zh) * 2020-09-25 2021-01-15 西北农林科技大学 酿造酵母Bei-29的健康营养型果酒的制备方法及检测方法
IT202200015231A1 (it) * 2022-07-20 2024-01-20 Bioinnova S R L S Microalghe esprimenti prodotti biologicamente attivi

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WO1998003480A1 (en) * 1996-07-19 1998-01-29 Gist-Brocade B.V. PROCESS FOR THE RECOVERY OF CRYSTALLINE β-CAROTENE FROM A NATURAL SOURCE
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WO1998050574A1 (en) * 1997-05-02 1998-11-12 Dsm N.V. Isolation of carotenoid crystals from microbial biomass
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WO1998003480A1 (en) * 1996-07-19 1998-01-29 Gist-Brocade B.V. PROCESS FOR THE RECOVERY OF CRYSTALLINE β-CAROTENE FROM A NATURAL SOURCE
WO1998046772A2 (en) * 1997-04-11 1998-10-22 Dsm N.V. Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
WO1998050574A1 (en) * 1997-05-02 1998-11-12 Dsm N.V. Isolation of carotenoid crystals from microbial biomass
US20020051998A1 (en) * 1999-12-08 2002-05-02 California Institute Of Technology Directed evolution of biosynthetic and biodegradation pathways

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
US9297031B2 (en) 2006-09-28 2016-03-29 Dsm Ip Assets B.V. Production of carotenoids in oleaginous yeast and fungi

Also Published As

Publication number Publication date
WO2004063359A2 (de) 2004-07-29
KR20050092739A (ko) 2005-09-22
US20060234333A1 (en) 2006-10-19
JP2006515516A (ja) 2006-06-01
RU2005125072A (ru) 2006-06-10
EP1592783A2 (de) 2005-11-09

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