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WO2004061126A3 - Nucleic acid hybridization assay using bridging sequence - Google Patents

Nucleic acid hybridization assay using bridging sequence Download PDF

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Publication number
WO2004061126A3
WO2004061126A3 PCT/US2003/009232 US0309232W WO2004061126A3 WO 2004061126 A3 WO2004061126 A3 WO 2004061126A3 US 0309232 W US0309232 W US 0309232W WO 2004061126 A3 WO2004061126 A3 WO 2004061126A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
sequence
acid hybridization
hybridization assay
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/009232
Other languages
French (fr)
Other versions
WO2004061126A2 (en
Inventor
Robert C Getts
James Kadushin
Jaime Schwalm
Kevin Howerton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Datascope Investment Corp
Original Assignee
Datascope Investment Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Datascope Investment Corp filed Critical Datascope Investment Corp
Priority to AU2003230736A priority Critical patent/AU2003230736A1/en
Publication of WO2004061126A2 publication Critical patent/WO2004061126A2/en
Anticipated expiration legal-status Critical
Priority to US10/951,549 priority patent/US20050202461A1/en
Publication of WO2004061126A3 publication Critical patent/WO2004061126A3/en
Priority to US11/584,751 priority patent/US20080033159A1/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods for assay and/or amplification of nucleic acid sequences. An amplification procedure is provided for use with RNA samples that do not have a poly A sequence on the 3 prime end of the messenger RNA as is the case for bacterial, and other total RNA sources. Further, a method is provided for coupling, via ligation, a nucleic acid sequence to the 5 prime end of a random or sequence specific primer or to the 3 prime or 5 prime end of a synthesized DNA probe or target sequence, preferably to enable labelling of the target sequence or amplification thereof.
PCT/US2003/009232 2000-03-08 2003-03-25 Nucleic acid hybridization assay using bridging sequence Ceased WO2004061126A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2003230736A AU2003230736A1 (en) 2002-03-25 2003-03-25 Nucleic acid hybridization assay using bridging sequence
US10/951,549 US20050202461A1 (en) 2000-03-08 2004-09-27 Method for converting generic nucleic acid priming sequences
US11/584,751 US20080033159A1 (en) 2001-01-13 2006-10-20 Method for small volume nucleic acid synthesis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36743802P 2002-03-25 2002-03-25
US60/367,438 2002-03-25

Related Child Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2004/031804 Continuation-In-Part WO2005030984A2 (en) 2001-01-13 2004-09-27 Method for small volume nucleic acid synthesis
US10/951,549 Continuation US20050202461A1 (en) 2000-03-08 2004-09-27 Method for converting generic nucleic acid priming sequences

Publications (2)

Publication Number Publication Date
WO2004061126A2 WO2004061126A2 (en) 2004-07-22
WO2004061126A3 true WO2004061126A3 (en) 2005-05-26

Family

ID=32712861

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/009232 Ceased WO2004061126A2 (en) 2000-03-08 2003-03-25 Nucleic acid hybridization assay using bridging sequence

Country Status (2)

Country Link
AU (1) AU2003230736A1 (en)
WO (1) WO2004061126A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1835026A4 (en) * 2004-10-14 2009-11-04 Univ Nihon HYBRID PRIMER TO A PROMOTER REGION
EP4644543A1 (en) * 2022-12-30 2025-11-05 BGI Shenzhen Splint sequence and use thereof in nucleic acid capture

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5627030A (en) * 1994-06-28 1997-05-06 Kalyx Biosciences Inc. Method of amplification for increasing the sensitivity of detecting nucleic acid-probe target hybrids
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5627030A (en) * 1994-06-28 1997-05-06 Kalyx Biosciences Inc. Method of amplification for increasing the sensitivity of detecting nucleic acid-probe target hybrids
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support

Also Published As

Publication number Publication date
AU2003230736A1 (en) 2004-07-29
AU2003230736A8 (en) 2004-07-29
WO2004061126A2 (en) 2004-07-22

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