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WO2004056964A3 - Vectors for inducible rna interference - Google Patents

Vectors for inducible rna interference Download PDF

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Publication number
WO2004056964A3
WO2004056964A3 PCT/US2003/040548 US0340548W WO2004056964A3 WO 2004056964 A3 WO2004056964 A3 WO 2004056964A3 US 0340548 W US0340548 W US 0340548W WO 2004056964 A3 WO2004056964 A3 WO 2004056964A3
Authority
WO
WIPO (PCT)
Prior art keywords
vectors
rna interference
inducible rna
inducible
interfere
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/040548
Other languages
French (fr)
Other versions
WO2004056964A2 (en
Inventor
William Winston
Ronan C O'hagan
Joerg Heyer
William Rideout
Bijan Etemad-Moghadam
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aveo Pharmaceuticals Inc
Original Assignee
Genpath Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genpath Pharmaceuticals Inc filed Critical Genpath Pharmaceuticals Inc
Priority to AU2003299732A priority Critical patent/AU2003299732A1/en
Publication of WO2004056964A2 publication Critical patent/WO2004056964A2/en
Publication of WO2004056964A3 publication Critical patent/WO2004056964A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Recombinant vectors for inducibly expressing double-stranded RNA molecules that interfere with the expression of a target gene.
PCT/US2003/040548 2002-12-18 2003-12-18 Vectors for inducible rna interference Ceased WO2004056964A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003299732A AU2003299732A1 (en) 2002-12-18 2003-12-18 Vectors for inducible rna interference

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US43485602P 2002-12-18 2002-12-18
US60/434,856 2002-12-18
US49931303P 2003-08-28 2003-08-28
US60/499,313 2003-08-28

Publications (2)

Publication Number Publication Date
WO2004056964A2 WO2004056964A2 (en) 2004-07-08
WO2004056964A3 true WO2004056964A3 (en) 2004-10-14

Family

ID=32685340

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/040548 Ceased WO2004056964A2 (en) 2002-12-18 2003-12-18 Vectors for inducible rna interference

Country Status (2)

Country Link
AU (1) AU2003299732A1 (en)
WO (1) WO2004056964A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8323902B2 (en) 2004-12-22 2012-12-04 Genentech, Inc. Methods for producing soluble membrane-spanning proteins

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004065613A2 (en) * 2003-01-17 2004-08-05 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Inducible small interfering rna (sirna) expression constructs for targeted gene silencing
FR2877674A1 (en) * 2004-11-08 2006-05-12 Genoxay Sa MODEL ANIMALS COMPRISING AT LEAST ONE TRANSGENE AND A SEQUENCE PERMITTING CONTROLLED EXPRESSION OF AN INTERFERING RNA WITH SAID TRANSGENE
EP1731607A1 (en) * 2005-06-09 2006-12-13 ARTEMIS Pharmaceuticals GmbH shRNA and siRNA expression in a living organism under control of a codon-optimized tetracycline repressor gene
US20090210955A1 (en) * 2005-06-09 2009-08-20 Artemis Pharmaceuticals Gmbh Shrna and sirna expression in a living organism under control of a codon-optimized repressor gene
WO2007014363A2 (en) * 2005-07-27 2007-02-01 Genentech, Inc. Vectors for inducible expression of hairpin rna and use thereof
US20070113295A1 (en) * 2005-09-23 2007-05-17 California Institute Of Technology Gene blocking method
GB0602173D0 (en) 2006-02-03 2006-03-15 Avecia Ltd Expression system
EP3219799A1 (en) * 2016-03-17 2017-09-20 IMBA-Institut für Molekulare Biotechnologie GmbH Conditional crispr sgrna expression
CN110540995B (en) * 2018-05-28 2023-01-10 南京农业大学 A protein that reduces myosin-5 and its application in the management of cyanoacrylate drug resistance

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5917122A (en) * 1992-08-26 1999-06-29 Byrne; Guerard Tetracycline repressor-mediated binary regulation system for control of gene expression in transgenic mice
US20040002077A1 (en) * 2001-11-28 2004-01-01 Center For Advanced Science And Technology Incubation, Ltd. siRNA expression system and method for producing functional gene knock-down cell using the system
US20040005593A1 (en) * 2002-03-06 2004-01-08 Rigel Pharmaceuticals, Inc. Novel method for delivery and intracellular synthesis of siRNA molecules
US20040115815A1 (en) * 2002-07-24 2004-06-17 Immusol, Inc. Single promoter system for making siRNA expression cassettes and expression libraries using a polymerase primer hairpin linker

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5917122A (en) * 1992-08-26 1999-06-29 Byrne; Guerard Tetracycline repressor-mediated binary regulation system for control of gene expression in transgenic mice
US20040002077A1 (en) * 2001-11-28 2004-01-01 Center For Advanced Science And Technology Incubation, Ltd. siRNA expression system and method for producing functional gene knock-down cell using the system
US20040005593A1 (en) * 2002-03-06 2004-01-08 Rigel Pharmaceuticals, Inc. Novel method for delivery and intracellular synthesis of siRNA molecules
US20040115815A1 (en) * 2002-07-24 2004-06-17 Immusol, Inc. Single promoter system for making siRNA expression cassettes and expression libraries using a polymerase primer hairpin linker

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
CZAUDERNA FRANK; ET AL: "Inducible shRNA expression for application in a prostate cancer mouse model", NUCLEIC ACIDS RESEARCH, vol. 31, no. 21, 1 November 2003 (2003-11-01), pages E127-1 - E127-7, XP002291076 *
ELBASHIR S.M. ET AL: "Analysis of gene function in somatic mammalian cells using small interfering RNAs", METHODS, vol. 26, no. 2, February 2002 (2002-02-01), pages 199 - 213, XP002251055 *
HANNON G.J.: "RNA interference", NATURE, vol. 418, 11 July 2002 (2002-07-11), pages 244 - 251, XP002979088 *
HASUWA H. ET AL: "Small interfering RNA and gene silencing in transgenic mice and rats", FEBS LETTERS, vol. 532, 13 November 2002 (2002-11-13), pages 227 - 230, XP004395375 *
OGUETA ET AL: "Design and in vitro characterization of a single regulatory module for efficient control of gene expression in both plasmid DNA and a self-inactivating lentiviral vector", MOLECULAR MEDICINE, vol. 7, no. 8, August 2001 (2001-08-01), pages 569 - 579, XP002960124 *
OHKAWA ET AL: "Control of the functional activity of an antisense RNA by a tetracyclin-responsive derivative of the human U6 snRNA promoter", HUMAN GENE THERAPY, vol. 11, no. 4, 1 March 2000 (2000-03-01), pages 577 - 585, XP000926522 *
SASAKI ET AL: "A system for conditional RNA interference in the mouse using the lac operator - repressor system", SOCIETY FOR NEUROSCIENCE ABSTRACT VIEWER AND ITINERARY PLANNER, vol. 2003, no. ABSTRACT NO. 325.4, 8 November 2003 (2003-11-08), XP002979518 *
SUI ET AL: "A DNA vector-based RNAi technology to suppress gene expression in mammalian cells", PNAS, vol. 99, no. 8, 16 April 2002 (2002-04-16), pages 5515 - 5520, XP002964701 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8323902B2 (en) 2004-12-22 2012-12-04 Genentech, Inc. Methods for producing soluble membrane-spanning proteins

Also Published As

Publication number Publication date
AU2003299732A1 (en) 2004-07-14
AU2003299732A8 (en) 2004-07-14
WO2004056964A2 (en) 2004-07-08

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