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WO2004053137A3 - Method for protein production - Google Patents

Method for protein production Download PDF

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Publication number
WO2004053137A3
WO2004053137A3 PCT/CH2003/000810 CH0300810W WO2004053137A3 WO 2004053137 A3 WO2004053137 A3 WO 2004053137A3 CH 0300810 W CH0300810 W CH 0300810W WO 2004053137 A3 WO2004053137 A3 WO 2004053137A3
Authority
WO
WIPO (PCT)
Prior art keywords
production
host cells
recombinant polypeptides
present
rna molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CH2003/000810
Other languages
French (fr)
Other versions
WO2004053137A2 (en
Inventor
Philippe Saudan
Frank Hennecke
Martin F Bachmann
Dorothee M Stern
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytos Biotechnology AG
Original Assignee
Cytos Biotechnology AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cytos Biotechnology AG filed Critical Cytos Biotechnology AG
Priority to AU2003283174A priority Critical patent/AU2003283174A1/en
Publication of WO2004053137A2 publication Critical patent/WO2004053137A2/en
Publication of WO2004053137A3 publication Critical patent/WO2004053137A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
    • C12N2710/16641—Use of virus, viral particle or viral elements as a vector
    • C12N2710/16643—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to the field of gene expression technology and the production of recombinant polypeptides and/or untranslated RNA molecules in host cells. In particular, the invention provides a method for the production of recombinant polypeptides and/or untranslated RNA molecules in host cells allowing the production of high amounts of those molecules in a very short time and with a high degree of reproducibility, in particular with respect to a, preferably, pre-defined standard and, thus, making the present invention particularly attractive for the production of proteins for clinical use and the production under Good Manufacturing Practice (GMP).
PCT/CH2003/000810 2002-12-11 2003-12-10 Method for protein production Ceased WO2004053137A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003283174A AU2003283174A1 (en) 2002-12-11 2003-12-10 Method for protein production

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43224502P 2002-12-11 2002-12-11
US60/432,245 2002-12-11

Publications (2)

Publication Number Publication Date
WO2004053137A2 WO2004053137A2 (en) 2004-06-24
WO2004053137A3 true WO2004053137A3 (en) 2004-08-12

Family

ID=32507877

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CH2003/000810 Ceased WO2004053137A2 (en) 2002-12-11 2003-12-10 Method for protein production

Country Status (2)

Country Link
AU (1) AU2003283174A1 (en)
WO (1) WO2004053137A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7371542B2 (en) 2003-07-11 2008-05-13 Cytos Biotechnology Ag Gene expression system

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002074969A2 (en) 2001-01-26 2002-09-26 University Of Lausanne Matrix attachment regions and methods for use thereof
DE10333675A1 (en) * 2003-07-24 2005-03-03 Aventis Pharma Deutschland Gmbh Perfusion method for the production of erythropoietin
US8252917B2 (en) 2003-10-24 2012-08-28 Selexis S.A. High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences
JP2006121995A (en) * 2004-10-29 2006-05-18 Tokyo Medical & Dental Univ EBNA1 mutant
WO2006060769A2 (en) * 2004-12-03 2006-06-08 Xoma Technology Ltd. Methods and materials for expression of a recombinant protein
WO2007048601A1 (en) 2005-10-28 2007-05-03 F. Hoffmann-La Roche Ag Protein expression in rodent cells
US20100183558A1 (en) 2008-10-17 2010-07-22 Zhennan Lai Safe lentiviral vectors for targeted delivery of multiple therapeutic molecules
US20180161455A1 (en) 2015-06-10 2018-06-14 American Gene Technologies International, Inc. Non-integrating viral delivery system and methods of use therof
US11980663B2 (en) 2015-07-08 2024-05-14 American Gene Technologies International Inc. HIV pre-immunization and immunotherapy
US10137144B2 (en) 2016-01-15 2018-11-27 American Gene Technologies International Inc. Methods and compositions for the activation of gamma-delta T-cells
AU2017207906B2 (en) 2016-01-15 2021-03-11 American Gene Technologies International Inc. Methods and compositions for the activation of gamma-delta T-cells
US10888613B2 (en) 2016-02-08 2021-01-12 American Gene Technologies International Inc. Method of producing cells resistant to HIV infection
EP4036231A1 (en) 2016-03-09 2022-08-03 American Gene Technologies International Inc. Combination vectors and methods for treating cancer
WO2017213697A1 (en) 2016-06-08 2017-12-14 American Gene Technologies International Inc. Non-integrating viral delivery system and methods related thereto
AU2017292582C1 (en) 2016-07-08 2021-11-11 American Gene Technologies International Inc. HIV pre-immunization and immunotherapy
EP3487507A4 (en) 2016-07-21 2020-04-08 American Gene Technologies International, Inc. VIRAL VECTORS FOR TREATING PARKINSON'S DISEASE
EP3607072A4 (en) 2017-04-03 2021-01-06 American Gene Technologies International Inc. Compositions and methods for treating phenylketonuria

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
KAPOOR P ET AL: "Reconstitution of Epstein-Barr virus-based plasmid partitioning in budding yeast", 15 January 2001, EMBO JOURNAL, OXFORD UNIVERSITY PRESS, SURREY, GB, PAGE(S) 222-230, ISSN: 0261-4189, XP002266782 *
LIN J H ET AL: "Expression efficiency of the human thrombomodulin-encoding gene in various vector and host systems", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 147, no. 2, 30 September 1994 (1994-09-30), pages 287 - 292, XP002091310, ISSN: 0378-1119 *
MEISSNER PETRA ET AL: "Transient gene expression: Recombinant protein production with suspension-adapted HEK293-EBNA cells", 20 October 2001, BIOTECHNOLOGY AND BIOENGINEERING, VOL. 75, NR. 2, PAGE(S) 197-203, ISSN: 0006-3592, XP002283991 *
WENDELBURG ET AL: "AN ENHANCED EBNA1 VARIANT WITH REDUCED IR3 DOMAIN FOR LONG-TERM EPISOMAL MAINTENANCE AND TRANSGENE EXPRESSION OF ORIP-BASED PLASMIDS IN HUMAN CELLS", GENE THERAPY, MACMILLAN PRESS LTD., BASINGSTOKE, GB, vol. 5, May 1998 (1998-05-01), pages 1389 - 1399, XP002931315, ISSN: 0969-7128 *
WUTTKE M ET AL: "Structural characterization of human recombinant and bone-derived bone sialoprotein: Functional implications for cell attachment and hydroxyapatite binding", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 276, no. 39, 28 September 2001 (2001-09-28), pages 36839 - 36848, XP002230944, ISSN: 0021-9258 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7371542B2 (en) 2003-07-11 2008-05-13 Cytos Biotechnology Ag Gene expression system

Also Published As

Publication number Publication date
AU2003283174A8 (en) 2004-06-30
WO2004053137A2 (en) 2004-06-24
AU2003283174A1 (en) 2004-06-30

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