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WO2004052103A1 - Procede de preparation d'une formulation de biopesticide a utiliser contre le scloyte des graines de cafe (cbb) - Google Patents

Procede de preparation d'une formulation de biopesticide a utiliser contre le scloyte des graines de cafe (cbb) Download PDF

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Publication number
WO2004052103A1
WO2004052103A1 PCT/IN2003/000355 IN0300355W WO2004052103A1 WO 2004052103 A1 WO2004052103 A1 WO 2004052103A1 IN 0300355 W IN0300355 W IN 0300355W WO 2004052103 A1 WO2004052103 A1 WO 2004052103A1
Authority
WO
WIPO (PCT)
Prior art keywords
mycelial
lit
preparing
bassiana
fungus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IN2003/000355
Other languages
English (en)
Inventor
S. R. Niranjana
Shekar H. Shetty
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Department of Biotechnology of Ministry of Science and Technology India
University of Mysore
Original Assignee
Department of Biotechnology of Ministry of Science and Technology India
University of Mysore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Department of Biotechnology of Ministry of Science and Technology India, University of Mysore filed Critical Department of Biotechnology of Ministry of Science and Technology India
Priority to AU2003276691A priority Critical patent/AU2003276691A1/en
Publication of WO2004052103A1 publication Critical patent/WO2004052103A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom

Definitions

  • This invention relates to the process of preparing a biopestieide formulation for use against coffee berry borer (CBB).
  • This method of control is not only uneconomical but also hazardous to the environment destroying most of the non-target beneficial insect populations in the plantations like Honey bees, Ladybird beetle etc., and other beneficial microorganisms.
  • Coffee berry borer develops resistance to this chemical very fast in 2 to 3 generations. Once the CBB develops resistance, only the non-target insects are killed and CBB thrives more profusely. Endosulfan residue that remains in coffee beans has deleterious effects on humans.
  • Be uveri has been reported to cause infective mortality in many terrestrial and aquatic insect pests (Bidochka and Khachatourians, 1991) and also has been successfully used against European corn borer, migratory grass hopper, green house whitefly, pine caterpillar codling moth, mosquitoes and lygus bug (Bidochkha and Khachatourians, 1991 and Khachatourians, 1992). Larval and adult stages of several insects are hosts to many Deuteromycetouos fungal species. These fungi cause mycosis to both Coleopterans and Lepidopterans. Among these pathogens, Beauveria, Metarhisium and Paecilomyces spp.
  • B. bassiana has been successfully used as foliar applications by several researchers for suppressing populations of several insects, like Bemisia tabaci (Gennadius) (Wright, 1992; Carruthers et at, 1993), Ostrinia nubilalis (Hubner) (Lewis et al., 1996), and Leptinotarsa decemlineata (Say) (Poprawski et at, 1997).
  • B. bassiana has been shown as an important entomopathogen in controlling diamondback moth, Plutella xylostella (L.) by Vandenberg et al. (1998).
  • -5. bassiana has been successfully used for control of P. xylostella with crucifer transplants by Shelton et al. (1998).
  • the effect of ento ogenous fungus B. bassiana was tested on insect predators like Coccinella sp. t C.
  • Doane (1959) reported epizootic among larvae of the small elm bark beetle, Scolytus multistriatus in the United States, caused by -5. bassiana. The report suggested the possibility of employing entomogenous fungi for the biological control of the elm bark beetles, S. scolytus and S. multistriatus, the vectors of Dutch elm disease in Germany.
  • bassiana has been successfully used as foliar applications by several researchers for suppressing populations of several insects, like Bemisia tabaci (Gennadius) (Wright, 1992; Carruthers et at, 1993), Ostrinia nubilalis (Hubner) (Lewis et al., 1996), and Leptinotarsa decemlineata (Say) (Poprawski et al., 1997).
  • Bemisia tabaci Genenadius
  • Hubner Ostrinia nubilalis
  • Say Leptinotarsa decemlineata
  • bassiana production has recently been developed by Mycotech Corporation, USA (Bradley et al., 1992).
  • a corn starch and corn starch oil formulations of B. bassiana mycelia as well as pure dry mycelial preparations was obtained by Feng et al. (1994).
  • Bidochka et al. (1997) have found out that hydrophobic interactions, appressoria formation, and mucus production by the fungus are involved in adhesion to the cuticle.
  • the technology of protoplast fusion is a valuable method for intra- and inter specific hybridization. Through this a somatic hybrid of a cross between two strains of B. bassiana has been obtained (Viaud, 1998).
  • An object of this invention is to propose a process for preparing a biopesticide formulation against coffee berry borer.
  • object of this invention is to propose a formulation containing entomopathogenic fungus which is eco-friendly.
  • Yet another object of this invention is to prepare a formulation which is economical and easy to deliver.
  • CBB coffee berry borer
  • a process for preparing talcum powder formulation comprising; harvesting mycelial mat of B. bassiana from roux bottles; sterilizing purified talcum powder; mixing sterile talcum powder with the harvested mycelial mat; mixing sterilized carboxy m thyl cellulose; spreading out the fo ⁇ nulation in trays for drying.
  • the fungus is first isolated from coffee berry borer (CBB) cadavers.
  • CBB cadavers were washed with sterile distilled water, homogenized using a pestle and mortar in sterile distilled water, serially diluted and plated on
  • PDA Potato Dextrose Agar
  • the fungus was cultured on Dextrose-Yeast extract liquid medium supplemented with peptone (YPD). The fungus formed a white mycelial mat and sporulated after 12-14 days of incubation at 25 ⁇ 2 0 C.
  • YPD peptone
  • a mycelial agar plug cut out from the actively growing seed culture on specific medium (YPD A) was inoculated into roux culture bottles containing sterile YPD broth.
  • the broth was decanted without mixing.
  • the mycelial mat was taken into a clean container and stored at 4°C for use with in 24 hours.
  • To prepare lyophilized culture after the mycelial mat is taken from the Roux bottle it is put into round bottom corning flask frozen at -40°C for 6 hours and connected to a manifold assembly for lyophilisation. After lyophilisation the dry mycelia mat is stored under-40°C for use within 60 days.
  • Formulation 1 Fresh culture in liquid broth Fresh culture is produced in specific YPD liquid medium in Roux bottles.
  • YPD broth is prepared and 100 ml is dispensed into Roux bottles.
  • Formulation of B. bassiana was prepared using purified talcum powder.
  • Mycelial mat was harvested from roux bottles blot dried and mixed with

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé de préparation d'une formulation de biopesticide consistant à isoler le champignon d'un cadavre de scloyte des graines de café (CBB) ; à préparer un milieu liquide ; à cultiver ledit champignon d'isolement de B. bassiana dans le support liquide afin de former une trame mycélienne ; à inoculer des disques d'agar mycéliens découpés dans la trame mycélienne à croissance active pour les mettre dans un milieu de bouillon ; à incuber le bouillon ; à récolter les conidies mycéliennes par décantation.
PCT/IN2003/000355 2002-12-12 2003-11-05 Procede de preparation d'une formulation de biopesticide a utiliser contre le scloyte des graines de cafe (cbb) Ceased WO2004052103A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003276691A AU2003276691A1 (en) 2002-12-12 2003-11-05 The process of preparing a biopesticide formulation for use against coffee berry borer (cbb)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN1244/DEL/2002 2002-12-12
IN1244DE2002 2002-12-12

Publications (1)

Publication Number Publication Date
WO2004052103A1 true WO2004052103A1 (fr) 2004-06-24

Family

ID=32500464

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2003/000355 Ceased WO2004052103A1 (fr) 2002-12-12 2003-11-05 Procede de preparation d'une formulation de biopesticide a utiliser contre le scloyte des graines de cafe (cbb)

Country Status (4)

Country Link
AU (1) AU2003276691A1 (fr)
TR (1) TR200503421T2 (fr)
WO (1) WO2004052103A1 (fr)
ZA (1) ZA200505006B (fr)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8623420B2 (en) 2006-12-08 2014-01-07 University Of Vermont And State Agriculture College Sweet whey based biopesticide composition
US9578873B2 (en) 2011-10-11 2017-02-28 Universidad Nacional De Quilmes Methods for controlling leaf-cutting ants
WO2017151684A1 (fr) * 2016-03-01 2017-09-08 Sustainable Bioproducts Holdings, Llc Biomats fongiques filamenteux, procédés de production et procédés d'utilisation correspondants
US10851396B2 (en) 2014-07-03 2020-12-01 The Fynder Group, Inc. Acidophilic fusarium oxysporum strains, methods of their production and methods of their use
KR20210063760A (ko) * 2019-11-25 2021-06-02 영남대학교 산학협력단 진균을 이용한 스캐폴드 제조 및 이를 이용한 조직 재생방법
US11039635B2 (en) 2019-02-27 2021-06-22 The Fynder Group, Inc. Food materials comprising filamentous fungal particles
US11118305B2 (en) 2019-06-18 2021-09-14 The Fynder Group, Inc. Fungal textile materials and leather analogs
CN114071992A (zh) * 2020-02-03 2022-02-18 麦克沃克斯股份有限公司 菌丝体以及基于菌丝体的材料的新型微结构
US11297866B2 (en) 2017-08-30 2022-04-12 The Fynder Group, Inc. Bioreactor system for the cultivation of filamentous fungal biomass

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU810180A1 (ru) * 1979-08-06 1981-03-07 Научно-Исследовательская Лабораториякормовых Ресурсов Леса Всесоюзнойакдемии Сельскохозяйственных Науким.B.И.Ленина Питательна среда дл выращивани
WO1992011856A1 (fr) * 1991-01-09 1992-07-23 Wright James E Composition biopesticide et procede d'elimination d'insectes nuisibles

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU810180A1 (ru) * 1979-08-06 1981-03-07 Научно-Исследовательская Лабораториякормовых Ресурсов Леса Всесоюзнойакдемии Сельскохозяйственных Науким.B.И.Ленина Питательна среда дл выращивани
WO1992011856A1 (fr) * 1991-01-09 1992-07-23 Wright James E Composition biopesticide et procede d'elimination d'insectes nuisibles

Cited By (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8623420B2 (en) 2006-12-08 2014-01-07 University Of Vermont And State Agriculture College Sweet whey based biopesticide composition
US9578873B2 (en) 2011-10-11 2017-02-28 Universidad Nacional De Quilmes Methods for controlling leaf-cutting ants
US10851396B2 (en) 2014-07-03 2020-12-01 The Fynder Group, Inc. Acidophilic fusarium oxysporum strains, methods of their production and methods of their use
EP3423561B1 (fr) 2016-03-01 2021-04-21 The Fynder Group, Inc. Nattes de biomasse fongique filamenteuse, méthodes pour les produire et methodes de les utiliser
US11001801B2 (en) 2016-03-01 2021-05-11 The Fynder Group, Inc. Filamentous fungal biomats, methods of their production and methods of their use
US10577579B2 (en) 2016-03-01 2020-03-03 Sustainable Bioproducts, Inc. Filamentous fungal biomats, methods of their production and methods of their use
US10590379B2 (en) 2016-03-01 2020-03-17 Sustainable Bioproducts, Inc. Filamentous fungal biomats, methods of their production and methods of their use
CN111019836A (zh) * 2016-03-01 2020-04-17 可持续生物制品公司 丝状真菌生物垫、及其生产方法和使用方法
US10787638B2 (en) 2016-03-01 2020-09-29 The Fynder Group, Inc. Filamentous fungal biomats, methods of their production and methods of their use
CN109153964A (zh) * 2016-03-01 2019-01-04 可持续生物制品公司 丝状真菌生物垫、及其生产方法和使用方法
WO2017151684A1 (fr) * 2016-03-01 2017-09-08 Sustainable Bioproducts Holdings, Llc Biomats fongiques filamenteux, procédés de production et procédés d'utilisation correspondants
CN111019836B (zh) * 2016-03-01 2021-05-04 芬德集团公司 丝状真菌生物垫、及其生产方法和使用方法
US10533155B2 (en) 2016-03-01 2020-01-14 Sustainable Bioproducts, Inc. Filamentous fungal biomats, methods of their production and methods of their use
US11015168B2 (en) 2016-03-01 2021-05-25 The Fynder Group, Inc. Filamentous fungal biomats, methods of their production and methods of their use
US12157880B2 (en) 2016-03-01 2024-12-03 The Fynder Group, Inc. Filamentous fungal biomats, methods of their production and methods of their use
EP3423561B2 (fr) † 2016-03-01 2024-02-28 The Fynder Group, Inc. Nattes de biomasse fongique filamenteuse, méthodes pour les produire et methodes de les utiliser
AU2021201888B2 (en) * 2016-03-01 2023-11-09 The Fynder Group, Inc. Filamentous fungal biomats, methods of their production and methods of their use
EP3901246A1 (fr) * 2016-03-01 2021-10-27 The Fynder Group, Inc. Biomats fongiques filamenteux, leurs procédés de production et leurs procédés d'utilisation
CN109153964B (zh) * 2016-03-01 2023-06-13 芬德集团公司 丝状真菌生物垫、及其生产方法和使用方法
US11505779B2 (en) 2016-03-01 2022-11-22 The Fynder Group, Inc. Filamentous fungal biomats, methods of their production and methods of their use
US11261420B2 (en) 2016-03-01 2022-03-01 The Fynder Group, Inc. Filamentous fungal biomats, methods of their production and methods of their use
US11464251B2 (en) 2017-08-30 2022-10-11 The Fynder Group, Inc. Edible foodstuffs and bio reactor design
US11297866B2 (en) 2017-08-30 2022-04-12 The Fynder Group, Inc. Bioreactor system for the cultivation of filamentous fungal biomass
US11478007B2 (en) 2019-02-27 2022-10-25 The Fynder Group, Inc. Food materials comprising filamentous fungal particles and membrane bioreactor design
US11039635B2 (en) 2019-02-27 2021-06-22 The Fynder Group, Inc. Food materials comprising filamentous fungal particles
US11432575B2 (en) 2019-02-27 2022-09-06 The Fynder Group, Inc. Food materials comprising filamentous fungal particles and membrane bioreactor design
US12415983B2 (en) 2019-02-27 2025-09-16 The Fynder Group, Inc. Food materials comprising filamentous fungal particles and membrane bioreactor design
US11272726B2 (en) 2019-02-27 2022-03-15 The Fynder Group, Inc. Food materials comprising filamentous fungal particles and membrane bioreactor design
US11649586B2 (en) 2019-06-18 2023-05-16 The Fynder Group, Inc. Fungal textile materials and leather analogs
US11427957B2 (en) 2019-06-18 2022-08-30 The Fynder Group, Inc. Fungal textile materials and leather analogs
US11718954B2 (en) 2019-06-18 2023-08-08 The Fynder Group, Inc. Fungal textile materials and leather analogs
US11118305B2 (en) 2019-06-18 2021-09-14 The Fynder Group, Inc. Fungal textile materials and leather analogs
US11414815B2 (en) 2019-06-18 2022-08-16 The Fynder Group, Inc. Fungal textile materials and leather analogs
US11447913B2 (en) 2019-06-18 2022-09-20 The Fynder Group, Inc. Fungal textile materials and leather analogs
KR102334114B1 (ko) 2019-11-25 2021-12-02 영남대학교 산학협력단 진균을 이용한 스캐폴드 제조 및 이를 이용한 조직 재생방법
KR20210063760A (ko) * 2019-11-25 2021-06-02 영남대학교 산학협력단 진균을 이용한 스캐폴드 제조 및 이를 이용한 조직 재생방법
CN114071992A (zh) * 2020-02-03 2022-02-18 麦克沃克斯股份有限公司 菌丝体以及基于菌丝体的材料的新型微结构

Also Published As

Publication number Publication date
AU2003276691A1 (en) 2004-06-30
AU2003276691A8 (en) 2004-06-30
TR200503421T2 (tr) 2008-07-21
ZA200505006B (fr) 2006-07-26

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