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WO2004046731A3 - Method for analysing amino acids, peptides and proteins using mass spectroscopy of fixed charge-modified derivatives - Google Patents

Method for analysing amino acids, peptides and proteins using mass spectroscopy of fixed charge-modified derivatives Download PDF

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Publication number
WO2004046731A3
WO2004046731A3 PCT/US2003/036739 US0336739W WO2004046731A3 WO 2004046731 A3 WO2004046731 A3 WO 2004046731A3 US 0336739 W US0336739 W US 0336739W WO 2004046731 A3 WO2004046731 A3 WO 2004046731A3
Authority
WO
WIPO (PCT)
Prior art keywords
mass
charge
amino acids
protein
fixed charge
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/036739
Other languages
French (fr)
Other versions
WO2004046731A2 (en
Inventor
Gavin Reid
Richard Simpson
Richard O'hair
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ludwig Institute for Cancer Research Ltd
Ludwig Cancer Research
Original Assignee
Ludwig Institute for Cancer Research Ltd
Ludwig Cancer Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ludwig Institute for Cancer Research Ltd, Ludwig Cancer Research filed Critical Ludwig Institute for Cancer Research Ltd
Priority to JP2004553836A priority Critical patent/JP2006506647A/en
Priority to EP03781974A priority patent/EP1565752A2/en
Priority to US10/535,389 priority patent/US20060094121A1/en
Priority to AU2003287673A priority patent/AU2003287673A1/en
Publication of WO2004046731A2 publication Critical patent/WO2004046731A2/en
Anticipated expiration legal-status Critical
Publication of WO2004046731A3 publication Critical patent/WO2004046731A3/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

The invention provides methods, reagents and kits for amino acid, peptide and protein identification, differential quantitation and for the analysis of post translational modification and cross-linking status, comprising: derivatizing a mixture of amino acids peptides or proteins, to form at least one amino acid peptide or protein containing a fixed-charge ion, other than at the C-terminal or N-terminal end thereof; introducing the mixture of amino acids peptides or proteins containing the fixed charge derivatized amino acid peptide or protein to a mass spectrometer; passing the mixture of amino acids peptides or proteins containing the fixed charge derivatized amino acid peptide or protein through a first mass resolving spectrometer to select precursor protein or peptide ions having a first desired mass-to-charge ratio; subjecting the precursor ions of the first mass to charge ratio to dissociation to form a product ion having a second mass-to-charge ratio that is characteristic of a fragmentation occurring at a site adjacent to the fixed charge; and detecting the product ions having the second mass-to-charge ratio. The product ion having the second mass-to-charge ratio may be either a product ion formed by neutral loss of the fixed charge from the precursor ion, or a product ion formed by charged loss of the fixed charge from the precursor ion.
PCT/US2003/036739 2002-11-18 2003-11-18 Method for analysing amino acids, peptides and proteins using mass spectroscopy of fixed charge-modified derivatives Ceased WO2004046731A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2004553836A JP2006506647A (en) 2002-11-18 2003-11-18 Method for analyzing amino acids, peptides and proteins
EP03781974A EP1565752A2 (en) 2002-11-18 2003-11-18 Method for analysing amino acids, peptides and proteins using mass spectroscopy of fixed charge-modified derivates
US10/535,389 US20060094121A1 (en) 2002-11-18 2003-11-18 Method for analysing amino acids, peptides and proteins
AU2003287673A AU2003287673A1 (en) 2002-11-18 2003-11-18 Method for analysing amino acids, peptides and proteins using mass spectroscopy of fixed charge-modified derivatives

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2002952747A AU2002952747A0 (en) 2002-11-18 2002-11-18 Method for analysing peptides
AU2002952747 2002-11-28

Publications (2)

Publication Number Publication Date
WO2004046731A2 WO2004046731A2 (en) 2004-06-03
WO2004046731A3 true WO2004046731A3 (en) 2005-06-23

Family

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Family Applications (1)

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PCT/US2003/036739 Ceased WO2004046731A2 (en) 2002-11-18 2003-11-18 Method for analysing amino acids, peptides and proteins using mass spectroscopy of fixed charge-modified derivatives

Country Status (4)

Country Link
EP (1) EP1565752A2 (en)
JP (1) JP2006506647A (en)
AU (1) AU2002952747A0 (en)
WO (1) WO2004046731A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9196466B2 (en) 2002-07-24 2015-11-24 Micromass Uk Limited Mass spectrometer with bypass of a fragmentation device

Families Citing this family (19)

* Cited by examiner, † Cited by third party
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US20020115056A1 (en) 2000-12-26 2002-08-22 Goodlett David R. Rapid and quantitative proteome analysis and related methods
SE0401293D0 (en) * 2004-05-17 2004-05-17 Amersham Biosciences Ab Novel MS Reagents
KR100611313B1 (en) * 2004-06-30 2006-08-10 고려대학교 산학협력단 N-terminal substitution of polypeptide, amino acid sequencing and quantification method in polypeptide
WO2006023398A2 (en) * 2004-08-16 2006-03-02 Ludwig Institute For Cancer Research Modular isotope labelled mass spectrometry reagents and methods for quantitation of amino acids, peptides and proteins
US20090053817A1 (en) * 2004-08-16 2009-02-26 Ludwig Institute For Cancer Research Fixed charge reagents
US20060183238A1 (en) * 2005-02-09 2006-08-17 Applera Corporation Amine-containing compound analysis methods
GB0523806D0 (en) * 2005-11-23 2006-01-04 Micromass Ltd Mass spectrometer
GB0523811D0 (en) * 2005-11-23 2006-01-04 Micromass Ltd Mass stectrometer
WO2008111911A1 (en) * 2007-03-13 2008-09-18 Nanyang Polytechnic System and process for pre-filtering of tandem mass spectra using piecewise convolution
DE602008001609D1 (en) 2007-03-19 2010-08-05 Brains Online Holding B V METHOD FOR DETERMINING AN ANALYTE WITH A PRIMARY AMINO GROUP AND KIT FOR LABELING THIS ANALYSIS
US9202678B2 (en) 2008-11-14 2015-12-01 Board Of Trustees Of Michigan State University Ultrafast laser system for biological mass spectrometry
US8372653B2 (en) * 2010-01-22 2013-02-12 Dh Technologies Development Pte. Ltd. Mass tag reagents for simultaneous quantitation and identification of small molecules
JP5799618B2 (en) * 2011-07-06 2015-10-28 株式会社島津製作所 MS / MS mass spectrometer and program for the same
US9581605B2 (en) 2012-05-22 2017-02-28 Biogen Ma Inc. Trapping reagents for reactive metabolites screening
CN105051530B (en) 2013-03-14 2018-05-01 莱克公司 System and method for Tandem Mass Spectrometry Analysis
EP3023420A1 (en) 2014-11-18 2016-05-25 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Methods for providing stable isoindole derivatives
CN110133124A (en) * 2019-04-29 2019-08-16 天津中医药大学 The content assaying method of 18 kinds of amino acid in SHUXUETONG ZHUSHEYE
CN115515928B (en) * 2020-02-28 2024-05-10 公立大学法人大阪 Method for analyzing polysulfide, method for inhibiting hydrolysis of polysulfide, and use of N-iodoacetyl-L-tyrosine methyl ester
CN112786105B (en) * 2020-12-07 2024-05-07 中山大学附属第五医院 A metaproteome mining method and its application in obtaining intestinal microbial proteolytic characteristics

Non-Patent Citations (11)

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Title
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BRANCIA F L ET AL: "IMPROVED MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRIC ANALYSIS OF TRYPTIC HYDROLYSATES OF PROTEINS FOLLOWING GUANIDINATION OF LYSINE-CONTAINING PEPTIDES", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, HEYDEN, LONDON, GB, vol. 14, no. 21, 2000, pages 2070 - 2073, XP009018972, ISSN: 0951-4198 *
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9196466B2 (en) 2002-07-24 2015-11-24 Micromass Uk Limited Mass spectrometer with bypass of a fragmentation device

Also Published As

Publication number Publication date
EP1565752A2 (en) 2005-08-24
JP2006506647A (en) 2006-02-23
WO2004046731A2 (en) 2004-06-03
AU2002952747A0 (en) 2002-12-05

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