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WO2004046100A3 - Release of the self-quenching of fluorescence near silver metallic surfaces - Google Patents

Release of the self-quenching of fluorescence near silver metallic surfaces Download PDF

Info

Publication number
WO2004046100A3
WO2004046100A3 PCT/US2003/035049 US0335049W WO2004046100A3 WO 2004046100 A3 WO2004046100 A3 WO 2004046100A3 US 0335049 W US0335049 W US 0335049W WO 2004046100 A3 WO2004046100 A3 WO 2004046100A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorophore
labeled
self
biomolecule
metallic particle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/035049
Other languages
French (fr)
Other versions
WO2004046100A2 (en
Inventor
Joseph R Lakowicz
Joanna B Malicka
Ignacy Gryczynski
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Maryland Baltimore
University of Maryland College Park
Original Assignee
University of Maryland Baltimore
University of Maryland College Park
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Maryland Baltimore, University of Maryland College Park filed Critical University of Maryland Baltimore
Priority to AU2003291718A priority Critical patent/AU2003291718A1/en
Publication of WO2004046100A2 publication Critical patent/WO2004046100A2/en
Publication of WO2004046100A3 publication Critical patent/WO2004046100A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Inorganic Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Materials, systems and methods for reducing self-quenching due to homo resonance energy transfer when biomolecules are labeled with more than one fluorophore. The materials include a fluorophore labeled biomolecule conjugate and a metallic particle, wherein the labeled biomolecule conjugate comprises multiple fluorophores per biomolecule. The fluorophore-biomolecule conjugate may be bound to the metallic particle or positioned at a distance from the metallic particle, wherein the position of the fluorophore labeled biomolecule conjugate to the metallic particle reduces self-quenching due to homo resonance energy transfer. The methods include detecting a presence of or quantifying an amount of a biomolecule. The fluorophore labeled biomolecules and metallic particles can be used to obtain ultra-bright reagents for use in immunoassays, imaging, arrays and other applications.
PCT/US2003/035049 2002-11-15 2003-11-17 Release of the self-quenching of fluorescence near silver metallic surfaces Ceased WO2004046100A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003291718A AU2003291718A1 (en) 2002-11-15 2003-11-17 Release of the self-quenching of fluorescence near silver metallic surfaces

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42677802P 2002-11-15 2002-11-15
US60/426,778 2002-11-15

Publications (2)

Publication Number Publication Date
WO2004046100A2 WO2004046100A2 (en) 2004-06-03
WO2004046100A3 true WO2004046100A3 (en) 2004-09-30

Family

ID=32326423

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/035049 Ceased WO2004046100A2 (en) 2002-11-15 2003-11-17 Release of the self-quenching of fluorescence near silver metallic surfaces

Country Status (2)

Country Link
AU (1) AU2003291718A1 (en)
WO (1) WO2004046100A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7871573B2 (en) * 2004-07-26 2011-01-18 University Of Louisville Research Foundation, Inc. Enhancement of sensitivity of fluorophore mediated biosensing and bioimaging
US8563328B2 (en) 2004-07-26 2013-10-22 University Of Louisville Research Foundation, Inc. Fiber-optic biosensor and biosensing methods
GB2431233A (en) * 2005-10-14 2007-04-18 E2V Tech Molecular detector arrangement
US9140707B2 (en) 2007-08-10 2015-09-22 University Of Louisville Research Foundation, Inc. Sensors and methods for detecting diseases caused by a single point mutation

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5501985A (en) * 1990-07-18 1996-03-26 Abbott Laboratories Analyte-substitute reagent for use in specific binding assay methods, devices and kits
US6284465B1 (en) * 1999-04-15 2001-09-04 Agilent Technologies, Inc. Apparatus, systems and method for locating nucleic acids bound to surfaces
US6312906B1 (en) * 1999-01-15 2001-11-06 Imperial College Innovations, Ltd. Immobilized nucleic acid hybridization reagent and method
US6327410B1 (en) * 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US6369206B1 (en) * 1994-07-29 2002-04-09 Robert D. Leone Metal organothiol particles
US6586193B2 (en) * 1996-04-25 2003-07-01 Genicon Sciences Corporation Analyte assay using particulate labels

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5501985A (en) * 1990-07-18 1996-03-26 Abbott Laboratories Analyte-substitute reagent for use in specific binding assay methods, devices and kits
US6369206B1 (en) * 1994-07-29 2002-04-09 Robert D. Leone Metal organothiol particles
US6586193B2 (en) * 1996-04-25 2003-07-01 Genicon Sciences Corporation Analyte assay using particulate labels
US6327410B1 (en) * 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US6312906B1 (en) * 1999-01-15 2001-11-06 Imperial College Innovations, Ltd. Immobilized nucleic acid hybridization reagent and method
US6284465B1 (en) * 1999-04-15 2001-09-04 Agilent Technologies, Inc. Apparatus, systems and method for locating nucleic acids bound to surfaces

Also Published As

Publication number Publication date
AU2003291718A1 (en) 2004-06-15
AU2003291718A8 (en) 2004-06-15
WO2004046100A2 (en) 2004-06-03

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