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WO2003104424A3 - NOVEL MOLECULAR CONJUGATES USED FOR SIGNAL AMPLIFICATION - Google Patents

NOVEL MOLECULAR CONJUGATES USED FOR SIGNAL AMPLIFICATION Download PDF

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Publication number
WO2003104424A3
WO2003104424A3 PCT/US2003/018185 US0318185W WO03104424A3 WO 2003104424 A3 WO2003104424 A3 WO 2003104424A3 US 0318185 W US0318185 W US 0318185W WO 03104424 A3 WO03104424 A3 WO 03104424A3
Authority
WO
WIPO (PCT)
Prior art keywords
signal amplification
molecular conjugates
novel molecular
conjugates used
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/018185
Other languages
French (fr)
Other versions
WO2003104424A2 (en
Inventor
Subhash Dhawan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Health and Human Services
Original Assignee
US Department of Health and Human Services
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by US Department of Health and Human Services filed Critical US Department of Health and Human Services
Priority to AU2003251428A priority Critical patent/AU2003251428A1/en
Publication of WO2003104424A2 publication Critical patent/WO2003104424A2/en
Anticipated expiration legal-status Critical
Publication of WO2003104424A3 publication Critical patent/WO2003104424A3/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • AIDS & HIV (AREA)
  • Food Science & Technology (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne des conjugués moléculaires qui assurent une amplification améliorée du signal dans des études analytiques comprenant l'utilisation de réactifs de liaison spécifiques à l'analyte qui sont associés à un marqueur détectable. Cette invention concerne également des trousses et des méthodes d'utilisation des conjugués moléculaires selon la présente invention.The present invention relates to molecular conjugates which provide improved signal amplification in analytical studies including the use of analyte specific binding reagents which are associated with a detectable label. This invention also relates to kits and methods of using the molecular conjugates of the present invention.

PCT/US2003/018185 2002-06-10 2003-06-09 Novel molecular conjugates for signal amplification Ceased WO2003104424A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003251428A AU2003251428A1 (en) 2002-06-10 2003-06-09 Novel molecular conjugates for signal amplification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38811502P 2002-06-10 2002-06-10
US60/388,115 2002-06-10

Publications (2)

Publication Number Publication Date
WO2003104424A2 WO2003104424A2 (en) 2003-12-18
WO2003104424A3 true WO2003104424A3 (en) 2005-04-28

Family

ID=29736425

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/018185 Ceased WO2003104424A2 (en) 2002-06-10 2003-06-09 Novel molecular conjugates for signal amplification

Country Status (2)

Country Link
AU (1) AU2003251428A1 (en)
WO (1) WO2003104424A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040018495A1 (en) * 2002-07-24 2004-01-29 Xing-Xiang Li Microparticle based signal amplification for the detection of analytes
WO2009055387A1 (en) 2007-10-22 2009-04-30 Thermo Fisher Scientific Polymerized conjugates for biological applications
GB2470939A (en) * 2009-06-10 2010-12-15 Dna Supernova Ltd Signal amplification microspheres
US9200033B2 (en) 2011-05-24 2015-12-01 Karebay Biochem Inc. Enzyme-degradable polymer and application thereof
CN103513026B (en) * 2011-12-30 2015-04-29 吴坚 Signal amplification type immunofluorescence probe, and preparation method and application thereof
ES2636483T3 (en) * 2012-02-21 2017-10-05 Laboratory Corporation Of America Holdings Methods and systems for bioassay signal amplification
RU2744836C2 (en) * 2014-05-08 2021-03-16 Новодиакс, Инк. Direct immunohistochemical analysis
WO2017100442A1 (en) * 2015-12-08 2017-06-15 SeLux Diagnostics, Inc. Nanoparticle amplifiers and uses thereof
US10962530B2 (en) 2016-03-21 2021-03-30 National Cheng Kung University Molecular probe for signal amplification and kit and assay using the same
EP3379252B1 (en) * 2017-03-20 2022-10-26 National Cheng Kung University Molecular probe for signal amplification and assay using the same
CN113311174B (en) * 2021-05-14 2022-05-13 宁波瑞源生物科技有限公司 Myoglobin antibody-enzyme marker and preparation and application thereof
CN115586335B (en) * 2021-11-04 2023-10-13 贵州美鑫达医疗科技有限公司 Polymerase-antibody combination for rapid immunohistochemistry in melanoma surgery

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020150919A1 (en) * 2000-10-27 2002-10-17 Sherman Weismann Methods for identifying genes associated with diseases or specific phenotypes
US20020155451A1 (en) * 1998-12-30 2002-10-24 Dana-Farber Cancer Institute, Inc. Mutation scanning array, and methods of use thereof
US20030134333A1 (en) * 2000-04-28 2003-07-17 Peter Dehlinger Tagged microparticle compositions and methods

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020155451A1 (en) * 1998-12-30 2002-10-24 Dana-Farber Cancer Institute, Inc. Mutation scanning array, and methods of use thereof
US20030134333A1 (en) * 2000-04-28 2003-07-17 Peter Dehlinger Tagged microparticle compositions and methods
US20020150919A1 (en) * 2000-10-27 2002-10-17 Sherman Weismann Methods for identifying genes associated with diseases or specific phenotypes

Also Published As

Publication number Publication date
AU2003251428A8 (en) 2003-12-22
WO2003104424A2 (en) 2003-12-18
AU2003251428A1 (en) 2003-12-22

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