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WO2003104476A3 - DETECTION OF REPLICATION-SUITABLE VIRUSES - Google Patents

DETECTION OF REPLICATION-SUITABLE VIRUSES Download PDF

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Publication number
WO2003104476A3
WO2003104476A3 PCT/US2003/018243 US0318243W WO03104476A3 WO 2003104476 A3 WO2003104476 A3 WO 2003104476A3 US 0318243 W US0318243 W US 0318243W WO 03104476 A3 WO03104476 A3 WO 03104476A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
virus
viral
preparation
analysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/018243
Other languages
French (fr)
Other versions
WO2003104476A2 (en
Inventor
Mark Bowe
Russette M Lyons
Tracey Walker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Novartis AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis AG filed Critical Novartis AG
Priority to CA002488778A priority Critical patent/CA2488778A1/en
Priority to EP03757465A priority patent/EP1513956A2/en
Priority to AU2003261076A priority patent/AU2003261076A1/en
Publication of WO2003104476A2 publication Critical patent/WO2003104476A2/en
Publication of WO2003104476A3 publication Critical patent/WO2003104476A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/13—Tumour cells, irrespective of tissue of origin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention concerne une analyse biologique permettant de détecter un virus apte à la réplication non sélective dans une préparation de virus à réplication sélective (RCV). Le procédé consiste à faire passer la préparation virale au moins une fois sur des cellules qui amplifient de manière différentielle le virus à réplication sélective par comparaison avec un virus apte à la réplication, puis à poursuivre l'analyse de la préparation virale pour y détecter la présence d'un virus apte à la réplication. La poursuite de l'analyse virale comprend une ou plusieurs des opérations suivantes : détection d'un effet cytophathique accru, détection d'une production virale, détection d'un pouvoir de destruction accru de cellules normales, détection d'un schéma de digestion de restriction altéré, détection d'une séquence génomique altérée, détection de recombinants attendus avec amplification de la réaction en chaîne de la polymérase et détection d'une hépato-toxicité aiguë chez un animal auquel on a administré la préparation virale. Dans un mode de réalisation, l'analyse permet de détecter des adénovirus aptes à la réplication dans une préparation d'adénovirus oncolytique.This invention relates to a biological analysis for detecting a virus capable of non-selective replication in a preparation of selective replication virus (RCV). The method comprises passing the viral preparation at least once over cells which differentially amplify the selectively replicating virus by comparison with a replicable virus, then continuing the analysis of the viral preparation to detect therein. presence of a replicable virus. The continuation of the viral analysis includes one or more of the following operations: detection of an increased cytophathic effect, detection of a viral production, detection of an increased destructive power of normal cells, detection of a pattern of digestion of altered restriction, detection of an altered genomic sequence, detection of expected recombinants with amplification of the polymerase chain reaction and detection of acute hepatotoxicity in an animal to which the viral preparation has been administered. In one embodiment, the analysis makes it possible to detect adenoviruses capable of replication in an oncolytic adenovirus preparation.

PCT/US2003/018243 2002-06-07 2003-06-09 Assay to detect replication competent viruses Ceased WO2003104476A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002488778A CA2488778A1 (en) 2002-06-07 2003-06-09 Assay to detect replication competent viruses
EP03757465A EP1513956A2 (en) 2002-06-07 2003-06-09 Assay to detect replication competent viruses
AU2003261076A AU2003261076A1 (en) 2002-06-07 2003-06-09 Assay to detect replication competent viruses

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US38725102P 2002-06-07 2002-06-07
US60/387,251 2002-06-07
US45937503P 2003-03-31 2003-03-31
US60/459,375 2003-03-31

Publications (2)

Publication Number Publication Date
WO2003104476A2 WO2003104476A2 (en) 2003-12-18
WO2003104476A3 true WO2003104476A3 (en) 2004-03-04

Family

ID=29739937

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/018243 Ceased WO2003104476A2 (en) 2002-06-07 2003-06-09 Assay to detect replication competent viruses

Country Status (4)

Country Link
EP (1) EP1513956A2 (en)
AU (1) AU2003261076A1 (en)
CA (1) CA2488778A1 (en)
WO (1) WO2003104476A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6900049B2 (en) 1998-09-10 2005-05-31 Cell Genesys, Inc. Adenovirus vectors containing cell status-specific response elements and methods of use thereof
AU2003265873A1 (en) * 2003-08-28 2005-04-14 Cell Genesys, Inc. Oncolytic adenoviral vectors encoding gm-csf
US7482156B2 (en) 2003-10-15 2009-01-27 Cell Genesys, Inc. Hepatocellular carcinoma specific promoter and uses thereof
US12152281B2 (en) * 2019-05-30 2024-11-26 Trizell Ltd. Gene therapy vector contamination assay
CN115583994B (en) * 2022-11-04 2024-09-24 四川农业大学 A polyclonal antibody for kiwifruit AcVB virus and its preparation method and application

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6248514B1 (en) * 1996-07-09 2001-06-19 Canji, Inc. Methods for measuring viral infectivity
US6265212B1 (en) * 1995-06-15 2001-07-24 Introgene B.V. Packaging systems for human recombinant adenovirus to be used in gene therapy

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6265212B1 (en) * 1995-06-15 2001-07-24 Introgene B.V. Packaging systems for human recombinant adenovirus to be used in gene therapy
US6248514B1 (en) * 1996-07-09 2001-06-19 Canji, Inc. Methods for measuring viral infectivity

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DION ET AL.: "Supernatant rescue assay vs. polymerase chain reaction for detection of wild type adenovirus-contaminating recombinant adenovirus stocks", JOURNAL OF VIROLOGICAL METHODS, vol. 56, 1996, pages 99 - 107, XP002972682 *
LOCHMULLER ET AL.: "Emergence of early region 1-containing replication-competent adenovirus in stocks of replication-defective adenovirus recombinants (detaE1 + deltaE3) during multiple passages in 293 cells", HUMAN GENE THERAPY, vol. 5, December 1994 (1994-12-01), pages 1485 - 1491, XP002972681 *

Also Published As

Publication number Publication date
WO2003104476A2 (en) 2003-12-18
AU2003261076A8 (en) 2003-12-22
AU2003261076A1 (en) 2003-12-22
EP1513956A2 (en) 2005-03-16
CA2488778A1 (en) 2003-12-18

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