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WO2003031947A3 - Gerät zur sequenzierung von nukleinsäuremolekülen - Google Patents

Gerät zur sequenzierung von nukleinsäuremolekülen Download PDF

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Publication number
WO2003031947A3
WO2003031947A3 PCT/EP2002/011098 EP0211098W WO03031947A3 WO 2003031947 A3 WO2003031947 A3 WO 2003031947A3 EP 0211098 W EP0211098 W EP 0211098W WO 03031947 A3 WO03031947 A3 WO 03031947A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecules
incorporated
sequencing nucleic
strands
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2002/011098
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English (en)
French (fr)
Other versions
WO2003031947A2 (de
Inventor
Dmitri Tcherkassov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genovoxx GmbH
Original Assignee
Genovoxx GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genovoxx GmbH filed Critical Genovoxx GmbH
Priority to US10/491,557 priority Critical patent/US20050227231A1/en
Priority to AU2002350485A priority patent/AU2002350485A1/en
Publication of WO2003031947A2 publication Critical patent/WO2003031947A2/de
Publication of WO2003031947A3 publication Critical patent/WO2003031947A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Gegenstand der Erfindung ist ein Gerät zur automatischen Ermittlung von Nukleinsäuresequenzen. Die sequenzierungsreaktion erfolgt durch den parallelen sequentiellen Aufbau der zu einzelnen fixierten einzelsträngigen Nukleinsäureketten komplementären Stränge. Der Sequenzierautomat führt diesen sequentiellen Aufbau durch und detektiert elektromagnetische Strahlung von einzelnen, markierten in die komplementäre Stränge eingebauten Nukleotiden (NT*s). Aus der Reihenfolge der eingebauten NT*s wird die Sequenz der immobilisierten Nukleinsäureketten bestimmt.
PCT/EP2002/011098 2001-10-04 2002-10-02 Gerät zur sequenzierung von nukleinsäuremolekülen Ceased WO2003031947A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/491,557 US20050227231A1 (en) 2001-10-04 2002-10-02 Device for sequencing nucleic acid molecules
AU2002350485A AU2002350485A1 (en) 2001-10-04 2002-10-02 Device for sequencing nucleic acid molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10148868.8 2001-10-04
DE10148868 2001-10-04

Publications (2)

Publication Number Publication Date
WO2003031947A2 WO2003031947A2 (de) 2003-04-17
WO2003031947A3 true WO2003031947A3 (de) 2003-12-04

Family

ID=7701313

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/011098 Ceased WO2003031947A2 (de) 2001-10-04 2002-10-02 Gerät zur sequenzierung von nukleinsäuremolekülen

Country Status (4)

Country Link
US (1) US20050227231A1 (de)
AU (1) AU2002350485A1 (de)
DE (1) DE10246005A1 (de)
WO (1) WO2003031947A2 (de)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7297518B2 (en) 2001-03-12 2007-11-20 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US8703652B2 (en) 2009-11-06 2014-04-22 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US10722858B2 (en) 2013-03-15 2020-07-28 Lineage Biosciences, Inc. Methods and compositions for tagging and analyzing samples

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US8637650B2 (en) 2003-11-05 2014-01-28 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7295316B2 (en) * 2004-01-14 2007-11-13 Applera Corporation Fluorescent detector with automatic changing filters
CA2557177A1 (en) 2004-02-19 2005-09-01 Stephen Quake Methods and kits for analyzing polynucleotide sequences
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US8114973B2 (en) * 2004-05-25 2012-02-14 Helicos Biosciences Corporation Nucleotide analogs
JP2008512084A (ja) 2004-05-25 2008-04-24 ヘリコス バイオサイエンシーズ コーポレイション 核酸の配列決定のための方法およびデバイス
US20080026379A1 (en) * 2006-07-31 2008-01-31 Siddiqi Suhaib M Nucleotide analogs
US8071755B2 (en) * 2004-05-25 2011-12-06 Helicos Biosciences Corporation Nucleotide analogs
US20080103053A1 (en) * 2005-11-22 2008-05-01 Helicos Biosciences Corporation Methods and compositions for sequencing a nucleic acid
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US9976192B2 (en) 2006-03-10 2018-05-22 Ldip, Llc Waveguide-based detection system with scanning light source
US8675199B2 (en) 2006-03-10 2014-03-18 Plc Diagnostics, Inc. Waveguide-based detection system with scanning light source
US8288157B2 (en) 2007-09-12 2012-10-16 Plc Diagnostics, Inc. Waveguide-based optical scanning systems
US9528939B2 (en) 2006-03-10 2016-12-27 Indx Lifecare, Inc. Waveguide-based optical scanning systems
US9423397B2 (en) 2006-03-10 2016-08-23 Indx Lifecare, Inc. Waveguide-based detection system with scanning light source
EP2548972A1 (de) 2006-06-14 2013-01-23 Verinata Health, Inc Verfahren zur Diagnose fötaler Missbildungen
US20230076823A1 (en) 2007-01-26 2023-03-09 Illumina, Inc. Independently removable nucleic acid sequencing system and method
US7767805B2 (en) * 2007-05-03 2010-08-03 Helicos Biosciences Corporation Methods and compositions for sequencing a nucleic acid
US9163053B2 (en) * 2007-05-18 2015-10-20 Fluidigm Corporation Nucleotide analogs
GB2461026B (en) 2008-06-16 2011-03-09 Plc Diagnostics Inc System and method for nucleic acids sequencing by phased synthesis
WO2010091046A2 (en) * 2009-02-03 2010-08-12 President & Fellows Of Harvard College Systems and methods for high throughput, high fidelity, single molecule nucleic acid sequencing using time multiplexed excitation
CN102858995B (zh) 2009-09-10 2016-10-26 森特瑞隆技术控股公司 靶向测序方法
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
US9772272B2 (en) 2009-09-28 2017-09-26 Koninklijke Philips N.V. Substance determining apparatus
WO2012058634A2 (en) 2010-10-28 2012-05-03 Salk Institute For Biological Studies Epigenomic induced pluripotent stem cell signatures
CN103703143B (zh) 2011-01-31 2016-12-14 爱普瑞斯生物公司 鉴定细胞中的多个表位的方法
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
EP3836149A1 (de) 2011-11-07 2021-06-16 QIAGEN Redwood City, Inc. Verfahren und systeme zur identifizierung kausaler genomvarianten
JP5988629B2 (ja) * 2012-03-14 2016-09-07 オリンパス株式会社 複数の光学ユニットを備えた顕微鏡
WO2014026032A2 (en) 2012-08-08 2014-02-13 Apprise Bio, Inc. Increasing dynamic range for identifying multiple epitopes in cells
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
EP3885446A1 (de) 2013-02-01 2021-09-29 The Regents of The University of California Verfahren zur genomanordnung und haplotypen-phasierung
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
MX383494B (es) 2013-11-07 2025-03-14 Univ Leland Stanford Junior Ácidos nucleicos libres de células para el análisis del microbioma humano y componentes del mismo.
EP3540074A1 (de) 2013-12-11 2019-09-18 The Regents of the University of California Verfahren zur markierung innerer regionen von nukleinsäuremolekülen
US10018566B2 (en) 2014-02-28 2018-07-10 Ldip, Llc Partially encapsulated waveguide based sensing chips, systems and methods of use
CA2956925C (en) 2014-08-01 2024-02-13 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
NZ734854A (en) 2015-02-17 2022-11-25 Dovetail Genomics Llc Nucleic acid sequence assembly
WO2016138427A1 (en) 2015-02-27 2016-09-01 Indx Lifecare, Inc. Waveguide-based detection system with scanning light source
WO2016154540A1 (en) 2015-03-26 2016-09-29 Dovetail Genomics Llc Physical linkage preservation in dna storage
JP7300831B2 (ja) 2015-10-19 2023-06-30 ダブテイル ゲノミクス エルエルシー ゲノムアセンブリ、ハプロタイプフェージング、および標的に依存しない核酸検出のための方法
JP7441003B2 (ja) 2016-02-23 2024-02-29 ダブテイル ゲノミクス エルエルシー ゲノムアセンブリのためのフェージングされたリードセットの生成とハプロタイプフェージング
CN105861293B (zh) * 2016-04-06 2017-11-07 深圳市瀚海基因生物科技有限公司 单分子基因测序仪
KR102412442B1 (ko) 2016-05-13 2022-06-22 더브테일 제노믹스 엘엘씨 보존된 샘플로부터의 장범위 링키지 정보의 회수
CN108265004A (zh) * 2016-12-30 2018-07-10 广州康昕瑞基因健康科技有限公司 多通道测序反应小室的转接座
CA3060539A1 (en) 2017-04-18 2018-10-25 Dovetail Genomics, Llc Nucleic acid characteristics as guides for sequence assembly
WO2018237209A1 (en) 2017-06-21 2018-12-27 Bluedot Llc Systems and methods for identification of nucleic acids in a sample
WO2019060716A1 (en) 2017-09-25 2019-03-28 Freenome Holdings, Inc. SAMPLE EXTRACTION METHODS AND SYSTEMS
WO2019152543A1 (en) 2018-01-31 2019-08-08 Dovetail Genomics, Llc Sample prep for dna linkage recovery
CN112513242B (zh) * 2018-05-16 2024-04-30 深圳华大智造科技股份有限公司 基因测序仪
US11166996B2 (en) 2018-12-12 2021-11-09 Flagship Pioneering Innovations V, Inc. Anellovirus compositions and methods of use
AU2020302791A1 (en) 2019-06-27 2022-02-03 Dovetail Genomics, Llc Methods and compositions for proximity ligation
CN120457195A (zh) * 2022-12-27 2025-08-08 武汉华大智造科技有限公司 测序扫描系统、方法、测序仪及存储介质

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995000530A1 (en) * 1993-06-25 1995-01-05 Affymax Technologies N.V. Hybridization and sequencing of nucleic acids
EP1008845A1 (de) * 1997-07-10 2000-06-14 Ruprecht-Karls-Universität Heidelberg Wellenfeldmikroskop, Wellenfeldmikroskopieverfahren, auch zur DNA-Sequenzierung, und Kalibrierverfahren für die Wellenfeldmikroskopie
US6134002A (en) * 1999-01-14 2000-10-17 Duke University Apparatus and method for the rapid spectral resolution of confocal images
WO2000070073A1 (en) * 1999-05-19 2000-11-23 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6242193B1 (en) * 1999-07-30 2001-06-05 Hitachi, Ltd. Apparatus for determining base sequence of nucleic acid
US6245507B1 (en) * 1998-08-18 2001-06-12 Orchid Biosciences, Inc. In-line complete hyperspectral fluorescent imaging of nucleic acid molecules
WO2002088381A2 (de) * 2001-04-27 2002-11-07 Genovoxx Gmbh Verfahren zur bestimmung der genexpression
WO2002088382A2 (de) * 2001-04-27 2002-11-07 Genovoxx Gmbh Verfahren zur analyse von nukleinsäureketten

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5187085A (en) * 1990-09-28 1993-02-16 Applied Biosystems, Inc. Nucleic acid sequence analysis with nucleoside-5'-o-(1-thiotriphosphates)
EP0682671A4 (de) * 1993-02-01 1998-01-14 Seq Ltd Verfahren und geräte zur sequenzierung von dns.
US6132580A (en) * 1995-09-28 2000-10-17 The Regents Of The University Of California Miniature reaction chamber and devices incorporating same
US6524790B1 (en) * 1997-06-09 2003-02-25 Caliper Technologies Corp. Apparatus and methods for correcting for variable velocity in microfluidic systems
US6096496A (en) * 1997-06-19 2000-08-01 Frankel; Robert D. Supports incorporating vertical cavity emitting lasers and tracking apparatus for use in combinatorial synthesis
US5876675A (en) * 1997-08-05 1999-03-02 Caliper Technologies Corp. Microfluidic devices and systems
US6268146B1 (en) * 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6162602A (en) * 1998-07-16 2000-12-19 Gautsch; James W. Automatic direct sequencing of bases in nucleic acid chain elongation

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995000530A1 (en) * 1993-06-25 1995-01-05 Affymax Technologies N.V. Hybridization and sequencing of nucleic acids
EP1008845A1 (de) * 1997-07-10 2000-06-14 Ruprecht-Karls-Universität Heidelberg Wellenfeldmikroskop, Wellenfeldmikroskopieverfahren, auch zur DNA-Sequenzierung, und Kalibrierverfahren für die Wellenfeldmikroskopie
US6245507B1 (en) * 1998-08-18 2001-06-12 Orchid Biosciences, Inc. In-line complete hyperspectral fluorescent imaging of nucleic acid molecules
US6134002A (en) * 1999-01-14 2000-10-17 Duke University Apparatus and method for the rapid spectral resolution of confocal images
WO2000070073A1 (en) * 1999-05-19 2000-11-23 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6242193B1 (en) * 1999-07-30 2001-06-05 Hitachi, Ltd. Apparatus for determining base sequence of nucleic acid
WO2002088381A2 (de) * 2001-04-27 2002-11-07 Genovoxx Gmbh Verfahren zur bestimmung der genexpression
WO2002088382A2 (de) * 2001-04-27 2002-11-07 Genovoxx Gmbh Verfahren zur analyse von nukleinsäureketten

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7297518B2 (en) 2001-03-12 2007-11-20 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US11098350B2 (en) 2009-11-06 2021-08-24 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US9845497B2 (en) 2009-11-06 2017-12-19 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US10329607B2 (en) 2009-11-06 2019-06-25 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US10494669B2 (en) 2009-11-06 2019-12-03 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US10968479B2 (en) 2009-11-06 2021-04-06 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US10982275B2 (en) 2009-11-06 2021-04-20 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US8703652B2 (en) 2009-11-06 2014-04-22 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US11384389B2 (en) 2009-11-06 2022-07-12 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US11390918B2 (en) 2009-11-06 2022-07-19 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US11597966B2 (en) 2009-11-06 2023-03-07 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US10722858B2 (en) 2013-03-15 2020-07-28 Lineage Biosciences, Inc. Methods and compositions for tagging and analyzing samples
US11161087B2 (en) 2013-03-15 2021-11-02 Lineage Biosciences, Inc. Methods and compositions for tagging and analyzing samples

Also Published As

Publication number Publication date
WO2003031947A2 (de) 2003-04-17
US20050227231A1 (en) 2005-10-13
AU2002350485A1 (en) 2003-04-22
DE10246005A1 (de) 2003-04-30

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