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WO2003024199A3 - Production of transgenic avians using sperm-mediated transfection - Google Patents

Production of transgenic avians using sperm-mediated transfection Download PDF

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Publication number
WO2003024199A3
WO2003024199A3 PCT/US2002/030156 US0230156W WO03024199A3 WO 2003024199 A3 WO2003024199 A3 WO 2003024199A3 US 0230156 W US0230156 W US 0230156W WO 03024199 A3 WO03024199 A3 WO 03024199A3
Authority
WO
WIPO (PCT)
Prior art keywords
sperm
nucleic acid
avian
oocyte
transgenic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/030156
Other languages
French (fr)
Other versions
WO2003024199A2 (en
Inventor
Jeffrey C Rapp
Pramod Sutrave
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Synageva Biopharma Corp
Original Assignee
Avigenics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avigenics Inc filed Critical Avigenics Inc
Priority to AU2002341795A priority Critical patent/AU2002341795A1/en
Publication of WO2003024199A2 publication Critical patent/WO2003024199A2/en
Anticipated expiration legal-status Critical
Publication of WO2003024199A3 publication Critical patent/WO2003024199A3/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/90—Vector systems having a special element relevant for transcription from vertebrates avian

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to novel methods of producing transgenic avians, preferably chickens, wherein the incorporated transgene may be expressed as a constituent protein of the white of a hard-shell egg. The present invention provides sperm-mediated transfer for the introduction to an avian egg of a transgene encoding a heterologous polypeptide. The avian sperm may be irradiated before the transgenic gene is incorporated therein. Transgenic genes may be incorporated into avian sperm by lipofection, electroporation, restriction enzyme mediated integration (REMI) or similar methods. The modified avian sperm may then be delivered to an avian oocyte by microinjection, intracytoplasmic sperm injection (ICSI) or artificial insemination, or by natural coitus after the modified avian sperm are returned to a male bird. Heterologous nucleic acid may be integrated directly into the genomic nucleic acid of the oocyte or after first integrating the heterologous nucleic acid into the nucleic acid of a male germ cell and subsequent delivery of the transgenic male germ cell to an oocyte. Alternatively, the heterologous nucleic acid may be a episome within the sperm, or within the derivative zygote formed by the fusion of the sperm and the recipient oocyte, and may replicate independently of the zygote genome. Co-segregation of the episome with the replicated ooctye genome into all of the daughter cells may be induced by the heterologous nucleic acid having a centromeric body derived from, for example, a chromosome of a chicken.
PCT/US2002/030156 2001-09-21 2002-09-23 Production of transgenic avians using sperm-mediated transfection Ceased WO2003024199A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002341795A AU2002341795A1 (en) 2001-09-21 2002-09-23 Production of transgenic avians using sperm-mediated transfection

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32396101P 2001-09-21 2001-09-21
US32400101P 2001-09-21 2001-09-21
US60/323,961 2001-09-21
US60/324,001 2001-09-21

Publications (2)

Publication Number Publication Date
WO2003024199A2 WO2003024199A2 (en) 2003-03-27
WO2003024199A3 true WO2003024199A3 (en) 2004-07-01

Family

ID=26984208

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/030156 Ceased WO2003024199A2 (en) 2001-09-21 2002-09-23 Production of transgenic avians using sperm-mediated transfection

Country Status (2)

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AU (1) AU2002341795A1 (en)
WO (1) WO2003024199A2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US9150881B2 (en) 2009-04-09 2015-10-06 Proteovec Holding, L.L.C. Production of proteins using transposon-based vectors
US9150880B2 (en) 2008-09-25 2015-10-06 Proteovec Holding, L.L.C. Vectors for production of antibodies
US9157097B2 (en) 2008-09-25 2015-10-13 Proteovec Holding, L.L.C. Vectors for production of growth hormone
US10669562B2 (en) 2003-10-24 2020-06-02 Selexis S.A. High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE437233T1 (en) 2001-01-26 2009-08-15 Selexis Sa MATRIX ATTACHMENT REGIONS AND METHOD OF USE THEREOF
US20050034186A1 (en) * 2003-03-07 2005-02-10 Harvey Alex J. Site specific nucleic acid integration
KR100569168B1 (en) * 2003-08-08 2006-04-07 (주)아비코아생명공학연구소 Cultivation method of algae garden stem cells and algae garden stem cells obtained by
KR100569163B1 (en) * 2003-08-11 2006-04-07 (주)아비코아생명공학연구소 Production method of algae chimera using garden cells and algae chimera
DE602006014942D1 (en) 2005-03-04 2010-07-29 Celltrion Inc EXPRESSION VECTOR FOR ANIMAL CELL CONTAINING AT LEAST ONE COPY OF MAR DNA SEQUENCES AT THE 3 'END OF THE TRANSCRIPTION REFINING RANGE OF A GENE AND METHOD FOR EXPRESSING A FOREIGN AGGREGATION USING THE VECTOR
WO2011015917A2 (en) * 2009-08-03 2011-02-10 Avesthagen Limited Vectors and compounds for expression of recombinant tnk-tpa (tenecteplase)
WO2011015918A2 (en) * 2009-08-03 2011-02-10 Avesthagen Limited Vectors and compounds for expression of recombinant cetuximab
WO2011015924A2 (en) * 2009-08-03 2011-02-10 Avesthagen Limited Vectors and compounds for expression of recombinant trastuzumab
WO2011015916A2 (en) * 2009-08-03 2011-02-10 Avesthagen Limited Vectors and compounds for expression of recombinant infliximab
US20150024497A1 (en) * 2013-07-19 2015-01-22 Elwha Llc Methods and Systems for Utilizing Sperm for Molecular Delivery
AU2016305490B2 (en) * 2015-08-07 2022-07-14 Commonwealth Scientific And Industrial Research Organisation Method for producing an animal comprising a germline genetic modification
KR20200088805A (en) * 2017-09-19 2020-07-23 더 스테이트 오브 이스라엘, 미니스트리 오브 애그리컬처 & 루럴 디벨로프먼트, 애그리컬처럴 리서치 오거니제이션, (에이.알.오.), 볼카니 센터 Genome-editing algae
CN118064501B (en) * 2024-02-21 2025-02-18 浙江大学 A method for introducing exogenous nucleic acid fragments into bivalve sperm

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US7608451B2 (en) 2002-06-26 2009-10-27 Transgen Rx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US10669562B2 (en) 2003-10-24 2020-06-02 Selexis S.A. High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences
US9150880B2 (en) 2008-09-25 2015-10-06 Proteovec Holding, L.L.C. Vectors for production of antibodies
US9157097B2 (en) 2008-09-25 2015-10-13 Proteovec Holding, L.L.C. Vectors for production of growth hormone
US9150881B2 (en) 2009-04-09 2015-10-06 Proteovec Holding, L.L.C. Production of proteins using transposon-based vectors

Also Published As

Publication number Publication date
WO2003024199A2 (en) 2003-03-27
AU2002341795A1 (en) 2003-04-01

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