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WO2003020877A3 - Staining and sorting genomes and chromosomes using sequence-specific polyamides - Google Patents

Staining and sorting genomes and chromosomes using sequence-specific polyamides Download PDF

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Publication number
WO2003020877A3
WO2003020877A3 PCT/US2002/021841 US0221841W WO03020877A3 WO 2003020877 A3 WO2003020877 A3 WO 2003020877A3 US 0221841 W US0221841 W US 0221841W WO 03020877 A3 WO03020877 A3 WO 03020877A3
Authority
WO
WIPO (PCT)
Prior art keywords
sorting
chromosomes
staining
sequence
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/021841
Other languages
French (fr)
Other versions
WO2003020877A2 (en
Inventor
Bradley Didion
James Bashkin
Scott Woodard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pharmacia LLC
Original Assignee
Pharmacia LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia LLC filed Critical Pharmacia LLC
Priority to AU2002320399A priority Critical patent/AU2002320399A1/en
Publication of WO2003020877A2 publication Critical patent/WO2003020877A2/en
Publication of WO2003020877A3 publication Critical patent/WO2003020877A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6879—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Genomic DNA of reproductive cells, including sperm and other cells important in reproduction, are stained and optionally sorted by targeting oligomeric polyamides capable of fluorescence to target DNA sequences and then sorting the cells, for example, by fluorescence activated cell sorting or other cell separation techniques.
PCT/US2002/021841 2001-09-01 2002-08-15 Staining and sorting genomes and chromosomes using sequence-specific polyamides Ceased WO2003020877A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002320399A AU2002320399A1 (en) 2001-09-01 2002-08-15 Staining and sorting genomes and chromosomes using sequence-specific polyamides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31672901P 2001-09-01 2001-09-01
US60/316,729 2001-09-01

Publications (2)

Publication Number Publication Date
WO2003020877A2 WO2003020877A2 (en) 2003-03-13
WO2003020877A3 true WO2003020877A3 (en) 2003-12-11

Family

ID=23230389

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/021841 Ceased WO2003020877A2 (en) 2001-09-01 2002-08-15 Staining and sorting genomes and chromosomes using sequence-specific polyamides

Country Status (2)

Country Link
AU (1) AU2002320399A1 (en)
WO (1) WO2003020877A2 (en)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4323571B2 (en) 1997-01-31 2009-09-02 エックスワイ， インコーポレイテッド Optical device
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6071689A (en) 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
CA2408939C (en) 2000-05-09 2011-11-08 Xy, Inc. High purity x-chromosome bearing and y-chromosome bearing populations of spermatozoa
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
WO2002043574A2 (en) 2000-11-29 2002-06-06 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
US8211629B2 (en) 2002-08-01 2012-07-03 Xy, Llc Low pressure sperm cell separation system
CA2534394C (en) 2002-08-15 2013-01-08 Xy, Inc. High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
DK2308419T3 (en) 2003-03-28 2016-06-06 Inguran Llc Procedure for assessing the staining conditions of animal sperm to be sorted.
EP1625203B1 (en) 2003-05-15 2015-04-08 Xy, Llc Efficient haploid cell sorting for flow cytometer systems
ZA200603288B (en) 2003-10-07 2007-09-26 Univ Western Sydney Sequence selective pyrrole and imidazole polyamide metallocomplexes
WO2005095590A2 (en) 2004-03-29 2005-10-13 Monsanto Technology Llc Sperm suspensions for sorting into x or y chromosome-bearing enriched populations
AR049732A1 (en) 2004-07-22 2006-08-30 Monsanto Co PROCESS TO ENRICH A Sperm Cell Population
US7618770B2 (en) 2005-07-29 2009-11-17 Xy, Inc. Methods and apparatus for reducing protein content in sperm cell extenders
US8521440B2 (en) 2007-01-08 2013-08-27 The Invention Science Fund I, Llc Systems for genome selection
US7709244B2 (en) 2007-01-08 2010-05-04 The Invention Science Fund I, Llc Systems for genome selection
US7713728B2 (en) 2007-01-08 2010-05-11 The Invention Sciencefund I, Llc Systems for genome selection
US7947457B2 (en) 2007-01-08 2011-05-24 The Invention Science Fund I, Llc Systems for genome selection
US7709245B2 (en) 2007-01-08 2010-05-04 The Invention Science Fund I, Llc Systems for genome selection
US7888036B2 (en) 2007-01-08 2011-02-15 The Invention Science Fund I, Llc Systems for genome selection
US7718418B2 (en) 2007-01-08 2010-05-18 The Invention Science Fund I, Llc Systems for genome selection
US7648823B2 (en) 2007-01-08 2010-01-19 Searete Llc Systems for genome selection
US7947455B2 (en) 2007-01-08 2011-05-24 The Invention Science Fund I, Llc System for genome selection
US7985578B2 (en) 2007-01-08 2011-07-26 The Invention Science Fund I, Llc Systems for genome selection
US8012740B2 (en) 2007-01-08 2011-09-06 The Invention Science Fund I, Llc Systems for genome selection
EP2229457A4 (en) * 2007-12-14 2011-01-26 Minitube America Inc Gender-specific separation of sperm cells and embryos
DK3492603T3 (en) 2012-10-05 2022-10-17 Inguran Llc METHODS OF PROCESSING SPERM FOR SEX SORTING

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BITO ET AL.: "Differences in the DNA-stainability of spermatozoa from fertile and suspected infertile men", INTERNATIONAL JOURNAL OF ANDROLOGY, vol. 22, 1999, pages 275 - 281, XP002967851 *

Also Published As

Publication number Publication date
AU2002320399A1 (en) 2003-03-18
WO2003020877A2 (en) 2003-03-13

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