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WO2003000920A3 - Procedes de caracterisation de molecules d'acide nucleique - Google Patents

Procedes de caracterisation de molecules d'acide nucleique Download PDF

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Publication number
WO2003000920A3
WO2003000920A3 PCT/US2002/019766 US0219766W WO03000920A3 WO 2003000920 A3 WO2003000920 A3 WO 2003000920A3 US 0219766 W US0219766 W US 0219766W WO 03000920 A3 WO03000920 A3 WO 03000920A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
local
acid molecule
modified
molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/019766
Other languages
English (en)
Other versions
WO2003000920A9 (fr
WO2003000920A2 (fr
Inventor
Daniel Branton
Hui Wang
Stephen Laderman
Jeffrey Sampson
Zohar Yakhani
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agilent Technologies Inc
Harvard University
Original Assignee
Agilent Technologies Inc
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agilent Technologies Inc, Harvard University filed Critical Agilent Technologies Inc
Priority to EP02747942A priority Critical patent/EP1478771A4/fr
Priority to AU2002318386A priority patent/AU2002318386A1/en
Publication of WO2003000920A2 publication Critical patent/WO2003000920A2/fr
Publication of WO2003000920A9 publication Critical patent/WO2003000920A9/fr
Anticipated expiration legal-status Critical
Publication of WO2003000920A3 publication Critical patent/WO2003000920A3/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N7/00—Television systems
    • H04N7/18—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Multimedia (AREA)
  • Signal Processing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Selon l'invention, une zone locale transversale, une charge locale ou la chimie locale d'une molécule est modifiée en vue de sonder les caractéristiques d'une molécule d'acide nucléique cible ou d'une population de molécules d'acide nucléique cibles. L'acide nucléique modifié est mis en contact avec un substrat comprenant un détecteur répondant à la modification dans la zone locale transversale, dans la charge locale ou dans la chimie locale de la molécule d'acide nucléique. La molécule d'acide nucléique modifiée traverse un volume défini et, de préférence, aux dimensions moléculaires, sur le substrat, de manière que des nucléotides de la molécule d'acide nucléique modifiée interagissent avec le détecteur dans un ordre séquentiel, des données en corrélation avec la zone transversale, la charge locale ou la chimie locale de la molécule d'acide nucléique étant obtenues. La molécule d'acide nucléique peut être modifiée au niveau de plusieurs emplacements situés le long de la longueur de la molécule. L'interaction des nucléotides individuels de la molécule d'acide nucléique avec le détecteur dans un ordre séquentiel permet d'obtenir des informations relatives à l'emplacement et à la composition d'une pluralité de sites modifiés le long d'une molécule unique.
PCT/US2002/019766 2001-06-21 2002-06-21 Procedes de caracterisation de molecules d'acide nucleique Ceased WO2003000920A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02747942A EP1478771A4 (fr) 2001-06-21 2002-06-21 Procedes de caracterisation de molecules d'acide nucleique
AU2002318386A AU2002318386A1 (en) 2001-06-21 2002-06-21 Methods for characterization of nucleic acid molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29987001P 2001-06-21 2001-06-21
US60/299,870 2001-06-21

Publications (3)

Publication Number Publication Date
WO2003000920A2 WO2003000920A2 (fr) 2003-01-03
WO2003000920A9 WO2003000920A9 (fr) 2003-03-20
WO2003000920A3 true WO2003000920A3 (fr) 2004-09-23

Family

ID=23156656

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/019766 Ceased WO2003000920A2 (fr) 2001-06-21 2002-06-21 Procedes de caracterisation de molecules d'acide nucleique

Country Status (3)

Country Link
EP (1) EP1478771A4 (fr)
AU (1) AU2002318386A1 (fr)
WO (1) WO2003000920A2 (fr)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US20120160687A1 (en) 1995-03-17 2012-06-28 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US7410564B2 (en) * 2003-01-27 2008-08-12 Agilent Technologies, Inc. Apparatus and method for biopolymer identification during translocation through a nanopore
US7282130B2 (en) 2003-01-31 2007-10-16 Agilent Technologies, Inc. Apparatus and method for control of biopolymer translocation through a nanopore
US7846738B2 (en) 2003-08-15 2010-12-07 President And Fellows Of Harvard College Study of polymer molecules and conformations with a nanopore
US7238485B2 (en) 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
GB0713143D0 (en) * 2007-07-06 2007-08-15 Ucl Business Plc Nucleic acid detection method
EP2201136B1 (fr) 2007-10-01 2017-12-06 Nabsys 2.0 LLC Séquençage par nanopore et hybridation de sondes pour former des complexes ternaires et l'alignement de plage variable
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
JP5717634B2 (ja) 2008-09-03 2015-05-13 ナブシス， インコーポレイテッド 流体チャネル内の生体分子および他の分析物の電圧感知のための、長手方向に変位されるナノスケールの電極の使用
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US20100243449A1 (en) * 2009-03-27 2010-09-30 Oliver John S Devices and methods for analyzing biomolecules and probes bound thereto
AU2010326349B2 (en) 2009-12-01 2015-10-29 Oxford Nanopore Technologies Limited Biochemical analysis instrument
US9005425B2 (en) 2010-03-05 2015-04-14 University Of Utah Research Foundation Detection of nucleic acid lesions and adducts using nanopores
EP2580588B1 (fr) 2010-06-08 2014-09-24 President and Fellows of Harvard College Dispositif à nanopores avec membrane lipidique artificielle sur support de graphène
CA2805247C (fr) 2010-07-14 2021-08-10 The Curators Of The University Of Missouri Detection de molecules individuelles d'acides nucleiques facilitee par des nanopores
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
EP2622343B1 (fr) 2010-10-01 2016-01-20 Oxford Nanopore Technologies Limited Appareil d'analyse biochimique et vanne rotative
EP2640849B1 (fr) 2010-11-16 2016-04-06 Nabsys 2.0 LLC Procédés de séquençage d'une biomolécule par détection de positions relatives de sondes hybridées
US11274341B2 (en) 2011-02-11 2022-03-15 NABsys, 2.0 LLC Assay methods using DNA binding proteins
WO2012177792A2 (fr) 2011-06-24 2012-12-27 Sequenom, Inc. Méthodes et procédés pour estimation non invasive de variation génétique
GB2498021A (en) * 2011-09-07 2013-07-03 Univ Utah Res Found Detection of Nucleic acid lesions and adducts using nanopores
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
US20140242588A1 (en) 2011-10-06 2014-08-28 Sequenom, Inc Methods and processes for non-invasive assessment of genetic variations
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US8688388B2 (en) 2011-10-11 2014-04-01 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
PL2805280T3 (pl) 2012-01-20 2022-11-21 Sequenom, Inc. Procesy diagnostyczne będące czynnikiem warunków doświadczalnych
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
WO2014113557A1 (fr) 2013-01-18 2014-07-24 Nabsys, Inc. Liaison améliorée d'une sonde
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2971118B1 (fr) 2013-03-15 2019-10-02 The Curators of the University of Missouri Capteur à nanopores codés pour la détection multiplexée d'acides nucléiques
US10930368B2 (en) 2013-04-03 2021-02-23 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP3578670B1 (fr) 2013-05-24 2025-07-02 Sequenom, Inc. Méthodes et procédés d'évaluation non invasive de variations génétiques
KR102447079B1 (ko) 2013-06-21 2022-09-23 시쿼넘, 인코포레이티드 유전적 변이의 비침습 평가를 위한 방법 및 프로세스
AU2014329493B2 (en) 2013-10-04 2020-09-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP3495496B1 (fr) 2013-10-07 2020-11-25 Sequenom, Inc. Méthodes et procédés d'évaluation non invasive d'altérations chromosomiques
US20160034640A1 (en) 2014-07-30 2016-02-04 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2016154337A2 (fr) * 2015-03-23 2016-09-29 The University Of North Carolina At Chapel Hill Procédé d'identification et d'énumération de séquences d'acide nucléique, expression, variant d'épissage, translocation, copie ou changement de méthylation d'adn utilisant des réactions associant nucléase, ligase, polymérase, transférase terminale et séquençage
EP3491560A1 (fr) 2016-07-27 2019-06-05 Sequenom, Inc. Classifications de modifications du nombre de copies génétiques
EP3574424A1 (fr) 2017-01-24 2019-12-04 Sequenom, Inc. Méthodes et procédés d'évaluation de variations génétiques
EP3998350B1 (fr) 2017-03-17 2025-05-07 Sequenom, Inc. Procédés et techniques d'évaluation d'un mosaïcisme génétique

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US20030104428A1 (en) * 2001-06-21 2003-06-05 President And Fellows Of Harvard College Method for characterization of nucleic acid molecules

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6362002B1 (en) * 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
WO1998035012A2 (fr) * 1997-02-12 1998-08-13 Chan Eugene Y Procedes et produits permettant d'analyser des polymeres
US6537755B1 (en) * 1999-03-25 2003-03-25 Radoje T. Drmanac Solution-based methods and materials for sequence analysis by hybridization
EP1192103A1 (fr) * 1999-06-22 2002-04-03 President And Fellows of Harvard College Procede permettant de respecter les caracteristiques dimensionnelles d'une structure a l'etat solide
ATE316582T1 (de) * 1999-09-07 2006-02-15 Univ California Verfahren um die anwesenheit von doppelsträngiger dns in einer probe nachzuweisen
WO2002042496A2 (fr) * 2000-11-27 2002-05-30 The Regents Of The University Of California Procedes et dispositifs de caracterisation de molecules d'acide nucleique bicatenaire

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US20030104428A1 (en) * 2001-06-21 2003-06-05 President And Fellows Of Harvard College Method for characterization of nucleic acid molecules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1478771A4 *

Also Published As

Publication number Publication date
AU2002318386A1 (en) 2003-01-08
EP1478771A2 (fr) 2004-11-24
WO2003000920A9 (fr) 2003-03-20
EP1478771A4 (fr) 2005-06-15
WO2003000920A2 (fr) 2003-01-03

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