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WO2003000839A3 - Clinical assay for nucleic acids amplified in solid matrices to produce colonies of the progeny of individual target molecules - Google Patents

Clinical assay for nucleic acids amplified in solid matrices to produce colonies of the progeny of individual target molecules Download PDF

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Publication number
WO2003000839A3
WO2003000839A3 PCT/RU2002/000307 RU0200307W WO03000839A3 WO 2003000839 A3 WO2003000839 A3 WO 2003000839A3 RU 0200307 W RU0200307 W RU 0200307W WO 03000839 A3 WO03000839 A3 WO 03000839A3
Authority
WO
WIPO (PCT)
Prior art keywords
progeny
target molecules
nucleic acids
target
individual target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/RU2002/000307
Other languages
French (fr)
Other versions
WO2003000839A2 (en
Inventor
Helena V Chetverina
Alexander B Chetverin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INSTITUT BELKA OF RUSSIAN FEDERATION
INST BELKA OF RUSSIAN FEDE
Original Assignee
INSTITUT BELKA OF RUSSIAN FEDERATION
INST BELKA OF RUSSIAN FEDE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by INSTITUT BELKA OF RUSSIAN FEDERATION, INST BELKA OF RUSSIAN FEDE filed Critical INSTITUT BELKA OF RUSSIAN FEDERATION
Priority to EP02768208A priority Critical patent/EP1409744A4/en
Priority to AU2002330802A priority patent/AU2002330802A1/en
Publication of WO2003000839A2 publication Critical patent/WO2003000839A2/en
Publication of WO2003000839A3 publication Critical patent/WO2003000839A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A quantitative multiplex assay for at least two nucleic acid targets, on of which is rare and another of which is present at much higher level, comprising adding reagents for amplification of the targets by polymerase chain reaction (PCR) to a thin polyacrylamide gel matrix that has been washed and dried, distributing a sample containing the targets across the surface of the gel sufficiently to separate rare target molecules but not molecules of the abundant target, cycling the gel through a PCR protocol to amplify the target, and counting the number of individual colonies of the rare target.
PCT/RU2002/000307 2001-06-21 2002-06-21 Clinical assay for nucleic acids amplified in solid matrices to produce colonies of the progeny of individual target molecules Ceased WO2003000839A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02768208A EP1409744A4 (en) 2001-06-21 2002-06-21 Clinical assay for nucleic acids amplified in solid matrices to produce colonies of the progeny of individual target molecules
AU2002330802A AU2002330802A1 (en) 2001-06-21 2002-06-21 Clinical assay for nucleic acids amplified in solid matrices to produce colonies of the progeny of individual target molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30002401P 2001-06-21 2001-06-21
US60/300,024 2001-06-21

Publications (2)

Publication Number Publication Date
WO2003000839A2 WO2003000839A2 (en) 2003-01-03
WO2003000839A3 true WO2003000839A3 (en) 2003-03-13

Family

ID=23157342

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/RU2002/000307 Ceased WO2003000839A2 (en) 2001-06-21 2002-06-21 Clinical assay for nucleic acids amplified in solid matrices to produce colonies of the progeny of individual target molecules

Country Status (3)

Country Link
EP (1) EP1409744A4 (en)
AU (1) AU2002330802A1 (en)
WO (1) WO2003000839A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004099439A1 (en) * 2003-05-09 2004-11-18 Tsinghua University Methods and compositions for optimizing multiplex pcr primers
JP4777631B2 (en) * 2004-09-27 2011-09-21 株式会社日立ハイテクノロジーズ Nucleic acid amplification analysis method and apparatus
RU2394915C2 (en) * 2006-03-24 2010-07-20 Александр Борисович Четверин Non-contact methods of detecting molecular colonies, sets of reagents and device for realising said methods
US7534588B2 (en) * 2007-05-25 2009-05-19 Asiagen Corporation Methods, kits and polynucleotides for simultaneously diagnosing viruses
WO2008157316A1 (en) * 2007-06-14 2008-12-24 Creighton University Improved method for rna isolation
CN107858404A (en) * 2017-12-22 2018-03-30 嘉兴雅康博贝南生物科技有限公司 It is a kind of for the neck ring primer of multiplexed PCR amplification and its application

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2114915C1 (en) * 1993-09-14 1998-07-10 Институт белка РАН Method of identification of nucleic acid
RU2161199C1 (en) * 2000-03-24 2000-12-27 Московский НИИ педиатрии и детской хирургии Recombinant plasmid dna pyai 960 designated for human 18-th chromosome marking
WO2001036442A1 (en) * 1999-11-17 2001-05-25 Jiuping Ji Simultaneous detection of hbv, hcv and hiv in plasma samples using a multiplex capture assay

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6001568A (en) * 1992-10-26 1999-12-14 Institut Belka Solid medium for amplification and expression of nucleic acids as colonies

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2114915C1 (en) * 1993-09-14 1998-07-10 Институт белка РАН Method of identification of nucleic acid
WO2001036442A1 (en) * 1999-11-17 2001-05-25 Jiuping Ji Simultaneous detection of hbv, hcv and hiv in plasma samples using a multiplex capture assay
RU2161199C1 (en) * 2000-03-24 2000-12-27 Московский НИИ педиатрии и детской хирургии Recombinant plasmid dna pyai 960 designated for human 18-th chromosome marking

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1409744A4 *

Also Published As

Publication number Publication date
EP1409744A2 (en) 2004-04-21
AU2002330802A1 (en) 2003-01-08
WO2003000839A2 (en) 2003-01-03
EP1409744A4 (en) 2004-11-03

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