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WO2003097669A3 - Method for purifying denatured proteins having a desired disulfide bond configuration - Google Patents

Method for purifying denatured proteins having a desired disulfide bond configuration Download PDF

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Publication number
WO2003097669A3
WO2003097669A3 PCT/DK2003/000324 DK0300324W WO03097669A3 WO 2003097669 A3 WO2003097669 A3 WO 2003097669A3 DK 0300324 W DK0300324 W DK 0300324W WO 03097669 A3 WO03097669 A3 WO 03097669A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
disulfide bond
bond configuration
purifying
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DK2003/000324
Other languages
French (fr)
Other versions
WO2003097669A2 (en
Inventor
Soeren Buus
Henrik Ferre
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Københavns Universitet
Original Assignee
Københavns Universitet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Københavns Universitet filed Critical Københavns Universitet
Priority to AU2003223936A priority Critical patent/AU2003223936A1/en
Publication of WO2003097669A2 publication Critical patent/WO2003097669A2/en
Publication of WO2003097669A3 publication Critical patent/WO2003097669A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to a method for production of a protein having a desired fold. This is especially achieved by subjecting a population of proteins to a separation step under non-reducing conditions. This allows for identification of a sub-population of proteins having the disulfide bond configuration resulting in a desired fold. Most often this will be the protein of proper structure and/or function. Thus, by using the novel method the purity of the protein having a desired fold can be increased as compared to the purity of a similar protein produced by a conventional method. Important aspect of the invention is a functional active MHC heavy chain protein obtainable by the above method and the use of a MHC heavy chain protein in analysis of peptide binding capacity.
PCT/DK2003/000324 2002-05-17 2003-05-15 Method for purifying denatured proteins having a desired disulfide bond configuration Ceased WO2003097669A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003223936A AU2003223936A1 (en) 2002-05-17 2003-05-15 Method for purifying denatured proteins having a desired disulfide bond configuration

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200200766 2002-05-17
DKPA200200766 2002-05-17

Publications (2)

Publication Number Publication Date
WO2003097669A2 WO2003097669A2 (en) 2003-11-27
WO2003097669A3 true WO2003097669A3 (en) 2004-03-18

Family

ID=29433048

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2003/000324 Ceased WO2003097669A2 (en) 2002-05-17 2003-05-15 Method for purifying denatured proteins having a desired disulfide bond configuration

Country Status (2)

Country Link
AU (1) AU2003223936A1 (en)
WO (1) WO2003097669A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2487673C (en) * 2003-12-02 2010-11-02 F. Hoffmann-La Roche Ag Improved method for the recombinant production and purification of protein kinases
US20060121055A1 (en) * 2004-12-06 2006-06-08 Becton, Dickinson And Company, Inc. Compositions with enhanced immunogenicity
CN101921216B (en) * 2010-09-08 2013-03-13 天津大学 Small molecular compound N-alkyl acyl cystamine for simulating function of folding enzyme, a preparation method thereof and method for assisting protein oxidizing and refolding
GB201506870D0 (en) * 2015-04-22 2015-06-03 Ucb Biopharma Sprl Method
WO2023141224A2 (en) * 2022-01-19 2023-07-27 University Of Southern California Methods for making disulfide-rich peptides and proteins

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988008849A1 (en) * 1987-05-11 1988-11-17 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
WO1993000107A1 (en) * 1991-06-20 1993-01-07 Rhone-Poulenc Rorer International (Holdings) Inc. Therapeutic fragments of von willebrand factor
EP0529086A1 (en) * 1991-02-26 1993-03-03 Ajinomoto Co., Inc. Processes for purifying human bcdf
WO1996003425A1 (en) * 1994-07-25 1996-02-08 Ciba-Geigy Ag Process for folding of proteins like recombinant hirudin or epidermal growth factor
WO2000015665A2 (en) * 1998-09-14 2000-03-23 Pedersen Lars Oestergaard A method of producing a functional immunoglobulin superfamily protein

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988008849A1 (en) * 1987-05-11 1988-11-17 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
EP0529086A1 (en) * 1991-02-26 1993-03-03 Ajinomoto Co., Inc. Processes for purifying human bcdf
WO1993000107A1 (en) * 1991-06-20 1993-01-07 Rhone-Poulenc Rorer International (Holdings) Inc. Therapeutic fragments of von willebrand factor
WO1996003425A1 (en) * 1994-07-25 1996-02-08 Ciba-Geigy Ag Process for folding of proteins like recombinant hirudin or epidermal growth factor
WO2000015665A2 (en) * 1998-09-14 2000-03-23 Pedersen Lars Oestergaard A method of producing a functional immunoglobulin superfamily protein

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
D N GARBOCZI ET AL.: "HLA-A2 peptide complexes: refolding and crystallization of molecules expressed in E. coli and complexed with single antigenic peptides", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 89, no. 4, 1992, NATIONAL ACADEMY OF SCIENCE. WASHINGTON., US, pages 3429 - 3433, XP002131059, ISSN: 0027-8424 *
L O PEDERSEN ET AL.: "Efficient assembly of recombinant major histocompatibility complex class I molecules with preformed disulfide bonds", EUROPEAN JOURNAL OF IMMUNOLOGY, vol. 31, 2001, WEINHEIM, DE, pages 2986 - 2996, XP002218800, ISSN: 0014-2980 *
L OWENS NARHI ET AL.: "Circular dichroism of reduced and oxidized recombinant human epidermal growth factor", INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH., vol. 39, 1992, MUNKSGAARD, COPENHAGEN., DK, pages 182 - 187, XP002218799, ISSN: 0367-8377 *

Also Published As

Publication number Publication date
AU2003223936A1 (en) 2003-12-02
WO2003097669A2 (en) 2003-11-27

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