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WO2003082904A3 - Modified aequorins having enhanced glowing kinetics and methods of using same - Google Patents

Modified aequorins having enhanced glowing kinetics and methods of using same Download PDF

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Publication number
WO2003082904A3
WO2003082904A3 PCT/US2003/007979 US0307979W WO03082904A3 WO 2003082904 A3 WO2003082904 A3 WO 2003082904A3 US 0307979 W US0307979 W US 0307979W WO 03082904 A3 WO03082904 A3 WO 03082904A3
Authority
WO
WIPO (PCT)
Prior art keywords
glowing
methods
modified
polypeptides
enhanced
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/007979
Other languages
French (fr)
Other versions
WO2003082904A2 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Millipore Corp
Original Assignee
Chemicon International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chemicon International Inc filed Critical Chemicon International Inc
Priority to AU2003224694A priority Critical patent/AU2003224694A1/en
Publication of WO2003082904A2 publication Critical patent/WO2003082904A2/en
Publication of WO2003082904A3 publication Critical patent/WO2003082904A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Modified apoaequorin polypeptides that exhibit enhanced glowing are provided, as are functional fragments of the polypeptides, and polynucleotides encoding the polypeptides and functional fragments. A modified glowing aequorin photoprotein also is provided. In addition, methods of using the glowing apoaequorins and encoding polypeptides to detect, for example, the presence of calcium ions in a sample, or to identify agents that effect the movement of calcium ions from one compartment to another are provided, as are methods for identifying functional changes in cells associated with changes in calcium ion concentrations.
PCT/US2003/007979 2002-03-25 2003-03-14 Modified aequorins having enhanced glowing kinetics and methods of using same Ceased WO2003082904A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003224694A AU2003224694A1 (en) 2002-03-25 2003-03-14 Modified aequorins having enhanced glowing kinetics and methods of using same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10754502A 2002-03-25 2002-03-25
US10/107,545 2002-03-25

Publications (2)

Publication Number Publication Date
WO2003082904A2 WO2003082904A2 (en) 2003-10-09
WO2003082904A3 true WO2003082904A3 (en) 2004-03-11

Family

ID=28673575

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/007979 Ceased WO2003082904A2 (en) 2002-03-25 2003-03-14 Modified aequorins having enhanced glowing kinetics and methods of using same

Country Status (2)

Country Link
AU (1) AU2003224694A1 (en)
WO (1) WO2003082904A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7396655B2 (en) 2005-03-30 2008-07-08 Chisso Corporation Method for enhancing activity of luciferase with fluorescence activity
US9678067B2 (en) 2005-04-28 2017-06-13 Jnc Corporation Method for extending light-emitting time of calcium-binding photoprotein solution

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITMI20040598A1 (en) * 2004-03-26 2004-06-26 Carlotta Giorgi METHOD FOR DETECTION OF INTRACELLULAR PARAMETERS WITH LUMINESCENT PROTEIN PROBES FOR THE SCREENING OF MOLECULES ABLE TO ALTER THE SAID PARAMETERS
GB0625678D0 (en) * 2006-12-21 2007-01-31 Lux Biotechnology Ltd Composition and method for detection of demineralisation
JP5793921B2 (en) 2011-04-05 2015-10-14 Jnc株式会社 Mutated apoprotein of low calcium sensitive photoprotein
JP2019500849A (en) * 2015-10-30 2019-01-17 サーントル ナシオナル ドゥ ラ ルシェルシュ シャーンティフィク （セエンエールエス） Gene-mutated aequorin-based biosensor with special calcium sensitivity and improved bioluminescence intensity
GB201700317D0 (en) 2017-01-09 2017-02-22 Calcivis Ltd Detection device
USD1042180S1 (en) 2020-05-28 2024-09-17 Calcivis Limited Dental detection device

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5876995A (en) * 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
US6232107B1 (en) * 1998-03-27 2001-05-15 Bruce J. Bryan Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5876995A (en) * 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
US6232107B1 (en) * 1998-03-27 2001-05-15 Bruce J. Bryan Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WHITE ET AL.: "Signal amplification system for DNA hybridization assays based on in vitro expression of a DNA label encoding apoaequorin", NUCL. ACIDS RES., vol. 27, 1999, pages I - VIII, XP002967866 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7396655B2 (en) 2005-03-30 2008-07-08 Chisso Corporation Method for enhancing activity of luciferase with fluorescence activity
US9678067B2 (en) 2005-04-28 2017-06-13 Jnc Corporation Method for extending light-emitting time of calcium-binding photoprotein solution

Also Published As

Publication number Publication date
WO2003082904A2 (en) 2003-10-09
AU2003224694A1 (en) 2003-10-13
AU2003224694A8 (en) 2003-10-13

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