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WO2003080660A3 - Procede de preparation d'une proteine de liaison a l'heparine (hbp) mammifere recombinee - Google Patents

Procede de preparation d'une proteine de liaison a l'heparine (hbp) mammifere recombinee Download PDF

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Publication number
WO2003080660A3
WO2003080660A3 PCT/DK2003/000207 DK0300207W WO03080660A3 WO 2003080660 A3 WO2003080660 A3 WO 2003080660A3 DK 0300207 W DK0300207 W DK 0300207W WO 03080660 A3 WO03080660 A3 WO 03080660A3
Authority
WO
WIPO (PCT)
Prior art keywords
hbp
preparation
binding protein
fusion protein
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DK2003/000207
Other languages
English (en)
Other versions
WO2003080660A2 (fr
Inventor
Helle Fabricius Woeldike
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leukotech AS
Original Assignee
Leukotech AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leukotech AS filed Critical Leukotech AS
Priority to AU2003226907A priority Critical patent/AU2003226907A1/en
Publication of WO2003080660A2 publication Critical patent/WO2003080660A2/fr
Publication of WO2003080660A3 publication Critical patent/WO2003080660A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4723—Cationic antimicrobial peptides, e.g. defensins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne des procédés de préparation d'une protéine de liaison à l'héparine (HBP) dans un système d'expression bactérien recombiné. Plus particulièrement, l'invention concerne un procédé de préparation d'une protéine de fusion insoluble comprenant une protéine de liaison à l'héparine (HBP), un site de clivage protéolytique, et un second polypeptide dans des cellules bactériennes recombinées, ladite protéine de fusion étant accumulée dans des corps d'inclusion siégeant dans le cytoplasme d'une bactérie après l'expression. Par ailleurs, l'invention concerne des procédés de séparation de la HBP exprimée de corps d'inclusion qui consistent à purifier la HBP du second polypeptide, et éventuellement, replier ladite HBP. De plus, l'invention concerne des produits de recombinaison d'ADN qui comprennent différentes protéines de fusion HBP. L'invention concerne enfin l'utilisation de la protéine de fusion HBP d'origine bactérienne dans la production de HBP pure, ainsi que l'utilisation de la HBP purifiée d'origine bactérienne dans la préparation d'un médicament.
PCT/DK2003/000207 2002-03-27 2003-03-26 Procede de preparation d'une proteine de liaison a l'heparine (hbp) mammifere recombinee Ceased WO2003080660A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003226907A AU2003226907A1 (en) 2002-03-27 2003-03-26 Method for the preparation of recombinant mammalian heparin-binding protein (hbp)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200200477 2002-03-27
DKPA200200477 2002-03-27

Publications (2)

Publication Number Publication Date
WO2003080660A2 WO2003080660A2 (fr) 2003-10-02
WO2003080660A3 true WO2003080660A3 (fr) 2003-12-18

Family

ID=28051662

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2003/000207 Ceased WO2003080660A2 (fr) 2002-03-27 2003-03-26 Procede de preparation d'une proteine de liaison a l'heparine (hbp) mammifere recombinee

Country Status (2)

Country Link
AU (1) AU2003226907A1 (fr)
WO (1) WO2003080660A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150056653A1 (en) * 2011-07-29 2015-02-26 Georgia State University Research Foundation, Inc. Protein production method
CN108018294B (zh) * 2018-01-02 2020-11-17 昆明理工大学 一种三七植物激素结合蛋白基因PnPhBP1及应用
CN117343200B (zh) * 2023-12-04 2024-01-30 北京质肽生物医药科技有限公司 一种包含淀粉样核心多肽的融合蛋白、制备方法及其应用
CN117965667B (zh) * 2024-01-23 2024-08-16 陕西普罗安蒂生物科技发展有限公司 一种通过切割融合蛋白制备多肽的方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989008666A1 (fr) * 1988-03-17 1989-09-21 Novo-Nordisk A/S Proteines liant l'heparine, adn codant pour ces proteines, leurs procedes de production ainsi que des preparations therapeutiques les contenant
US5484885A (en) * 1989-07-05 1996-01-16 Emory University Chemotactic, antibiotic and lipopolysaccharide-binding peptide fragments of CAP37
WO1999000417A1 (fr) * 1997-06-25 1999-01-07 Novo Nordisk A/S Procede d'obtention de proteines fixant l'heparine dans des cellules de mammiferes
WO1999026647A1 (fr) * 1997-11-20 1999-06-03 Novo Nordisk A/S Utilisation de proteine de liaison a l'heparine pour la modulation ou la prophylaxie de l'apoptose des cellules de mammiferes
WO2000066627A1 (fr) * 1999-04-29 2000-11-09 Leukotech A/S Expression de la proteine de fixation d'heparine dans des cellules mammiferes de recombinaison

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989008666A1 (fr) * 1988-03-17 1989-09-21 Novo-Nordisk A/S Proteines liant l'heparine, adn codant pour ces proteines, leurs procedes de production ainsi que des preparations therapeutiques les contenant
US5484885A (en) * 1989-07-05 1996-01-16 Emory University Chemotactic, antibiotic and lipopolysaccharide-binding peptide fragments of CAP37
WO1999000417A1 (fr) * 1997-06-25 1999-01-07 Novo Nordisk A/S Procede d'obtention de proteines fixant l'heparine dans des cellules de mammiferes
WO1999026647A1 (fr) * 1997-11-20 1999-06-03 Novo Nordisk A/S Utilisation de proteine de liaison a l'heparine pour la modulation ou la prophylaxie de l'apoptose des cellules de mammiferes
WO2000066627A1 (fr) * 1999-04-29 2000-11-09 Leukotech A/S Expression de la proteine de fixation d'heparine dans des cellules mammiferes de recombinaison

Also Published As

Publication number Publication date
WO2003080660A2 (fr) 2003-10-02
AU2003226907A1 (en) 2003-10-08
AU2003226907A8 (en) 2003-10-08

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