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WO2003079793A1 - Composition a base d'extrait de l'espece zoanthus possedant une action anti-salissures et utilisation de cette composition - Google Patents

Composition a base d'extrait de l'espece zoanthus possedant une action anti-salissures et utilisation de cette composition Download PDF

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Publication number
WO2003079793A1
WO2003079793A1 PCT/IB2002/001153 IB0201153W WO03079793A1 WO 2003079793 A1 WO2003079793 A1 WO 2003079793A1 IB 0201153 W IB0201153 W IB 0201153W WO 03079793 A1 WO03079793 A1 WO 03079793A1
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WIPO (PCT)
Prior art keywords
extract
fractions
diatoms
composition
present
Prior art date
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Ceased
Application number
PCT/IB2002/001153
Other languages
English (en)
Inventor
Cynthia Olimpia Lydia Gonsalves
Chittur Thelakkat Achuthankutty
Perunninakulath Parameswaran Subrayan
Chandrakant Govind Naik
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Council of Scientific and Industrial Research CSIR
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Council of Scientific and Industrial Research CSIR
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Publication date
Application filed by Council of Scientific and Industrial Research CSIR filed Critical Council of Scientific and Industrial Research CSIR
Priority to KR10-2004-7015392A priority Critical patent/KR20040094886A/ko
Priority to CA002480866A priority patent/CA2480866A1/fr
Priority to JP2003577636A priority patent/JP2005520840A/ja
Priority to EP02717001A priority patent/EP1492407A1/fr
Priority to AU2002247922A priority patent/AU2002247922A1/en
Priority to PCT/IB2002/001153 priority patent/WO2003079793A1/fr
Publication of WO2003079793A1 publication Critical patent/WO2003079793A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10—Animals; Substances produced thereby or obtained therefrom

Definitions

  • the present invention relates to a composition useful as an anti-fouling agent by inhibiting microbial growth, said composition comprising extract and/or its fractions from Zoanthus sp. and optionally de-odorizing agents and a use of producing said extract and its fractions having about 100% anti-bacterial activity fouling microbes.
  • Zoanthids are benthic, sessile, colony forming organisms, comprising of thousands of individual polyps.
  • Several chemical compounds have been isolated from zoanthids, possessing various pharmacological properties (Moore and Scheuer, 1971, Science, 172, 495-498; Cimino et al., 1973, ; Sturaro et al., 1982; Rao et al., 1985, J.Org.Chem, 50, 3757-3760; Bakus et al., 1986, J.Chem.Ecol, 12, 951-987; Quinn, 1988, in Bioorganic Marine Chemistry, P.j. Scheuer ed. Vol. 2, 25-27; Shigemori et al., 1999, J Nat.
  • the main object of the present invention is to develop a composition comprising an extract from Zoanthus species.
  • Another main object of the present invention is to develop a composition having antifouling property. Yet another object of the present invention is to develop fractions from extract of animal Zoanthus species.
  • Still another object of the present invention is to develop a use of preparing composition comprising an extract from Zoanthus species. Still another object of the present invention is to develop a use of preparing said composition having 100% anti-bacterial activity.
  • Still another object of the present invention is to develop a use of preparing said composition with anti-microbial activity wherein, said activity is particularly effective against bacteria and diatoms.
  • the present invention relates to a composition useful as an anti-fouling agent by inhibiting microbial growth, said composition comprising extract and/or its fractions from Zoanthus sp. and optionally de-odorizing agents and a use of producing said extract and its fractions having about 100% anti-bacterial activity fouling microbes.
  • a composition useful as an anti-fouling agent by inhibiting microbial growth said composition comprising extract and/or its fractions from Zoanthus sp. and optionally de-odorizing agents and a use of producing said extract and its fractions having about 100% anti-bacterial activity fouling microbes.
  • the present invention relates to a composition useful as an anti-fouling agent by inhibiting microbial growth, said composition comprising extract and/or its fractions from Zoanthus sp. and optionally de-odorizing agents and a use of producing said extract and its fractions having about 100% anti-bacterial activity fouling microbes.
  • a composition comprising extract and/ or its fractions from Zoanthus sp, and optionally de-odorizing agents wherein said composition is useful as an anti-fouling agent against microbes.
  • extract and its fractions are prepared by using either polar or non-polar solvent.
  • animal species preferably used for said activity is Coelenterata Zoanthidea.
  • composition is prepared by using whole body tissue of said species.
  • de-odorizing agent shows no affect on the activity of said extract and fractions.
  • ratio of said extract and/or fractions and de-odorizing agent is ranging between 1: 1 to 1: 10.
  • said composition is particularly effective against bacteria and diatoms.
  • a use of producing an extract and its fractions from Zoanthus sp, useful as an anti-fouling agent by inhibiting microbial growth is provided.
  • determining anti-diatomic activity of crude extract and said fractions separately by placing diatoms with initial cell suspension of concentration ranging between 100 to 5000 cells/ml in flasks.
  • concentration of said extract against bacteria is preferably about 0.5 mg/mm of diameter disc.
  • said extract is incubated at preferably 28°C.
  • fractionating solvents are selected from a group comprising petroleum ether, chloroform, n-butanol, and aqueous.
  • dissolving non-polar fraction petroleum ether in acetone 0.005-. 01 ml.
  • bacteria are selected from a group comprising Bacillus cereus, B. circulans, pseudomonas vesicularis, and p. putida.
  • antibacterial activity of extract is 100%.
  • diatoms are selected from a group comprising Navicula subinflata and Nitzschia closterium.
  • n-butanol fraction shows about 100%
  • aqueous fraction shows inhibitory activity ranging between 70-85 % against diatoms at concentration ranging between 140-25 Oppm.
  • the present invention provides a process for the preparation of antifouling crude extract from Zoanthus sp and the said process comprising the steps of: i) Collection of live/fresh specimens of Zoanthus sp from intertidal rocks. ii) Washing the specimens clean with fresh sea water and soaking in methanol for about 10 days iii) Filtering the extract through Whatman filter paper I and vacuum evaporating at 40 U C to concentrate to crude residue.
  • the crude extract of Zoanthus sp is exhibiting antibacterial property.
  • the crude extract of Zoanthus sp is inhibiting growth of fouling bacteria such as Bacillus cereus, Bacillus circulans, Bacillus pumilus, Pseudomonas vesiciulans and Pseudomonas putida.
  • fouling bacteria such as Bacillus cereus, Bacillus circulans, Bacillus pumilus, Pseudomonas vesiciulans and Pseudomonas putida.
  • the crude extract of Zoanthus sp is inhibiting growth of fouling diatoms at all concentrations.
  • the crude extract of Zoanthus sp is completely inhibiting growth of diatoms, Navicula subinflata at and above 600 ppm concentration and Nitzschia closterium at and above 400 ppm concentration.
  • a process for fractionating the crude antifouling extract from Zoanthus sp based on increasing polarity of the solvents, viz. petroleum ether, chloroform, n-butanol and aqueous fractions and testing the antifouling property of all these fractions against the fouling diatoms Navicula subinflata and Nitzschia closterium comprising, obtaining crude extract from live/freshly collected
  • the crude extract of Zoanthus sp is fractionated based on increasing polarity of solvents into petroleum ether, chloroform, n-butanol and aqueous fractions.
  • all four fractions of the crude extract of Zoanthus sp are inhibiting the growth of fouling diatoms, Navicula subinflata and Nitzschia closterium.
  • the percentage inhibition in all the fraction is varying with concentration.
  • the petroleum ether fraction of the crude extract of Zoanthus sp is more effective in inhibiting growth of the fouling diatoms Navicula subinflata and Nitzschia closterium. In one more embodiment, the petroleum ether fraction of the crude extract of Zoanthus sp completely inhibits growth of Navicula subinflata at and above 160 ppm and Nitzschia closterium at and above 80 ppm concentrations.
  • a process for the preparation of an antifouling crude extract from Zoanthus sp and testing the said crude extract on fouling bacteria and diatoms also relates to a process for fractionation of the crude extract and testing the antifouling property of the fractions on fouling bacteria.
  • a process for the preparation of antifouling crude extract from Zoanthus sp and the said process comprising, Collection of live/fresh specimens of Zoanthus sp from intertidal rocks.
  • a process for fractionating the crude antifouling extract from Zoanthus sp based on increasing polarity of the solvents, viz. petroleum ether, chloroform, n-butanol and aqueous fractions and testing the antifouling property of all these fractions against fouling diatoms comprising, obtaining crude extract from live/freshly collected Zoanthus sp, washing them clean with sea water, soaking in methanol for about 10 days, filtering the extract through Whatman filter paper I and concentrating to crude residue under vacuum at 40°C.
  • the crude extract of Zoanthus sp is fractionated based on increasing polarity of solvents into petroleum ether, chloroform, n- butanol and aqueous fractions.
  • the petroleum ether fraction of the crude extract of Zoanthus sp completely inhibits growth of Navicula subinflata at and above 160 ppm and Nitzschia closterium at and above 80 ppm concentrations.
  • all fractions of the crude extract of Zoanthus Sp more particularly the petroleum ether fraction may have potential use as an antifouling agent in the aquatic environment
  • Zoanthus sp collected from Anjuna beach (Goa) was used for the invention.
  • the animals were carefully scrapped from the intertidal rocks during the low tide period using a metal spatula. About 2 kg wet weight of the specimens were used for the study.
  • the specimens were thoroughly washed with fresh seawater and soaked in methanol for 6-10 days for crude extract preparation. Extracts were obtained from the whole body tissue.
  • the solvent extract was decanted, filtered through Whatman filter paper I and vacuum evaporated at 40°C to concentrate to crude extract. About 30 gm of crude extract was thus obtained.
  • This example illustrates the useology used for preparation of the crude methanol extract from Zoanthus sp Example 2
  • bacterial isolates Five bacterial isolates, all belonging to the fouling community were used for the assay.
  • the bacterial assays were carried out by agar plate diffusion use (Amade et al., 1987, Mar. Biol, 94, 271-275) using Zobell marine agar (Himedia, Mumbai).
  • Bacterial strains used for the assay were Bacillus cereus B. circulans. B. pumilus, Pseudomonas vesicularis and P. putida. Concentrations of 0.5-mg/6mm-diameter disc of the extract prepared in methanol was introduced on sterilized filter paper discs.
  • Control flasks contained only the algae cells in medium. Flasks were incubated at room temperature (approx. 28°C) with a 12 hr light and 12 hours dark exposure conditions. Growth inhibition was determined after 6 days i.e., during the exponential growth phase in terms of the number of attached algal cells using a Sedgwick rafter and is expressed as % growth inhibition in Table 2. Extracts that were able to produce 50% growth inhibition were considered as active ones. This example illustrates methodology used for carrying out the/antidiatom assay/utilizing the crude Zoanthus sp.
  • the crude extract was then fractionated into four fractions using solvents of increasing polarity viz. petroleum ether, chloroform, n-butanol and aqueous. All fractions were tested for the activity as shown in Table 3 using the use mentioned above.
  • Control flasks for these sets contained cells plus acetone (equal volume added to make the stock solution) in the medium.
  • This example illustrates the methodology used for fractionation of the crude extract and their testing for the anti-diatom assay. TABLE 3 Percentage growth inhibition of diatoms at different concentrations of the four fractions of the crude extract of Zoanthus sp.
  • This example illustrates the methodology used for the treatment of results in the antidiatom assay using crude extract and fractions of Zoanthus sp.
  • the petroleum ether fraction was found to be more effective in inhibiting the growth of fouling diatoms.
  • This being a natural product may not have any toxic/adverse effects on non-target Organisms.

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Extraction Or Liquid Replacement (AREA)

Abstract

La présente invention concerne une composition utile en tant qu'agent anti-salissures agissant par blocage de la croissance microbienne, ladite composition comprenant de l'extrait et/ou des fractions d'extrait de l'espèce Zoanthus et éventuellement des agents désodorisants. L'invention concerne également l'utilisation de cet extrait et/ou de fractions de cet extrait pour produire un agent possédant une activité antibactérienne totale contre les salissures.
PCT/IB2002/001153 2002-03-26 2002-03-26 Composition a base d'extrait de l'espece zoanthus possedant une action anti-salissures et utilisation de cette composition Ceased WO2003079793A1 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
KR10-2004-7015392A KR20040094886A (ko) 2002-03-26 2002-03-26 항-오염 활성을 갖는 조안투스 종 추출물을 포함하는조성물 및 그의 용도
CA002480866A CA2480866A1 (fr) 2002-03-26 2002-03-26 Composition a base d'extrait de l'espece zoanthus possedant une action anti-salissures et utilisation de cette composition
JP2003577636A JP2005520840A (ja) 2002-03-26 2002-03-26 抗汚染活性をするスナギンチャク種を抽出物含む組成物およびこれらの使用
EP02717001A EP1492407A1 (fr) 2002-03-26 2002-03-26 Composition a base d'extrait de l'espece zoanthus possedant une action anti-salissures et utilisation de cette composition
AU2002247922A AU2002247922A1 (en) 2002-03-26 2002-03-26 Composition comprising zoanthus sp. extract with anti-fouling acrtivity and a use thereof
PCT/IB2002/001153 WO2003079793A1 (fr) 2002-03-26 2002-03-26 Composition a base d'extrait de l'espece zoanthus possedant une action anti-salissures et utilisation de cette composition

Applications Claiming Priority (1)

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PCT/IB2002/001153 WO2003079793A1 (fr) 2002-03-26 2002-03-26 Composition a base d'extrait de l'espece zoanthus possedant une action anti-salissures et utilisation de cette composition

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EP (1) EP1492407A1 (fr)
JP (1) JP2005520840A (fr)
KR (1) KR20040094886A (fr)
AU (1) AU2002247922A1 (fr)
CA (1) CA2480866A1 (fr)
WO (1) WO2003079793A1 (fr)

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AR124979A1 (es) * 2021-03-01 2023-05-24 Sumitomo Chemical Co Método para el control de artrópodos dañinos o nematodos dañinos usando zoantaminas

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04295401A (ja) * 1991-03-22 1992-10-20 Kaiyo Bio Technol Kenkyusho:Kk 水中付着生物忌避剤
WO1995016000A1 (fr) * 1993-12-09 1995-06-15 The Research Foundation Of State University Of New York Procedes et peintures anti fouling marines
EP1138690A1 (fr) * 2000-03-31 2001-10-04 Council Of Scientific And Industrial Research Un procédé de purification de 2-déoxyecdysterone de Zoanthus sp, et son emploi pour la production d'un medicament pour le déclenchement de l'accouchement ou pour l'interruption de la grossesse

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04295401A (ja) * 1991-03-22 1992-10-20 Kaiyo Bio Technol Kenkyusho:Kk 水中付着生物忌避剤
WO1995016000A1 (fr) * 1993-12-09 1995-06-15 The Research Foundation Of State University Of New York Procedes et peintures anti fouling marines
EP1138690A1 (fr) * 2000-03-31 2001-10-04 Council Of Scientific And Industrial Research Un procédé de purification de 2-déoxyecdysterone de Zoanthus sp, et son emploi pour la production d'un medicament pour le déclenchement de l'accouchement ou pour l'interruption de la grossesse

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
ATTA-UR-RAHMAN: "Zoanthaminone, a New Alakloid from a Marine Zoanthid", TETRAHEDRON LETTERS, vol. 30, no. 49, 1989, pages 6825 - 6828, XP001105364 *
C.BHEEMASANKARA RAO ET.AL.: "Alkaloids from a Marine Zoanthid", JOURNAL OF ORGANIC CHEMISTRY, vol. 50, no. 20, 1985, pages 3757 - 3760, XP001105045 *
C.BHEEMASANKARA RAO ET.AL.: "Zoanthamine: A novel Alkaloid from a Marine Zoanthid", JOUNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 106, no. 25, 1984, pages 7983 - 7984, XP001105048 *
C.BHEEMASANKARA RAO: "Two new Alkaloids from an Indian species of Zoanthus", HETEROCYCLES, vol. 28, no. 1, 1989, pages 103 - 106, XP001105368 *
C.O.L.GONSALVES: "Effect of holothurian and zoanthid extracts on growth of some bacterial and diatom species", INDIAN JOURNAL OF MARINE SCIENCE, vol. 26, 1997, pages 377 - 379, XP001105036 *
DATABASE WPI Section Ch Week 199248, Derwent World Patents Index; Class C03, AN 1992-395333, XP002213670 *
H.SHIGEMORI ET.AL.: "Palythoalones A and B, New Ecdysteroids from the Marine Zoanthid Palythoa australiae", JOURNAL OF NATURAL PRODUCTS, vol. 62, no. 2, 1999, pages 372 - 374, XP001105046 *
MOORE & SCHEUER: "Palytoxin: A New Marine Toxin from a Coelenterate", SCIENCE, vol. 172, 1971, pages 495 - 498, XP001105042 *
S.FUKUZAWA ET.AL.: "The Isolation and Structures of Five New Alkaloids, Norzoanthamine, Oxyzoanthamine, Norzoanthaminone, Cyclozoanthamine and Epinorzoanthamine", HETEROCYCLIC COMMUNICATIONS, vol. 1, no. 2-3, 1995, pages 207 - 214, XP001105369 *
U.BABU ET.AL.: "Hariamide, a novel sulphated sphingolipid from a Zoanthus sp. of the Indian coast", JOURNAL OF NATURAL PRODUCTS, vol. 60, no. 12, 1997, pages 1307 - 1309, XP001098861 *

Also Published As

Publication number Publication date
KR20040094886A (ko) 2004-11-10
JP2005520840A (ja) 2005-07-14
CA2480866A1 (fr) 2003-10-02
EP1492407A1 (fr) 2005-01-05
AU2002247922A1 (en) 2003-10-08

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