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WO2003078585A3 - Compositions and methods for primate neural cell production - Google Patents

Compositions and methods for primate neural cell production Download PDF

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Publication number
WO2003078585A3
WO2003078585A3 PCT/US2003/007665 US0307665W WO03078585A3 WO 2003078585 A3 WO2003078585 A3 WO 2003078585A3 US 0307665 W US0307665 W US 0307665W WO 03078585 A3 WO03078585 A3 WO 03078585A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
primate
cell production
compositions
neural cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/007665
Other languages
French (fr)
Other versions
WO2003078585A2 (en
Inventor
Steve Stice
John Calhoun
Ian Lyons
Maisam Mitalipova
Allan Robins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hospira Adelaide Pty Ltd
University of Georgia Research Foundation Inc UGARF
Original Assignee
Bresagen Ltd
University of Georgia Research Foundation Inc UGARF
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bresagen Ltd, University of Georgia Research Foundation Inc UGARF filed Critical Bresagen Ltd
Priority to AU2003224681A priority Critical patent/AU2003224681A1/en
Publication of WO2003078585A2 publication Critical patent/WO2003078585A2/en
Publication of WO2003078585A3 publication Critical patent/WO2003078585A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/04—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from germ cells

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides compositions and methods for primate neural cell production. More particularly, the present invention provides cellular differentiation methods employing a MEDII conditioned medium or an essentially serum free differentiation medium for the generation of primate neural cells from pluripotent primate stem cells.
PCT/US2003/007665 2002-03-13 2003-03-13 Compositions and methods for primate neural cell production Ceased WO2003078585A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003224681A AU2003224681A1 (en) 2002-03-13 2003-03-13 Compositions and methods for primate neural cell production

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36438102P 2002-03-13 2002-03-13
US60/364,381 2002-03-13

Publications (2)

Publication Number Publication Date
WO2003078585A2 WO2003078585A2 (en) 2003-09-25
WO2003078585A3 true WO2003078585A3 (en) 2003-12-24

Family

ID=28041906

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/007665 Ceased WO2003078585A2 (en) 2002-03-13 2003-03-13 Compositions and methods for primate neural cell production

Country Status (2)

Country Link
AU (1) AU2003224681A1 (en)
WO (1) WO2003078585A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004015077A2 (en) * 2002-08-08 2004-02-19 University Of Georgia Research Foundation, Inc. Compositions and methods for neural differentiation of embryonic stem cells
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
AU2015200780B2 (en) * 2003-12-23 2017-03-02 Viacyte, Inc. Definitive endoderm

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6458589B1 (en) * 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
US20020151054A1 (en) * 2001-03-02 2002-10-17 Rathjen Peter David Cellular production control
US20030134413A1 (en) * 2000-01-14 2003-07-17 Rathjen Peter David Cell production
US20030162290A1 (en) * 2002-01-25 2003-08-28 Kazutomo Inoue Method for inducing differentiation of embryonic stem cells into functioning cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030134413A1 (en) * 2000-01-14 2003-07-17 Rathjen Peter David Cell production
US6458589B1 (en) * 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
US6506574B1 (en) * 2000-04-27 2003-01-14 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
US20020151054A1 (en) * 2001-03-02 2002-10-17 Rathjen Peter David Cellular production control
US20030162290A1 (en) * 2002-01-25 2003-08-28 Kazutomo Inoue Method for inducing differentiation of embryonic stem cells into functioning cells

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
CALHOUN ET AL.: "Differentiation of rhesus embryonic stem cells to neural progenitors and neurons", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 306, no. 1, 20 June 2003 (2003-06-20), pages 191 - 197, XP002972823 *
ITSKOVITZ-ELDOR ET AL.: "Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germ layers", MOLECULAR MEDICINE, vol. 6, no. 2, February 2000 (2000-02-01), pages 88 - 95, XP000942869 *
KUO ET AL.: "Differentiation of Monkey embryonic stem cells into neural lineages", BIOLOGY OF REPRODUCTION, vol. 68, 11 December 2002 (2002-12-11), pages 1727 - 1735, XP002972824 *
MARTIN ET AL.: "Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro", PROC. NATL. ACAD. SCI. USA, vol. 72, no. 4, April 1975 (1975-04-01), pages 1441 - 1445, XP000985468 *
PERLINGEIRO ET AL.: "Clonal analysis of differentiating embryonic stem cells reveals a hematopoietic progenitor with primitive erythroid and adult lymphoid-myeloid potential", DEVELOPMENT, vol. 128, no. 22, November 2001 (2001-11-01), pages 4597 - 4604, XP002972825 *
RATHJEN ET AL., 6 August 2003, BOR PAPERS IN PRESS, article "Identification of a biological activity that supports maintenance and proliferation of pluripotent cells from the primitive ectoderm of the mouse", pages: 1 - 38, XP002972822 *
RATHJEN ET AL.: "Formation of a primitive ectoderm like cell population, EPL cells, from ES cells in response to biologically derived factors", JOURNAL OF CELL SCIENCE, vol. 112, March 1999 (1999-03-01), pages 601 - 612, XP000986695 *
YUEN ET AL.: "Generation of a primitive erythroid cell line and promotion of its growth by basic fibroblast growth factor", BLOOD, vol. 91, no. 9, 1 May 1998 (1998-05-01), pages 3202 - 3209, XP002939897 *

Also Published As

Publication number Publication date
AU2003224681A1 (en) 2003-09-29
AU2003224681A8 (en) 2003-09-29
WO2003078585A2 (en) 2003-09-25

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